CN107032505A - Composite bacteria agent for repairing lead waste water - Google Patents
Composite bacteria agent for repairing lead waste water Download PDFInfo
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- CN107032505A CN107032505A CN201710371052.3A CN201710371052A CN107032505A CN 107032505 A CN107032505 A CN 107032505A CN 201710371052 A CN201710371052 A CN 201710371052A CN 107032505 A CN107032505 A CN 107032505A
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- composite bacteria
- bacillus
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention belongs to microbial technology field, disclose the composite bacteria agent for repairing lead waste water, it is prepared by composite bacteria liquid and absorption carrier, characterized in that, the composite bacteria liquid includes Rhodopseudomonas palustris, bacillus stearothermophilus, into brilliant arthrobacterium, Acinetobacter calcoaceticus, xylose oxidation and bacillus zymotic fluid and Bacillus cercus.Composite bacteria agent of the present invention being capable of effectively remediating lead-contaminated waste water, and sludge yield is few.
Description
Technical field
The invention belongs to microbial technology field, and in particular to the composite bacteria agent for repairing lead waste water.
Background technology
In recent years, heavy metal pollution is on the rise, and seriously threatens the ecological balance, food security and health.Wherein
Lead（Pb）, cadmium（Cd）, mercury（Hg）, arsenic（As）, chromium（Cr）It is considered as " five poisonous creatures: scorpion, viper, centipede, house lizard, toad " in Main Heavy Metal.Lead is nature
Boundary's distribution very wide element, is also one of element for often using in industry, there is purposes widely in industrial and agricultural production.
Such as manufacture lead accumulator, pigment and paint, glass and other optical instruments and radiological defense clothes.Lead and its change
Compound is a kind of nondegradable environmental contaminants, can largely flow into environment by waste water,waste gas and industrial residue, passes through food chain, soil
Earth, water and air directly or indirectly enter human body.Internal blood lead concentration can be caused to raise, cause anaemia, cognitive impairment, hearing
The problems such as decrease, vitamin D metabolism disorder and abdominal pain.Research shows that damage of the lead to body is in multisystem, multiple organ
Property, to the system such as nerve, hematopoiesis, digestion, uropoiesis, reproduction, angiocarpy, endocrine, immune and it can grow and cause unfavorable shadow
Ring.And Lead Toxicity is persistently, long half time was difficult to be discharged by human body up to 10 years, and the lead contamination of any degree all can be strong to human body
Health has a negative impact.
The method of processing lead waste water mainly has physisorphtion, chemical precipitation method, bioanalysis etc. at present.Wherein physics is inhaled
Attached method is a kind of method of relatively effective processing heavy metal pollution, and the method has a good treatment effect, but its economical operation into
This height, it is difficult to large-scale application.Chemical precipitation method equipment is simple and convenient to operate, high treating effect, at present, to high concentration, big stream
The processing application of the lead waste water of amount is more universal, but to the consumption of chemical reagent it is big, costly, easily cause secondary pollution.It is raw
Thing rule includes phytoremediation and microorganism remediation, and although wherein phytoremediation treats both principal and secondary aspect of disease, but repairing efficiency is long, and investment is big.
Although microorganism remediation processing cost is low, it is small that surrounding enviroment are disturbed, do not produce secondary pollution, suitable microorganism resource is few,
Repairing effect is not obvious.
The content of the invention
In order to overcome the deficiencies in the prior art, the invention provides the composite bacteria agent for repairing lead waste water.The present invention
Composite bacteria agent being capable of effectively remediating lead-contaminated waste water, and sludge yield is few.
The present invention adopts the following technical scheme that realization：
Composite bacteria agent for repairing lead waste water, is prepared by composite bacteria liquid and absorption carrier,
The composite bacteria liquid includes Rhodopseudomonas palustris, bacillus stearothermophilus, into brilliant arthrobacterium, calcium acetate not lever
Bacterium, xylose oxidation and bacillus and Bacillus cercus.
