CN113215050A - Algae-bacterium symbiotic composite microbial preparation for sewage treatment and preparation method thereof - Google Patents
Algae-bacterium symbiotic composite microbial preparation for sewage treatment and preparation method thereof Download PDFInfo
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- 239000010865 sewage Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 46
- 230000000813 microbial effect Effects 0.000 title claims abstract description 37
- 239000002131 composite material Substances 0.000 title claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 66
- 241000894006 Bacteria Species 0.000 claims abstract description 49
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 41
- 241000195628 Chlorophyta Species 0.000 claims abstract description 39
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 33
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 30
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000009630 liquid culture Methods 0.000 claims description 56
- 239000000243 solution Substances 0.000 claims description 31
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 24
- 238000005286 illumination Methods 0.000 claims description 21
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 12
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 12
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
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- 238000002156 mixing Methods 0.000 claims description 8
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 8
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 241000407778 Bacillus subtilis TO-A Species 0.000 claims description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 241000191043 Rhodobacter sphaeroides Species 0.000 claims description 4
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 4
- 235000019270 ammonium chloride Nutrition 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 229960001031 glucose Drugs 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000004317 sodium nitrate Substances 0.000 claims description 4
- 235000010344 sodium nitrate Nutrition 0.000 claims description 4
- 235000010288 sodium nitrite Nutrition 0.000 claims description 4
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 claims description 4
- 239000004324 sodium propionate Substances 0.000 claims description 4
- 235000010334 sodium propionate Nutrition 0.000 claims description 4
- 229960003212 sodium propionate Drugs 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 3
- 240000009108 Chlorella vulgaris Species 0.000 claims description 2
- 235000007089 Chlorella vulgaris Nutrition 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims 2
- 238000004065 wastewater treatment Methods 0.000 claims 2
- 244000249201 Scenedesmus obliquus Species 0.000 claims 1
- 235000007122 Scenedesmus obliquus Nutrition 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 16
- 238000012258 culturing Methods 0.000 abstract description 2
- 230000001546 nitrifying effect Effects 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 9
- 239000011574 phosphorus Substances 0.000 description 9
- 229910052698 phosphorus Inorganic materials 0.000 description 9
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 7
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- 208000035985 Body Odor Diseases 0.000 description 1
- 206010059410 Faecaluria Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010040904 Skin odour abnormal Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 208000011140 intestinal infectious disease Diseases 0.000 description 1
- 244000000074 intestinal pathogen Species 0.000 description 1
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- 238000005272 metallurgy Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
Abstract
A method for preparing phycobiont composite microbial preparation for sewage treatment comprises respectively culturing photosynthetic bacteria, Bacillus subtilis and nitrifying bacteria in a photobioreactor containing corresponding culture medium; secondly, inoculating the green algae into a photobioreactor of a green algae culture medium for culture; when in use, the obtained photosynthetic bacteria, bacillus subtilis and nitrobacteria culture solution are uniformly mixed according to equal proportion, and then the microalgae culture solution is mixed and uniformly stirred according to the volume ratio of 50:3, so that the composite microbial preparation for treating sewage containing the photosynthetic bacteria, the bacillus subtilis, the nitrobacteria and the green algae is obtained. The composite microbial preparation prepared by the method is mainly based on the principle that the microbial preparation compounded by photosynthetic bacteria, bacillus subtilis, nitrobacteria and green algae is used for treating polluted and odorous water sources such as rivers, reservoirs and the like, so that secondary pollution of a water area is avoided, and treated sewage can reach the standard of a normal water body.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to a composite microbial preparation, in particular to a phycobiont composite microbial preparation for sewage treatment and a preparation method thereof.
