CN112553095A - Compound microbial inoculum for treating high-concentration kitchen wastewater and preparation method thereof - Google Patents
Compound microbial inoculum for treating high-concentration kitchen wastewater and preparation method thereof Download PDFInfo
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- CN112553095A CN112553095A CN202110026830.1A CN202110026830A CN112553095A CN 112553095 A CN112553095 A CN 112553095A CN 202110026830 A CN202110026830 A CN 202110026830A CN 112553095 A CN112553095 A CN 112553095A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
Abstract
A preparation method of a compound microbial inoculum for treating high-concentration kitchen wastewater is characterized in that the strain composition of the compound microbial inoculum is as follows: cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae; the preparation method comprises the following steps: s1: respectively activating cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae; s2: respectively preparing corresponding bacterial suspensions from the activated strains; s3: the prepared cryptococcus suspension, lipolytica candida suspension, tropical candida suspension and saccharomyces cerevisiae suspension are mixed according to the volume ratio of 1-5: 1-3: 1-2: 1, preparing a compound microbial inoculum; the compound microbial inoculum can efficiently treat the kitchen wastewater, effectively reduce COD, ammonia nitrogen and total phosphorus in the wastewater, absorb and digest various organic matters and inorganic matters, effectively purify the kitchen wastewater, and has good environmental benefit.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and particularly relates to a preparation method of a compound microbial inoculum for treating high-concentration kitchen wastewater.
Background
With the continuous improvement of the living quality of residents in China, the diversification of food types in daily life and the increasing of waste phenomena, the yield of kitchen waste is increased, and according to statistics, the kitchen waste accounts for 30% -50% of the total amount of municipal solid waste in the world. The water content of the kitchen waste is generally 80% -90%, so that a large amount of kitchen waste water can be generated in the pretreatment processes of draining, sorting, crushing, pulping, three-phase separation and the like of the kitchen waste, and theoretically, 800-900 kg of kitchen waste can be generated by 1 ton of the kitchen waste. The kitchen waste water has the characteristics of high suspended matter, high chemical oxygen demand, high biochemical oxygen demand, high salinity, easy deterioration and the like; the currently commonly used methods for treating kitchen wastewater include physical and chemical methods such as air floatation method and electrochemical method, chemical methods such as chemical oxidation method and neutralization method, and biological treatment methods such as activated sludge method and biological filter method, but both physical and chemical methods have the disadvantages of high cost and easy secondary pollution, while the biological method has the characteristics of large treatment capacity, economic feasibility, no secondary pollution and the like, and is favored by researchers.
However, when the microbial inoculum used in the existing biological treatment method is used for treating high-concentration kitchen wastewater, although the microbial inoculum has a certain degradation effect, the microbial inoculum is inhibited by high salt and high grease, and the degradation capability of the microbial inoculum on various organic matters and inorganic matters is weak.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a compound microbial inoculum for treating high-concentration kitchen wastewater and a preparation method thereof, which can effectively solve the problems in the background technology.
In order to solve the problems, the technical scheme adopted by the invention is as follows: a complex microbial inoculum for treating high-concentration kitchen wastewater and a preparation method thereof are characterized in that the strain composition of the complex microbial inoculum is as follows: cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae; the preparation method comprises the following steps:
s1: respectively activating cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae;
s2: respectively preparing corresponding bacterial suspensions from the activated cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae strains;
s3: the prepared cryptococcus suspension, lipolytica candida suspension, tropical candida suspension and saccharomyces cerevisiae suspension are mixed according to the volume ratio of 1-5: 1-3: 1-2: 1 preparing a compound microbial inoculum.
As a further preferable embodiment of the present invention, the step S1 in the preparation method comprises:
s11: inoculating the cryptococcus rhodochrous freeze-dried powder into 50-2000 mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 20-38 ℃ for 12-36 h at the rotating speed of 100-200 r/min to complete the activation of the cryptococcus rhodochrous;
s12: inoculating the candida lipolytica freeze-dried powder into 50-2000 mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 20-38 ℃ for 12-36 h at the rotating speed of 100-200 r/min to complete activation of the candida lipolytica;
s13: inoculating the candida tropicalis freeze-dried powder into 50-2000 mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 20-38 ℃ for 12-36 h at the rotating speed of 100-200 r/min to complete the activation of the candida tropicalis;
s14: inoculating the saccharomyces cerevisiae freeze-dried powder into 50-2000 mL of a yeast leaching peptone glucose culture medium, and culturing at a constant temperature of 20-38 ℃ for 12-36 h at a rotating speed of 100-200 r/min to complete activation of the saccharomyces cerevisiae.