Further, the composite bacteria liquid is prepared according to following technique：
By Rhodopseudomonas palustris, bacillus stearothermophilus, into brilliant arthrobacterium, Acinetobacter calcoaceticus, xylose oxidation and bacillus,
Bacillus cercus obtains zymotic fluid through inclined-plane culture, shake-flask seed culture and fermentation tank culture respectively；By the red false unit cell in marsh
Fermented liquid, bacillus stearothermophilus zymotic fluid, into brilliant pole fermented liquid, Acinetobacter calcoaceticus zymotic fluid, xylose oxygen
Change bacillus fermentation liquid and bacillus cereus fermentation liquid according to 2-3:2-3:3-5:3-5:4-7:4-7 volume ratio mixing is equal
It is even, obtain composite bacteria liquid.
Further,
The absorption carrier is prepared according to following technique：Polystyrene microsphere is added to ultrasonic disperse 8min in ethanol,
Then add under ammoniacal liquor and 3- mercaptopropyl trimethoxysilanes, argon gas protective condition, be warming up to 50 DEG C, 200rpm stirring 3h stop
Only stir, naturally cool to room temperature, precipitation is collected by centrifugation, be freeze-dried, produce absorption carrier；The polystyrene microsphere, second
The mass ratio of alcohol, ammoniacal liquor and 3- mercaptopropyl trimethoxysilanes is 5:10:2:1.
Further,
The composite bacteria agent is prepared according to following technique：In the composite bacteria liquid that absorption carrier is put into 2-3 times of weight,
100rpm stir culture 3h, stop stirring, then carry out low temperature drying, and drying temperature is 15 DEG C, and water content is after drying
8wt%, packaging, is produced.
Preferably, the Rhodopseudomonas palustris zymotic fluid, bacillus stearothermophilus zymotic fluid, into brilliant arthrobacterium fermentation
Liquid, Acinetobacter calcoaceticus zymotic fluid, the concentration of xylose oxidation and bacillus zymotic fluid and bacillus cereus fermentation liquid are controlled
1 × 1010cfu/ml。
Preferably, the Rhodopseudomonas palustris is ATCC 17001, and the bacillus stearothermophilus is ATCC
12976, described is ATCC 15481 into brilliant arthrobacterium, and the Acinetobacter calcoaceticus is ATCC 33304, the xylose oxidation
Bacillus is ATCC 15173, and said waxy bacillus is ATCC 10876；
Further,
According to the present invention, there is no particular limitation for the form of the microbial inoculum added into the environment containing lead contamination, as long as protecting
The microbial inoculum can work in the environment containing lead contamination after card is added, the form of the microbial inoculum of addition,
It can be changed as needed.
The present invention also has no particular limits to the quantity of bacterial strain in the zymotic fluid of addition, and this can contain lead according to described
Content in the environment of pollution determines, for example, when the higher existence for the microbial inoculum of the lead content in the environment less
When sharp, the inoculum concentration of the microbial inoculum can be improved；When the lead content in the environment is relatively low or existence to bacterial strain of the environment
Influence it is smaller when, it is possible to reduce inoculum concentration.
Strain of the present invention belongs to known bacterial strain, can be commercially available from commercial sources such as ATCC.The present invention's
Inclined-plane culture, shake-flask seed culture and the fermentation tank culture of each strain are the cellar culture mode of this area, are not wounds of the present invention
New point, is not described in detail herein.
