CN104891677A - Preparation technique of complex fungicide repairing amino acid fermenting wastewater - Google Patents
Preparation technique of complex fungicide repairing amino acid fermenting wastewater Download PDFInfo
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- CN104891677A CN104891677A CN201510360916.2A CN201510360916A CN104891677A CN 104891677 A CN104891677 A CN 104891677A CN 201510360916 A CN201510360916 A CN 201510360916A CN 104891677 A CN104891677 A CN 104891677A
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Abstract
The invention relates to the technical field of microorganism, in particular to a preparation technique of complex fungicide repairing amino acid fermenting wastewater. The preparation technique includes steps of 1) preparing a carrier; 2) preparing complex fungicide liquid; 3) preparing complex algae solution; 4) preparing complex fungicide. The preparation technique is simple, feasible, easy to master, and applicable to industrial popularization and use.
Description
Technical field
The present invention relates to microbial technology field, be specifically related to a kind of preparation technology repairing the composite fungus agent of Amino Acid Fermentation Wastewater.
Background technology
in the industrial production, be a kind of organic waste water of very high concentrations containing a large amount of amino acid whose waste water, have that organic concentration is high, ammonia nitrogen concentration is high, SO
4 2-or Cl
-the features such as concentration is high, pH is low, are difficult to process.After the process such as this waste water is separated through thalline, production yeast, anaerobically fermenting, aerobic aeration, although the COD of more than 90% can be removed
cr, but the COD of waste water
cr, ammonia-nitrogen content still do not reach the sewage drainage standard of national regulation.These organic waste waters quite a few directly drain into rivers, not only serious environment pollution, also the resource of preciousness is thrown away as waste; Meanwhile, high-concentration waste water causes Dissolved Oxygen in Water to reduce after entering water body, causes water quality deterioration, affects water ecological environment.
The problem processing waste water main is that one-time investment is excessive, or day-to-day operation expense is too high, and most producer cannot bear, the present situation of long term maintenance of having to discharge beyond standards.The previous research of applicant is by waste water as preparing fertilizer, and it is applicable to the enterprise possessing fertiliser production qualification and ability, but for other amino acids production enterprises, can not reach the object of producing fertilizer.At present, studying hotter is biotechnological formulation process waste water, and it possesses with low cost, can not cause the advantages such as secondary pollution, but it is unreasonable to possess microorganism compatibility, processing efficiency is low, and microbe carrier exists internal mass transfer difference, not easily printing opacity, the defects such as specific surface area is little, and work-ing life is short; Biotechnological formulation needs to throw in often, adds cost, and injected volume is large, and the sludge quantity of generation is large, is not easy process.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of this invention is to provide a kind of preparation technology repairing the composite fungus agent of Amino Acid Fermentation Wastewater.The present invention adopts following technical scheme to realize:
Repair a preparation technology for the composite fungus agent of Amino Acid Fermentation Wastewater, it comprises the steps: that step 1) prepares carrier, step 2) prepare composite bacteria liquid, step 3) prepares compound algae liquid, and step 4) prepares composite fungus agent;
Particularly, comprise the steps:
Step 1) prepares carrier:
A): take following each raw material according to weight part: water 20-22 part, humic acids 7-9 part, sucrose 6-8 part, kaolin 3-4 part, ammonium sulfate 3-4 part, chitin 2-3 part, cotton 2-3 part, silica-gel powder 1-2 part; Preferably, the particle diameter of silica-gel powder is 200 orders;
B): humic acids is pulverized, then adds kaolin, mix, be ground to 100 object humic acids kaolin powder;
C): by water, sucrose, ammonium sulfate, chitin, cotton and silica-gel powder add in stirred reactor successively, 200 turns/min stirs 10min, then add humic acids kaolin powder, 500 turns/min stirs 3min, leaves standstill 12 hours subsequently, finally be placed in 60 DEG C to dry to moisture content lower than 5%(parts by weight), obtain bio-carrier;
Step 2) prepare composite bacteria liquid:
By subtilis, Lactobacillus acidophilus, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, then mixes according to the volume ratio of 2:2:2:1, leaves standstill 6 hours;
Step 3) prepares compound algae liquid:
It is 1 × 10 that scenedesmus obliquus and chlorella are cultured to concentration respectively
5the algae liquid of individual/ml, then mixes according to the volume ratio of 1:1, leaves standstill 3 hours;
Step 4) prepares composite fungus agent;
By composite bacteria liquid, compound algae liquid and carrier according to the quality of 5:1:10 than mixing and stirring, room temperature leaves standstill 24-36h, dry in the air to water content be 10%(parts by weight), to obtain final product.
