CN113354100A - Method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderately nitrifying high-concentration ammonia nitrogen wastewater - Google Patents

Method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderately nitrifying high-concentration ammonia nitrogen wastewater Download PDF

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CN113354100A
CN113354100A CN202110623891.6A CN202110623891A CN113354100A CN 113354100 A CN113354100 A CN 113354100A CN 202110623891 A CN202110623891 A CN 202110623891A CN 113354100 A CN113354100 A CN 113354100A
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霍书豪
胡新娟
王慧影
朱菲菲
钱静亚
徐玲
邹彬
王�锋
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Jiangsu University
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Abstract

The invention belongs to the field of resource engineering or biological environment engineering, and relates to a method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderately nitrifying high-concentration ammonia nitrogen wastewater; firstly, culturing filamentous microalgae species, then determining the highest ammonia nitrogen tolerance concentration of the selected filamentous microalgae, and acclimatizing and culturing the filamentous microalgae in wastewater; the wastewater after primary treatment enters a biochemical tank to be nitrified by nitrifying bacteriaModerately transforming high-concentration ammonia nitrogen to remove the toxic action and the growth inhibition action of the high-concentration ammonia nitrogen on the microalgae, and taking supernatant for culturing the filamentous microalgae after the ammonia nitrogen concentration reaches the highest tolerant concentration of the filamentous microalgae after the transformation is reduced to the highest tolerant concentration of the filamentous microalgae; CO utilization by filamentous microalgae2As a carbon source, oxygen is produced by photosynthesis; meanwhile, the biomass can be accumulated by utilizing ammonium nitrogen, nitrogen and phosphorus elements can be absorbed quickly and converted into biomass energy in a biological body, and nitrogen and phosphorus water resource recovery and carbon neutralization are realized.

Description

Method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderately nitrifying high-concentration ammonia nitrogen wastewater
Technical Field
The invention belongs to the field of resource engineering or biological environment engineering, and particularly relates to a method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling proper nitrification of high-concentration ammonia nitrogen wastewater with filamentous algae culture.
Background
Due to their unique ecological viability and higher nutrient removal capacity, microalgae are considered to be a promising method for treating sewage, such as domestic sewage, landfill leachate, agricultural wastewater (e.g., milk and meat slaughter process wastewater), and even petrochemical wastewater that is difficult to degrade. There are studies reporting that microalgae treatment systems can reduce total nitrogen and total phosphorus to 2mg/L and 0.1 mg/L. However, despite the low cost of wastewater treatment by microalgae, which does not cause secondary pollution, few microalgae-based wastewater treatment processes are currently being used in their entirety, mainly due to the harvesting of microalgae cells and the inhibition of pollutants.
Harvesting of microalgae cells and control of exogenous contaminants are major technical limitations for the scale-up of microalgae wastewater treatment. Fortunately, filamentous microalgae are easy to harvest, are not easy to be preyed by herbivores, have good pollution resistance and have obvious advantages over single-cell microalgae in biomass production harvesting and wastewater treatment. In recent years, there has been an increasing interest in the study of filamentous microalgae (e.g., Cladophora sp., Oedogonium sp., Spirogyra sp., and Tribonema sp., etc.), some of which contain high oil content and are abundant in palmitoleic acid and are considered as potential biological resources for biodiesel production.
The culture wastewater, the agricultural wastewater, the food processing wastewater and the like generally contain abundant nitrogen and phosphorus and are easily degraded by microorganisms, so that the culture wastewater is usually preferably used for culturing microalgae, but the growth of the microalgae is inhibited and even poisoned by excessively high-concentration nutrient substances, particularly ammonia nitrogen. Compared with nitrate nitrogen, ammonium nitrogen can be directly utilized by microalgae without reduction reaction. However, ammonium nitrogen is easily converted into ammonia gas, and the ammonia gas can easily penetrate cell membranes of microalgae to destroy normal cell structures, so that the low concentration of ammonia gas can inhibit the growth of the microalgae. To remove ammonia inhibition, wastewater is typically subjected to dilution treatment, but this not only consumes more water resources but also does not meet the research goal of reducing water resource consumption. In the traditional sewage treatment, ammonia nitrogen is removed mainly through physical stripping or is converted into nitrate state/nitrite nitrogen by nitrifying bacteria and then is converted into nitrogen by denitrifying bacteria to remove the nitrogen, so that energy and nitrogen element loss is caused.