As a further preferable embodiment of the present invention, the step S2 in the preparation method comprises:
s21: 0.2-20 mL of activated cryptococcus is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the cryptococcus suspension is obtained after the thermostatic culture for 24h at the rotation speed of 150r/min and the temperature of 30 ℃;
s22: 0.2-20 mL of activated lipolytic Candida is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out at the constant temperature of 30 ℃ for 24h at the rotating speed of 150r/min, so as to obtain lipolytic Candida yeast suspension;
s23: 0.2-20 mL of activated candida tropicalis is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out at a constant temperature of 30 ℃ for 24h at a rotating speed of 150r/min, so as to obtain candida tropicalis suspension;
s24: and (3) leaching 0.2-20 mL of activated saccharomyces cerevisiae in 200mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 30 ℃ for 24h at the rotating speed of 150r/min to obtain saccharomyces cerevisiae suspension.
As a further preferable embodiment of the present invention, in step S3 of the preparation method, the prepared cryptococcus suspension, candida lipolytica suspension, candida tropicalis suspension and saccharomyces cerevisiae suspension are mixed according to a volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
As a further preferable embodiment of the present invention, in step S3 of the preparation method, the prepared cryptococcus suspension, candida lipolytica suspension, candida tropicalis suspension and saccharomyces cerevisiae suspension are mixed according to a volume ratio of 5: 3: 2: 1, mixing to obtain the compound microbial inoculum.
In a further preferred embodiment of the present invention, the yeast extract peptone glucose medium comprises the following components: glucose, yeast extract powder, peptone and deionized water; and each 1L of yeast extract peptone glucose culture medium comprises the following components in proportion: glucose 0.1-10%, yeast extract powder 0.1-1%, peptone 0.1-1%; the pH value of the yeast extract peptone glucose culture medium is 4.0-9.0.
As a further preferred embodiment of the invention, the freeze-dried powders of Cryptococcus, Candida lipolytica, Candida tropicalis and Saccharomyces cerevisiae strains are purchased from Guangdong province center for culture Collection of microorganisms and are numbered GIM2.205, GIM2.187, GIM2.147 and GIM2.207 respectively.
Compared with the prior art, the invention provides a compound microbial inoculum for treating high-concentration kitchen wastewater and a preparation method thereof, and the compound microbial inoculum has the following beneficial effects:
according to the invention, cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae are prepared into the compound microbial inoculum, and the compound microbial inoculum can be used for efficiently treating kitchen wastewater, effectively reducing COD, ammonia nitrogen and total phosphorus in the wastewater, absorbing and digesting various organic matters and inorganic matters, effectively purifying the kitchen wastewater, and has good environmental benefits.
Detailed Description
The present invention will be described in further detail with reference to the following detailed description of exemplary embodiments, which is to be construed as merely illustrative, and not restrictive, of the principles of the invention.
The kitchen waste water used in the embodiment is taken from a certain kitchen waste treatment station in Guangzhou Caryun district, and the water quality condition is TDS =8550mg/L, COD =87700mg/L, ammonia nitrogen =480mg/L, total phosphorus =200mg/L, animal and vegetable oil =3900mg/L, pH = 3.3.
Example 1
1. Preparation of compound microbial inoculum
Lyophilized powders of Cryptococcus, Candida lipolytica, Candida tropicalis and Saccharomyces cerevisiae strains were purchased from Guangdong province culture Collection and numbered GIM2.205, GIM2.187, GIM2.147 and GIM 2.207.
A. Activation of Cryptococcus rhodochrous: inoculating the cryptococcus rhodochrous freeze-dried powder into 50mL yeast extract peptone glucose culture medium, and culturing at the constant temperature of 30 ℃ for 24h at the rotating speed of 150 r/min.
B. Activation of lipolytic candida: inoculating the Candida lipolytica freeze-dried powder into 50mL yeast extract peptone glucose culture medium, and culturing at the constant temperature of 30 ℃ for 24h at the rotating speed of 150 r/min.
C. Activation of candida tropicalis: inoculating the Candida tropicalis freeze-dried powder into 50mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 30 ℃ for 24h at the rotating speed of 150 r/min.
D. Activation of saccharomyces cerevisiae: inoculating the saccharomyces cerevisiae freeze-dried powder into a 50mL yeast extract peptone glucose culture medium, and culturing at the constant temperature of 30 ℃ for 24h at the rotating speed of 150 r/min.
