CN104894033A - Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant - Google Patents
Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant Download PDFInfo
- Publication number
- CN104894033A CN104894033A CN201510355997.7A CN201510355997A CN104894033A CN 104894033 A CN104894033 A CN 104894033A CN 201510355997 A CN201510355997 A CN 201510355997A CN 104894033 A CN104894033 A CN 104894033A
- Authority
- CN
- China
- Prior art keywords
- sewage
- fungus agent
- composite fungus
- compound microbial
- microbial inoculant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 230000000813 microbial effect Effects 0.000 title abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 title abstract description 11
- 239000001301 oxygen Substances 0.000 title abstract description 11
- 239000000126 substance Substances 0.000 title abstract description 9
- 150000001875 compounds Chemical class 0.000 title abstract 8
- 239000002054 inoculum Substances 0.000 title abstract 8
- 230000000593 degrading effect Effects 0.000 title abstract 2
- 239000010865 sewage Substances 0.000 claims abstract description 55
- 241000894006 Bacteria Species 0.000 claims abstract description 45
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 17
- 241000222173 Candida parapsilosis Species 0.000 claims abstract description 16
- 241000222178 Candida tropicalis Species 0.000 claims abstract description 16
- 229940055022 candida parapsilosis Drugs 0.000 claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 59
- 239000002131 composite material Substances 0.000 claims description 59
- 241000233866 Fungi Species 0.000 claims description 58
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 19
- 235000014655 lactic acid Nutrition 0.000 claims description 14
- 210000000936 intestine Anatomy 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 239000005416 organic matter Substances 0.000 abstract description 7
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 244000063299 Bacillus subtilis Species 0.000 abstract 2
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 2
- 241000194032 Enterococcus faecalis Species 0.000 abstract 2
- 241000186660 Lactobacillus Species 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 229940032049 enterococcus faecalis Drugs 0.000 abstract 2
- 229940039696 lactobacillus Drugs 0.000 abstract 2
- 239000000149 chemical water pollutant Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 35
- 238000000034 method Methods 0.000 description 22
- 244000005700 microbiome Species 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 239000000945 filler Substances 0.000 description 7
- 238000012545 processing Methods 0.000 description 6
- 238000010276 construction Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- 239000010802 sludge Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 244000005706 microflora Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000004400 mucous membrane Anatomy 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108010001478 Bacitracin Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003416 augmentation Effects 0.000 description 1
- 108700023668 bacilysin Proteins 0.000 description 1
- 229960003071 bacitracin Drugs 0.000 description 1
- 229930184125 bacitracin Natural products 0.000 description 1
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a compound microbial inoculant for degrading chemical oxygen demand and a preparation method of the compound microbial inoculant. The compound microbial inoculant comprises, by volume, 9-11% of candida tropicalis, 9-11% of candida parapsilosis, 28-32% of bacillus subtilis, 28-32% of bacillus licheniformis and 19-21% of enterococcus faecalis lactobacillus. The preparation method of the compound microbial inoculant includes that the candida tropicalis, the candida parapsilosis, the bacillus subtilis, the bacillus licheniformis and the enterococcus faecalis lactobacillus are respectively inoculated and cultured in sewage culturing media to obtain respective bacterium solutions, and the bacterium solutions are mixed according to the volume ratio to obtain the compound microbial inoculant, wherein the sewage culturing media are abundant in organic matter after being sterilized. The compound microbial inoculant is high in environment adaptability, applicable to microbial enhanced sewage treatment in different areas and various sewage including aquaculture sewage, domestic sewage, landfill leachate and the like which are abundant in organic matter, and capable of removing 95% of the COD in sewage.
Description
Technical field
The present invention relates to microbiobacterial agent field, specifically the composite fungus agent and preparation method thereof of a kind of COD that degrades.
Background technology
Well-known, current mankind is faced with multiple crisis, such as crisis in food, energy shortage, resource scarcity, ecological degeneration and Population explosion etc.After the mankind enter 21 century, utilize the unlimited Biological resources epoch and a series of new problems produced by running into from utilizing the limited mineral wealth epoch to be transitioned into.Because microorganism cells is that one larger than face amount, biochemical conversion ability strong, can carries out the Living system of quick self-replacation; and they also have the diversity of species, heredity, metabolism and ecotype, make them can play its irreplaceable unique effect in the various crises solving facing mankind.
