CN109868233A - A kind of efficient acid water special bacteria agent and its application method - Google Patents
A kind of efficient acid water special bacteria agent and its application method Download PDFInfo
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Abstract
The present invention relates to technical field of sewage, especially a kind of efficient acid water special bacteria agent and its application method.The efficient acid water special bacteria agent is made of the bacterium powder of three plants of bacterium, respectively candida tropicalis, and bacterial strain code is YJY18-08, and deposit number is CGMCC NO.16156；Bacillus cereus, bacterial strain code are YJY18-09；Its deposit number is CGMCC NO.16157；Nitrobacter winogradskyi, bacterial strain code are Y3-2, and deposit number is CCTCC NO:M2014203.The efficient acid water dedicated processes microbial inoculum is put into activated sludge, high load capacity can be effectively resistant to or inhibit the impact of harmful substance, improve the removal efficiency of former biochemical system volatile phenol, sulfide, ammonia nitrogen, materialized pretreatment process can be saved again, save cost, sewage after treatment reaches comprehensive pool discharge standard, without side-effects to operator.
Description
Technical field
The present invention relates to technical field of sewage, especially a kind of efficient acid water special bacteria agent and its application method.
Background technique
With the rapid development of the industries such as petrochemical industry, plastics, coking, the generation of all kinds of acid waters also accordingly increases.Petroleum and
There are sulfur-containing compounds and nitrogenous compound in its product, in Atmospheric vacuum, catalytic cracking, thermal cracking, delayed coking, reform pre-add
Hydrogen, hydrofinishing are hydrocracked etc. in process, these compounds can pass through Pintsch process, catalytic cracking, catalytic hydrogenation etc.
Reaction generates H2S and NH3And enter in product stream, these product streams pass through condensation dehydration or washing process, i.e. generation sulfur-bearing
Ammonia-containing water, is commonly called as sulfur-containing waste water or acid water, and specific drainage arrangement has atmospheric and vacuum distillation tower top separator, catalytic cracking point
Evaporate overhead separator, catalytic cracking compression rich gas drain sump, coking fractional distillation column top separator and oil hydrogenation cracking plus hydrogen
High and low pressure separator of purification etc., outside the vulcanisation hydrogen of pollutant and ammonia nitrogen in acid water, there are also oil, phenol, cyanogen etc..Acid water
Middle sulfide and ammonia density are different with processing unit (plant) with crude oil type and change, and generally speaking, crude oil sulfur-bearing nitrogen content is higher, acid
Property Sulfide in water and ammonia density are higher；Divide by processing unit (plant), acid Sulfide in water and ammonia density are from high to low substantially are as follows:
It is hydrocracked, hydrofinishing > coking, catalytic cracking > normal, decompression.Therefore, someone will be hydrocracked, hydrofinishing, reform pre-add
The acid water of hydrogen production device discharge is divided into high-concentration acidic wastewater water, and the acid water that catalytic cracking, coking plant discharge is divided into
The acid water that normal, decompressor discharges is divided into low concentration acid water by concentration acid water.
Small water, the acid water process of low concentration develop to personalization technology, such as high-effective microorganism technology, chemistry
Oxidation sweetening-stripping deamination technology, chemical oxidation desulfurization-bio-denitrification technology etc., to mitigate petrochemical industry stripper plant operating load
The microbial inoculum of phenol, ammonia nitrogen, sulfide, COD drop by screening in high and at high cost problem, this project, construct biological flora, obtain
The microbial inoculum of efficient process acid water makes the pollutant in waste water be converted into metabolic end-product by the metabolic activity of microorganism
CO2With the harmless products such as water, and it is purified waste water.Simultaneously have low energy consumption, running expense is low, simple process, without secondary
The remarkable advantages such as pollution.
Summary of the invention
The purpose of the present invention is in view of the above problems, provide a kind of efficient acid water special bacteria agent and its application
Method.The microbial inoculum be according to volatile phenol, sulfide in low concentration acid water, ammonia nitrogen index is relatively low and researches and develops.To department of biochemistry
Efficient acid water dedicated processes microbial inoculum is added in system, for improving the removal effect of former biochemical system volatile phenol, ammonia nitrogen, sulfide
Rate is effectively resistant to the impact of load or inhibiting substances, guarantees that being discharged each index reaches discharge standard.