E. Preparing the cryptococcus suspension: 1mL of activated cryptococcus was soaked in 200mL of yeast extract peptone glucose medium and incubated at 30 ℃ for 24h at a rotation speed of 150 r/min.
F. Preparing a candida lipolytica suspension: 1mL of activated lipolytic Candida is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out for 24h at the constant temperature of 30 ℃ at the rotating speed of 150 r/min.
G. Preparation of candida tropicalis suspension: 1mL of activated candida tropicalis is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out for 24h at the constant temperature of 30 ℃ at the rotating speed of 150 r/min.
H. Preparing a saccharomyces cerevisiae suspension: 1mL of activated saccharomyces cerevisiae is taken out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out for 24h at the constant temperature of 30 ℃ at the rotating speed of 150 r/min.
I. E, mixing the cryptococcus suspension, the lipolysis candida suspension, the tropical candida suspension and the saccharomyces cerevisiae suspension obtained in the step E to step H according to the volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
J. The yeast extract peptone glucose culture medium comprises the following components: glucose, yeast extract powder, peptone and deionized water; and each 1L of yeast extract peptone glucose culture medium comprises the following components in proportion: glucose 0.1-10%, yeast extract powder 0.1-1%, peptone 0.1-1%; the pH value of the yeast extract peptone glucose culture medium is 4.0-9.0.
2. Treatment of kitchen wastewater
A. Sampling kitchen wastewater: and (3) collecting kitchen waste water by adopting a sampler at a sewage discharge port of the kitchen waste treatment station, and continuously sampling for 0.5 h.
B. Diluting kitchen wastewater: 100mL, 40mL and 20mL of kitchen wastewater are respectively transferred into 3 conical flasks with 500mL, and the conical flasks are diluted to 200mL by deionized water, namely, each wastewater is diluted by 2 times, 5 times and 10 times.
C. Treating kitchen wastewater by using a compound microbial inoculum: and respectively adding 24ml of compound microbial inoculum into each conical flask, and then carrying out constant temperature treatment on the conical flasks at 28 ℃ and the rotating speed of 150r/min for 72 h. After the treatment was completed, the COD of the wastewater was measured.
D. The experimental results are as follows: the kitchen wastewater is treated by using a compound microbial inoculum prepared from cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae, so that the synergistic effect and population advantages of various strains can be exerted, and the high-salt and high-grease environment can be better tolerated. For kitchen wastewater diluted by 2 times, the removal rate of COD reaches 85%; for kitchen wastewater diluted by 5 times, the removal rate of COD can reach 95%; for kitchen wastewater diluted by 10 times, the removal rate of COD can reach 93%.
Example 2
1. Preparation of compound microbial inoculum
The same as in example 1.
2. Treatment of kitchen wastewater
A. Sampling kitchen wastewater: and (3) collecting kitchen waste water by adopting a sampler at a sewage discharge port of the kitchen waste treatment station, and continuously sampling for 0.5 h.
B. Physicochemical pretreatment of kitchen wastewater: adding 0.1% of polyaluminium chloride into the original kitchen wastewater, stirring for 3 minutes, adding lime to adjust the pH value to 9.0, stirring for 2 minutes, adding 1% of 5% polyacrylamide, stirring for 1 minute, standing for precipitation until layering, carrying out solid-liquid separation, and taking the supernatant for later use.
C. Diluting kitchen wastewater: 100mL of the supernatant was taken in a culture flask and diluted to 1000 mL.
D. Treating kitchen wastewater by using a compound microbial inoculum: adding 4mL of compound microbial inoculum into a culture bottle, placing the culture bottle in a constant-temperature water bath at 30 ℃, continuously aerating for 21d, collecting 10mL of water samples (stored at 4 ℃) when 1 st, 2 nd, 3 rd, 4 th, 5 th, 6 th, 7 th, 8 th, 9 th, 12 th, 13 th, 14 th, 17 th and 21 th days are 7 th in the morning, directly measuring and recording the pH value of the water sample by using a pH meter, and adding deionized water to 1000mL after sampling is finished every time so as to supplement naturally evaporated water. After the sampling of the sample was completed, the COD of the sample was measured over 24 h.
