CN111117938A - Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater - Google Patents
Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater Download PDFInfo
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 93
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 53
- 150000001875 compounds Chemical class 0.000 title claims abstract description 48
- 239000002351 wastewater Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000007952 growth promoter Substances 0.000 claims abstract description 40
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 241000222178 Candida tropicalis Species 0.000 claims abstract description 29
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 27
- 241000235058 Komagataella pastoris Species 0.000 claims abstract description 27
- 238000001179 sorption measurement Methods 0.000 claims abstract description 24
- 241000193408 Bacillus badius Species 0.000 claims abstract description 19
- 241000605159 Nitrobacter Species 0.000 claims abstract description 15
- 244000005700 microbiome Species 0.000 claims abstract description 14
- 230000001580 bacterial effect Effects 0.000 claims description 113
- 239000007788 liquid Substances 0.000 claims description 104
- 239000010802 sludge Substances 0.000 claims description 36
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 17
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 14
- 229940099596 manganese sulfate Drugs 0.000 claims description 14
- 239000011702 manganese sulphate Substances 0.000 claims description 14
- 235000007079 manganese sulphate Nutrition 0.000 claims description 14
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 12
- 239000011790 ferrous sulphate Substances 0.000 claims description 12
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 12
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 12
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 11
- 239000004382 Amylase Substances 0.000 claims description 10
- 102000013142 Amylases Human genes 0.000 claims description 10
- 108010065511 Amylases Proteins 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- 235000019418 amylase Nutrition 0.000 claims description 10
- 238000000926 separation method Methods 0.000 claims description 9
- 229960000892 attapulgite Drugs 0.000 claims description 6
- 229910052625 palygorskite Inorganic materials 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 241000589516 Pseudomonas Species 0.000 claims description 4
- 229910021536 Zeolite Inorganic materials 0.000 claims description 4
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 235000019419 proteases Nutrition 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000010457 zeolite Substances 0.000 claims description 4
- 239000005909 Kieselgur Substances 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 241000206596 Halomonas Species 0.000 claims description 2
- 239000003463 adsorbent Substances 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 11
- 239000000243 solution Substances 0.000 description 30
- 238000000855 fermentation Methods 0.000 description 22
- 230000004151 fermentation Effects 0.000 description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 239000001963 growth medium Substances 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 239000011780 sodium chloride Substances 0.000 description 11
- 239000002609 medium Substances 0.000 description 10
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- 238000011218 seed culture Methods 0.000 description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 238000012258 culturing Methods 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- 230000001546 nitrifying effect Effects 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 230000004913 activation Effects 0.000 description 5
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 239000002068 microbial inoculum Substances 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 235000019764 Soybean Meal Nutrition 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 239000010865 sewage Substances 0.000 description 4
- 239000004455 soybean meal Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 239000002518 antifoaming agent Substances 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 239000010985 leather Substances 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 2
- 241000193410 Bacillus atrophaeus Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000003631 expected effect Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000020477 pH reduction Effects 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 241000001522 Terminalia chebula Species 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000005188 flotation Methods 0.000 description 1
- 150000004687 hexahydrates Chemical class 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000010841 municipal wastewater Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000003403 water pollutant Substances 0.000 description 1
- 239000008946 yang xin Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Mycology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
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- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention belongs to the field of microbial engineering, and particularly relates to a compound microbial agent, a preparation method thereof and a treatment method of high-salt nitrogen-containing wastewater. The compound microbial agent provided by the invention comprises mixed bacteria, a microbial growth promoter and an adsorption carrier; the mixed bacteria and the microorganism growth promoter are loaded on the adsorption carrier; the mixed bacteria comprise candida tropicalis, pichia pastoris, bacillus badius, bacillus cereus, nitrobacter and halophilus. The compound microbial agent provided by the invention can obviously improve the salinity resistance of organisms, can be used for improving the biochemical treatment effect of high-salt nitrogen-containing wastewater, and has a very wide application prospect.
Description
Technical Field
The invention belongs to the field of microbial engineering, and particularly relates to a compound microbial agent, a preparation method thereof and a treatment method of high-salt nitrogen-containing wastewater.
Background
High-salt nitrogen-containing wastewater exists in a large amount in different industries, such as leather industry, pharmaceutical industry, pickling industry and printing and dyeing industry. The existence of nitrogen pollution in the water body can not only cause the problem of drinking water supply safety, but also cause a series of problems of seasonal anoxia, eutrophication and the like of surface water bodies, so that the discharge can be carried out only by denitrification treatment.
At present, a plurality of methods for treating high-salt nitrogen-containing wastewater exist, and the biochemical treatment method is widely concerned due to the characteristics of economy, high efficiency, small environmental pollution and the like. However, too high salinity has a large impact on the conventional biochemical treatment process, mainly manifested as: 1) excessive salinity raises the osmotic pressure in the water environment, resulting in microbial wall separation; 2) too high a salt concentration may affect the biological enzyme activity secreted by the microorganism; 3) too high a salt concentration may affect the sludge settling properties.
Due to the problems, the application effect of the conventional biochemical treatment process in the treatment of the high-salt nitrogen-containing wastewater is poor. How to improve the resistance of the microorganisms to salinity and improve the biochemical treatment effect of the high-salinity nitrogen-containing wastewater is a technical problem to be solved urgently by technical personnel in the field.
Disclosure of Invention
In view of the above, the invention aims to provide a compound microbial agent, a preparation method thereof and a treatment method of high-salt nitrogen-containing wastewater.
