CN108423838A - One kind is for water-based microorganism formulation of ecological safety and preparation method thereof - Google Patents
One kind is for water-based microorganism formulation of ecological safety and preparation method thereof Download PDFInfo
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The present invention provides one kind for water-based microorganism formulation of ecological safety and preparation method thereof, is related to water-treatment technology field.It includes composite bacteria：The red spirillum of Rhodopseudomonas palustris 10~20%, pichia farinose 20~30%, bacillus amyloliquefaciens 20~30%, rhodococcus erythropolis 0~15%, lactobacillus plantarum 20~30%, outer sulphur 5~15%；And the carrier for loading composite bacteria.Preparation method is：Composite bacteria is added in culture solution, fermented and cultured obtains zymocyte liquid；Zymocyte liquid is concentrated, addition porous-starch, after stirring and adsorbing, adds dextrin and elbaite micro mist, activated carbon fibre is disposed therein 2~3h of absorption, it is dry.Various strains play mutually synergistic effect in the microorganism formulation, effectively repair water body, form beneficial micro-ecological environment, and strain is fixed on carrier, effectively improve purification, the repairing effect of microorganism.
Description
Technical field
The present invention relates to water-treatment technology fields, and it is more particularly to a kind of for the water-based microorganism formulation of ecological safety and
Preparation method.
Background technology
The water environment pollution getting worse in China, untreated breeding wastewater and industry, the discharge of sanitary sewage make water
Body is contaminated, and eutrophication is serious, blue-green alge is broken out, putrefactive microorganisms mass propagation, the life of breeding water body or landscape water body
State environment is destroyed, and heavy losses are caused to culture fishery or natural environment, therefore, how to be repaired to water body, structure
Ecological safety water system is built to be of great significance.
In the prior art, water remediation mainly has physical method, chemical method and biological method.Physical method and chemistry side
There is the problems such as quantities is big, of high cost, improvement is not thorough in method.And the biological method based on microorganism remediation has uniqueness
Effect, by microorganism in water body pollutant and eutrophy ingredient absorbed, degraded and converted, can be fundamentally
Realize purification of water quality.But in the prior art, it is carrying out often carrying out water body processing only with more single strain, to water
The repairing effect of body is poor.Such as patent CN02156977.0 discloses a kind of low temperature nitrification microbial inoculum and application thereof, is related to hamburger
Bacterium nitrobacter, Nitrobacter winogradskyi, Bacillus cercus, bacillus licheniformis and saccharomyces cerevisiae totally six kinds of bacterial strains, these bacterial strains
After culture, becomes low temperature resistant nitrification microbial inoculum by its culture equal volume proportions group, there is removal at a lower temperature
The effect of super low concentration ammonia nitrogen is used alone or is adapted to corresponding bioreactor, can be used for sea-farming and batch production follows
The removal processing of the ammonia nitrogen removal and COD of ring seawater high-density breeding.Nitrifier is only applicable to cold temperature culture environment in this technology
In sea-farming, application range is narrow, meanwhile, it needs to coordinate with corresponding bioreactor, composite bacteria is more, step is numerous
It is trivial, it is unfavorable for promoting and applying in production.
Invention content
The purpose of the present invention is to provide one kind being used for the water-based microorganism formulation of ecological safety, this microorganism formulation can
Effectively repair contaminated water body.
Another object of the present invention is to provide a kind of preparation method for the water-based microorganism formulation of ecological safety, works
Skill is simple, easy to operate.
The present invention solves its technical problem using following technical scheme to realize.
The present invention proposes that one kind being used for the water-based microorganism formulation of ecological safety, including：
Composite bacteria, in percentage by weight, including Rhodopseudomonas palustris 10~20%, pichia farinose 20~
30%, the red spirillum of bacillus amyloliquefaciens 20~30%, rhodococcus erythropolis 0~15%, lactobacillus plantarum 20~30%, outer sulphur 5
~15%；And the carrier for loading the composite bacteria.
