CN104230004B - A kind of biotechnological formulation processing glutamic acid fermentation waste water - Google Patents

A kind of biotechnological formulation processing glutamic acid fermentation waste water Download PDF

Info

Publication number
CN104230004B
CN104230004B CN201410548050.3A CN201410548050A CN104230004B CN 104230004 B CN104230004 B CN 104230004B CN 201410548050 A CN201410548050 A CN 201410548050A CN 104230004 B CN104230004 B CN 104230004B
Authority
CN
China
Prior art keywords
parts
waste water
glutamic acid
liquid
acid fermentation
Prior art date
Application number
CN201410548050.3A
Other languages
Chinese (zh)
Other versions
CN104230004A (en
Inventor
王均成
张传森
包鑫
程文焕
卢松
朱心双
贺雪梅
Original Assignee
内蒙古阜丰生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 内蒙古阜丰生物科技有限公司 filed Critical 内蒙古阜丰生物科技有限公司
Priority to CN201410548050.3A priority Critical patent/CN104230004B/en
Publication of CN104230004A publication Critical patent/CN104230004A/en
Application granted granted Critical
Publication of CN104230004B publication Critical patent/CN104230004B/en

Links

Abstract

The present invention relates to a kind of biotechnological formulation for the treatment of glutamic acid fermentation waste water, tropina is separated through high-speed dish piece separating machine by its glutami acid fermentation liquor, collect bacteria-removing liquid, and precipitation tropina, tropina is added compound seed liquid fermentation for probiotics preparation, waste water bacteria-removing liquid being extracted L-glutamic acid generation enters and enters Sewage treatment systems, qualified discharge after interpolation complex micro organism fungicide advanced treatment, with the economic environmental protection of present invention process, it has broad application prospects.

Description

A kind of biotechnological formulation processing glutamic acid fermentation waste water

Technical field

The present invention relates to biological fermentation industry extraction technology of glutamic acid field, a kind of biotechnological formulation processing glutamic acid fermentation waste water is specifically provided.

Background technology

Along with the develop rapidly of economy and the continuous progress of technology, China has become the production and consumption big country of monosodium glutamate, but the wastewater flow rate discharged in glutamate production process is large, the mother liquor that fermented gournet powder liquid discharges after waiting electricity to extract L-glutamic acid has that CODCr is high, BOD5 is high, thalline content is high, content is high, ammonia-nitrogen content is high and the feature of pH value (1.5-3.2) low " five high low " for sulfate radical (use instead sulfuric acid adjust pH before be chlorion).It is the trade effluent that a kind of difficulty of governance is very large.Owing to effectively can not administer gourmet powder waste water, many Gourmet Powder Factories are put into the row of national major polluting sources unit, and the improvement of gourmet powder waste water has become the great difficult problem of restriction glutamate production enterprise development.

On the one hand: the extraction of monosodium glutamate such as to adopt usually at the electricity-from friendship method, iso-electric point is regulated to make glutamic acid crystallization out by adding the vitriol oil, and the ammonium sulphate waste liquor produced in production process, bring greatly difficulty to liquid waste disposal, direct harm is caused to environment, water source.

On the other hand: the greatest contamination source in monosodium glutamate industry Ye Shi China fermentation industry, according to statistics, msg product per ton produces high-concentration waste water about 15 tons.Monosodium glutamate industry high concentrated organic wastewater is seriously polluted, is the common problem that industry is outstanding.Fermentation waste liquor or glutamate wastewater are the primary pollution source of glutamate production industry.

The a large amount of thalline contained in extracting glutamic acid waste water, it is a kind of single cell protein, containing rich in protein, carry out analyzing to the chemical composition of tropina after drying and find that content that L-glutamic acid discards protein in thalline is 78.77% up to 85.8% total amino acid content, higher than raw material dregs of beans, yeast etc. that current protein zymolyte is conventional.Its amino acid classes and proportioning are all more complete, and containing abundant other nutritive substances such as VITAMIN, nucleic acid, polysaccharide.And add glucide in glutamic acid fermentation process can generate oligomeric isomaltose afterwards by fermentation together with L-glutamic acid.Glutamic acid fermentation generation high-concentration waste water carries out bipolar membrane electrodialysis and carries out desalting treatment after ultrafiltration membrance filter, and the waste water after desalination can be used for producing fertilizer, and the clear liquid after the desalination obtained contains a large amount of oligomeric isomaltoses.These useful matteies discharge in vain, cause a large amount of loss and wastes every year.

