CN110104921B - Method for improving dewatering performance of waste activated sludge by adding microbial fermentation liquor - Google Patents
Method for improving dewatering performance of waste activated sludge by adding microbial fermentation liquor Download PDFInfo
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- C02F11/00—Treatment of sludge; Devices therefor
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- C—CHEMISTRY; METALLURGY
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention discloses a method for improving the dehydration performance of waste activated sludge by adding microbial fermentation liquor, wherein the microorganisms select aspergillus niger, pseudomonas aeruginosa, streptococcus thermophilus and rhodococcus, the fermentation culture solution is prepared by waste molasses diluent and waste activated sludge, four microorganisms are inoculated to respective culture media for culture and then are inoculated into the fermentation culture solution in proportion, the fermentation is carried out for 2-5d under the conditions of 25-35 ℃ and 180r/min with 160-; the composite microbial fermentation liquor has good adaptability to temperature, pH value and the like, the flocculation capacity is strong, the dehydration effect is obviously improved, the method is simple and convenient, the cost is low, and the sludge subjected to dehydration treatment can be used as a culture medium or a microbial fertilizer, so that the resource utilization of the sludge is promoted.
Description
Technical Field
The invention relates to the technical field of environmental engineering and sludge treatment, in particular to a method for improving the dehydration performance of waste activated sludge by adding microbial fermentation liquor.
Background
In recent years, with the acceleration of urbanization progress and the improvement of environmental quality standards in China, the amount of sewage to be treated is increased rapidly, so that various sewage treatment processes such as oxidation ditches, biological filters and oxidation ponds are widely applied at the present stage, and finally, a large amount of another pollutant (sludge) is generated in sewage treatment.
At present, the treatment and disposal methods of sludge mainly comprise sanitary landfill, incineration, fertilizer manufacturing, building material manufacturing and the like, and the methods all require that the sludge is subjected to dehydration and drying treatment, namely, the water content in the sludge is reduced to below 50 percent, so that the sludge can be stabilized and reduced for further disposal.
The existing sludge dewatering generally adopts a physical, chemical or biological method to condition the sludge so as to improve the sludge dewatering effect, and is an effective way to reduce the sludge volume and reduce the disposal and transportation cost. At present, means such as acid treatment, ultrasound, microwave, electrolysis, cold melting and the like are applied to sludge dewatering, but the methods have high treatment cost and limit large-scale popularization in the field of water treatment. In addition, the method for conditioning the sludge by using organic, inorganic and microbial flocculants is also a common method for sludge dewatering treatment at present, but the application of the organic and inorganic flocculants to sludge conditioning has high cost and may generate secondary pollution. Although the microbial flocculant can reduce the pollution problem caused by the flocculant, the preparation process of the flocculant is complicated, and the effect of conditioning and dewatering sludge is general. Under the condition of the sludge dewatering process of the sewage plant in China at present, the water content of the sludge can only be reduced to 70% -80%, and many sewage plants do not dewater the sludge, the water content of the sludge is often over 90%, and the requirements of treatment such as sanitary landfill and the like cannot be met. On the other hand, the water content in the dewatered sludge is mainly the intracellular water content in the sludge, and the conventional dewatering method is not suitable for removing the water content.
Disclosure of Invention
The invention provides a method for conditioning sludge by adding microbial fermentation liquor, waste activated sludge prepared by the method and application of the waste activated sludge, aims at the current situations of poor sludge dewatering performance and high subsequent treatment and disposal cost of sludge cakes of urban and industrial sewage treatment plants, overcomes the economic limitation of sludge treatment by conventional physical and chemical means, improves the sludge dewatering performance, has low cost, avoids the problems of secondary pollution and the like, and achieves the purposes of sludge reduction and resource disposal.