The present invention proposes a kind of preparation method for the water-based microorganism formulation of ecological safety, includes the following steps：
It is prepared by S1, composite bacteria：Proportionally strain is carried out to be mixed to get the composite bacteria, by the composite bacteria
It is added in culture solution, closed cultivate 5~10 days obtains zymocyte liquid under the conditions of 28~32 DEG C；
It is prepared by S2, carrier：The zymocyte liquid is concentrated to give concentration bacterium solution, is added porous-starch, after stirring and adsorbing, then
Dextrin and elbaite micro mist is added, obtains maceration extract, activated carbon fibre is arranged in 2~3h of absorption in the maceration extract, is done
It is dry.
The advantageous effect for water-based microorganism formulation of ecological safety and preparation method thereof of the embodiment of the present invention is：
The present invention utilizes the effect of composite bacteria mutually cooperateed with, and can efficiently use excessive C, N, P member in water environment
Element converts it into beneficial microbe itself, to reduce the BOD in water environment, COD level, forms beneficial Tiny ecosystem ring
Border, purifying water body, the use for reducing chemosterilant, promotes the growth of cultivated animals.The invention can be applied to aquaculture water
Body purifies, the reparation of the water-based structure of ecological safety and landscape water body.
Secondly, the present invention realizes consolidating for strain by the way that composite bacteria to be fixed on porous-starch, and by adding dextrin
Fixed and embedding effect, can effectively improve the treatment effect of composite bacteria, avoid because being directly seeded in caused bacterium in water body
The bad phenomenons such as body loss.Secondly, there is the porous-starch microballoon of strain to be adsorbed in active carbon fiber fabrics load, loaded
The microorganism formulation of type, use and convenient transportation.By it when in applied to water body, active carbon fiber fabrics are first to having in water body
Malicious harmful substance is adsorbed, and avoids composite bacteria in early period by excessive loss, and realizes double purified effect to water body.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
The preparation method for the water-based microorganism formulation of ecological safety of the embodiment of the present invention is carried out specifically below
It is bright.
It is provided in an embodiment of the present invention a kind of for the water-based microorganism formulation of ecological safety, including：
Composite bacteria, in percentage by weight, including Rhodopseudomonas palustris 10~20%, pichia farinose 20~
30%, the red spirillum of bacillus amyloliquefaciens 20~30%, rhodococcus erythropolis 0~15%, lactobacillus plantarum 20~30%, outer sulphur 5
~15%；And
Carrier, for loading the composite bacteria.
Further, in a preferred embodiment of the present invention, in percentage by weight, the composite bacteria includes that marsh is red
Pseudomonad 15~20%, pichia farinose 20~25%, bacillus amyloliquefaciens 25~30%, rhodococcus erythropolis 5~
10%, the red spirillum of lactobacillus plantarum 20~25%, outer sulphur 5~10%.
Rhodopseudomonas palustris belongs to one kind of photosynthetic bacteria, photosynthetic bacteria abbreviation PSB, is strain most ancient on the earth
One of, PSB thalline are full of nutrition, and protein content is up to 65%, and rich in bioactive substances such as multivitamin, coenzyme and
Trace element is adaptable, can restrain oneself the organic wastewater of high depth and stronger decomposition and inversion ability, have one to poisonous substances such as phenol, cyanogen
Surely it endures and the features such as capacity of decomposition.Pichia farinose (Pichia farinose) is high to the tolerance level of ammonia nitrogen, can go
Except ammonia nitrogen, there is good result especially for the ammonia emission in animal wastes.Bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) to person poultry harmless, antibiotic and antiseptic protein polypeptide isoreactivity substance can be generated, for dynamic
Plant growth has facilitation.The degradable hydrocarbons pollutant of rhodococcus erythropolis (Rhodococcus erythropolis) takes off
Sulphur etc., lactobacillus plantarum (Lactobacillus plantarum) can degrading nitrite etc., and promote growth of animal.Outside
The red spirillum of sulphur (Ectothiorhodospira shaposhnikovii) can be using inorganic matters such as hydrogen sulfide as electron donor also
Former carbon dioxide, synthesis of organic substance, the effectively sulfide in degradation sewage.By the compounding of above-mentioned several strains, can play
Good synergistic effect can not only effectively carry out water body purification, repair, additionally it is possible to promote the life of animal or plant in water body
It is long, enhance the immunity and disease resistance of aquatic livestock.