The invention provides a compound microbial agent, which comprises mixed bacteria, a microbial growth promoter and an adsorption carrier; the mixed bacteria and the microorganism growth promoter are loaded on the adsorption carrier;
the mixed bacteria comprise candida tropicalis, pichia pastoris, bacillus badius, bacillus cereus, nitrobacter and halophilus.
Preferably, the ratio of the effective viable count of the candida tropicalis, the pichia pastoris, the bacillus chemeri, the bacillus cereus, the nitrobacter and the halophiles is (5-21): (1.6-6.6): (30-150): (150-500): (0.45-0.8): (40-100).
Preferably, the effective viable count of the compound microbial agent is 2 multiplied by 109～5×109CFU/g。
Preferably, the microbial growth promoter comprises one or more of protease, amylase, ferrous sulfate, magnesium sulfate, dipotassium hydrogen phosphate and manganese sulfate.
Preferably, the adsorption carrier comprises one or more of zeolite powder, diatomite and attapulgite.
The invention provides a preparation method of a compound microbial agent, which comprises the following steps:
a) respectively preparing candida tropicalis bacterial liquid, pichia pastoris bacterial liquid, bacillus badius bacterial liquid, bacillus cereus bacterial liquid, nitrobacter bacterial liquid and halophilous pseudomonas bacterial liquid;
b) mixing 6 bacterial liquids prepared in the step a), adding a filter aid, and performing centrifugal separation to obtain bacterial sludge;
c) and mixing the bacterial sludge, the microbial growth promoter solution and the adsorption carrier, and drying in the shade to obtain the compound microbial agent.
Preferably, in step a), the candida tropicalis bacterial liquid is prepared by fermentation of candida tropicalis liquidThe effective viable count of the mother liquor is 5 multiplied by 109～7×109CFU/mL; the effective viable count of the pichia pastoris bacterial liquid is 1.6 multiplied by 109～2.2×109CFU/mL; the effective viable count of the bacillus badius bacterial liquid is 10 multiplied by 109～30×109CFU/mL; the effective viable count of the bacillus cereus liquid is 50 multiplied by 109～100×109CFU/mL; the effective viable count of the nitrobacillus liquid is 1.5 multiplied by 107～2×107CFU/mL; the effective viable count of the halophilous unicellular bacterium liquid is 1 multiplied by 109～2×109CFU/mL。
Preferably, in the step b), the candida tropicalis bacterial liquid, the pichia pastoris bacterial liquid, the bacillus aubergiensis bacterial liquid, the bacillus cereus bacterial liquid, the nitrobacter bacterial liquid and the halophilic monad bacterial liquid are mixed according to the volume ratio of (1-3): (1-3): (3-5): (3-5): (30-40): (40-50) mixing.
Preferably, in step c), the microbial growth promoter solution comprises: 2-4 wt% of protease, 4-8 wt% of amylase, 0.03-0.05 wt% of ferrous sulfate, 0.03-0.05 wt% of magnesium sulfate, 0.3-0.5 wt% of dipotassium hydrogen phosphate and 0.02-0.03 wt% of manganese sulfate, and the solvent is water;
the water content of a mixed system formed by the bacterial sludge and the microbial growth promoter solution is 4-8% higher than that of the bacterial sludge.
The invention provides a method for treating high-salt nitrogen-containing wastewater, which is characterized in that the compound microbial agent in the technical scheme or the compound microbial agent prepared by the preparation method in the technical scheme is added into a biochemical treatment tank in the process of carrying out biochemical treatment on the wastewater.
Compared with the prior art, the invention provides a compound microbial agent, a preparation method thereof and a treatment method of high-salt nitrogen-containing wastewater. The compound microbial agent provided by the invention comprises mixed bacteria, a microbial growth promoter and an adsorption carrier; the mixed bacteria and the microorganism growth promoter are loaded on the adsorption carrier; the mixed bacteria comprise candida tropicalis, pichia pastoris, bacillus badius, bacillus cereus, nitrobacter and halophilus. The compound microbial agent provided by the invention takes halophilous unicellular bacteria and nitrobacillus as main strains, and candida tropicalis, pichia pastoris, bacillus badius and bacillus cereus as auxiliary strains, and has strong adaptability to various water qualities. Moreover, halophilic monads in the microbial inoculum can also carry out aerobic nitrification and denitrification under the condition of 5-30 g/L of NaCl, so that the total nitrogen of the high-salinity wastewater can be effectively removed. In addition, the microbial growth promoter added into the compound microbial agent provided by the invention can not only promote the improvement of the activity of the microbial agent and enhance the adaptability of the microbial agent, but also accelerate the decomposition of organic substances in water, thereby further improving the biochemical treatment effect of the saline nitrogen-containing wastewater. The compound microbial agent provided by the invention provides a proper activated sludge bacteria source for biochemical treatment of high-salt nitrogen-containing wastewater, solves the technical problem that the high-salt nitrogen-containing wastewater is difficult to biochemically treat, and has a very wide application prospect.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a compound microbial agent, which comprises mixed bacteria, a microbial growth promoter and an adsorption carrier; the mixed bacteria and the microorganism growth promoter are loaded on the adsorption carrier;
the mixed bacteria comprise candida tropicalis, pichia pastoris, bacillus badius, bacillus cereus, nitrobacter and halophilus.