A kind of efficient acid water special bacteria agent of the present invention, is made of the bacterium powder of three plants of bacterium, respectively the false silk in the torrid zone
Yeast, bacterial strain code are YJY18-08, and deposit number is CGMCC NO.16156；Bacillus cereus, bacterial strain code are
YJY18-09；Its deposit number is CGMCC NO.16157；Nitrobacter winogradskyi, bacterial strain code are Y3-2, deposit number CCTCC
NO:M2014203.
The bacterium amount of the bacterium powder is single bacterium 5 × 107~5 × 1010。
The microbial inoculum is carried out respectively by Candida tropicalis powder, Nitrobacter winogradskyi bacterium powder and bacillus cereus bacterium powder
After activation, composition is compounded according to the volume ratio of 1~3:1:1~3.
In the present invention, bacillus cereus degradation volatile phenol, Nitrobacter winogradskyi degradation of ammonia nitrogen, candida tropicalis degradation
Sulfide, the present invention remove the toxic action of sulphide removal, bacillus cereus and Nitrobacter winogradskyi using candida tropicalis
It plays a role, removes volatile phenol and ammonia nitrogen.
The preparation method of the efficient acid water special bacteria agent, including following operating procedure:
(1) fermented and cultured: after candida tropicalis, Nitrobacter winogradskyi and bacillus cereus are inoculated in sterilizing respectively
Fermented and cultured obtains respective bacterium solution in fermentation medium；Under conditions of temperature is 20-40 DEG C, in fermentation tank culture to dissolved oxygen
It rises, pH decline；
(2) it prepares bacterium powder: after fermentation liquid is centrifuged, adding auxiliary materials and mixing, solid bacterium powder is obtained after drying；
(3) it mixes: above-mentioned bacterium powder is mixed according to mass ratio to get efficient acid water special bacteria agent is arrived.
More specifically steps are as follows:
1) actication of culture: on aseptic operating platform, the Candida tropicalis powder, the Nitrobacter winogradskyi that take 1~5 μ L to freeze
Bacterium powder and bacillus cereus are in the test tube containing LB liquid medium, and 28 DEG C~32 DEG C, 130~180rpm cultivates 18-
24h；
2) prepared by seed: on aseptic operating platform, the strain of activation being transferred to the triangle containing sterilized liquid LB culture medium
In bottle, packing 200mL culture medium in every triangular flask, test tube strains one triangular flask of inoculation of an activation, then 28 DEG C~
32 DEG C, 130~180rpm cultivates 18-24h；
3) inoculation fermentation tank: fermentation tank, pipeline, air filter are subjected to sky first and disappeared, 121 DEG C, sky disappears
30min injects culture medium after temperature reduction, then disappear in fact to culture medium, 121 DEG C, disappear 30min in fact；It is down to temperature
It is inoculated at 28 DEG C~32 DEG C；
4) fermentation condition: inoculum concentration v/v 5%~10%；28 DEG C~32 DEG C of cultivation temperature；PH:6.8-7.0；It is initial to turn
Speed: 200rpm；Fermentor pressure: 0.05Mpa；Ventilatory capacity ratio: 1:1；Fermentation period: 16~for 24 hours；
5) prepared by bacterium powder: after fermentation, addition diatomite, cornstarch adsorb thallus, dry later, crush and can obtain
To corresponding bacterium powder.
The culture medium for strain isolation is LB solid medium, culture medium prescription are as follows: peptone 10g/L,
NaCl10g/L, yeast extract 5g/L, pH 7.0-7.2, solvent is water.
The fermentation medium of the candida tropicalis forms are as follows: by weight are as follows: 1~2 part of peptone, 0.5-1 parts
Yeast extract, 1~3 part of sodium chloride, 94~97.5 parts of softened waters；
The fermentation medium of the bacillus cereus forms are as follows: by weight are as follows: 1-1.5 parts of glucose, 1-1.5 parts
Dregs of beans, 0.2-0.3 divide ammonium nitrate, 0.01-0.03 parts of manganese sulfates, 0.03-0.07 parts of magnesium sulfate, 0.02-0.05 parts of sodium chloride,
0.02-0.05 parts of calcium chloride, 0.003-0.005 parts of ferrous sulfate, 96.5-97.7 parts of softened waters；
The fermentation medium of the Nitrobacter winogradskyi forms are as follows: by weight are as follows: 1-3 parts of ammonium sulfate, 1-1.5 parts sub-
Sodium nitrate, 0.2-0.3 divide sodium dihydrogen phosphate, 0.1-1.0 parts of dipotassium hydrogen phosphates, 0.03-0.07 parts of magnesium sulfate, 3.0-5.0 parts of carbon
Sour calcium, 90-95.5 parts of softened waters.