Further, in a preferred embodiment of the present invention, the Rhodopseudomonas palustris (Rhodopseudomonas
Palustris) it is strain that deposit number is ACCC10649, the pichia farinose (Pichia farinose) is preservation
The strain that number is ACCC20025, the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are compiled for preservation
Number strain for being ACCC06476, the rhodococcus erythropolis (Rhodococcus erythropolis) is for deposit number
The strain of ACCC02667, it is ACCC11118 that the lactobacillus plantarum (Lactobacillus plantarum), which is deposit number,
Strain, it is ACCC19809 that the outer red spirillum of sulphur (Ectothiorhodospira shaposhnikovii), which is deposit number,
Strain.
Further, above-mentioned each strain carries out the production of hybrid seeds in accordance with the following methods：
The Rhodopseudomonas palustris that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
In the conical flask of (Rhodopseudomonaspalustris ACCC10649) photosynthetic bacteria culture medium of transferring, in 28-32
DEG C, illumination cultivation 60-84 hours is taken out after culture medium muddiness.
Pichia farinose (the Pichiafarinose that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
ACCC20025 it) transfers in I culture medium of wort agar, 25-30 DEG C of cultivation temperature, 180-220rpm shaken cultivations 36-60 is small
When, it is taken out after culture medium muddiness.
Bacillus amyloliquefaciens (the Bacillus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Amyloliquefaciens ACCC06476) in nutrient agar culture medium of transferring, the 180-220rpm at 32-38 DEG C
Shaken cultivation 36-60 hours, takes out after culture medium muddiness.
Rhodococcus erythropolis (the Rhodococcus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Erythropolis ACCC02667) in LB culture mediums of transferring, cultivated 60-84 hours at 28-32 DEG C, wait for culture medium muddiness
After take out.
Lactobacillus plantarum (the Lactobacillus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Plantarum ACCC11118) MRS fluid nutrient mediums of transferring conical flask in, cultivated 48-72 hours at 35-39 DEG C, wait training
It is taken out after supporting base muddiness.
Red spirillum (the Ectothiorhodospira of outer sulphur that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Shaposhnikovii ACCC19809) photosynthetic bacteria culture medium of transferring conical flask in, at 28-32 DEG C, illumination cultivation 60-
It 84 hours, is taken out after culture medium muddiness.
It is taken out after the above Spawn incubation, is then placed in 2-4 DEG C of refrigerator and preserves for use.
After obtaining each strain, proportionally above-mentioned each strain is carried out being mixed to get the composite bacteria, by composite bacteria
It is added in culture solution, closed cultivate 5~10 days obtains zymocyte liquid under the conditions of 28~32 DEG C.Wherein, culture solution includes：Sugar
Honey and water.
Further, in a preferred embodiment of the present invention, the mass ratio of composite bacteria and culture solution is 1：80~120.
Further, in a preferred embodiment of the present invention, in parts by weight, culture solution includes 8~12 parts of molasses, water
90~96 parts.
Further, in a preferred embodiment of the present invention, in parts by weight, culture solution further includes brown sugar 10~15
Part.
