CN104528955B - A kind of microorganism formulation for repairing chemical fertilizer factory's sewage - Google Patents

A kind of microorganism formulation for repairing chemical fertilizer factory's sewage Download PDF

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CN104528955B
CN104528955B CN201510038477.3A CN201510038477A CN104528955B CN 104528955 B CN104528955 B CN 104528955B CN 201510038477 A CN201510038477 A CN 201510038477A CN 104528955 B CN104528955 B CN 104528955B
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microorganism formulation
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sewage
microbe
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CN104528955A (en
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曹俊萍
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Hangzhou lubricant Science Equipment Co., Ltd.
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used

Abstract

The invention belongs to microbial technology field, disclose a kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms: step 1) prepare adsorbent, step 2) prepare microbe carbon source, step 3) prepare microbe carrier, step 4) prepare microorganism formulation.Microorganism formulation of the present invention can remove ammonia nitrogen, SS and aldehydes matter in chemical fertilizer factory's sewage effectively, and removal effect is preferable, reaches discharge standard.

Description

A kind of microorganism formulation for repairing chemical fertilizer factory's sewage
Technical field
The invention belongs to microbial technology field, disclose a kind of microorganism formulation for repairing chemical fertilizer factory's sewage and application thereof.
Background technology
Chemical fertilizer factory's sewage is the important sources of trade effluent, and the pollutant index that mainly exceeds standard is ammonia nitrogen, sulfide and aldehydes matter.In sewage, all kinds of pollutant loads are high, belong to high pollution thing sewage, and ammonia nitrogen concentration height can reach more than 1000mg/L up to 700mg/L, COD, and sulfide can reach 100mg/L, and phenols can reach 50mg/L.At present, method to high density pollution thing sewage disposal is divided into physico-chemical process, biochemical combination method, novel biological dinitrification method and traditional biological denitrogenation both at home and abroad.At present, more physico-chemical process, novel biological dinitrification method and traditional biological denitrogenation is studied.
Ammonia nitrogen is to cause body eutrophication and pollute the important polluter of one of environment, and in the almost all of Polluted water area of current China, ammonia nitrogen is all one of main pollutant.Body eutrophication has endangered all conglomeraties such as agricultural, fishery, tourist industry, also drinking water hygiene and food safety is constituted huge threat.Water pollution particularly body eutrophication has become as the influence factor that China's economic development is important.Often containing ammonia nitrogen in high density in chemical fertilizer industry sewage, it it is one of ammonia nitrogen in sewage main source.Therefore, how to remove the ammonia nitrogen in chemical fertilizer industry sewage and become a major issue in current Industrial Waste Water Treatments.Ammonia nitrogen is the major pollutants of chemical fertilizer factory's sewage, enters water body and can cause body eutrophication, causes water quality deterioration, make discharge severely limited.Chemical fertilizer factory's sewage is essentially from synthesis ammonia, the high concentration ammonia nitrogen sewage of urea plant, and it is inorganic ammonia that this part of contaminated water ammonia nitrogen is primarily present form.In sum, along with expanding economy, the ambient pressure that chemical fertilizer factory faces is increasingly strict, and for the needs of sustained economic development, in actual production, sewage effluent becomes giving top priority to what is the most important of chemical fertilizer factory's environmental problem.
For a long time, biochemical sewage processes adding of carbon source and uses methanol, white sugar (or cerelose) always, and methanol belongs to harmful influence, does not the most only exist the biggest danger;White sugar is relatively costly, and enterprise is the most beyond affordability.The microorganism formulation mechanism of action in terms of environmental conservation is mainly: pollutant oxidation is decomposed into the end-product such as carbon dioxide and water by metabolic response or is converted into the nutrient substance of microorganism by microbial inoculum; promote the growth and breeding of self, just as H2S can be changed into the element sulphur required for own growth by photosynthetic bacteria and bacillus cereus etc..Prior art processes the microorganism formulation of chemical fertilizer factory sewage, and mostly to there is bacteria microorganism compatibility unreasonable, and removal efficiency is low, and and relatively costly, enterprise is difficult to the defects such as acceptance, significantly hinders the application process of Biochemical method sewage.
Summary of the invention
The technical issues that need to address of the present invention are, in place of overcoming the deficiencies in the prior art, provide a kind of microorganism formulation for repairing chemical fertilizer factory's sewage and application thereof, this microorganism formulation comprises multiple bacterial strain, compatibility is reasonable, and synergism is strong, it is possible to effectively remove the ammonia nitrogen in sewage, sulfide, aldehydes matter and industry COD, low in input cost, can large-scale promotion use.