The compound microbial agent provided by the invention comprises mixed bacteria, a microbial growth promoter and an adsorption carrier. The mixed bacteria are loaded on the adsorption carrier and comprise candida tropicalis, pichia pastoris, bacillus chebula, bacillus cereus, nitrobacter and halophilous monads. In the present invention, the Candida tropicalis,The ratio of the effective viable count of the pichia pastoris, the bacillus licheniformis, the bacillus cereus, the nitrobacter and the halophilous monads is preferably (5-21): (1.6-6.6): (30-150): (150-500): (0.45-0.8): (40-100), more preferably (6-13): (1.8-4): (50-120): (200-360): (0.5-0.7): (60-90), most preferably (6.2-12.4): (1.8-3.6): (60-100): (210-350): (0.6-0.7): (80-90), specifically 6.2:1.8:60:210:0.7:80 or 12.4:3.6:100:350:0.6: 90; the effective viable count of the compound microbial agent is preferably 2 x 109～5×109CFU/g, specifically 2X 109CFU/g、2.1×109CFU/g、2.2×109CFU/g、2.3×109CFU/g、2.4×109CFU/g、2.5×109CFU/g、2.6×109CFU/g、2.7×109CFU/g、2.8×109CFU/g、2.9×109CFU/g、3×109CFU/g、3.1×109CFU/g、3.2×109CFU/g、3.3×109CFU/g、3.4×109CFU/g、3.5×109CFU/g、3.6×109CFU/g、3.7×109CFU/g、3.8×109CFU/g、3.9×109CFU/g、4×109CFU/g、4.1×109CFU/g、4.2×109CFU/g、4.3×109CFU/g、4.4×109CFU/g、4.5×109CFU/g、4.6×109CFU/g、4.7×109CFU/g、4.8×109CFU/g、4.9×109CFU/g or 5X 109CFU/g。
In the present invention, the microorganism growth promoter is supported on the adsorption carrier, and preferably includes one or more of protease, amylase, ferrous sulfate, magnesium sulfate, dipotassium hydrogen phosphate, and manganese sulfate, and more preferably includes protease, amylase, ferrous sulfate, magnesium sulfate, dipotassium hydrogen phosphate, and manganese sulfate; the mass ratio of the protease, the amylase, the ferrous sulfate, the magnesium sulfate, the dipotassium hydrogen phosphate and the manganese sulfate is preferably (2-4): (4-8): (0.03-0.05): (0.03-0.05): (0.3-0.5): (0.02-0.03).
In the present invention, the adsorption carrier preferably includes one or more of zeolite powder, diatomaceous earth and attapulgite; the particle size of the adsorption carrier is preferably 50-800 meshes, and specifically can be 50 meshes, 100 meshes, 150 meshes, 200 meshes, 250 meshes, 300 meshes, 350 meshes, 400 meshes, 450 meshes, 500 meshes, 550 meshes, 600 meshes, 650 meshes, 700 meshes, 750 meshes or 800 meshes.
The compound microbial agent provided by the invention takes halophilous unicellular bacteria and nitrobacillus as main strains, and candida tropicalis, pichia pastoris, bacillus badius and bacillus cereus as auxiliary strains, and has strong adaptability to various water qualities. Moreover, halophilic monads in the microbial inoculum can also carry out aerobic nitrification and denitrification under the condition of 5-30 g/L of NaCl, so that the total nitrogen of the high-salinity wastewater can be effectively removed. In addition, the microbial growth promoter added into the compound microbial agent provided by the invention can not only promote the improvement of the activity of the microbial agent and enhance the adaptability of the microbial agent, but also accelerate the decomposition of organic substances in water, thereby further improving the biochemical treatment effect of the saline nitrogen-containing wastewater. The compound microbial agent provided by the invention provides a proper activated sludge bacteria source for biochemical treatment of high-salt nitrogen-containing wastewater, solves the technical problem that the high-salt nitrogen-containing wastewater is difficult to biochemically treat, and has a very wide application prospect.
The invention also provides a preparation method of the compound microbial agent, which comprises the following steps:
a) respectively preparing candida tropicalis bacterial liquid, pichia pastoris bacterial liquid, bacillus badius bacterial liquid, bacillus cereus bacterial liquid, nitrobacter bacterial liquid and halophilous pseudomonas bacterial liquid;
b) mixing 6 bacterial liquids prepared in the step a), adding a filter aid, and performing centrifugal separation to obtain bacterial sludge;
c) and mixing the bacterial sludge, the microbial growth promoter solution and the adsorption carrier, and drying in the shade to obtain the compound microbial agent.