Bacterial classification of the present invention and algae all commercial sources can be bought and obtain from preservation center etc.
Bacterial classification of the present invention and algae all obtain bacterium liquid or the algae liquid of desired concn by the cultural method of routine, this is innovative point of the present invention not, as space is limited, does not repeat one by one.
The beneficial effect that the present invention obtains mainly comprises:
Preparation technology's simple possible of the present invention, those skilled in the art are easy to grasp, and are applicable to industrialization promotion and use;
Carrier provided by the invention, can not only expand the specific surface area of carrier, and have tensile strength large, be evenly distributed, specific surface area is large, the features such as long service life;
Carrier provided by the invention can improve the adhesion amount of microorganism greatly, and increase the biofilm biomass of overall attachment, microorganism concn is improved, and can reduce mud generation;
The existence of the anaerobism that carrier provided by the invention provides, anoxic and aerobic various environment can promote to promote the minimizing of mud by the treatment effect of microorganism simultaneously, is conducive to the removal of the pollutent such as ammonia nitrogen in waste water;
Composite fungus agent of the present invention, by various bacterial classification and the algae that can form dominant microflora, is mixed with high-performance bio preparation, reasonable compatibility between each microorganism, and symbiosis is coordinated, mutually not antagonism, and biomass is large, and breeding is fast.
Composite fungus agent of the present invention is suitable for Amino Acid Fermentation Wastewater process, and be convenient to transport and preserve, more easily activate during use, running cost is cheap.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, the technical scheme of the application is clearly and completely described, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
Repair a preparation technology for the composite fungus agent of Amino Acid Fermentation Wastewater, it comprises the steps:
Step 1) prepares carrier:
A): take following each raw material according to weight part: 20 parts, water, humic acids 7 parts, sucrose 6 parts, kaolin 3 parts, 3 parts, ammonium sulfate, chitin 2 parts, 2 parts, cotton, silica-gel powder 1 part;
B): humic acids is pulverized, then adds kaolin, mix, be ground to 100 object humic acids kaolin powder;
C): by water, sucrose, ammonium sulfate, chitin, cotton and silica-gel powder add in stirred reactor successively, 200 turns/min stirs 10min, then add humic acids kaolin powder, 500 turns/min stirs 3min, leaves standstill 12 hours subsequently, finally be placed in 60 DEG C to dry to moisture content lower than 5%(parts by weight), obtain bio-carrier;
Step 2) prepare composite bacteria liquid:
By subtilis, Lactobacillus acidophilus, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, then mixes according to the volume ratio of 2:2:2:1, leaves standstill 6 hours;
Step 3) prepares compound algae liquid:
It is 1 × 10 that scenedesmus obliquus and chlorella are cultured to concentration respectively
5the algae liquid of individual/ml, then mixes according to the volume ratio of 1:1, leaves standstill 3 hours;
Step 4) prepares composite fungus agent;
By composite bacteria liquid, compound algae liquid and carrier according to the quality of 5:1:10 than mixing and stirring, room temperature leaves standstill 24-36h, dry in the air to water content be 10%(parts by weight), to obtain final product.
