CN109370954A - It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method - Google Patents
It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method Download PDFInfo
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Abstract
Invention describes a kind of composite bacteria agents for sewage treatment, preparation method and application method, composite bacteria agent includes the component of following volume parts: 20 parts~50 parts of nitrobacteria, 20 parts~50 parts of denitrifying bacteria, 10 parts~30 parts of algae, the preparation method of the composite bacteria agent includes the following steps: prepared by nitrobacteria fermentation liquid, the preparation of denitrifying bacteria fermentation liquid, algae solution preparation, composite bacteria agent preparation, the application method of the composite bacteria agent is directly to be thrown to composite bacteria agent to administer in water body, without carrying out activation or other steps, injected volume is 1~2L/m3.The composite bacteria agent for sewage treatment that the present invention introduces, the higher position reason activity to ammonia nitrogen can be kept in low dissolved oxygen water body, while can also cut down the phosphorus in water body, reach the high-efficient purification effect to water body, preparation method and application method are simple, are suitable for large-scale popularization.
Description
Technical field
The present invention relates to technical field of water environment treatment, and in particular to a kind of composite bacteria agent for sewage treatment,
Preparation method and application method.
Background technique
Important component of the water resource as natural resources, is not only basic resource for the survival of mankind, even more society
The economy resource that can develop, while the essential ecological resources of Eco-Environment System cycle operation are still supported, it is the mankind
One of itself and nature, the most important basic substance of social sustainable development.With the increase of population, rapid development of economy
With the raising of living standards of the people, China's total amount of sewage discharge is continuously increased.Since sanitary sewage, industrial wastewater and rainwater are carried secretly
Polluter cause black and odorous water have become water environment in China pollution severely afflicated area.
Due to historical reasons, China's black and odorous water storage is huge, and the water pollution origin cause of formation is more, ammonia nitrogen and total in these water bodys
Phosphorus content is higher, and water body dissolved oxygen is low, and excessive nitrogen, phosphorus cause water eutrophication, endanger aquatile, brings to human health
It threatens.Currently, the method for water body denitrification dephosphorization mainly has physical method, chemical method and bioanalysis.Physical method and chemical method there is
Operating cost is high, and improvement is not thorough, secondary pollution problems, in practical applications by more limitation.Bioanalysis denitrogenation dephosphorizing
It is pollution-free with high efficiency, low cost, the advantages that thorough is administered, international endorsement is obtained, becomes main administering method in recent years.It is raw
Object dephosphorization reaches degradation water body using frustule own growth breeding demand active absorption water systems'phosphorus to be transformed into Self substances
The effect of middle phosphorus, biological removal of nitrogen convert nitrogen for ammonia nitrogen, organic nitrogen etc. using the nitrification of microorganism, denitrification, thus
Remove water body in ammonia nitrogen, biological phosphate-eliminating denitrogenate be it is a kind of cleaning, the method that low energy consumption, without secondary pollution.
In practical applications, nitrifying process need to consume a large amount of oxygen, research shows that when water body dissolved oxygen is lower than 0.5mg/L, nitre
It is turned into and is finished full cut-off and stops, thus acts on degradation water body ammonia nitrogen using biological nitration and be subject to certain restrictions in the application；In addition, ammonia nitrogen
Pollution and phosphorus pollution usually coexist in a system, need simultaneously to handle two kinds of pollutants.
Summary of the invention
In order to solve above-mentioned deficiency in the prior art, invention describes a kind of compound biological bacteriums for sewage treatment
Agent can keep to the higher position of ammonia nitrogen reason activity in low dissolved oxygen water body, while can also cut down the phosphorus in water body, reach pair
The high-efficient purification effect of water body, the present invention also describe the preparation method and application method of the composite bacteria agent.
