CN109868233B - Efficient special microbial inoculum for acidic water and application method thereof - Google Patents
Efficient special microbial inoculum for acidic water and application method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of sewage treatment, in particular to a high-efficiency special microbial inoculum for acidic water and an application method thereof. The high-efficiency acidic water special microbial inoculum consists of three strains of bacteria powder, namely candida tropicalis, wherein the strain code is YJY18-08, and the preservation number is CGMCC NO.16156; the bacillus cereus has a strain code of YJY18-09; the preservation number is CGMCC NO.16157; the nitrobacter winogradskyi has a strain code of Y3-2, and the preservation number of CCTCC NO: m2014203. The special high-efficiency acidic water treatment microbial inoculum is added into activated sludge, can effectively resist high load or inhibit the impact of harmful substances, improves the removal efficiency of volatile phenol, sulfide and ammonia nitrogen in a primary system, can save a physicochemical pretreatment process, saves cost, ensures that the treated sewage reaches the discharge standard of a comprehensive pool, and has no side effect on operators.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a high-efficiency special microbial inoculum for acidic water and an application method thereof.
Background
With the rapid development of the industries such as petrifaction, plastics, coking and the like, the generation of various acidic waters is increased correspondingly. In the processing processes of atmospheric and vacuum, catalytic cracking, thermal cracking, delayed coking, reforming and pre-hydrogenation, hydrofining, hydrocracking and the like, the compounds can generate H through reactions such as pyrolysis, catalytic cracking, catalytic hydrogenation and the like 2 S and NH 3 And in the product material flow, the product material flow is subjected to condensation dehydration or water washing treatment to generate sulfur-containing and ammonia-containing wastewater, commonly called sulfur-containing wastewater or acidic water, a specific drainage device comprises an atmospheric and vacuum distillation tower top separator, a catalytic cracking fractionating tower top separator, a catalytic cracking compressed rich gas dehydration tank, a coking fractionating tower top separator, high and low pressure separators for oil product hydrocracking and hydrofining and the like, and pollutants in the acidic water comprise oil, phenol, cyanogen and the like besides hydrogen sulfide and ammonia nitrogen. The concentration of the sulfide and the ammonia in the acid water changes with the types of crude oil and different processing devices, and in general, the higher the sulfur and nitrogen content of the crude oil is, the higher the concentration of the sulfide and the ammonia in the acid water is; the concentrations of the sulfide and the ammonia in the acid water are roughly divided into hydrocracking and hydrofining according to the processing device>Coking, catalytic cracking>Normal pressure and reduced pressure. Therefore, someone willThe acidic water discharged by the hydrocracking, hydrofining and reforming pre-hydrogenation devices is divided into high-concentration acidic water, the acidic water discharged by the catalytic cracking and coking devices is divided into medium-concentration acidic water, and the acidic water discharged by the atmospheric and vacuum devices is divided into low-concentration acidic water.
The small-water-volume and low-concentration acidic water treatment is developing to personalized technologies, such as a high-efficiency microbial technology, a chemical oxidative desulfurization-stripping deamination technology, a chemical oxidative desulfurization-biological denitrification technology and the like, in order to solve the problems of high operation load and high cost of a petrochemical stripping device, the project obtains a microbial inoculum for efficiently treating the acidic water by screening the microbial inoculum for reducing phenol, ammonia nitrogen, sulfide and COD, and constructs a biological flora, so that pollutants in the wastewater are converted into metabolic end products CO through the metabolic activity of microorganisms 2 And water and other harmless products, so that the waste water is purified. Meanwhile, the method has the remarkable advantages of low energy consumption, low running cost, simple process, no secondary pollution and the like.
Disclosure of Invention
The invention aims to provide a special high-efficiency microbial inoculum for acidic water and an application method thereof aiming at the problems. The microbial inoculum is developed according to the relatively low indexes of volatile phenol, sulfide and ammonia nitrogen in low-concentration acidic water. The special high-efficiency acidic water treatment microbial inoculum is added into a biochemical system, so that the removal efficiency of volatile phenol, ammonia nitrogen and sulfide in a primary system is improved, the load is effectively endured or the impact of substances is inhibited, and each index of effluent is ensured to reach the discharge standard.