The beneficial effect that the present invention is obtained mainly includes but is not limited to the following aspects：
Composite bacteria agent of the present invention is prepared using absorption carrier and mixed bacteria liquid, and specific surface area is big, and thalline adhesive force is strong, density
It is suitable with water body, can suspend with water body, it is to avoid preparation density is excessive, and to be deposited in the microbial profile that bottom of pond causes uneven
And influence scavenging effect, moreover it is possible to the yield of sludge is reduced, is conducive to the removal of pollutant in waste liquid；
Absorption carrier of the present invention is prepared simply, with low cost, and with functional functional group, high adsorption capacity；
Reasonable compatibility between six kinds of bacterial strains, each bacterial strain has been used in the composite bacteria agent of the present invention, symbiosis is coordinated, mutually not antagonism, shape
Into the strain of dominant microflora, high efficiency composition microbial inoculum is configured to, is adapted to the lead contamination water body of processing high concentration, additionally it is possible to remove COD
Pollutant, has purified water body.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, have below in conjunction with the application
Body embodiment, the technical scheme to the application is clearly and completely described, it is clear that described embodiment is only this Shen
Please a part of embodiment, rather than whole embodiment.Based on the embodiment in the application, those of ordinary skill in the art are not having
There is the every other embodiment made and obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
Composite bacteria agent for repairing lead waste water, it is prepared according to following technique：
By Rhodopseudomonas palustris ATCC 17001, bacillus stearothermophilus ATCC 12976, into brilliant arthrobacterium ATCC
15481st, Acinetobacter calcoaceticus ATCC 33304, xylose oxidation and bacillus ATCC 15173, Bacillus cercus ATCC 10876
Respectively zymotic fluid is obtained through inclined-plane culture, shake-flask seed culture and fermentation tank culture；The concentration of above-mentioned six kinds of zymotic fluids is controlled
1 × 1010cfu/ml；
By Rhodopseudomonas palustris zymotic fluid, bacillus stearothermophilus zymotic fluid, motionless into brilliant pole fermented liquid, calcium acetate
Bacillus fermentation liquid, xylose oxidation and bacillus zymotic fluid and bacillus cereus fermentation liquid are according to 2:2:3:3:4:4 volume ratio is mixed
Close uniform, obtain composite bacteria liquid；
Polystyrene microsphere is added to ultrasonic disperse 8min in ethanol, ammoniacal liquor and 3- mercapto propyl trimethoxy silicon is then added
Under alkane, argon gas protective condition, 50 DEG C are warming up to, 200rpm stirring 3h stop stirring, naturally cool to room temperature, it is heavy to be collected by centrifugation
Form sediment, freeze-drying produces absorption carrier；The polystyrene microsphere, ethanol, ammoniacal liquor and 3- mercaptopropyl trimethoxysilanes
Mass ratio is 5:10:2:1；
In the composite bacteria liquid that absorption carrier is put into 2 times of weight, 100rpm stir culture 3h stop stirring, then carried out low
Temperature is dried, and drying temperature is 15 DEG C, and water content is 8wt% after drying, and packaging is produced.
Embodiment 2
Composite bacteria agent for repairing lead waste water, it is prepared according to following technique：
By Rhodopseudomonas palustris ATCC 17001, bacillus stearothermophilus ATCC 12976, into brilliant arthrobacterium ATCC
15481st, Acinetobacter calcoaceticus ATCC 33304, xylose oxidation and bacillus ATCC 15173, Bacillus cercus ATCC 10876
Respectively zymotic fluid is obtained through inclined-plane culture, shake-flask seed culture and fermentation tank culture；The concentration of above-mentioned six kinds of zymotic fluids is controlled
1 × 1010cfu/ml；
By Rhodopseudomonas palustris zymotic fluid, bacillus stearothermophilus zymotic fluid, motionless into brilliant pole fermented liquid, calcium acetate
Bacillus fermentation liquid, xylose oxidation and bacillus zymotic fluid and bacillus cereus fermentation liquid are according to 3:3:5:5:7:7 volume ratio is mixed
Close uniform, obtain composite bacteria liquid；
Polystyrene microsphere is added to ultrasonic disperse 8min in ethanol, ammoniacal liquor and 3- mercapto propyl trimethoxy silicon is then added
Under alkane, argon gas protective condition, 50 DEG C are warming up to, 200rpm stirring 3h stop stirring, naturally cool to room temperature, it is heavy to be collected by centrifugation
Form sediment, freeze-drying produces absorption carrier；The polystyrene microsphere, ethanol, ammoniacal liquor and 3- mercaptopropyl trimethoxysilanes
Mass ratio is 5:10:2:1；
In the composite bacteria liquid that absorption carrier is put into 3 times of weight, 100rpm stir culture 3h stop stirring, then carried out low
Temperature is dried, and drying temperature is 15 DEG C, and water content is 8wt% after drying, and packaging is produced.
