CN106085923B - A kind of preparation method and application of bacillus amyloliquefaciens and its biological flocculant - Google Patents
A kind of preparation method and application of bacillus amyloliquefaciens and its biological flocculant Download PDFInfo
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- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- 238000005189 flocculation Methods 0.000 claims abstract description 13
- 230000016615 flocculation Effects 0.000 claims abstract description 13
- 239000002351 wastewater Substances 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 4
- 230000000813 microbial effect Effects 0.000 claims abstract 2
- 238000004321 preservation Methods 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 238000009395 breeding Methods 0.000 claims 1
- 230000001488 breeding effect Effects 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000012879 subculture medium Substances 0.000 claims 1
- 239000010865 sewage Substances 0.000 abstract description 6
- 230000003311 flocculating effect Effects 0.000 abstract description 5
- 239000000725 suspension Substances 0.000 abstract description 5
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 230000007423 decrease Effects 0.000 abstract 1
- 241000282898 Sus scrofa Species 0.000 description 10
- 230000001376 precipitating effect Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000008394 flocculating agent Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention discloses the preparation method and application of a kind of bacillus amyloliquefaciens and its biological flocculant, the microbial preservation number of the bacillus amyloliquefaciens FD-14 (Bacillus amyloliquefaciens FD-14) is CCTCC NO:M2016255, its fermentation liquid is the biological flocculant, it can be used cooperatively directly or with flocculation aid, for handling animal farm wastewater.Compared with prior art, it can be effectively reduced sewage of farm concentration of suspension using the biological flocculant that the bacillus amyloliquefaciens produce, concentration of suspension is set to decline 76.7%, with significant flocculating effect, meanwhile flocculant preparation method of the invention is simple, effect is good, it is at low cost, any environmental pollution is not generated.
Description
Technical field
The present invention relates to the technical field of sewage treatment more particularly to a kind of bacillus amyloliquefaciens and its lifes
The preparation method and application of object flocculant.
Background technique
The high speed development of China's large-scale cultivation industry, brings huge environmental pressure, and wherein wastewater from pig farm is main
Pollution source, wastewater from pig farm contain high concentration suspended matter and ammonia nitrogen, are a kind of organic sewages difficult to deal with.It mainly adopts both at home and abroad
It is handled with anaerobic-aerobic technical method, the organic contamination although Anaerobic Treatment can largely degrade in sewage
Object, still, anaerobic effluent concentration of suspension are still very high, and especially ammonia nitrogen is not removed substantially.It needs to detest wastewater from pig farm
Oxygen digestive juice is further processed, using it is most be SBR processing technique.But the suspended matter of high concentration would generally increase microorganism
Load and impact load are handled, causes wastewater treatment efficiency bad.In consideration of it, the flocculation technique of oil removal is efficiently gone gradually to draw
Play the attention of people.Chemical floc is widely used due to efficient, cheap, but can be endangered human health and be increased ring
Border risk.In contrast, biological flocculant has many advantages, such as efficient, safe and nontoxic, biodegradable, without secondary pollution, therefore
The research in relation to biological flocculant is increasingly subject to favor in recent years.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of bacillus amyloliquefaciens and its biology waddings
The preparation method and application of solidifying agent, are particularly suitable for the flocculated new flocculant of sewage of farm to provide one kind, feeding to reduce
Grow wastewater suspended substance.
The present invention is achieved by the following technical solutions:
The present invention provides a kind of bacillus amyloliquefaciens FD-14, Bacillus amyloliquefaciens FD-14,
The bacterium is preserved in China typical culture collection center (abbreviation CCTCC on May 9th, 2016；Address: Wuhan, China, Wuhan
University；Postcode: 430072), deposit number is CCTCC NO:M2016255.
The bacterium is to screen to obtain from the sludge in pig farm precipitating pond, and bacterium colony is approximate circle, and edge is irregular, table
There are protuberance, White-opalescent in face fold, center.Gram-positive, rod-shaped, both ends blunt circle has gemma.It is biological with generating
The ability of flocculant, in the flocculant using its production, polyoses content is up to 65% or more, protein content only 2%, for typical case
Polysaccharide biological flocculant, 16SrDNA sequence is as shown in SEQ ID NO:1.
The present invention also provides a kind of preparation methods of the biological flocculant of above-mentioned bacillus amyloliquefaciens FD-14, including
Following steps:
(1) the bacillus amyloliquefaciens FD-14 is inoculated in seed culture medium and is expanded culture, obtain seed
Liquid；
(2) seed liquor of step (1) is seeded in fermentation medium, carries out fermented and cultured, obtaining has flocculation activity
Fermentation liquid, the as described biological flocculant.
Further, by weight, the formula of the seed culture medium includes: beef extract 0.3%~0.5%, peptone
1%, sodium chloride 0.5%~0.8%, pH is 6.5~7.0；The formula of the fermentation medium includes: starch 2%, yeast powder
0.5% and beef extract 0.2%, magnesium sulfate 0.2%, sodium chloride 1%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.2%, pH be
6.6。
Further, in the step (1), the bacterial strain concentration in seed liquor is 1 × 108A/m3More than.