The special high-efficiency acidic water microbial inoculum consists of three strains of bacteria powder, namely candida tropicalis, wherein the strain code is YJY18-08, and the preservation number is CGMCC NO.16156; the bacillus cereus has a strain code of YJY18-09; the preservation number is CGMCC NO.16157; the nitrobacter winogradskyi has a strain code of Y3-2, and the preservation number of CCTCC NO: m2014203.
The bacterial amount of the bacterial powder is 5 multiplied by 10 for a single bacterium 7 ～5×10 10 。
After the microbial inoculum is respectively activated by candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus powder, the ratio of the activated candida tropicalis powder to the activated nitrobacter winogradskyi powder to the activated bacillus cereus powder is 1-3: 1: 1-3, and the volume ratio is compounded.
According to the invention, the bacillus cereus is used for degrading volatile phenol, the nitrobacter winogradskyi is used for degrading ammonia nitrogen, and the candida tropicalis is used for degrading sulfide.
The preparation method of the special high-efficiency acidic water microbial inoculum comprises the following operation steps:
(1) Fermentation culture: respectively inoculating candida tropicalis, nitrobacter winogradskyi and bacillus cereus into a sterilized fermentation culture medium for fermentation culture to obtain respective bacterial liquids; culturing in a fermentation tank at 20-40 deg.C until dissolved oxygen rises and pH drops;
(2) Preparing bacterial powder: centrifuging the fermentation liquor, adding auxiliary materials, mixing uniformly, and drying to obtain solid fungus powder;
(3) Mixing: and mixing the bacterial powder according to a mass ratio to obtain the special bacterial agent for the high-efficiency acidic water.
The method comprises the following specific steps:
1) Activating strains: 1-5 mu L of frozen candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus are taken to be cultured in a test tube containing an LB liquid culture medium for 18-24h at 28-32 ℃ and 130-180 rpm on an aseptic operating platform;
2) Seed preparation: transferring the activated strain into a triangular flask containing a sterilized liquid LB culture medium on a sterile operating platform, subpackaging 200mL of the culture medium in each triangular flask, inoculating one activated test tube strain into one triangular flask, and then culturing at 28-32 ℃ and 130-180 rpm for 18-24h;
3) Inoculating a fermentation tank: firstly, performing air elimination on a fermentation tank body, a pipeline and an air filter for 30min at 121 ℃, injecting a culture medium after the temperature is reduced, and then performing actual elimination on the culture medium for 30min at 121 ℃; inoculating when the temperature is reduced to 28-32 ℃;
4) Fermentation conditions are as follows: the inoculation amount v/v is 5% -10%; the culture temperature is 28-32 ℃; pH:6.8-7.0; initial rotating speed: 200rpm; and (3) fermentation tank pressure: 0.05Mpa; air flow ratio: 1:1; and (3) fermentation period: 16-24 h;
5) Preparing fungus powder: after fermentation, adding diatomite and corn starch to adsorb bacteria, and then drying and crushing to obtain corresponding bacteria powder.
The culture medium for strain separation is an LB solid culture medium, and the formula of the culture medium is as follows: 10g/L of peptone, 10g/L of NaCl, 5g/L of yeast extract powder, 7.0-7.2 of pH and water as a solvent.
The fermentation medium of the candida tropicalis comprises the following components: the weight portions are as follows: 1-2 parts of peptone, 0.5-1 part of yeast extract, 1-3 parts of sodium chloride and 94-97.5 parts of softened water;
the fermentation medium of the bacillus cereus comprises the following components: the weight portions are as follows: 1-1.5 parts of glucose, 1-1.5 parts of soybean meal, 0.2-0.3 part of ammonium nitrate, 0.01-0.03 part of manganese sulfate, 0.03-0.07 part of magnesium sulfate, 0.02-0.05 part of sodium chloride, 0.02-0.05 part of calcium chloride, 0.003-0.005 part of ferrous sulfate and 96.5-97.7 parts of softened water;
the fermentation medium of the nitrobacter winogradskyi comprises the following components: the weight portions are: 1-3 parts of ammonium sulfate, 1-1.5 parts of sodium nitrite, 0.2-0.3 part of sodium dihydrogen phosphate, 0.1-1.0 part of dipotassium hydrogen phosphate, 0.03-0.07 part of magnesium sulfate, 3.0-5.0 parts of calcium carbonate and 90-95.5 parts of softened water.