E. The experimental results are as follows: 3d before the experiment begins, the COD removal rate of the kitchen wastewater reaches 50 percent; when the experiment of 21d is finished, the COD concentration is reduced to 400mg/L, and the removal rate reaches 99 percent; in the whole experimental process, the ammonia nitrogen removal rate is up to 95%, and the total phosphorus removal rate is up to 96%.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (7)
1. A preparation method of a compound microbial inoculum for treating high-concentration kitchen wastewater is characterized in that the strain composition of the compound microbial inoculum is as follows: cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae; the preparation method comprises the following steps:
s1: respectively activating cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae;
s2: respectively preparing corresponding bacterial suspensions from the activated cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae strains;
s3: the prepared cryptococcus suspension, lipolytica candida suspension, tropical candida suspension and saccharomyces cerevisiae suspension are mixed according to the volume ratio of 1-5: 1-3: 1-2: 1 preparing a compound microbial inoculum.
2. The preparation method of the complex microbial inoculum for treating high-concentration kitchen wastewater according to claim 1, wherein the step S1 in the preparation method comprises the following steps:
s11: inoculating the cryptococcus rhodochrous freeze-dried powder into 50-2000 mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 20-38 ℃ for 12-36 h at the rotating speed of 100-200 r/min to complete the activation of the cryptococcus rhodochrous;
s12: inoculating the candida lipolytica freeze-dried powder into 50-2000 mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 20-38 ℃ for 12-36 h at the rotating speed of 100-200 r/min to complete activation of the candida lipolytica;
s13: inoculating the candida tropicalis freeze-dried powder into 50-2000 mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 20-38 ℃ for 12-36 h at the rotating speed of 100-200 r/min to complete the activation of the candida tropicalis;
s14: inoculating the saccharomyces cerevisiae freeze-dried powder into 50-2000 mL of a yeast leaching peptone glucose culture medium, and culturing at a constant temperature of 20-38 ℃ for 12-36 h at a rotating speed of 100-200 r/min to complete activation of the saccharomyces cerevisiae.
3. The preparation method of the complex microbial inoculum for treating high-concentration kitchen wastewater according to claim 1, wherein the step S2 in the preparation method comprises the following steps:
s21: 0.2-20 mL of activated cryptococcus is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the cryptococcus suspension is obtained after the thermostatic culture for 24h at the rotation speed of 150r/min and the temperature of 30 ℃;
s22: 0.2-20 mL of activated lipolytic Candida is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out at the constant temperature of 30 ℃ for 24h at the rotating speed of 150r/min, so as to obtain lipolytic Candida yeast suspension;
s23: 0.2-20 mL of activated candida tropicalis is taken to be soaked out of a peptone glucose culture medium in 200mL of yeast, and the culture is carried out at a constant temperature of 30 ℃ for 24h at a rotating speed of 150r/min, so as to obtain candida tropicalis suspension;
s24: and (3) leaching 0.2-20 mL of activated saccharomyces cerevisiae in 200mL of yeast extract peptone glucose culture medium, and culturing at the constant temperature of 30 ℃ for 24h at the rotating speed of 150r/min to obtain saccharomyces cerevisiae suspension.
4. The method for preparing a recompounded microbial inoculum for treating high-concentration kitchen wastewater as claimed in claim 1, wherein the preparation method step S3 is to mix the prepared cryptococcus suspension, candida lipolytica suspension, candida tropicalis suspension and saccharomyces cerevisiae suspension according to a volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
5. The method for preparing a recompounded microbial inoculum for treating high-concentration kitchen wastewater as claimed in claim 1, wherein the preparation method step S3 is to mix the prepared cryptococcus suspension, candida lipolytica suspension, candida tropicalis suspension and saccharomyces cerevisiae suspension according to a volume ratio of 5: 3: 2: 1, mixing to obtain the compound microbial inoculum.
6. The preparation method of the complex microbial inoculum for treating high-concentration kitchen wastewater according to claim 2 or 3, wherein a yeast extract peptone glucose medium comprises the following components: glucose, yeast extract powder, peptone and deionized water; and each 1L of yeast extract peptone glucose culture medium comprises the following components in proportion: glucose 0.1-10%, yeast extract powder 0.1-1%, peptone 0.1-1%; the pH value of the yeast extract peptone glucose culture medium is 4.0-9.0.
7. The preparation method of the complex microbial inoculum for treating high-concentration kitchen wastewater as claimed in claim 1, wherein the freeze-dried powder of cryptococcus, candida lipolytica, candida tropicalis and saccharomyces cerevisiae strains are purchased from Guangdong province microorganism culture collection and are correspondingly numbered as GIM2.205, GIM2.187, GIM2.147 and GIM 2.207.
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