In the prior art, when discharging microorganism, typically directly by microbial inoculant in pending water body, but by
In environmental factor, Competition etc., it is easy to microorganism be caused to be lost in.Especially in the more complicated water body of environment, strain holds
It is vulnerable to the damage of noxious material, the survival rate of microorganism is low, and reproduction speed is slow.Because the effect of microorganism remediation water body is past
It is past undesirable.Therefore, above method acquisition is met strain and is fixed on carrier by the present invention, specific as follows：
It is prepared by carrier：The zymocyte liquid that fermentation obtains is concentrated to give concentration bacterium solution, porous-starch, stirring and adsorbing is added
Afterwards, dextrin and elbaite micro mist are added, maceration extract is obtained.Then activated carbon fibre is arranged in the maceration extract and is adsorbed
2~3h is dried to obtain the microorganism formulation of support type.
Further, in a preferred embodiment of the present invention, zymocyte liquid volume is concentrated into be concentrated for 1/4~1/5
Bacterium solution, the mass ratio for concentrating bacterium solution and porous-starch is 1:0.5~0.7.
Further, the condition of stirring and adsorbing is：0.5~1.5h is adsorbed under the conditions of 80~120r/min.Porous-starch
It is to be had and be similar in hornet's nest in the hydrolysate obtained less than various starch of degrading under starch gelatinization temperature by hydrolase
Hollow structure, surface are distributed a large amount of aperture, and bulk density is low, adsorption capacity is strong, under agitation, you can fully adsorbs micro- life
Object.And after absorption composite bacteria to it with good protective effect, effective protection, reproduction speed can be formed to composite bacteria
Soon, water remediation effect is good.In the present embodiment, porous-starch can be commercial product, can also be according to prior art preparation (example
The method as disclosed in CN1546529A), it is no longer repeated herein.
Further, in a preferred embodiment of the present invention, it is 1 that dextrin, which includes mass ratio,:1~2 beta-cyclodextrin and malt
The mass ratio of dextrin, concentration bacterium solution, elbaite micro mist and dextrin is 1:0.1~0.2:0.05~0.08.
After obtained strain to be composite is adsorbed onto on porous-starch, beta-cyclodextrin and maltodextrin is added, encapsulating layer can be formed,
Further composite bacteria is protected, passes through preservation, the transport etc. for being conducive to microorganism formulation.And a certain amount of lithium electricity is added
Gas stone micro mist has electrical and reactivity forever, can generate negative oxygen ion, and anion forms subset with water molecules
Water promotes the oxygen content in water to increase, improves the survival rate of strain.Meanwhile anion is conducive to activating cell, and it is dynamic to improve aquatic products
The premunition etc. of object.
Further, in a preferred embodiment of the present invention, the mass ratio for concentrating bacterium solution and active carbon fiber fabrics is 1:0.3~
0.5。
Further, the specific surface area of active carbon fiber fabrics active carbon fiber fabrics is 1200~1800m2/ g, average pore size
It is 0.1~1 μm.There are a large amount of micropores on active carbon fiber fabrics, and there is high specific surface area, activated carbon fibre to be arranged in leaching
When in stain liquid, in dipping process, can absorption carriage have the porous-starch particle of composite bacteria, form the microorganism of support type
Preparation.When the microorganism formulation is applied in water body, on the one hand active carbon fiber fabrics can first purify water body, especially
The heavy metal ion such as effective Adsorption of Lead, chromium, effective protection composite bacteria can be adsorbed.On the other hand, on active carbon fiber fabrics
A large amount of micropores can ensure good water absorbency and water retentivity, effectively facilitate the breeding of microorganism.After microorganism mass propagation,
The micropore of active carbon fiber fabrics can also the fixed site of microbial body offer absorption.By dual fixation and dual suction-operated,
Reach good water body purification, repairing effect.Protect microbial cells not by extraneous adverse environment (such as acid, alkali, harmful ion
Deng) influence, nor affect on mass transfer, nutriment to and product export, keep strain high activity.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of microorganism formulation provided in this embodiment is made according to following steps：
(1) production of hybrid seeds is produced：
The Rhodopseudomonas palustris that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
In the conical flask of (Rhodopseudomonaspalustris ACCC10649) photosynthetic bacteria culture medium of transferring, at 30 DEG C, light
According to culture 72 hours, taken out after culture medium muddiness.