Embodiment 2
Repair a preparation technology for the composite fungus agent of Amino Acid Fermentation Wastewater, it comprises the steps:
Step 1) prepares carrier:
A): take following each raw material according to weight part: 22 parts, water, humic acids 9 parts, sucrose 8 parts, kaolin 4 parts, 4 parts, ammonium sulfate, chitin 3 parts, 3 parts, cotton, silica-gel powder 2 parts; Wherein, the particle diameter of silica-gel powder is 200 orders;
B): humic acids is pulverized, then adds kaolin, mix, be ground to 100 object humic acids kaolin powder;
C): by water, sucrose, ammonium sulfate, chitin, cotton and silica-gel powder add in stirred reactor successively, 200 turns/min stirs 10min, then add humic acids kaolin powder, 500 turns/min stirs 3min, leaves standstill 12 hours subsequently, finally be placed in 60 DEG C to dry to moisture content lower than 5%(parts by weight), obtain bio-carrier;
Step 2) prepare composite bacteria liquid:
By subtilis, Lactobacillus acidophilus, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, then mixes according to the volume ratio of 2:2:2:1, leaves standstill 6 hours;
Step 3) prepares compound algae liquid:
It is 1 × 10 that scenedesmus obliquus and chlorella are cultured to concentration respectively
5the algae liquid of individual/ml, then mixes according to the volume ratio of 1:1, leaves standstill 3 hours, to obtain final product;
Step 4) prepares composite fungus agent;
By composite bacteria liquid, compound algae liquid and carrier according to the quality of 5:1:10 than mixing and stirring, room temperature leaves standstill 24-36h, dry in the air to water content be 10%(parts by weight), to obtain final product.
Wherein, subtilis is (Bacillus subtilis) CGMCC NO:2947 (can see CN101838621A); Lactobacillus acidophilus is that (Lactobacillus acidophilus) ATCC4356 (can see Appl. Environ. Microbiol.December 2008 vol. 74; no. 24 7824-7827), Acinetobacter bauamnnii is (Acinetobacter baumanii) for ATCC 19606(can see Infect Immun. 2012 Mar; 80 (3): 1015-24), Sphingomonas is that (Sphingomonas sp.) CGMCC NO.4589(can see CN102168054A), scenedesmus obliquus is that (Scenedesmus obliquus) CGMCC No.8015(can see CN103484374A), chlorella is (Chlorella sp.) CGMCC NO:3037(CN101565675A).
Embodiment 3
The water treatment effect test of composite fungus agent prepared by the embodiment of the present invention 2:
With the fermentation waste water removing the materials such as fermentation thalli, polysaccharide and polysaccharide for processing sample is tested.
Control group 1: use sawdust carrier; Microorganism type is identical with test group; Processing mode: after adding three days continuously, every day 5g/ cubic meter, then leave standstill six days, check processing the results are shown in Table 1.
Control group 2: carrier is identical with embodiment 2, difference is, only adds four kinds of bacterium, does not add compound algae liquid; Processing mode: first day adds 5g/ cubic meter, then leave standstill six days, check processing the results are shown in Table 1.
Test group: composite fungus agent prepared by the embodiment of the present invention 2; Processing mode: after adding one day, 5g/ cubic meter, then leave standstill six days, check processing the results are shown in Table 1.
Table 1
| Before process (mg/L) | After process (control group 1) (mg/L) | After process (control group 2) (mg/L) | (test group) (mg/L) after process | |
| COD | 1217 | 13.3 | 40.6 | 6.2 |
| NH3-N | 204 | 10.6 | 17.8 | 5.3 |
| Sulfide | 27 | 4.3 | 3.5 | 1.1 |
| Chlorion | 6.2 | 1.3 | 1.9 | 0.3 |
Conclusion: carrier prepared by the present invention is large empty reticulated structure, and be twice above than the specific surface area of the sawdust carrier of routine, microorganic adhesion is effective, and drop into number of times and weight and all reduce, long service life, without the need to repeatedly throwing in, save cost, reduce the generation of mud.Mushroom and algae reasonable compatibility in composite fungus agent prepared by the present invention, effectively can remove the pollutents such as ammonia nitrogen, sulfide and the muriate in fermentation waste water, reach emission standard completely.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (2)
1. repair a preparation technology for the composite fungus agent of Amino Acid Fermentation Wastewater, it is characterized in that, described technique comprises the steps: that step 1) prepares carrier, step 2) prepare composite bacteria liquid, step 3) prepares compound algae liquid, and step 4) prepares composite fungus agent.
