CN114162978A - Application of cryptococcus oxytetracycline in wastewater treatment - Google Patents
Application of cryptococcus oxytetracycline in wastewater treatment Download PDFInfo
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- 241001337994 Cryptococcus <scale insect> Species 0.000 title claims abstract description 16
- 239000004100 Oxytetracycline Substances 0.000 title claims abstract description 9
- IWVCMVBTMGNXQD-PXOLEDIWSA-N oxytetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-PXOLEDIWSA-N 0.000 title claims abstract description 9
- 229960000625 oxytetracycline Drugs 0.000 title claims abstract description 9
- 235000019366 oxytetracycline Nutrition 0.000 title claims abstract description 9
- IWVCMVBTMGNXQD-UHFFFAOYSA-N terramycin dehydrate Natural products C1=CC=C2C(O)(C)C3C(O)C4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-UHFFFAOYSA-N 0.000 title claims abstract description 9
- 238000004065 wastewater treatment Methods 0.000 title abstract description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 26
- 239000011574 phosphorus Substances 0.000 claims abstract description 26
- 241000222050 Vanrija humicola Species 0.000 claims abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 2
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 239000002351 wastewater Substances 0.000 abstract description 55
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 25
- 239000000126 substance Substances 0.000 abstract description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 10
- 229910052760 oxygen Inorganic materials 0.000 abstract description 10
- 239000001301 oxygen Substances 0.000 abstract description 10
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 241000157049 Microtus richardsoni Species 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- 238000004519 manufacturing process Methods 0.000 description 21
- 238000000855 fermentation Methods 0.000 description 20
- 230000004151 fermentation Effects 0.000 description 20
- 239000006228 supernatant Substances 0.000 description 18
- 241001369618 Papiliotrema terrestris Species 0.000 description 13
- 238000000034 method Methods 0.000 description 12
- 239000002609 medium Substances 0.000 description 11
- 241000123447 Solicoccozyma terreus Species 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- 240000006394 Sorghum bicolor Species 0.000 description 9
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000000843 powder Substances 0.000 description 8
- 238000002835 absorbance Methods 0.000 description 7
- 238000006731 degradation reaction Methods 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241001052560 Thallis Species 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 description 3
- 239000012490 blank solution Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000013048 microbiological method Methods 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000013124 brewing process Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
- C02F2103/325—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters from processes relating to the production of wine products
Abstract
The invention discloses a cryptococcus oxytoca (A. terrestris)Cryptococcus humicolus) The new application of BSLL1-1 in wastewater treatment, namely the application of BSLL1-1 in reducing COD, ammonia nitrogen and total phosphorus content of brewing wastewater; experiments prove that the strain can effectively reduce COD (chemical oxygen demand), total phosphorus and ammonia nitrogen content in brewing wastewater, and can further improve the treatment effect of cryptococcus oxytetracycline on the wastewater by adjusting the carbon-nitrogen ratio of the wastewater, so that the cryptococcus oxytetracycline BSLL1-1 has better application prospect in the wastewater treatment, and the cryptococcus oxytetracycline BSLL1-1 is simple to prepare, easy to store, convenient to use and suitable for industrial productionAnd market popularization and application.
Description
Technical Field
The invention relates to the technical field of biological treatment of brewing wastewater, in particular to a method for reducing Chemical Oxygen Demand (Chemical Oxygen Demand COD), ammonia nitrogen and total phosphorus content in brewing wastewater by using cryptococcus terreus.
Background
Chemical Oxygen Demand (Chemical Oxygen Demand COD) refers to the amount of oxidant consumed by oxidized substances in a water body after oxidation, and can represent the content of reducing substances in a water sample. The reducing substances in the water body are various, including nitrite, organic matters and the like, wherein the organic matters account for the main body, so COD is also used as an important index for measuring the content of the organic matters in the water body. In the degradation process of organic matters, a large amount of dissolved oxygen is consumed to cause the shortage of oxygen content in a water body, so that not only can the ecological balance of the environment and a biological community be damaged, but also the water quality is deteriorated. If the dissolved oxygen in the water is consumed, some anaerobic bacteria will multiply in large quantities, eventually leading to the water becoming black and odorous. In addition, once water sample (industrial wastewater) with high COD content enters into natural water body, it may cause chronic poisoning due to the existence of volatile irritant substances (such as aromatic compounds). Meanwhile, if the total phosphorus and ammonia nitrogen content in the wastewater exceeds the standard, eutrophication of the water body can be caused, and the algae can be greatly propagated when discharged into the water body, so that the water quality is deteriorated.
