CN102070283B - Technique for efficiently treating livestock/poultry culture wastewater - Google Patents

Technique for efficiently treating livestock/poultry culture wastewater Download PDF

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CN102070283B
CN102070283B CN2010105942519A CN201010594251A CN102070283B CN 102070283 B CN102070283 B CN 102070283B CN 2010105942519 A CN2010105942519 A CN 2010105942519A CN 201010594251 A CN201010594251 A CN 201010594251A CN 102070283 B CN102070283 B CN 102070283B
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livestock
trichoderma
sewage
poultry
bead
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CN102070283A (en
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范子山
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范子山
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Abstract

The invention discloses a technique for efficiently treating livestock/poultry culture wastewater. The technique comprises the following steps: carrying out anaerobe-aerobe treatment to firstly remove large suspended substances in sewage and then remove fine suspended substances and sludge; and after finishing physical removal, carrying out microbial treatment to remove colibacillus, roundworm eggs and other pathogenic organisms, and heavy metals and other pollutants in the sewage, thus about 90% of harmful substances in the raw sewage can be removed. Anaerobes and Trichoderma can be used to catalyze and ferment ammonia, alcohol sulfide, hydrogen sulfide and the like, and convert and decompose ammonia, alcohol sulfide, hydrogen sulfide and the like into nontoxic and harmless compounds, thereby achieving the goals of treating pollution, removing odor, reducing ammonia content and purifying the environment.

