CN108676763A - A kind of high resistance to antimony Cattell proteus DSHN0704 and its separating screening method and application - Google Patents
A kind of high resistance to antimony Cattell proteus DSHN0704 and its separating screening method and application Download PDFInfo
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Abstract
The present invention relates to a kind of high resistance to antimony Cattell proteus DSHN0704 and its separating screening method and applications, belong to microorganisms technical field.The strain classification of the present invention is named as：Cattell proteus (Proteus cibarius) DSHN0704, has been preserved in China typical culture collection center (CCTCC), deposit number is：CCTCC NO：M2018184, preservation date：On April 8th, 2018；For the 16S rRNA sequences of the Cattell proteus DSHN0704 of the present invention as shown in SEQ ID No.1, the bacterial strain is to shave separation screening in contaminated soil from Lengshuijiang, Hunan tinnery to obtain.The Cattell proteus bacterial strain DSHN0704 bacterial strains of the present invention have higher adsorption capacity to antimony in water body (Sb) ion, and adsorption rate is fast, easy to maintain, environmentally friendly, have good development and application potentiality.
Description
Technical field
The invention belongs to microorganisms technical fields, are related to a kind of Cattell proteus, it is more particularly related to one
The high resistance to antimony Cattell proteus DSHN0704 of kind and its separating screening method and application.
Background technology
Antimony (Sb) has the characteristics that chronic toxicity, carcinogenicity and is in global migration, is global contaminant and priority acccess control
Pollutant.Antimony (Sb) and its compound can enter human body by approach such as food chain and water, not only influence the effect of certain enzymes, also
It can lead to the disease in terms of liver and cardiovascular system, therefore, the World Health Organization, European Union and China are to antimony content in drinking water
Done stringent limitation (<5μg/L).Some researchs are done for the treatment technology of stibium-containing wastewater both at home and abroad, at coagulating sedimentation, film
The treatment technologies such as reason technology, ion exchange.These technologies can remove antimony in water, but still it is efficient it is low, of high cost, complicated for operation,
The deficiencies of being also easy to produce secondary pollution, it is difficult to not only meet water standard but also take into account economy.
In recent years, domestic and foreign scholars propose safety economy, environmental-friendly, selectivity most has by force the microorganism of potential value
Absorption removes antimony technology.Screening, identification in relation to high resistance to antimony microorganism are the foundation stones that microorganism adsorption removes antimony technology, but this respect
Research is comparatively less.
Based on the above reasons, the application is proposed.
Invention content
The present invention is directed to problem and the shortcomings of the prior art pointed in background technology, the first object of the present invention
It is to provide a kind of high resistance to antimony Cattell proteus DSHN0704, second object of the present invention is to provide height described above resistance to
The separating screening method of antimony Cattell proteus DSHN0704, third object of the present invention are to provide above-mentioned height resistance to antimony Cattell
The application of proteus DSHN0704 can be used for the processing of soil containing antimony or waste water.
In order to realize that above-mentioned first purpose of the present invention, the technical solution adopted by the present invention be：A kind of high resistance to antimony Cattell change
Shape bacillus DSHN0704, Classification And Nomenclature are：Cattell proteus (Proteus cibarius) DSHN0704, in being preserved in
State's Type Tissue Collection (CCTCC), address：Wuhan, China Wuhan University, deposit number are：CCTCC NO：
M2018184, preservation date：On April 8th, 2018；The 16S rRNA sequences such as SEQ of high resistance to antimony Cattell proteus DSHN0704
Shown in ID No.1.
The resistance to antimony Cattell proteus DSHN0704 of height of the present invention, is to shave pollution from Lengshuijiang, Hunan tinnery
Separation screening obtains in soil, specifically screens and obtains through plate streaking.
The present invention resistance to antimony Cattell proteus (Proteus cibarius) DSHN0704 of height described above also has such as
Lower property：
1, microbial characteristic
The resistance to antimony Cattell proteus DSHN0704 of height of the present invention is Gram-negative bacteria, and the bacterium single bacterium colony is in milky white
Color, bacterium colony 1~3mm of size, protuberance, surface is smooth, and lawn is sticky, and thalline is that stub is rod-shaped, fission, micro- sem observation,
Bacterium colony is that form is as shown in Figure 1.