Disclosure of Invention
In order to solve the problem that high-concentration ammonia nitrogen in wastewater inhibits filamentous microalgae and promote the development of microalgae in wastewater treatment and resource utilization, the invention couples a moderate nitrification technology of high-concentration ammonia nitrogen wastewater with filamentous algae culture to realize the recovery of nitrogen and phosphorus resources and carbon neutralization, and comprises the following steps:
(1) culturing filamentous algae;
preparing a BG-11 culture medium, inoculating filamentous algae into the culture medium according to aseptic operation steps after sterilization, and culturing to a logarithmic phase under the conditions of certain illumination intensity, temperature, illumination time and rotation speed for later use; in order to ensure the activity of the algae, the algae are transferred every month and cultured under the same condition;
(2) determining the highest tolerant ammonia nitrogen concentration of the selected filamentous microalgae;
collecting waste water, removing suspended matters after sedimentation, and then measuring the ammonia nitrogen and total nitrogen content in the waste water, which are respectively marked as a1mg/L and a2mg/L; ammonium chloride is used as an ammonia nitrogen source, sodium nitrate is used as a nitrate nitrogen source, and ammonia nitrogen with different gradient concentrations is preparedBG-11 medium, wherein the concentration gradient is 20-30 mg/L; after the preparation is finished, the filamentous microalgae cultured to the logarithmic phase in the step (1) are respectively inoculated into culture media with different concentrations; controlling the illumination intensity, temperature, illumination time and rotation speed, culturing for a period of time, and regularly observing and recording the color change and growth condition of the filamentous microalgae; if the concentration is marked as D under a certain concentration condition, the filamentous microalgae can keep green and die without attenuation, but in the next gradient concentration higher than the concentration D, the color of the filamentous microalgae is changed from green to light white, the growth of the filamentous microalgae is determined to stop, and the microalgae die with attenuation; in this case, the concentration D positions the highest tolerant ammonia nitrogen concentration in mg/L;
(3) domesticating filamentous microalgae;
collecting wastewater, removing suspended matters after sedimentation, taking supernatant fluid to dilute with tap water, and respectively diluting the ammonia nitrogen concentration in the target wastewater from small to large to n by taking the highest tolerant ammonia nitrogen concentration D of the filamentous microalgae determined in the step (2) as a basic value1×D，n2×D，n3×D……ni-1×D，ni8, Dmg/L, and then adjusting the pH value to 7-7.5 by using sodium hydroxide or hydrochloric acid; in the acclimatization process, firstly, filamentous microalgae is inoculated in a culture medium containing n1Culturing in ammonia nitrogen wastewater of x D mg/L until the logarithmic phase, transferring to the wastewater containing n2Culturing the XDmg/L ammonia nitrogen wastewater to logarithmic phase, gradually inoculating the XDmg/L ammonia nitrogen wastewater to high-concentration wastewater after low-concentration culture, finally transferring the XDmg/L ammonia nitrogen wastewater to the D mg/L ammonia nitrogen wastewater, and culturing filamentous microalgae to logarithmic phase, wherein the XDmg/L ammonia nitrogen wastewater is marked as domesticated filamentous microalgae; i is a positive integer greater than or equal to 2; n isiIs a positive number less than 1;
(4) ammonia nitrogen is properly converted to reduce ammonia inhibition;