2. technique according to claim 1, is characterized in that, described technique specifically comprises the steps:
Step 1) prepares carrier:
A): take following each raw material according to weight part: water 20-22 part, humic acids 7-9 part, sucrose 6-8 part, kaolin 3-4 part, ammonium sulfate 3-4 part, chitin 2-3 part, cotton 2-3 part, silica-gel powder 1-2 part; Preferably, the particle diameter of silica-gel powder is 200 orders;
B): humic acids is pulverized, then adds kaolin, mix, be ground to 100 object humic acids kaolin powder;
C): by water, sucrose, ammonium sulfate, chitin, cotton and silica-gel powder add in stirred reactor successively, 200 turns/min stirs 10min, then add humic acids kaolin powder, 500 turns/min stirs 3min, leaves standstill 12 hours subsequently, finally be placed in 60 DEG C dry to moisture content lower than 5%, obtain bio-carrier;
Step 2) prepare composite bacteria liquid:
By subtilis, Lactobacillus acidophilus, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, then mixes according to the volume ratio of 2:2:2:1, leaves standstill 6 hours;
Step 3) prepares compound algae liquid:
It is 1 × 10 that scenedesmus obliquus and chlorella are cultured to concentration respectively
5the algae liquid of individual/ml, then mixes according to the volume ratio of 1:1, leaves standstill 3 hours;
Step 4) prepares composite fungus agent;
By composite bacteria liquid, compound algae liquid and carrier according to the quality of 5:1:10 than mixing and stirring, room temperature leaves standstill 24-36h, and drying in the air to water content is 10%, to obtain final product.
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| CN201510360916.2A CN104891677B (en) | 2015-06-26 | 2015-06-26 | A kind of preparation technology of the composite bacteria agent capable repairing Amino Acid Fermentation Wastewater |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106045626A (en) * | 2016-06-07 | 2016-10-26 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for producing biological bacterial fertilizer from amino acid fermentation liquid waste and corn stalks |
| CN113402124A (en) * | 2021-07-02 | 2021-09-17 | 内蒙古阜丰生物科技有限公司 | Method for reducing blood sugar and collecting salt of amino acid wastewater |
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| JPS6014996A (en) * | 1983-07-07 | 1985-01-25 | Tax Adm Agency | Treatment of waste water produced in food manufacture and the like |
| CN104230004A (en) * | 2014-10-16 | 2014-12-24 | 内蒙古阜丰生物科技有限公司 | Biological agent for processing glutamic acid fermentation waste water |
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2015
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| JPS6014996A (en) * | 1983-07-07 | 1985-01-25 | Tax Adm Agency | Treatment of waste water produced in food manufacture and the like |
| CN104261570A (en) * | 2014-09-16 | 2015-01-07 | 福建省农业科学院农业工程技术研究所 | Livestock and poultry breeding anaerobic wastewater purifying agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106045626A (en) * | 2016-06-07 | 2016-10-26 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for producing biological bacterial fertilizer from amino acid fermentation liquid waste and corn stalks |
| CN113402124A (en) * | 2021-07-02 | 2021-09-17 | 内蒙古阜丰生物科技有限公司 | Method for reducing blood sugar and collecting salt of amino acid wastewater |
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| CN104891677B (en) | 2016-08-24 |
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