[ background of the invention ]
The normal water body is odorless and smellless, and the polluted water body is often accompanied with strong odor. Since the 70 s, the water pollution is becoming more serious, the eutrophication degree is deepened, and the condition that the water is smelly is very common, which not only influences the sensory quality of the drinking water, but also seriously damages the environmental quality. The causes of water body pollution are various, and the main sources thereof are as follows: (1) industrial wastewater: industrial waste water is a major cause of pollution worldwide. Wastewater can be produced in each link of the industrial production process. The industrial wastewater with larger influence mainly comes from metallurgy, electroplating, papermaking, printing and dyeing and tanning enterprises. (2) Domestic sewage: refers to the washing wastewater and the fecaluria sewage of people's daily life. The sewage from medical units is a special domestic sewage, and the main harm is to cause intestinal infectious diseases. (3) Agricultural sewage: mainly contains nitrogenous, phosphorus and potassium fertilizers, pesticides, organic substances of excrement and urine and human and livestock intestinal pathogens. (4) And others: the solid waste produced in the industrial production process contains a large amount of inorganic and organic matters which are easily dissolved in water and is polluted by rainwater. Along with the development of economy, the requirements of people on life quality and environment quality are higher and higher, and the call for solving the problem of water body odor is stronger and stronger. Therefore, there is a need for an environment-friendly material without secondary pollution to solve the problem of water pollution.
[ summary of the invention ]
In order to solve the problems, the invention provides an algae-bacteria symbiotic composite microbial preparation for sewage treatment, which utilizes a microbial preparation compounded by photosynthetic bacteria, bacillus subtilis, nitrobacteria and green algae to treat polluted and odorous river and reservoir polluted water sources, so that secondary pollution of a water area is avoided, and treated sewage can reach the national ground surface water quality standard.
The invention is realized by the following technical scheme, and provides a preparation method of a phycobiont composite microbial preparation for sewage treatment, which comprises the following steps:
s1, inoculating photosynthetic bacteria purified and cultured in a culture dish into a photobioreactor with a 100L photosynthetic bacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 1500-5000 lux and temperature of 25-35 ℃ to obtain a liquid culture solution containing the photosynthetic bacteria; inoculating bacillus subtilis to a photobioreactor which is provided with 100L of bacillus subtilis culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 500-1000 lux and temperature of 25-37 ℃ to obtain liquid culture solution containing the bacillus subtilis; inoculating nitrobacteria into a photobioreactor which is provided with 100L of nitrobacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.0-8.0, light shielding and temperature of 20-30 ℃ to obtain liquid culture solution containing nitrobacteria; in the step, photosynthetic bacteria, bacillus subtilis and nitrobacteria can all utilize light energy and organic matters to provide nutrient sources for metabolic propagation of the photosynthetic bacteria, and meanwhile, in the step, the pH, the illumination intensity and the temperature can all influence the growth of the three bacteria, if the pH, the illumination intensity and the temperature are not in corresponding ranges, the growth of the three bacteria can be inhibited, so that the biomass is too low and even the cells die, and if the culture time is too short, the biomass is too low and too long, the production efficiency can be influenced;
s2, inoculating the green algae purified and cultured in a culture dish into a photobioreactor of a 100L green algae culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.5-8.5, illumination intensity of 1500-4000 lux and temperature of 22-32 ℃ in cooperation with aeration to obtain a green algae liquid culture solution; in the step, the growth of the green algae is influenced by pH, illumination intensity and temperature, if the growth of the green algae is not in a corresponding range, the growth of the green algae is inhibited, the biomass is too low and even the cells die, and if the culture time is too short, the biomass is too low and too long, the production efficiency is influenced;
s3, adding the photosynthetic bacteria-containing liquid culture solution, the bacillus subtilis liquid culture solution and the nitrobacteria liquid culture solution obtained in S1 into a transparent container in equal proportion to obtain a mixed solution, separately storing the obtained mixed solution and the green algae liquid culture solution obtained in S2 under the conditions of temperature of 25-30 ℃ and illumination intensity of 500-1000 lux, and mixing and uniformly stirring the mixed solution and the green algae liquid culture solution according to the volume ratio of 50:3 when the composite microbial preparation is used, so as to obtain the composite microbial preparation for treating the sewage containing the photosynthetic bacteria and the green algae; in the step, the mixing according to the volume ratio of 50:3 can improve the defects of insufficient sewage treatment efficiency and incomplete treatment (removing ammonia nitrogen and phosphorus by bacteria and removing heavy metals by algae) of a single strain or a single algae strain, thereby improving the sewage treatment efficiency.
