CN103589672A - Method for preparing effective microorganism water quality purification agent - Google Patents
Method for preparing effective microorganism water quality purification agent Download PDFInfo
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- CN103589672A CN103589672A CN201310571106.2A CN201310571106A CN103589672A CN 103589672 A CN103589672 A CN 103589672A CN 201310571106 A CN201310571106 A CN 201310571106A CN 103589672 A CN103589672 A CN 103589672A
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Abstract
The invention discloses an effective microorganism water quality purification agent used for sewage treatment and water quality purification. The agent is a liquid mixture formed by mixing of following microorganisms: nitrogen-fixing bacteria, nitrobacteria, phosphorus-solubilizing bacteria, yeast, lactic acid bacteria, photosynthetic bacteria, actinomyces and growth bacteria. The effective microorganism number of the effective microorganism water quality purification agent reaches 4*10<5> microorganisms/ml. The agent is used for sewage treatment to enable sewage to reach emission indexes, the water quality is improved, effects of cooxidation, ammoniation, denitrification, phosphorus dissolving, sulfuration, nitrogen fixation and the like are achieved, and the good ecological water environment is kept. The agent is free of secondary pollution, simple in technology, low in investment, low in cost and remarkable in water quality purification effect.
Description
Technical field
The microorganism agent system of the present invention relates to, particularly a kind of waste water control, the preparation method of effective bacterium water quality cleansing agent of purification of water quality.
Background technology
Along with scientific and technical development; environment protection more and more comes into one's own; the water purification agent that China is used at present mainly contains inorganic and organic two large classes; inorganic and organic polymer water purification agent be widely used in various water conditioning time, the secondary pollution that has also caused insecurity in use procedure and caused to environment.Therefore, develop a kind of safety non-toxic, effect is remarkable, and the novel water quality cleansing agent of non-secondary pollution has very important realistic meaning for the mankind's healthy and safe and environment protection.In the later stage eighties 20th century, along with the development of biotechnology, the 3rd class water purification agent---microbial water-purifying agent arises at the historic moment.
On the general study of China about microbial water-purifying agent, see, function singleness, only removes suspended substance or color in water, but can not effectively remove metal ion or organic pollutant in water.Therefore people have and make good use of oxygen and anaerobic technique carries out the processing of organic pollutant, reach water body discharge index.But having caused technical process complicated, long processing period, has high input, high in cost of production series of problems.
At present, on market, microbial preparation product is more, and the probiotics of its main component has the microorganism species of photosynthetic bacterium, budding fungus, spore tooth bacillus class, actinomycetes, milk-acid bacteria, nitrobacteria, denitrifying bacterium etc.It is few that yet general microbial preparation on the market generally only contains probiotics kind, the effect of each probiotics is different, and the available substrate kind of single microorganism is limited, the pollutent of water body thoroughly can not be degraded, thereby different microorganisms need to be coordinated to collaborative playing a role.
Summary of the invention
The object of this invention is to provide a kind of non-secondary pollution, technique is simple, drop into little, cost is low, the significant microorganism water quality cleansing agent of effects of purification quality
To achieve these goals, technical scheme of the present invention is: a kind of preparation method of effective bacterium water quality cleansing agent is provided, comprising following steps:
(1) preparation of substratum
By di(2-ethylhexyl)phosphate argon 0.3g, ammonia chloride 0.6g, sodium bicarbonate 5g, sodium sulphite 3g, ammonium sulfate iron 0.3g, after the above composition of glucose 3g is mixed, through 200～300 ℃ of high-temperature sterilizations 20～25 minutes, obtains substratum;
(2) inoculation
By vinelandii, yeast, milk-acid bacteria, Nitromonas, phosphorus-solubilizing bacteria, photosynthetic bacterium, actinomycetes, growth bacterium is inoculated in step (1) substratum, and cultivate respectively 8 hours every day successively under anaerobism, illumination and three kinds of conditions of room temperature, cultivates after 12 days, obtain sorrel symbiosis microorganism species, vinelandii wherein, yeast, milk-acid bacteria, Nitromonas, phosphorus-solubilizing bacteria, photosynthetic bacterium, actinomycetes, the interpolation weight ratio of growth bacterium is 1:1:1:1:1:1:1:1;
Sorrel symbiosis microorganism species analysis of physical and chemical property is as follows:
(3) sewage is removed impurity
The fecal sewage of pig farm discharge is processed to solid-liquid separating machine via ight soil and remove the impurity in sewage, obtain the sewage of removing impurity;
(4) prepare effective bacterium water quality cleansing agent
Sorrel symbiosis microorganism species is added in the sewage that step 3 removes impurity, carry out at normal temperatures oxygenation ferment 6～7 days, reaction makes its sewage be golden yellow, can obtain effective bacterium water quality cleansing agent.