The efficient acid water special bacteria agent of above-mentioned acquisition is used for the processing of acid water, detailed process are as follows:
Nutritive salt is added in oxytropism water, is mixed with activated sludge, system pH is adjusted to 6.0-7.0 with sulfuric acid, is added high
Imitate acid water special bacteria agent, aeration culture 24-30h.
The nutrient salt solution is dipotassium hydrogen phosphate 0.05-0.4g/L, potassium dihydrogen phosphate 0.05-0.4g/L, magnesium sulfate
0.0125-0.1g/L, ammonium chloride 0.125-1.0g/L, sodium bicarbonate 0.031-0.25g/L, trace element solution 10-20mL, water
Complement to 1L.
The trace element solution group becomes ferrous sulfate 0.0625-0.5g/L, manganese sulfate 0.0125-0.1g/L, chlorine
Change zinc 0.025-0.2g/L, calcium chloride 0.0125-0.1g/L, water complements to 1L.
The additional amount of nutrient salt solution is the 0.001-0.05% of acid water quality；Activated sludge is added in every liter of acid water
5-10g。
Above-mentioned efficient acid water special bacteria agent is applied to processing system, drop phenol ability is greater than 500mg/ (L.d), desulfurization energy
Power 300mg/ (L.d), drop ammonia nitrogen ability reach 50mg/ (L.d), are applied to former processing system, to the adaptable of system, effect
Stablize.Meanwhile high-efficiency strain tolerance is strong, has wide range of applications.
Present invention has the advantage that
(1) inoculating microbe Candida tropicalis powder, Nitrobacter winogradskyi bacterium powder and bacillus cereus, enhancing are introduced
Microbial activity and population advantage, improve the removal efficiency of former biochemical system ammonia nitrogen, sulfide, are effectively resistant to load or suppression
The impact of substance processed guarantees that being discharged each index reaches discharge standard.
(2) desulfurization, drop ammonia nitrogen are high-efficient, and every batch of aerobic system is discharged sulfide < 1mg/L, and ammonia nitrogen < 5mg/L reaches
The standard into comprehensive pool is arrived.
(3) simplify operation, save human and material resources, reduce equipment and use pressure.
(4) energy-saving and environmental protection, nontoxic.
(5) process flow is short, and technological operation is highly-safe.
Preservation explanation
The preservation time: on July 25th, 2018
Depositary institution's title: Chinese microorganism strain preservation conservator's common micro-organisms center
Deposit number: CGMCC No.16156
Depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology, the Chinese Academy of Sciences
Classification naming: candida tropicalis Candida tropicalis
The preservation time: on July 25th, 2018
Depositary institution's title: Chinese microorganism strain preservation conservator's common micro-organisms center
Deposit number: CGMCC No.16157
Depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology, the Chinese Academy of Sciences
Classification naming: bacillus cereus Bacillus cereus
Specific embodiment
Invention is described further combined with specific embodiments below.
Embodiment 1
A kind of efficient acid water special bacteria agent, is made of, respectively candida tropicalis, bacterial strain code the bacterium powder of three plants of bacterium
For YJY18-08, deposit number is CGMCC NO.16156；Bacillus cereus, bacterial strain code are YJY18-09；Its deposit number
For CGMCC NO.16157；Nitrobacter winogradskyi, bacterial strain code are Y3-2, and deposit number is CCTCC NO:M2014203.
The bacterium amount of the bacterium powder is single bacterium 5 × 107~5 × 1010。
The microbial inoculum is carried out respectively by Candida tropicalis powder, Nitrobacter winogradskyi bacterium powder and bacillus cereus bacterium powder
After activation, composition is compounded according to the volume ratio of 1:1:1.