In order to achieve the aim, the invention provides a method for improving the dehydration performance of waste activated sludge by adding microbial fermentation liquor, which comprises the following steps:
(1) micro-meterBiological culture: selecting Aspergillus niger, Pseudomonas aeruginosa, Streptococcus thermophilus and Rhodococcus, inoculating the microorganism into respective culture medium, and culturing until the thallus number reaches 108-109cfu/ml；
(2) Preparing waste molasses fermentation culture solution: adding industrial sugar-making byproduct waste molasses into a dilution tank, adding water, stirring and diluting to 30-40% to prepare waste molasses fermentation culture solution;
(3) preparing a composite microbial fermentation liquid: inoculating four microorganisms into the waste molasses fermentation culture solution according to a proportion, and fermenting for 2-5 days at the temperature of 25-35 ℃ and under the condition of 160-;
(4) sludge dewatering treatment: compounding microbial fermentation liquor according to the volume ratio: the activated sludge is 1: 8-12, directly adding the composite microbial fermentation liquor into the waste activated sludge, stirring and reacting for 4 hours, and then dehydrating, wherein the water content of the sludge is reduced to below 50%.
Preferably, the waste molasses fermentation culture solution also comprises waste activated sludge, and the waste activated sludge is prepared by mixing the waste molasses diluent: waste activated sludge is mixed at a ratio of 4-8:1 to prepare waste molasses fermentation culture solution. By mixing waste activated sludge into the waste molasses diluent, the environmental adaptability of microorganisms is improved and the subsequent sludge dewatering treatment capability is promoted while fermentation is carried out.
Preferably, the ratio by volume of aspergillus niger: pseudomonas aeruginosa: streptococcus thermophilus: the rhodococcus is 2-3: 1: 1: 1 inoculating each microorganism into the waste molasses fermentation culture solution.
Preferably, the Aspergillus niger culture comprises the following steps:
1) separation and purification of aspergillus niger: the Aspergillus niger spore suspension was inoculated with an inoculating loop into a small tube slant and then incubated in an incubator at 37 ℃ for 24 hours. And selecting bacterial colonies in a culture dish for amplification culture by a plate marking method. Repeating the steps, separating and purifying for 3-5 times to obtain Aspergillus niger with single colony, inoculating onto slant culture medium, and storing in refrigerator at 4 deg.C;
2) preparing a liquid culture medium: the liquid culture medium is prepared from 40g/L glucose, 20g/L peptone and 10g/L yeast powder, and is sterilized at 121 deg.C for 30min under high pressure;
3) inoculating and culturing aspergillus niger: inoculating the purified Aspergillus niger strain to the liquid culture medium from the slant culture medium, fermenting at 30 deg.C by constant temperature shaking table, and shake culturing at 180r/min until the thallus number reaches 108-109More than cfu/ml.
Preferably, the pseudomonas aeruginosa is cultured by adopting an LB liquid culture medium, the pseudomonas aeruginosa is inoculated to the LB liquid culture medium, and the pseudomonas aeruginosa is subjected to shaking culture at the temperature of 28-32 ℃ and at the speed of 160r/min until the thallus number reaches 108-109cfu/ml; the LB liquid culture medium comprises the following components in percentage by weight: 1000ml of water, 10g of yeast powder, 8g of peptone, 5g of sodium chloride and 2g of monopotassium phosphate, and the pH value is 7.0.
Preferably, the Streptococcus thermophilus is cultured by inoculating activated Streptococcus thermophilus into liquid culture medium, and culturing at 35 deg.C until the thallus number reaches 108-109cfu/ml; the liquid culture medium comprises 7.5g of yeast powder, 7.5g of peptone, 8g of glucose, 2g of monopotassium phosphate, 100ml of tomato juice, 900ml of water and pH6.8.
Preferably, the rhodococcus is cultured by adopting an LB liquid culture medium, the activated rhodococcus is inoculated to the LB liquid culture medium, and the rhodococcus is subjected to shaking culture at the temperature of 30-35 ℃ and at the speed of 150r/min until the thallus number reaches 108-109cfu/ml; the LB liquid culture medium comprises the following components in percentage by weight: 1000ml of water, 5g of yeast extract, 10g of peptone and 5g of sodium chloride, and the pH value is 7.0.