The component of water quality cleansing agent of the present invention and effect thereof:
Vinelandii: fixedly nature nitrogen molecule is nitrogenous source, manufacture fertile part of algae.
Nitromonas: transforming poisonous ammonia is nitrate nitrogen.
Phosphorus-solubilizing bacteria: untie insoluble phosphoric acid, particularly ferrophosphorus calcium.
Yeast: manufacture VITAMIN, auxogen, decomposing organic matter increases disease-resistant body.
Milk-acid bacteria: secretion organic acid, increases disease resistance.
Photosynthetic bacterium: manufacture glucose secretion Lei Gu trailing plants Portugal element, the toxic substances such as removal of hydrogen sulfide and ammonia produce.
Actinomycetes: secrete for a long time quantitative antibiotics, suppress disease.
Growth bacterium: secrete for a long time quantitative growth.
The present invention is according to the degradation characteristic of bacterial classification, be compounded with the floras such as vinelandii, Nitromonas, phosphorus-solubilizing bacteria, yeast, milk-acid bacteria, photosynthetic bacterium, actinomycetes, growth bacterium, can in water body environment, improve survival rate, by its nutritive property, immunological characteristic and three aspects of improving the ecological environment, sewage is administered, reached the index of discharge.The present invention is through the improvement of the breeding wastewater for sieve Ox Mountain pig farm, and the water quality after improvement is far superior to emission standard, and technique is simple, and treatment cycle is short, and cost is low.
Embodiment
Embodiment 1 processes the sewage of 90t
1, the preparation of substratum: di(2-ethylhexyl)phosphate argon 0.3g, ammonia chloride 0.6g, sodium bicarbonate 5g, sodium sulphite 3g, ammonium sulfate iron 0.3g, glucose 3g, after above composition is mixed through 200 ℃ of autoclavings 20 minutes;
2, inoculation: by the vinelandii of 25 grams, 25 grams of yeast, 25 grams of milk-acid bacterias, 25 grams of Nitromonass, 25 grams of phosphorus-solubilizing bacterias, 25 grams of photosynthetic bacteriums, 25 grams of actinomycetes, 25 grams of growth bacterium are all inoculated in the substratum that step 1 prepares, cultivate respectively 8 hours every day successively under anaerobism, illumination and three kinds of conditions of room temperature, cultivate after 12 days, can grow sorrel symbiosis microorganism species, obtain the sorrel symbiosis microorganism species of 300 grams;
3, whole fecal sewages of pig farm discharge are processed to solid-liquid separating machine via ight soil and remove the impurity in sewage, must remove the sewage of impurity;
4, the sorrel symbiosis microorganism species of 300 grams step 2 being prepared adds in the sewage after step 3 is processed, and carries out at normal temperatures oxygenation ferment 6 days, and reaction makes its water be golden yellow, can obtain effective bacterium water quality cleansing agent of 0.6t.