collecting wastewater, adjusting the pH value to 6.5-7.5 through primary treatment, then entering a biochemical treatment system, and converting high-concentration ammonia nitrogen in the wastewater through the nitrification of sludge so as to remove the toxic action and growth inhibition action of the high-concentration ammonia nitrogen on microalgae, so that the microalgae can utilize and recover nitrogen and phosphorus in the wastewater; controlling the water temperature at 20-28 ℃, adjusting aeration quantity, controlling DO (dissolved oxygen) concentration at 1-2mg/L to provide oxygen for nitrobacteria, adjusting and maintaining the pH of wastewater at 7.0-8.0 by using sodium hydroxide in the aeration process, controlling aeration time, stopping aeration when the ammonia nitrogen concentration reaches the filamentous microalgae tolerance highest concentration D mg/L determined in the step (1) so as to avoid high energy consumption in the process of converting all ammonia nitrogen, and taking supernatant for culturing the filamentous microalgae after sludge is settled;
(5) inoculating the filamentous microalgae domesticated in the step (3) into the supernatant subjected to aeration treatment in the step (4), and setting the culture conditions to be illumination 5400Lux, the temperature to be 25-30 ℃, and the ratio of illumination to dark time to be 12: 12h, the culture container is a column reactor; CO utilization by filamentous microalgae2As a carbon source, oxygen is produced by photosynthesis; meanwhile, the biomass can be accumulated by utilizing ammonium nitrogen, nitrogen and phosphorus elements can be absorbed quickly and converted into biomass energy in a biological body, and nitrogen and phosphorus water resource recovery and carbon neutralization are realized.
Preferably, the inoculation amount of the filamentous algae in the step (1) is 0.2-0.4 g/L.
Preferably, the culture conditions in step (1) are: the illumination intensity is 5400Lux, the temperature is 25 ℃, the illumination time is 24h, and the rotating speed is 125 r/min.
Preferably, the wastewater in the step (2) is wastewater with ammonia nitrogen content higher than 200mg/L in the process of breeding, meat product or dairy product processing; the settling time is 1-2 days.
Preferably, the preparation of BG-11 medium containing ammonia nitrogen and nitrate nitrogen with different gradient concentrations in the step (2) is specifically as follows: NaNO in BG-11 Medium3The final concentration is, in order, in mg/L: (A)2-C1)×6.07，(A2-C2)×6.07，(A2-C3)×6.07……(A2-Ci+1)×6.07；
NH in BG-11 Medium4The final Cl concentration is, in order, in mg/L: c1×3.82，C2×3.82，C3×3.82……Ci+1×3.82；
Wherein C is1The initial concentration of ammonia nitrogen is 20-30 mg/L; ci+1＝A1Namely the ammonia nitrogen concentration in the target sewage; ci+1-Ci＝20～50mg/L；A2Is the total nitrogen concentration in the target sewage; 6.07 is nitrogen and NaNO33.82 is the conversion coefficient of nitrogen element and ammonium chloride; i is a positive integer of 2 or more.
Preferably, the culture conditions in step (2) are: the illumination intensity is 5400Lux, the temperature is 25-30 ℃, the illumination time is 12-24 h, and the rotating speed is 125 revolutions per minute.
Preferably, the period of culturing in step (2) is 9 days, and the regular observation is performed every 12 hours.
Preferably, the wastewater in the step (3) is wastewater with ammonia nitrogen content higher than 200mg/L in the process of breeding, meat product or dairy product processing; the settling time is 1-2 days; the inoculation amount of the filamentous algae is 0.2-0.4 g/L.
Preferably, the inoculation amount in the step (5) is 0.2-0.4 g/L.
A large amount of wastewater containing high-concentration ammonia nitrogen is generated in the industries of cultivation, printing and dyeing and the like, and although the traditional treatment means can effectively remove nutrient substances such as nitrogen and phosphorus, the defects of high aeration energy consumption, large occupied area, need of an additional carbon source as an electron donor and the like exist.