Specifically, the photosynthetic bacteria, bacillus subtilis and nitrobacteria in the S1 are subjected to purification culture in a culture dish before inoculation, and the Scenedesmus chlororaphis and chlorella in the S2 are also subjected to purification culture in the culture dish before inoculation.
In particular, the photosynthetic bacteria in the S1 are a mixture of rhodopseudomonas palustris and rhodobacter sphaeroides.
In particular, the green algae is a mixture of Scenedesmus sp and Chlorella sp, in the invention, the cell wall of the green algae contains 24-74% of polysaccharide, 2-16% of protein and 1-24% of uronic acid, which can provide amino, amido, carbonyl and hydroxyl functional groups to combine with metal ions, and the cell membrane is a semi-permeable membrane with high selectivity, and these structural characteristics determine the adsorption possibility and adsorption selectivity of the green algae to the metal ions.
Specifically, the culture medium for the photosynthetic bacteria in S1 comprises the following components: 2.8-3.2 g/L of sodium acetate, 0.2-0.4 g/L of sodium propionate, 0.21-0.41 g/L of magnesium sulfate, 0.2-0.4 g/L of dipotassium hydrogen phosphate, 1.5-1.7 g/L of potassium dihydrogen phosphate, 0.8-1.2 g/L of sodium chloride and 1.4-1.8 g/L of ammonium chloride, sterilizing the prepared liquid culture medium at the temperature of 121 ℃ for 15-30 minutes, and cooling for later use; the bacillus subtilis culture medium comprises the following components: 3.0-4.0 g/L of anhydrous glucose, 0.6-0.9 g/L of peptone, 0.3-0.4 g/L of monopotassium phosphate and 0.21-0.32 g/L of calcium carbonate, sterilizing the liquid culture medium at the temperature of 121 ℃ for 15-30 minutes after the liquid culture medium is prepared, and cooling the liquid culture medium for later use; the nitrobacteria culture medium comprises the following components: 0.8-1.1 g/L of sodium nitrite, 0.02-0.04 g/L of magnesium sulfate, 7.0-8.0 g/L of calcium chloride, 0.7-0.8 g/L of monopotassium phosphate and 0.15-0.35 g/L of sodium dihydrogen phosphate, sterilizing the liquid culture medium at the temperature of 121 ℃ for 15-30 minutes after the liquid culture medium is prepared, and cooling the liquid culture medium for later use; in the invention, the selection of the culture medium corresponding to each kind of strains can provide elements required for the growth of photosynthetic bacteria, bacillus subtilis and nitrobacteria, if the selection range of each component is less than or more than the selection range of each component, the growth of the photosynthetic bacteria, the bacillus subtilis and the nitrobacteria is not facilitated, and meanwhile, the control of temperature and the like can avoid the pollution of the culture medium by mixed bacteria, thereby providing an environment which is beneficial to the growth of the photosynthetic bacteria, the bacillus subtilis and the nitrobacteria.
In particular, the composition of the chlorella culture medium in S2 is as follows: 0.3-0.6 g/L of sodium nitrate, 0.33-0.53 g/L of monopotassium phosphate, 0.15-0.35 g/L of magnesium sulfate, 0.02-0.04 g/L of ferrous sulfate, 0.01-0.03 g/L of calcium chloride and 0.02-0.04 g/L of EDTA, wherein the green alga liquid culture medium is sterilized for 15-30 minutes at the temperature of 121 ℃ after being prepared, and is cooled for later use; in the invention, the green algae culture medium can provide elements required for the growth of green algae, if the selection range of the green algae culture medium is lower than or higher than the selection range of each component, the growth of the green algae is not facilitated, and meanwhile, the control of temperature and the like can avoid the pollution of the culture medium by mixed bacteria, thereby providing an environment which is beneficial to the growth of the green algae.
