CN104894033A - Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant - Google Patents

Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant Download PDF

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Publication number
CN104894033A
CN104894033A CN201510355997.7A CN201510355997A CN104894033A CN 104894033 A CN104894033 A CN 104894033A CN 201510355997 A CN201510355997 A CN 201510355997A CN 104894033 A CN104894033 A CN 104894033A
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China
Prior art keywords
sewage
fungus agent
composite fungus
compound microbial
microbial inoculant
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CN201510355997.7A
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Chinese (zh)
Inventor
李克朗
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Hubei Lingzhuo Bioengineering Co Ltd
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Hubei Lingzhuo Bioengineering Co Ltd
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Priority to CN201510355997.7A priority Critical patent/CN104894033A/en
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The invention discloses a compound microbial inoculant for degrading chemical oxygen demand and a preparation method of the compound microbial inoculant. The compound microbial inoculant comprises, by volume, 9-11% of candida tropicalis, 9-11% of candida parapsilosis, 28-32% of bacillus subtilis, 28-32% of bacillus licheniformis and 19-21% of enterococcus faecalis lactobacillus. The preparation method of the compound microbial inoculant includes that the candida tropicalis, the candida parapsilosis, the bacillus subtilis, the bacillus licheniformis and the enterococcus faecalis lactobacillus are respectively inoculated and cultured in sewage culturing media to obtain respective bacterium solutions, and the bacterium solutions are mixed according to the volume ratio to obtain the compound microbial inoculant, wherein the sewage culturing media are abundant in organic matter after being sterilized. The compound microbial inoculant is high in environment adaptability, applicable to microbial enhanced sewage treatment in different areas and various sewage including aquaculture sewage, domestic sewage, landfill leachate and the like which are abundant in organic matter, and capable of removing 95% of the COD in sewage.