Further, in the step (2), the inoculum concentration of seed liquor is that every 100mL fermentation medium is inoculated with 2mL seed
Liquid.
Further, in the step (2), the temperature of fermented and cultured is 33 DEG C, pH 6.6, revolving speed 150r/min, when
Between be 24-42h.
The present invention also provides a kind of biological flocculant, the biological flocculant is prepared using the above method.
The present invention also provides application of the above-mentioned biological flocculant in processing farm's aquaculture wastewater.
Further, when using above-mentioned biological flocculant processing hoggery sewerage, flocculation aid can first be added, stirring is mixed
After even, add biological flocculant processing, calcium chloride can be selected in the flocculation aid, but not limited to this, it is other can be with institute
Stating the chemical reagent that biological flocculant is used cooperatively and improves cohesion and flocculating effect should belong to the protection scope of the application.
The present invention has the advantage that the present invention provides a kind of bacillus amyloliquefaciens and its biologies compared with prior art
The preparation method of flocculant can be effectively reduced hoggery sewerage suspended matter using the biological flocculant that this method prepares
Concentration, concentration of suspension have dropped 76.7%, have significant flocculating effect.Flocculant preparation method of the invention is simple,
Effect is good, at low cost, does not generate any environmental pollution.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
1, material
The present embodiment method therefor is known to those skilled in the art the conventional method of dawn unless otherwise instructed, used
The materials such as reagent be commercially available products unless otherwise instructed.
2, method
2.1 screening bacillus amyloliquefaciens FD-14 bacterial strains
2.1.1 primary dcreening operation: the sludge in the pig farm 2g precipitating pond and the sewage sample in the pig farm 2mL precipitating pond are taken, is separately added into
Into 98mL Enrichment of bacteria culture medium and fungi enriched medium, respectively at 37 DEG C, 150rpm and 30 DEG C, the oscillation of 150rpm shaking table
Enrichment culture.Enrichment culture liquid is taken, after suitably being diluted with sterile water, 0.1mL is taken to be respectively coated on fungi isolation medium and thin
On bacterium isolation medium, 48h is cultivated under the conditions of enrichment culture temperature, observe colonial morphology, select that surface is smooth and big and band
The bacterium colony of viscosity is isolated and purified using plate streak, is saved backup in 4 DEG C of refrigerators.
2.1.2 secondary screening: the bacterial strain that primary dcreening operation is obtained is linked into 100mL Standard fermentation culture medium, and respectively at corresponding
Screening at a temperature of, take bacterium solution after 150rpm shaking table shaken cultivation 72h.Using bacterium solution to the flocculating rate of wastewater from pig farm as foundation, choosing
The bacterial strain of efficient flocculant is generated out.
2.1.3 result is identified
Cytological Identification result: the bacterial clump is approximate circle, and edge is irregular, surface folding, and there is protuberance in center, white
It is opaque, it is Gram-positive, rod-shaped, both ends blunt circle has gemma.
Molecular biology identification result: through molecular biology identification, 16SrDNA sequence length is 1463pb, is had such as
Nucleotide sequence shown in SEQ ID NO:1, the gene order are registered in GenBank, number of registration are as follows: KU904266, it should
Multiple bacterial strains of bacterium and bacillus have homology, carry out Phylogenetic Analysis to bacterial strain FD-14 through Mega4.0 software,
Wherein the homology with bacillus amyloliquefaciens (KP095106.1) reaches 99%, thus determines that it is bacillus amyloliquefaciens.
2.2 prepare biological flocculant
(1) the bacillus amyloliquefaciens FD-14 is first activated, is then seeded in seed culture medium, at 33 DEG C
Expand culture to bacterial strain concentration up to 1 × 108A/m2The above order of magnitude obtains seed liquor；The formula of the seed culture medium are as follows: press
Poidometer, including beef extract 0.3%~0.5%, peptone 1%, sodium chloride 0.5%~0.8%, pH are 6.5~7.0.
(2) seed liquor of step (1) is seeded in fermentation medium, inoculum concentration is the inoculation of every 100ml fermentation medium
The seed liquor of 2ml, fermentation culture conditions are as follows: 33 DEG C of temperature, pH6.6, cultivate revolving speed 150r/min, cultivate 42h, fermented
Liquid, as biological flocculant；Wherein, the formula of fermentation medium are as follows: by weight, including starch 2%, 0.5% He of yeast powder
Beef extract 0.2%, magnesium sulfate 0.2%, sodium chloride 1%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.2%, pH 6.6.
(3) the biological flocculant ingredient of step (2) is identified, as a result, it has been found that, polyoses content in the biological flocculant
Up to 65% or more, protein content only 2% is typical polysaccharide biological flocculant.