The invention also provides the phycobiont composite microbial preparation for sewage treatment, which is prepared by the preparation method of the phycobiont composite microbial preparation for sewage treatment.
Particularly, when the composite microbial preparation is used for sewage treatment, the volume ratio of the composite microbial preparation to sewage is 1: (1000-2000) the method is applied to sewage treatment.
The invention provides a compound microbial preparation prepared from green algae, photosynthetic bacteria, bacillus subtilis and nitrobacteria and a preparation method thereof. In the sewage treatment process, the chlorella and the photosynthetic bacteria are compounded by utilizing the symbiotic concept of the phycomycetes, the efficiency of the obtained microbial preparation for sewage treatment is obviously improved compared with that of a single photosynthetic bacteria strain for sewage treatment, and the standard of 0-level (tasteless) or 1-level (barely feeling the odor) odor intensity can be reached after the treatment is finished.
[ detailed description ] embodiments
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
Example (b):
the algae and bacteria symbiotic composite microbial preparation provided by the invention is adopted for sewage treatment, and the implementation is carried out according to the following method:
firstly, preparing a phycobiont composite microbial preparation for sewage treatment, and culturing photosynthetic bacteria (rhodopseudomonas palustris and rhodobacter sphaeroides), bacillus subtilis and nitrobacteria and green algae (scenedesmus and chlorella culture medium) according to the following steps:
1. inoculating photosynthetic bacteria purified and cultured in a culture dish into a photobioreactor with 100L of photosynthetic bacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 1500-5000 lux and temperature of 25-35 ℃ to obtain liquid culture solution containing the photosynthetic bacteria; inoculating bacillus subtilis to a photobioreactor which is provided with 100L of bacillus subtilis culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 500-1000 lux and temperature of 25-37 ℃ to obtain liquid culture solution containing the bacillus subtilis; inoculating the nitrobacteria into a photobioreactor which is provided with 100L of nitrobacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.0-8.0, light shielding and temperature of 20-30 ℃ to obtain liquid culture solution containing the nitrobacteria.
Wherein the photosynthetic bacteria culture medium comprises the following components: 2.8-3.2 g/L of sodium acetate, 0.2-0.4 g/L of sodium propionate, 0.21-0.41 g/L of magnesium sulfate, 0.2-0.4 g/L of dipotassium hydrogen phosphate, 1.5-1.7 g/L of potassium dihydrogen phosphate, 0.8-1.2 g/L of sodium chloride and 1.4-1.8 g/L of ammonium chloride, sterilizing the prepared liquid culture medium at the temperature of 121 ℃ for 15-30 minutes, and cooling for later use; the bacillus subtilis culture medium comprises the following components: 3.0-4.0 g/L of anhydrous glucose, 0.6-0.9 g/L of peptone, 0.3-0.4 g/L of monopotassium phosphate and 0.21-0.32 g/L of calcium carbonate, sterilizing the liquid culture medium at the temperature of 121 ℃ for 15-30 minutes after the liquid culture medium is prepared, and cooling the liquid culture medium for later use; the nitrobacteria culture medium comprises the following components: 0.8-1.1 g/L of sodium nitrite, 0.02-0.04 g/L of magnesium sulfate, 7.0-8.0 g/L of calcium chloride, 0.7-0.8 g/L of monopotassium phosphate and 0.15-0.35 g/L of sodium dihydrogen phosphate, wherein the liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being prepared, and is cooled for later use.
2. Inoculating Scenedesmus and chlorella which are purified and cultured in a culture dish into a 100L photobioreactor filled with a green algae cell activation culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.5-8.5, illumination intensity of 1500-4000 lux and temperature of 22-32 ℃ and aeration matching, thereby obtaining a liquid culture solution of green algae.