Pichia farinose (the Pichia farinose that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
ACCC20025 it) transfers in I culture medium of wort agar, 28 DEG C of cultivation temperature, 200rpm shaken cultivations 48 hours wait cultivating
It is taken out after base muddiness.
Bacillus amyloliquefaciens (the Bacillus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Amyloliquefaciens ACCC06476) in nutrient agar culture medium of transferring, the 210rpm shaken cultivations at 35 DEG C
It 48 hours, is taken out after culture medium muddiness.
Rhodococcus erythropolis (the Rhodococcus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Erythropolis ACCC02667) in LB culture mediums of transferring, cultivates 72 hours at 30 DEG C, taken after culture medium muddiness
Go out.
Lactobacillus plantarum (the Lactobacillus that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Plantarum ACCC11118) MRS fluid nutrient mediums of transferring conical flask in, cultivated 60 hours at 37 DEG C, wait for that culture medium is muddy
It is taken out after turbid.
Red spirillum (the Ectothiorhodospira of outer sulphur that will be bought from Chinese agriculture Microbiological Culture Collection administrative center
Shaposhnikovii ACCC19809) photosynthetic bacteria culture medium of transferring conical flask in, at 30 DEG C, illumination cultivation 72 is small
When, it is taken out after culture medium muddiness.
It is taken out after the above Spawn incubation, is then placed in 2-4 DEG C of refrigerator and preserves for use.
(2) prepared by composite bacteria：The strain obtained according to following weight percent comparison step (1) be mixed to get compound
Strain：It is Rhodopseudomonas palustris 18%, pichia farinose 24%, bacillus amyloliquefaciens 26%, lactobacillus plantarum 24%, outer
The red spirillum of sulphur 8%.By composite bacteria according to 1:100 ratio is added in culture solution, closed culture 7 days under the conditions of 30 DEG C,
Zymocyte liquid is obtained after pH is less than 4.20.Wherein, culture solution includes：10 parts by weight molasses, 15 parts of brown sugar and 90 weight
Part water.
(3) prepared by carrier：The zymocyte liquid that step (2) obtains is concentrated into volume and obtains concentration bacterium solution (100g) for 1/5,
65g porous-starch is added, after 100r/min stirring and adsorbings 1h.Add 2g beta-cyclodextrins, 4g maltodextrins and 15g elbaites
Micro mist obtains maceration extract.Then 40g activated carbon fibre is arranged in maceration extract and adsorbs 2.5h, spray drying obtains support type
Microorganism formulation.
Embodiment 2
A kind of microorganism formulation provided in this embodiment is made according to following steps：
(1) production of hybrid seeds is produced：It is prepared Following the procedure of Example 1.
(2) prepared by composite bacteria：The strain obtained according to following weight percent comparison step (1) be mixed to get compound
Strain：It is Rhodopseudomonas palustris 12%, pichia farinose 28%, bacillus amyloliquefaciens 22%, lactobacillus plantarum 28%, outer
The red spirillum of sulphur 10%.By composite bacteria according to 1:100 ratio is added in culture solution, closed culture 7 days under the conditions of 30 DEG C,
Zymocyte liquid is obtained after pH is less than 4.20.Wherein, culture solution includes：10 parts by weight molasses, 15 parts of brown sugar and 90 weight
Part water.
(3) prepared by carrier：The zymocyte liquid that step (2) obtains is concentrated into volume and obtains concentration bacterium solution (100g) for 1/4,
65g porous-starch is added, after 100r/min stirring and adsorbings 1h.Add 2g beta-cyclodextrins, 4g maltodextrins and 15g elbaites
Micro mist obtains maceration extract.Then 40g activated carbon fibre is arranged in maceration extract and adsorbs 2.5h, spray drying obtains support type
Microorganism formulation.