In order to realize that another object of the present invention, the present invention provide the resistance to antimony Cattell proteus of height described above
The separating screening method of DSHN0704, the method mainly include the following steps that：
It takes Lengshuijiang, Hunan tinnery to shave contaminated soil, is added in sterilizing bottle, is impregnated with sterile water, be 25 in temperature
10~30min is shaken on DEG C shaking table, stands 20~60s；Using serial dilutions separation of bacterial, after picking individual colonies, solid training
Purifying of crossing on base is supported, bacterium pure culture is carried out in 25 DEG C of constant incubators, obtains purifying bacterial strain, bacterial strain is through form, physiology
Biochemical character and 16S rDNA sequence analyses are accredited as Cattell proteus (Proteus cibarius), i.e. card of the invention
Family name's proteus DSHN0704 bacterial strains.
In order to realize that third object of the present invention, the present invention provide the resistance to antimony Cattell proteus of height described above
The application of DSHN0704 can be used for the improvement of soil containing antimony ion or waste water.
Applications of the Cattell proteus DSHN0704 as antimony ion adsorbent.
A kind of antimony ion adsorbent, the adsorbent include the Cattell proteus DSHN0704.
Compared with prior art, a kind of high resistance to antimony Cattell proteus DSHN0704 and its separation screening of the present invention
Methods and applications have following advantageous effect：
(1) the present invention provides the separation screenings of the bacterial strain to water body or Antimony In The Soils ion with extremely strong tolerance, excellent
Change culture, the phylogenetic tree of the Primary Construction bacterial strain of resistance to antimony and the strain storehouse of resistance to antimony；
(2) Cattell proteus bacterial strain DSHN0704 of the invention is Gram-negative bacteria, is had to high concentration antimony good
Tolerance performance, no matter either in solid medium, all there is preferable resistance to antimony performance, therefore the biocontrol bacteria exists in liquid
It can still survive in antimony content high solution and soil, can effectively realize that Sb (III) and Sb (V) are migrated in soil or water body
The control of conversion process reduces antimony toxicity and ecological hazard in soil or water body, final to realize effectively controlling for heavy metal Sb pollutions
Reason.
(3) Cattell proteus bacterial strain DSHN0704 bacterial strains of the invention have higher suction to antimony in water body (Sb) ion
Attached capacity, adsorption rate is fast, and the Spawn incubation condition of the present invention is simple, easy to maintain, is easy to industrialized production, to environment friend
It is good, there are good development and application potentiality, to microorganism adsorption except the development of antimony technology is of great significance.
Description of the drawings
Fig. 1 is the microscope photo of the resistance to antimony Cattell proteus DSHN0704 of height of the embodiment of the present invention 1；
Fig. 2 is the flat-plate bacterial colony form photo of the resistance to antimony Cattell proteus DSHN0704 of height of the embodiment of the present invention 1；
Fig. 3 is the transmission electron microscope photo of the resistance to antimony Cattell proteus DSHN0704 of height of the embodiment of the present invention 1；
Fig. 4 is the phylogenetic tree of the resistance to antimony Cattell proteus DSHN0704 of height of the embodiment of the present invention 1.
Specific implementation mode
It elaborates below to the case study on implementation of the present invention.The implementation case under the premise of technical solution of the present invention into
Row is implemented, and gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following realities
Apply case.
The information for including according to the application, to those skilled in the art can be easily to the essence of the present invention
Really description carries out various changes, without departing from spirit and scope of the appended claims.It should be understood that the scope of the present invention is not
Process, property or component defined by being confined to, because these embodiments and other descriptions are just for the sake of schematic
Illustrate certain aspects of the present disclosure.In fact, this field or those skilled in the relevant art obviously can be to embodiment party of the present invention
The various changes that formula is made all cover within the scope of the appended claims.