The brewing raw materials mainly comprise crops, so the wastewater generated in the brewing process also mainly comprises organic wastewater. During the process, 14-16m can be produced for each 1 ton of wine3The content of organic matters in the distiller's grains liquid reaches about 33000-35000 mg/L, and if the organic matters are directly discharged, serious water pollution is caused, so that how to treat the brewing wastewater is very important. The prior brewing wastewater treatment technology comprises an electrolysis method, a flocculation method, a microbiological method and the like, the microbiological method for treating the brewing wastewater is relatively energy-saving and environment-friendly, and no related report that cryptococcus terrestris is utilized to treat the brewing fermentation wastewater so as to degrade the COD content in the water body exists at home and abroad.
Disclosure of Invention
The invention provides a method for preparing cryptococcus neoformansCryptococcus humicolus) BSLL1-1 is a method for reducing COD, ammonia nitrogen and total phosphorus content in the brewing wastewater;
cryptococcus terrestris (C.terrestris) referred to in the present applicationCryptococcus humicolus) The strain BSLL1-1 was disclosed in the non-patent literature prior to the filing date of the present application, namely in the "Isolation of Al-tolerance layers and identification of the same Al-tolerance chainteristics. Journal of Biological Research-Thessaloniki 18: 227-.
The brewing wastewater in the invention comprises sorghum liquor production wastewater and liquor production wastewater.
The purpose of the invention is realized by the following technical scheme:
(1) activating strains: carrying out YPD slant culture and YPD seed culture on Cryptococcus oxytoca BSLL1-1 to obtain an activated seed solution; the temperature of slant culture was 28 ℃, the culture time was 48h, YPD slant medium (g/L): yeast extract powder 10, peptone 20, glucose 20 and agar powder 20; seed culture temperature 28 deg.C, shaking culture at 180rpm for 24h, YPD seed culture medium (g/L): yeast extract powder 10, peptone 20, and glucose 20;
(2) preparation of a fermentation medium: precipitating the brewing wastewater for 24h, and taking the supernatant as a fermentation medium directly; simultaneously, adding pure water with the same volume as the brewing wastewater, and uniformly mixing the pure water and the brewing wastewater to serve as a fermentation culture medium;
(3) inoculating cryptococcus terrestris seed liquid into a sterilized wastewater fermentation culture medium according to the inoculation amount of 15%, and performing shake culture at 28 ℃ and 180rpm to obtain fermentation liquid;
(4) and measuring COD (chemical oxygen demand), total phosphorus and ammonia nitrogen contents in the brewing wastewater which is not treated by the cryptococcus terreus and fermented by the cryptococcus terreus, and calculating the removal rate.
The invention has the advantages and technical effects that:
the invention provides a new application approach for cryptococcus oxytoca BSLL1-1, and the invention applies cryptococcus oxytoca BSLL1-1 to the reduction of COD, total phosphorus and ammonia nitrogen content in brewing wastewater, and experiments show that the contents of COD, total phosphorus and ammonia nitrogen in the two types of wastewater can be reduced by fermenting and culturing wild cryptococcus oxytoca BSLL1-1 in sorghum liquor production wastewater and white spirit production wastewater. Taking a kaoliang spirit production wastewater stock solution as a fermentation medium, using glucose as a carbon source and yeast powder as a nitrogen source, adjusting the carbon-nitrogen ratio to be 80:1, and then culturing wild cryptococcus terrestris BSLL1-1, wherein the COD degradation rate in the wastewater is up to 99.92%; the method is characterized in that white spirit production wastewater and pure water (1: 1) are used as fermentation culture media, fructose is used as a carbon source, peptone is used as a nitrogen source, wild cryptococcus terreus BSLL1-1 is cultured after the carbon-nitrogen ratio is adjusted to be 20:1, and the COD degradation rate in the wastewater reaches 76.21%, so that cryptococcus terreus BSLL1-1 has a good application prospect in wastewater treatment, and cryptococcus terreus BSLL1-1 is simple to prepare, easy to store and convenient to use, and is suitable for industrial production and market popularization and application.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but the present invention is not limited thereto, and the methods in the examples are carried out by conventional methods unless otherwise specified, and reagents and strains used therein are, for example, conventional commercially available reagents or strains or reagents prepared by conventional methods unless otherwise specified;
in the embodiment, a potassium dichromate method is adopted to determine the content of COD, and then an ultraviolet spectrophotometer is utilized to determine the absorbance and calculate the content of COD; the method for measuring total phosphorus and ammonia nitrogen also uses an ultraviolet spectrophotometry;
the calculation formula is as follows:
（1）COD（mg/L）=[（λ620-0λ620）+0.0017]0.0003 Xdilution factor
(2) Total phosphorus (μ g/mL) =
(3) Ammonia nitrogen (mg/mL) =
(4) Removal rate (%) = (content of COD, total phosphorus or ammonia nitrogen in original solution-content of COD, total phosphorus or ammonia nitrogen in bacterial liquid after thallus culture)/content of COD, total phosphorus or ammonia nitrogen in original solution is multiplied by 100%
Wherein λ620: absorbance of the wastewater sample at 620 nm; 0 lambda620: absorbance of the blank solution at 620 nm; lambda [ alpha ]700: absorbance of a wastewater sample at 700 nm; 0 lambda700: absorbance of blank solution at 700nm；λ420: absorbance of the wastewater sample at 420 nm; 0 lambda420 : absorbance of blank solution at 420nm, V: and (4) determining the volume of the wastewater sample.
