CN111004739A - Preparation method of composite biological bacteria preparation suitable for sewage treatment - Google Patents
Preparation method of composite biological bacteria preparation suitable for sewage treatment Download PDFInfo
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- CN111004739A CN111004739A CN201911086377.2A CN201911086377A CN111004739A CN 111004739 A CN111004739 A CN 111004739A CN 201911086377 A CN201911086377 A CN 201911086377A CN 111004739 A CN111004739 A CN 111004739A
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- bacteria
- composite biological
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- sewage treatment
- flora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention discloses a preparation method of a composite biological bacteria preparation suitable for sewage treatment, which comprises the following steps: (1) taking filtered purified water and adding micro aeration for carrying out aerobic treatment for the first time to obtain oxygen-enriched filtered purified water; adding a carbon source into the oxygen-enriched filtered purified water, and uniformly mixing to obtain a culture solution for later use; (2) adding a compound biological strain into the culture solution in the step (1), wherein the compound biological strain comprises a mixture of photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes, bacillus, nitrobacteria, phosphorus dissolving bacteria and nitrogen fixing bacteria; (3) and adding micro-aeration into the culture solution added with the compound biological strain to perform secondary aerobic treatment, and culturing the compound biological strain to obtain the required compound biological bacteria preparation. The preparation method of the composite biological bacteria preparation is simple and convenient, and the cost is low. The prepared composite biological bacterium preparation can greatly improve the degradation rate of substances which are difficult to degrade and have toxicity.
Description
Technical Field
The invention relates to the technical field of biological environmental protection, in particular to a preparation method of a composite biological bacteria preparation suitable for sewage treatment.
Background
The water purifying agents used in the current sewage treatment process mainly comprise three types: inorganic water purifying agent, organic water purifying agent and microbial water purifying agent. Compared with microbial water purifying agents, the inorganic water purifying agents and the organic water purifying agents are widely used in sewage treatment at first, but the inorganic water purifying agents and the organic water purifying agents easily cause secondary pollution to the environment in the using process, and people are more and more careful in the process of treating sewage by using the inorganic water purifying agents and the organic water purifying agents in order to avoid the occurrence of secondary pollution to water quality. In contrast, more environmentally friendly microbial water purifiers are beginning to receive increasing attention.
The microbial water purifying agent in the prior art mainly comprises single biological flora and compound biological flora used as the microbial water purifying agent. However, the biological bacteria and the biological flora in the prior art still have the phenomenon of low degradation rate of substances which are difficult to degrade and have toxicity in the sewage in the process of being applied to sewage purification. In addition, the microbial water purifying agent prepared in the prior art has higher requirements on the survival conditions, the prepared biological agent can exert the water purifying effect only under the proper survival conditions, and the water purifying effect of the biological agent is reduced due to the problem that strains decline in the long-term use process of the biological agent.
Disclosure of Invention
In order to solve the problems, the invention discloses a preparation method of a composite biological bacteria preparation suitable for sewage treatment.
A composite biological bacteria preparation suitable for sewage treatment is prepared by the following steps:
(1) adding filtered purified water into a culture dish, performing primary aerobic treatment by adding micro aeration to the filtered purified water to obtain oxygen-enriched filtered purified water, adding a carbon source into the oxygen-enriched filtered purified water, stirring a mixture of the oxygen-enriched filtered purified water and the carbon source to completely dissolve the carbon source in the oxygen-enriched filtered purified water, and taking the oxygen-enriched filtered purified water with the completely dissolved carbon source as a culture solution.
(2) Adding a composite biological strain accounting for 1-2% of the culture solution by mass into the culture solution in the step (1), wherein the composite biological strain comprises: photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, bacillus, nitrobacteria, phosphorus solubilizing bacteria and nitrogen fixing bacteria.
(3) And (3) adding micro-aeration to the culture solution in the step (2) to carry out secondary aerobic treatment, maintaining the temperature of the culture solution at 30-40 ℃, and culturing the composite biological agent to obtain the required composite biological agent.
Further, the filtered purified water is filtered water obtained by filtering tap water by using an industrial water purifier, wherein the industrial water purifier can be an Agiler J2710-ROS126C model water purifier.
Further, the time of the first aerobic treatment in the step (1) is 12-20 hours, and the first aerobic treatment step is to ensure that the initial oxygen amount in the culture solution is sufficient.
Further, the time of the first aerobic treatment in the step (1) is 12 hours.
Furthermore, the carbon source is any one or a mixture of glucose, glycerol, sucrose and honey, and provides an energy source for strain propagation.
Further, the carbon source comprises litchi honey.
Further, the composite biological strain comprises the following biological bacteria in percentage by mass: 2-12% of photosynthetic bacteria, 4-10% of lactic acid bacteria, 4-15% of yeast, 3-13% of actinomycetes, 5-20% of bacillus, 4-15% of nitrobacteria, 4-15% of phosphorus-solubilizing bacteria and 8-15% of azotobacter.