Preparation method, including following operating procedure:
(1) fermented and cultured: after candida tropicalis, Nitrobacter winogradskyi and bacillus cereus are inoculated in sterilizing respectively
Fermented and cultured obtains respective bacterium solution in fermentation medium；Under conditions of temperature is 20-40 DEG C, in fermentation tank culture to dissolved oxygen
It rises, pH decline；
(2) it prepares bacterium powder: after fermentation liquid is centrifuged, adding auxiliary materials and mixing, solid bacterium powder is obtained after drying；
(3) it mixes: above-mentioned bacterium powder is mixed according to mass ratio to get efficient acid water special bacteria agent is arrived.
More specifically steps are as follows:
1) actication of culture: on aseptic operating platform, the Candida tropicalis powder, the Nitrobacter winogradskyi that take 1~5 μ L to freeze
Bacterium powder and bacillus cereus are in the test tube containing LB liquid medium, and 28 DEG C~32 DEG C, 130~180rpm cultivates 18-
24h；
2) prepared by seed: on aseptic operating platform, the strain of activation being transferred to the triangle containing sterilized liquid LB culture medium
In bottle, packing 200mL culture medium in every triangular flask, test tube strains one triangular flask of inoculation of an activation, then 28 DEG C~
32 DEG C, 130~180rpm cultivates 18-24h；
3) inoculation fermentation tank: fermentation tank, pipeline, air filter are subjected to sky first and disappeared, 121 DEG C, sky disappears
30min injects culture medium after temperature reduction, then disappear in fact to culture medium, 121 DEG C, disappear 30min in fact；It is down to temperature
It is inoculated at 28 DEG C~32 DEG C；
4) fermentation condition: inoculum concentration v/v 5%~10%；28 DEG C~32 DEG C of cultivation temperature；PH:6.8-7.0；It is initial to turn
Speed: 200rpm；Fermentor pressure: 0.05Mpa；Ventilatory capacity ratio: 1:1；Fermentation period: 16~for 24 hours；
5) prepared by bacterium powder: after fermentation, addition diatomite, cornstarch adsorb thallus, dry later, crush and can obtain
To corresponding bacterium powder.
The culture medium for strain isolation is LB solid medium, culture medium prescription are as follows: peptone 10g/L,
NaCl10g/L, yeast extract 5g/L, pH 7.0-7.2, solvent is water.
The fermentation medium of the candida tropicalis forms are as follows: by weight are as follows: 1~2 part of peptone, 0.5-1 parts
Yeast extract, 1~3 part of sodium chloride, 94~97.5 parts of softened waters；
The fermentation medium of the bacillus cereus forms are as follows: by weight are as follows: 1-1.5 parts of glucose, 1-1.5 parts
Dregs of beans, 0.2-0.3 divide ammonium nitrate, 0.01-0.03 parts of manganese sulfates, 0.03-0.07 parts of magnesium sulfate, 0.02-0.05 parts of sodium chloride,
0.02-0.05 parts of calcium chloride, 0.003-0.005 parts of ferrous sulfate, 96.5-97.7 parts of softened waters；
The fermentation medium of the Nitrobacter winogradskyi forms are as follows: by weight are as follows: 1-3 parts of ammonium sulfate, 1-1.5 parts sub-
Sodium nitrate, 0.2-0.3 divide sodium dihydrogen phosphate, 0.1-1.0 parts of dipotassium hydrogen phosphates, 0.03-0.07 parts of magnesium sulfate, 3.0-5.0 parts of carbon
Sour calcium, 90-95.5 parts of softened waters.
Embodiment 2
A kind of efficient acid water special bacteria agent, is made of, respectively candida tropicalis, bacterial strain code the bacterium powder of three plants of bacterium
For YJY18-08, deposit number is CGMCC NO.16156；Bacillus cereus, bacterial strain code are YJY18-09；Its deposit number
For CGMCC NO.16157；Nitrobacter winogradskyi, bacterial strain code are Y3-2, and deposit number is CCTCC NO:M2014203.
The bacterium amount of the bacterium powder is single bacterium 5 × 107~5 × 1010。
The microbial inoculum is carried out respectively by Candida tropicalis powder, Nitrobacter winogradskyi bacterium powder and bacillus cereus bacterium powder
After activation, composition is compounded according to the volume ratio of 3:1:3.
Preparation method is the same as embodiment 1.
Embodiment 3
A kind of efficient acid water special bacteria agent, is made of, respectively candida tropicalis, bacterial strain code the bacterium powder of three plants of bacterium
For YJY18-08, deposit number is CGMCC NO.16156；Bacillus cereus, bacterial strain code are YJY18-09；Its deposit number
For CGMCC NO.16157；Nitrobacter winogradskyi, bacterial strain code are Y3-2, and deposit number is CCTCC NO:M2014203.
