CN102583770A - Bamboo charcoal-photosynthetic bacteria integrated municipal sanitary wastewater treating agent - Google Patents
Bamboo charcoal-photosynthetic bacteria integrated municipal sanitary wastewater treating agent Download PDFInfo
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- CN102583770A CN102583770A CN2012100121882A CN201210012188A CN102583770A CN 102583770 A CN102583770 A CN 102583770A CN 2012100121882 A CN2012100121882 A CN 2012100121882A CN 201210012188 A CN201210012188 A CN 201210012188A CN 102583770 A CN102583770 A CN 102583770A
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- bamboo charcoal
- photosynthetic bacterium
- nutrient solution
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- treating agent
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Abstract
The invention discloses a bamboo charcoal-photosynthetic bacteria integrated municipal sanitary wastewater treating agent, and relates to removal and treatment of organic matters and ammonia nitrogen in the municipal sanitary wastewater. The treating agent is obtained by the steps that smashed activated bamboo charcoal is added into concentrated photosynthetic bacteria culture liquid and is adsorbed and filtered. The bamboo charcoal-photosynthetic bacteria integrated municipal sanitary wastewater treating agent has the characteristics of low municipal sanitary wastewater treatment cost, small land occupation, low sludge yield and obvious economic benefit, environment-friendly benefit and social benefit; and by using the treating agent, the COD (chemical oxygen demand) value and the ammonia nitrogen content in the municipal sanitary wastewater can be effectively lowered.
Description
Technical field
The invention belongs to sanitary wastewater biologic treating technique field, be specifically related to the integrated urban domestic wastewater of a kind of bamboo charcoal-photosynthetic bacterium treatment agent.
Background technology
Sanitary wastewater is the general name of the various waste water that produce in people's daily life.Mainly comprise ight soil water, bathing water, washing water and wash-down water etc.Its source also has the waste water of discharges such as various collective units and PW except that family domestic waste water.Its principal feature has: (1) is nitrogenous, phosphorus, sulphur high; (2) contain Mierocrystalline cellulose, starch, carbohydrate, fat, protein, urea etc., under the anaerobic type bacteriological action, be prone to produce malodorous material; (3) contain multiple mikrobe,, be prone to make the people to infect various diseases like bacterium, pathogenic bacteria, virus etc.; 4) because a large amount of uses of washing composition increase its content in waste water, be weakly alkaline, human body is had certain harm.Pollutant component is extremely complicated various in the waste water, and any treatment process all is difficult to reach the purpose that purifies fully, and usually wants several method to form treatment system, just can reach treatment requirement.
Press the difference of degree of treatment, Waste Water Treatment can be divided into one-level processing, second-stage treatment and advanced treatment.
One-level is handled and is only removed the suspended substance in the waste water, is main with physical method, and the waste water after the processing generally can't reach emission standard.
For the second-stage treatment system, it is pre-treatment that one-level is handled.What second-stage treatment was the most frequently used is biological treatment, and it can remove the organism that is colloid and dissolved state in the waste water significantly, makes the waste water allow compliance with emission standards.But also retain algae increment nutrition such as a certain amount of suspended substance, biological indissoluble dissolved organic matter, solvability inorganics and nitrogen phosphorus in the process water of second-stage treatment, and contain virus and bacterium.Thereby the higher emission standard that can not meet the demands, just possibly cause pollution as entering the river that flow is less, dilution capacity is relatively poor after handling, can not directly be used as tap water, process water and phreatic nourishment source; Tertiary treatment is further to remove the pollutent that second-stage treatment fails to remove, like phosphorus, nitrogen and the biological organic pollutant that is difficult to degrade, inorganic pollutant, pathogenic agent etc.The tertiary treatment of waste water is on the basis of second-stage treatment, further adopts chemical method (chemical oxidation, chemical precipitation etc.), physico-chemical processes (absorption, IX, membrane separation technique etc.) to remove a kind of " advanced treatment " method of some specific pollutants.Obviously, the tertiary treatment of waste water is costly, but can make full use of water resources.