Further, the temperature of the culture solution in the step (3) is maintained at 30-35 ℃, and the temperature can enable the compound biological strain to be rapidly propagated.
Further, the temperature of the culture solution in the step (3) was 32 ℃.
Further, the time of the second aerobic treatment in the step (3) is 14 to 16 days, and the second aerobic treatment can maintain the oxygen amount in the culture solution to be constant.
Further, the time of the second aerobic treatment in the step (3) is 15 days.
Further, the micro-aeration treatment step in the step (1) enables the dissolved oxygen content of the filtered and purified water to be kept above 5 mg/L.
Further, the micro-aeration treatment step in the step (3) keeps the dissolved oxygen content of the culture solution above 5 mg/L.
The compound biological bacteria preparation prepared by the invention comprises the following flora: photosynthetic flora, lactic acid flora, yeast flora, actinomycete flora, bacillus flora, nitrate flora, phosphate solubilizing flora, and nitrogen fixing flora.
The composite biological bacteria preparation prepared by the invention is applied to the sewage treatment process, and the composite biological bacteria prepared by the invention can be put in the sewage treatment process for multiple times.
Compared with the prior art, the invention achieves the following beneficial effects:
(1) the invention forms symbiotic flora by photosynthetic flora, lactic acid flora, yeast flora, actinomycete flora, bacillus flora, nitrate flora, phosphate solubilizing flora and nitrogen fixing flora through a compounding technology so as to symbiotically form a composite biological bacteria preparation, the composite biological bacteria preparation has the performance of the flora, and excellent sewage treatment performance is obtained through symbiotic culture in proper proportion; the composite biological bacteria preparation can be added into sewage to be fused with indigenous flora in the sewage to form an advantageous, optimized and strengthened biological activity system, the stability of the system is improved, meanwhile, the degradation rate of substances which are difficult to degrade and toxic can be greatly improved, and the treated sewage can be returned to the field for treatment.
(2) The composite biological bacterium preparation provided by the invention can be put in a sewage treatment process for multiple times, and can better solve the problem that the water purification effect is influenced by the decline of the use performance of biological bacteria in the use process.
(3) The preparation method of the composite biological agent is simple and convenient, has low cost, does not cause secondary pollution to water quality, and has the advantages of safety and environmental protection.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The preparation method of the composite biological bacteria preparation suitable for sewage treatment comprises the following steps:
(1) adding 500kg of filtered and purified water into a culture dish, adding micro aeration to the filtered and purified water to keep the dissolved oxygen content of the filtered and purified water above 5mg/L, carrying out primary aerobic treatment for 12 hours to obtain oxygen-enriched filtered and purified water, adding 10kg of litchi honey into the oxygen-enriched filtered and purified water, stirring a mixture of the oxygen-enriched filtered and purified water and the litchi honey to completely dissolve the litchi honey in the oxygen-enriched filtered and purified water, and taking the oxygen-enriched filtered and purified water in which the litchi honey is completely dissolved as a culture solution.
(2) 5kg of composite biological strain is added into the culture solution in the step (1), wherein the composite biological strain comprises 10 wt% of photosynthetic bacteria, 8 wt% of lactic acid bacteria, 13 wt% of yeast, 11 wt% of actinomycetes, 18 wt% of bacillus, 13 wt% of nitrobacteria, 13 wt% of phosphorus-solubilizing bacteria and 14 wt% of nitrogen-fixing bacteria.
(3) And (3) adding micro aeration to the culture solution in the step (2) to keep the dissolved oxygen content of the culture solution at more than 5mg/L, performing aerobic treatment for 15 days, maintaining the temperature of the culture solution at 32 ℃, and culturing the composite biological strain to obtain the composite biological bacterial preparation.
Examples of the experiments
1. The compound biological bacteria preparation prepared in the example 1 is used for treating pig-raising wastewater of Dongyuan Maofeng ecological agriculture development Limited company
A pretreatment stage: the liquid dung passing through the fence is 2000m3Injecting into an oxidation pond, aerating for 24 hours by using a water vehicle, and continuously aerating for three days.
The first stage treatment: and (3) adding 200kg of the composite biological bacteria preparation into the sewage of the oxidation pond, aerating for 10 hours every day, and continuously aerating for 15 days. Observing the state change of the sewage in the oxidation pond, the odor of the sewage in the oxidation pond disappears gradually after 3-6 hours of treatment, and the color of the pond water is changed from black to dark red along with the continuous oxygen exposure, and then is gradually changed from the dark red to transparent.