The bacterium amount of the bacterium powder is single bacterium 5 × 107~5 × 1010。
The microbial inoculum is carried out respectively by Candida tropicalis powder, Nitrobacter winogradskyi bacterium powder and bacillus cereus bacterium powder
After activation, composition is compounded according to the volume ratio of 2:1:2.
Preparation method is the same as embodiment 1.
Test example 1
Certain petro-chemical corporation's refinery device generates acid water, and ammonia nitrogen value is in 200mg/L or so, through analyzing, has containing main matter:
Volatile phenol 300mg/L or so；Sulfide 200mg/L or so.This waste water is handled with biochemical method, by system pH sodium hydroxide tune
To 6.5, nutrient salt solution is added, mix with aerobic activated sludge, the efficient acid water special bacteria agent of the acquisition of embodiment 1 is added,
Aeration culture, when ammonia nitrogen concentration drops to 20mg/L in culture solution, when static 2.5h, makes sludge settling, discards supernatant,
It is re-introduced into the acid water, steps up inflow, aerobic system has been resistant to ammonia nitrogen 200mg/L, and stable water outlet is in 20mg/ within two days
L, ammonia nitrogen removal frank is up to 90% or more.
The additional amount of the nutrient salt solution is the 0.001% of acid water quality；It is dirty that activity is added in every liter of acid water
Mud 5g.The efficient acid water special bacteria agent additional amount is the 0.01% of acid water quality.
The nutrient salt solution is dipotassium hydrogen phosphate 0.05g/L, potassium dihydrogen phosphate 0.05g/L, magnesium sulfate 0.0125g/
L, ammonium chloride 0.125g/L, sodium bicarbonate 0.031g/L, trace element solution 10mL, water complement to 1L.The microelement
Solution composition is ferrous sulfate 0.0625g/L, manganese sulfate 0.0125g/L, zinc chloride 0.025g/L, calcium chloride 0.0125g/L, water
Complement to 1L.
The biochemical inflow and outflow water quality technology table of table 1
| Serial number | Project | Raw water quality | Effluent quality |
| 1 | COD | 5000mg/L | < 200mg/L |
| 2 | Volatile phenol | 300mg/L | < 10mg/L |
| 3 | NH3-N | 200mg/L | < 20mg/L |
| 4 | Sulfide | 200mg/L | < 10mg/L |
| 5 | pH | 4.5-5.5 | 6.0-7.5 |
Test example 2
Generation acid water is set in certain petro-chemical corporation's coking makeup, and ammonia nitrogen value is analyzed in 100mg/L or so, contains main matter
Have: volatile phenol 300mg/L or so；Sulfide 300mg/L or so.This waste water is handled with biochemical method, by system pH hydroxide
Sodium is adjusted to 6.5, adds nutrient salt solution, mixes with aerobic activated sludge, and the efficient acid water special bacterium that embodiment 2 obtains is added
Agent, aeration culture, when ammonia nitrogen concentration drops to 20mg/L in culture solution, when static 2.5h, makes sludge settling, and it is clear to discard upper layer
Night is re-introduced into the acid water, steps up inflow, and aerobic system has been resistant to ammonia nitrogen 100mg/L, and stable water outlet exists within two days
20mg/L, ammonia nitrogen removal frank is up to 90% or more.
The additional amount of the nutrient salt solution is the 0.003% of acid water quality；It is dirty that activity is added in every liter of acid water
Mud 10g.The efficient acid water special bacteria agent additional amount is the 0.02% of acid water quality.
The nutrient salt solution is dipotassium hydrogen phosphate 0.05g/L, potassium dihydrogen phosphate 0.05g/L, magnesium sulfate 0.0125g/
L, ammonium chloride 0.125g/L, sodium bicarbonate 0.031g/L, trace element solution 10mL, water complement to 1L.The microelement
Solution composition is ferrous sulfate 0.0625g/L, manganese sulfate 0.0125g/L, zinc chloride 0.025g/L, calcium chloride 0.0125g/L, water
Complement to 1L.