Description

Composite fungus agent of a kind of COD that degrades and preparation method thereof
Technical field
The present invention relates to microbiobacterial agent field, specifically the composite fungus agent and preparation method thereof of a kind of COD that degrades.
Background technology
Well-known, current mankind is faced with multiple crisis, such as crisis in food, energy shortage, resource scarcity, ecological degeneration and Population explosion etc.After the mankind enter 21 century, utilize the unlimited Biological resources epoch and a series of new problems produced by running into from utilizing the limited mineral wealth epoch to be transitioned into.Because microorganism cells is that one larger than face amount, biochemical conversion ability strong, can carries out the Living system of quick self-replacation; and they also have the diversity of species, heredity, metabolism and ecotype, make them can play its irreplaceable unique effect in the various crises solving facing mankind.
Industrial pollution source: trade effluent is the important source of pollution in waters, the features such as the amount of having is large, area is wide, complicated component, toxicity are large, should not purify, difficult.Agricultural pollution source: agricultural pollution source comprises excrement of animals, agricultural chemicals, chemical fertilizer etc.China is one of country that soil erosion is the fastest in the world, every chronology soil number of dropouts about 5,000,000,000 tons, the lake of 2/3rds is subject to the harm of eutrophication in various degree, causes algae and other biological distorted proliferation, cause the change of water transparency and dissolved oxygen, thus cause water quality deterioration.Domestic pollution source: the various washing composition that domestic pollution source mainly uses in urban life and sewage, rubbish, ight soil etc., mostly be nontoxic inorganic salts, many containing nitrogen phosphate and sulfur in sanitary sewage, bacterium of curing the disease is many.Existing sewage disposal process by activated sludge process cost is high, and COD (chemical oxygen demand (COD), Chemical Oxygen Demand) clearance is undesirable.
Microbial preparation was existing in water surrounding improvement in recent years applied, but there is a lot of problem, as some microorganisms can not produce enzyme, the small molecules nutritive substance of just simple absorption water body, effectively can not promote the material cycle of water body environment, effectiveness comparison is slow.Bacterial classification is single, and the effect of each microorganism in the link of water body material cycle is different, and show through experiment, composite bacteria effect and processing efficiency are much larger than single culture.
Summary of the invention
The object of the present invention is to provide that a kind of adaptive capacity to environment is strong, COD of sewage is removed and can be reached composite fungus agent of the degraded COD of 95% and preparation method thereof, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
To degrade a composite fungus agent of COD, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 9-11%, Candida parapsilosis 9-11%, subtilis 28-32%, Bacillus licheniformis 28-32%, excrement intestines milk-acid bacteria 19-21%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10 8-5.4 × 10 8individual/ml, Candida parapsilosis 2.3 × 10 8-5.8 × 10 8individual/ml, subtilis 5.5 × 10 8-9.6 × 10 8individual/ml, Bacillus licheniformis 5.0 × 10 8-9.8 × 10 8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10 8-2.6 × 10 8individual/ml.
As the further scheme of the present invention: the composite fungus agent of described degraded COD, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 10%, Candida parapsilosis 10%, subtilis 30%, Bacillus licheniformis 30%, excrement intestines milk-acid bacteria 20%.
The preparation method of the composite fungus agent of described degraded COD, is made up of following steps:
One, cultivate in organic abundant sewage substratum after candida tropicalis, Candida parapsilosis, subtilis, Bacillus licheniformis, excrement intestines milk-acid bacteria being inoculated in sterilizing respectively, obtain respective bacterium liquid; Be 30 DEG C in temperature, shaking speed is that the bacterium number being cultured to bacterium liquid under the condition of 200r/min reaches above-mentioned scope;
Two, above-mentioned bacterium liquid is mixed according to volume ratio, obtain composite fungus agent.
As the further scheme of the present invention: the steam sterilizing condition that step one prepares sewage in the sewage substratum of bacterium liquid is 121 DEG C, 30min.
As the further scheme of the present invention: the working conditions of composite fungus agent requires that sewage pH is 6.0-7.0, temperature 25-35 DEG C.
Strain characteristic selected by the present invention and function as follows:
1) subtilis, can produce amylase and bacillin, again can the organic matter such as degradation of polysaccharide class.
2) Bacillus licheniformis, can produce proteolytic enzyme, bacitracin etc., adapts to all kinds of Sewage Environment ability strong.
3) candida tropicalis, Candida parapsilosis, can produce proteolytic enzyme, and degraded multiple protein class is organic.
4) sausage stuffed mainly with bean starch paste milk-acid bacteria, can produce lactic acid class goods and materials, and the multiple macromole of effective acidication is organic, release small organic molecule matter.
Compared with prior art, the invention has the beneficial effects as follows: composite fungus agent prepared by the present invention adaptive capacity to environment is strong, be applicable to different areas microbial augmentation sewage disposal, be applicable to the sewage that all kinds of organic matters such as aquaculture wastewater, sanitary sewage and percolate class sewage are abundant, COD of sewage is removed can reach 95%.Solve existing sewage disposal process by activated sludge process cost high, the problem that COD clearance is undesirable.Described in specific as follows.
1) microorganism used can the multiple larger molecular organics of efficient degradation.Traditional active sludge contains multiple-microorganism, but superior microorganism Microflora is limited, can not reach the object of efficient degradation, and sludge sump laid down cost is high, and operation maintenance cost is high, to sewage organic matter degradation limited efficacy.
2) microorganism used is based on sewage ancestral home bacterium
Microorganism has certain environmental compatibility as all biologies, traditional microorganism great majority used are Lu Sheng, reach water body and can not adapt to water surrounding, therefore can not breed very soon, form dominant microflora, and ancestral home bacterium is separated from Sewage Environment, to Sewage Environment strong adaptability, artificial input can form dominant microflora rapidly later.
3) microorganism used can also produce microbiotic sample material
Selected microorganism can produce microbiotic sample material as genus bacillus, Bacillus licheniformis, has restraining effect to pathogenic bacterium.
4) microorganism selected by is the mixture of multiple-microorganism, and they have stronger specific aim and combination ability, not mutually exclusive in biologic packing material, mutually coordinates in sewage, and successful improves.Do not need complicated engineering process during use, directly throw in, easy to use.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, aquaculture wastewater process.
1. implement place: Jingshan Area of Hubei plant.
2. water treatment amount: average treatment sewage at 100d/T, process water be animal manure just, higher containing organic matter.