((photosynthetic bacteria, PSB) be one type is the energy with light to photosynthetic bacterium, with CO
2Or organism is carbon source, and the hydrosphere mikrobe through photosynthesis is nourished and generated extensively is present in occurring in nature, and is more common in the high waters of spoilage organism matter concentration.Photosynthetic bacterium is an anerobes, itself contains multiple nutrients material and physiologically active substance, has functions such as the photosynthesis of carrying out, fermentation and fixed nitrogen, product hydrogen.Photosynthetic bacterium starts from generation nineteen sixty in Application of Sewage.People such as the Japan positive Thailand of scientist's holt find that high concentrated organic wastewater is the result of the micropopulation ecological succession of Different Nutrition level in natural self-purification process; Photosynthetic bacterium plays crucial effect in this process, and has at first carried out the research of handling organic waste water with the photosynthetic bacterium method.Compare with biological treatment waste water system such as activated sludge process, biomembrance process and the anaerobic process of present widespread use; PSB is under natural lighting and micro-aerobic condition; Can directly carry out high efficiency processing, and before processing, not need waste water is diluted various high concentrated organic wastewaters.Have advantages such as saves energy, water source, equipment and running expense, thalline mud can fully utilize, and as the bait of fish and domestic animal, does not cause secondary pollution.
Bamboo charcoal is high as a kind of porous media material, porosity, specific surface area is big, excellent adsorption, physical and chemical performance are stable, and cheap and easy to get, possesses the performance characteristics that the microbial fixed carrier material should have, and can be used as microbial fixed carrier.About utilizing bamboo charcoal as carrier absorption microbiological treatment waste water, domestic existing report.(water technology, 2009,35 (3): 86-89) reported the treatment effect that utilizes bamboo charcoal absorption EM to improve BAF such as Luo Shujun; (environmental science and technology, 2009,32 (3): 151-154) reported such as Sun Xiang with bamboo charcoal treatment based on immobilized microorganisms phenolic wastewater; (colleges and universities' chemical engineering journal such as Zhou Shan; 2008,22 (5): 889-894) reported, reported (forest-science simultaneously with the research of bamboo charcoal immobilization pseudomonas degradation of phenol; 2009; 45 (6): be carrier 133-138), mikrobes such as nitrifier, denitrifying bacteria are fixed on bamboo charcoal, studied removal and the influence factor of bamboo charcoal immobilized microorganism to ammonia nitrogen with the bamboo charcoal.These methods all are to utilize bamboo charcoal to fix oxygen animalcule or aerobic and mixed bacterial anaerobion; Exist and need aeration in the sewage treatment process, increase cost of sewage disposal; Simultaneously in the mixed bacterial culturing process; Because nutrition and competition for space between each bacterial classification, cause shortcomings such as the bad or comparatively small amt of part growth, thereby influence wastewater treatment efficiency.The fixing photosynthetic bacterial treatment urban domestic wastewater of bamboo charcoal after the employing activation, domestic literature does not appear in the newspapers.
Summary of the invention
Technical problem to be solved by this invention is: to the not enough of the existing treatment technology in urban domestic wastewater and, shortcoming such as investment big high to equipment requirements; Provide a kind of bamboo charcoal-photosynthetic bacterium integrated sanitary wastewater treatment agent; With the treatment and purification urban domestic wastewater, its production technique is simple, cost and energy consumption is low, the investment is little, it is low and treatment effect good to be easy to after large-scale production, the wastewater treatment sludge yield.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopted is: the integrated urban domestic wastewater of a kind of bamboo charcoal-photosynthetic bacterium treatment agent, and it is that the photosynthetic bacterium nutrient solution is concentrated 3-5 doubly; And regulate after pH value is 4.0-5.0, adding is crushed to 5-10 purpose activation bamboo charcoal, at 30-40 ℃; Absorption is 4-5 hour under the 80-100r/min condition; Filter, promptly get, the add-on of activation bamboo charcoal is that every 100ml photosynthetic bacterium nutrient solution adds 15-25g activation bamboo charcoal.