The biochemical inflow and outflow water quality technology table of table 2
| Serial number | Project | Raw water quality | Effluent quality |
| 1 | COD | 3000mg/L | < 200mg/L |
| 2 | Volatile phenol | 300mg/L | < 10mg/L |
| 3 | NH3-N | 100mg/L | < 20mg/L |
| 4 | Sulfide | 300mg/L | < 10mg/L |
| 5 | pH | 9.5-10.5 | 6.0-7.5 |
Test example 3
Certain petro-chemical corporation's refinery device generates acid water, and ammonia nitrogen value is in 200mg/L or so, through analyzing, has containing main matter:
Volatile phenol 300mg/L or so；Sulfide 200mg/L or so.This waste water is handled with biochemical method, by system pH sodium hydroxide tune
To 6.5, nutrient salt solution is added, mix with aerobic activated sludge, the efficient acid water special bacteria agent of the acquisition of embodiment 3 is added,
Aeration culture, when ammonia nitrogen concentration drops to 20mg/L in culture solution, when static 2.5h, makes sludge settling, discards supernatant,
It is re-introduced into the acid water, steps up inflow, aerobic system has been resistant to ammonia nitrogen 200mg/L, and stable water outlet is in 20mg/ within two days
L, ammonia nitrogen removal frank is up to 90% or more.
The additional amount of the nutrient salt solution is the 0.03% of acid water quality；Activated sludge is added in every liter of acid water
8g.The efficient acid water special bacteria agent additional amount is the 0.05% of acid water quality.
The nutrient salt solution is dipotassium hydrogen phosphate 0.05g/L, potassium dihydrogen phosphate 0.05g/L, magnesium sulfate 0.0125g/
L, ammonium chloride 0.125g/L, sodium bicarbonate 0.031g/L, trace element solution 10mL, water complement to 1L.The microelement
Solution composition is ferrous sulfate 0.0625g/L, manganese sulfate 0.0125g/L, zinc chloride 0.025g/L, calcium chloride 0.0125g/L, water
Complement to 1L.
The biochemical inflow and outflow water quality technology table of table 3
| Serial number | Project | Raw water quality | Effluent quality |
| 1 | COD | 5000mg/L | < 200mg/L |
| 2 | Volatile phenol | 300mg/L | < 10mg/L |
| 3 | NH3-N | 200mg/L | < 20mg/L |
| 4 | Sulfide | 200mg/L | < 10mg/L |
| 5 | pH | 4.5-5.5 | 6.0-7.5 |
Claims (10)
1. a kind of efficient acid water special bacteria agent, it is characterised in that: be made of the bacterium powder of three plants of bacterium, respectively the false silk ferment in the torrid zone
Mother, bacterial strain code are YJY18-08, and deposit number is CGMCC NO.16156；Bacillus cereus, bacterial strain code are YJY18-
09；Its deposit number is CGMCC NO.16157；Nitrobacter winogradskyi, bacterial strain code are Y3-2, and deposit number is CCTCC NO:
M2014203。
2. efficient acid water special bacteria agent according to claim 1, it is characterised in that: the bacterium amount of the bacterium powder is single bacterium 5
×107~5 × 1010。
3. efficient acid water special bacteria agent according to claim 1, it is characterised in that: the microbial inoculum is by candida tropicalis
After bacterium powder, Nitrobacter winogradskyi bacterium powder and bacillus cereus bacterium powder are activated respectively, according to the volume ratio of 1~3:1:1~3
Compounding composition.
4. a kind of preparation method of efficient acid water special bacteria agent, which is characterized in that including following operating procedure:
(1) candida tropicalis, Nitrobacter winogradskyi and bacillus cereus fermented and cultured: are inoculated in sterilizing post-fermentation respectively
Fermented and cultured obtains respective bacterium solution in culture medium；Under conditions of temperature is 20-40 DEG C, fermentation tank culture to dissolved oxygen rises,
PH decline；
(2) it prepares bacterium powder: after fermentation liquid is centrifuged, adding auxiliary materials and mixing, solid bacterium powder is obtained after drying；
(3) it mixes: above-mentioned bacterium powder is mixed according to mass ratio to get efficient acid water special bacteria agent is arrived.