Preferably, the water content of the waste activated sludge is more than 90%.
In addition, the invention also provides waste activated sludge, and the water content of the waste activated sludge prepared by the method is reduced to below 50%, so that convenience is brought to further recycling treatment of the waste activated sludge.
Moreover, the invention also provides an application of the waste activated sludge prepared by the method, and as the compound microorganism fermentation liquor is rich in beneficial microorganisms and mineral substances, the microorganisms decompose harmful and difficultly decomposed substances in the waste activated sludge, the heavy metal content is reduced, the nutrient content of the waste activated sludge is increased, the physicochemical property of the sludge is improved, and the obtained sludge can be applied to culture substrates or microbial fertilizers.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the invention, Aspergillus niger, Pseudomonas aeruginosa, Streptococcus thermophilus and Rhodococcus composite microorganisms are adopted for fermentation culture, so that on one hand, the fermentation liquor has good adaptability to temperature, pH value and the like, high stability and strong flocculation capability; on the other hand, the selection and reasonable compatibility of the microorganisms degrade and convert harmful and difficultly decomposed substances in the sludge, destroy the colloid components of the sludge and promote the conversion of the combined water to the free water.
(2) The fermentation culture solution adopts waste honey fermentation culture solution, and the waste honey is rich in carbon source, mineral matter, crude protein, vitamin and other nutrient substances, is suitable for fermentation culture of microorganisms, and has low cost and strong flocculation capacity of fermentation liquor.
(3) The invention improves the environmental adaptability of the microbial sludge and promotes the subsequent sludge dehydration treatment capability at the same time of fermentation by mixing the waste activated sludge into the waste molasses diluent.
(4) The fermentation liquor is simple to prepare, is directly added into the waste activated sludge, does not need to prepare a microbial flocculant first, simplifies the sludge dewatering process and reduces the cost.
(5) The sludge treated by the composite microorganism fermentation liquor contains various beneficial bacteria, has higher contents of organic nutrients and mineral substances, improves the physical and chemical properties of the sludge, and is suitable for being used as a microorganism fertilizer or a culture medium.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
The invention relates to a method for improving the dehydration performance of residual activated sludge by adding microbial fermentation liquor, which is implemented specifically, a sludge sample is taken from a concentration tank of a certain sewage treatment plant in Hangzhou Zhejiang, and the basic characteristics of the sludge to be tested are shown in Table 1.
TABLE 1 basic characteristics of the sludge samples tested
| pH | CST(s) | Water content (%) |
| 7.17 | 400 | 90 |
Example 1:
(1) cultivation of microorganisms
1.1 culture of Aspergillus niger
1) Separation and purification of aspergillus niger: the Aspergillus niger spore suspension is sequentially diluted to different concentration gradients (10) on a sterile operating platform2～108) The spread plate method comprises inoculating Aspergillus niger on Sabouraud Dextrose Agar (SDA) and steam sterilizing at 121 deg.C for 30 min. After inoculation, the culture dish is inverted and is cultured for 48 hours in a biochemical incubator at the constant temperature of 28 ℃. And (3) selecting bacterial colonies in a glass culture dish by using a plate scribing method, repeating the step for 4 times, selecting a small amount of bacterial lawn of a single bacterial colony, coating the bacterial lawn on a glass slide to carry out microscope individual morphology observation, and carrying out comprehensive analysis by combining the morphological characteristics of the bacterial colonies. If not, the separation and purification are carried out continuously by using a plate marking method. If the culture medium is pure, inoculating the culture medium on a slant culture medium, and storing the culture medium in a refrigerator at 4 ℃;