And (3) second-stage treatment: pumping the water on the upper layer of the oxidation pond, filtering the water through a 4-layer 400-mesh nylon screen, injecting the water into 3 brick-concrete ponds, wherein the capacity of each brick-concrete pond is 200T, adding a composite biological bacteria preparation (300g/T) for 24 hours, and continuously aerating for 3 days.
The water in each stage was submitted for examination, and the results are shown in table 1:
TABLE 1 Experimental example 1 Water quality test results at each stage
2. The compound biological bacteria preparation prepared in example 1 is used for treating wastewater of Meimegalane livestock farm in Ruian City
A pretreatment stage: the liquid dung passing through the fence is 2000m3Injecting into an oxidation pond, aerating for 24 hours by using a water vehicle, and continuously aerating for three days.
The first stage treatment: and (3) adding 200kg of the composite biological bacteria preparation into the sewage of the oxidation pond, aerating for 10 hours every day, and continuously aerating for 15 days. Observing the state change of the sewage in the oxidation pond, the odor of the sewage in the oxidation pond disappears gradually after 3-6 hours of treatment, and the color of the pond water is changed from black to dark red along with the continuous oxygen exposure, and then is changed from dark red to light yellow and is gradually transparent.
And (3) second-stage treatment: pumping the water on the upper layer of the oxidation pond, filtering the water through a 4-layer 400-mesh nylon screen, injecting the water into 3 brick-concrete ponds, wherein the capacity of each brick-concrete pond is 200T, adding a composite biological bacteria preparation (300g/T) for 24 hours, and continuously aerating for 3 days.
The water in each stage was submitted for examination, and the results are shown in table 2:
table 2 Experimental example 2 Water quality test results at each stage
Comparative example 1
The difference compared to example 1 is that the biological strain added in step (2) does not comprise Bacillus.
Comparative example
1. The biological bacterium preparation prepared in the comparative example 1 is used for treating pig-raising wastewater of Dongyuan Maofeng ecological agriculture development Limited company
A pretreatment stage: the liquid dung passing through the fence is 2000m3Injecting into an oxidation pond, aerating for 24 hours by using a water vehicle, and continuously aerating for three days.
The first stage treatment: and (3) adding 200kg of the composite biological bacteria preparation into the sewage of the oxidation pond, aerating for 10 hours every day, and continuously aerating for 15 days.
And (3) second-stage treatment: pumping the water on the upper layer of the oxidation pond, filtering the water through a 4-layer 400-mesh nylon screen, injecting the water into 3 brick-concrete ponds, wherein the capacity of each brick-concrete pond is 200T, adding a composite biological bacteria preparation (300g/T) for 24 hours, and continuously aerating for 3 days.
The water in each stage was submitted for examination, and the results are shown in table 3:
table 3 comparative experiment example 1 Water quality test results at respective stages
2. The biological bacteria preparation prepared in comparative example 1 is used for treating wastewater of Meimegalane livestock farm in Yuean City
A pretreatment stage: the liquid dung passing through the fence is 2000m3Injecting into an oxidation pond, aerating for 24 hours by using a water vehicle, and continuously aerating for three days.
The first stage treatment: and (3) adding 200kg of the composite biological bacteria preparation into the sewage of the oxidation pond, aerating for 10 hours every day, and continuously aerating for 15 days.
And (3) second-stage treatment: pumping the water on the upper layer of the oxidation pond, filtering the water through a 4-layer 400-mesh nylon screen, injecting the water into 3 brick-concrete ponds, wherein the capacity of each brick-concrete pond is 200T, adding a composite biological bacteria preparation (300g/T) for 24 hours, and continuously aerating for 3 days.
The water in each stage was submitted for examination, and the results are shown in table 4:
table 4 comparative experiment example 2 Water quality test results at various stages
In conclusion, the invention adopts a compounding technology to form symbiotic floras by photosynthetic floras, lactic acid floras, yeast floras, actinomycetes, bacillus floras, nitric acid floras, phosphorus-dissolving floras and nitrogen-fixing floras so as to symbiotically form the composite biological bacteria preparation, and the composite biological bacteria preparation has the performance of obtaining the floras and obtains excellent sewage treatment performance by symbiotic culture with proper proportion. According to test data, the composite biological bacterium preparation provided by the invention is used for treating sewage, the numerical values of indexes such as suspended substances, chemical oxygen demand, five-day biochemical oxygen demand, ammonia nitrogen, total phosphorus and the like in the treated sewage are greatly reduced compared with untreated sewage, the sewage is gradually changed from micro odor to odorless in the sewage treatment process, and the number of faecal escherichia coli groups in the sewage is obviously reduced.
The present invention has been further described with reference to specific embodiments, but it should be understood that the detailed description should not be construed as limiting the spirit and scope of the present invention, and various modifications made to the above-described embodiments by those of ordinary skill in the art after reading this specification are within the scope of the present invention.