The separation screening of the high resistance to antimony Cattell proteus DSHN0704 of embodiment 1
The Cattell proteus of the present invention, generic name Proteus plant entitled cibarius, the entitled DSHN0704 of strain, bacterium
Strain is that separation screening obtains from ditch mud by the purification tank for liquid waste Rolling Stone area of Lengshuijiang, Hunan, is drawn especially by tablet
Line screening obtains, and the screening technique belongs to this field routine Isolation and screening of bacterial strain experimental method.Specific method and process description
It is as follows：The fresh mud of 10g is weighed, is added in the conical flask of sterilizing of 250ml；It takes 90ml sterile waters to impregnate, is 25 DEG C in temperature
20min is shaken on shaking table, stands 30s；Using serial dilutions separation of bacterial, after picking individual colonies, cross on solid medium
Purifying carries out bacterium pure culture in 25 DEG C of constant incubators, obtains purifying bacterial strain.The microscope photo of the bacterial strain is referring to Fig. 1.Bacterium
Strain flat-plate bacterial colony form photo is shown in Fig. 2, in addition, the strain is carried out physiological and biochemical property and 16S rDNA sequence analyses, and joins
According to document (Claesson MJ, O ' Sullivan O, Wang Q, et al.Comparative Analysis of
Pyrosequencing and a Phylogenetic Microarray for Exploring Microbial
Community Structures in the Human Distal Intestine.Ahmed N,ed.PLoS ONE.2009；4
(8):e6669；Parks DH,Tyson GW,Hugenholtz P,Beiko RG.STAMP:statistical analysis
of taxonomic and functional profiles.Bioinformatics.2014；30(21):3123-3124.),
It is accredited as Cattell proteus (Proteus cibarius), i.e. Cattell proteus DSHN0704 bacterial strains of the invention.
Photo shown in Fig. 1 is Cattell proteus DSHN0704 under laser confocal microscope, amplification 40 × 10 times
Microphoto；Photo shown in Fig. 2 is Cattell proteus DSHN0704 colonial morphology photos；Photo shown in Fig. 3 deforms for Cattell
The transmission electron microscope photo of bacillus DSHN0704.
The isolation and purification culture condition of the strain, detection viability condition are：Beef-protein medium, 10g albumen
Peptone powder, 5g sodium chloride, 5g beef extracts, 20g agar powders, 1000ml water, pH7.0 are cultivated at 25 DEG C.
The detection viability condition of the strain is：Beef-protein medium, 10g peptone powder, 5g sodium chloride, 5g oxen
Meat extract, 20g agar powders (solid medium is free of), 1000ml water, pH7.0, Sb (V) are cultivated at 25 DEG C.
The Molecular Identification of 2 Cattell proteus DSHN0704 of embodiment
The present embodiment carries out test analysis by the Cattell proteus DSHN0704 of the 16S rRNA sequence pair present invention.
16S rRNA correlation identification methods：
PCR identifications are carried out to the bacterial strain screened using the universal primer of bacterium：It is said by the operation of DNA of bacteria extracts kit
Bright book extracts template DNA.Use sense primer 5 '-GAGTTTGATCCTGGCTCAG-3 ' (such as SEQ of 16S rRNA conserved sequences
Shown in ID No.2) and downstream primer 5 '-TACGGTTACCTTGTTACGACTT-3 ' (as shown in SEQ ID No.3), to Cattell
The 16S rRNA genetic fragments of proteus DSHN0704 carry out PCR amplification, clone and are sequenced.
The segment that 1427bp is obtained after sequencing is MH613348 in the accession number of GenBank, the Pseudomonas is obtained by sequencing
See shown in SEQ ID No.1 in the sequencing result of Cattell proteus, amplified fragments.
16S rRNA gene sequencing BLAST results such as the following table 1 of present invention Cattell proteus DSHN0704 described above
It is shown.
BLAST result tables are sequenced in the 16S rRNA of 1 Cattell proteus DSHN0704 of table
The resistance to antimony Cattell proteus DSHN0704 bacterial strains of height that the present embodiment is above-mentioned to be separated based on 16S rRNA
Gene order comparison result is with the Neighbor- that V.furnisii ATCC 35016T (X74704.1) are outer branch structure
Joining phylogenetic trees are as shown in Figure 4.
Said gene test show Cattell proteus DSHN0704 (Proteus cibarius DSHN0704) with
There is Proteus cibarius JS9FJ796245 maximum similarity, the two similarity to reach 99.2992%.