5. the preparation method of efficient acid water special bacteria agent according to claim 4, which is characterized in that more specifically walk
It is rapid as follows:
1) actication of culture: on aseptic operating platform, the Candida tropicalis powder, the Nitrobacter winogradskyi bacterium powder that take 1~5 μ L to freeze
With bacillus cereus in the test tube containing LB liquid medium, 28 DEG C~32 DEG C, 130~180rpm cultivates 18-24h；
2) prepared by seed: on aseptic operating platform, the strain of activation is transferred in the triangular flask containing sterilized liquid LB culture medium,
Packing 200mL culture medium in every triangular flask, test tube strains one triangular flask of inoculation of an activation, then 28 DEG C~32 DEG C,
130~180rpm cultivates 18-24h；
3) inoculation fermentation tank: carrying out fermentation tank, pipeline, air filter sky and disappear first, and 121 DEG C, sky disappears 30min, to
After temperature reduces, culture medium is injected, then culture medium disappear in fact, 121 DEG C, disappear 30min in fact；28 DEG C~32 are down to temperature
DEG C when be inoculated with；
4) fermentation condition: inoculum concentration v/v 5%~10%；28 DEG C~32 DEG C of cultivation temperature；PH:6.8-7.0；Initial speed:
200rpm；Fermentor pressure: 0.05Mpa；Ventilatory capacity ratio: 1:1；Fermentation period: 16~for 24 hours；
5) prepared by bacterium powder: after fermentation, addition diatomite, cornstarch adsorb thallus, dry later, phase can be obtained in crushing
Answer bacterium powder.
6. the preparation method of efficient acid water special bacteria agent according to claim 5, it is characterised in that: described is used for bacterial strain
Isolated culture medium is LB solid medium, culture medium prescription are as follows: peptone 10g/L, NaCl 10g/L, yeast extract 5g/L,
PH 7.0-7.2, solvent are water.
7. the preparation method of efficient acid water special bacteria agent according to claim 5, which is characterized in that the false silk in the torrid zone
The fermentation medium of yeast forms are as follows: by weight are as follows: 1~2 part of peptone, 0.5-1 parts of yeast extracts, 1~3 part of chlorination
Sodium, 94~97.5 parts of softened waters；
The fermentation medium of the bacillus cereus forms are as follows: by weight are as follows: 1-1.5 parts of glucose, 1-1.5 parts of beans
The dregs of rice, 0.2-0.3 divide ammonium nitrate, 0.01-0.03 parts of manganese sulfates, 0.03-0.07 parts of magnesium sulfate, 0.02-0.05 parts of sodium chloride,
0.02-0.05 parts of calcium chloride, 0.003-0.005 parts of ferrous sulfate, 96.5-97.7 parts of softened waters；
The fermentation medium of the Nitrobacter winogradskyi forms are as follows: by weight are as follows: 1-3 parts of ammonium sulfate, 1-1.5 parts of nitrous acid
Sodium, 0.2-0.3 divide sodium dihydrogen phosphate, 0.1-1.0 parts of dipotassium hydrogen phosphates, 0.03-0.07 parts of magnesium sulfate, 3.0-5.0 parts of calcium carbonate,
90-95.5 part softened water.
8. the application method of efficient acid water special bacteria agent described in claim 1, it is characterised in that: its detailed process are as follows:
Nutrient salt solution is added in oxytropism water, is mixed with activated sludge, system pH is adjusted to 6.0-7.0, is added efficiently acid
Water special bacteria agent, aeration culture 24-30h；The additional amount of the nutrient salt solution is the 0.001-0.05% of acid water quality；
Activated sludge 5-10g is added in every liter of acid water.
9. the application method of efficient acid water special bacteria agent according to claim 8, it is characterised in that: the nutritive salt
Solution is dipotassium hydrogen phosphate 0.05-0.4g/L, potassium dihydrogen phosphate 0.05-0.4g/L, magnesium sulfate 0.0125-0.1g/L, ammonium chloride
0.125-1.0g/L, sodium bicarbonate 0.031-0.25g/L, trace element solution 10-20mL, water complement to 1L.
10. the application method of efficient acid water special bacteria agent according to claim 9, it is characterised in that: described is micro
Element Solution group becomes ferrous sulfate 0.0625-0.5g/L, manganese sulfate 0.0125-0.1g/L, zinc chloride 0.025-0.2g/L, chlorine
Change calcium 0.0125-0.1g/L, water complements to 1L.
