CN104891677B - A kind of preparation technology of the composite bacteria agent capable repairing Amino Acid Fermentation Wastewater - Google Patents
A kind of preparation technology of the composite bacteria agent capable repairing Amino Acid Fermentation Wastewater Download PDFInfo
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- CN104891677B CN104891677B CN201510360916.2A CN201510360916A CN104891677B CN 104891677 B CN104891677 B CN 104891677B CN 201510360916 A CN201510360916 A CN 201510360916A CN 104891677 B CN104891677 B CN 104891677B
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Abstract
The present invention relates to microbial technology field, being specifically related to the preparation technology of a kind of composite bacteria agent capable repairing Amino Acid Fermentation Wastewater, it comprises the steps: that step 1) prepares carrier, step 2) prepare composite bacteria liquid, the compound algae solution of step 3) preparation, step 4) prepares composite bacteria agent capable.Preparation technology simple possible of the present invention, is easily mastered, and is suitable for industrialization promotion and uses.
Description
Technical field
The present invention relates to microbial technology field, be specifically related to the preparation technology of a kind of composite bacteria agent capable repairing Amino Acid Fermentation Wastewater.
Background technology
In the industrial production, it is the organic wastewater of a kind of very high concentrations containing a large amount of amino acid whose waste water, there is organic concentration height, ammonia nitrogen concentration height, SO4 2-Or Cl-The features such as concentration is high, pH is low, are difficult to process.After the process such as this waste water separates through thalline, production yeast, anaerobic fermentation, aerobic aeration, although the COD of more than 90% can be removedCr, but the COD of waste waterCr, ammonia-nitrogen content still do not reach the sewage drainage standard of national regulation.These organic wastewaters quite a few directly drain into rivers, not only serious environment pollution, also valuable resource is thrown away as garbage；Meanwhile, high-concentration waste water causes Dissolved Oxygen in Water to reduce after entering water body, causes water quality deterioration, affects water ecological environment.
Processing waste water and major problem is that one-time investment is excessive, or day-to-day operation expense is too high, most producers cannot bear, it has to the present situation of long term maintenance discharge beyond standards.The previous research of applicant is to be used as to prepare fertilizer by waste water, its enterprise being suitable for possessing fertilizer production qualification and ability, but for other amino acids production enterprises, can not reach to produce the purpose of fertilizer.At present, studying hotter is that biological preparation processes waste water, and it possesses with low cost, does not results in the advantages such as secondary pollution, but it is unreasonable to possess microorganism compatibility, treatment effeciency is low, and microbe carrier to there is internal mass transfer poor, it is difficult to printing opacity, the defects such as specific surface area is little, and service life is short；Biological preparation needs to throw in often, adds cost, and injected volume is big, and the sludge quantity of generation is big, it is not easy to process.
Summary of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide the preparation technology of a kind of composite bacteria agent capable repairing Amino Acid Fermentation Wastewater.The present invention adopts the following technical scheme that realization:
A kind of preparation technology of the composite bacteria agent capable repairing Amino Acid Fermentation Wastewater, it comprises the steps: that step 1) prepares carrier, step 2) prepare composite bacteria liquid, the compound algae solution of step 3) preparation, step 4) prepares composite bacteria agent capable；
Specifically, comprise the steps:
Step 1) prepares carrier:
A): weigh following each raw material according to weight portion: water 20-22 part, humic acids 7-9 part, sucrose 6-8 part, Kaolin 3-4 part, ammonium sulfate 3-4 part, chitin 2-3 part, Cotton Gossypii 2-3 part, silica white 1-2 part；Preferably, the particle diameter of silica white is 200 mesh；
B): humic acids is pulverized, then adds Kaolin, mix homogeneously, be ground to the humic acids kaolin powder of 100 mesh；
C): by water, sucrose, ammonium sulfate, chitin, Cotton Gossypii and silica white add in stirred reactor successively, 200 turns/min stirs 10min, then adding humic acids kaolin powder, 500 turns/min stirs 3min, stands 12 hours subsequently, finally it is placed in 60 DEG C to dry to moisture less than 5%(parts by weight), obtain bio-carrier；
Step 2) prepare composite bacteria liquid:
Bacillus subtilis, Lactobacillus acidophilus, Acinetobacter bauamnnii and Sphingomonas are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:2:2:1, stands 6 hours；
The compound algae solution of step 3) preparation:
Scenedesmus obliquus and chlorella are cultivated respectively to concentration be 1 × 105The algae solution of individual/ml, then according to the volume ratio mixing of 1:1, stands 3 hours；
Step 4) prepares composite bacteria agent capable；
Composite bacteria liquid, compound algae solution and carrier are stood 24-36h according to the quality of 5:1:10 than mixing and stirring, room temperature, dry in the air to water content be 10%(parts by weight), to obtain final product.
Strain of the present invention and algae all can be commercially available from commercial sources such as preservation centers.
Strain of the present invention and algae all can obtain bacterium solution or the algae solution of desired concn, this and the innovative point of non-invention by conventional cultural method, as space is limited, repeat the most one by one.
