CN109402016A - For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method - Google Patents
For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method Download PDFInfo
- Publication number
- CN109402016A CN109402016A CN201811404309.1A CN201811404309A CN109402016A CN 109402016 A CN109402016 A CN 109402016A CN 201811404309 A CN201811404309 A CN 201811404309A CN 109402016 A CN109402016 A CN 109402016A
- Authority
- CN
- China
- Prior art keywords
- pseudomonas
- bacillus
- micro organism
- strain
- organism fungicide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention will be unfolded for the requirement of functional flora needed for one stable flora of the common pollutant in current chemical industry environmental protection field and building, by using the means of enrichment selection culture and large scale fermentation, the composite functional bacterial agent that can be suitable for general chemical industry biochemical treatment of wastewater synergy is prepared.The microorganism of the one of which forecast scheme configuration composite bacteria agent of the invention mainly includes 108 kinds of microorganism fungus kinds of three zones type.By adding complex micro organism fungicide and the carrier for promoting microorganism to grow or nutritional agents etc. in biochemical system, promote the foundation of the complete food chain of biochemical system using a variety of strains with specific pollutant degradation capability in complex microorganism preparations and complete metabolic way, promote utilization and metabolic breakdown of the microorganism to Pollutants in Wastewater matter, realizes the efficient reduction of COD, ammonia nitrogen, p and s etc..
Description
Technical field
The invention belongs to environment protection field, in particular to wastewater from chemical industry biological treatments.
Background technique
Biological wastewater treatment technology is the leading and core technology of current field of waste water treatment, it is as a kind of economic, conjunction
Reason, effective wastewater processing technology, in current city, field of industrial waste water treatment of having stable political situation plays important function.Biological wastewater treatment
Technology is the metabolism using nature microorganism, and the organic matter in decomposition and inversion waste water reaches the mesh of purification of water quality
's.Nutriment needed for microorganism obtains energy and cell synthesis from whole process.The core of biological wastewater treatment technology
Be culture and tame be suitble to particular types waste water superior microorganism flora, and keep with Pollutants in Wastewater concentration be adapted
Microbial biomass and activity make superior microorganism flora be in optimal degrading activity, give full play to its degradation efficiency, to greatest extent
Raising biological treatment system processing capacity and treatment effeciency.The degradation of the degradation effect of Pollutants in Wastewater and entire flora
It is related that can function spectrum meet pollutant kind in sewage.The core of biological wastewater treatment technology is microorganism in processing system
Mass transfer effect the two key factors in group and reactor.Wherein, microbial population is by initial inoculation in processing system
Microbe species and reactor for treatment during introduce or proliferation microbe species constitute.Microbial flora is biological anti-
The maximum factor of Function difference in device is answered, the treatment effect for improving reactor has very big exploit potentialities.
Wastewater from chemical industry is the key points and difficulties of current biological wastewater treatment, and key is in waste water containing many difficult to degrade
There is high toxic material with to microorganism.High hardly degraded organic substance in waste water is generally referred to containing phenols, alkanes, aromatic hydrocarbon
Class, cyanogen class and amine etc..This type organic general structure is stablized, and is insoluble in water, high poison, difficult to degrade, and is easy in biology
Enrichment in vivo has three to cause effect.Contained at this stage with the microbiological treatment that traditional activated sludge culture obtains above-mentioned
Often will appear in the waste water of organic matter bad adaptability, cultivation cycle are long, degradation capability is poor, out water unstable the problems such as, be difficult to reach
To sewage drainage standard.Generally for this kind of height organic wastewater difficult to degrade is coped with, frequently with physicochemical techniques or dilution means
Pre-treatment is carried out to it.The difficulty of the processing cost and operation operation that lead to waste water in this way is significantly greatly increased.In addition also have using strong
The biological treatment of change, such as in bioreactor fill carrier or improved using ultrafiltration membrane the method for sludge concentration come
Cope with this kind of intractable waste water.Such methods are by improving activated sludge concentration come the degradation energy for biological treatment system of competing
Power, but thing-microorganism fungus kind without fundamentally changing the most essential core of biological treatment system.To in biological treatment system
The flora in portion optimize or even construct new microflora can fundamentally improve bioreactor degradation efficiency and
Degradation capability.For the complex micro organism fungicide of these pollutant particular formulations difficult to degrade, may alleviate well above-mentioned
Situation can reach preferable treatment effect to above-mentioned this kind of organic wastewater.
Complex micro organism fungicide is the high efficient strain for different hardly degraded organic substances directed screening from natural environment,
By orientation domestication culture, the dominant microflora degraded to certain organic efficient is formed, will be obtained after these dominant microfloras compounding
's.The activity and quantity of microorganism can be improved in the microbial bacterial agent being configured in this way, accelerates the degradation to organic pollutant.