Comparative example 1
A kind of microorganism formulation that this comparative example provides, which is that the embodiment of the present invention 1 is obtained, concentrates bacterium solution.
Test example 1
In order to verify applicable cases of the invention formulation on culture of Penaeus vannamei, in July, 2016 in Spring in Pig House of Shanghai Fengxian
One farm in area is tested.
(1) test material and method：
1, pond：2 mouthfuls of the Selection experiment pool, area are respectively 17.8 mu and 18 mu, number D1, D2.Compare 2 mouthfuls of the pool, face
Product is respectively 18.4 mu and 19 mu, number D3, D4.
2, Penaeus Vannmei seedling：Shrimp seedling comes from Hainan Island and Xiamen of Fujian Province, is raised and train by locality desalination and reaches 1.5~2 lis
Meter Hou Yi is put into above-mentioned each pond, and shrimp seedling bouncing used finds no quality problems.
3, mixed feed：Overall process uses the high-quality mixed feed that Aquatic Product Technique Popularization Station, Shanghai City is recommended.
4, disinfecting drug：It is cleaned up the pond successively disinfection using tea seed cake, quick lime and bleaching powder before putting in a suitable place to breed in all ponds.Usually
Daily disinfection is carried out to pond using halogen family disinfectant according to even in everyday situations timing.
5, fertilizer：Each pond uses sufficient base manure, by constant application of organic fertilizers, cultivates basic food organisms.Usually according to water quality shape
Chemical fertilizer is applied in the appropriate top dressing of condition according to actual needs.
6, breeding water：Finite quantity water coke slurry mode is used in principle.Water is only added each pond early period not drain until highest water
Position, the experiment pool are not added with water in mid-term and do not change water, to keep water level, suitably supplement natural evaporation, stage changes water, Shao Liangtian on a small quantity
Add.The control pool suitably carried out changing water according to actual needs with external source water quality condition in the middle and later periods.
7, microorganism formulation：The microorganism formulation that control pool D3 is provided using comparative example 1, control pool D4 do not use microorganism
Preparation, experiment pool D1 then use the microorganism formulation that the embodiment of the present invention 1 provides, experiment pool D2 then to use the embodiment of the present invention 2
The microorganism formulation of offer.
Dosage usage is：
Microorganism formulation：The microorganism formulation that experiment pool D1 and D2 is used by the water surface per acre concentrates bacterium solution, shearing containing 100g
At the small pieces of 1cm or so, uniformly discharge.
It compares pool D3 and concentrates bacterium solution using 100g by the water surface per acre, uniformly splashed after diluting 50-100 times.
Every month adds a microbial bacterial agent, 3 months experimental periods (No. 7.1~No. 9.30).
8, other：Aerator is equipped with, using and other facility conditions and management method, test the pool and compare the basic phase in the pool
Together.
(2) result and analysis
1, it puts in a suitable place to breed and harvest situation：
17.8 mu of pool D1 areas are tested, put 750,000 tail of Penaeus Vannmei seedling in a suitable place to breed, mu puts 4.21 ten thousand tails, harvests total output 5696
Kilogram, average yield per mu 320kg, average mu is got a profit 2645 yuan.
18.1 mu of pool D1 areas are tested, put 760,000 tail of Penaeus Vannmei seedling in a suitable place to breed, mu puts 4.20 ten thousand tails, harvests total output 5629
Kilogram, average yield per mu 311kg, average mu is got a profit 2571 yuan.
18.4 mu of pool D3 areas are compareed, put 780,000 tail of Penaeus Vannmei seedling in a suitable place to breed, average mu puts 4.24 ten thousand tails, harvests total output
4471kg, average yield per mu 243kg, average mu are got a profit 1047 yuan.
19 mu of pool D4 areas are compareed, put 810,000 tail of Penaeus Vannmei seedling in a suitable place to breed, average mu puts 4.26 ten thousand tails, harvests total output
2685kg, average yield per mu 141kg all lose.