2) preparing a liquid culture medium: adding glucose 40g/L, peptone 20g/L, and yeast powder 10g/L into distilled water, stirring, and sterilizing with high pressure steam at 120 deg.C for 30 min;
3) selecting purified Aspergillus niger strain from slant culture medium, inoculating into sterilized liquid culture medium, and shake culturing at 30 deg.C until thallus number reaches 108-109cfu/ml。
1.2 culture of Pseudomonas aeruginosa
1) Preparing an LB liquid culture medium, preparing the LB liquid culture medium according to 1000ml of water, 10g of yeast powder, 8g of peptone, 5g of sodium chloride and 2g of monopotassium phosphate, adjusting the pH to 7.0, and sterilizing for 30min at 120 ℃ by high-pressure steam for later use;
2) inoculating activated pseudomonas aeruginosa to an LB liquid culture medium, and carrying out shake culture at the temperature of 28-32 ℃ and under the condition of 160r/min until the thallus number reaches 108-109cfu/ml。
1.3 culture of Streptococcus thermophilus
1) Preparing a liquid culture medium, preparing the liquid culture medium according to the formula of 7.5g of yeast powder, 7.5g of peptone, 8g of glucose, 2g of monopotassium phosphate, 100ml of tomato juice and 900ml of water, adjusting the pH value to 6.8, and sterilizing for 30min at 120 ℃ by high-pressure steam for later use;
2) inoculating activated Streptococcus thermophilus into liquid culture medium, and culturing at 35 deg.C until thallus number reaches 108-109cfu/ml。
1.4 Rhodococcus culture
1) Preparing an LB liquid culture medium, preparing the LB liquid culture medium according to 1000ml of water, 5g of yeast extract, 10g of peptone and 5g of sodium chloride, adjusting the pH to 7.0, and sterilizing for 30min at 120 ℃ by high-pressure steam for later use;
2) inoculating activated Rhodococcus into LB liquid culture medium, and shake culturing at 30-35 deg.C and 150r/min until thallus number reaches 108-109cfu/ml。
(2) Preparing waste molasses fermentation culture solution: adding industrial sugar-making byproduct molasses into a dilution tank, adding water, stirring and diluting to 30% to obtain fermentation culture solution, and sterilizing the fermentation culture solution at 120 deg.C under high pressure steam for 30 min.
(3) Preparing a composite microbial fermentation liquid: aspergillus niger in volume ratio: pseudomonas aeruginosa: streptococcus thermophilus: the rhodococcus is 2: 1: 1: 1 inoculating each microorganism into the waste molasses fermentation culture solution, and fermenting for 5d under the conditions of 25 ℃ and 160-.
(4) Sludge dewatering treatment: compounding microbial fermentation liquor according to the volume ratio: the activated sludge is 1: and 8, directly putting the composite microbial fermentation liquor into a sludge sample to be detected, stirring for a period of time, standing, and detecting CST once every hour. And after 4h of reaction, transferring the sludge sample to a sludge specific resistance measuring device, filtering under the vacuum degree condition of 0-0.1 MPa, and recording the filtering time t and the corresponding filtrate volume V. And (3) selecting Specific Resistance (SRF) of the sludge and water content to represent the sludge dewatering effect, and measuring the water content by adopting a gravimetric method. The Specific resistance of Sludge (SRF) represents the resistance of unit mass of sludge on unit filtration area when filtering under constant pressure, and the larger the Specific resistance of sludge, the poorer the filtration performance, and the Specific resistance of sludge is calculated by formula (1):
wherein P is the filtration pressure, N/m2(ii) a A is the filtration area, m2(ii) a Mu is the viscosity of the filtrate, N.s/m2(ii) a C is the weight of dry solids retained on the filter medium per unit volume of filtrate, kg/m3，1/C＝Ci/(100-Ci)-Cf/(100-Cf)；CiThe water content of the raw mud is percent; cfWater content of the mud cake after suction filtration is percent; b is the slope of a straight line shown by a filtering equation t/V ═ bV + a, t is filtering time, and s; v is the volume of filtrate, m3。
The sludge is conditioned by adding the composite microbial fermentation liquid, and the dehydration performance of the sludge can be obviously improved. The specific resistance of the sludge is slightly increased, and the water content is reduced from 90 percent of the original sludge to 49.6 percent of the conditioned sludge.