Resistance to antimony performance test (solid medium, the Liquid Culture of the high resistance to antimony Cattell proteus DSHN0704 of embodiment 3
Base).
Solid medium：It is crossed on the beef extract-peptone solid medium of different antimony concentration using plate streaking,
Constant temperature incubation in 25 DEG C of constant incubators measures resistance to antimony performance.3 repetitions are arranged in every plant of bacterial strain for carrying out resistance to antimony performance measurement
Group.Finally obtain one plant of resistance to higher bacterial strain of antimony performance.High resistance to antimony Cattell proteus DSHN0704 containing antimony a concentration of 1~
Equal energy normal growth on the solid medium of 1000mg/L, a concentration of 1200mg/L growths of antimony are extremely slow, hardly grow.
Fluid nutrient medium：The bacterial strain of higher antimony concentration of solids culture based assays will be passed through, in the beef extract of higher concentration
The resistance to antimony performance detection of liquid is carried out in fluid nutrient medium.Cattell proteus DSHN0704 with higher resistance to antimony performance is in containing antimony
Beef extract-peptone fluid nutrient medium in cultivate 72 hours at 25 DEG C, take the 0.1mL bacterium solutions in being coated on the beef without antimony
It is cultivated in cream peptone solid culture, detects its performance of surviving.It is trained in the beef extract-peptone liquid of a concentration of 400mg/L containing antimony
It supports in base, Cattell proteus DSHN0704 still can preferably be grown.
Compared with other research reports, the resistance of Cattell proteus DSHN0704 counterweight metallic antimonies is reported higher than other
Microorganism.For bacterial strain long term survival in heavy metal pollution area, the strong resistance of counterweight metallic antimony may be with the existence ring of its complexity
Border is related.
The physio-biochemical characteristics test of the high resistance to antimony Cattell proteus DSHN0704 of embodiment 4
The enzyme activity of Cattell proteus DSHN0704, carbon assimilation test specific detection method：
First, prepare strip：Prepare one culture box, pour into about 5ml distilled water what disks honeycomb it is small it is recessed in, cause wet
Room；In disk along number bacterial strain number, takes out test bar and be put into disk, be placed in culture box.Then, inoculum prepares：Open a peace
Small jar NaCl0.85% (2ml), it is uniform in 1~4 point of pure single bacterium colony to suspension substratess of transfer needle picking, carefully grinding well, it is required
A concentration of 0.5 maxwell unit standard turbidity.Then, it is inoculated with：With same root suction pipe be inoculated with respectively brine bacteria suspension from NO3 to
PNPG developmental tubes；It opens an ampoule A PIAUX culture mediums and 200 μ l (6~8 drop), remaining physiological saline bacteria suspension to peace is added
Small jar, careful mixing avoid generating bubble；It fills by GLU to PAC culture mediums, pipe surface is smooth or slightly convex, and GLU, ADH and URE are then
It is covered with mineral oil, until the crescent of protrusion is formed；Culture box is covered, for 24 hours (± 2h) in 29 DEG C of ± 2 DEG C of cultures.
NO3 is tested：A drop NIT1 and one is added to drip in NIT2 reagents to NO3 pipes, it is red after five minutes to indicate positive reaction；Cause
The generation of nitrogen may draw and set negative reaction：Add in 2~3mgZn reagents to NO3 pipes, after five minutes, keeps colourless and show the positive
Reaction shows that there are still be reduced by Zn to nitrite, reaction result is the moon nitrate if color change is pink
Property.For identifying that the reaction of bacterium is nitrate reduction.When above-mentioned two, any one is the positive, and nitrate reduction reaction is sun
Property.
TRP is tested：Add a drop James experiments, reacts immediately, full pipe pink shows positive findings.
Assimilation experiments：Bacterial growth is observed, opaque cup portion shows positive reaction.
According to above-mentioned detection method, the enzyme activity of Cattell proteus DSHN0704 of the invention, the detection knot of carbon assimilation
Fruit difference is as shown in table 2.