The activation bamboo charcoal market of using among the present invention is on sale.
The photosynthetic bacterium of using among the present invention is a prior art, and culture presevation number is: Rhodopseudomonas palustris (Rhodopseudomonas palustris) CCREM SDMCC 150013, ACCC10650, CGMCC1.2349 or GIM1.167.
The seed liquor of the Rhodopseudomonas palustris of using among the present invention and preparation of fermentation liquid also are prior art.
The working method of Rhodopseudomonas palustris seed liquor is: add NH by every 1000mL water
4Cl 1.0-1.5g, CH
3COONa 2.5-3.5g, MgCl
20.1-0.18g, CaCl
20.1-0.25g, KH
2PO
40.55-0.6g, K
2HPO
40.38-0.52g, and the ratio of yeast extract paste 0.1-0.25g with NH
4Cl, CH
3COONa, MgCl
2, CaCl
2, KH
2PO
4, K
2HPO
4, and yeast extract paste soluble in water; Regulate pH7.5-8.5; 110-115 ℃ of sterilization 25-30 minute, be cooled to 30-40 ℃, insert 1 of Rhodopseudomonas palustris slant strains being preserved in 4 ℃; In intensity of illumination is to cultivate 4-5 days under 2500-40001x, the 32-37 ℃ condition, and getting seed liquor concentration is that viable count content is 8-10 * 10
8The Rhodopseudomonas palustris seed liquor of CFU/mL.
The working method of fermented liquid is: add sodium acetate 3-4g, NH by every 1000mL water
4Cl 2-3g, NaCl 0.1-0.25g, CaCl
20.05-0.08g, KH
2PO
40.5-0.6g, K
2HPO
40.3-0.45g, MgSO
47H
2O 0.2-0.3g, MnSO
42H
2O 0.02-0.035g, FeCl
30.002-0.004g, and the ratio of yeast extract paste 0.1-0.2g with sodium acetate, NH
4Cl, NaCl, CaCl
2, KH
2PO
4, K
2HPO
4, MgSO
47H
2O, MnSO
42H
2O, FeCl
3, and yeast extract paste soluble in water, pH value nature was sterilized 25-30 minute at 110-115 ℃, was cooled to 30-40 ℃.
The preparation of photosynthetic bacterium nutrient solution: inserting above-mentioned viable count content with the inoculum size of 15-30 volume % is 8-10 * 10
8The Rhodopseudomonas palustris seed liquor of CFU/mL is to cultivate 5-7 days under 2500-40001x, the 32-37 ℃ condition in intensity of illumination in above-mentioned fermented liquid, gets final product.
The above-mentioned photosynthetic bacterium nutrient solution that obtains concentrates under vacuum tightness 0.07-0.09MPa, temperature 40-50 ℃, and adopts hydrochloric acid or acetic acid to regulate the pH value.
When adopting product treatment sanitary wastewater of the present invention, every 100g sanitary wastewater need add this product of 0.2-0.5g.
Below in conjunction with concrete data the application of product of the present invention in wastewater treatment is described:
Sanitary wastewater 100kg, its initial COD value is that 418.8mg/L, ammonia-nitrogen content are 26.7mg/L, adds product 0.4kg of the present invention; Stir; Handled 2.5-3 days under room temperature condition and the natural lighting condition, the COD value reduces by 96.79%, and the ammonia nitrogen reduced rate is 84.25%.
Compared with prior art, the present invention has the following advantages:
1, used carrier is a bamboo charcoal, has bigger specific surface area and stronger absorption property, generates microbial film easily.
2, bamboo charcoal is a crude substance, and Rhodopseudomonas palustris can be used as animal feed, does not add any chemical substance in the invention product, and is environmentally friendly, do not cause secondary pollution.
3, can use repeatedly behind the bamboo charcoal absorption photosynthetic bacterium.