For realize foregoing invention purpose, the technical scheme adopted by the invention is as follows:
A kind of composite bacteria agent for sewage treatment, the component including following volume parts: 20 parts of nitrobacteria~
50 parts, 20 parts~50 parts of denitrifying bacteria, 10 parts~30 parts of algae.
Preferably, denitrifying bacteria includes bacillus subtilis, bacillus pumilus and bacillus licheniformis, withered grass
The deposit number of bacillus is ATCC 35021, and the deposit number of bacillus pumilus is ATCC 19646, lichens gemma bar
The deposit number of bacterium is ATCC 53757.
Preferably, algae includes one or more of chlorella, scenedesmus, silk algae, stonewort.
Preferably, the cell density of nitrobacteria is 3.58~6.25 × 10 in composite bacteria agent10CFU/mL,
The cell density of denitrifying bacteria is 7.25~9.98 × 109CFU/mL, alga cells concentration are 2.55~4.25 × 107A/
mL。
A kind of preparation method of the composite bacteria agent for sewage treatment, includes the following steps:
(1) nitrobacteria fermentation liquid is prepared, the bacterial strain of commercially available nitrobacteria is subjected to scribing line culture on solid medium,
Obtain activation bacterium；The activation bacterium of picking individual colonies, which is seeded in fluid nutrient medium, to be expanded culture, and kind of a daughter bacteria is obtained；By seed
Bacterium is seeded in fermentor and is cultivated, 37 DEG C of fermentation temperature, fermentation time 48h, obtain cell density be 7.34~9.59 ×
1010The nitrobacteria fermentation liquid of CFU/mL；
(2) denitrifying bacteria fermentation liquid is prepared, by bacillus subtilis ATCC 35021, bacillus pumilus ATCC
The bacterial strain of 19646 and bacillus licheniformis ATCC 53757 carries out scribing line culture on solid medium, obtains activation bacterium；It selects
The activation bacterium of single colonie, which is seeded in fluid nutrient medium, to be expanded culture, and kind of a daughter bacteria is obtained；Kind of a daughter bacteria is seeded to fermentor
In cultivated, 37 DEG C of fermentation temperature, fermentation time 48h, obtain cell density be 1.67~2.59 × 1010The anti-nitre of CFU/mL
Change ferment product, wherein the cell density of bacillus subtilis is 7.17~8.60 × 109CFU/mL, bacillus pumilus
Cell density is 4.37~6.45 × 109CFU/mL, the cell density of bacillus licheniformis are 6.58~7.39 × 109CFU/mL；
(3) algae solution is prepared, commercially available green alga is accessed in BG-11 culture medium, 25 DEG C of temperature, intensity of illumination 6000Lux, training
Support time 7d, periodicity of illumination are as follows: light application time 14h, interlunation 10h, it is 1.47~2.36 × 10 that cell density, which is made,9A/
The algae solution of mL；
(4) composite bacteria agent is prepared, by the resulting nitrobacteria fermentation liquid of step (1), step (2) resulting denitrification
Ferment product, step (3) resulting algae solution are mixed and made into composite bacteria agent after measuring according to volume parts.
Preferably, the solid culture based formulas in step (1) includes following weight percentage components: sodium nitrite
0.05%~0.15%, magnesium sulfate 0.002%~0.004%, manganese sulfate 0.0005%~0.0015%, dipotassium hydrogen phosphate
0.05%~0.1%, natrium carbonicum calcinatum 0.05%~0.15%, sodium dihydrogen phosphate 0.02%~0.03%, agar 1%~
1.5%, surplus is water；The pH value of solid medium in step (1) is 7.0~7.5, and in 121 DEG C of sterilizing 30min.
Liquid Culture based formulas in step (1) includes following weight percentage components: sodium nitrite 0.05%~
0.15%, magnesium sulfate 0.002%~0.004%, manganese sulfate 0.0005%~0.0015%, dipotassium hydrogen phosphate 0.05%~
0.1%, natrium carbonicum calcinatum 0.05%~0.15%, sodium dihydrogen phosphate 0.02%~0.03%, surplus is water；In step (1)
The pH value of fluid nutrient medium is 7.0~7.5, and in 121 DEG C of sterilizing 30min.