Example 1: activation culture of cryptococcus terrestris BSLL1-1
YPD slant medium (g/L): yeast extract powder 10, peptone 20, glucose 20 and agar powder 20; YPD seed Medium (g/L): yeast extract powder 10, peptone 20, and glucose 20; firstly, the cryptococcus terrestris BSLL1-1 frozen in glycerol is placed on ice to be unfrozen and then inoculated into a YPD slant culture medium by a plate streaking method, then the cryptococcus terrestris BSLL is cultured for 2 days by using an incubator at the temperature of 28 ℃, a single colony is picked and inoculated into 10mL of YPD seed culture medium, and the YPD seed culture medium is subjected to shake cultivation for 24 hours at the temperature of 28 ℃ and at the speed of 180rpm, so that a seed solution of the cryptococcus terrestris BSLL1-1 is prepared.
Example 2: experiment for degrading COD, ammonia nitrogen and total phosphorus in brewing wastewater by using wild cryptococcus terrestris BSLL1-1
1. In the embodiment, COD in the kaoliang spirit production wastewater is 18340mg/L, total phosphorus is 105.8mg/L and ammonia nitrogen is 44mg/L, and COD in the white spirit production wastewater is 98446mg/L, total phosphorus is 130.68mg/L and ammonia nitrogen is 0.132 mg/L;
fermentation medium: (1) naturally settling the sorghum liquor production wastewater for 24 hours, selecting a supernatant, sterilizing and directly using the supernatant as a fermentation medium; (2) naturally settling the white spirit production wastewater for 24 hours, selecting a supernatant, sterilizing and directly using the supernatant as a fermentation medium; (3) adding pure water with the same volume as the supernatant of settled sorghum liquor production wastewater for 24 hours to sterilize and taking the supernatant as a fermentation medium; (4) adding pure water with the same volume to the supernatant after the white spirit production wastewater is settled for 24 hours for sterilization to serve as a fermentation culture medium;
2. selecting 4 tubes of activated seed liquid of wild cryptococcus oxytetracycline BSLL1-1, centrifugally collecting thalli, washing the thalli for 2-3 times by using a proper amount of sterile water, respectively adding 4 types of 10mL fermentation culture media for suspension, then inoculating the thalli into 50mL corresponding fermentation culture media according to 15% inoculation amount, carrying out shake cultivation at 28 ℃ and 180rpm for 6 days, centrifugally collecting supernatant to measure COD (chemical oxygen demand), total phosphorus and ammonia nitrogen content of the four culture liquids, and calculating degradation rate of the four culture liquids;
the results show that: when the supernatant fluid obtained after the sorghum liquor production wastewater is settled for 24 hours is used for culturing wild cryptococcus oxytetracycline BSLL1-1, the removal rate of COD is 75.39%, the removal rate of ammonia nitrogen is 34.09%, and the removal rate of total phosphorus reaches 80.10%; when the culture medium prepared by adding pure water into supernatant fluid obtained after the sorghum liquor production wastewater is settled is used for culturing wild cryptococcus oxytetracycline BSLL1-1, the COD removal rate is 68.87%, the ammonia nitrogen removal rate is 40.91%, and the total phosphorus removal rate reaches 56.05%;
when the wild cryptococcus terrestris BSLL1-1 is cultured by using the supernatant obtained after the white spirit production wastewater is settled for 24 hours, the removal rate of COD is 31.16%, the removal rate of ammonia nitrogen is 84.09%, and the removal rate of total phosphorus reaches 83.86%; when the diluted wastewater is used for culturing the wild cryptococcus terrestris BSLL1-1, the COD removal rate is 37.96%, the ammonia nitrogen removal rate is 27.27%, and the total phosphorus removal rate reaches 37.96%.