Embodiment 2 processes the sewage of 150t
1, the preparation of substratum: di(2-ethylhexyl)phosphate argon 0.3g, ammonia chloride 0.6g, sodium bicarbonate 5g, sodium sulphite 3g, ammonium sulfate iron 0.3g, glucose 3g, after above composition is mixed through 250 ℃ of autoclavings 25 minutes;
2, inoculation: by 42 vinelandii, 42 grams of yeast, 42 grams of milk-acid bacterias, 42 grams of Nitromonass, 42 grams of phosphorus-solubilizing bacterias, 42 grams of photosynthetic bacteriums, 42 grams of actinomycetes, 42 grams of growth bacterium are all inoculated in the substratum that step 1 prepares, cultivate respectively 8 hours every day successively under anaerobism, illumination and three kinds of conditions of room temperature, cultivate after 12 days, can grow sorrel symbiosis microorganism species, obtain the sorrel symbiosis microorganism species of 500 grams;
3, whole fecal sewages of pig farm discharge are processed to solid-liquid separating machine via ight soil and remove the impurity in sewage, must remove the sewage of impurity;
4, the sorrel symbiosis microorganism species of 500 grams step 2 being prepared adds in the sewage after step 3 is processed, and carries out at normal temperatures oxygenation ferment 6 days, and reaction makes its water be golden yellow, can obtain effective bacterium water quality cleansing agent of 1t.
Embodiment 3 processes the sewage of 300t
1, the preparation of substratum: di(2-ethylhexyl)phosphate argon 0.3g, ammonia chloride 0.6g, sodium bicarbonate 5g, sodium sulphite 3g, ammonium sulfate iron 0.3g, glucose 3g, after above composition is mixed through 300 ℃ of autoclavings 20 minutes;
2, inoculation: by the vinelandii of 83 grams, 83 grams of yeast, 83 grams of milk-acid bacterias, 83 grams of Nitromonass, 83 grams of phosphorus-solubilizing bacterias, 83 grams of photosynthetic bacteriums, 83 grams of actinomycetes, 83 grams of growth bacterium are all inoculated in the substratum that step 1 prepares, cultivate respectively 8 hours every day successively under anaerobism, illumination and three kinds of conditions of room temperature, cultivate after 12 days, can grow sorrel symbiosis microorganism species, obtain the sorrel symbiosis microorganism species of 1000 grams;
3, whole fecal sewages of pig farm discharge are processed to solid-liquid separating machine via ight soil and remove the impurity in sewage, must remove the sewage of impurity;
4, the sorrel symbiosis microorganism species of 1000 grams step 2 being prepared adds in the sewage after step 3 is processed, and carries out at normal temperatures oxygenation ferment 6 days, and reaction makes its water be golden yellow, can obtain the water quality cleansing agent of 2t.
Apply effective bacterium water quality cleansing agent of the present invention and process the sewage on Hainan sieve Ox Mountain limited-liability company pig farm:
Hainan sieve Ox Mountain limited-liability company pig farm: this in pig farm year livestock on hand 4000 bulls medium-sized pig farm; in feeding process of live pig, produce and contain in a large number animal manure high density just and rush hurdle sewage; for protection water source and ecotope; promote the Sustainable development of Intensive Farm of Pig Raising; holder our company of special commission administers sewage, to reach the standard of discharge.Concrete operation step is as follows:
1, sewage, through grid, is removed impurity, and precipitation.
2, a certain amount of porous masonry, charcoal, bamboo charcoal, zeolite etc. are placed in the effective bacterium purification of water quality of the present invention agent solution and are soaked after 2～4 days, be thrown into sewage and be positioned over water outlet.Purification has the Shi，Ru riverbed, waters of certain flow rate there is no mud, should throw the special cement block that can not wash away into as microbial film.