Embodiment 3
The lead of lead waste water removes experiment：
Sample；Take lead contamination water sample, wherein lead concentration 179mg/L, COD 634mg/L；
Handling process：The pH of lead waste water is adjusted to 7, is then uniformly thrown by every cubic metre of liquid and spreads 20 grams of composite bacteria agent, it is raw
Thing is handled one week, by liquid by plate filter filtration sterilization, is finally discharged, and Pb is determined with ICP-AES methods2+Content.
Group：With the composite bacteria agent example of embodiment 1, pollutant data are measured by sampling；And control group is set, microbial inoculum is detected
In each bacterial strain effect；
Control group 1：Without Rhodopseudomonas palustris, remaining be the same as Example 1；
Control group 2：Without bacillus stearothermophilus, remaining be the same as Example 1；
Control group 3：Without into brilliant arthrobacterium, remaining be the same as Example 1；
Control group 4：Without Acinetobacter calcoaceticus, remaining be the same as Example 1；
Control group 5：Without xylose oxidation and bacillus, remaining be the same as Example 1；
Control group 6：Without Bacillus cercus, remaining be the same as Example 1；
The specific testing result of each pollutant is shown in Table 1 after processing：
Table 1
| Group | Lead mg/L | COD mg/L |
| Embodiment 1 | 5.7 | 9.6 |
| Control group 1 | 20.3 | 62.8 |
| Control group 2 | 15.9 | 31.4 |
| Control group 3 | 28.5 | 43.0 |
| Control group 4 | 14.6 | 50.7 |
| Control group 5 | 31.4 | 28.2 |
| Control group 6 | 17.5 | 68.3 |
Conclusion：Mushroom reasonable compatibility in the composite bacteria agent of the present invention, concertedness is strong, coordinates absorption carrier, can effectively remove
The pollutants such as lead, COD.
Embodiment 4
The density measurement of composite bacteria agent of the present invention：
Test group：Composite bacteria agent prepared by the embodiment of the present invention 1；
Control group：Selection diatomite is carrier, remaining be the same as Example 1..
The indexs such as the density and sludge yield of composite bacteria agent are have detected, 2 are specifically shown in Table：
Table 2
| Index | Density g/ml | 10d sludge yields g/L | 20d sludge yields g/L |
| Test group | 1.06 | 4.3 | 11.4 |
| Control group | 1.37 | 9.8 | 25.7 |
Conclusion：Composite bacteria agent density and waste water density of the present invention are quite, non-breakable, can be suspended in waste water, it is to avoid heavy
Product wadding collection produces substantial amounts of sludge to bottom of pond, and is also equipped with preferable adsorption effect.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, the present invention not
It is limited to above example, there can also be many deformations.One of ordinary skill in the art can be direct from present disclosure
All deformations for exporting or associating, are considered as protection scope of the present invention.
Claims (6)
1. the composite bacteria agent for repairing lead waste water, is prepared, it is characterised in that described by composite bacteria liquid and absorption carrier
Composite bacteria liquid includes Rhodopseudomonas palustris, bacillus stearothermophilus, into brilliant arthrobacterium, Acinetobacter calcoaceticus, xylose oxygen
Change bacillus and Bacillus cercus.
2. composite bacteria agent according to claim 1, it is characterised in that the composite bacteria liquid prepared according to following technique and
：
By Rhodopseudomonas palustris, bacillus stearothermophilus, into brilliant arthrobacterium, Acinetobacter calcoaceticus, xylose oxidation and bacillus,
Bacillus cercus obtains zymotic fluid through inclined-plane culture, shake-flask seed culture and fermentation tank culture respectively；By the red false unit cell in marsh
Fermented liquid, bacillus stearothermophilus zymotic fluid, into brilliant pole fermented liquid, Acinetobacter calcoaceticus zymotic fluid, xylose oxygen
Change bacillus fermentation liquid and bacillus cereus fermentation liquid according to 2-3: 2-3:3-5:3-5:4-7:4-7 volume ratio mixing is equal
It is even, obtain composite bacteria liquid.