Preferably, the solid culture based formulas in step (2) includes following weight percentage components: sodium chloride
0.05%~0.15%, yeast powder 0.02%~0.03%, peptone 0.05%~0.15%, agar 1%~1.5%, surplus
For water；The pH value of solid medium in step (2) is 7.0~7.5, and in 121 DEG C of sterilizing 30min.
Liquid Culture based formulas in step (2) includes following weight percentage components: sodium chloride 0.05%~
0.15%, yeast powder 0.02%~0.03%, peptone 0.05%~0.15%, surplus is water；Liquid Culture in step (2)
The pH value of base is 7.0~7.5, and in 121 DEG C of sterilizing 30min.
Preferably, the BG-11 culture medium in step (3) includes following weight percentage components: sodium nitrate
0.01%~0.02%, dipotassium hydrogen phosphate 0.0003%~0.0005%, seven water manganese sulfates 0.005%~0.01%, seven water chlorine
Change calcium 0.0003%~0.0004%, sodium carbonate 0.0001%~0.0003%, citric acid 0.00005%~0.00007%, lemon
Lemon acid iron ammonium 0.00005%~0.00007%, EDTA 0.000005%~0.000015%, microelement A5 0.08%~
0.12%, ampicillin 0.003%~0.008%, surplus is water.
Preferably, microelement A5 includes following weight percentage components: boric acid 0.02%~0.03%, seven water
Zinc sulfate 0.002%~0.003%, sodium molybdate 0.003%~0.005%, cupric sulfate pentahydrate 0.0007%~0.0009%, six
Water cobalt nitrate 0.0004%~0.0006%, surplus are water.
A kind of application method of the composite bacteria agent for sewage treatment are as follows: composite bacteria agent is directly thrown to and is controlled
It manages in water body, injected volume is 1~2L/m3。
Compared with prior art, beneficial effects of the present invention are as follows:
(1) function and effect are strong, and the nitrobacteria and anti-nitre for being used for degradation of ammonia nitrogen are contained in composite bacteria agent of the invention
Dephosphorization can be reached and denitrogenate double effects, while the photosynthetic work of algae also containing the algae for degrade phosphorus and nitrogen by changing bacterium
It can be utilized by nitrobacteria with the oxygen of release, even if so that nitrobacteria in the water body of low dissolved oxygen, can also have higher
Activity, increase the proliferative speed of nitrobacteria, improve the speed and ability of ammonia nitrogen in nitrobacteria degradation water body；
(2) application method is simple, composite bacteria agent of the invention, and composite bacteria agent need to be only sprinkled into water i.e. by when use
Can, without activation, additional physics oxygen increasing equipment is not needed yet, that there are secondary pollution problems in chemical agent, is thrown yet
Stable flora can be formed faster after entering water body, and harmful microbe is inhibited to breed, to reach the mesh to purify water
's.
Specific embodiment
Combined with specific embodiments below, the present invention will be further elaborated.
Embodiment one
This embodiment describes a kind of composite bacteria agent for sewage treatment, the component including following volume parts:
40 parts of nitrobacteria, 40 parts of denitrifying bacteria, 20 parts of silk algae, wherein nitrobacteria includes bacillus subtilis, short and small gemma bar
Bacterium and bacillus licheniformis.
In the composite bacteria agent for sewage treatment of the present embodiment, nitrobacteria cell density be 5.34 ×
1010CFU/mL, denitrifying bacteria cell density are 9.34 × 109CFU/mL, silk frustule concentration are 3.18 × 107A/mL.