The obtained high-efficiency acidic water special microbial inoculum is used for treating acidic water, and the specific process comprises the following steps:
adding nutrient salt into the acidic water, mixing with activated sludge, adjusting the pH of the system to 6.0-7.0 by using sulfuric acid, adding a high-efficiency acidic water special microbial inoculum, and carrying out aeration culture for 24-30h.
The nutrient salt solution is 0.05-0.4g/L of dipotassium phosphate, 0.05-0.4g/L of monopotassium phosphate, 0.0125-0.1g/L of magnesium sulfate, 0.125-1.0g/L of ammonium chloride, 0.031-0.25g/L of sodium bicarbonate, 10-20mL of trace element solution and the balance of water to 1L.
The microelement solution comprises ferrous sulfate 0.0625-0.5g/L, manganese sulfate 0.0125-0.1g/L, zinc chloride 0.025-0.2g/L, calcium chloride 0.0125-0.1g/L, and water in balance to 1L.
The addition amount of the nutrient salt solution is 0.001-0.05% of the mass of the acidic water; 5-10g of activated sludge is added into each liter of acid water.
The high-efficiency acidic water special-purpose microbial inoculum is applied to a treatment system, the phenol reducing capacity is more than 500 mg/(L.d), the desulfurization capacity is 300 mg/(L.d), the ammonia nitrogen reducing capacity reaches 50 mg/(L.d), and the high-efficiency acidic water special-purpose microbial inoculum is applied to an original treatment system, has strong adaptability to the system and has stable effect. Meanwhile, the high-efficiency strain has strong tolerance and wide application range.
The invention has the following advantages:
(1) By introducing exogenous microorganisms of candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus, the microbial activity and population advantages are enhanced, the removal efficiency of ammonia nitrogen and sulfide in a primary system is improved, the load or the impact of substances is effectively resisted, and the indexes of effluent are ensured to reach the discharge standard.
(2) The efficiency of desulfurization and ammonia nitrogen reduction is high, the sulfide of the effluent of each batch of aerobic system is less than 1mg/L, and the ammonia nitrogen is less than 5mg/L, thus reaching the standard of entering a comprehensive pool.
(3) The operation is simplified, the manpower and material resources are saved, and the use pressure of the equipment is reduced.
(4) Energy saving, environmental protection and no toxicity.
(5) The process flow is short, and the process operation safety is high.
Deposit description
Preservation time: 7 month and 25 days 2018
The name of the depository: china general microbiological culture Collection center
The preservation number is: CGMCC No.16156
The address of the depository: microbial research institute of Hospital, zhongkoyao No.1 Hotel, chaozhou, chaoyang, beijing
Classification nomenclature Candida tropicalis
Preservation time: 7 month and 25 days 2018
The name of the depository: china general microbiological culture Collection center
The preservation number is as follows: CGMCC No.16157
The address of the depository: microbial research institute of Hospital's institute of Zhongkou No.1 Xilu, beijing, chaoyang, and Hokkaido
Classification and nomenclature of Bacillus cereus
Detailed Description
The invention is further illustrated by the following examples.
Example 1
A high-efficiency acidic water special microbial inoculum consists of three strains of bacteria powder, namely candida tropicalis, wherein the strain code is YJY18-08, and the preservation number is CGMCC NO.16156; the bacillus cereus has a strain code of YJY18-09; the preservation number is CGMCC NO.16157; the nitrobacter winogradskyi has a strain code of Y3-2, and the preservation number of CCTCC NO: m2014203.
The bacterial amount of the bacterial powder is 5 multiplied by 10 for a single bacterium 7 ～5×10 10 。
After the microbial inoculum is respectively activated by candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus powder, the microbial inoculum is prepared by the following steps of 1:1:1, and the volume ratio is compounded.
The preparation method comprises the following operation steps:
(1) Fermentation culture: respectively inoculating candida tropicalis, nitrobacter winogradskyi and bacillus cereus into a sterilized fermentation culture medium for fermentation culture to obtain respective bacterial liquid; culturing in a fermentation tank at 20-40 deg.C until dissolved oxygen rises and pH drops;
(2) Preparing bacterial powder: centrifuging the fermentation liquor, adding auxiliary materials, mixing uniformly, and drying to obtain solid fungus powder;
(3) Mixing: and mixing the bacterial powder according to a mass ratio to obtain the special bacterial agent for the high-efficiency acidic water.