Compared with the prior art, the invention has the following beneficial effects:
the invention carries out moderate aeration control on the high ammonia nitrogen concentration wastewater, realizes that ammonia nitrogen does not completely meet the condition of converting into nitrite state and nitrate nitrogen, and greatly reduces aeration energy consumption; the method is easy to collect filamentous microalgae and preferentially uses ammonium nitrogen, and compared with the method for using nitrate nitrogen, the method has the advantages of shorter metabolic flow and less required energy; without additional carbon source, CO is utilized2As a carbon source, filamentous algae generate oxygen through photosynthesis, and partially replace aeration equipment, so that the dissolved oxygen content in sewage is increased, and the anaerobic denitrification process is inhibited; filamentous algae converts N, P and other elements into biomass energy in a living body, can be used as a raw material of products with economic value, such as compost and feed, and has high recovery value; at the same time, photosynthesis of microalgae absorbs CO2And the pH value in the sewage is increased, and a certain disinfection and bacteriostasis effect can be achieved.
Drawings
FIG. 1 shows the growth rate and pollutant removal rate of Bothrix fulvus when the ammonia nitrogen concentration is 120 mg/L.
FIG. 2 is a histogram of the components of Bothrix fulvescens at an ammonia nitrogen concentration of 120 mg/L.
FIG. 3 shows the growth rate and pollutant removal rate of Bothrix fulvus when the ammonia nitrogen concentration is 90 mg/L.
FIG. 4 is a histogram of the components of Bothrix fulvescens at 90mg/L ammonia nitrogen concentration.
Detailed Description
The invention is further described with reference to the drawings and the detailed description. The high ammonia nitrogen concentration wastewater refers to that the ammonia nitrogen concentration is not less than 100 mg/L.
Wherein, the formula of the BG-11 culture medium is shown in table 1 and table 2;
TABLE 1 preparation of BG-11
Figure BDA0003100293580000041
TABLE 2 solution of trace elements
Figure BDA0003100293580000042
Example 1:
(1) cultivation of filamentous algae
Inoculating filamentous algae to sterilized BG-11 culture medium (formula shown in Table 1 and Table 2) according to aseptic operation, controlling illumination intensity at 5400Lux, temperature at 25 deg.C, illumination time 24h, and rotation speed at 125 rpm, and culturing to logarithmic phase for use; (to ensure the activity of the algae, the algae are transferred every month, namely, the algae are transferred into a fresh BG-11 culture medium and cultured under the same condition);
(2) collecting the aquaculture wastewater, removing suspended matters after settling for 2 days, and determining the content of ammonia nitrogen and the total nitrogen to be 210mg/L and 240mg/L respectively. Preparing BG-11 culture medium containing different ammonia nitrogen concentrations by using ammonium chloride as ammonia nitrogen source and sodium nitrate as nitrate nitrogen source, and setting ammonia nitrogen initial concentration C130mg/L, concentration gradient Ci+1-Ci＝30mg/L, gradually increasing the ammonia nitrogen concentration to A1210mg/L, total nitrogen concentration A2Respectively inoculating 240mg/L filamentous microalgae cultured to the logarithmic phase in the step (1), wherein the inoculation amount is 0.2 g/L; culturing under 5400Lux illumination intensity, temperature of 25 deg.C, illumination time of 24h, 125 rpm for 9d, observing color change and growth of filamentous microalgae, and finding yellow filamentous microalgae with ammonia nitrogen concentration higher than 120mg/L to turn white and die on day 3. Therefore, the highest ammonia nitrogen concentration D which the yellow silk algae can tolerate is 120 mg/L;
(3) domesticating filamentous microalgae;
collecting aquaculture wastewater, removing suspended matters after settling for 2 days, taking supernatant, diluting with tap water, diluting the ammonia nitrogen concentration in the target wastewater to 6mg/L, inoculating 0.2g/L of Aphanizomenon flavipes, culturing for 3 days to reach logarithmic phase, transferring the Aphanizomenon flavipes to wastewater with the ammonia nitrogen concentration of 12mg/L, sequentially transferring the Aphanizomenon flavipes to wastewater with the ammonia nitrogen concentrations of 24, 48 and 96mg/L after culturing for 3 days to logarithmic phase, transferring the Aphanizomenon flavipes to wastewater with the highest ammonia nitrogen concentration (120mg/L) tolerant after culturing for 96mg/L wastewater to logarithmic phase, and culturing filamentous microalgae to the logarithmic phase under the ammonia nitrogen with the ammonia nitrogen concentration;