Embodiment two
This embodiment describes a kind of composite bacteria agent for sewage treatment, the component including following volume parts:
50 parts of nitrobacteria, 40 parts of denitrifying bacteria, 10 parts of chlorella, wherein nitrobacteria includes bacillus subtilis, short and small gemma
Bacillus and bacillus licheniformis.
In the composite bacteria agent for sewage treatment of the present embodiment, nitrobacteria cell density be 6.25 ×
1010CFU/mL, denitrifying bacteria cell density are 9.34 × 109CFU/mL, chlorella cells concentration are 2.55 × 107A/mL.
Embodiment three
This embodiment describes a kind of composite bacteria agent for sewage treatment, the component including following volume parts:
20 parts of nitrobacteria, 50 parts of denitrifying bacteria, 30 parts of scenedesmus, wherein nitrobacteria includes bacillus subtilis, short and small gemma bar
Bacterium and bacillus licheniformis.
In the composite bacteria agent for sewage treatment of the present embodiment, nitrobacteria cell density be 3.58 ×
1010CFU/mL, denitrifying bacteria cell density are 9.98 × 109CFU/mL, scenedesmus cell concentration are 4.25 × 107A/mL.
Example IV
This embodiment describes a kind of composite bacteria agent for sewage treatment, the component including following volume parts:
50 parts of nitrobacteria, 30 parts of denitrifying bacteria, 20 parts of chlorella, wherein nitrobacteria includes bacillus subtilis, short and small gemma
Bacillus and bacillus licheniformis.
In the composite bacteria agent for sewage treatment of the present embodiment, nitrobacteria cell density be 6.25 ×
1010CFU/mL, denitrifying bacteria cell density are 8.29 × 109CFU/mL, chlorella cells concentration are 3.18 × 107A/mL.
Embodiment five
This embodiment describes a kind of preparation methods of composite bacteria agent for sewage treatment, include the following steps:
(1) nitrobacteria fermentation liquid is prepared, the bacterial strain of commercially available nitrobacteria is subjected to scribing line culture on solid medium,
Obtain activation bacterium；The activation bacterium of picking individual colonies, which is seeded in 500mL fluid nutrient medium, to be expanded culture, and kind of a daughter bacteria is obtained；
Kind of a daughter bacteria is seeded in 5L fermentor and is cultivated, 37 DEG C of fermentation temperature, fermentation time 48h, obtaining cell density is 7.34
~9.59 × 1010The nitrobacteria fermentation liquid of CFU/mL；
(2) denitrifying bacteria fermentation liquid is prepared, by bacillus subtilis ATCC 35021, bacillus pumilus ATCC
The bacterial strain of 19646 and bacillus licheniformis ATCC 53757 carries out scribing line culture on solid medium, obtains activation bacterium；It selects
The activation bacterium of single colonie, which is seeded in 500mL fluid nutrient medium, to be expanded culture, and kind of a daughter bacteria is obtained；Kind of a daughter bacteria is seeded to 5L
It is cultivated in fermentor, 37 DEG C of fermentation temperature, fermentation time 48h, obtaining cell density is 1.67~2.59 × 1010CFU/mL
Denitrifying bacteria fermentation liquid, wherein the cell density of bacillus subtilis be 7.17~8.60 × 109CFU/mL, short and small bud
The cell density of spore bacillus is 4.37~6.45 × 109CFU/mL and the cell density of bacillus licheniformis be 6.58~7.39 ×
109CFU/mL；
(3) algae solution is prepared, commercially available green alga is accessed in 5L BG-11 culture medium, 25 DEG C of temperature, intensity of illumination
6000Lux, periodicity of illumination 14h:10h (illumination: dark), incubation time 7d, it is 1.47~2.36 × 10 that cell density, which is made,9
A/mL algae solution；
(4) composite bacteria agent is prepared
By the resulting nitrobacteria fermentation liquid of step (1), the resulting denitrifying bacteria fermentation liquid of step (2), step (3) institute
The algae solution obtained is mixed and made into the composite bacteria agent of embodiment one after measuring according to the volume ratio of 40:40:20；
By the resulting nitrobacteria fermentation liquid of step (1), the resulting denitrifying bacteria fermentation liquid of step (2), step (3) institute
The algae solution obtained is mixed and made into the composite bacteria agent of embodiment two after measuring according to the volume ratio of 50:40:10；
By the resulting nitrobacteria fermentation liquid of step (1), the resulting denitrifying bacteria fermentation liquid of step (2), step (3) institute
The algae solution obtained is mixed and made into the composite bacteria agent of embodiment three after measuring according to the volume ratio of 20:50:30；
By the resulting nitrobacteria fermentation liquid of step (1), the resulting denitrifying bacteria fermentation liquid of step (2), step (3) institute
The algae solution obtained is mixed and made into the composite bacteria agent of example IV after measuring according to the volume ratio of 50:30:20.