In the preparation method provided by the invention, candida tropicalis bacterial liquid, pichia pastoris bacterial liquid, bacillus badius bacterial liquid, bacillus cereus bacterial liquid, nitrobacter bacterial liquid and halophilous monad bacterial liquid are respectively prepared firstly. Wherein, the candida tropicalis bacterial liquid, the pichia pastoris bacterial liquid, the bacillus aubergiensis bacterial liquid, the bacillus cereus bacterial liquid and the halophilThe halomonas solution is preferably prepared by firstly preparing a seed solution and then fermenting; the nitrifying bacillus bacterial liquid is preferably prepared by adopting an acclimation culture mode. In the present invention, the effective viable count of candida tropicalis solution of the candida tropicalis solution is preferably 5 × 109～7×109CFU/mL, specifically 5X 109CFU/mL、5.1×109CFU/mL、5.2×109CFU/mL、5.3×109CFU/mL、5.4×109CFU/mL、5.5×109CFU/mL、5.6×109CFU/mL、5.7×109CFU/mL、5.8×109CFU/mL、5.9×109CFU/mL、6×109CFU/mL、6.1×109CFU/mL、6.2×109CFU/mL、6.3×109CFU/mL、6.4×109CFU/mL、6.5×109CFU/mL、6.6×109CFU/mL、6.7×109CFU/mL、6.8×109CFU/mL、6.9×109CFU/mL or 7X 109CFU/mL; the effective viable count of the pichia pastoris bacterial liquid is preferably 1.6 multiplied by 109～2.2×109CFU/mL, specifically 1.6X 109CFU/mL、1.65×109CFU/mL、1.7×109CFU/mL、1.75×109CFU/mL、1.8×109CFU/mL、1.85×109CFU/mL、1.9×109CFU/mL、1.95×109CFU/mL、2×109CFU/mL、2.05×109CFU/mL、2.1×109CFU/mL、2.15×109CFU/mL or 2.2X 109CFU/mL; the effective viable count of the bacillus badius bacterial liquid is preferably 10 multiplied by 109～30×109CFU/mL, specifically 10X 109CFU/mL、11×109CFU/mL、12×109CFU/mL、13×109CFU/mL、14×109CFU/mL、15×109CFU/mL、16×109CFU/mL、17×109CFU/mL、18×109CFU/mL、19×109CFU/mL、20×109CFU/mL、21×109CFU/mL、22×109CFU/mL、23×109CFU/mL、24×109CFU/mL、25×109CFU/mL、26×109CFU/mL、27×109CFU/mL、28×109CFU/mL、29×109CFU/mL or 30X 109CFU/mL; the effective viable count of the bacillus cereus liquid is preferably 50 multiplied by 109～100×109CFU/mL, specifically 50X 109CFU/mL、55×109CFU/mL、60×109CFU/mL、65×109CFU/mL、70×109CFU/mL、75×109CFU/mL、80×109CFU/mL、85×109CFU/mL、90×109CFU/mL、95×109CFU/mL or 100X 109CFU/mL; the effective viable count of the nitrifying bacillus bacterial liquid is preferably 1.5 multiplied by 107～2×107CFU/mL, specifically 1.5X 107CFU/mL、1.55×107CFU/mL、1.6×107CFU/mL、1.65×107CFU/mL、1.7×107CFU/mL、1.75×107CFU/mL、1.8×107CFU/mL、1.85×107CFU/mL、1.9×107CFU/mL、1.95×107CFU/mL or 2X 107CFU/mL; the effective viable count of the halophilous pseudomonas bacterial liquid is preferably 1 × 109～2×109CFU/mL, specifically 1X 109CFU/mL、1.1×109CFU/mL、1.2×109CFU/mL、1.3×109CFU/mL、1.4×109CFU/mL、1.5×109CFU/mL、1.6×109CFU/mL、1.7×109CFU/mL、1.8×109CFU/mL、1.9×109CFU/mL or 2X 109CFU/mL。
In the preparation method provided by the present invention, after the 6 kinds of bacterial liquids are prepared, the 6 kinds of bacterial liquids are mixed. The volume ratio of the candida tropicalis bacterial liquid, the pichia pastoris bacterial liquid, the bacillus aubergiensis bacterial liquid, the bacillus cereus bacterial liquid, the nitrobacter bacterial liquid and the halophilic unicellular bacterial liquid is preferably (1-3): (1-3): (3-5): (3-5): (30-40): (40-50), more preferably (1-2): (1-2): (3-5): (3-5): (30-35): (40-45).
In the preparation method provided by the invention, 6 bacterial liquids are uniformly mixed, then a filter aid is added into the obtained mixed bacterial liquid, and then centrifugal separation is carried out. Wherein the filter aid includes, but is not limited to, attapulgite and/or diatomaceous earth; the mass/volume ratio of the filter aid to the mixed bacterial liquid is preferably (5-10) g:100mL, specifically 5g:100mL, 5.5g:100mL, 6g:100mL, 6.5g:100mL, 7g:100mL, 7.5g:100mL, 8g:100mL, 8.5g:100mL, 9g:100mL, 9.5g:100mL, or 10g:100 mL; the rotation speed of the centrifugal separation is preferably 4000-4500 r/min, and specifically can be 4000r/min, 4050r/min, 4100r/min, 4150r/min, 4200r/min, 4250r/min, 4300r/min, 4350r/min, 4400r/min, 4450r/min or 4500 r/min; the time of the centrifugal separation is preferably 30-60 min, and specifically can be 30min, 35min, 40min, 45min, 50min, 55min or 60 min. And after the centrifugal separation is finished, obtaining bacterial sludge. In the present invention, the moisture content of the bacterial sludge is preferably 20-35%, and specifically may be 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, or 35%.