The method comprises the following specific steps:
1) Activating strains: 1-5 mu L of frozen candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus are taken to be placed in a test tube containing an LB liquid culture medium on a sterile operation table, and cultured for 18-24h at 28-32 ℃ and 130-180 rpm;
2) Seed preparation: transferring the activated strain into a triangular flask containing a sterile liquid LB culture medium on a sterile operating platform, subpackaging 200mL of the culture medium in each triangular flask, inoculating one activated test tube strain into one triangular flask, and then culturing at 28-32 ℃ and 130-180 rpm for 18-24h;
3) Inoculating a fermentation tank: firstly, performing air elimination on a fermentation tank body, a pipeline and an air filter for 30min at 121 ℃, injecting a culture medium after the temperature is reduced, and then performing actual elimination on the culture medium for 30min at 121 ℃; inoculating when the temperature is reduced to 28-32 ℃;
4) Fermentation conditions are as follows: the inoculation amount v/v is 5-10%; the culture temperature is 28-32 ℃; pH:6.8-7.0; initial rotating speed: 200rpm; and (3) fermentation tank pressure: 0.05Mpa; the air flow ratio is as follows: 1:1; and (3) fermentation period: 16-24 h;
5) Preparing fungus powder: after fermentation, adding diatomite and corn starch to adsorb bacteria, and then drying and crushing to obtain corresponding bacteria powder.
The culture medium for strain separation is an LB solid culture medium, and the formula of the culture medium is as follows: 10g/L of peptone, 10g/L of NaCl, 5g/L of yeast extract powder, 7.0-7.2 of pH and water as a solvent.
The fermentation medium of the candida tropicalis comprises the following components: the weight portions are as follows: 1-2 parts of peptone, 0.5-1 part of yeast extract, 1-3 parts of sodium chloride and 94-97.5 parts of softened water;
the fermentation medium of the bacillus cereus comprises the following components: the weight portions are as follows: 1-1.5 parts of glucose, 1-1.5 parts of soybean meal, 0.2-0.3 part of ammonium nitrate, 0.01-0.03 part of manganese sulfate, 0.03-0.07 part of magnesium sulfate, 0.02-0.05 part of sodium chloride, 0.02-0.05 part of calcium chloride, 0.003-0.005 part of ferrous sulfate and 96.5-97.7 parts of softened water;
the fermentation medium of the nitrobacter winogradskyi comprises the following components: the weight portions are as follows: 1-3 parts of ammonium sulfate, 1-1.5 parts of sodium nitrite, 0.2-0.3 part of sodium dihydrogen phosphate, 0.1-1.0 part of dipotassium hydrogen phosphate, 0.03-0.07 part of magnesium sulfate, 3.0-5.0 parts of calcium carbonate and 90-95.5 parts of softened water.
Example 2
A high-efficiency acidic water special microbial inoculum consists of three strains of bacteria powder, namely candida tropicalis, wherein the strain code is YJY18-08, and the preservation number is CGMCC NO.16156; the bacillus cereus has a strain code of YJY18-09; the preservation number is CGMCC NO.16157; the nitrobacter winogradskyi has a strain code of Y3-2, and the preservation number of CCTCC NO: m2014203.
The bacterial amount of the bacterial powder is 5 multiplied by 10 for a single bacterium 7 ～5×10 10 。
After the microbial inoculum is respectively activated by candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus powder, the microbial inoculum is prepared by the following steps: 1:3, and the components are compounded according to the volume ratio.
The preparation method is the same as that of example 1.
Example 3
A high-efficiency acidic water special microbial inoculum consists of three strains of bacteria powder, namely candida tropicalis, wherein the strain code is YJY18-08, and the preservation number is CGMCC NO.16156; the bacillus cereus has a strain code of YJY18-09; the preservation number is CGMCC NO.16157; the nitrobacter winogradskyi has a strain code of Y3-2, and the preservation number of CCTCC NO: m2014203.
The bacterial amount of the bacterial powder is 5 multiplied by 10 for a single bacterium 7 ～5×10 10 。
After the microbial inoculum is respectively activated by candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus powder, the microbial inoculum is prepared by the following steps of: 1:2, and the components are compounded according to the volume ratio.
The preparation method is the same as that of example 1.
