CN102719381A - Composite microbial agent and preparation method and application thereof - Google Patents
Composite microbial agent and preparation method and application thereof Download PDFInfo
- Publication number
- CN102719381A CN102719381A CN201210204602XA CN201210204602A CN102719381A CN 102719381 A CN102719381 A CN 102719381A CN 201210204602X A CN201210204602X A CN 201210204602XA CN 201210204602 A CN201210204602 A CN 201210204602A CN 102719381 A CN102719381 A CN 102719381A
- Authority
- CN
- China
- Prior art keywords
- fermented liquid
- rhodopseudomonas palustris
- rhodococcus erythropolis
- microbial inoculum
- acinetobacter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of environmental microbiology, and discloses a composite microbial agent and a preparation method and application in the municipal biological sewage treatment thereof. The composite microbial agent consists of rhodococcus erythropolis fermentation broth, acinetobacter sp. fermentation broth, rhodopseudomonas palustris fermentation broth, and bacillus subtilis fermentation broth, wherein the bactericidal concentration of each of the fermentation broth reaches above 109 cfu/ml. The composite microbial agent provided by the invention achieves the synergistic degrading effect that each strain cannot achieve separately by the synergistic effect of each microbial strain of different proportions, and has guiding significance and reference value for the municipal sewage treatment.
Description
Technical field
The invention belongs to the environmental microorganism technical field, relate in particular to a kind of composite fungus agent and preparation method and its application in biological municipal sewage is handled.
Background technology
Along with the quickening of urban development process, constantly perfecting of the raising of people's environmental consciousness and laws and regulations makes to municipal wastewater treatment requirement and emission standard increasingly stringent.Add composite fungus agent, to the processing and up to standard the having important practical significance of municipal wastewater.
Municipal wastewater is mainly handled through biological process at present, and wherein the microorganism species in the mud plays a major role.But municipal wastewater factory is through the industrial sewage of regular meeting's reception dealing with complicated, and the poisonous hard-degraded substance that increases in the industrial sewage increases, and has increased the difficulty that municipal wastewater is handled to a certain extent.Simultaneously, since the impact and the variation of water quality, the also corresponding increase of the difficulty up to standard of each item effluent index.The all contaminations qualitative change of therefore, how to remove efficiently in the sewage gets important further.
Therefore; Utilize a kind of complex microbial inoculum, each pollutant of specificity ground degraded not only can improve the whole competitiveness and the stability of microorganism species; And can increase substantially the degradation efficiency of each pollutant, the biological treatment of municipal wastewater is had actual using value.
Summary of the invention
The purpose of this invention is to provide a kind of complex microbial inoculum, apply it in the municipal wastewater processing through different ratio combinations, thereby improve the municipal wastewater overall treatment efficiency, promote that water outlet is up to standard comprehensively.
In order to solve the problems of the technologies described above, the present invention is able to solve through following technical proposals:
A kind of complex microbial inoculum; Be made up of rhodococcus erythropolis (Rhodococcus erythropolis) fermented liquid, acinetobacter calcoaceticus (Acinetobacter sp.) fermented liquid, Rhodopseudomonas palustris (Rhodopseudomonas palustris) fermented liquid, subtilis (Bacillus subtilis) fermented liquid, bacterium is dense in above-mentioned four kinds of fermented liquids reaches 10 respectively
9More than the cfu/ml.
As preferably, the unit dry cell weight of described rhodococcus erythropolis (Rhodococcus erythropolis) fermented liquid, acinetobacter calcoaceticus (Acinetobacter sp.) fermented liquid, Rhodopseudomonas palustris (Rhodopseudomonas palustris) fermented liquid and subtilis (Bacillus subtilis) fermented liquid is than being (1-6): (1-4): (1-5): (2-8).
As preferably, the unit dry cell weight of described rhodococcus erythropolis (Rhodococcus erythropolis) fermented liquid, acinetobacter calcoaceticus (Acinetobacter sp.) fermented liquid, Rhodopseudomonas palustris (Rhodopseudomonas palustris) fermented liquid and subtilis (Bacillus subtilis) fermented liquid is than being (2-4): (1-2): (2-3): (4-6).