It is an object of the invention to be achieved through the following technical solutions:
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms: step 1) prepare adsorbent, step 2) prepare microbe carbon source, step 3) prepare microbe carrier, step 4) prepare microorganism formulation.
Specifically, described microorganism formulation is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain particle diameter zeolite powder between 50-100 mesh, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product;
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer;Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer;Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30 minutes, last natural cooling and get final product;The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100-200 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product;
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 20-22 DEG C, and dried water content is 10-12%, to obtain final product;The mass ratio of described adsorbent, microbe carrier carrier and microbe carbon source is 1: 2: 5.
Preferably,
Described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442;
Described enterococcus faecalis be enterococcus faecalis (Enterococcus faecalis) be ATCC 29212;
Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906;
Described Acinetobacter bauamnnii be Acinetobacter bauamnnii (Acinetobacter baumanii) be ATCC 19606;
The brilliant arthrobacterium of described one-tenth is for becoming brilliant arthrobacterium (Arthrobacter crystallopoietes) ATCC 15481;
Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842;
Described pseudomonas fluorescens is pseudomonas fluorescens (P.Fluorescens) ATCC 49642.
The particle diameter of described silicon dioxide or humic acids is 50-100 mesh.
The using method of mentioned microorganism preparation includes: first remove the float in sewage, subsequently into sedimentation tank, sedimentation tank is laid the fine sand of 50cm thickness, is precipitated 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7-7.5, adds 20 grams of microorganism formulations according to every cubic metre of liquid every time, adds 1 every day, after adding three days continuously, stand one week again, by liquid filtration sterilization, finally discharge.
Strain of the present invention all can be commercially available from commercial channel such as American Type Culture collection warehousing (ATCC).The amplification culture method of the bacterial strain of the present invention can record according to document or the record of prior art is carried out, and is not the most the innovative point of the present invention, as space is limited, repeats the most one by one.
The beneficial effect that the present invention obtains is primarily but not limited to the following aspects:
Reasonable compatibility between each strain in microorganism formulation of the present invention, symbiosis coordination, the most not antagonism, activity is high, and Biomass is big, and breeding is soon;The microorganism formulation of the present invention, by the various strains that can form dominant microflora, is configured to high-effect bacterial, is added in Waste Water Treatment by a certain amount of, accelerates the bacterial strain degraded to pollutant, to improve the biological treatment efficiency of system, it is ensured that system stable operation;Microorganism formulation of the present invention is suitable to chemical fertilizer factory's sewage disposal, can improve the process water yield and water quality treatment, reduces operating cost, promotes qualified discharge;The present invention is prepared for microbe carbon source nutriment, low price by agricultural wastes, is easily accept by enterprises for;The present invention with the addition of the adsorbent that the silicon dioxide of certain particle diameter, zeolite powder and humic acids are constituted, and can be used to assemble pollutant and thalline, improves the contact area of bacterial strain and pollutant, improves biological oxidation efficiency.
Detailed description of the invention
For the technical scheme making those skilled in the art be more fully understood that in the application, below in conjunction with the application specific embodiment, the technical scheme of the application is clearly and completely described, it is clear that, described embodiment is only some embodiments of the present application rather than whole embodiments.Based on the embodiment in the application, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all should belong to the scope of protection of the invention.
Embodiment 1
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain the zeolite powder that particle diameter is 50, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product;The particle diameter of described silicon dioxide or humic acids is 50 mesh.
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer;Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer;Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor at 95 DEG C, insulation reaction 30 minutes, last natural cooling and get final product;The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product;
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 20 DEG C, and dried water content is 10%, to obtain final product;The mass ratio of described adsorbent, microbe carrier carrier and microbe carbon source is 1: 2: 5.