Example 2
(1) The microorganism was cultured in accordance with example 1.
(2) Preparing waste molasses fermentation culture solution: taking industrial sugar-making byproduct waste molasses, adding the industrial sugar-making byproduct waste molasses into a dilution tank, adding water, stirring and diluting to 40%, and mixing the waste molasses diluent according to the volume ratio: mixing waste activated sludge at a ratio of 4-8:1 to obtain waste molasses fermentation culture solution, and sterilizing the fermentation culture solution at 120 deg.C under high pressure steam for 30 min.
(3) Preparing a composite microbial fermentation liquid: aspergillus niger in volume ratio: pseudomonas aeruginosa: streptococcus thermophilus: the rhodococcus is 3: 1: 1: 1 inoculating each microorganism into the waste molasses fermentation culture solution, and fermenting for 2d under the conditions of 35 ℃ and 160-.
(4) Sludge dewatering treatment: compounding microbial fermentation liquor according to the volume ratio: the activated sludge is 1: 12, directly putting the composite microbial fermentation liquor into a sludge sample to be detected, and dehydrating after reacting for 4 hours.
Through the dehydration treatment, the water content of the waste activated sludge is reduced from 90 percent of the original sludge to 48.5 percent of the conditioned waste activated sludge.
The embodiments described in this specification are merely illustrative of implementations of the inventive concept and the scope of the present invention should not be considered limited to the specific forms set forth in the embodiments but rather the scope of the present invention is intended to include equivalents thereof as may be devised by those skilled in the art based on the teachings of the present invention.
Claims (5)
1. A method for improving the dehydration performance of waste activated sludge by adding microorganism fermentation liquor is characterized by comprising the following steps:
1) and (3) culturing microorganisms: selecting Aspergillus niger, Pseudomonas aeruginosa, Streptococcus thermophilus and Rhodococcus, inoculating the microorganism into respective culture medium, and culturing until the thallus number reaches 108-109cfu/ml；
2) Preparing waste molasses fermentation culture solution: adding industrial sugar-making byproduct waste molasses into a dilution tank, adding water, stirring and diluting to 30-40% to obtain waste molasses fermentation culture solution, and sterilizing the fermentation culture solution at 120 deg.C under high pressure steam for 30 min;
3) preparing a composite microbial fermentation liquid: inoculating four microorganisms into the waste molasses fermentation culture solution according to a proportion, and fermenting for 2-5 days at the temperature of 25-35 ℃ and under the condition of 160-;
4) sludge dewatering treatment: compounding microbial fermentation liquor according to the volume ratio: the waste activated sludge is 1: 8-12, directly adding the compound microorganism fermentation liquor into the waste activated sludge, stirring and reacting for 4 hours, then dehydrating, reducing the water content of the sludge to below 50%, and applying the treated waste activated sludge to a culture substrate or a microorganism fertilizer;
the waste molasses fermentation culture solution also comprises waste activated sludge, and the waste molasses diluent is prepared from the following components in percentage by volume: mixing waste activated sludge at a ratio of 4-8:1 to prepare waste molasses fermentation culture solution;
the step 3) is as follows according to volume ratio of Aspergillus niger: pseudomonas aeruginosa: streptococcus thermophilus: the rhodococcus is 2-3: 1: 1: 1, inoculating each microorganism into waste molasses fermentation culture solution;
the water content of the waste activated sludge is 90%.