Wherein, the green algae cell activation culture medium comprises the following components: 0.3-0.6 g/L of sodium nitrate, 0.33-0.53 g/L of monopotassium phosphate, 0.15-0.35 g/L of magnesium sulfate, 0.02-0.04 g/L of ferrous sulfate, 0.01-0.03 g/L of calcium chloride and 0.02-0.04 g/L of EDTA, wherein the green alga liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being prepared, and is cooled for later use.
3. And mixing the liquid culture solution containing the activated photosynthetic bacteria, the bacillus subtilis and the nitrobacteria in equal proportion after the culture is finished and separately storing the obtained liquid culture solution containing the activated green algae under the conditions of temperature of 25-30 ℃ and illumination intensity of 500-1000 lux, and mixing and uniformly stirring the liquid culture solution according to the volume ratio of 50:3 when the liquid culture solution is used, thereby obtaining the composite microbial preparation for treating sewage containing the photosynthetic bacteria, the bacillus subtilis, the nitrobacteria and the green algae.
4. The method comprises the following steps of (1) mixing an algae bacterium symbiotic composite microbial preparation for sewage treatment and pond reservoir sewage in a volume of 1: 1000-2000, adding the composite microbial preparation into sewage at the initial ammonia nitrogen content of 0.772mg/L, the total phosphorus content of 0.521mg/L and the total nitrogen content of 3.458mg/L, wherein the treatment temperature is 25-35 ℃, the treatment time is 20 days, and the illumination is normal sunlight. The ammonia nitrogen content of the treated lake water is 0.091mg/L, the total phosphorus content is 0.236mg/L, and the total nitrogen content is 1.031mg/L, which can reach the standard of odor intensity of grade 0 (tasteless) or grade 1 (barely feeling odor).
Comparative example 1
The following steps of treating sewage by using photosynthetic bacteria (rhodopseudomonas palustris, rhodobacter sphaeroides), bacillus subtilis and nitrobacteria are specifically carried out according to the following method:
1. inoculating photosynthetic bacteria purified and cultured in a culture dish into a photobioreactor with 100L of photosynthetic bacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 1500-5000 lux and temperature of 25-35 ℃ to obtain liquid culture solution containing the photosynthetic bacteria; inoculating bacillus subtilis to a photobioreactor which is provided with 100L of bacillus subtilis culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 500-1000 lux and temperature of 25-37 ℃ to obtain liquid culture solution containing the bacillus subtilis; inoculating the nitrobacteria into a photobioreactor which is provided with 100L of nitrobacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.0-8.0, light shielding and temperature of 20-30 ℃ to obtain liquid culture solution containing the nitrobacteria.
Wherein the photosynthetic bacteria culture medium comprises the following components: 2.8-3.2 g/L of sodium acetate, 0.2-0.4 g/L of sodium propionate, 0.21-0.41 g/L of magnesium sulfate, 0.2-0.4 g/L of dipotassium hydrogen phosphate, 1.5-1.7 g/L of potassium dihydrogen phosphate, 0.8-1.2 g/L of sodium chloride and 1.4-1.8 g/L of ammonium chloride, wherein the liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being matched, and is cooled for later use; the bacillus subtilis culture medium comprises the following components: 3.0-4.0 g/L of anhydrous glucose, 0.6-0.9 g/L of peptone, 0.3-0.4 g/L of monopotassium phosphate and 0.21-0.32 g/L of calcium carbonate, sterilizing the liquid culture medium at the temperature of 121 ℃ for 15-30 minutes after the liquid culture medium is prepared, and cooling the liquid culture medium for later use; the nitrobacteria culture medium comprises the following components: 0.8-1.1 g/L of sodium nitrite, 0.02-0.04 g/L of magnesium sulfate, 7.0-8.0 g/L of calcium chloride, 0.7-0.8 g/L of monopotassium phosphate and 0.15-0.35 g/L of sodium dihydrogen phosphate, wherein the liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being prepared, and is cooled for later use.