2, incidence：
Experiment pool D1, D2 overall process is not fallen ill.And pool D3 site morbidities are compareed, situation is lighter.It is more to compare pool D4 morbidities,
And control is hardly resulted in after the onset, crop is seriously affected, the later stage has to carry out collecting to it in advance.
3, water quality situation：
Testing the pool, water quality is more stable from beginning to end, and planktonic organism Compositional balance, every conventional index is chronically at normally
State, food platform checks totally, is not smeared with dirt.And the water quality for compareing the pool subregions D3 is more unstable, integral level is unbalanced.
More unstable in the water-quality ratio of control pool D4, bacterium phase, algae are mutually fluctuated acutely, and food platform inspection often speckles with many dirts, foul.
During cultivation, the detection of water quality index is carried out to four culture ponds belonging to centre for spreading, has referred to table 1：
Table 1
a：It is measured using plug disk method；
b：It is the oxidation-reduction potential at 2~3cm to measure depth；
c：The pond shifts to an earlier date collecting.
4, substrate situation：
It is less to test pool substrate cleaning, hardboard, mud, and compares that pool D3 substrate situations are uneven, and partial picture is good, portion
Point poor, control pool D4 substrate situations are very poor, and mud is thicker, gather a large amount of dirts, foul, the serious blacking of feed band, stench, product
Gather a large amount of dead shrimps.
5, it ingests situation：
Experiment pool shrimp ingests normally, and feed efficiency is high, and feed coefficient is low, digests and absorbs completely, excreta is bright
It is aobvious to reduce, and compare the pool parts D3 fishes and shrimps and ingest abnormal, control pool D4 often shows as shrimp and takes the photograph since environmental condition is relatively poor
Eat abnormal, efficiency of feed utilization is low, and feed coefficient increases.
6, growing state：
Pool shrimp body well-grown is tested, vigor is good, and harvest specification is big, and shrimp body health, organoleptic indicator is normal, through the related power in city
The product quality sampling inspection of prestige department is qualified, and the shrimp weight for compareing pool D3 is uneven, and control pool D4 growths are significantly affected,
It is relatively small to harvest specification, individual is irregular significantly, and quality declines.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. one kind being used for the water-based microorganism formulation of ecological safety, which is characterized in that including：
Composite bacteria, in percentage by weight, including Rhodopseudomonas palustris 10~20%, pichia farinose 20~
30%, the red spirillum of bacillus amyloliquefaciens 20~30%, rhodococcus erythropolis 0~15%, lactobacillus plantarum 20~30%, outer sulphur 5
~15%；And
Carrier, for loading the composite bacteria.
2. according to claim 1 be used for the water-based microorganism formulation of ecological safety, which is characterized in that according to weight percent
Than meter, the composite bacteria includes Rhodopseudomonas palustris 15~20%, pichia farinose 20~25%, solution starch gemma bar
The red spirillum of bacterium 25~30%, rhodococcus erythropolis 5~10%, lactobacillus plantarum 20~25%, outer sulphur 5~10%.
3. a kind of preparation method for the water-based microorganism formulation of ecological safety as described in claim 1~2 any one,
It is characterized by comprising the following steps：
It is prepared by S1, composite bacteria：Proportionally strain is carried out to be mixed to get the composite bacteria, the composite bacteria is added
Into culture solution, closed cultivate 5~10 days obtains zymocyte liquid under the conditions of 28~32 DEG C；
It is prepared by S2, carrier：The zymocyte liquid is concentrated to give concentration bacterium solution, is added porous-starch, after stirring and adsorbing, is added
Dextrin and elbaite micro mist, obtain maceration extract, and activated carbon fibre is arranged in 2~3h of absorption in the maceration extract, dry.