The preparation method of this complex microbial inoculum is following:
With rhodococcus erythropolis (Rhodococcus erythropolis), acinetobacter calcoaceticus (Acinetobacter sp.), Rhodopseudomonas palustris (Rhodopseudomonas palustris) and subtilis (Bacillus subtilis) respectively from-80 ℃ of glycerine pipes the picking culture in the activation of ruling of general activating solid substratum; Single colony inoculation after the activation in the corresponding liquid substratum, is reached minimum 10 through enlarged culturing respectively until bacterium is dense then
9Cfu/ml;
The enlarged culturing liquid of above-mentioned four kinds of bacterial classifications is mixed, make the per dry wt content of four kinds of bacterial classifications in the mixed solution than being (1-6): (1-4): (1-5): (2-8), obtain the liquid composite fungus agent thus; Add absorption carrier-wheat bran in the liquid composite fungus agent, can get the solid composite fungus agent.
As preferably, the per dry wt content of described rhodococcus erythropolis (Rhodococcus erythropolis), acinetobacter calcoaceticus (Acinetobacter sp.), Rhodopseudomonas palustris (Rhodopseudomonas palustris) and subtilis (Bacillus subtilis) is than being (2-4): (1-2): (2-3): (4-6).
This complex microbial inoculum is used for municipal wastewater etc., but fast denitrogenation dephosphorization, degraded COD realizes the gain effect that municipal wastewater is handled.
Above-mentioned four kinds of bacterial classifications are respectively rhodococcus erythropolis (Rhodococcus erythropolis) Sunda 1101; Acinetobacter calcoaceticus (Acinetobacter sp.) Sunda 1001; Rhodopseudomonas palustris (Rhodopseudomonas palustris) 1.2349; Subtilis (Bacillus subtilis) 1.1473.
Rhodococcus erythropolis (Rhodococcus erythropolis) Sunda 1101 is a strain aerobic denitrifying bacteria, and culture presevation is numbered CCTCC M 2011211.Acinetobacter calcoaceticus (Acinetobacter sp.) Sunda 1001 is the aerobic dephosphorization bacterial of a strain, and culture presevation is numbered CCTCC M 2011210.More than two strain bacterial classifications all derive from Chinese typical culture collection center.
Rhodopseudomonas palustris (Rhodopseudomonas palustris) 1.2349, subtilis (Bacillus subtilis) 1.1473 all derive from Chinese common micro-organisms culture presevation administrative center.
The present invention has significant technique effect owing to adopted above technical scheme:
Complex microbial inoculum provided by the invention, the different ratios through each microbial strains in the composite fungus agent acts synergistically, and has reached each bacterial classification Synergistic degradation effect that is beyond one's reach separately, municipal wastewater is handled meaning and the reference function that directiveness is arranged.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail:
The preparation of embodiment 1 complex microbial inoculum
General activation medium (g/L): tryptone 10, yeast extract 5, NaCl 10, pH7.0.
The enlarged culturing base (g/L) of acinetobacter calcoaceticus: Carnis Bovis seu Bubali cream 3, peptone 10, NaCl 5, KH
2PO
40.02, pH7.2-7.4.
The enlarged culturing base (g/L) of rhodococcus erythropolis: KNO
31; KH
2PO
41; FeSO
47H
2O 0.05; CaCl
20.02; MgSO
47H
2O 1; Soduxin 6H
2O 8.5; PH7.0.
The enlarged culturing base (g/L) of subtilis: glucose 5; Peptone 5; Yeast powder 1; Carnis Bovis seu Bubali cream 3; MgSO
47H
2O 0.5; MnSO
4H
2O 0.005; PH7.0.
The cultivation enlarged culturing base (g/L) of Rhodopseudomonas palustris: Sodium Propionate 2.7, sodium acetate 0.45, yeast extract paste 0.5, NH
4H
2PO
41.2, MgSO
47H
2O 0.2, pH 6.5-7.2.
Rhodococcus erythropolis (Rhodococcus erythropolis) Sunda 1101, acinetobacter calcoaceticus (Acinetobacter sp.) Sunda 1001, Rhodopseudomonas palustris (Rhodopseudomonas palustris) 1.2349 and subtilis (Bacillus subtilis) 1.1473 respectively from-80 degree glycerine pipes the picking culture in the activation of ruling of general activating solid substratum; Single colony inoculation after the activation in 5ml corresponding liquid substratum, is reached minimum 10 through enlarged culturing respectively until bacterium is dense then
9Cfu/ml.
The enlarged culturing liquid of above-mentioned rhodococcus erythropolis (Rhodococcus erythropolis) Sunda 1101, acinetobacter calcoaceticus (Acinetobacter sp.) Sunda 1001, Rhodopseudomonas palustris (Rhodopseudomonas palustris) 1.2349 and subtilis (Bacillus subtilis) 1.1473 is mixed according to the volume ratio of a bit; Make the per dry wt content of four kinds of bacterial classifications in the mixed solution than being (2-4): (1-2): (2-3): (4-6); Obtain the liquid composite fungus agent thus; If in liquid bacterial agent, add absorption carrier-wheat bran, then can be made into the solid composite fungus agent.