It is reported, often produce 1t monosodium glutamate, approximately will discharge the mother liquor after 10-15 ton extraction L-glutamic acid, the whole nation will discharge 1,000 ten thousand tons of this high concentrated organic wastewaters every year.Not only severe contamination physical environment, and constrain the development of monosodium glutamate industry.Although glutamate production enterprise, scientific research institution and relevant universities and colleges have all carried out large quantifier elimination to improvement.But, all also do not have ripe complete set technology to be applied to production practice at present both at home and abroad.Main problem is that one-time investment is excessive, or day-to-day operation expense is too high, and most Gourmet Powder Factory cannot bear, the present situation of long term maintenance of having to discharge beyond standards.

Therefore, studying a kind of environmental protection preparation processing glutamic acid fermentation waste water, to reduce contaminated wastewater, to turn waste into wealth, is the technical problem that this area needs solution badly.

Summary of the invention

The object of the invention is the deficiency for traditional technology, provide a kind of biotechnological formulation processing glutamic acid fermentation waste water, it significantly reduces production cost, and production process is easy and simple to handle, stable and reliable product quality.Meet the requirement of comprehensive utilization of resources, energy-saving and emission-reduction, decrease discharging of waste liquid simultaneously, alleviate sewage disposal burden, bring huge economic benefit and environmental benefit.In order to realize the object of the invention, adopt following technical scheme:

Process a biotechnological formulation for glutamic acid fermentation waste water, it is characterized in that described biotechnological formulation is that complex micro organism fungicide and carrier are mixed with according to the weight ratio of 1:1-2:1, the activeconstituents of described complex micro organism fungicide comprises the raw material of following weight part:

10 parts, yeast, subtilis 7 parts, bacillus megaterium 6 parts, denitrifying bacteria 5 parts, pseudomonas aeruginosa 5 parts, rhodococcus 4 parts, Phanerochaete chrysosporium 3 parts

Described carrier is chitosan.

Described yeast is yeast (Candida santamariae) CGMCC NO 2959;

Described subtilis is subtilis (Bacillus subtilis) CGMCC NO 2947

Described bacillus megaterium is bacillus megaterium (Bacillus megatherium) CGMCC No:1487;

Described denitrifying bacteria is denitrifying bacteria (Paracoccus pantotrophus) ATCC 35512;

Described pseudomonas aeruginosa is pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC15442;

Described rhodococcus be rhodococcus ( rhodococcus rhodochrous) ATCC 15906;

Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725.

The method of described biotechnological formulation clean glutamic acid fermentation process is utilized to be:

(1) tropina is separated through high-speed dish piece separating machine by glutami acid fermentation liquor, and the rotating speed of high-speed dish piece machine separating thallus is 4000 ~ 5000r/min, collects bacteria-removing liquid, and precipitation tropina;

(2) tropina that prepared by step (1) adds appropriate warm water and mixes well, adjustment solid content 15%, in input fermentation unit, pH is regulated to be 6.0-7.0 with milk of lime, access 1/10 (V/V, volume adds water to mix well 1/10 of the rear tropina aqueous solution) compound seed liquid, stir, control temperature 32-34 DEG C, the ventilation of culturing process discontinuous is also slowly stirred, cultivate 24h, obtain maturing fermentation liquid probiotic agent;

Described compound seed liquid is that yeast saccharomyces cerevisiae and plant lactobacillus obtain according to volume ratio 3:1 preparation, described yeast saccharomyces cerevisiae is preferably: CCTCC NO:M208110 (CN101434911), described plant lactobacillus is preferably: CCTCC M208151 (CN101748082), yeast saccharomyces cerevisiae and plant lactobacillus is conveniently cultivated concentration and all controls 1 × 10 8individual/ml, the bacterium liquid cultivated is mixed to get compound seed liquid according to volume ratio 3:1;

(3) bacteria-removing liquid that prepared by step (1) pumps into bleacher and carries out desolventing technology, the powdered carbon of bacteria-removing liquid quality 1.5% is added in bleacher, the temperature controlled in bleacher is 45-50 DEG C, decolour 30 points afterwards concentrated, described concentrated parameter be: temperature 60-70 DEG C, vacuum tightness is-0.1kpa, and primary crystallization obtains coarse crystal; By coarse crystal separation and purification, decolouring, ion-exchange, secondary crystal, is separated, dry, and screening is refining;

(4) get the factory effluent that above-mentioned steps (3) extraction step produces, natural subsidence solid-liquid separation, obtain sediment and supernatant liquor, supernatant liquor is entered and enters Sewage treatment systems, qualified discharge after interpolation complex micro organism fungicide advanced treatment.