Industrial pollution source: trade effluent is the important source of pollution in waters, the features such as the amount of having is large, area is wide, complicated component, toxicity are large, should not purify, difficult.Agricultural pollution source: agricultural pollution source comprises excrement of animals, agricultural chemicals, chemical fertilizer etc.China is one of country that soil erosion is the fastest in the world, every chronology soil number of dropouts about 5,000,000,000 tons, the lake of 2/3rds is subject to the harm of eutrophication in various degree, causes algae and other biological distorted proliferation, cause the change of water transparency and dissolved oxygen, thus cause water quality deterioration.Domestic pollution source: the various washing composition that domestic pollution source mainly uses in urban life and sewage, rubbish, ight soil etc., mostly be nontoxic inorganic salts, many containing nitrogen phosphate and sulfur in sanitary sewage, bacterium of curing the disease is many.Existing sewage disposal process by activated sludge process cost is high, and COD (chemical oxygen demand (COD), Chemical Oxygen Demand) clearance is undesirable.
Microbial preparation was existing in water surrounding improvement in recent years applied, but there is a lot of problem, as some microorganisms can not produce enzyme, the small molecules nutritive substance of just simple absorption water body, effectively can not promote the material cycle of water body environment, effectiveness comparison is slow.Bacterial classification is single, and the effect of each microorganism in the link of water body material cycle is different, and show through experiment, composite bacteria effect and processing efficiency are much larger than single culture.
Summary of the invention
The object of the present invention is to provide that a kind of adaptive capacity to environment is strong, COD of sewage is removed and can be reached composite fungus agent of the degraded COD of 95% and preparation method thereof, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
To degrade a composite fungus agent of COD, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 9-11%, Candida parapsilosis 9-11%, subtilis 28-32%, Bacillus licheniformis 28-32%, excrement intestines milk-acid bacteria 19-21%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10
8-5.4 × 10
8individual/ml, Candida parapsilosis 2.3 × 10
8-5.8 × 10
8individual/ml, subtilis 5.5 × 10
8-9.6 × 10
8individual/ml, Bacillus licheniformis 5.0 × 10
8-9.8 × 10
8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10
8-2.6 × 10
8individual/ml.
As the further scheme of the present invention: the composite fungus agent of described degraded COD, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 10%, Candida parapsilosis 10%, subtilis 30%, Bacillus licheniformis 30%, excrement intestines milk-acid bacteria 20%.
The preparation method of the composite fungus agent of described degraded COD, is made up of following steps:
One, cultivate in organic abundant sewage substratum after candida tropicalis, Candida parapsilosis, subtilis, Bacillus licheniformis, excrement intestines milk-acid bacteria being inoculated in sterilizing respectively, obtain respective bacterium liquid; Be 30 DEG C in temperature, shaking speed is that the bacterium number being cultured to bacterium liquid under the condition of 200r/min reaches above-mentioned scope;
Two, above-mentioned bacterium liquid is mixed according to volume ratio, obtain composite fungus agent.
As the further scheme of the present invention: the steam sterilizing condition that step one prepares sewage in the sewage substratum of bacterium liquid is 121 DEG C, 30min.
As the further scheme of the present invention: the working conditions of composite fungus agent requires that sewage pH is 6.0-7.0, temperature 25-35 DEG C.
Strain characteristic selected by the present invention and function as follows:
1) subtilis, can produce amylase and bacillin, again can the organic matter such as degradation of polysaccharide class.
2) Bacillus licheniformis, can produce proteolytic enzyme, bacitracin etc., adapts to all kinds of Sewage Environment ability strong.
3) candida tropicalis, Candida parapsilosis, can produce proteolytic enzyme, and degraded multiple protein class is organic.
4) sausage stuffed mainly with bean starch paste milk-acid bacteria, can produce lactic acid class goods and materials, and the multiple macromole of effective acidication is organic, release small organic molecule matter.
Compared with prior art, the invention has the beneficial effects as follows: composite fungus agent prepared by the present invention adaptive capacity to environment is strong, be applicable to different areas microbial augmentation sewage disposal, be applicable to the sewage that all kinds of organic matters such as aquaculture wastewater, sanitary sewage and percolate class sewage are abundant, COD of sewage is removed can reach 95%.Solve existing sewage disposal process by activated sludge process cost high, the problem that COD clearance is undesirable.Described in specific as follows.
1) microorganism used can the multiple larger molecular organics of efficient degradation.Traditional active sludge contains multiple-microorganism, but superior microorganism Microflora is limited, can not reach the object of efficient degradation, and sludge sump laid down cost is high, and operation maintenance cost is high, to sewage organic matter degradation limited efficacy.