Application of 2.3 biological flocculants in processing pig animal farm wastewater
In the flocculation reaction tank equipped with pig animal farm wastewater, the calcium chloride solution of 0.02% (mass/volume) is first added
As flocculation aid, with 100r/min revolving speed uniform stirring, the above-mentioned bioflocculation prepared of 1% (volume) is then added
Agent continues finally to be stood 30min with 100r/min revolving speed uniform stirring 10min, observed waste water before and after the processing.
3, result
The pig animal farm wastewater of comparison before and after the processing, as a result, it has been found that: sewage obviously becomes to clarify after standing 30min, suspends
Object is deposited to reaction pot bottom.Using the suspended matter of gravimetric detemination hoggery sewerage flocculation front and back, the results show that after flocculation
Concentration of suspension in hoggery sewerage is than reducing 76.7% before flocculation.Illustrate that biological flocculant of the invention can be significant
The suspended matter for removing hoggery sewerage, improves the pollution level of hoggery sewerage, cooperates with flocculation aid, flocculating effect is more
Significantly.
The above are a kind of detailed embodiment and specific operating process of the present invention, are before being with technical solution of the present invention
It puts and is implemented, but protection scope of the present invention is not limited to the above embodiments.
Claims (9)
1. a kind of bacillus amyloliquefaciens FD-14, Bacillus amyloliquefaciens FD-14, which is characterized in that should
The microbial preservation number of bacterium is CCTCC NO:M2016255.
2. a kind of preparation method of biological flocculant, which comprises the following steps:
(1) bacillus amyloliquefaciens FD-14 as described in claim 1 is inoculated in seed culture medium and is expanded culture,
Obtain seed liquor；
(2) seed liquor of step (1) is seeded in fermentation medium, carries out fermented and cultured, obtain the hair with flocculation activity
Zymotic fluid, the as described biological flocculant.
3. a kind of preparation method of biological flocculant according to claim 2, which is characterized in that by weight, described kind
The formula of sub- culture medium includes: beef extract 0.3%~0.5%, peptone 1%, sodium chloride 0.5%~0.8%, and pH is 6.5~
7.0；The formula of the fermentation medium includes: starch 2%, yeast powder 0.5% and beef extract 0.2%, magnesium sulfate 0.2%, chlorine
Change sodium 1%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.2%, pH 6.6.
4. a kind of preparation method of biological flocculant according to claim 2, which is characterized in that in the step (1), kind
Bacterial strain concentration in sub- liquid is 1 × 108A/m3More than.
5. a kind of preparation method of biological flocculant according to claim 2, which is characterized in that in the step (2), kind
The inoculum concentration of sub- liquid is that every 100mL fermentation medium is inoculated with 2mL seed liquor.
6. a kind of preparation method of biological flocculant according to claim 2, which is characterized in that in the step (2), hair
The temperature of ferment culture is 33 DEG C, pH 6.6, revolving speed 150r/min, time 24-42h.
7. a kind of biological flocculant, which is characterized in that the biological flocculant is using such as any the method for claim 2-6
It prepares.
8. a kind of application of biological flocculant as claimed in claim 7 in processing hoggery sewerage.
9. application of the biological flocculant according to claim 8 in processing hoggery sewerage, which is characterized in that first exist
Flocculation aid is added in the breeding wastewater of pig farm, after mixing evenly, adds the biological flocculant.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433284A (en) * | 2011-12-21 | 2012-05-02 | 浙江理工大学 | Preparation method of dedicated microbial flocculant for dairy cattle breeding waste water and strain used in method |
| CN103937695A (en) * | 2013-07-22 | 2014-07-23 | 河海大学 | Composite biological bacterial agent for treating livestock and poultry breeding wastewater and manufacturing method thereof |
| CN105347952A (en) * | 2015-12-02 | 2016-02-24 | 合肥四方磷复肥有限责任公司 | Naturally-flocculent microbial fertilizer and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433284A (en) * | 2011-12-21 | 2012-05-02 | 浙江理工大学 | Preparation method of dedicated microbial flocculant for dairy cattle breeding waste water and strain used in method |
| CN103937695A (en) * | 2013-07-22 | 2014-07-23 | 河海大学 | Composite biological bacterial agent for treating livestock and poultry breeding wastewater and manufacturing method thereof |
| CN105347952A (en) * | 2015-12-02 | 2016-02-24 | 合肥四方磷复肥有限责任公司 | Naturally-flocculent microbial fertilizer and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 一株絮凝剂产生菌TS-1的分离与鉴定;潘素娟等;《安徽农业科学》;20111231;11388-11390 * |
| 一株絮凝剂产生菌的筛选鉴定和培养条件优化;李潭等;《广西大学学报(自然科学版)》;20151031;1305-1313 * |
| 解淀粉芽孢杆菌dhs-28的筛选分离及絮凝活性;郝建安等;《化学工业与工程》;20110131;7-12 * |
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