(4) setting technical parameters of wastewater aeration oxidation;
controlling the water temperature at 25 ℃, mixing the culture wastewater with activated sludge, then carrying out aeration treatment by using an air compression pump, adjusting the aeration amount to 72L/h, controlling the DO (dissolved oxygen) concentration to 2mg/L to provide sufficient oxygen for nitrobacteria, adjusting and maintaining the pH of the wastewater to 7.5 +/-0.5 by using sodium hydroxide in the aeration process, stopping aeration when the ammonia nitrogen concentration reaches the maximum concentration of 120mg/L tolerated by the filamentous microalgae determined in the step (1) after 5 hours of aeration, standing and precipitating to obtain a supernatant;
(5) inoculating the yellow silk algae domesticated in the step (3) into the supernatant subjected to aeration treatment in the step (4), wherein the inoculation amount is 0.2g/L, and the culture conditions are that the illumination is 5400Lux, the temperature is 25 ℃, and the illumination time is 12: 12h, the culture container is a column reactor; CO utilization by filamentous microalgae2As a carbon source, oxygen is produced by photosynthesis; simultaneously, the biomass accumulation can be carried out by utilizing ammonium nitrogen, and the biomass can be absorbed quicklyNitrogen and phosphorus elements are converted into biomass energy in the organism, and nitrogen and phosphorus water resource recovery and carbon neutralization are realized.
As shown in figure 1, under the condition of moderate aeration, the chrysophyceae can accumulate biomass by using a proper amount of ammonium nitrogen, can tolerate 120mg/L of ammonia nitrogen, and can rapidly absorb elements such as nitrogen and phosphorus, so that, the analysis of total nitrogen, ammonia nitrogen and total phosphorus shows that under the condition of moderate aeration, the xanthomonas can quickly absorb nitrogen elements for growth, as shown in figure 1, the total nitrogen removal rate can reach 61.97%, the degradation of ammonia nitrogen by the Aphanizomenon flavipes is also analyzed, the initial ammonia nitrogen concentration is 120mg/L, the ammonia nitrogen is reduced to 28.47mg/L and 76.47% is reduced after 9d culture (figure 1), the microalgae can absorb phosphorus, synthesize protein and other substances, therefore, the phosphorus element in the culture system is analyzed and determined (figure 1), and the removal rate of the phosphorus can reach more than 85 percent; the xanthomonas can absorb the biomass energy in the organism synthesized by elements such as nitrogen and phosphorus in the wastewater, and as shown in fig. 2, the research finds that when the ammonia nitrogen value is 120mg/L, the content of the xanthomonas polysaccharide is 30.84%, and the content of the grease is 50.8%.
Example 2:
(1) culturing filamentous algae;
inoculating the Aphanizomenon chrysosporium into a BG-11 culture medium (formula shown in table 1 and table 2) according to aseptic operation steps, controlling illumination intensity to be 5400Lux, temperature to be 25 ℃, illumination time to be 24h, and rotating speed to be 125 r/min; (to ensure the activity of the algal cells, the algal cells were transferred every month, i.e., transferred to fresh BG-11 medium and cultured under the same conditions).
(2) Collecting the culture wastewater, removing suspended matters after settling for 1 day, and determining the ammonia nitrogen content and the total nitrogen content to be 187mg/L and 221mg/L respectively. Preparing BG-11 culture medium containing different ammonia nitrogen concentrations by using ammonium chloride as ammonia nitrogen source and sodium nitrate as nitrate nitrogen source, and setting ammonia nitrogen initial concentration C130mg/L, concentration gradient Ci+1-CiIncreasing the ammonia nitrogen concentration gradient to A at 30mg/L1180mg/L, total nitrogen concentration A2220mg/L, inoculating 0.2g/L of filamentous microalgae in log phase. Lux at 5400Culturing for 9 days under the conditions of illumination intensity, temperature of 25 ℃, illumination time of 24h and 125 r/min, observing color change and growth condition of the filamentous microalgae, finding that the yellow filamentous microalgae is yellow when the ammonia nitrogen concentration is higher than 90mg/L, and the microalgae is white and dies on day 3. Therefore, the highest ammonia nitrogen concentration D which the yellow silk algae can tolerate is 90 mg/L.