The activeconstituents of described complex micro organism fungicide comprises the raw material of following weight part:

10 parts, yeast, subtilis 7 parts, bacillus megaterium 6 parts, denitrifying bacteria 5 parts, pseudomonas aeruginosa 5 parts, rhodococcus 4 parts, Phanerochaete chrysosporium 3 parts.

Described yeast is yeast (Candida santamariae) CGMCC NO 2959 (CN101629144A);

Described subtilis is subtilis (Bacillus subtilis) CGMCC NO 2947 (CN101838621A)

Described bacillus megaterium is bacillus megaterium (Bacillus megatherium) CGMCC No:1487 (CN101074421A);

Described denitrifying bacteria is denitrifying bacteria (Paracoccus pantotrophus) ATCC 35512;

Described pseudomonas aeruginosa is pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC15442;

Described rhodococcus be rhodococcus ( rhodococcus rhodochrous) ATCC 15906; (see document Cloning and Characterization of Benzoate Catabolic Genes in the Gram-Positive Polychlorinated Biphenyl DegraderRhodococcus sp. Strain RHA1, J. Bacteriol. November 2001);

Described Phanerochaete chrysosporium be Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725(see document APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714)

2011);

Above yeast, subtilis, bacillus megaterium, denitrifying bacteria, pseudomonas aeruginosa, rhodococcus, Phanerochaete chrysosporium are conveniently cultivated concentration all control 2 × 10 8individual/gram, the bacterium liquid cultivated is mixed to get liquid bacterial agent according to mass ratio;

Getting aforesaid liquid microbial inoculum and carrier is uniformly mixed, is preferably carrier with chitosan, according to microbial inoculum: carrier is 2:(1-2) weight ratio mixing.Dry: will mix material and carry out drying, drying temperature is 20-50 DEG C, after dry, water content is 20-30%; Inspection, packaging: by quality standard inspection, finished product is packed by weight, obtains solid fungicide.

Add microbial solid inocula 20-30 gram by every cubic metre of still bed material at every turn, add 1 every day, add one week continuously, finally leave standstill 3 days, liquid is discharged.

The beneficial effect that the present invention obtains:

1 with discarded tropina for raw material, fermentable prepares probiotic agent, turns waste into wealth, and solves problem of environmental pollution, and the microbial inoculum of preparation promotes growth of animal, improves animal disease resistant ability, also creates objective economic benefit.

2 composite fungus agents are specially for the waste water of extracting glutamic acid preparation process of the present invention, by the various bacterial classification that can form dominant microflora, be mixed with high-performance bio preparation, be added in Waste Water Treatment by a certain amount of, accelerate the degraded of microbe, to improve the biological treatment efficiency of system, ensure system stable operation.It contains multiple microorganism Recalcitrant chemicals being had to excellent degradation capability, reasonable compatibility between each bacterial classification, symbiosis is coordinated, mutual not antagonism, active high, biomass is large, breeding is fast, add in Waste Water Treatment, have good degradation effect to macromole, hard-degraded substance, have unique treatment effect to traditional propylhomoserin process discharge waste water.Be suitable for the application preparation method and produce discharge of wastewater process, the process water yield and water quality treatment can be improved, reduce working cost, promote qualified discharge.

embodiment:

Embodiment 1:

Get the glutami acid fermentation liquor in Fu Feng workshop, adopt following steps:

(1) tropina is separated through high-speed dish piece separating machine by glutami acid fermentation liquor, and the rotating speed of high-speed dish piece machine separating thallus is 4000 ~ 5000r/min, collects bacteria-removing liquid, and precipitation tropina;

(2) tropina that prepared by step (1) adds appropriate warm water and mixes well, adjustment solid content 15%, in input fermentation unit, pH is regulated to be 6.0-7.0 with milk of lime, access 1/10 (V/V, volume adds water to mix well 1/10 of the rear tropina aqueous solution) compound seed liquid, stir, control temperature 32-34 DEG C, the ventilation of culturing process discontinuous is also slowly stirred, cultivate 24h, obtain maturing fermentation liquid probiotic agent;