The enzyme activity of 2 Cattell proteus DSHN0704 of table, carbon assimilation testing result table
+：Positive reaction；-：Negative reaction；Weakly positive is reacted
The utilization carbon source production acid of Cattell proteus DSHN0704 tests specific detection method：
First, strip prepares：Every strip is made of 5 small strips respectively, has 10 tubules in each small strip；Prepare
It is incubated box；Strain number is recorded in cartridge bottom；Add 10ml distilled water in being incubated in cassette bottom disk, it is made to fill up all holes, with
Keep the gaseous environment of moistening.2 small strip (0~19,20~39) is taken out, four small strip (0~9,10~19,20 is divided into
~29,30~39) it is put in, and all and is incubated in cassette bottom disk, take out another small strip (40~49) and be put in incubation cassette bottom disk
Behind above-mentioned 4 strips.Then, an ampoule NaCl0.85% (2ml) is opened, with transfer needle picking single bacterium colony to 10ml liquid
Body culture medium is carefully ground well uniform.Then, it is managed by 50 that bacteria suspension is added in strip claimed below with sterile sample injector
In：Cassette bottom disk will be incubated gently to tilt forward, bacteria suspension is added at the edge of cup in sample injector head rest, only fills it up with the part of pipe,
The top of pipe not add bacteria suspension, can keep managing interior anaerobic environment in this way.After the completion of inoculation, liquid level is answered smooth.Strip is put
It is incubated at 35 degrees Celsius.During strip is incubated, acid is produced in the tubule in strip under anaerobic environment, pH changes and makes training
Indicator discoloration in base is supported, thus observes fermentation.
Detection：Oxidation-bacterium makes pH indicator discolorations in strip hole by aerobic production acid.
Assimilation-can be shown when bacterium is sole carbon source using the different material in substrate in the hole in strip
Apparent bacterial growth.
According to above-mentioned detection method, the testing result that acid is produced using carbon source of Cattell proteus DSHN0704 of the invention
It is as shown in table 3 respectively.
The sour table of utilization carbon source production of 3 Cattell proteus DSHN0704 of table
+：Positive reaction；-：Negative reaction；
Embodiment 5：Cattell proteus DSHN0704 absorption properties are tested
Primary Study has been carried out to the antimony adsorption capacity of Cattell proteus DSHN0704 with shake flat experiment.9 250mL's
100mL beef-protein mediums are filled in conical flask, number is NO.1~9, is divided into 3 groups：(1) 1~No. 3 is A groups；(2)4
~No. 6 are B groups；(3) 7~No. 9 are C groups.A groups are not inoculated with, B, C group access 3% bacterium solution, 9 bottles be put into 30 DEG C,
It is cultivated in the shaking table of 180rpm.After for 24 hours, 3 bottles of C groups are carried out high-temperature sterilization (121 DEG C, 20min).Then in 9 bottles
Sb (III) is added in son, makes the final concentration of 20mg/L of Sb (III), then 9 bottles are put into shaking table and are cultivated.It, will after for 24 hours
Culture solution 8000rpm centrifugations 10min takes every group of mean values with the antimony concentration in aas determination supernatant
As experimental data, and calculate their antimony adsorption rate.
As known from Table 4, in the adsorption experiment of antimony, the concentration of antimony etc. is less than blank group in viable bacteria group B groups and inactivation group C groups
A groups.From the point of view of saving time and the energy, the suitable time of biological adsorption is for 24 hours.After Sb (III) effects for 24 hours, viable bacteria
Decline 5.64mg/L and 7.97mg/L respectively with the antimony ion concentration in inactivated bacteria solution, to Sb (III) removal rate in solution point
28.20% and 39.857% are not reached.The result shows that the viable bacteria of Cattell proteus DSHN0704 and inactivated bacteria are all to water environment
In antimony there is certain removal to act on, inactivated bacteria except antimony effect it is better than viable bacteria.Show the heavy metals removal and bacterial strain sheet of microorganism
The characteristics of body, is related, which has stronger heavy metal resistance, and growth is smaller by the toxic effect of heavy metal, it is in water process
There are biological adsorption, biological accumulation and exclusive effect in the process, and dead thalline has more adsorption site, does not have exclusive work
With, inactivated bacteria except antimony effect is better than viable bacteria.