3, get in the first groove 0.6t sewage that the effective bacterium water quality cleansing agent of 300ml the present invention adds sewage reaction tank, at normal temperature and oxygenating machine, beat under the condition of oxygen, ferment 7 days;
4. add the second reactive tank to process in the fermentation sewage of 7 days in reaction tank the first groove, the rear sewage of processing is transmitted back to the second reactive tank via the 3rd reactive tank and carries out secondary response again.Oxygenating machine is housed in reaction tank to be beaten oxygen and provides oxygen condition for reaction.(the circular treatment reactive system of reaction tank is three grades of purification tanks, the sewage entering is first store into the first groove, by effective bacterium water quality cleansing agent, decomposed, organic substance decomposing Cheng Shui and carbonic acid gas in second groove sewage, the 3rd groove sent treated water back to top treatment trough again, so circulation, can increase beneficial microorganism density, improves the resistance to oxidation of water.）
5, get effective bacterium water quality cleansing agent 50L of the present invention and spray sewage surface, during sewage purification, water can keep flow state better.
6, via the reacted sewage of reaction tank via solid-liquid separating machine, the sewage transport after processing, to back pool, be can be used for to agricultural irrigation and swinery and rinses.
Organic contamination in sewage is the nutritive substance of many aerobic bacterias normally, as long as dissolved oxygen in water is enough, these bacteriums will add inferior utilization and produce oxidisability decomposition reaction.
Following table 1 is for adopting the effective bacterium water quality cleansing agent of the present invention to process sewage quality and the emission standard after the sewage on Hainan sieve Ox Mountain limited-liability company pig farm, and table 2 is the contrast of water quality parameter before and after sewage disposal.
Table 1 sewage quality and emission standard
| Project | Ammonia nitrogen | Suspended substance | Chemical oxygen demand (COD) | PH | Five-day BOD |
| Sewage quality | 360.5mg/L | 850.1mg/L | 5586mg/L | 9.3 | 2670mg/L |
| Pollution exhaust criteria | ≤80 | ≤200 | ≤400 | 6﹣9 | ≤150 |
Water quality parameter contrast before and after table 2 sewage disposal
| Sample title | Sewage quality before processing | Sewage quality after processing |
| Ammonia nitrogen | 360.1mg/L | 48.0mg/L |
| Suspended substance | 850.1mg/L | 68mg/L |
| Chemical oxygen demand (COD) | 5586mg/L | 106mg/L |
| PH | 9.3 | 7.43 |
| Five-day BOD | 2670mg/L | 35mg/L |
Conclusion: sewage reaches national discharge index after treatment.
Above disclosed is only preferred embodiment of the present invention, certainly can not limit with this interest field of the present invention, and the equivalent variations of therefore doing according to the claims in the present invention, still belongs to the scope that the present invention is contained.
Claims (1)
1. a preparation method for effective bacterium water quality cleansing agent, is characterized in that comprising the steps:
(1) preparation of substratum
By di(2-ethylhexyl)phosphate argon 0.3g, ammonia chloride 0.6g, sodium bicarbonate 5g, sodium sulphite 3g, ammonium sulfate iron 0.3g, after the above composition of glucose 3g is mixed, through 200～300 ℃ of high-temperature sterilizations 20～25 minutes, obtains substratum;
(2) inoculation
By vinelandii, yeast, milk-acid bacteria, Nitromonas, phosphorus-solubilizing bacteria, photosynthetic bacterium, actinomycetes, growth bacterium is inoculated in step (1) substratum, and cultivate respectively 8 hours every day successively under anaerobism, illumination and three kinds of conditions of room temperature, cultivates after 12 days, obtain sorrel symbiosis microorganism species, vinelandii wherein, yeast, milk-acid bacteria, Nitromonas, phosphorus-solubilizing bacteria, light compositing bacterium, actinomycetes, the interpolation weight ratio of growth bacterium is 1:1:1:1:1:1:1:1;