Claims (10)
1. A preparation method of a composite biological bacteria preparation suitable for sewage treatment is characterized by comprising the following steps:
(1) taking filtered purified water and adding micro aeration for carrying out aerobic treatment for the first time to obtain oxygen-enriched filtered purified water; adding a carbon source into the oxygen-enriched filtered purified water, and uniformly mixing to obtain a culture solution for later use;
(2) adding a composite biological strain accounting for 1-2% of the culture solution in the step (1), wherein the composite biological strain comprises a mixture of photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes, bacillus, nitrobacteria, phosphorus solubilizing bacteria and nitrogen fixing bacteria;
(3) and adding micro-aeration into the culture solution added with the composite biological strain to perform secondary aerobic treatment, and culturing the composite biological strain to obtain the composite biological preparation.
2. The method for preparing a composite biological bacteria preparation suitable for sewage treatment according to claim 1, wherein in the step (1), the time of the first aerobic treatment step is 12-20 hours.
3. The method for preparing a composite biological bacteria preparation suitable for sewage treatment according to claim 2, wherein in the step (1), the carbon source comprises any one or a mixture of glucose, glycerol, sucrose and honey.
4. The method for preparing a composite biological bacteria preparation suitable for sewage treatment according to claim 3, wherein the carbon source comprises litchi honey.
5. The method for preparing a composite biological bacteria preparation suitable for sewage treatment according to claim 4, wherein in the step (2), the composite biological bacteria strain comprises the following biological bacteria in percentage by mass: 2-12% of photosynthetic bacteria, 4-10% of lactic acid bacteria, 4-15% of yeast, 3-13% of actinomycetes, 5-20% of bacillus, 4-15% of nitrobacteria, 4-15% of phosphate solubilizing bacteria and 8-15% of azotobacter.
6. The method for preparing a composite biological bacterial preparation suitable for sewage treatment according to claim 5, wherein in the step (3), the culture temperature of the composite biological bacterial preparation is controlled to be 25-30 ℃.
7. The method for preparing a composite biological bacteria preparation suitable for sewage treatment according to claim 6, wherein in the step (3), the time of the second aerobic treatment step is 14-16 days.
8. The method for preparing a composite biological bacteria preparation suitable for sewage treatment according to claim 7,
in the micro-aeration step in the step (1), the dissolved oxygen content of the filtered and purified water is kept above 5 mg/L;
in the step (3), the micro-aeration step keeps the dissolved oxygen content of the culture solution above 5 mg/L.
9. The composite biological bacterial preparation for sewage treatment prepared by the method of claim 8, which is characterized by comprising the following bacterial groups: photosynthetic flora, lactic acid flora, yeast flora, actinomycete flora, bacillus flora, nitrate flora, phosphate solubilizing flora, and nitrogen fixing flora.
10. The use of the complex biological bacterial preparation of claim 9 in the field of sewage treatment.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN101240238A (en) * | 2007-02-09 | 2008-08-13 | 维至康控股股份有限公司 | Method for manufacturing composition microorganism preparation |
| CN101503664A (en) * | 2009-03-12 | 2009-08-12 | 沈阳惠泽生物制品有限公司 | Method for producing efficient composite microbial preparation by livestock and poultry liquid dung |
| CN103589672A (en) * | 2013-11-15 | 2014-02-19 | 海南迈田生物科技有限公司 | Method for preparing effective microorganism water quality purification agent |
| CN104087530A (en) * | 2014-06-30 | 2014-10-08 | 嘉兴汇顺纺织染整有限公司 | Preparation process of flocculant for treating dyeing wastewater and application method of flocculant |
| CN105600945A (en) * | 2016-01-29 | 2016-05-25 | 南京神克隆科技有限公司 | Method for treating sewage by utilizing compound bacteria |
-
2019
- 2019-11-08 CN CN201911086377.2A patent/CN111004739A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1340364A (en) * | 1970-01-13 | 1973-12-12 | Hefermehl Sa H P | Method of adjusting the temperature in a biological breakdown |
| CN101240238A (en) * | 2007-02-09 | 2008-08-13 | 维至康控股股份有限公司 | Method for manufacturing composition microorganism preparation |
| CN101503664A (en) * | 2009-03-12 | 2009-08-12 | 沈阳惠泽生物制品有限公司 | Method for producing efficient composite microbial preparation by livestock and poultry liquid dung |
| CN103589672A (en) * | 2013-11-15 | 2014-02-19 | 海南迈田生物科技有限公司 | Method for preparing effective microorganism water quality purification agent |
| CN104087530A (en) * | 2014-06-30 | 2014-10-08 | 嘉兴汇顺纺织染整有限公司 | Preparation process of flocculant for treating dyeing wastewater and application method of flocculant |
| CN105600945A (en) * | 2016-01-29 | 2016-05-25 | 南京神克隆科技有限公司 | Method for treating sewage by utilizing compound bacteria |
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