Description

A kind of technology of efficient processing livestock breeding wastewater
Technical field
The present invention relates to a kind of wastewater processing technology, definite a kind of treatment process that is used for livestock breeding wastewater of saying so.
Background technology
Be accompanied by livestock and poultry breeding industry to mass-producing, intensivization development, the animal waste total amount is growing trend year by year, and livestock and poultry breeding industry pollutes has become one of principal element of rural area pollution of area source.State Environmental Protection Administration finds that to the investigation of 23 provinces and cities in the whole nation 90% livestock and poultry farms in scale does not pass through environmental impact assessment, and 60% plant lacks necessary pollution prevention measure.According to measuring and calculating, adult chicken 0.11kg that approximately voided excreta in a day, 100,000 chickens were discharged the about 4000t of fecaluria in 1 year.Plant's (by delivering calculating in 6 months for sale) blowdown flow rate of every day of producing 10000 pigs per year is equivalent to BOD (biochemical oxygen demand the is claimed biochemical oxygen demand again) value of 50,000 human excrement.Some provinces and cities encourage scale animal and poultry cultivation as industry restructuring, the important channel of increasing farmers' income, but ignored prevention and cure of pollution work.Annual waste water by the livestock and poultry farm discharging, near the COD total emission volumn of trade effluent, the solids of discharging is 4 times of industrial solid castoff to its COD (chemical oxygen demand) total amount, reaches 1,900,000,000 t.Wherein nitrogen contracts amount is about 1,597 ten thousand t, and phosphorus contracts amount is about 3,630,000 t, and nitrogen, phosphorus loss amount are greater than the loss of chemical fertilizer amount.The pollution that livestock and poultry cultivation produces has become the geographic primary pollution source of Chinese countryside, deals with improperly ecotope is produced grave danger, has a strong impact on the Sustainable development of ecotope and society.
Utilizing the mikrobe of biological safety to handle livestock and poultry cultivation sewage and have advantages such as nontoxic, noresidue, non-secondary pollution, eliminate beyond doubt and a kind of safe, effective, the inexpensive method of detoxifcation pesticide residue, also is a main striving direction in recent years.Trichoderma ( Trichoderma) be to use biological control agents the most widely in the world, the application of in the control of various crop disease, having succeeded.The mould prussiate of can degrading of wood (Ezzi and Lynch, 2005; Zhou et al., 2007), (Sanna et al., 1997 such as agricultural chemicals (Fu Wenxiang, 2005), formaldehyde (Sawada et al., 2006), diesel oil (Nwaogu et al., 2008), phenols (Karetnikova and Zhirkova, 2005) and absorption heavy metal; Yazdania et al., 2009).We find that also Trichoderma can utilize multiple organism as carbon source, nitrogenous source or phosphorus source at research; Yeast FJ1 contains gene of flocculating, and the muddy thing rapid precipitation of sewage is got off.
Adopt microbial immobilized technology in bio-reactor is used, to have the following advantages: more or less freely realization solid-liquid separation, to increase the resistance of environment to external world and usability repeatedly.In addition; Thereby immobilization technology can keep higher microbial bacteria bulk concentration to guarantee in entire reaction course to obtain higher efficient, makes in the entering microorganism cells reactive system that external substance is more prone to thereby immobilization simultaneously can change the permeability of microorganism cells film.The crosslinked fixation method of alginate calcium becomes the technology of the most frequently used a kind of fixation of microbe active somatic cell owing to simple, the nontoxic and relative mildness of its making processes.But this important techniques is not applied to utilize the biological fixnig bacterial strain to handle livestock and poultry cultivation sewage aspect so far as yet.
Summary of the invention
The present invention is intended to set up an immobilization system that is fit to handle livestock and poultry cultivation sewage, realizes quicker, effective, safe processing livestock and poultry cultivation sewage.
Also do not have at present to find about relevant report of the present invention, the research of inventor's process TE, the successful finally sewage disposal technology of exploring a kind of efficient processing livestock and poultry cultivation, thus accomplished the present invention.
The method that the purpose of this invention is to provide a kind of efficient processing livestock and poultry cultivation sewage realizes qualified discharge, with improvement environment and realization Sustainable development.
The present invention with the biological safety anerobes become reconciled bacteria strain wood mould with yeast be main bacterial strain, through immobilization embedded technology, realize the efficient sewage of repairing livestock and poultry cultivation.
Described anerobes (anaerobicbacteria) be one type under oxygen free condition than the good bacterium of growth in aerobic environment, and can not air (18% oxygen) with (or) 10% gas concentration lwevel solid medium down shows the bacterium that grows.Trichoderma is in Deuteromycotina, Moniliales, and Trichoderma, common wooden mouldly have viride, healthy and free from worry wood mould etc.Wood is mould to have than strong decomposition of cellulose ability, and viride can produce the cellulase of high activity usually, and is very strong to cellulosic resolving power.Yeast is some unicellular fungies, is not the unit of phylogeny classification.Yeast is the mikrobe that is employed the earliest in the human civilization history, can in anaerobic environment, survive.