3. using method and technique: utilize composite fungus agent technical finesse sewage.
Concrete treating processes is as follows; Sewage, after anaerobic pond fermentation, through piping from entering Aerobic Pond, at Aerobic Pond aeration, increasing the dissolved oxygen in sewage, making dissolved oxygen reach more than 2ppm.Add artificial bio-membrane's filler at Aerobic Pond, sewage is at the microbial film through there being a large amount of composite fungus agent to be formed, and the nutritive substance in water body is fully absorbed by the composite fungus agent on microbial film, then treated water, flows out through secondary filtration pond.
4. the addition of composite fungus agent: the composite fungus agent on initial stage filler is not also formed before a large amount of adhesion, average water per ton adds composite fungus agent 1 kilogram, composite fungus agent is adsorbed on above filler, after within general about 7 days, having a large amount of composite fungus agent mucous membrane to be formed, just no longer add composite fungus agent, composite fungus agent film is aging come off after, start again to add composite fungus agent.Described composite fungus agent, is made up of the following bacterium liquid according to volume ratio: candida tropicalis 9%, Candida parapsilosis 11%, subtilis 32%, Bacillus licheniformis 28%, excrement intestines milk-acid bacteria 20%.
5. average cost of water treatment is less than 0.3 yuan, and maintenance cost is low.
6. whole engineering of water treatment reduced investment expense is comparatively large, and according to a conventional method, the construction investment processing sewage per ton is at 900 yuan/d, and the construction investment processing sewage per ton declines 100 yuan/ton than traditional method.
7. treatment effect is as follows
During actual motion, composite fungus agent has good place to go effect to pollutent.Composite fungus agent filter tank influent COD mean concns is respectively: 1000.05mg/L, and water outlet water mean concns is respectively: 98.5g/L, and the clearance of dye thing is respectively 90.15%, reaches national grade one discharge standard.
Embodiment 2
In the embodiment of the present invention, town domestic sewage process
1. implement place: sewage farm, Jiangxia.
2. water treatment amount: average treatment sewage is at 200d/T.Process water is town domestic sewage, higher containing organic matter.
3. using method and technique: utilize composite fungus agent technical finesse sewage.
Concrete treating processes is as follows; Sewage, after anaerobic pond fermentation, through piping from entering Aerobic Pond, at Aerobic Pond aeration, increasing the dissolved oxygen in sewage, making dissolved oxygen reach more than 2ppm.Add artificial bio-membrane's filler at Aerobic Pond, sewage is at the microbial film through there being a large amount of composite fungus agent to be formed, and the nutritive substance in water body is fully absorbed by the composite fungus agent on microbial film, then treated water, flows out through filter tank.
4. the addition of composite fungus agent: the composite fungus agent on initial stage filter membrane is not also formed before a large amount of adhesion, average water per ton adds composite fungus agent 1.5 kilograms, composite fungus agent is adsorbed on above filter membrane, a large amount of composite fungus agent within about general 7-8 days, is had to stick after film formed, just no longer add composite fungus agent, composite fungus agent film is aging come off after, start again to add composite fungus agent.Composite fungus agent, is made up of the following bacterium liquid according to volume ratio: candida tropicalis 11%, Candida parapsilosis 9%, subtilis 28%, Bacillus licheniformis 32%, excrement intestines milk-acid bacteria 20%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10 8-5.4 × 10 8individual/ml, Candida parapsilosis 2.3 × 10 8-5.8 × 10 8individual/ml, subtilis 5.5 × 10 8-9.6 × 10 8individual/ml, Bacillus licheniformis 5.0 × 10 8-9.8 × 10 8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10 8-2.6 × 10 8individual/ml.
5. average cost of water treatment is less than 0.3 yuan, and maintenance cost is low.
6. whole engineering of water treatment reduced investment expense is comparatively large, and according to a conventional method, the construction investment processing sewage per ton is at 900 yuan/d, and the construction investment processing sewage per ton declines 110 yuan/ton than traditional method.
7. treatment effect is as follows:
During actual motion, composite fungus agent has good place to go effect to pollutent.Composite fungus agent filter tank influent COD mean concns is respectively: 1400.05mg/L, and water outlet water mean concns is respectively: 95.5g/L, and the clearance of dye thing is respectively 93.18%, reaches national grade one discharge standard.
Embodiment 3
In the embodiment of the present invention, certain food factory effluent process
1. implement place: Wuhan food company sewage farm.
2. water treatment amount: average treatment sewage is at 400d/T.Process water be animal manure just, higher containing organic matter.
3. using method and technique: utilize composite fungus agent technical finesse sewage.
Concrete treating processes is as follows; Sewage, after anaerobic pond fermentation, through piping from entering Aerobic Pond, at Aerobic Pond aeration, increasing the dissolved oxygen in sewage, making dissolved oxygen reach more than 2ppm.Add artificial bio-membrane's filler at Aerobic Pond, sewage is at the microbial film through there being a large amount of composite fungus agent to be formed, and the nutritive substance in water body is fully absorbed by the composite fungus agent on microbial film, then treated water, flows out through filter tank.
4. the addition of composite fungus agent: the composite fungus agent on initial stage filler is not also formed before a large amount of adhesion, average water per ton adds composite fungus agent 1 kilogram, composite fungus agent is adsorbed on above filler, after within general about 7 days, having a large amount of composite fungus agent mucous membrane to be formed, just no longer add composite fungus agent, composite fungus agent film is aging come off after, start again to add composite fungus agent.Composite fungus agent, is made up of the following bacterium liquid according to volume ratio: candida tropicalis 10%, Candida parapsilosis 10%, subtilis 30%, Bacillus licheniformis 30%, excrement intestines milk-acid bacteria 20%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10 8-5.4 × 10 8individual/ml, Candida parapsilosis 2.3 × 10 8-5.8 × 10 8individual/ml, subtilis 5.5 × 10 8-9.6 × 10 8individual/ml, Bacillus licheniformis 5.0 × 10 8-9.8 × 10 8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10 8-2.6 × 10 8individual/ml.
5. average cost of water treatment is less than 0.3 yuan, and maintenance cost is low.
6. whole engineering of water treatment reduced investment expense is comparatively large, and according to a conventional method, the construction investment processing sewage per ton declines 130 yuan/ton than traditional method.
7. treatment effect is as follows:
During actual motion, composite fungus agent has good place to go effect to pollutent.The aerobic influent COD mean concns of composite fungus agent is respectively: 1600.05mg/L, and water outlet water mean concns is respectively: 92.5g/L, and the clearance of pollutent is respectively 94.22%, reaches national grade one discharge standard.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
In addition, be to be understood that, although this specification sheets is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets is only for clarity sake, those skilled in the art should by specification sheets integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.