Test example 1
A petrochemical device of a petrochemical company generates acid water, the ammonia nitrogen value is about 200mg/L, and the acid water contains the following main substances by analysis: volatile phenol about 300 mg/L; sulfide is about 200 mg/L. The wastewater is treated by a biochemical method, the pH of the system is adjusted to 6.5 by sodium hydroxide, a nutrient salt solution is added and mixed with aerobic activated sludge, the high-efficiency acidic water special-purpose microbial inoculum obtained in example 1 is added, aeration culture is carried out, when the concentration of ammonia nitrogen in a culture solution is reduced to 20mg/L, the sludge is settled after standing for 2.5h, the supernatant is discarded, the acidic water is introduced, the water inflow is gradually increased, the aerobic system can tolerate 200mg/L of ammonia nitrogen, the water outflow is stabilized at 20mg/L every two days, and the removal rate of the ammonia nitrogen can reach more than 90%.
The addition amount of the nutrient salt solution is 0.001 percent of the mass of the acidic water; 5g of activated sludge is added into each liter of acid water. The adding amount of the special high-efficiency acidic water microbial inoculum is 0.01 percent of the mass of the acidic water.
The nutrient salt solution is 0.05g/L of dipotassium hydrogen phosphate, 0.05g/L of monopotassium phosphate, 0.0125g/L of magnesium sulfate, 0.125g/L of ammonium chloride, 0.031g/L of sodium bicarbonate, 10mL of trace element solution and water which is complemented to 1L. The microelement solution comprises ferrous sulfate 0.0625g/L, manganese sulfate 0.0125g/L, zinc chloride 0.025g/L, calcium chloride 0.0125g/L, and water in balance to 1L.
TABLE 1 Biochemical water quality technical table for inlet water and outlet water
| Serial number | Item | Quality of raw water | Quality of effluent water |
| 1 | COD | 5000mg/L | ＜200mg/L |
| 2 | Volatile phenols | 300mg/L | ＜10mg/L |
| 3 | NH 3 -N | 200mg/L | ＜20mg/L |
| 4 | Sulfide compound | 200mg/L | ＜10mg/L |
| 5 | pH | 4.5-5.5 | 6.0-7.5 |
Test example 2
Acidic water is generated by a coking device of a petrochemical company, the ammonia nitrogen value is about 100mg/L, and the acidic water contains the following main substances by analysis: volatile phenol about 300 mg/L; about 300mg/L of sulfide. The wastewater is treated by a biochemical method, the pH of the system is adjusted to 6.5 by sodium hydroxide, a nutrient salt solution is added and mixed with aerobic activated sludge, the high-efficiency acidic water special-purpose microbial inoculum obtained in example 2 is added, aeration culture is carried out, when the concentration of ammonia nitrogen in a culture solution is reduced to 20mg/L, the sludge is settled after standing for 2.5 hours, the supernatant is discarded, the acidic water is introduced, the water inflow is gradually increased, the aerobic system can tolerate 100mg/L of ammonia nitrogen, the water outflow is stabilized at 20mg/L every two days, and the removal rate of the ammonia nitrogen can reach more than 90%.
The addition amount of the nutrient salt solution is 0.003 percent of the mass of the acidic water; 10g of activated sludge is added into each liter of acid water. The adding amount of the special high-efficiency acidic water microbial inoculum is 0.02 percent of the mass of the acidic water.
The nutrient salt solution is 0.05g/L of dipotassium hydrogen phosphate, 0.05g/L of monopotassium phosphate, 0.0125g/L of magnesium sulfate, 0.125g/L of ammonium chloride, 0.031g/L of sodium bicarbonate, 10mL of trace element solution and water which is complemented to 1L. The microelement solution comprises ferrous sulfate 0.0625g/L, manganese sulfate 0.0125g/L, zinc chloride 0.025g/L, calcium chloride 0.0125g/L, and water in balance to 1L.