The present invention dispels suspended substance big in the sewage earlier through the anaerobic-aerobic microbiological treatment, dispels thin suspended substance and mud again.After accomplishing the dispeling of physical property, utilize microbiological treatment again, dispel pollutents such as causal organism such as intestinal bacteria in the sewage, roundworm egg and heavy metal.Through after such processing, about 90% objectionable impurities can both be removed in the raw waste water.Anerobes and Trichoderma can carry out catalysis and fermentation to ammonia, sulfurized alcohol, hydrogen sulfide etc.; Transform and be decomposed into nontoxic, harmless compound; Reach the pollution treatment deodorizing and fall the purpose of ammonia environment purification, have high efficiency, specificity, reaction conditions gentleness and variety characteristics.
Treatment process of the present invention specifically may further comprise the steps
1, Trichoderma and saccharomycetic large scale culturing
Activation inoculation on the slant medium that Trichoderma FM1 and yeast FJ1 preserve from refrigerator, with 25-30 ℃ of nutritious potato glucose liquid nutrient medium, rotating speed is that the shaking table concussion cultivation of 200 rpm makes its expansion numerous.After cultivating 3d, use rotating speed to be used for cell fixation as 8000rpm whizzer collection Trichoderma silk and saccharomycetic thalline.
2, Trichoderma silk and saccharomycetic embedding
Fresh Trichoderma silk and yeast join in 3% sodium-alginate and 1% zeyssatite in the ratio of 1:20, and 200rpm concussion 4h makes mycelia and sodium-alginate suspension thorough mixing.Draw suspension then, at the uniform velocity splash into 3%CaCl 2In the aqueous solution, at drop during near the water surface, sodium-alginate rapidly and Ca 2+Reaction forms the bead of alginate calcium parcel.Behind 4 ℃ of sclerosis of controlled temperature 4h, take out bead, subsequent use.
3, the quick solid-liquid separation of waste water of livestock poultry
After the waste water of livestock and poultry cultivation discharging reclaims through special-purpose collecting tank; The yeast bead that adds the alginate calcium parcel that fixes; The clarification that can in 5-10min, become fast of muddy sewage is collected solid and is thoroughly killed harmful microorganisms such as roundworm egg and intestinal bacteria to 80 ℃ of pyroprocessing of High Temperature Sterilization machine warp.
4, anaerobically fermenting
Change the filtrating after the solid-liquid separation over to the anaerobically fermenting pond, anerobes can be decomposed into dissolved organic matter or inorganics to insoluble organism, realizes innoxious production, thereby reaches the purpose of environment purification.Feces of livestock and poultry is through the sealing anaerobically fermenting of long period, also directly kill pathogens and parasite, minimizing biological pollution.
5, wooden mould processing
In the waste water that anaerobically fermenting is handled, add immobilized Trichoderma silk bead, behind the 24h, reclaim the mycelia bead.Sewage to after handling carries out COD, BOD, NH 3Indexs of correlation such as-N, total P, coliform group count, roundworm egg number are measured, and meet " livestock and poultry breeding industry pollutant emission standard " and directly discharge after (GB18596).
Manufacture craft of the present invention is simple, and is easy to operate, utilizes biotechnology to handle livestock and poultry cultivation sewage, and efficient is high, and speed is fast.
Description of drawings:
Fig. 1 is a process flow sheet of the present invention.
Embodiment
Embodiment 1:
1, Trichoderma and saccharomycetic large scale culturing
Get activatory fresh Trichoderma FM1 spore and yeast FJ1 respectively to nutritious potato glucose liquid nutrient medium, the initial inoculation amount is by 10 7Individual spore (bacterium)/milliliter, temperature is 28 ℃, rotating speed is that the shaking table concussion cultivation of 200rpm makes its expansion numerous.After cultivating 3d, use rotating speed to be used for cell fixation as 8000rpm whizzer collection Trichoderma silk and saccharomycetic thalline.
2, Trichoderma silk and saccharomycetic embedding
Fresh Trichoderma silk and yeast join in 3% sodium-alginate and 1% zeyssatite in the ratio of 1:20, and 200rpm shakes 4h, make mycelia and sodium-alginate suspension thorough mixing.Draw suspension then, at the uniform velocity splash into 3%CaCl 2In the aqueous solution, at drop during near the water surface, sodium-alginate rapidly and Ca 2+Reaction forms the bead of alginate calcium parcel.Behind 4 ℃ of sclerosis of controlled temperature 4h, take out bead, subsequent use.
3, the quick solid-liquid separation of waste water of livestock poultry
After the waste water of livestock and poultry cultivation discharging reclaims through special-purpose collecting tank; The yeast bead that adds the Protanal TXF 200 parcel that fixes; The clarification that in 10min, becomes fast of muddy sewage is collected solid and is thoroughly killed harmful microorganisms such as roundworm egg and intestinal bacteria to 80 ℃ of pyroprocessing of High Temperature Sterilization machine warp.
4, anaerobically fermenting
Change the filtrating after the solid-liquid separation over to the anaerobically fermenting pond, anaerobically fermenting 15d, anerobes can be decomposed into dissolved organic matter or inorganics to insoluble organism, realizes innoxious production, thereby reaches the purpose of environment purification.Feces of livestock and poultry is through the sealing anaerobically fermenting of long period, also directly kill pathogens and parasite, minimizing biological pollution.
5, wooden mould processing
In the waste water that anaerobically fermenting is handled, add immobilized Trichoderma silk bead, behind the 24h, reclaim the mycelia bead.Sewage after handling is carried out index of correlation measure the SS of externally discharged waste water, COD, BOD, NH 3-N, total P, coliform group count, roundworm egg number are respectively 120mg/L, 150mg/L, 110mg/L, 60mg/L, 5.1mg/L, 500/100mL, 0.5/L, meet " livestock and poultry breeding industry pollutant emission standard " (GB18596).
Attach: State Standard of the People's Republic of China GB18596-2001 livestock and poultry breeding industry pollutant emission standard