4. the preparation method according to claim 3 for the water-based microorganism formulation of ecological safety, which is characterized in that step
In rapid S1, the mass ratio of the composite bacteria and the culture solution is 1：80~120.
5. the preparation method according to claim 3 for the water-based microorganism formulation of ecological safety, which is characterized in that step
In rapid S1, in parts by weight, the culture solution includes 8~12 parts of molasses, 90~96 parts of water.
6. the preparation method according to claim 3 for the water-based microorganism formulation of ecological safety, which is characterized in that press
According to parts by weight meter, the culture solution further includes 10~15 parts of brown sugar.
7. the preparation method according to claim 3 for the water-based microorganism formulation of ecological safety, the red vacation in marsh
Monad (Rhodopseudomonas palustris) is the strain that deposit number is ACCC10649, and the powdery finishes red ferment
Female (Pichia farinose) is the strain that deposit number is ACCC20025, the bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) it is strain that deposit number is ACCC06476, the rhodococcus erythropolis (Rhodococcus
Erythropolis) it is strain that deposit number is ACCC02667, the lactobacillus plantarum (Lactobacillus
Plantarum) it is strain that deposit number is ACCC11118, the outer red spirillum (Ectothiorhodospira of sulphur
Shaposhnikovii) it is strain that deposit number is ACCC19809.
8. the preparation method according to claim 3 for the water-based microorganism formulation of ecological safety, which is characterized in that step
In rapid S2, it is 1/4~1/5 to obtain the concentration bacterium solution that the zymocyte liquid, which is concentrated into volume, the concentration bacterium solution and described
The mass ratio of porous-starch is 1:0.5~0.7.
9. the preparation method according to claim 8 for the water-based microorganism formulation of ecological safety, which is characterized in that institute
State dextrin include mass ratio be 1:1~2 beta-cyclodextrin and maltodextrin, the concentration bacterium solution, the elbaite micro mist and
The mass ratio of the dextrin is 1:0.1~0.2:0.05~0.08.
10. the preparation method according to claim 3 for the water-based microorganism formulation of ecological safety, which is characterized in that
The mass ratio of the concentration bacterium solution and the active carbon fiber fabrics is 1:0.3~0.5.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109337693A (en) * | 2018-11-22 | 2019-02-15 | 合肥景观园林工程有限责任公司 | A kind of efficient soil pollution reparation medicament |
| CN109912047A (en) * | 2019-04-16 | 2019-06-21 | 哈尔滨工业大学 | A kind of Bioaugnentation active carbon and the method using ammonia nitrogen in active carbon removal low-temperature water source |
| CN111117938A (en) * | 2020-02-21 | 2020-05-08 | 山东海景天环保科技股份公司 | Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater |
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| CN101624234A (en) * | 2009-07-15 | 2010-01-13 | 南京农业大学 | Microorganism water treatment agent in culturing water and preparation technique thereof |
| CN102703359A (en) * | 2012-06-12 | 2012-10-03 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| CN102719381A (en) * | 2012-06-20 | 2012-10-10 | 浙江商达环保有限公司 | Composite microbial agent and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101624234A (en) * | 2009-07-15 | 2010-01-13 | 南京农业大学 | Microorganism water treatment agent in culturing water and preparation technique thereof |
| CN102703359A (en) * | 2012-06-12 | 2012-10-03 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| CN102719381A (en) * | 2012-06-20 | 2012-10-10 | 浙江商达环保有限公司 | Composite microbial agent and preparation method and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109337693A (en) * | 2018-11-22 | 2019-02-15 | 合肥景观园林工程有限责任公司 | A kind of efficient soil pollution reparation medicament |
| CN109912047A (en) * | 2019-04-16 | 2019-06-21 | 哈尔滨工业大学 | A kind of Bioaugnentation active carbon and the method using ammonia nitrogen in active carbon removal low-temperature water source |
| CN111117938A (en) * | 2020-02-21 | 2020-05-08 | 山东海景天环保科技股份公司 | Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater |
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