Claims (5)

1. the composite fungus agent of the COD that degrades, it is characterized in that, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 9-11%, Candida parapsilosis 9-11%, subtilis 28-32%, Bacillus licheniformis 28-32%, excrement intestines milk-acid bacteria 19-21%; And the bacterium number of each bacterium liquid is followed successively by: candida tropicalis 2.1 × 10 8-5.4 × 10 8individual/ml, Candida parapsilosis 2.3 × 10 8-5.8 × 10 8individual/ml, subtilis 5.5 × 10 8-9.6 × 10 8individual/ml, Bacillus licheniformis 5.0 × 10 8-9.8 × 10 8individual/ml, excrement intestines milk-acid bacteria 1.5 × 10 8-2.6 × 10 8individual/ml.
2. the composite fungus agent of degraded COD according to claim 1, it is characterized in that, be made up of the following bacterium liquid according to volume ratio: candida tropicalis 10%, Candida parapsilosis 10%, subtilis 30%, Bacillus licheniformis 30%, excrement intestines milk-acid bacteria 20%.
3. a preparation method for the composite fungus agent of the degraded COD as described in as arbitrary in claim 1-2, is characterized in that, be made up of following steps:
One, cultivate in organic abundant sewage substratum after candida tropicalis, Candida parapsilosis, subtilis, Bacillus licheniformis, excrement intestines milk-acid bacteria being inoculated in sterilizing respectively, obtain respective bacterium liquid; Be 30 DEG C in temperature, shaking speed is that the bacterium number being cultured to bacterium liquid under the condition of 200r/min reaches above-mentioned scope;
Two, above-mentioned bacterium liquid is mixed according to volume ratio, obtain composite fungus agent.
4. the preparation method of the composite fungus agent of degraded COD according to claim 3, it is characterized in that, the steam sterilizing condition that step one prepares sewage in the sewage substratum of bacterium liquid is 121 DEG C, 30min.
5. according to the composite fungus agent of the arbitrary described degraded COD of claim 1-2, it is characterized in that, the working conditions of composite fungus agent requires that sewage pH is 6.0-7.0, temperature 25-35 DEG C.
CN201510355997.7A 2015-06-25 2015-06-25 Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant Pending CN104894033A (en)