The using method of mentioned microorganism preparation includes: first remove the float in sewage, subsequently into sedimentation tank, sedimentation tank is laid the fine sand of 50cm thickness, is precipitated 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7, adds 20 grams of microorganism formulations according to every cubic metre of liquid every time, adds 1 every day, after adding three days continuously, stand one week again, by liquid filtration sterilization, finally discharge.
Embodiment 2
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain the particle diameter zeolite powder at 100 mesh, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product;The particle diameter of described silicon dioxide or humic acids is 100 mesh;
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer;Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer;Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor at 100 DEG C, insulation reaction 30 minutes, last natural cooling and get final product;The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 200 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product;
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 22 DEG C, and dried water content is 12%, to obtain final product;The mass ratio of described adsorbent, microbe carrier carrier and microbe carbon source is 1: 2: 5.
Described micrococcus luteus be micrococcus luteus (Micrococcus luteus) ATCC 49442 (APPLIED AND ENVIRONMENTAL MICROBIOLOGY1992, p.3423-3425);
Described enterococcus faecalis be enterococcus faecalis (Enterococcus faecalis) be ATCC 29212 (In Vivo.2009 Jan-Feb;23 (1): 81-7.);
Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906 (J.Bacteriol.November 2001);
Described Acinetobacter bauamnnii be Acinetobacter bauamnnii (Acinetobacter baumanii) be ATCC 19606 (Infect Immun.2012Mar;80 (3): 1015-24);
The brilliant arthrobacterium of described one-tenth is finished product arthrobacterium (Arthrobacter crystallopoietes) ATCC 15481 (Appl.Environ.Microbiol.March 15,200975:61774-1777).
Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842 (J.Microbiol.Biotechnol. (2006), 16 (10), 1650-1655);
Described pseudomonas fluorescens is pseudomonas fluorescens (P.Fluorescens) ATCC49642 (WO2003066873A1).
The using method of mentioned microorganism preparation includes: first remove the float in sewage, subsequently into sedimentation tank, sedimentation tank is laid the fine sand of 50cm thickness, is precipitated 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7, adds 20 grams of microorganism formulations according to every cubic metre of liquid every time, adds 1 every day, after adding three days continuously, stand one week again, by liquid filtration sterilization, finally discharge.
Embodiment 3
With the sewage of this city chemical fertilizer factory as example, first remove the float in sewage, subsequently into sedimentation tank, the fine sand of 50cm thickness laid by sedimentation tank, precipitating 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7, add 20 grams of microorganism formulations according to every cubic metre of liquid every time, add every day 1 time, after adding three days continuously, then stand one week, by liquid filtration sterilization, finally discharge.Detection all takes three water samples every time, averages;The most each index all reaches preferable discharge standard, and the Testing index of sewage is shown in Table 1:
Table 1
Conclusion: microorganism formulation of the present invention can remove ammonia nitrogen, SS and aldehydes matter in chemical fertilizer factory's sewage effectively, removal effect is preferable, reaches discharge standard.
Although, the most illustrating by generality and detailed explanation has been made in this case by detailed description of the invention, but on the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, the amendment made without departing from theon the basis of the spirit of the present invention or improvement, belong to the scope of protection of present invention.

Claims (3)

1. the microorganism formulation being used for repairing chemical fertilizer factory's sewage, it is characterised in that described microorganism formulation is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain particle diameter zeolite powder between 50-100 mesh, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product;
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer;Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer;Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30 minutes, last natural cooling and get final product;The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100-200 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product;
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step 3) microbe carrier prepared, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 20-22 DEG C, and dried water content is 10-12%, to obtain final product;
Described step 4) in, the mass ratio of adsorbent, microbe carrier and microbe carbon source is 1: 2: 5.
Microorganism formulation the most according to claim 1, it is characterised in that described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442;Described enterococcus faecalis be enterococcus faecalis (Enterococcus faecalis) be ATCC 29212;Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906;Described Acinetobacter bauamnnii be Acinetobacter bauamnnii (Acinetobacter baumanii) be ATCC 19606;The brilliant arthrobacterium of described one-tenth is for becoming brilliant arthrobacterium (Arthrobacter crystallopoietes) ATCC 15481;Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842;Described pseudomonas fluorescens is pseudomonas fluorescens (P.Fluorescens) ATCC 49642.
Microorganism formulation the most according to claim 1, it is characterised in that the particle diameter of described silicon dioxide or humic acids is 50-100 mesh.
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