Described compound seed liquid is that yeast saccharomyces cerevisiae and plant lactobacillus obtain according to volume ratio 3:1 preparation, described yeast saccharomyces cerevisiae is: CCTCC NO:M208110 (CN101434911), described plant lactobacillus is: CCTCC M208151 (CN101748082), yeast saccharomyces cerevisiae and plant lactobacillus is conveniently cultivated concentration and all controls 1 × 10 8individual/ml, the bacterium liquid cultivated is mixed to get compound seed liquid according to volume ratio 3:1;

(3) bacteria-removing liquid that prepared by step (1) pumps into bleacher and carries out desolventing technology, the powdered carbon of bacteria-removing liquid quality 1.5% is added in bleacher, the temperature controlled in bleacher is 45-50 DEG C, decolour 30 points afterwards concentrated, described concentrated parameter be: temperature 60-70 DEG C, vacuum tightness is-0.1kpa, and primary crystallization obtains coarse crystal; By coarse crystal separation and purification, decolouring, ion-exchange, secondary crystal, is separated, dry, and screening is refining;

(4) get the factory effluent that above-mentioned steps (3) extraction step produces, natural subsidence solid-liquid separation, obtain sediment and supernatant liquor, supernatant liquor is entered and enters Sewage treatment systems, qualified discharge after interpolation complex micro organism fungicide advanced treatment.

The activeconstituents of described complex micro organism fungicide comprises the raw material of following weight part:

10 parts, yeast, subtilis 7 parts, bacillus megaterium 6 parts, denitrifying bacteria 5 parts, pseudomonas aeruginosa 5 parts, rhodococcus 4 parts, Phanerochaete chrysosporium 3 parts.

Described yeast is yeast (Candida santamariae) CGMCC NO 2959 (CN101629144A);

Described subtilis is subtilis (Bacillus subtilis) CGMCC NO 2947 (CN101838621A)

Described bacillus megaterium is bacillus megaterium (Bacillus megatherium) CGMCC No:1487 (CN101074421A);

Described denitrifying bacteria is denitrifying bacteria (Paracoccus pantotrophus) ATCC 35512;

Described pseudomonas aeruginosa is pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC15442;

Described rhodococcus be rhodococcus ( rhodococcus rhodochrous) ATCC 15906; (see document Cloning and Characterization of Benzoate Catabolic Genes in the Gram-Positive Polychlorinated Biphenyl DegraderRhodococcus sp. Strain RHA1, J. Bacteriol. november 2001);

Described Phanerochaete chrysosporium be Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725(see document APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714)

2011);

Above yeast, subtilis, bacillus megaterium, denitrifying bacteria, pseudomonas aeruginosa, rhodococcus, Phanerochaete chrysosporium are conveniently cultivated concentration all control 2 × 10 8individual/gram, the bacterium liquid cultivated is mixed to get liquid bacterial agent according to mass ratio;

Getting aforesaid liquid microbial inoculum and carrier is uniformly mixed, is carrier with chitosan, according to microbial inoculum: carrier is the weight ratio mixing of 1:1.Dry: will mix material and carry out drying, drying temperature is 20-50 DEG C, after dry, water content is 20-30%; Inspection, packaging: by quality standard inspection, finished product is packed by weight, obtains solid fungicide.

Add microbial solid inocula 20 grams by every cubic metre of still bed material at every turn, add 1 every day, add one week continuously, finally leave standstill 3 days, liquid is discharged.

Embodiment 2: probiotic agent effect test prepared by embodiment 1:

Test is Duroc with pig

Control group drinking public water supply; Test group drinks the 1% probiotic agent aqueous solution (weight ratio), average starting weight 24.2 kg,

Test group and control group with pig each 20, random packet, similarity condition raises 98 d, and growing state is as shown in table 1:

Table 1: the body weight gain of probiotic agent to pig compares

Table 1 data shows, experimental group pig energy for growth is apparently higher than control group, and between feeding period, experimental group is suffered from diarrhoea, the digestive tract diseases such as constipation obviously reduces, and stool odor also obviously reduces, and mosquitos and flies quantity is also few than control group, and economic benefit significantly improves.