2. Mixing the obtained composite microbial preparation and pond reservoir sewage in a volume ratio of 1: 1000-2000, adding the composite microbial preparation into sewage, wherein the ammonia nitrogen content of the sewage is 0.772mg/L, the total phosphorus content of the sewage is 0.521mg/L, the total nitrogen content of the sewage is 3.458mg/L, the treatment temperature is 25-35 ℃, the treatment time is 20 days, the illumination is normal sunlight, the ammonia nitrogen content of the treated lake water is 0.488mg/L, the total phosphorus content of the sewage is 0.34mg/L, and the total nitrogen content of the sewage is 1.641mg/L, and the standard of class 2 (weak odor and capable of distinguishing the properties) odor intensity is achieved.
Comparative example 2
The sewage is treated by adopting green algae (scenedesmus and chlorella) according to the following method:
1. inoculating the green algae (Scenedesmus sp and chlorella vulgaris) which are purified and cultured in a culture dish into a 100ml photobioreactor filled with a green algae cell activation culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.5-8.5, illumination intensity of 1500-4000 lux and temperature of 22-32 ℃ and aeration matching, thereby obtaining a liquid culture solution of the green algae.
Wherein, the green algae cell activation culture medium comprises the following components: 0.3-0.6 g/L of sodium nitrate, 0.33-0.53 g/L of monopotassium phosphate, 0.15-0.35 g/L of magnesium sulfate, 0.02-0.04 g/L of ferrous sulfate, 0.01-0.03 g/L of calcium chloride and 0.02-0.04 g/L of EDTA, wherein the green alga liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being prepared, and is cooled for later use.
2, mixing the microbial preparation and pond reservoir sewage in a volume ratio of 1: 10000-20000, the composite microbial preparation is added into sewage, the initial ammonia nitrogen content of the sewage is 0.772mg/L, the total phosphorus content of the sewage is 0.521mg/L, the total nitrogen content of the sewage is 3.458mg/L, the treatment temperature is 25-35 ℃, the treatment time is 20 days, the illumination is normal sunlight, the ammonia nitrogen content of the treated lake water is 0.581mg/L, the total phosphorus content of the treated lake water is 0.468mg/L, and the total nitrogen content of the treated lake water is 2.322mg/L, and the standard of odor intensity of grade 3 (which can easily feel odor) or grade 4 (which can strongly feel odor) can be generally achieved, and no obvious effect is achieved.
As can be seen from the above examples and comparative examples, the sewage treatment effect of the phycobiont composite microbial preparation provided by the invention is better, the removal rate of ammonia nitrogen, total phosphorus and total nitrogen in the sewage is higher, the treated sewage can reach the standard of 0-grade (odorless) or 1-grade (barely feeling odor) odor intensity, the treatment requirement is met, and the treatment effects of comparative example 1 and comparative example 2 obviously do not reach the treatment requirement.
Claims (8)
1. A preparation method of a phycobiont composite microbial preparation for sewage treatment is characterized by comprising the following steps:
s1, inoculating photosynthetic bacteria into a photobioreactor with a 100L photosynthetic bacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 1500-5000 lux and temperature of 25-35 ℃ to obtain a liquid culture solution containing the photosynthetic bacteria; inoculating bacillus subtilis to a photobioreactor which is provided with 100L of bacillus subtilis culture medium, and performing static culture for 7-10 days under the culture conditions of pH7.0-8.0, illumination intensity of 500-1000 lux and temperature of 25-37 ℃ to obtain liquid culture solution containing the bacillus subtilis; inoculating nitrobacteria into a photobioreactor which is provided with 100L of nitrobacteria culture medium, and performing static culture for 7-10 days under the culture conditions of pH 7.0-8.0, light shielding and temperature of 20-30 ℃ to obtain liquid culture solution containing nitrobacteria;
s2, inoculating green algae into a photobioreactor of a 100L green algae culture medium, and performing static culture for 7-10 days under the conditions of pH 7.5-8.5, illumination intensity of 1500-4000 lux and temperature of 22-32 ℃ in combination with aeration culture to obtain a green algae liquid culture solution;
s3, adding the photosynthetic bacteria-containing liquid culture solution, the bacillus subtilis liquid culture solution and the nitrobacteria liquid culture solution obtained in S1 into a transparent container in equal proportion to obtain a mixed solution, separately storing the obtained mixed solution and the green alga liquid culture solution obtained in S2 at the temperature of 25-30 ℃ and under the illumination intensity of 500-1000 lux, and mixing and stirring the obtained mixed solution and the green alga liquid culture solution obtained in S2 according to the volume ratio of 50:3 when the composite microbial preparation is used, so as to obtain the composite microbial preparation for sewage treatment containing photosynthetic bacteria and green alga.