Embodiment 2: the lab scale experiment of complex microbial inoculum in simulated wastewater
Preparation simulation municipal wastewater (mg/L): Soduxin 5010; (NH4)
2SO
4330; Peptone 100; NaCl 80; CaCO
323; KH
2PO
423; MgSO
494; NaHCO
365; Ferrous sulfate 0.2; PH 7.5～8.0.
Preparation 10L simulated wastewater adds a certain amount of complex microbial inoculum in the plastics bucket, continue aeration, and timing sampling detects the variation of ammonia nitrogen, total nitrogen, total phosphorus and COD.Experimental result shows behind the 6h that ammonia nitrogen, total nitrogen, total phosphorus and COD have reached 95%, 89%, 91%, 93% respectively in the simulated wastewater.
Embodiment 3: the lab scale experiment of complex microbial inoculum in actual municipal wastewater
Measure actual municipal wastewater water sample of 8L and 2L aeration tank mud mixture respectively; It is mixed place the plastics bucket, establish two groups of processing, one group is blank; Promptly be made up of sewage and active sludge, another group is for adding a certain amount of complex microbial inoculum on the basis of blank.Two groups of processing are continued aeration, and timing sampling detects the variation of ammonia nitrogen, total nitrogen, total phosphorus and COD.Experimental result is compared with blank after showing 6h, and the processing of having added complex microbial inoculum has increased by 46%, 30%, 30% and 40% respectively than blank on the clearance of ammonia nitrogen, total nitrogen, total phosphorus and COD.Explain and added complex microbial inoculum, good gain effect has been played in the processing of actual municipal wastewater.
Embodiment 4: the application of complex microbial inoculum in municipal wastewater is handled
The complex microbial inoculum that preparation is accomplished adds to by 0.05% addition and carries out synergy in certain municipal wastewater treatment plant aeration tank and handle.Compare with control group, complex microbial inoculum can improve about 20% with the overall treatment efficiency of municipal wastewater.Therefore, complex microbial inoculum has the important use meaning in the synergy of municipal wastewater is handled.
In a word, the above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to the covering scope of patent of the present invention.
Claims (8)
1. complex microbial inoculum; It is characterized in that: this composite fungus agent is made up of rhodococcus erythropolis (Rhodococcus erythropolis) fermented liquid, acinetobacter calcoaceticus (Acinetobacter sp.) fermented liquid, Rhodopseudomonas palustris (Rhodopseudomonas palustris) fermented liquid, subtilis (Bacillus subtilis) fermented liquid, and bacterium is dense in above-mentioned four kinds of fermented liquids reaches 10 respectively
9More than the cfu/ml.
2. complex microbial inoculum according to claim 1 is characterized in that: the unit dry cell weight of said rhodococcus erythropolis (Rhodococcus erythropolis) fermented liquid, acinetobacter calcoaceticus (Acinetobacter sp.) fermented liquid, Rhodopseudomonas palustris (Rhodopseudomonas palustris) fermented liquid and subtilis (Bacillus subtilis) fermented liquid is than being (1-6): (1-4): (1-5): (2-8).
3. complex microbial inoculum according to claim 2 is characterized in that: the unit dry cell weight of said rhodococcus erythropolis (Rhodococcus erythropolis) fermented liquid, acinetobacter calcoaceticus (Acinetobacter sp.) fermented liquid, Rhodopseudomonas palustris (Rhodopseudomonas palustris) fermented liquid and subtilis (Bacillus subtilis) fermented liquid is than being (2-4): (1-2): (2-3): (4-6).
4. according to the preparation method of the arbitrary described complex microbial inoculum of claim 1-3, it is characterized in that method is following:
With rhodococcus erythropolis (Rhodococcus erythropolis), acinetobacter calcoaceticus (Acinetobacter sp.), Rhodopseudomonas palustris (Rhodopseudomonas palustris) and subtilis (Bacillus subtilis) respectively from-80 ℃ of glycerine pipes the picking culture in the activation of ruling of general activating solid substratum; Single colony inoculation after the activation in the corresponding liquid substratum, is reached minimum 10 through enlarged culturing respectively until bacterium is dense then
9Cfu/ml;
The enlarged culturing liquid of above-mentioned four kinds of bacterial classifications is mixed, make the per dry wt content of four kinds of bacterial classifications in the mixed solution than being (1-6): (1-4): (1-5): (2-8), obtain the liquid composite fungus agent thus.