TABLE 2 Biochemical water quality technical table for inlet water and outlet water
| Serial number | Item | Quality of raw water | Quality of effluent water |
| 1 | COD | 3000mg/L | ＜200mg/L |
| 2 | Volatile phenols | 300mg/L | ＜10mg/L |
| 3 | NH 3 -N | 100mg/L | ＜20mg/L |
| 4 | Sulfide compound | 300mg/L | ＜10mg/L |
| 5 | pH | 9.5-10.5 | 6.0-7.5 |
Test example 3
A petrochemical device of a petrochemical company generates acid water, the ammonia nitrogen value is about 200mg/L, and the acid water contains the following main substances by analysis: volatile phenol about 300 mg/L; sulfide is about 200 mg/L. The waste water is treated by a biochemical method, the pH value of the system is adjusted to 6.5 by sodium hydroxide, a nutrient salt solution is added and mixed with aerobic activated sludge, the high-efficiency acidic water special-purpose microbial inoculum obtained in the embodiment 3 is added, aeration culture is carried out, when the ammonia nitrogen concentration in a culture solution is reduced to 20mg/L, the sludge is settled after standing for 2.5 hours, the supernatant is discarded, the acidic water is introduced, the water inflow is gradually improved, the aerobic system can tolerate 200mg/L of ammonia nitrogen, the water outflow is stabilized at 20mg/L every two days, and the ammonia nitrogen removal rate can reach more than 90 percent.
The addition amount of the nutrient salt solution is 0.03 percent of the mass of the acidic water; 8g of activated sludge is added into each liter of acid water. The adding amount of the special high-efficiency acidic water microbial inoculum is 0.05 percent of the mass of the acidic water.
The nutrient salt solution is 0.05g/L of dipotassium hydrogen phosphate, 0.05g/L of monopotassium phosphate, 0.0125g/L of magnesium sulfate, 0.125g/L of ammonium chloride, 0.031g/L of sodium bicarbonate, 10mL of trace element solution and water which is complemented to 1L. The microelement solution comprises ferrous sulfate 0.0625g/L, manganese sulfate 0.0125g/L, zinc chloride 0.025g/L, calcium chloride 0.0125g/L, and water in balance to 1L.
TABLE 3 Biochemical water quality technical table
| Serial number | Item | Quality of raw water | Quality of effluent water |
| 1 | COD | 5000mg/L | ＜200mg/L |
| 2 | Volatile phenols | 300mg/L | ＜10mg/L |
| 3 | NH 3 -N | 200mg/L | ＜20mg/L |
| 4 | Sulfide compound | 200mg/L | ＜10mg/L |
| 5 | pH | 4.5-5.5 | 6.0-7.5 |
Claims (8)
1. A special microbial inoculum for high-efficiency acidic water is characterized in that: consists of three strains of bacteria powder, namely candida tropicalis respectively, wherein the strain code is YJY18-08, and the preservation number is CGMCC NO.16156; the bacillus cereus has a strain code of YJY18-09; the preservation number is CGMCC NO.16157; the nitrobacter winogradskyi has a strain code of Y3-2, and the preservation number of CCTCC NO: m2014203; after the microbial inoculum is respectively activated by candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus powder, the ratio of the activated candida tropicalis powder to the activated nitrobacter winogradskyi powder to the activated bacillus cereus powder is 1-3: 1: 1-3, and the volume ratio is compounded.
2. The preparation method of the high-efficiency acidic water special microbial inoculum is characterized by comprising the following operation steps of:
(1) Fermentation culture: respectively inoculating candida tropicalis, nitrobacter winogradskyi and bacillus cereus into a sterilized fermentation culture medium for fermentation culture to obtain respective bacterial liquids; culturing in a fermentation tank at 20-40 deg.C until dissolved oxygen rises and pH drops;
(2) Preparing bacterial powder: centrifuging the fermentation liquor, adding auxiliary materials, mixing uniformly, and drying to obtain solid fungus powder;
(3) Mixing: and mixing the bacterial powder according to a mass ratio to obtain the special bacterial agent for the high-efficiency acidic water.