2) microorganism used is based on sewage ancestral home bacterium
Microorganism has certain environmental compatibility as all biologies, traditional microorganism great majority used are Lu Sheng, reach water body and can not adapt to water surrounding, therefore can not breed very soon, form dominant microflora, and ancestral home bacterium is separated from Sewage Environment, to Sewage Environment strong adaptability, artificial input can form dominant microflora rapidly later.
3) microorganism used can also produce microbiotic sample material
Selected microorganism can produce microbiotic sample material as genus bacillus, Bacillus licheniformis, has restraining effect to pathogenic bacterium.
4) microorganism selected by is the mixture of multiple-microorganism, and they have stronger specific aim and combination ability, not mutually exclusive in biologic packing material, mutually coordinates in sewage, and successful improves.Do not need complicated engineering process during use, directly throw in, easy to use.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, aquaculture wastewater process.
1. implement place: Jingshan Area of Hubei plant.
2. water treatment amount: average treatment sewage at 100d/T, process water be animal manure just, higher containing organic matter.
3. using method and technique: utilize composite fungus agent technical finesse sewage.
Concrete treating processes is as follows; Sewage, after anaerobic pond fermentation, through piping from entering Aerobic Pond, at Aerobic Pond aeration, increasing the dissolved oxygen in sewage, making dissolved oxygen reach more than 2ppm.Add artificial bio-membrane's filler at Aerobic Pond, sewage is at the microbial film through there being a large amount of composite fungus agent to be formed, and the nutritive substance in water body is fully absorbed by the composite fungus agent on microbial film, then treated water, flows out through secondary filtration pond.
4. the addition of composite fungus agent: the composite fungus agent on initial stage filler is not also formed before a large amount of adhesion, average water per ton adds composite fungus agent 1 kilogram, composite fungus agent is adsorbed on above filler, after within general about 7 days, having a large amount of composite fungus agent mucous membrane to be formed, just no longer add composite fungus agent, composite fungus agent film is aging come off after, start again to add composite fungus agent.Described composite fungus agent, is made up of the following bacterium liquid according to volume ratio: candida tropicalis 9%, Candida parapsilosis 11%, subtilis 32%, Bacillus licheniformis 28%, excrement intestines milk-acid bacteria 20%.
5. average cost of water treatment is less than 0.3 yuan, and maintenance cost is low.
6. whole engineering of water treatment reduced investment expense is comparatively large, and according to a conventional method, the construction investment processing sewage per ton is at 900 yuan/d, and the construction investment processing sewage per ton declines 100 yuan/ton than traditional method.
7. treatment effect is as follows
During actual motion, composite fungus agent has good place to go effect to pollutent.Composite fungus agent filter tank influent COD mean concns is respectively: 1000.05mg/L, and water outlet water mean concns is respectively: 98.5g/L, and the clearance of dye thing is respectively 90.15%, reaches national grade one discharge standard.
Embodiment 2
In the embodiment of the present invention, town domestic sewage process
1. implement place: sewage farm, Jiangxia.
2. water treatment amount: average treatment sewage is at 200d/T.Process water is town domestic sewage, higher containing organic matter.
3. using method and technique: utilize composite fungus agent technical finesse sewage.
Concrete treating processes is as follows; Sewage, after anaerobic pond fermentation, through piping from entering Aerobic Pond, at Aerobic Pond aeration, increasing the dissolved oxygen in sewage, making dissolved oxygen reach more than 2ppm.Add artificial bio-membrane's filler at Aerobic Pond, sewage is at the microbial film through there being a large amount of composite fungus agent to be formed, and the nutritive substance in water body is fully absorbed by the composite fungus agent on microbial film, then treated water, flows out through filter tank.
4. the addition of composite fungus agent: the composite fungus agent on initial stage filter membrane is not also formed before a large amount of adhesion, average water per ton adds composite fungus agent 1.5 kilograms, composite fungus agent is adsorbed on above filter membrane, a large amount of composite fungus agent within about general 7-8 days, is had to stick after film formed, just no longer add composite fungus agent, composite fungus agent film is aging come off after, start again to add composite fungus agent.Composite fungus agent, is made up of the following bacterium liquid according to volume ratio: candida tropicalis 11%, Candida parapsilosis 9%, subtilis 28%, Bacillus licheniformis 32%, excrement intestines milk-acid bacteria 20%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10
8-5.4 × 10
8individual/ml, Candida parapsilosis 2.3 × 10
8-5.8 × 10
8individual/ml, subtilis 5.5 × 10
8-9.6 × 10
8individual/ml, Bacillus licheniformis 5.0 × 10
8-9.8 × 10
8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10
8-2.6 × 10
8individual/ml.