2. The method of claim 1, wherein the photosynthetic bacteria, bacillus subtilis and nitrobacteria are subjected to purification culture in a culture dish before inoculation in S1, and the green algae are subjected to purification culture in the culture dish before inoculation in S2.
3. The method for preparing the phycobiont composite microbial preparation for wastewater treatment as claimed in claim 1, wherein said photosynthetic bacteria in S1 is a mixture of rhodopseudomonas palustris and rhodobacter sphaeroides.
4. The method of claim 1, wherein the green algae is a mixture of Scenedesmus obliquus and Chlorella vulgaris.
5. The method for preparing the phycobiont composite microbial preparation for sewage treatment as claimed in claim 1, wherein said culture medium of photosynthetic bacteria in S1 comprises the following components: 2.8-3.2 g/L of sodium acetate, 0.2-0.4 g/L of sodium propionate, 0.21-0.41 g/L of magnesium sulfate, 0.2-0.4 g/L of dipotassium hydrogen phosphate, 1.5-1.7 g/L of potassium dihydrogen phosphate, 0.8-1.2 g/L of sodium chloride and 1.4-1.8 g/L of ammonium chloride, wherein the liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being matched, and is cooled for later use; the bacillus subtilis culture medium comprises the following components: 3.0-4.0 g/L of anhydrous glucose, 0.6-0.9 g/L of peptone, 0.3-0.4 g/L of monopotassium phosphate and 0.21-0.32 g/L of calcium carbonate, sterilizing the liquid culture medium at the temperature of 121 ℃ for 15-30 minutes after the liquid culture medium is prepared, and cooling the liquid culture medium for later use; the nitrobacteria culture medium comprises the following components: 0.8-1.1 g/L of sodium nitrite, 0.02-0.04 g/L of magnesium sulfate, 7.0-8.0 g/L of calcium chloride, 0.7-0.8 g/L of monopotassium phosphate and 0.15-0.35 g/L of sodium dihydrogen phosphate, wherein the liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being prepared, and is cooled for later use.
6. The method for preparing the phycobiont composite microbial preparation for wastewater treatment as claimed in claim 1, wherein the composition of the chlorella culture medium in S2 is as follows: 0.3-0.6 g/L of sodium nitrate, 0.33-0.53 g/L of monopotassium phosphate, 0.15-0.35 g/L of magnesium sulfate, 0.02-0.04 g/L of ferrous sulfate, 0.01-0.03 g/L of calcium chloride and 0.02-0.04 g/L of EDTA, wherein the green alga liquid culture medium is sterilized at the temperature of 121 ℃ for 15-30 minutes after being prepared, and is cooled for later use.
7. A phycobiont composite microbial preparation for sewage treatment prepared by the method for preparing a phycobiont composite microbial preparation for sewage treatment according to any one of claims 1 to 6.
8. The phycobiont composite microorganism preparation for sewage treatment according to claim 7, wherein the composite microorganism preparation is mixed with sewage in a volume ratio of 1: (1000-2000) the method is applied to sewage treatment.
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