In the present embodiment, the solid culture based formulas of step (1) includes following weight percentage components: sodium nitrite
0.1%, magnesium sulfate 0.003%, manganese sulfate 0.001%, dipotassium hydrogen phosphate 0.075%, natrium carbonicum calcinatum 0.1%, biphosphate
Sodium 0.025%, agar 1.5%, the pH value of the solid medium in step (1) are 7.0, and in 121 DEG C of sterilizing 30min；
The Liquid Culture based formulas of step (1) includes following weight percentage components: sodium nitrite 0.1%, magnesium sulfate
0.003%, manganese sulfate 0.001%, dipotassium hydrogen phosphate 0.075%, natrium carbonicum calcinatum 0.1%, sodium dihydrogen phosphate 0.025%, step
Suddenly the pH value of the fluid nutrient medium in (1) is 7.0, and in 121 DEG C of sterilizing 30min；
Solid culture based formulas in step (2) includes following weight percentage components: sodium chloride 0.1%, yeast powder
0.025%, peptone 0.1%, agar 1%1.5%, surplus is water；The pH value of solid medium in step (2) is 7.0, and
In 121 DEG C of sterilizing 30min.
Liquid Culture based formulas in step (2) includes following weight percentage components: sodium chloride 0.1%, yeast powder
0.025%, peptone 0.1%, surplus is water；The pH value of fluid nutrient medium in step (2) is 7.0, and sterilizes at 121 DEG C
30min。
BG-11 culture medium in step (3) includes following weight percentage components: sodium nitrate 0.015%, phosphoric acid hydrogen two
Potassium 0.0004%, seven water manganese sulfates 0.0075%, seven water calcium chloride 0.00036%, sodium carbonate 0.0002%, citric acid
0.00006%, ferric citrate 0.00006%, EDTA 0.00001%, microelement A5 0.1%, ampicillin
0.005%, surplus is water；
Wherein microelement A5 includes following weight percentage components: boric acid 0.0268%, white vitriol
0.00222%, sodium molybdate 0.0039%, cupric sulfate pentahydrate 0.00079%, cobalt nitrate hexahydrate 0.00049%, surplus is water.
Embodiment five
This embodiment describes a kind of application methods of composite bacteria agent for sewage treatment, by embodiment one, in fact
It applies the composite bacteria agent that example two, embodiment three, example IV any one embodiment are enumerated and is directly thrown to and administer in water body,
Without being activated, without being aerated, injected volume be 1~2L/m3。
Experimental example one
The composite bacteria agent prepared according to the preparation method of example IV is used in sewage treatment, according to embodiment five
Application method add composite bacteria agent into polluted-water, the dissolved oxygen of polluted-water used in this experimental example is 0.48mg/L.