In the preparation method provided by the invention, after the bacterial sludge is obtained, the bacterial sludge, the microbial growth promoter solution and the adsorption carrier are mixed. Wherein, the microorganism growth promoter solution consists of a microorganism growth promoter and a solvent, and the components of the microorganism growth promoter are introduced above and are not described again; the solvent is preferably water. In one embodiment provided by the present invention, the protease content of the microorganism growth promoter solution is preferably 2 to 4 wt%, and specifically may be 2 wt%, 2.5 wt%, 3 wt%, 3.5 wt%, or 4 wt%; the amylase content of the microbial growth promoter solution is preferably 2-4 wt%, and specifically can be 2 wt%, 2.5 wt%, 3 wt%, 3.5 wt% or 4 wt%; the content of ferrous sulfate in the microbial growth promoter solution is preferably 0.03-0.05 wt%, and specifically can be 0.03 wt%, 0.035 wt%, 0.04 wt%, 0.045 wt% or 0.05 wt%; the content of magnesium sulfate in the microbial growth promoter solution is preferably 0.03-0.05 wt%, and specifically can be 0.03 wt%, 0.035 wt%, 0.04 wt%, 0.045 wt% or 0.05 wt%; the content of dipotassium hydrogen phosphate in the microbial growth promoter solution is preferably 0.3-0.5 wt%, and specifically can be 0.3 wt%, 0.35 wt%, 0.4 wt%, 0.45 wt% or 0.5 wt%; the content of manganese sulfate in the microbial growth promoter solution is preferably 0.02-0.03 wt%, and specifically can be 0.02 wt%, 0.025 wt% or 0.03 wt%. In the invention, the amount of the microbial growth promoter solution is determined according to the mass and the water content of the bacterial sludge, and after the bacterial sludge and the microbial growth promoter solution are mixed, the water content of a mixed system formed by the bacterial sludge and the microbial growth promoter solution is preferably 4-8% higher than that of the bacterial sludge, and specifically can be 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5% or 8%. In the present invention, the amount of the adsorption carrier to be used is determined according to the mass of the mixed system composed of the bacterial sludge and the microbial growth promoter solution, and the ratio of the adsorption carrier to the total mass of the bacterial sludge and the microbial growth promoter solution is preferably 1: (0.8-1), specifically 1:0.8, 1:0.81, 1:0.82, 1:0.83, 1:0.84, 1:0.85, 1:0.86, 1:0.87, 1:0.88, 1:0.89, 1:0.9, 1:0.91, 1:0.92, 1:0.93, 1:0.94, 1:0.95, 1:0.96, 1:0.97, 1:0.98, 1:0.99 or 1: 1. In the present invention, the mixing is preferably performed by mixing the bacterial sludge and the microorganism growth promoter solution, and then mixing the mixed solution and the adsorption carrier. And after the bacterial sludge, the microbial growth promoter solution and the adsorption carrier are uniformly mixed, drying in the shade to obtain the compound microbial agent provided by the invention.
The compound microbial agent prepared by the invention takes halophilous unicellular bacteria and nitrobacillus as main strains, and is supplemented with candida tropicalis, pichia pastoris, bacillus badius and bacillus cereus, so that the compound microbial agent has strong adaptability to various water qualities. Moreover, halophilic monads in the microbial inoculum can also carry out aerobic nitrification and denitrification under the condition of 5-30 g/L of NaCl, so that the total nitrogen of the high-salinity wastewater can be effectively removed. In addition, the microbial growth promoter added in the preparation process of the compound microbial inoculant can promote the activity of the inoculant to be improved, enhance the adaptability of the inoculant and accelerate the decomposition of organic substances in water, so that the biochemical treatment effect of the salt-containing nitrogen-containing wastewater is further improved. The compound microbial agent prepared by the invention provides a proper activated sludge bacteria source for biochemical treatment of high-salt nitrogen-containing wastewater, solves the technical problem that the high-salt nitrogen-containing wastewater is difficult to biochemically treat, and has a very wide application prospect.
The invention also provides a treatment method of the high-salt nitrogen-containing wastewater, which comprises the following steps:
performing biochemical treatment on the wastewater in a biochemical treatment tank to obtain treated wastewater;
in the biochemical treatment process, the compound microbial agent in the technical scheme or the compound microbial agent prepared by the preparation method in the technical scheme is added into the biochemical treatment tank.
In the treatment method provided by the invention, the biochemical treatment tank is preferably an aerobic tank; the adding amount of the compound microbial agent is preferably 1-5 wt% of the mass of the wastewater, and specifically can be 1 wt%, 1.5 wt%, 2 wt%, 2.5 wt%, 3 wt%, 3.5 wt%, 4 wt%, 4.5 wt% or 5 wt%; the dissolved oxygen concentration of the aerobic pool is preferably controlled to be 1-8 mg/L, and specifically can be 1mg/L, 2mg/L, 3mg/L, 4mg/L, 5mg/L, 6mg/L, 7mg/L or 8 mg/L; the sludge concentration (MLSS) of the aerobic tank is preferably controlled to be 3-10 g/L, and specifically can be 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, 9g/L or 10 g/L; the sludge reflux ratio of the aerobic tank is preferably controlled to be 100-200%, and specifically can be 100%, 110%, 120%, 130%, 140%, 150%, 160%, 170%, 180%, 190% or 200%.
The treatment method provided by the invention adds the compound microbial agent in the biochemical treatment process of the high-salt nitrogen-containing wastewater, obviously improves the resistance of the microorganisms to the salinity of the wastewater, and improves the biochemical treatment effect of the high-salt nitrogen-containing wastewater.
For the sake of clarity, the following examples are given in detail.
Example 1
And (4) preparing each microbial liquid.
Except that the nitrifying bacillus adopts a domestication culture mode to prepare a bacterial liquid, other 5 microbial strains obtain the bacterial liquid according to a seed liquid preparation-fermentation mode.
(1) Preparing candida tropicalis bacterial liquid:
(1.1) seed liquid preparation: transferring the Candida tropicalis strain stored at the temperature of 4 ℃ to the temperature of 25 ℃ for activation for 4 hours; then inoculating the seeds into 500mL of seed culture medium, setting the culture temperature at 24 ℃, and the rotating speed of a shaking table at 200r/min, and culturing for 16h to obtain seed liquid.