4, the employed bacterial classification of the inventive method is an anerobes, handles in the sanitary wastewater process and need not aeration, has reduced cost for wastewater treatment.
5, product processes of the present invention is simple, and the no three wastes produce, and facility investment is little, is easy to large-scale production.
Embodiment
At first prepare the photosynthetic bacterium nutrient solution: add sodium acetate 3g, NH by every 1000mL water
4Cl2g, NaCl0.1g, CaCl
20.05g, KH
2PO
40.5g, K
2HPO
40.3g, MgSO
47H
2O 0.2g, MnSO
42H
2O 0.02g, FeCl
30.002g, and the ratio of yeast extract paste 0.1g with sodium acetate, NH
4Cl, NaCl, CaCl
2, KH
2PO
4, K
2HPO
4, MgSO
47H
2O, MnSO
42H
2O, FeCl
3, and yeast extract paste soluble in water, pH value nature was sterilized 25 minutes at 115 ℃, was cooled to 30 ℃, (seed liquor concentration is 10 * 10 to insert cultured Rhodopseudomonas palustris seed liquor with the inoculum size of 20 volume % respectively again
8CFU/mL), be to cultivate 7 days under 30001x, 35 ℃ of conditions in intensity of illumination, promptly get the photosynthetic bacterium nutrient solution.
Embodiment 1: get the photosynthetic bacterium nutrient solution; Under vacuum tightness 0.085MPa, 40 ℃ of conditions, concentrate 3 times; After regulating pH4.0 with hydrochloric acid, the amount that adds 20g activation bamboo charcoal by every 100ml photosynthetic bacterium nutrient solution adds and is crushed to 10 purpose activation bamboo charcoals, and absorption is 5 hours under 30 ℃, 100r/min condition; Filter, get final product.
Embodiment 2: get the photosynthetic bacterium nutrient solution; Under vacuum tightness 0.07MPa, 50 ℃ of conditions, concentrate 5 times; Regulate pH4.5 with acetic acid, the amount that adds 25g activation bamboo charcoal by every 100ml photosynthetic bacterium nutrient solution adds and to be crushed to 8 purpose activation bamboo charcoals, and absorption is 4.5 hours under 35 ℃, 90r/min condition; Filter, get final product.
Embodiment 3: get the photosynthetic bacterium nutrient solution; Under vacuum tightness 0.08MPa, 45 ℃ of conditions, concentrate 3.5 times; Regulate pH4.8 with hydrochloric acid, the amount that adds 15g activation bamboo charcoal by every 100ml photosynthetic bacterium nutrient solution adds and to be crushed to 5 purpose activation bamboo charcoals, and absorption is 4 hours under 40 ℃, 80r/min condition; Filter, get final product.
Claims (4)
1. the integrated urban domestic wastewater of bamboo charcoal-photosynthetic bacterium treatment agent is characterized in that: be that the photosynthetic bacterium nutrient solution is concentrated 3-5 doubly, and regulate after the pH value is 4.0-5.0; Add and be crushed to 5-10 purpose activation bamboo charcoal; At 30-40 ℃, absorption is 4-5 hour under the 80-100r/min condition, filters; Promptly get, the add-on of activation bamboo charcoal is that every 100ml photosynthetic bacterium nutrient solution adds 15-25g activation bamboo charcoal.
2. the integrated urban domestic wastewater of bamboo charcoal-photosynthetic bacterium as claimed in claim 1 treatment agent, it is characterized in that: the preparation of said photosynthetic bacterium nutrient solution, the bacterial classification of employing are the Rhodopseudomonas palustris seed liquor, viable count content is 8-10 * 10
8CFU/mL, fermented liquid is: every 1000mL water adds sodium acetate 3-4g, NH
4Cl 2-3g, NaCl 0.1-0.25g, CaCl
20.05-0.08g, KH
2PO
40.5-0.6g, K
2HPO
40.3-0.45g, MgSO
47H
2O 0.2-0.3g, MnSO
42H
2O 0.02-0.035g, FeCl
30.002-0.004g, and yeast extract paste 0.1-0.2g, culture condition: inoculum size 15-30 volume %, intensity of illumination is 2500-40001x, temperature 32-37 ℃, incubation time 5-7 days.