(3) Domesticating filamentous microalgae;
collecting aquaculture wastewater, removing suspended matters after settling for 1-2 days, taking supernatant, diluting with tap water, diluting the ammonia nitrogen concentration in the target wastewater to 6mg/L, inoculating 0.2g/L of Aphanizomenon flavipes, culturing for 3 days to reach logarithmic phase, transferring to wastewater with ammonia nitrogen concentration of 12mg/L, culturing for 3 days to logarithmic phase, sequentially transferring to wastewater with ammonia nitrogen concentration of 24 and 48mg/L, transferring to wastewater with highest ammonia nitrogen concentration (96mg/L) tolerated by Aphanizomenon flavipes, and culturing filamentous microalgae to logarithmic phase under the ammonia nitrogen with the ammonia nitrogen concentration.
(4) Setting technical parameters of wastewater aeration oxidation;
controlling the water temperature at 25 ℃, mixing the culture wastewater with activated sludge, then carrying out aeration treatment by using an air compression pump, adjusting the aeration amount to 72L/h, controlling the DO (dissolved oxygen) concentration to 2mg/L to provide sufficient oxygen for nitrobacteria, adjusting and maintaining the pH of the wastewater to 7.5 +/-0.5 by using sodium hydroxide in the aeration process, stopping aeration when the ammonia nitrogen concentration reaches the maximum concentration of 120mg/L tolerated by the filamentous microalgae determined in the step (1) after 5 hours of aeration, standing and precipitating to obtain a supernatant;
(5) inoculating the yellow silk algae domesticated in the step (3) into the supernatant subjected to aeration treatment in the step (4), wherein the inoculation amount is 0.2g/L, and the culture conditions are that the illumination is 5400Lux, the temperature is 25 ℃, and the illumination time is 12: 12h, the culture container is a column reactor. CO utilization by filamentous microalgae2As a carbon source, oxygen is produced by photosynthesis; meanwhile, the biomass can be accumulated by utilizing ammonium nitrogen, nitrogen and phosphorus elements can be absorbed quickly and converted into biomass energy in a biological body, and nitrogen and phosphorus water resource recovery and carbon neutralization are realized.
As shown in figure 1, under the condition of moderate aeration, the Aphanizomenon flavipes can accumulate biomass by using a proper amount of ammonium nitrogen (as shown in figure 3), when the ammonia nitrogen concentration is 90mg/L, the biomass is 4.68g/L, the Aphanizomenon flavipes can absorb elements such as nitrogen, phosphorus and the like for accumulation of the biomass, as shown in the result of figure 3, the initial nitrogen concentration is 240mg/L, and the total nitrogen removal rate reaches 67.24% after 9d culture; the ammonia nitrogen removal rate can reach 85.46%; the removal rate of total phosphorus can reach 88.03%; the content of total sugar in the xanthoceras fulva was 34.42%, and the content of oil and fat was 48.2% (fig. 4).
Description of the drawings: the above embodiments are only used to illustrate the present invention and do not limit the technical solutions described in the present invention; thus, while the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted; all such modifications and variations are intended to be included herein within the scope of this disclosure and the present invention and protected by the following claims.