In the step (1.1), the composition of the seed culture medium by weight (w/w) is as follows: 2% of glucose, 1% of yeast powder, 2% of peptone and the balance of water; sterilizing at 121 deg.C under 0.15Mpa for 20 min.
(1.2) fermentation: inoculating the mixture into a fermentation medium according to the inoculation amount of 1-5 percent of the volume percentage, wherein the culture temperature is 28 ℃, the rotating speed of a shaking table is 120r/min, the culture time is 16h, and the effective viable count is 6.2 multiplied by 109CFU/mL fermentation broth.
In the step (1.2), the composition of the fermentation medium is as follows by weight (w/w): 1% of glucose, 3% of soybean meal, 0.3% of ammonium nitrate, 0.5% of yeast powder, 0.05% of magnesium sulfate, 0.1% of potassium dihydrogen phosphate, 0.05% of dipotassium hydrogen phosphate, 0.01% of manganese sulfate and the balance of water; sterilizing at 121 deg.C under 0.15Mpa for 20 min.
(2) Preparing a pichia pastoris bacterial liquid:
(2.1) seed liquid preparation: transferring the Pichia pastoris strain stored at 4 ℃ to 25 ℃ for activation for 4 h; then inoculating the seeds into 500mL of seed culture medium, setting the culture temperature at 32 ℃, and the rotating speed of a shaking table at 200r/min, and culturing for 25h to obtain seed liquid.
In the step (2.1), the seed culture medium comprises the following components: 10g/L yeast extract, 20g/L peptone and 20g/L glucose; sterilizing at 121 deg.C under 0.15Mpa for 20 min.
(2.2) fermentation: inoculating into fermentation medium at an inoculation amount of 5% by volume at a culture temperature of 30 deg.C, a rotation speed of 150r/min, a tank pressure of 0.05MPa, and a culture time of 24h to obtain effective viable bacteria count of 1.8 × 109CFU/mL fermentation broth.
In the step (2.2), the fermentation medium comprises the following components: 30g/L of glucose, 21g/L of soybean meal and NH4Cl 2.2g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1.1g/L, dipotassium hydrogen phosphate 0.44g/L, manganese sulfate 0.1g/L and defoaming agent 1 ml/L.
(3) Preparing a bacillus badius bacterial solution:
(3.1) seed liquid preparation: transferring the bacillus badius strain preserved at 4 ℃ to 25 ℃ for activation for 4 h; then inoculating the seeds into 500mL of seed culture medium, setting the culture temperature at 30 ℃, and the rotating speed of a shaking table at 180r/min, and culturing for 20h to obtain seed liquid.
In the step (3.1), the seed culture medium comprises the following components: 10g/L yeast extract, 20g/L peptone, 10g/L NaCl; sterilizing at 121 deg.C under 0.15Mpa for 20 min.
(3.2) fermentation: inoculating in fermentation medium at an inoculation amount of 3% by volume at a culture temperature of 30 deg.C, a rotation speed of 200r/min, a tank pressure of 0.05MPa, and a culture time of 45h to obtain effective viable bacteria count of 20 × 109CFU/mL fermentation broth.
In the step (3.2), the fermentation medium comprises the following components: glucose 40g/L, (NH)4)2SO42g/L, 20g/L of soybean meal, 5g/L of sodium chloride, 0.1g/L of magnesium sulfate, 1g/L of monopotassium phosphate, 0.04g/L of ferrous sulfate, 0.3g/L of calcium chloride, 0.3g/L of manganese sulfate and 1ml/L of defoaming agent.
(4) Preparing a bacillus cereus liquid:
(4.1) seed liquid preparation: transferring the bacillus cereus strain preserved at the temperature of 4 ℃ to the temperature of 25 ℃ for activation for 4 hours; then inoculating the seeds into 500mL of seed culture medium, setting the culture temperature at 30 ℃, and the rotating speed of a shaking table at 180r/min, and culturing for 25h to obtain seed liquid.
In the step (4.1), the seed culture medium comprises the following components: 10g/L yeast extract, 20g/L peptone, 10g/L NaCl; sterilizing at 121 deg.C under 0.15Mpa for 20 min.
(4.2) fermentation: inoculating into fermentation medium at an inoculation amount of 1% by volume, culturing at 30 deg.C, rotating speed of 200r/min, tank pressure of 0.05MPa, and culturing for 50h to obtain effective viable bacteria count of 70 × 109CFU/mL fermentation broth.
In the step (4.2), the fermentation medium comprises the following components: 1.0% of glucose, 1.0% of soybean meal, 0.2% of ammonium nitrate, 0.02% of manganese sulfate, 0.05% of magnesium sulfate, 0.03% of sodium chloride, 0.005% of ferrous sulfate, 0.02% of calcium chloride, 7.2% of pH and 0.1% of defoaming agent.
(5) Preparing a nitrifying bacillus bacterial liquid:
taking viable bacteria concentration of 2 × 107Adding the CFU/mL nitrobacillus seed solution into 5L culture medium with ammonia nitrogen concentration of 50mg/L according to the volume ratio of 20%In the solution, detecting the concentration of the residual ammonia nitrogen in the culture solution every 12h, replenishing the ammonia nitrogen when the concentration of the residual ammonia nitrogen is reduced to be below 5mg/L, sequentially increasing the concentration of the ammonia nitrogen, increasing the concentration by 50mg/L each time, completing the culture of the nitrobacteria when the concentration of the ammonia nitrogen reaches 200mg/L and the concentration of the ammonia nitrogen is reduced to be below 5mg/L in 12h, and obtaining the viable bacteria concentration of 2 multiplied by 107CFU/mL culture medium.