This experimental example designs five groups of experiments altogether, wherein one group is designed as blank control group, in addition four groups of experiments add respectively
The bacteria agent of embodiment one, the bacteria agent of embodiment two, the bacteria agent of embodiment three and example IV bacteria agent,
It is denoted as experimental group one, experimental group two, experimental group three and experimental group four respectively, every group of bacteria agent dosage is 1.4L/m3,
Each experimental group and control group are sampled respectively adding in the different time sections in composite bacteria agent one month, use National Standard Method
The content of water body ammonia nitrogen and total phosphorus is detected, testing result is as shown in table 1:
The content balance of the water body ammonia nitrogen and total phosphorus of each experimental group of 1 different times of table and control group
Data in contrast table 1 it can be seen that, add bacteria agent in the polluted-water that water body dissolved oxygen only has 0.48mg/L
After one month, microbial bacterial agent of the invention is obvious to the degradation effect of nitrogen phosphorus, after especially adding bacteria agent 10d, water body
Self-purification capacity obviously die down, and microbial bacterial agent of the invention also shows outstanding treatment effect, when adding 30d, adds
The ammonia nitrogen removal frank of the bacteria agent of embodiment one has reached 96.8%, and the removal rate of total phosphorus has reached 88.5%.
Composite bacteria agent component of the invention and content are different, and additive amount is different, go to ammonia nitrogen in sewage and total phosphorus
Removing solid capacity is different, needs to select different component and content and different additive amounts according to practical water quality situation.
The above is merely preferred embodiments of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within principle.
Claims (10)
1. a kind of composite bacteria agent for sewage treatment, which is characterized in that the component including following volume parts: nitrification is thin
20 parts~50 parts of bacterium, 20 parts~50 parts of denitrifying bacteria, 10 parts~30 parts of algae.
2. the composite bacteria agent according to claim 1 for sewage treatment, which is characterized in that the denitrifying bacteria
Including bacillus subtilis, bacillus pumilus and bacillus licheniformis.
3. the composite bacteria agent according to claim 1 for sewage treatment, which is characterized in that the algae includes small
One or more of ball algae, scenedesmus, silk algae, stonewort.
4. the composite bacteria agent according to claim 1 for sewage treatment, which is characterized in that the compound biological bacterium
In agent, the cell density of the nitrobacteria is 3.58~6.25 × 1010The cell density of CFU/mL, the denitrifying bacteria is
7.25~9.98 × 109CFU/mL, the alga cells concentration are 2.55~4.25 × 107A/mL.
5. the preparation method of any composite bacteria agent for sewage treatment of Claims 1 to 4, which is characterized in that
Include the following steps:
(1) nitrobacteria fermentation liquid is prepared, the bacterial strain of commercially available nitrobacteria is subjected to scribing line culture on solid medium, is obtained
Activate bacterium；The activation bacterium of picking individual colonies, which is seeded in fluid nutrient medium, to be expanded culture, and kind of a daughter bacteria is obtained；Kind of a daughter bacteria is connect
Kind cultivated into fermentor, 37 DEG C of fermentation temperature, fermentation time 48h, obtain cell density be 7.34~9.59 ×
1010The nitrobacteria fermentation liquid of CFU/mL；
(2) denitrifying bacteria fermentation liquid is prepared, the bacterial strain of bacillus subtilis, bacillus pumilus and bacillus licheniformis is existed
Scribing line culture is carried out on solid medium, obtains activation bacterium；The activation bacterium of picking individual colonies, which is seeded in fluid nutrient medium, to carry out
Expand culture, obtains kind of a daughter bacteria；Kind of a daughter bacteria is seeded in fermentor and is cultivated, 37 DEG C of fermentation temperature, fermentation time 48h,
Obtaining cell density is 1.67~2.59 × 1010The denitrifying bacteria fermentation liquid of CFU/mL, wherein the bacillus subtilis
Cell density is 7.17~8.60 × 109CFU/mL, the cell density of the bacillus pumilus is 4.37~6.45 ×
109CFU/mL, the cell density of the bacillus licheniformis are 6.58~7.39 × 109CFU/mL；
(3) algae solution is prepared, commercially available green alga is accessed in BG-11 culture medium, 25 DEG C of temperature, intensity of illumination 6000Lux, when culture
Between 7d, periodicity of illumination are as follows: light application time 14h, interlunation 10h, be made cell density be 1.47~2.36 × 109A/mL's
Algae solution；
(4) composite bacteria agent is prepared, by the resulting nitrobacteria fermentation liquid of step (1), step (2) resulting denitrifying bacteria
Fermentation liquid, step (3) resulting algae solution are mixed and made into composite bacteria agent after measuring according to volume parts.