3. according to claim 1 or claim 2 the integrated urban domestic wastewater of bamboo charcoal-photosynthetic bacterium treatment agent, it is characterized in that: the concentrated condition of said photosynthetic bacterium nutrient solution is: vacuum tightness 0.07-0.09MPa, temperature 40-50 ℃.
4. according to claim 1 or claim 2 the integrated urban domestic wastewater of bamboo charcoal-photosynthetic bacterium treatment agent, it is characterized in that: the pH value of said photosynthetic bacterium nutrient solution adopts hydrochloric acid or acetic acid to regulate.
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| CN104211170A (en) * | 2014-09-11 | 2014-12-17 | 镇江华域环保设备制造有限公司 | Wastewater treatment agent for wastewater in sugar-making industry |
| CN105923957A (en) * | 2016-07-11 | 2016-09-07 | 许祚员 | Dichlorobenzidine polluted sludge treating agent and preparation method thereof |
| CN105923958A (en) * | 2016-07-11 | 2016-09-07 | 许祚员 | Atrazine-polluted sludge treating agent and preparation method thereof |
| CN107058281A (en) * | 2016-12-20 | 2017-08-18 | 广东韩研活性炭制造有限公司 | A kind of biological activated carbon and preparation method thereof for the COD of sewage that is used to degrading |
| CN112897824A (en) * | 2021-04-26 | 2021-06-04 | 桂林理工大学 | Bamboo charcoal-photosynthetic bacteria-constructed wetland integration sewage treatment system |
| CN114806890A (en) * | 2022-04-26 | 2022-07-29 | 湖南农业大学 | Chili straw lignin degradation microbial inoculum combination and application thereof |
| CN114956352A (en) * | 2022-05-31 | 2022-08-30 | 广西民族大学 | Rhodopseudomonas palustris-nano TiO 2 Composite material and preparation method and application thereof |
| CN116161799A (en) * | 2022-01-07 | 2023-05-26 | 南通聚益成广生物科技有限公司 | Method for treating food waste water by utilizing photosynthetic bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104211170A (en) * | 2014-09-11 | 2014-12-17 | 镇江华域环保设备制造有限公司 | Wastewater treatment agent for wastewater in sugar-making industry |
| CN105923957A (en) * | 2016-07-11 | 2016-09-07 | 许祚员 | Dichlorobenzidine polluted sludge treating agent and preparation method thereof |
| CN105923958A (en) * | 2016-07-11 | 2016-09-07 | 许祚员 | Atrazine-polluted sludge treating agent and preparation method thereof |
| CN107058281A (en) * | 2016-12-20 | 2017-08-18 | 广东韩研活性炭制造有限公司 | A kind of biological activated carbon and preparation method thereof for the COD of sewage that is used to degrading |
| CN112897824A (en) * | 2021-04-26 | 2021-06-04 | 桂林理工大学 | Bamboo charcoal-photosynthetic bacteria-constructed wetland integration sewage treatment system |
| CN116161799A (en) * | 2022-01-07 | 2023-05-26 | 南通聚益成广生物科技有限公司 | Method for treating food waste water by utilizing photosynthetic bacteria |
| CN116161799B (en) * | 2022-01-07 | 2024-04-26 | 南通聚益成广生物科技有限公司 | Method for treating food waste water by utilizing photosynthetic bacteria |
| CN114806890A (en) * | 2022-04-26 | 2022-07-29 | 湖南农业大学 | Chili straw lignin degradation microbial inoculum combination and application thereof |
| CN114956352A (en) * | 2022-05-31 | 2022-08-30 | 广西民族大学 | Rhodopseudomonas palustris-nano TiO 2 Composite material and preparation method and application thereof |
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Application publication date: 20120718 |