Absorption properties of the 4 Cattell proteus DSHN0704 of table to Sb (III)
Sequence table
<110>University Of Science and Technology Of Hunan
<120>A kind of high resistance to antimony Cattell proteus DSHN0704 and its separating screening method and application
<130>Nothing
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1427
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 1
cagtctacac atgcagtcga gcggtaacag gagaaagctt gctttcttgc tgacgagcgg 60
cggacgggtg agtaatgtat ggggatctgc ccgatagagg gggataacta ctggaaacgg 120
tggctaatac cgcatgacgt ctacggacca aagcaggggc tcttcggacc ttgcgctatc 180
ggatgaaccc atatgggatt agctagtagg tgaggtaatg gctcacctag gcgacgatct 240
ctagctggtc tgagaggatg atcagccaca ctgggactga gacacggccc agactcctac 300
gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt 360
gtatgaagaa ggccttaggg ttgtaaagta ctttcagcgg ggaggaaggt gttaagatta 420
atactcttag caattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc 480
cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg 540
cggtcaatta agtcagatgt gaaagccccg agcttaactt gggaattgca tctgaaactg 600
gttggctaga gtcttgtaga ggggggtaga attccacgtg tagcggtgaa atgcgtagag 660
atgtggagga ataccggtgg cgaaggcggc cccctggaca aagactgacg ctcaggtgcg 720
aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgctgtaa acgatgtcga 780
tttagaggtt gtggtcttga accgtggctt ctggagctaa cgcgttaaat cgaccgcctg 840
gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg 900
agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tactcttgac atccagagaa 960
tcctttagag atagaggagt gccttcggga actctgagac aggtgctgca tggctgtcgt 1020
cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct tatcctttgt 1080
tgccagcacg tcatggtggg aactcaaagg agactgccgg tgataaaccg gaggaaggtg 1140
gggatgacgt caagtcatca tggcccttac gagtagggct acacacgtgc tacaatggca 1200
gatacaaaga gaagcgacct cgcgagagca agcggaactc ataaagtctg tcgtagtccg 1260
gattggagtc tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt agatcagaat 1320
gctacggtga atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt 1380
tgcaaaagaa gtaggtagct taaccttcgg gagggcgcta ccagctt 1427
<210> 2
<211> 19
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 2
gagtttgatc ctggctcag 19
<210> 3
<211> 22
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 3
tacggttacc ttgttacgac tt 22
Claims (5)
1. a kind of high resistance to antimony Cattell proteus DSHN0704, it is characterised in that：Its Classification And Nomenclature is：Cattell proteus
(Proteus cibarius) DSHN0704, has been preserved in China typical culture collection center (CCTCC), address：It is Chinese military
Chinese Wuhan University, deposit number are：CCTCC NO：M2018184, preservation date：On April 8th, 2018；High resistance to antimony Cattell becomes
The 16S rRNA sequences of shape bacillus DSHN0704 are as shown in SEQ ID No.1.
2. the separating screening method of the resistance to antimony Cattell proteus DSHN0704 of height described in claim 1, it is characterised in that：It is described
Method mainly includes the following steps that：
Lengshuijiang, Hunan tinnery antimony pollution soil is taken, is added in sterilizing bottle, is impregnated with sterile water, being 25 DEG C in temperature shakes
10~30min is shaken on bed, stands 20~60s；Using serial dilutions separation of bacterial, after picking individual colonies, solid medium
Upper scribing line purifying, carries out bacterium pure culture in 25 DEG C of constant incubators, obtains purifying bacterial strain, bacterial strain is through form, Physiology and biochemistry
Feature and 16S rDNA sequence analyses are accredited as Cattell proteus (Proteus cibarius), i.e. Cattell of the invention becomes
Shape bacillus DSHN0704 bacterial strains.
3. the application of the resistance to antimony Cattell proteus DSHN0704 of height described in claim 1, it is characterised in that：Can be used for containing antimony from
The improvement of sub- soil or waste water.
4. applications of the resistance to antimony Cattell proteus DSHN0704 of height described in claim 1 as antimony ion adsorbent.