6. the preparation method of the composite bacteria agent according to claim 5 for sewage treatment, which is characterized in that described
The solid culture based formulas in step (1) includes following weight percentage components: sodium nitrite 0.05%~0.15%,
Magnesium sulfate 0.002%~0.004%, manganese sulfate 0.0005%~0.0015%, dipotassium hydrogen phosphate 0.05%~0.1% are anhydrous
Sodium carbonate 0.05%~0.15%, sodium dihydrogen phosphate 0.02%~0.03%, agar 1%~1.5%, surplus are water；The step
Suddenly the pH value of the solid medium in (1) is 7.0~7.5, and in 121 DEG C of sterilizing 30min；
The Liquid Culture based formulas in the step (1) includes following weight percentage components: sodium nitrite 0.05%
~0.15%, magnesium sulfate 0.002%~0.004%, manganese sulfate 0.0005%~0.0015%, dipotassium hydrogen phosphate 0.05%~
0.1%, natrium carbonicum calcinatum 0.05%~0.15%, sodium dihydrogen phosphate 0.02%~0.03%, surplus is water；The step (1)
In the pH value of the fluid nutrient medium be 7.0~7.5, and in 121 DEG C of sterilizing 30min.
7. the preparation method of the composite bacteria agent according to claim 5 for sewage treatment, which is characterized in that described
The solid culture based formulas in step (2) includes following weight percentage components: sodium chloride 0.05%~0.15%, ferment
Female powder 0.02%~0.03%, peptone 0.05%~0.15%, agar 1%~1.5%, surplus are water；In the step (2)
The pH value of the solid medium be 7.0~7.5, and in 121 DEG C of sterilizing 30min；
The Liquid Culture based formulas in the step (2) includes following weight percentage components: sodium chloride 0.05%~
0.15%, yeast powder 0.02%~0.03%, peptone 0.05%~0.15%, surplus is water；It is described in the step (2)
The pH value of fluid nutrient medium is 7.0~7.5, and in 121 DEG C of sterilizing 30min.
8. the preparation method of the composite bacteria agent according to claim 5 for sewage treatment, which is characterized in that described
BG-11 culture medium in step (3) includes following weight percentage components: sodium nitrate 0.01%~0.02%, phosphoric acid hydrogen two
Potassium 0.0003%~0.0005%, seven water manganese sulfates 0.005%~0.01%, seven water calcium chloride 0.0003%~0.0004%,
Sodium carbonate 0.0001%~0.0003%, citric acid 0.00005%~0.00007%, ferric citrate 0.00005%~
0.00007%, EDTA 0.000005%~0.000015%, microelement A50.08%~0.12%, ampicillin
0.003%~0.008%, surplus is water.
9. the preparation method of the composite bacteria agent according to claim 8 for sewage treatment, which is characterized in that described
Microelement A5 includes following weight percentage components: boric acid 0.02%~0.03%, and white vitriol 0.002%~
0.003%, sodium molybdate 0.003%~0.005%, cupric sulfate pentahydrate 0.0007%~0.0009%, cobalt nitrate hexahydrate
0.0004%~0.0006%, surplus is water.
10. the application method of any composite bacteria agent for sewage treatment of claim 1~9, which is characterized in that
The composite bacteria agent is directly thrown to and is administered in water body, injected volume is 1~2L/m3。
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