(6) Preparing halophilic monad bacterial liquid:
(6.1) seed liquid preparation: transferring the halophilous monad strain stored at the temperature of 4 ℃ to the temperature of 25 ℃ for activation for 4 hours; then inoculating the seeds into 500mL of seed culture medium, setting the culture temperature at 30 ℃, and the rotating speed of a shaking table at 150r/min, and culturing for 25h to obtain seed liquid.
In the step (6.1), the seed culture medium comprises the following components: 10g/L yeast extract, 20g/L peptone, 10g/L NaCl; sterilizing at 121 deg.C under 0.15Mpa for 20 min.
(6.2) fermentation: inoculating in fermentation medium at 30 deg.C, rotation speed of 180r/min, tank pressure of 0.05MPa, and culture time of 35h to obtain effective viable bacteria number of 2 × 109CFU/mL fermentation broth.
In the step (6.2), the fermentation medium comprises the following components: 7g/L of hexahydrate and magnesium chloride, 9.6g of magnesium sulfate heptahydrate, 0.36g/L of calcium chloride, 2.0g/L of potassium chloride, 0.06g/L of sodium bicarbonate, 0.026g/L of sodium bromide, 5.0g/L of peptone, 10g/L of yeast extract, 1.0g/L of glucose and 30g/L of sodium chloride.
Example 2
Preparing a compound microbial agent:
(1) the 6 inoculum prepared in example 1 was mixed according to the candida tropicalis inoculum: a pichia pastoris bacterial liquid: b, bacillus atrophaeus bacterial liquid: bacillus cereus liquid: nitrifying bacillus bacterial liquid: uniformly mixing halophilic monad bacterial liquid in a volume ratio of 1:1:3:3:35:40 to obtain mixed bacterial liquid; then adding diatomite into the mixed bacterial liquid according to the mass/volume ratio of 5g/100 mL; and then placing the mixture in a three-foot centrifuge for centrifugation at 4000r/min for 40min, collecting bacterial sludge and heaving, and detecting that the water content is 25%.
(2) Preparing a solution according to the mass ratio of 2% of protease, 4% of amylase, 0.03% of ferrous sulfate, 0.05% of magnesium sulfate, 0.3% of dipotassium hydrogen phosphate, 0.02% of manganese sulfate and the balance of water; and (2) adding the mixture into the bacterial sludge prepared in the step (1) and uniformly stirring to obtain a bacterial sludge mixture with the water content of 30%.
(3) Mixing zeolite powder (100-200 meshes) and the bacterial sludge mixture obtained in the step (2) according to a mass ratio of 1:0.8, mixing uniformly, airing under the natural ventilation and cool condition, and avoiding direct irradiation of sunlight to obtain the compound microbial agent.
The effective viable count contained in the compound microbial agent is measured to reach 3.0 multiplied by 10 through plate counting9CFU/g。
Example 3
Preparing a compound microbial agent:
(1) the 6 inoculum prepared in example 1 was mixed according to the candida tropicalis inoculum: a pichia pastoris bacterial liquid: b, bacillus atrophaeus bacterial liquid: bacillus cereus liquid: nitrifying bacillus bacterial liquid: uniformly mixing halophilic monad bacterial liquid in a volume ratio of 2:2:5:5:30:45 to obtain mixed bacterial liquid; then adding attapulgite into the mixed bacterial liquid according to the mass/volume ratio of 5g/100 mL; and then placing the mixture in a three-foot centrifuge for centrifugation at 4000r/min for 40min, collecting bacterial sludge and heaving, and detecting that the water content is 28%.
(2) Preparing a solution according to the mass ratio of 3% of protease, 2% of amylase, 0.05% of ferrous sulfate, 0.05% of magnesium sulfate, 0.5% of dipotassium hydrogen phosphate, 0.03% of manganese sulfate and the balance of water; and (2) adding the mixture into the bacterial sludge prepared in the step (1) and uniformly stirring to obtain a bacterial sludge mixture with the water content of 35%.
(3) Mixing attapulgite (100-300 meshes) and the bacterial sludge mixture obtained in the step (2) according to a mass ratio of 1:1, uniformly mixing, airing under natural ventilation and cool conditions, and avoiding direct irradiation of sunlight to obtain the compound microbial agent.
Counting by flat plate to obtain effective viable count of 4.0 × 109CFU/g。
Example 4
The application of the compound microbial agent in the treatment of high-salt nitrogen-containing wastewater of a certain leather factory is as follows:
the water inlet indexes of a sewage treatment system of certain Shandong Yangxin leather factory are as follows: 15.5 wt% of total salt, 650mg/L of total nitrogen and more than 10000mg/L of COD, and the production wastewater is sequentially treated in a regulating tank, a flocculation sedimentation tank, a hydrolysis acidification tank, an anoxic tank, an aerobic tank and a secondary sedimentation tank to obtain the treated wastewater. Because the salinity is higher, the COD of the treated wastewater is more than 450mg/L, the ammonia nitrogen is more than 100mg/L, and the total nitrogen is more than 200mg/L, which does not meet the discharge standard of water pollutants in the leather-making and fur processing industry.