5. a kind of antimony ion adsorbent, it is characterised in that：The adsorbent includes the resistance to antimony Cattell proteus of height
DSHN0704。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108865908A (en) * | 2018-07-16 | 2018-11-23 | 湖南科技大学 | A kind of high Rhodotorula mucilaginose of resistance to antimony DJHN070401 and its separating screening method and application |
| CN111676165A (en) * | 2020-06-24 | 2020-09-18 | 湖南科技大学 | Antimony-arsenic-oxidizing pseudomonas and application thereof |
| CN114540233A (en) * | 2022-02-25 | 2022-05-27 | 广东省科学院生态环境与土壤研究所 | Tailing soil sulfur oxidizing bacteria and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006069035A2 (en) * | 2004-12-21 | 2006-06-29 | Novozymes Biologicals, Inc. | Wastewater treatment compositions |
| CN105647842A (en) * | 2008-12-23 | 2016-06-08 | Reg生命科学有限责任公司 | Methods and compositions related to thioesterase enzymes |
| US20160369261A1 (en) * | 2013-06-14 | 2016-12-22 | Microvi Biotech Inc. | Novel biocatalyst compositions and processes for use |
| CN108865908A (en) * | 2018-07-16 | 2018-11-23 | 湖南科技大学 | A kind of high Rhodotorula mucilaginose of resistance to antimony DJHN070401 and its separating screening method and application |
| CN110699293A (en) * | 2019-11-06 | 2020-01-17 | 广东省生物资源应用研究所 | Method for screening bacteria with phosphate solubilizing function |
-
2018
- 2018-07-16 CN CN201810777168.1A patent/CN108676763B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006069035A2 (en) * | 2004-12-21 | 2006-06-29 | Novozymes Biologicals, Inc. | Wastewater treatment compositions |
| CN105647842A (en) * | 2008-12-23 | 2016-06-08 | Reg生命科学有限责任公司 | Methods and compositions related to thioesterase enzymes |
| US20160369261A1 (en) * | 2013-06-14 | 2016-12-22 | Microvi Biotech Inc. | Novel biocatalyst compositions and processes for use |
| CN108865908A (en) * | 2018-07-16 | 2018-11-23 | 湖南科技大学 | A kind of high Rhodotorula mucilaginose of resistance to antimony DJHN070401 and its separating screening method and application |
| CN110699293A (en) * | 2019-11-06 | 2020-01-17 | 广东省生物资源应用研究所 | Method for screening bacteria with phosphate solubilizing function |
Non-Patent Citations (4)
| Title |
|---|
| HANG DAI: "Proteus columbae sp. nov., isolated from a pigeon in Ma’anshan,China", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》 * |
| 肖桃: "变形杆菌比较基因组学分析及耐药基因岛的研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
| 邓仁健等: "响应曲面法优化Fe(Ⅲ)改性卡氏变形杆菌吸附去除Sb(Ⅴ)及其机理", 《环境科学研究》 * |
| 邵让: "耐锑卡氏变形杆菌筛选及其改性除锑研究", 《中国优秀硕士学位论文全文数据库基础科学辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108865908A (en) * | 2018-07-16 | 2018-11-23 | 湖南科技大学 | A kind of high Rhodotorula mucilaginose of resistance to antimony DJHN070401 and its separating screening method and application |
| CN108865908B (en) * | 2018-07-16 | 2021-07-06 | 湖南科技大学 | High-antimony-resistance rhodotorula mucilaginosa DJHN070401, and separation and screening method and application thereof |
| CN111676165A (en) * | 2020-06-24 | 2020-09-18 | 湖南科技大学 | Antimony-arsenic-oxidizing pseudomonas and application thereof |
| CN111676165B (en) * | 2020-06-24 | 2022-04-15 | 湖南科技大学 | Antimony-arsenic-oxidizing pseudomonas and application thereof |
| CN114540233A (en) * | 2022-02-25 | 2022-05-27 | 广东省科学院生态环境与土壤研究所 | Tailing soil sulfur oxidizing bacteria and application thereof |
| CN114540233B (en) * | 2022-02-25 | 2023-06-23 | 广东省科学院生态环境与土壤研究所 | Tailing soil sulfur oxidizing bacteria and application thereof |
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