2. The method for improving the dewatering performance of waste activated sludge by adding microorganism fermentation broth as claimed in claim 1, wherein the culture of aspergillus niger comprises the following steps:
1) separation and purification of aspergillus niger: inoculating Aspergillus niger spore suspension to a small test tube slant by using an inoculating loop, then culturing in an incubator at 37 ℃ for 24 hours, selecting bacterial colonies in a culture dish by using a plate-scribing method for amplification culture, repeating the steps, separating and purifying for 3-5 times to obtain single bacterial colony Aspergillus niger, inoculating to a slant culture medium, and storing in a refrigerator at 4 ℃;
2) preparing a liquid culture medium: the liquid culture medium is prepared from 40g/L glucose, 20g/L peptone and 10g/L yeast powder, and is sterilized at 121 deg.C for 30min under high pressure;
3) inoculating and culturing aspergillus niger: inoculating the purified Aspergillus niger strain to the liquid culture medium from the slant culture medium, fermenting at 30 deg.C by constant temperature shaking table, and shake culturing at 180r/min until the thallus number reaches 108-109cfu/ml。
3. The method for improving the dewatering performance of waste activated sludge by adding microbial fermentation broth according to claim 1, wherein the Streptococcus thermophilus is cultured by inoculating activated Streptococcus thermophilus into a liquid culture medium and culturing at 35 ℃ until the cell number reaches 108-109cfu/ml; the liquid culture medium comprises 7.5g of yeast powder, 7.5g of peptone, 8g of glucose, 2g of monopotassium phosphate, 100ml of tomato juice, 900ml of water and pH6.8.
4. The method of claim 1A method for improving the dewatering performance of waste activated sludge by adding microbial fermentation liquor is characterized in that rhodococcus is cultured by adopting an LB liquid culture medium, the activated rhodococcus is inoculated to the LB liquid culture medium, and the rhodococcus is subjected to oscillation culture at the temperature of 30-35 ℃ and the speed of 150r/min until the thallus number reaches 108-109cfu/ml; the LB liquid culture medium comprises the following components in percentage by weight: 1000ml of water, 5g of yeast extract, 10g of peptone and 5g of sodium chloride, and the pH value is 7.0.
5. The waste activated sludge prepared by the method for improving the dewatering performance of the waste activated sludge by adding the microbial fermentation broth according to any one of claims 1 to 4, wherein the water content of the prepared waste activated sludge is below 50%.
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| CN101948183A (en) * | 2010-08-27 | 2011-01-19 | 暨南大学 | Microbial flocculant, preparation method and use thereof |
| CN102399694A (en) * | 2011-11-08 | 2012-04-04 | 江苏商达水务有限公司 | Composite microbial inoculum for improving settling performance of activated sludge and manufacture method of composite microbial inoculum |
| CN102719482A (en) * | 2012-02-28 | 2012-10-10 | 湖南大学 | Method for producing microbial flocculant by utilizing culture wastewater and residual activated sludge |
| CN105217805A (en) * | 2015-11-09 | 2016-01-06 | 成都信息工程大学 | A kind of microbial flocculant, preparation method and application thereof |
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| EP3191415A1 (en) * | 2014-09-09 | 2017-07-19 | Novozymes A/S | Methods for enhancing the dewaterability of sludge with enzyme treatment |
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| CN101948183A (en) * | 2010-08-27 | 2011-01-19 | 暨南大学 | Microbial flocculant, preparation method and use thereof |
| CN102399694A (en) * | 2011-11-08 | 2012-04-04 | 江苏商达水务有限公司 | Composite microbial inoculum for improving settling performance of activated sludge and manufacture method of composite microbial inoculum |
| CN102719482A (en) * | 2012-02-28 | 2012-10-10 | 湖南大学 | Method for producing microbial flocculant by utilizing culture wastewater and residual activated sludge |
| CN105217805A (en) * | 2015-11-09 | 2016-01-06 | 成都信息工程大学 | A kind of microbial flocculant, preparation method and application thereof |
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