Embodiment 3 processes waste water example effects

Get abundant rich production plant, extracting glutamic acid waste water, enter Sewage treatment systems according to embodiment 1 method still bed material, utilize 50L bucket as testing installation and be with stirring, getting 30L respectively, adding in two buckets, pH is adjusted to be 7.0, water temperature 20 DEG C, sampling and measuring COD, ammonia nitrogen, total nitrogen data; Control group does not add biotechnological formulation, experimental group adds biotechnological formulation in embodiment 1, add biotechnological formulation 20 grams by every cubic metre of still bed material at every turn, add 1 every day, after adding one week continuously, sampling and measuring COD, ammonia nitrogen, total nitrogen data, waste water after treatment reaches emission standard completely, and concrete data are in table 2:

Table 2

Group COD average removal rate Ammonia nitrogen average removal rate Control group 6.5% 8.2% Experimental group 98.7% 99.3%

What more than enumerate is only best specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.

Claims (1)

1. process a biotechnological formulation for glutamic acid fermentation waste water, it is characterized in that, described biotechnological formulation is that complex micro organism fungicide and carrier are mixed with according to the weight ratio of 1:1, and the activeconstituents of described complex micro organism fungicide comprises the raw material of following weight part:
10 parts, yeast, subtilis 7 parts, bacillus megaterium 6 parts, denitrifying bacteria 5 parts, pseudomonas aeruginosa 5 parts, rhodococcus 4 parts, Phanerochaete chrysosporium 3 parts;
Described carrier is chitosan;
Described yeast is yeast (Candida santamariae) CGMCC No:2959;
Described subtilis is subtilis (Bacillus subtilis) CGMCC No:2947;
Described bacillus megaterium is bacillus megaterium (Bacillus megatherium) CGMCC No:1487;
Described denitrifying bacteria is denitrifying bacteria (Paracoccus pantotrophus) ATCC 35512;
Described pseudomonas aeruginosa is pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC 15442;
Described rhodococcus be rhodococcus ( rhodococcus rhodochrous) ATCC 15906;
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725.
CN201410548050.3A 2014-10-16 2014-10-16 A kind of biotechnological formulation processing glutamic acid fermentation waste water CN104230004B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410548050.3A CN104230004B (en) 2014-10-16 2014-10-16 A kind of biotechnological formulation processing glutamic acid fermentation waste water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410548050.3A CN104230004B (en) 2014-10-16 2014-10-16 A kind of biotechnological formulation processing glutamic acid fermentation waste water

Publications (2)

Publication Number Publication Date
CN104230004A CN104230004A (en) 2014-12-24
CN104230004B true CN104230004B (en) 2015-10-14

Family

ID=52219025

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410548050.3A CN104230004B (en) 2014-10-16 2014-10-16 A kind of biotechnological formulation processing glutamic acid fermentation waste water

Country Status (1)

Country Link
CN (1) CN104230004B (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104891677B (en) * 2015-06-26 2016-08-24 内蒙古阜丰生物科技有限公司 A kind of preparation technology of the composite bacteria agent capable repairing Amino Acid Fermentation Wastewater
CN104961243B (en) * 2015-06-27 2017-02-15 内蒙古阜丰生物科技有限公司 Method for treating threonine fermentation wastewater by virtue of biochemical technology
CN104961244B (en) * 2015-07-02 2017-03-01 山东阜丰发酵有限公司 A kind of processing method of L arginine fermented waste fluid
CN105036330B (en) * 2015-07-02 2017-04-12 山东阜丰发酵有限公司 Preparation method of crystalline L-arginine alpha-ketoglutarate (AAKG)
CN105084557B (en) * 2015-09-05 2017-05-17 内蒙古阜丰生物科技有限公司 Technology for removing industrial COD and ammonia nitrogen from monosodium glutamate wastewater
CN105039228A (en) * 2015-09-05 2015-11-11 内蒙古阜丰生物科技有限公司 Biological agent for glutamate wastewater treatment
CN105174443B (en) * 2015-09-09 2017-05-17 呼伦贝尔东北阜丰生物科技有限公司 Treatment process for wastewater produced by extracting monosidum glutamate by concentrating isoelectric points
CN106119179A (en) * 2016-09-18 2016-11-16 天津北洋百川生物技术有限公司 A kind of preparation method of the complex micro organism fungicide for water body purification
CN107445391B (en) * 2017-04-19 2020-04-10 内蒙古阜丰生物科技有限公司 Method for repairing amino acid fermentation wastewater by using biochemical technology
CN106967644B (en) * 2017-04-19 2020-06-09 内蒙古阜丰生物科技有限公司 Biological agent for treating glutamic acid fermentation sewage
CN108504604A (en) * 2018-04-18 2018-09-07 北京高能时代环境技术股份有限公司 A kind of complex micro organism fungicide and its application in the aerobic reparation of landfill yard