Example 3: strain domestication of cryptococcus terreus BSLL1-1 and treatment of wastewater
1. Preparing an acclimatization culture medium:
(1) naturally settling the sorghum liquor production wastewater for 24 hours, taking supernatant, sterilizing and directly using the supernatant as a domestication culture medium; (2) naturally settling the white spirit production wastewater for 24 hours, selecting a supernatant, sterilizing, and directly using the supernatant as an acclimation culture medium;
2. strain acclimatization process
Respectively inoculating activated 4 tubes of wild cryptococcus soil palustris BSLL1-1 into the domestication culture media, carrying out shake cultivation at 28 ℃ and 180rpm for 48h, then centrifuging at 5000rpm and 4 ℃ for 5min, discarding the supernatant, then respectively adding 10mL of corresponding domestication culture medium to suspend the thalli, carrying out shake cultivation at 28 ℃ and 180rpm for 48h, repeatedly circulating the process for 5 times to obtain 2 strains prepared by using different domestication culture media, respectively inoculating the 2 domestication strains into corresponding fermentation culture media according to 15% of inoculation amount, carrying out shake cultivation at 28 ℃ and 180rpm for 6 days, centrifugally collecting the supernatant to determine the COD, the total phosphorus and the ammonia nitrogen content of the bacteria liquid, and calculating the degradation rate.
The results show that: the domesticated strain obtained after the sorghum liquor production wastewater is used as the domestication culture medium is inoculated in the original sorghum liquor production wastewater again, the removal rate of COD reaches 78.66%, the removal rate of ammonia nitrogen reaches 56.82%, and the removal rate of total phosphorus reaches 85.56%; the domesticated strain obtained after the white spirit production wastewater is used as the domesticated culture medium is inoculated in the white spirit wastewater stock solution again, the removal rate of COD is 78.66%, the removal rate of ammonia nitrogen is 87.88%, the removal rate of total phosphorus is 87.83%, and the removal rates of total phosphorus and ammonia nitrogen are higher than that obtained by culturing wild cryptococcus terreus BSLL1-1 in the white spirit wastewater stock solution.
Example 4: treatment effect of cryptococcus terrestris BSLL1-1 on brewing wastewater with carbon-nitrogen ratio adjusted
Taking kaoliang spirit production wastewater as a fermentation culture medium, using glucose as a carbon source and yeast powder as a nitrogen source, respectively adjusting the carbon-nitrogen ratio to be 20:1, 40:1, 60:1, 80:1 and 100:1, then sterilizing at 115 ℃ for 20min, respectively inoculating seed liquid of cryptococcus terreus BSLL1-1 into 5 fermentation culture media according to 15 percent of inoculation amount, measuring the value of COD after shake cultivation for 144h at 28 ℃ and 180rpm, and calculating the removal rate; the result shows that when the carbon-nitrogen ratio is 80:1, the COD degradation rate is as high as 99.92 percent;
using white spirit wastewater and pure water (volume ratio is 1: 1) as a fermentation medium, using fructose as a carbon source and peptone as a nitrogen source, respectively adjusting the carbon-nitrogen ratio to be 20:1, 40:1, 60:1, 80:1 and 100:1, then sterilizing at 115 ℃ for 20min, respectively inoculating seed liquid of cryptococcus terreus BSLL1-1 into 5 fermentation media according to 15% inoculation amount, performing shake culture at 28 ℃ and 180rpm for 144h, measuring the content of COD, and calculating the removal rate; the results show that the COD degradation rate is as high as 81.87% when the carbon-nitrogen ratio is 20: 1.
The above embodiments describe the main features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the present invention, and that various changes and modifications may be made without departing from the scope of the principles of the present invention, and the invention is intended to be covered by the appended claims.
Claims (1)
1. Cryptococcus oxytetracycline (Cryptococcus humicolus) BSLL1-1 for reducing COD and ammonia in brewing wastewaterNitrogen and total phosphorus content.
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| CN114045294A (en) * | 2021-11-22 | 2022-02-15 | 昆明理工大学 | Lipid transport protein gene and application thereof |
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