The beneficial effect that the present invention obtains specifically includes that
Preparation technology simple possible of the present invention, those skilled in the art are easy to grasp, and are suitable for industrialization promotion and use；
The carrier that the present invention provides, can not only expand the specific surface area of carrier, and have that tensile strength is big, be evenly distributed, specific surface area big, service life the feature such as length；
The carrier that the present invention provides can be greatly improved the adhesion amount of microorganism, increases the biofilm biomass of entirety attachment, and microorganism concn is improved, and can reduce mud generation amount；
The existence of anaerobism, anoxia and aerobic various environment that the carrier that the present invention provides provides can promote the treatment effect of microorganism, simultaneously facilitates the removal of the pollutant such as ammonia nitrogen in the minimizing of mud, beneficially waste water；
The composite bacteria agent capable of the present invention, by the various strains that can form dominant microflora and algae, is configured to high-performance bio preparation, reasonable compatibility between each microorganism, and symbiosis is coordinated, the most not antagonism, and Biomass is big, and breeding is fast.
Composite bacteria agent capable of the present invention is suitable to Amino Acid Fermentation Wastewater and processes, it is simple to transports and preserves, and is easier to activate during use, and operating cost is cheap.
Detailed description of the invention
For the technical scheme making those skilled in the art be more fully understood that in the application, below in conjunction with the application specific embodiment, the technical scheme of the application is clearly and completely described, it is clear that, described embodiment is only some embodiments of the present application rather than whole embodiments.Based on the embodiment in the application, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all should belong to the scope of protection of the invention.
Embodiment 1
The preparation technology of a kind of composite bacteria agent capable repairing Amino Acid Fermentation Wastewater, it comprises the steps:
Step 1) prepares carrier:
A): weigh following each raw material according to weight portion: 20 parts of water, humic acids 7 parts, sucrose 6 parts, Kaolin 3 parts, 3 parts of ammonium sulfate, chitin 2 parts, 2 parts of Cotton Gossypii, silica white 1 part；
B): humic acids is pulverized, then adds Kaolin, mix homogeneously, be ground to the humic acids kaolin powder of 100 mesh；
C): by water, sucrose, ammonium sulfate, chitin, Cotton Gossypii and silica white add in stirred reactor successively, 200 turns/min stirs 10min, then adding humic acids kaolin powder, 500 turns/min stirs 3min, stands 12 hours subsequently, finally it is placed in 60 DEG C to dry to moisture less than 5%(parts by weight), obtain bio-carrier；
Step 2) prepare composite bacteria liquid:
Bacillus subtilis, Lactobacillus acidophilus, Acinetobacter bauamnnii and Sphingomonas are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:2:2:1, stands 6 hours；
The compound algae solution of step 3) preparation:
Scenedesmus obliquus and chlorella are cultivated respectively to concentration be 1 × 105The algae solution of individual/ml, then according to the volume ratio mixing of 1:1, stands 3 hours；
Step 4) prepares composite bacteria agent capable；
Composite bacteria liquid, compound algae solution and carrier are stood 24-36h according to the quality of 5:1:10 than mixing and stirring, room temperature, dry in the air to water content be 10%(parts by weight), to obtain final product.
Embodiment 2
The preparation technology of a kind of composite bacteria agent capable repairing Amino Acid Fermentation Wastewater, it comprises the steps:
Step 1) prepares carrier:
A): weigh following each raw material according to weight portion: 22 parts of water, humic acids 9 parts, sucrose 8 parts, Kaolin 4 parts, 4 parts of ammonium sulfate, chitin 3 parts, 3 parts of Cotton Gossypii, silica white 2 parts；Wherein, the particle diameter of silica white is 200 mesh；
B): humic acids is pulverized, then adds Kaolin, mix homogeneously, be ground to the humic acids kaolin powder of 100 mesh；
C): by water, sucrose, ammonium sulfate, chitin, Cotton Gossypii and silica white add in stirred reactor successively, 200 turns/min stirs 10min, then adding humic acids kaolin powder, 500 turns/min stirs 3min, stands 12 hours subsequently, finally it is placed in 60 DEG C to dry to moisture less than 5%(parts by weight), obtain bio-carrier；
Step 2) prepare composite bacteria liquid:
Bacillus subtilis, Lactobacillus acidophilus, Acinetobacter bauamnnii and Sphingomonas are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:2:2:1, stands 6 hours；
The compound algae solution of step 3) preparation:
Scenedesmus obliquus and chlorella are cultivated respectively to concentration be 1 × 105The algae solution of individual/ml, then according to the volume ratio mixing of 1:1, stands 3 hours, to obtain final product；
Step 4) prepares composite bacteria agent capable；
Composite bacteria liquid, compound algae solution and carrier are stood 24-36h according to the quality of 5:1:10 than mixing and stirring, room temperature, dry in the air to water content be 10%(parts by weight), to obtain final product.