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| CN201811607374.4A CN109868233B (en) | 2018-12-27 | 2018-12-27 | Efficient special microbial inoculum for acidic water and application method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536417A (en) * | 2018-12-27 | 2019-03-29 | 黄河三角洲京博化工研究院有限公司 | A kind of biology drop phenol microbial inoculum and its application method |
| CN109554315A (en) * | 2018-12-27 | 2019-04-02 | 黄河三角洲京博化工研究院有限公司 | A kind of black and odorous water microorganism remediation composite bacteria agent and its application |
| CN111100824A (en) * | 2020-01-21 | 2020-05-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
| CN111117938A (en) * | 2020-02-21 | 2020-05-08 | 山东海景天环保科技股份公司 | Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694443A (en) * | 2015-03-30 | 2015-06-10 | 朱凌玮 | Improved biological microbial agent for disposing industrial sewage and preparation method and application thereof |
| CN105154366A (en) * | 2015-09-25 | 2015-12-16 | 哈尔滨理工大学 | Bacillus cereus capable of degrading phenol and having electrogenesis characteristic and application thereof |
| CN105198094A (en) * | 2015-10-22 | 2015-12-30 | 黄河三角洲京博化工研究院有限公司 | Method for performing intensified treatment on industrial high phenol-content wastewater |
| CN105274029A (en) * | 2015-11-11 | 2016-01-27 | 武汉水之国环保科技有限公司 | Nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, production method and application |
| CN105368745A (en) * | 2015-12-01 | 2016-03-02 | 深圳市大治生光环保科技有限公司 | Composite microbial preparation for treating black and odorous river and preparation method thereof |
| CN105441345A (en) * | 2015-12-21 | 2016-03-30 | 宁波大学 | Candida tropicalis and application thereof to biological desulphurization |
| CN106047772A (en) * | 2016-08-09 | 2016-10-26 | 湖北凌卓生物工程有限公司 | Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof |
-
2018
- 2018-12-27 CN CN201811607374.4A patent/CN109868233B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694443A (en) * | 2015-03-30 | 2015-06-10 | 朱凌玮 | Improved biological microbial agent for disposing industrial sewage and preparation method and application thereof |
| CN105154366A (en) * | 2015-09-25 | 2015-12-16 | 哈尔滨理工大学 | Bacillus cereus capable of degrading phenol and having electrogenesis characteristic and application thereof |
| CN105198094A (en) * | 2015-10-22 | 2015-12-30 | 黄河三角洲京博化工研究院有限公司 | Method for performing intensified treatment on industrial high phenol-content wastewater |
| CN105274029A (en) * | 2015-11-11 | 2016-01-27 | 武汉水之国环保科技有限公司 | Nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, production method and application |
| CN105368745A (en) * | 2015-12-01 | 2016-03-02 | 深圳市大治生光环保科技有限公司 | Composite microbial preparation for treating black and odorous river and preparation method thereof |
| CN105441345A (en) * | 2015-12-21 | 2016-03-30 | 宁波大学 | Candida tropicalis and application thereof to biological desulphurization |
| CN106047772A (en) * | 2016-08-09 | 2016-10-26 | 湖北凌卓生物工程有限公司 | Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| LUIS SAYAVEDRA-SOTO等: "Nitrobacter winogradskyi transcriptomic response to low and high ammonium concentrations", 《FEMS MICROBIOL LETT》 * |
| 徐振辞主编: "《水利科技发展与实践论文集》", 31 July 2008 * |
| 马杰: "炼化含酚废水降解菌剂的筛选复配研究与应用", 《中国优秀硕士学位论文全文数据库》 * |
| 黄海洪等: "一株芽孢杆菌在低氮源浓度培养基中的生长与氨氮去除特性", 《环境科学学报》 * |
| 黄炎坤等主编: "《现代养殖场生产设施与设备》", 30 September 2016 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536417A (en) * | 2018-12-27 | 2019-03-29 | 黄河三角洲京博化工研究院有限公司 | A kind of biology drop phenol microbial inoculum and its application method |
| CN109554315A (en) * | 2018-12-27 | 2019-04-02 | 黄河三角洲京博化工研究院有限公司 | A kind of black and odorous water microorganism remediation composite bacteria agent and its application |
| CN111100824A (en) * | 2020-01-21 | 2020-05-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
| CN111100824B (en) * | 2020-01-21 | 2021-11-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
| CN111117938A (en) * | 2020-02-21 | 2020-05-08 | 山东海景天环保科技股份公司 | Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater |
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