(3) sewage is removed impurity
The fecal sewage of pig farm discharge is processed to solid-liquid separating machine via ight soil and remove the impurity in sewage, obtain the sewage of removing impurity;
(4) prepare effective bacterium water quality cleansing agent
Sorrel symbiosis microorganism species is added in the sewage that step 3 removes impurity, carry out at normal temperatures oxygenation ferment 6～7 days, reaction makes its sewage be golden yellow, can obtain effective bacterium water quality cleansing agent.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480041A (en) * | 2014-12-05 | 2015-04-01 | 甘肃德福生物科技有限公司 | Microalgae liquid for purifying indoor air and application thereof |
| CN104671588A (en) * | 2015-01-06 | 2015-06-03 | 江苏理文造纸有限公司 | Method for treating pulping and papermaking wastewater |
| CN107012105A (en) * | 2016-07-11 | 2017-08-04 | 田荣国 | Composite bacteria agent, animals died of illness harmless treatment fermenting agent, composite microbe fertilizer, solid medium and fluid nutrient medium |
| CN107098482A (en) * | 2017-05-16 | 2017-08-29 | 宁波生美环保科技有限公司 | Water body cleansing agent and preparation method and application |
| CN107475143A (en) * | 2016-06-08 | 2017-12-15 | 易力源（厦门）环保科技有限公司 | Microorganism consortium for wastewater treatment |
| CN109306267A (en) * | 2017-07-26 | 2019-02-05 | 广州市紫南金母生物科技有限公司 | Application of the Jiao Su Fermentation ferment in soil sanitation |
| CN109305727A (en) * | 2017-07-26 | 2019-02-05 | 广州市紫南金母生物科技有限公司 | Application of the Jiao Su Fermentation ferment in water pollution purification |
| CN109679865A (en) * | 2018-12-25 | 2019-04-26 | 安徽佳明环保科技股份有限公司 | A kind of microorganism blender and its preparation preparation and application |
| CN111004739A (en) * | 2019-11-08 | 2020-04-14 | 深圳市星谷环保科技有限公司 | Preparation method of composite biological bacteria preparation suitable for sewage treatment |
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| CN101240239A (en) * | 2007-02-09 | 2008-08-13 | 维至康控股股份有限公司 | Composite microorganism preparation |
| CN101240238A (en) * | 2007-02-09 | 2008-08-13 | 维至康控股股份有限公司 | Method for manufacturing composition microorganism preparation |
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Patent Citations (2)
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| CN101240239A (en) * | 2007-02-09 | 2008-08-13 | 维至康控股股份有限公司 | Composite microorganism preparation |
| CN101240238A (en) * | 2007-02-09 | 2008-08-13 | 维至康控股股份有限公司 | Method for manufacturing composition microorganism preparation |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480041A (en) * | 2014-12-05 | 2015-04-01 | 甘肃德福生物科技有限公司 | Microalgae liquid for purifying indoor air and application thereof |
| CN104671588A (en) * | 2015-01-06 | 2015-06-03 | 江苏理文造纸有限公司 | Method for treating pulping and papermaking wastewater |
| CN107475143A (en) * | 2016-06-08 | 2017-12-15 | 易力源（厦门）环保科技有限公司 | Microorganism consortium for wastewater treatment |
| CN107012105A (en) * | 2016-07-11 | 2017-08-04 | 田荣国 | Composite bacteria agent, animals died of illness harmless treatment fermenting agent, composite microbe fertilizer, solid medium and fluid nutrient medium |
| CN107098482A (en) * | 2017-05-16 | 2017-08-29 | 宁波生美环保科技有限公司 | Water body cleansing agent and preparation method and application |
| CN109306267A (en) * | 2017-07-26 | 2019-02-05 | 广州市紫南金母生物科技有限公司 | Application of the Jiao Su Fermentation ferment in soil sanitation |
| CN109305727A (en) * | 2017-07-26 | 2019-02-05 | 广州市紫南金母生物科技有限公司 | Application of the Jiao Su Fermentation ferment in water pollution purification |
| CN109679865A (en) * | 2018-12-25 | 2019-04-26 | 安徽佳明环保科技股份有限公司 | A kind of microorganism blender and its preparation preparation and application |
| CN111004739A (en) * | 2019-11-08 | 2020-04-14 | 深圳市星谷环保科技有限公司 | Preparation method of composite biological bacteria preparation suitable for sewage treatment |
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Application publication date: 20140219 |