Claims (8)

1. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderately nitrifying high-concentration ammonia nitrogen wastewater is characterized by comprising the following preparation steps of:
(1) culturing filamentous algae;
firstly, preparing a BG-11 culture medium containing sodium nitrate, inoculating filamentous algae in the culture medium according to an aseptic operation step after the culture medium is sterilized, and culturing the filamentous algae to a logarithmic phase under the conditions of certain illumination intensity, temperature, illumination time and rotation speed for later use;
(2) determining the highest tolerant ammonia nitrogen concentration of the selected filamentous microalgae;
collecting waste water, removing suspended matters after sedimentation, and then measuring the ammonia nitrogen and total nitrogen content in the waste water, which are respectively marked as a1mg/L and a2mg/L; preparing BG-11 culture media containing different gradient ammonia nitrogen concentrations by taking ammonium chloride as an ammonia nitrogen source and sodium nitrate as a nitrate nitrogen source, wherein the concentration gradient is 20-30 mg/L; after the preparation is finished, the filamentous microalgae cultured to the logarithmic phase in the step (1) are respectively inoculated into culture media with different concentrations; controlling illumination intensity, temperature, illumination time and rotation speed, culturing for a period of time, and periodically observing and recording color change of filamentous microalgaeChemolysis and growth conditions; if the concentration is marked as D under a certain concentration condition, the filamentous microalgae can keep green and die without attenuation, but in the next gradient concentration higher than the concentration D, the color of the filamentous microalgae is changed from green to light white, the growth of the filamentous microalgae is determined to stop, and the microalgae die with attenuation; in this case, the concentration D positions the highest tolerant ammonia nitrogen concentration in mg/L;
(3) domesticating filamentous microalgae;
collecting wastewater, removing suspended matters after sedimentation, taking supernatant fluid to dilute with tap water, and respectively diluting the ammonia nitrogen concentration in the target wastewater from small to large to n by taking the highest tolerant ammonia nitrogen concentration D of the filamentous microalgae determined in the step (2) as a basic value1×D，n2×D，n3×D……ni-1×D，ni8, Dmg/L, and then adjusting the pH value to 7-7.5 by using sodium hydroxide or hydrochloric acid; in the acclimatization process, firstly, filamentous microalgae is inoculated in a culture medium containing n1Culturing in ammonia nitrogen wastewater of x D mg/L until the logarithmic phase, transferring to the wastewater containing n2Culturing the XDmg/L ammonia nitrogen wastewater to logarithmic phase, gradually inoculating the XDmg/L ammonia nitrogen wastewater to high-concentration wastewater after low-concentration culture, finally transferring the XDmg/L ammonia nitrogen wastewater to the D mg/L ammonia nitrogen wastewater, and culturing filamentous microalgae to logarithmic phase, wherein the XDmg/L ammonia nitrogen wastewater is marked as domesticated filamentous microalgae; i is a positive integer greater than or equal to 2; n isiIs a positive number less than 1;
(4) ammonia nitrogen is properly converted to reduce ammonia inhibition;
collecting wastewater, adjusting the pH value to 6.5-7.5 through primary treatment, then entering a biochemical treatment system, and converting high-concentration ammonia nitrogen in the wastewater through the nitrification of sludge so as to remove the toxic action and growth inhibition action of the high-concentration ammonia nitrogen on microalgae, so that the microalgae can utilize and recover nitrogen and phosphorus in the wastewater; controlling the water temperature at 20-28 ℃, adjusting aeration amount, controlling the dissolved oxygen concentration at 1-2mg/L to provide oxygen for nitrifying bacteria, adjusting and maintaining the pH of the wastewater at 7.0-8.0 by using sodium hydroxide in the aeration process, controlling aeration time, stopping aeration when the ammonia nitrogen concentration reaches the highest tolerant concentration D mg/L of the filamentous microalgae determined in the step (1), and taking supernatant for culturing the filamentous microalgae after sludge is settled;
(5) taking stepsInoculating the filamentous microalgae domesticated in the step (3) into the supernatant subjected to aeration treatment in the step (4), and setting culture conditions to be light illumination 5400Lux, temperature of 25-30 ℃, and ratio of light illumination to dark time to be 12: 12h, the culture container is a column reactor; CO utilization by filamentous microalgae2As a carbon source, oxygen is produced by photosynthesis; meanwhile, the biomass can be accumulated by utilizing ammonium nitrogen, nitrogen and phosphorus elements can be absorbed quickly and converted into biomass energy in a biological body, and nitrogen and phosphorus water resource recovery and carbon neutralization are realized.
2. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of high-concentration ammonia nitrogen wastewater according to claim 1, wherein the inoculation amount of the filamentous algae in the step (1) is 0.2-0.4 g/L.
3. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of the high-concentration ammonia-nitrogen wastewater according to claim 1, wherein the culture conditions in the step (1) are as follows: the illumination intensity is 5400Lux, the temperature is 25 ℃, the illumination time is 24h, and the rotating speed is 125 r/min.
4. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of the high-concentration ammonia nitrogen wastewater according to claim 1, wherein the wastewater in the step (2) is the wastewater with the ammonia nitrogen content higher than 200mg/L in the process of cultivation, meat product or dairy product processing; the settling time is 1-2 days.
5. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of high-concentration ammonia nitrogen wastewater according to claim 1, wherein the preparation of BG-11 culture media with different gradient ammonia nitrogen concentrations in the step (2) specifically comprises the following steps: NaNO in BG-11 Medium3The final concentration is, in order, in mg/L: (A)2-C1)×6.07，(A2-C2)×6.07，(A2-C3)×6.07……(A2-Ci+1)×6.07；
NH in BG-11 Medium4The final Cl concentration is, in order, in mg/L: c1×3.82，C2×3.82，C3×3.82……Ci+1×3.82；
Wherein C is1The initial concentration of ammonia nitrogen is 20-30 mg/L; ci+1＝A1Namely the ammonia nitrogen concentration in the target sewage; ci+1-Ci＝20～50mg/L；A2Is the total nitrogen concentration in the target sewage; 6.07 is nitrogen and NaNO33.82 is the conversion coefficient of nitrogen element and ammonium chloride; i is a positive integer of 2 or more.
6. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of the high-concentration ammonia-nitrogen wastewater according to claim 1, wherein the culture conditions in the step (2) are as follows: the illumination intensity is 5400Lux, the temperature is 25-30 ℃, the illumination time is 12-24 h, and the rotating speed is 125 revolutions per minute; the culture period is 9 days, and the regular observation is specifically carried out every 12 hours.
7. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of the high-concentration ammonia nitrogen wastewater according to claim 1, wherein the wastewater in the step (3) is the wastewater with the ammonia nitrogen content higher than 200mg/L in the process of cultivation, meat product or dairy product processing; the settling time is 1-2 days; the inoculation amount of the filamentous algae is 0.2-0.4 g/L.
8. The method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderate nitrification of the high-concentration ammonia-nitrogen wastewater according to claim 1, wherein the inoculation amount in the step (5) is 0.2-0.4 g/L.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114804328A (en) * 2022-05-24 2022-07-29 中国科学院广州能源研究所 Method for assimilating and recycling high ammonia nitrogen wastewater by partially and completely nitrifying microalgae
CN115232751A (en) * 2022-07-25 2022-10-25 武汉轻工大学 Method for enhancing nitrogen removal capability of Bothrix fulvescens by utilizing low-nitrogen pretreatment, nitrogen treatment method and Bothrix fulvescens obtained

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114804328A (en) * 2022-05-24 2022-07-29 中国科学院广州能源研究所 Method for assimilating and recycling high ammonia nitrogen wastewater by partially and completely nitrifying microalgae
CN115232751A (en) * 2022-07-25 2022-10-25 武汉轻工大学 Method for enhancing nitrogen removal capability of Bothrix fulvescens by utilizing low-nitrogen pretreatment, nitrogen treatment method and Bothrix fulvescens obtained
CN115232751B (en) * 2022-07-25 2023-12-22 武汉轻工大学 Method for enhancing denitrification capacity of Phlebophyta by low-nitrogen pretreatment, nitrogen treatment method and obtained Phlebophyta

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