5. average cost of water treatment is less than 0.3 yuan, and maintenance cost is low.
6. whole engineering of water treatment reduced investment expense is comparatively large, and according to a conventional method, the construction investment processing sewage per ton is at 900 yuan/d, and the construction investment processing sewage per ton declines 110 yuan/ton than traditional method.
7. treatment effect is as follows:
During actual motion, composite fungus agent has good place to go effect to pollutent.Composite fungus agent filter tank influent COD mean concns is respectively: 1400.05mg/L, and water outlet water mean concns is respectively: 95.5g/L, and the clearance of dye thing is respectively 93.18%, reaches national grade one discharge standard.
Embodiment 3
In the embodiment of the present invention, certain food factory effluent process
1. implement place: Wuhan food company sewage farm.
2. water treatment amount: average treatment sewage is at 400d/T.Process water be animal manure just, higher containing organic matter.
3. using method and technique: utilize composite fungus agent technical finesse sewage.
Concrete treating processes is as follows; Sewage, after anaerobic pond fermentation, through piping from entering Aerobic Pond, at Aerobic Pond aeration, increasing the dissolved oxygen in sewage, making dissolved oxygen reach more than 2ppm.Add artificial bio-membrane's filler at Aerobic Pond, sewage is at the microbial film through there being a large amount of composite fungus agent to be formed, and the nutritive substance in water body is fully absorbed by the composite fungus agent on microbial film, then treated water, flows out through filter tank.
4. the addition of composite fungus agent: the composite fungus agent on initial stage filler is not also formed before a large amount of adhesion, average water per ton adds composite fungus agent 1 kilogram, composite fungus agent is adsorbed on above filler, after within general about 7 days, having a large amount of composite fungus agent mucous membrane to be formed, just no longer add composite fungus agent, composite fungus agent film is aging come off after, start again to add composite fungus agent.Composite fungus agent, is made up of the following bacterium liquid according to volume ratio: candida tropicalis 10%, Candida parapsilosis 10%, subtilis 30%, Bacillus licheniformis 30%, excrement intestines milk-acid bacteria 20%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10
8-5.4 × 10
8individual/ml, Candida parapsilosis 2.3 × 10
8-5.8 × 10
8individual/ml, subtilis 5.5 × 10
8-9.6 × 10
8individual/ml, Bacillus licheniformis 5.0 × 10
8-9.8 × 10
8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10
8-2.6 × 10
8individual/ml.
5. average cost of water treatment is less than 0.3 yuan, and maintenance cost is low.
6. whole engineering of water treatment reduced investment expense is comparatively large, and according to a conventional method, the construction investment processing sewage per ton declines 130 yuan/ton than traditional method.
7. treatment effect is as follows:
During actual motion, composite fungus agent has good place to go effect to pollutent.The aerobic influent COD mean concns of composite fungus agent is respectively: 1600.05mg/L, and water outlet water mean concns is respectively: 92.5g/L, and the clearance of pollutent is respectively 94.22%, reaches national grade one discharge standard.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
In addition, be to be understood that, although this specification sheets is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets is only for clarity sake, those skilled in the art should by specification sheets integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (5)
1. the composite fungus agent of the COD that degrades, it is characterized in that, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 9-11%, Candida parapsilosis 9-11%, subtilis 28-32%, Bacillus licheniformis 28-32%, excrement intestines milk-acid bacteria 19-21%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10
8-5.4 × 10
8individual/ml, Candida parapsilosis 2.3 × 10
8-5.8 × 10
8individual/ml, subtilis 5.5 × 10
8-9.6 × 10
8individual/ml, Bacillus licheniformis 5.0 × 10
8-9.8 × 10
8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10
8-2.6 × 10
8individual/ml.
2. the composite fungus agent of degraded COD according to claim 1, it is characterized in that, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 10%, Candida parapsilosis 10%, subtilis 30%, Bacillus licheniformis 30%, excrement intestines milk-acid bacteria 20%.