Wherein, bacillus subtilis is (Bacillus subtilis) CGMCC NO:2947 (can be found in CN101838621A), and Lactobacillus acidophilus (can be found in Appl. for (Lactobacillus acidophilus) ATCC4356
Environ. Microbiol.December 2008 vol. 74, no. 24 7824-7827), Acinetobacter bauamnnii is that ATCC 19606(can be found in Infect Immun. 2012 Mar for (Acinetobacter baumanii);80 (3): 1015-24), Sphingomonas is (Sphingomonas
Sp.) CGMCC NO.4589(can be found in CN102168054A), scenedesmus obliquus can be found in CN103484374A for (Scenedesmus obliquus) CGMCC No.8015(), chlorella is (Chlorella sp.) CGMCC NO:3037(CN101565675A).
Embodiment 3
The water treatment effect test of the composite bacteria agent capable of the embodiment of the present invention 2 preparation:
Test with the fermentation waste water removing the materials such as fermentation thalli, polysaccharide and polysaccharide for processing sample.
Matched group 1: use sawdust carrier；Microorganism type is identical with test group；Processing mode: after adding three days continuously, every day, 5g/ cubic meter, then stood six days, and detection result is shown in Table 1.
Matched group 2: carrier is same as in Example 2, is a difference in that, only adds four kinds of bacterium, without compound algae solution；Processing mode: add 5g/ cubic meter for first day, then stand six days, detection result is shown in Table 1.
Test group: the composite bacteria agent capable of the embodiment of the present invention 2 preparation；Processing mode: after adding one day, 5g/ cubic meter, then stand six days, detection result is shown in Table 1.
Table 1
| Before process (mg/L) | After process (matched group 1) (mg/L) | After process (matched group 2) (mg/L) | (test group) (mg/L) after process | |
| COD | 1217 | 13.3 | 40.6 | 6.2 |
| NH3-N | 204 | 10.6 | 17.8 | 5.3 |
| Sulfide | 27 | 4.3 | 3.5 | 1.1 |
| Chloride ion | 6.2 | 1.3 | 1.9 | 0.3 |
Conclusion: carrier prepared by the present invention is big empty network structure, is twice above than the specific surface area of conventional sawdust carrier, and microorganism adhesion effect is good, and putting into number of times and weight all reduces, service life is long, it is not necessary to repeatedly throw in, save cost, reduce the generation amount of mud.Mushroom and algae reasonable compatibility in composite bacteria agent capable prepared by the present invention, it is possible to effectively remove the pollutant such as ammonia nitrogen, sulfide and chloride in fermentation waste water, be fully achieved discharge standard.
Although, the most illustrating by generality and detailed explanation has been made in this case by detailed description of the invention, but on the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, the amendment made without departing from theon the basis of the spirit of the present invention or improvement, belong to the scope of protection of present invention.
Claims (1)
1. the preparation technology of the composite bacteria agent capable repairing Amino Acid Fermentation Wastewater, it is characterised in that described technique comprises the steps:
Step 1) prepares carrier:
A): weigh following each raw material according to weight portion: water 20-22 part, humic acids 7-9 part, sucrose 6-8 part, Kaolin 3-4 part, ammonium sulfate 3-4 part, chitin 2-3 part, Cotton Gossypii 2-3 part, silica white 1-2 part；The particle diameter of described silica white is 200 mesh；
B): humic acids is pulverized, then adds Kaolin, mix homogeneously, be ground to the humic acids kaolin powder of 100 mesh；
C): by water, sucrose, ammonium sulfate, chitin, Cotton Gossypii and silica white add in stirred reactor successively, 200 turns/min stirs 10min, then adding humic acids kaolin powder, 500 turns/min stirs 3min, stands 12 hours subsequently, finally it is placed in 60 DEG C to dry to moisture less than 5%, obtains bio-carrier；
Step 2) prepare composite bacteria liquid:
Bacillus subtilis, Lactobacillus acidophilus, Acinetobacter bauamnnii and Sphingomonas are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:2:2:1, stands 6 hours；
The compound algae solution of step 3) preparation:
Scenedesmus obliquus and chlorella are cultivated respectively to concentration be 1 × 105The algae solution of individual/ml, then according to the volume ratio mixing of 1:1, stands 3 hours；
Step 4) prepares composite bacteria agent capable:
Than mixing and stirring, room temperature, composite bacteria liquid, compound algae solution and carrier are stood 24-36h according to the quality of 5:1:10, and drying in the air to water content is 10%, to obtain final product.
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| CN113402124B (en) * | 2021-07-02 | 2022-09-16 | 内蒙古阜丰生物科技有限公司 | Method for reducing blood sugar and collecting salt from amino acid wastewater |
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| CN104230004A (en) * | 2014-10-16 | 2014-12-24 | 内蒙古阜丰生物科技有限公司 | Biological agent for processing glutamic acid fermentation waste water |
| CN104261631A (en) * | 2014-10-14 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Environment-friendly process for treating glutamic acid fermentation wastewater |
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| CN104261631A (en) * | 2014-10-14 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Environment-friendly process for treating glutamic acid fermentation wastewater |
| CN104230004A (en) * | 2014-10-16 | 2014-12-24 | 内蒙古阜丰生物科技有限公司 | Biological agent for processing glutamic acid fermentation waste water |
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