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CN105176902A (en) * 2015-09-22 2015-12-23 宁波创蓝环境科技有限公司 Microbial preparation for urban sewage treatment and application thereof
CN106630190A (en) * 2016-12-20 2017-05-10 深圳市禹邦水处理技术有限公司 COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof
CN108342338A (en) * 2018-01-15 2018-07-31 杭州秀川科技有限公司 A kind of processing method of the pharmacy waste water containing antibiotics
CN108441444A (en) * 2018-03-22 2018-08-24 中电建水环境治理技术有限公司 A kind of complex micro organism fungicide administered suitable for black and odorous water
CN109266573A (en) * 2018-09-06 2019-01-25 中海新世纪量子生物科技（深圳）有限责任公司 Landfill leachate and reverse osmosis concentrated liquid processing leavening, microbial inoculum and processing method
CN110438040A (en) * 2019-07-22 2019-11-12 南京润中生物技术有限公司 A kind of lichem bacillus strain and its application
CN112760255A (en) * 2021-01-22 2021-05-07 湖北凌卓生物工程有限公司 Compound microbial inoculum for degrading COD (chemical oxygen demand) and preparation method thereof

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105176902A (en) * 2015-09-22 2015-12-23 宁波创蓝环境科技有限公司 Microbial preparation for urban sewage treatment and application thereof
CN106630190A (en) * 2016-12-20 2017-05-10 深圳市禹邦水处理技术有限公司 COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof
CN106630190B (en) * 2016-12-20 2020-06-16 永旌(湖北)环境技术有限公司 Fungus agent for degrading COD and preparation method thereof
CN108342338A (en) * 2018-01-15 2018-07-31 杭州秀川科技有限公司 A kind of processing method of the pharmacy waste water containing antibiotics
CN108342338B (en) * 2018-01-15 2021-01-19 杭州秀川科技有限公司 Method for treating pharmaceutical wastewater containing antibiotics
CN108441444A (en) * 2018-03-22 2018-08-24 中电建水环境治理技术有限公司 A kind of complex micro organism fungicide administered suitable for black and odorous water
CN108441444B (en) * 2018-03-22 2020-09-25 中电建生态环境集团有限公司 Compound microbial agent suitable for black and odorous water treatment
CN109266573A (en) * 2018-09-06 2019-01-25 中海新世纪量子生物科技（深圳）有限责任公司 Landfill leachate and reverse osmosis concentrated liquid processing leavening, microbial inoculum and processing method
CN110438040A (en) * 2019-07-22 2019-11-12 南京润中生物技术有限公司 A kind of lichem bacillus strain and its application
CN110438040B (en) * 2019-07-22 2022-06-03 南京润中环境科技有限公司 Bacillus licheniformis strain and application thereof
CN112760255A (en) * 2021-01-22 2021-05-07 湖北凌卓生物工程有限公司 Compound microbial inoculum for degrading COD (chemical oxygen demand) and preparation method thereof

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Application publication date: 20150909