3. a preparation method for the composite fungus agent of the degraded COD as described in as arbitrary in claim 1-2, is characterized in that, be made up of following steps:
One, cultivate in organic abundant sewage substratum after candida tropicalis, Candida parapsilosis, subtilis, Bacillus licheniformis, excrement intestines milk-acid bacteria being inoculated in sterilizing respectively, obtain respective bacterium liquid; Be 30 DEG C in temperature, shaking speed is that the bacterium number being cultured to bacterium liquid under the condition of 200r/min reaches above-mentioned scope;
Two, above-mentioned bacterium liquid is mixed according to volume ratio, obtain composite fungus agent.
4. the preparation method of the composite fungus agent of degraded COD according to claim 3, it is characterized in that, the steam sterilizing condition that step one prepares sewage in the sewage substratum of bacterium liquid is 121 DEG C, 30min.
5. according to the composite fungus agent of the arbitrary described degraded COD of claim 1-2, it is characterized in that, the working conditions of composite fungus agent requires that sewage pH is 6.0-7.0, temperature 25-35 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510355997.7A CN104894033A (en) | 2015-06-25 | 2015-06-25 | Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510355997.7A CN104894033A (en) | 2015-06-25 | 2015-06-25 | Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104894033A true CN104894033A (en) | 2015-09-09 |
Family
ID=54026994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510355997.7A Pending CN104894033A (en) | 2015-06-25 | 2015-06-25 | Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104894033A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176902A (en) * | 2015-09-22 | 2015-12-23 | 宁波创蓝环境科技有限公司 | Microbial preparation for urban sewage treatment and application thereof |
| CN106630190A (en) * | 2016-12-20 | 2017-05-10 | 深圳市禹邦水处理技术有限公司 | COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof |
| CN108342338A (en) * | 2018-01-15 | 2018-07-31 | 杭州秀川科技有限公司 | A kind of processing method of the pharmacy waste water containing antibiotics |
| CN108441444A (en) * | 2018-03-22 | 2018-08-24 | 中电建水环境治理技术有限公司 | A kind of complex micro organism fungicide administered suitable for black and odorous water |
| CN109266573A (en) * | 2018-09-06 | 2019-01-25 | 中海新世纪量子生物科技（深圳）有限责任公司 | Landfill leachate and reverse osmosis concentrated liquid processing leavening, microbial inoculum and processing method |
| CN110438040A (en) * | 2019-07-22 | 2019-11-12 | 南京润中生物技术有限公司 | A kind of lichem bacillus strain and its application |
| CN112760255A (en) * | 2021-01-22 | 2021-05-07 | 湖北凌卓生物工程有限公司 | Compound microbial inoculum for degrading COD (chemical oxygen demand) and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257131A (en) * | 1998-12-16 | 2000-06-21 | 东北大学 | Technology for producing rare earth barium silicide alloy by carbon thermal reduction method |
| CN101475265A (en) * | 2009-01-16 | 2009-07-08 | 中国科学院南京土壤研究所 | Water purification method for circulating water industrialized aquiculture system and complex bacterial agent thereof |
| CN102286376A (en) * | 2011-06-21 | 2011-12-21 | 湖南省微生物研究所 | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof |
| CN102559545A (en) * | 2011-12-15 | 2012-07-11 | 北京大北农科技集团股份有限公司 | Composite micro-ecological preparation for aquatic product and premix thereof |
| CN102696923A (en) * | 2012-06-15 | 2012-10-03 | 天津昌农科技有限责任公司 | Premix compound for aquatic products, application thereof in feed additive, and batch thereof |
-
2015
- 2015-06-25 CN CN201510355997.7A patent/CN104894033A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257131A (en) * | 1998-12-16 | 2000-06-21 | 东北大学 | Technology for producing rare earth barium silicide alloy by carbon thermal reduction method |
| CN101475265A (en) * | 2009-01-16 | 2009-07-08 | 中国科学院南京土壤研究所 | Water purification method for circulating water industrialized aquiculture system and complex bacterial agent thereof |
| CN102286376A (en) * | 2011-06-21 | 2011-12-21 | 湖南省微生物研究所 | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof |
| CN102559545A (en) * | 2011-12-15 | 2012-07-11 | 北京大北农科技集团股份有限公司 | Composite micro-ecological preparation for aquatic product and premix thereof |
| CN102696923A (en) * | 2012-06-15 | 2012-10-03 | 天津昌农科技有限责任公司 | Premix compound for aquatic products, application thereof in feed additive, and batch thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘国防等: "油脂废水生物处理研究进展", 《应用生态学》 * |