Claims (3)

1. efficient method of handling livestock breeding wastewater, it is characterized in that: it comprises the following steps:
(1) Trichoderma and saccharomycetic large scale culturing
The slant medium activation inoculation respectively that Trichoderma FM1 and yeast FJ1 preserve from refrigerator; With 25-30 ℃ of nutritious potato glucose liquid nutrient medium; Rotating speed is that the shaking table concussion cultivation of 200 rpm makes its expansion numerous; After cultivating 3d, use rotating speed to be used for cell fixation as 8000rpm whizzer collection Trichoderma silk and saccharomycetic thalline;
(2) Trichoderma silk and saccharomycetic embedding
Fresh Trichoderma silk and yeast join in 3% sodium-alginate and 1% zeyssatite in the ratio of 1:20, and 200rpm concussion 4h makes mycelia and sodium-alginate suspension thorough mixing, draws suspension then, at the uniform velocity splashes into 3%CaCl 2In the aqueous solution, at drop during near the water surface, sodium-alginate rapidly and Ca 2+Reaction forms the bead of alginate calcium parcel, behind 4 ℃ of sclerosis 4h, takes out bead, and is subsequent use;
(3) the quick solid-liquid separation of waste water of livestock poultry
After the waste water of livestock and poultry cultivation discharging reclaims through special-purpose collecting tank; The yeast bead that adds the alginate calcium parcel; The clarification that can in 5-10min, become fast of muddy sewage is collected solid and is thoroughly killed roundworm egg and intestinal bacteria harmful microorganism to 80 ℃ of pyroprocessing of High Temperature Sterilization machine warp;
(4) anaerobically fermenting
Change the filtrating after the solid-liquid separation over to the anaerobically fermenting pond; Anerobes can be decomposed into dissolved organic matter or inorganics to insoluble organism; Realize innoxious production, thereby reach the purpose of environment purification that feces of livestock and poultry is through the sealing anaerobically fermenting of long period; Also directly kill pathogens and parasite reduce biological pollution;
(5) wooden mould processing
In the waste water that anaerobically fermenting is handled, add immobilized Trichoderma silk bead, behind the 24h, reclaim the mycelia bead, the sewage after handling is carried out COD, BOD, NH 3-N, total P, coliform group count, roundworm egg number index of correlation are measured, and directly discharge after meeting livestock and poultry breeding industry pollutant emission standard GB18596.
2. the method for efficient processing livestock breeding wastewater according to claim 1 is characterized in that: get activatory fresh Trichoderma FM1 spore and yeast FJ1 to nutritious potato glucose liquid nutrient medium, the initial inoculation amount is by 10 7Individual spore/milliliter, temperature are 28 ℃, and rotating speed is that the shaking table concussion cultivation of 200rpm is expanded numerous.
3. the method for efficient processing livestock breeding wastewater according to claim 1 is characterized in that: the sewage after handling is carried out index of correlation measure the SS of externally discharged waste water, COD, BOD, NH 3-N, total P, coliform group count, roundworm egg number are respectively 120mg/L, 150mg/L, 110mg/L, 60mg/L, 5.1mg/L, 500/100mL, 0.5/L.
CN2010105942519A 2010-12-19 2010-12-19 Technique for efficiently treating livestock/poultry culture wastewater Expired - Fee Related CN102070283B (en)

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CN103357653A (en) * 2013-07-23 2013-10-23 邓锦新 Household refuse and domestic sewage synchronous processing method
CN104326561B (en) * 2014-11-18 2016-04-20 华中农业大学 A kind of multistage microbial augmentation improves the method for livestock breeding wastewater processing efficiency
CN110153177B (en) * 2019-03-08 2020-07-31 中国科学院广州地球化学研究所 Method for repairing polycyclic aromatic hydrocarbon polluted soil by using fungi
CN109896639A (en) * 2019-04-03 2019-06-18 深圳市清研环境科技有限公司 Microbial inoculum, preparation method and its application in sewage treatment of resistance to heavy metal

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Application publication date: 20110525

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