3. The preparation method of the special high-efficiency acidic water microbial inoculum according to claim 2, which is characterized by comprising the following specific steps of:
1) Activating strains: 1-5 mu L of frozen candida tropicalis powder, nitrobacter winogradskyi powder and bacillus cereus are taken to be placed in a test tube containing an LB liquid culture medium on a sterile operation table, and cultured for 18-24h at 28-32 ℃ and 130-180 rpm;
2) Seed preparation: transferring the activated strain into a triangular flask containing a sterile liquid LB culture medium on a sterile operating platform, subpackaging 200mL of the culture medium in each triangular flask, inoculating one activated test tube strain into one triangular flask, and then culturing at 28-32 ℃ and 130-180 rpm for 18-24h;
3) Inoculating a fermentation tank: firstly, performing air elimination on a fermentation tank body, a pipeline and an air filter for 30min at 121 ℃, injecting a culture medium after the temperature is reduced, and then performing actual elimination on the culture medium for 30min at 121 ℃; inoculating when the temperature is reduced to 28-32 ℃;
4) Fermentation conditions are as follows: the inoculation amount v/v is 5% -10%; the culture temperature is 28-32 ℃; pH:6.8-7.0; initial rotating speed: 200rpm; and (3) fermentation tank pressure: 0.05Mpa; air flow ratio: 1:1; and (3) fermentation period: 16-24 h;
5) Preparing fungus powder: after fermentation, adding diatomite and corn starch to adsorb bacteria, and then drying and crushing to obtain corresponding bacteria powder.
4. The preparation method of the high-efficiency acidic water special microbial inoculum according to claim 3, which is characterized by comprising the following steps: the culture medium for strain separation is an LB solid culture medium, and the formula of the culture medium is as follows: 10g/L of peptone, 10g/L of NaCl, 5g/L of yeast extract powder, 7.0-7.2 of pH and water as a solvent.
5. The preparation method of the highly effective acidic water special bacterial agent as claimed in claim 3, wherein the fermentation medium of Candida tropicalis comprises: the weight portions are as follows: 1-2 parts of peptone, 0.5-1 part of yeast extract, 1-3 parts of sodium chloride and 94-97.5 parts of softened water;
the fermentation medium of the bacillus cereus comprises the following components: the weight portions are as follows: 1-1.5 parts of glucose, 1-1.5 parts of soybean meal, 0.2-0.3 part of ammonium nitrate, 0.01-0.03 part of manganese sulfate, 0.03-0.07 part of magnesium sulfate, 0.02-0.05 part of sodium chloride, 0.02-0.05 part of calcium chloride, 0.003-0.005 part of ferrous sulfate and 96.5-97.7 parts of softened water;
the fermentation medium of the nitrobacter winogradskyi comprises the following components: the weight portions are as follows: 1-3 parts of ammonium sulfate, 1-1.5 parts of sodium nitrite, 0.2-0.3 part of sodium dihydrogen phosphate, 0.1-1.0 part of dipotassium hydrogen phosphate, 0.03-0.07 part of magnesium sulfate, 3.0-5.0 parts of calcium carbonate and 90-95.5 parts of softened water.
6. The application method of the high-efficiency acidic water special-purpose microbial inoculum according to claim 1, is characterized in that: the specific process comprises the following steps:
adding nutrient salt solution into the acidic water, mixing with the activated sludge, adjusting the pH of the system to 6.0-7.0, adding the special high-efficiency acidic water microbial inoculum, and carrying out aeration culture for 24-30h; the addition amount of the nutrient salt solution is 0.001-0.05% of the mass of the acidic water;
5-10g of activated sludge is added into each liter of acid water.
7. The application method of the high-efficiency acidic water special microbial inoculum according to claim 6, which is characterized in that: the nutrient salt solution is 0.05-0.4g/L of dipotassium phosphate, 0.05-0.4g/L of monopotassium phosphate, 0.0125-0.1g/L of magnesium sulfate, 0.125-1.0g/L of ammonium chloride, 0.031-0.25g/L of sodium bicarbonate, 10-20mL of trace element solution and the balance of water to 1L.
8. The application method of the high-efficiency acidic water special microbial inoculum according to claim 7, which is characterized in that: the microelement solution comprises ferrous sulfate 0.0625-0.5g/L, manganese sulfate 0.0125-0.1g/L, zinc chloride 0.025-0.2g/L, calcium chloride 0.0125-0.1g/L, and water in balance to 1L.
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| CN109554315A (en) * | 2018-12-27 | 2019-04-02 | 黄河三角洲京博化工研究院有限公司 | A kind of black and odorous water microorganism remediation composite bacteria agent and its application |
| CN111100824B (en) * | 2020-01-21 | 2021-11-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
| CN111117938A (en) * | 2020-02-21 | 2020-05-08 | 山东海景天环保科技股份公司 | Compound microbial agent, preparation method thereof and treatment method of high-salt nitrogen-containing wastewater |
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