3. composite bacteria agent according to claim 1 or 2, it is characterised in that the absorption carrier is prepared according to following technique
And obtain：
Polystyrene microsphere is added to ultrasonic disperse 8min in ethanol, ammoniacal liquor and 3- mercapto propyl trimethoxy silicon is then added
Under alkane, argon gas protective condition, 50 DEG C are warming up to, 200rpm stirring 3h stop stirring, naturally cool to room temperature, it is heavy to be collected by centrifugation
Form sediment, freeze-drying produces absorption carrier；The polystyrene microsphere, ethanol, ammoniacal liquor and 3- mercaptopropyl trimethoxysilanes
Mass ratio is 5:10:2:1.
4. composite bacteria agent according to claim 3, it is characterised in that the composite bacteria agent prepared according to following technique and
：In the composite bacteria liquid that absorption carrier is put into 2-3 times of weight, 100rpm stir culture 3h stop stirring, then carried out low
Temperature is dried, and drying temperature is 15 DEG C, and water content is 8wt% after drying, and packaging is produced.
5. the composite bacteria agent according to claim 2-4 is allowed to one, it is characterised in that the Rhodopseudomonas palustris fermentation
Liquid, bacillus stearothermophilus zymotic fluid, into brilliant pole fermented liquid, Acinetobacter calcoaceticus zymotic fluid, xylose oxidation and bacillus
The concentration of zymotic fluid and bacillus cereus fermentation liquid is controlled 1 × 1010cfu/ml。
6. the composite bacteria agent according to right wants 5, it is characterised in that the Rhodopseudomonas palustris is ATCC 17001, institute
Bacillus stearothermophilus is stated for ATCC 12976, it is described into brilliant arthrobacterium be ATCC 15481, the Acinetobacter calcoaceticus
For ATCC 33304, the xylose oxidation and bacillus is ATCC 15173, and said waxy bacillus is ATCC 10876.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107083345A (en) * | 2017-06-05 | 2017-08-22 | 蒋瑞忠 | For the preparation technology for the microorganism formulation for handling leaded sewage |
| CN107699498A (en) * | 2017-10-04 | 2018-02-16 | 李萍 | A kind of complex microorganism preparations of degrading organic phosphor pesticides and preparation method thereof |
| CN107983304A (en) * | 2017-11-28 | 2018-05-04 | 重庆科技学院 | Efficient high-temperature resistant type acticarbon |
| CN111235077A (en) * | 2017-10-27 | 2020-06-05 | 裘正荣 | Composite biological agent for repairing polychlorinated biphenyl polluted soil |
| CN116693067A (en) * | 2023-07-14 | 2023-09-05 | 中国水产科学研究院珠江水产研究所 | In-situ restoration method for jointly treating cultivation tail water by utilizing duckweed-loaded plant growth promoting bacteria |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107083345A (en) * | 2017-06-05 | 2017-08-22 | 蒋瑞忠 | For the preparation technology for the microorganism formulation for handling leaded sewage |
| CN107699498A (en) * | 2017-10-04 | 2018-02-16 | 李萍 | A kind of complex microorganism preparations of degrading organic phosphor pesticides and preparation method thereof |
| CN111235077A (en) * | 2017-10-27 | 2020-06-05 | 裘正荣 | Composite biological agent for repairing polychlorinated biphenyl polluted soil |
| CN107983304A (en) * | 2017-11-28 | 2018-05-04 | 重庆科技学院 | Efficient high-temperature resistant type acticarbon |
| CN116693067A (en) * | 2023-07-14 | 2023-09-05 | 中国水产科学研究院珠江水产研究所 | In-situ restoration method for jointly treating cultivation tail water by utilizing duckweed-loaded plant growth promoting bacteria |
| CN116693067B (en) * | 2023-07-14 | 2024-02-06 | 中国水产科学研究院珠江水产研究所 | In-situ restoration method for jointly treating cultivation tail water by utilizing duckweed-loaded plant growth promoting bacteria |
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Application publication date: 20170811 |