5. the preparation method of complex microbial inoculum according to claim 4 is characterized in that: add absorption carrier-wheat bran in the described liquid composite fungus agent, get the solid composite fungus agent.
6. the preparation method of complex microbial inoculum according to claim 4 is characterized in that: the per dry wt content of described rhodococcus erythropolis (Rhodococcus erythropolis), acinetobacter calcoaceticus (Acinetobacter sp.), Rhodopseudomonas palustris (Rhodopseudomonas palustris) and subtilis (Bacillus subtilis) is than being (2-4): (1-2): (2-3): (4-6).
7. the arbitrary described complex microbial inoculum of claim 1-3 is used for the synergy processing of municipal wastewater.
8. the application of complex microbial inoculum according to claim 7 is characterized in that: complex microbial inoculum is added to by 0.05% addition carry out synergy in the municipal wastewater and handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210204602XA CN102719381A (en) | 2012-06-20 | 2012-06-20 | Composite microbial agent and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210204602XA CN102719381A (en) | 2012-06-20 | 2012-06-20 | Composite microbial agent and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102719381A true CN102719381A (en) | 2012-10-10 |
Family
ID=46945254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210204602XA Pending CN102719381A (en) | 2012-06-20 | 2012-06-20 | Composite microbial agent and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102719381A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560858A (en) * | 2015-01-26 | 2015-04-29 | 鹭滨环保科技（上海）有限公司 | Rhodococcus erythropolis, construction method thereof and application in sewage treatment |
| CN107032505A (en) * | 2017-05-24 | 2017-08-11 | 蒋瑞忠 | Composite bacteria agent for repairing lead waste water |
| CN107227282A (en) * | 2017-08-08 | 2017-10-03 | 苏州克莱尔环保科技有限公司 | A kind of extraordinary flora of sewage disposal and its cultural method |
| CN108423838A (en) * | 2018-02-08 | 2018-08-21 | 普乐格（厦门）生物科技有限公司 | One kind is for water-based microorganism formulation of ecological safety and preparation method thereof |
| CN108793603A (en) * | 2018-06-25 | 2018-11-13 | 成都市市政工程（集团）有限责任公司 | Municipal domestic sewage processing method |
| CN110240285A (en) * | 2019-06-19 | 2019-09-17 | 广东睿洁环保工程有限公司 | A kind of composite bacteria agent reducing COD value in serious pollution waste water |
| CN110938557A (en) * | 2019-11-01 | 2020-03-31 | 湖南景翌湘台环保高新技术开发有限公司 | Composite microbial inoculum for degrading COD (chemical oxygen demand) in wastewater and preparation method thereof |
| CN110980962A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Phosphorus-accumulating microbial inoculum and sludge treatment technology using same |
| CN111423993A (en) * | 2020-03-18 | 2020-07-17 | 自然资源部天津海水淡化与综合利用研究所 | Algae-based EM microbial inoculum and preparation method thereof |
| CN111847658A (en) * | 2020-06-16 | 2020-10-30 | 广东省石油与精细化工研究院 | Composite microbial inoculum for enhancing nitrogen and phosphorus removal of sewage as well as preparation method and application thereof |
| CN114437956A (en) * | 2020-11-05 | 2022-05-06 | 中石化石油工程技术服务有限公司 | Microbial composition for efficiently degrading human excrement and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698539A (en) * | 2009-10-13 | 2010-04-28 | 王颖 | Microecological agent for purifying water and preparation method thereof |
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN102146347A (en) * | 2009-12-18 | 2011-08-10 | 浙江天科高新技术发展有限公司 | Acinetobacter sp. and application of composite bacterial agent of acinetobacter species |
-
2012
- 2012-06-20 CN CN201210204602XA patent/CN102719381A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698539A (en) * | 2009-10-13 | 2010-04-28 | 王颖 | Microecological agent for purifying water and preparation method thereof |
| CN102146347A (en) * | 2009-12-18 | 2011-08-10 | 浙江天科高新技术发展有限公司 | Acinetobacter sp. and application of composite bacterial agent of acinetobacter species |
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560858A (en) * | 2015-01-26 | 2015-04-29 | 鹭滨环保科技（上海）有限公司 | Rhodococcus erythropolis, construction method thereof and application in sewage treatment |
| CN107032505A (en) * | 2017-05-24 | 2017-08-11 | 蒋瑞忠 | Composite bacteria agent for repairing lead waste water |
| CN107227282A (en) * | 2017-08-08 | 2017-10-03 | 苏州克莱尔环保科技有限公司 | A kind of extraordinary flora of sewage disposal and its cultural method |