| 陈谦等: "用于水产养殖的微生态制剂的研究和应用进展", 《应用与环境生物学报》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176902A (en) * | 2015-09-22 | 2015-12-23 | 宁波创蓝环境科技有限公司 | Microbial preparation for urban sewage treatment and application thereof |
| CN106630190A (en) * | 2016-12-20 | 2017-05-10 | 深圳市禹邦水处理技术有限公司 | COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof |
| CN106630190B (en) * | 2016-12-20 | 2020-06-16 | 永旌(湖北)环境技术有限公司 | Fungus agent for degrading COD and preparation method thereof |
| CN108342338A (en) * | 2018-01-15 | 2018-07-31 | 杭州秀川科技有限公司 | A kind of processing method of the pharmacy waste water containing antibiotics |
| CN108342338B (en) * | 2018-01-15 | 2021-01-19 | 杭州秀川科技有限公司 | Method for treating pharmaceutical wastewater containing antibiotics |
| CN108441444A (en) * | 2018-03-22 | 2018-08-24 | 中电建水环境治理技术有限公司 | A kind of complex micro organism fungicide administered suitable for black and odorous water |
| CN108441444B (en) * | 2018-03-22 | 2020-09-25 | 中电建生态环境集团有限公司 | Compound microbial agent suitable for black and odorous water treatment |
| CN109266573A (en) * | 2018-09-06 | 2019-01-25 | 中海新世纪量子生物科技（深圳）有限责任公司 | Landfill leachate and reverse osmosis concentrated liquid processing leavening, microbial inoculum and processing method |
| CN110438040A (en) * | 2019-07-22 | 2019-11-12 | 南京润中生物技术有限公司 | A kind of lichem bacillus strain and its application |
| CN110438040B (en) * | 2019-07-22 | 2022-06-03 | 南京润中环境科技有限公司 | Bacillus licheniformis strain and application thereof |
| CN112760255A (en) * | 2021-01-22 | 2021-05-07 | 湖北凌卓生物工程有限公司 | Compound microbial inoculum for degrading COD (chemical oxygen demand) and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102250768B (en) | Method for preparing enzyme and bacterium composite agent for treating sewage and sludge | |
| CN101306867B (en) | High-efficiency purifier of biological enzyme | |
| CN104894033A (en) | Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant | |
| CN105368745B (en) | Composite microbial preparation for treating black and odorous rivers and preparation method thereof | |
| CN105060506B (en) | A kind of microbe stuffing and its preparation method and application | |
| CN101787350B (en) | Functional composite biological agent for high-efficiency degradation of household refuses and application thereof | |
| CN105802870A (en) | Method for treatment of organic waste with microbial composite inoculant | |
| CN102229448B (en) | Purification microorganism bacterium ball as well as preparation and application thereof | |
| CN102875210A (en) | Method for co-composting of blue-green algae and polluted bottom sediment | |
| CN111705018B (en) | Yellow river halomonas capable of degrading organic matters and full salt in high-salinity wastewater and application thereof | |
| CN1348987A (en) | Prepn of microbial colony sludge for efficient treatment of sewage | |
| CN115353986B (en) | Belaisi bacillus strain WB strain and application thereof for treating swine wastewater | |
| CN105000674B (en) | Method used for processing environmental geology industrial wastewater via biotechnology | |
| CN103114062B (en) | Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof | |
| CN105130013A (en) | Method of treating piggery methane fluid by utilizing composite photosynthetic bacteria | |
| CN106587559A (en) | Sludge anaerobic digestion method | |
| CN104071902B (en) | Method for treating household wastewater | |
| CN101701197B (en) | Novel microorganism flora mixture and mixed nutrient medium thereof | |
| CN103937713B (en) | Microbiological deterioration liquid bacterial agent of food source organic waste and preparation method thereof | |
| CN109942091B (en) | Bacterial-algae attached biological fiber bed, preparation method thereof and method for strengthening treatment of pig wastewater for nitrogen and phosphorus removal | |
| CN105820985A (en) | Compound microbial agent for efficiently treating sewage and preparation method of compound microbial agent | |
| CN115287209B (en) | A compound microbial agent and its application in treating swine urine wastewater | |
| CN105060508A (en) | Biological sewage-treatment complex microbial inoculant and preparation method thereof | |
| CN107460148A (en) | A kind of preparation method and application of bacillus subtilis bacterium powder | |
| CN115353211A (en) | Application of bacillus megaterium LZP03 in treatment of pig raising wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150909 |