The sewage treatment system was adjusted by using the complex microbial inoculum prepared in example 2, and the operation parameters of each structure were controlled as shown in the following table:
the composite microbial agent is added into the aerobic pool according to the addition of 2wt per thousand, and the total nitrogen of the treated wastewater is lower than 70mg/L after 10 days of debugging, the removal rate reaches 99.2 percent, the COD is lower than 200mg/L, the removal rate reaches 99.8 percent, and the expected effect is achieved. After three months of continuous operation, the device can ensure that the effluent reaches the standard and is discharged.
Example 5
The application of the compound microbial agent in the treatment of high-salt nitrogen-containing wastewater of a certain petrochemical company is as follows:
the water inlet indexes of the sewage treatment system of a certain petrochemical company in Shandong Binzhou are as follows: 21.8 wt% of total salt, 250mg/L of total nitrogen and more than 8000/L of COD, and the production wastewater is sequentially treated in a homogenizing tank, an oil separation tank, an air flotation tank, a hydrolysis acidification tank, an anoxic tank, an aerobic tank and a secondary sedimentation tank to obtain the treated wastewater. Due to the influence of salinity, effluent indexes of COD (chemical oxygen demand) 80mg/L, ammonia nitrogen 10mg/L and total nitrogen 20mg/L do not meet the discharge standard of pollutants for municipal wastewater treatment plants.
The sewage treatment system was adjusted by using the complex microbial inoculum prepared in example 3, and the operation parameters of each structure were controlled as shown in the following table:
the composite microbial agent is added into the aerobic pool according to the adding amount of 3wt per thousand, and after 8d of debugging, the total nitrogen of the treated wastewater is lower than 15mg/L, the removal rate reaches 99.2 percent, the COD reaches lower than 50mg/L, the removal rate reaches 99.8 percent, and the expected effect is achieved. After three months of continuous operation, the device can ensure that the effluent reaches the standard and is discharged.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A compound microbial agent comprises mixed bacteria, a microbial growth promoter and an adsorption carrier; the mixed bacteria and the microorganism growth promoter are loaded on the adsorption carrier;
the mixed bacteria comprise candida tropicalis, pichia pastoris, bacillus badius, bacillus cereus, nitrobacter and halophilus.
2. The compound microbial agent according to claim 1, wherein the ratio of the effective viable count of Candida tropicalis, Pichia pastoris, Bacillus badius, Bacillus cereus, Nitrobacter and Halomonas halophilus is (5-21): (1.6-6.6): (30-150): (150-500): (0.45-0.8): (40-100).
3. The complex microbial inoculant according to claim 1, wherein the effective viable count of the complex microbial inoculant is 2 x 109～5×109CFU/g。
4. The complex microbial inoculant according to claim 1, wherein the microbial growth promoter comprises one or more of protease, amylase, ferrous sulfate, magnesium sulfate, dipotassium hydrogen phosphate and manganese sulfate.
5. The composite microbial inoculant according to claim 1, wherein said adsorbent carrier comprises one or more of zeolite powder, diatomaceous earth and attapulgite.
6. A preparation method of a compound microbial agent comprises the following steps:
a) respectively preparing candida tropicalis bacterial liquid, pichia pastoris bacterial liquid, bacillus badius bacterial liquid, bacillus cereus bacterial liquid, nitrobacter bacterial liquid and halophilous pseudomonas bacterial liquid;
b) mixing 6 bacterial liquids prepared in the step a), adding a filter aid, and performing centrifugal separation to obtain bacterial sludge;
c) and mixing the bacterial sludge, the microbial growth promoter solution and the adsorption carrier, and drying in the shade to obtain the compound microbial agent.
7. The method according to claim 6, wherein the effective viable count of the Candida tropicalis solution in step a) is 5X 109～7×109CFU/mL; the effective viable count of the pichia pastoris bacterial liquid is 1.6 multiplied by 109～2.2×109CFU/mL; the effective viable count of the bacillus badius bacterial liquid is 10 multiplied by 109～30×109CFU/mL; the effective viable count of the bacillus cereus liquid is 50 multiplied by 109～100×109CFU/mL; the effective viable count of the nitrobacillus liquid is 1.5 multiplied by 107～2×107CFU/mL; the effective viable count of the halophilous unicellular bacterium liquid is 1 multiplied by 109～2×109CFU/mL。
8. The preparation method according to claim 7, wherein in the step b), the Candida tropicalis bacterial liquid, the Pichia pastoris bacterial liquid, the Bacillus badius bacterial liquid, the Bacillus cereus bacterial liquid, the Bacillus nitrificans bacterial liquid and the halophilous unicellular bacterial liquid are mixed according to a volume ratio of (1-3): (1-3): (3-5): (3-5): (30-40): (40-50) mixing.
9. The method of claim 6, wherein in step c), the microbial growth promoting solution comprises: 2-4 wt% of protease, 4-8 wt% of amylase, 0.03-0.05 wt% of ferrous sulfate, 0.03-0.05 wt% of magnesium sulfate, 0.3-0.5 wt% of dipotassium hydrogen phosphate and 0.02-0.03 wt% of manganese sulfate, and the solvent is water;
the water content of a mixed system formed by the bacterial sludge and the microbial growth promoter solution is 4-8% higher than that of the bacterial sludge.
10. A method for treating high-salt nitrogen-containing wastewater is characterized in that the compound microbial agent as defined in any one of claims 1-5 or the compound microbial agent prepared by the preparation method as defined in any one of claims 6-9 is added into a biochemical treatment tank in the process of performing biochemical treatment on the wastewater.
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