| CN108423838B (en) * | 2018-02-08 | 2020-09-11 | 普乐格（厦门）生物科技有限公司 | Microbial preparation for ecological safety water system and preparation method thereof |
| CN108423838A (en) * | 2018-02-08 | 2018-08-21 | 普乐格（厦门）生物科技有限公司 | One kind is for water-based microorganism formulation of ecological safety and preparation method thereof |
| CN108793603A (en) * | 2018-06-25 | 2018-11-13 | 成都市市政工程（集团）有限责任公司 | Municipal domestic sewage processing method |
| CN110240285A (en) * | 2019-06-19 | 2019-09-17 | 广东睿洁环保工程有限公司 | A kind of composite bacteria agent reducing COD value in serious pollution waste water |
| CN110938557A (en) * | 2019-11-01 | 2020-03-31 | 湖南景翌湘台环保高新技术开发有限公司 | Composite microbial inoculum for degrading COD (chemical oxygen demand) in wastewater and preparation method thereof |
| CN110980962A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Phosphorus-accumulating microbial inoculum and sludge treatment technology using same |
| CN111423993A (en) * | 2020-03-18 | 2020-07-17 | 自然资源部天津海水淡化与综合利用研究所 | Algae-based EM microbial inoculum and preparation method thereof |
| CN111847658A (en) * | 2020-06-16 | 2020-10-30 | 广东省石油与精细化工研究院 | Composite microbial inoculum for enhancing nitrogen and phosphorus removal of sewage as well as preparation method and application thereof |
| CN114437956A (en) * | 2020-11-05 | 2022-05-06 | 中石化石油工程技术服务有限公司 | Microbial composition for efficiently degrading human excrement and application thereof |
| CN114437956B (en) * | 2020-11-05 | 2024-05-17 | 中石化石油工程技术服务有限公司 | Microbial composition for efficiently degrading human body excrement and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102719381A (en) | Composite microbial agent and preparation method and application thereof | |
| CN103374524B (en) | Salt-tolerant microbial agent and preparation method thereof | |
| Mohana et al. | Biodegradation and decolourization of anaerobically treated distillery spent wash by a novel bacterial consortium | |
| CN106635861B (en) | A kind of de- COD denitrification microorganism microbial inoculum of salt tolerant and preparation method thereof | |
| CN103373762B (en) | Biological denitrification method for salt-containing sewage | |
| CN108165509B (en) | Compound micro-ecological preparation and preparation method thereof for the black smelly water harnessing in river | |
| CN105586294B (en) | One plant of acinetobacter calcoaceticus and its application in phosphorus is denitrogenated in waste water | |
| CN103374525B (en) | Wastewater treating microbial agent and preparation method thereof | |
| CN103373758B (en) | Denitrifying method for ammonia-containing waste water discharged in acrylic production process | |
| CN110217895B (en) | Compound microbial agent for water environment treatment and application thereof | |
| CN107760625B (en) | Compound microbial agent for treating river water with different pollution degrees | |
| CN110240285A (en) | A kind of composite bacteria agent reducing COD value in serious pollution waste water | |
| CN104611279B (en) | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes | |
| CN102220240A (en) | PM-I sludge reduction microbial agent | |
| CN113957004B (en) | Flavobacterium aureum and application thereof in preparation of halophyte epiphyte repair maintenance microbial inoculum | |
| CN106115939A (en) | A kind of online bio-synergistic ammonia nitrogen removal method of sewage | |
| CN103305443B (en) | Preparation and method for restoring ammonia nitrogen-containing industrial sewage | |
| CN102965322B (en) | Manganese oxidizing composite microbial system and application thereof | |
| CN111440747B (en) | Compound microbial agent for sewage treatment, microecological preparation and application | |
| Cheng et al. | Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water | |
| CN104450563B (en) | The bacterial strains of one kind solution ornithine Raoul bacterium GJ 5 and its application | |
| CN106520611B (en) | Handle efficient composite bacteria agent and its application of organic wastewater with difficult degradation thereby with high salt | |
| CN103373766A (en) | Wastewater biochemical treatment method for simultaneously realizing sludge reduction | |
| CN106119150B (en) | A kind of dephosphorization microbial bacterial agent and its preparation method and application | |
| CN105502805B (en) | Enhancement microbiological multistep treatment sanitary sewage and the processing system and domestic sewage processing method of recycling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20121010 |