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000001621A1 (en) * 1998-07-03 2000-01-13 Romero Jean Louis Biological means for sanitary drainage of polluted effluents and/or maintenance of sewer pipe system for evacuating said effluents
US6074558A (en) * 1998-11-16 2000-06-13 Bhp Minerals International Inc. Biochemical treatment of bitumen froth tailings
CN103031253A (en) * 2012-09-14 2013-04-10 上海环伟生物科技有限公司 Novel compound microorganism living bacteria agent, method for preparing same and applications
CN103864194A (en) * 2014-04-01 2014-06-18 山东省环境保护科学研究设计院 Emergency control method for sewage treatment system for treating abnormal influent/effluent quality
CN104030459A (en) * 2014-06-24 2014-09-10 天津科技大学 Microbial preparation for brackish water aquaculture, and preparation method and application thereof
CN104261631A (en) * 2014-10-14 2015-01-07 内蒙古阜丰生物科技有限公司 Environment-friendly process for treating glutamic acid fermentation wastewater

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000001621A1 (en) * 1998-07-03 2000-01-13 Romero Jean Louis Biological means for sanitary drainage of polluted effluents and/or maintenance of sewer pipe system for evacuating said effluents
US6074558A (en) * 1998-11-16 2000-06-13 Bhp Minerals International Inc. Biochemical treatment of bitumen froth tailings
CN103031253A (en) * 2012-09-14 2013-04-10 上海环伟生物科技有限公司 Novel compound microorganism living bacteria agent, method for preparing same and applications
CN103864194A (en) * 2014-04-01 2014-06-18 山东省环境保护科学研究设计院 Emergency control method for sewage treatment system for treating abnormal influent/effluent quality
CN104030459A (en) * 2014-06-24 2014-09-10 天津科技大学 Microbial preparation for brackish water aquaculture, and preparation method and application thereof
CN104261631A (en) * 2014-10-14 2015-01-07 内蒙古阜丰生物科技有限公司 Environment-friendly process for treating glutamic acid fermentation wastewater

Also Published As

Publication number Publication date
CN104230004A (en) 2014-12-24

Similar Documents

Publication Publication Date Title
Li et al. Microalgae-based wastewater treatment for nutrients recovery: a review
CN106190158B (en) A kind of soil-repairing agent and its production method for administering heavy metal pollution
CN104478098B (en) For the treatment of the microbial preparation of coal chemical industrial waste water
CN101306867B (en) High-efficiency purifier of biological enzyme
EP3095855B1 (en) Efficient bottom treatment bacillus, composite bottom treatment inoculant prepared using same and applications thereof
CN105802870A (en) Method for treatment of organic waste with microbial composite inoculant
CN101899397B (en) Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application
CN104528955B (en) A kind of microorganism formulation for repairing chemical fertilizer factory's sewage
CN101704691B (en) Bamboo residue organic fertilizer and method for preparing same
CN102616941B (en) Sea cucumber microecological water quality conditioning agent and method for preparing same
CN104140935B (en) One plant of production method and application of the Rhodococcus sp and its microbial inoculum with denitrification functions
CN101767893A (en) Device and method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater
JP5819442B2 (en) Biological treatment method of refractory wastewater and wastewater treatment agent
CN102212476A (en) Method for cleanly and efficiently producing microorganism bactericide
CN103122332B (en) Achromobacter xylosoxidans subsp.xylosoxidans LH-N25 and heterotrophic nitrification and aerobic denitrification microorganism bactericide and preparation method and use thereof
CN103373762A (en) Biological denitrification method for salt-containing sewage
CN102924134A (en) Alga microorganism fertilizer agent and preparation method thereof
CN103382057B (en) Method for treating cultivation wastewater
CN103274774B (en) High-efficiency biological liquid fertilizer taking livestock liquid dung as raw material and preparation method of fertilizer
CN104388348A (en) Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof
CN105420147A (en) Compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate
CN103864483B (en) Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal
CN102796673A (en) Feruloyl esterase production strain and method for producing feruloyl esterase by using same
CN102875210A (en) Method for co-composting of blue-green algae and polluted bottom sediment
CN101607809A (en) Microorganism concrete brick and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
GR01 Patent grant
C14 Grant of patent or utility model