Occur the patented technology and product much in terms of building compound bacteria is applied to environmental improvement both at home and abroad at present,
In there are many specifically in the composite bacteria of a certain specific type waste water or pollutant.Such as application No. is
200810154236.5, in the patent of entitled " for handling the microorganism formulation and production method of landfill leachate ", propose
It is prepared using the strain compound criteria of five seed type such as Bacillus cercus, photosynthetic bacteria, nitrifier, denitrifying bacterium and saccharomycete
Microbial inoculum is added in the biochemical treatment system of landfill leachate, to improve the removal effect of COD and ammonia nitrogen in percolate with high salt.Again
Such as application No. is 201010278691.3, entitled " a method of utilize complex microorganism preparations to handle waste water ", the patent
It proposes that putting into initial concentration in biochemistry pool is the complex micro organism fungicide of biological tank processing volume 0.15-0.3%, and puts into 1-
Carrier of the diatomite or Powdered Activated Carbon of 2% pool capacity as microorganism fixation, wherein complex microorganism preparations are by acetic acid
Common microorganism is constituted in more than the 100 kinds of sewage such as acetobacter, liquefaction acetobacter, and this method can establish complete microorganism
Food chain improves the thorough degradability of various pollutants.For another example number of patent application is 200410005196.X, and title is " for locating
Manage the microorganism formulation and preparation method thereof of highly difficult waste water ", propose to separate cultivation and fermentation using anaerobic and aerobic, then press than
Efficient complex micro organism fungicide is prepared after example mixing, microorganism therein includes acetic acid acetobacter, liquefaction acetobacter, the wooden vinegar
Bacillus etc. 47 belongs to 105 kinds of microorganisms.The microorganism formulation can be used for handling coking wastewater, gourmet powder waste water, antibiotic waste water etc.
Waste water difficult to deal with can obtain preferable treatment effect in conjunction with Powdered Activated Carbon is added as carrier.
Although occurring the numerous microorganism formulation of value volume and range of product both at home and abroad at present for field of environmental improvement, wherein having specially
Door also has the compound bacteria for being suitable for wide spectrum functional water waste for Mr. Yu's class waste water or the microbial compound preparation of pollutant.But mesh
Composite bacteria agent involved in preceding patent both domestic and external, all without going out multiple functions degradation bacteria for its group for pollutant programmed screening
It applies altogether, in the preparation of preparation and compound bacteria, does not also account for and how to allow the spontaneous flocculation of composite bacteria agent at zoogloea
To prevent from being lost.
, mainly there is following problem in current microbial bacterial agent both domestic and external:
1. many complex micro organism fungicides at this stage in activated sludge mainly after screening and taming, by strain mixture
It is applied to sewage treatment later.Although the complex micro organism fungicide obtained in this way is preferable for the treatment effect of a few class sewage,
But since screening face is narrow, so causing degradable high hardly degraded organic substance type in microbial inoculum few.And at this stage in sewage
The type of organic matter is more complicated, so the practicability is poor, and treatment effect is bad with such composite bacteria agent in processing.Preparation
Compound bacteria out is limited on degradation capability and the range of application.
2. it is excellent to be only absorbed in its for used compound bacteria in the document and Patent Application Publication delivered both at home and abroad at present
Gesture growth ability or degradation are easy to the ability of biochemical organic matter, without overall thinking construct one it is stable, be easy to
The composite flora of itself fixed growth, the compound bacteria flocculation sedimentation and film forming ability of building are poor, it is necessary to be carried out using carrier
It is fixed, cause the cost of investment of compound bacteria engineer application to increase.
3. training method used by most of complex micro organism fungicide is relatively simple now, mainly Anaerobic culturel and
Aerobic culture.Such as application No. is 20041005196.X, entitled " for handling the microorganism formulation and its system of highly difficult waste water
The patent of Preparation Method ", what is used is exactly that aerobic and Anaerobic culturel is respectively adopted, then will be after culture in 105 kinds of bacterial strains after mixed
Strain in Anaerobic culturel strain: it is aerobic culture strain be 1:3 ratio mix.Such training method is although relatively simple,
But problem is also obvious.Since the quantity of strain in composite bacteria agent is more, the growth characteristics of strain are also different, certain growths
Faster bacterial strain can inhibit some generations for growing slower bacterial strain after forming dominant microflora, so as to cause the dense of certain bacterial strains
It spends and expected effect is not achieved down to degradation.
4. conventional composite bacteria agent is applied to the water environment treatment or single-minded waste water aspect of slight pollution more at present, and
Can adapt to without one kind for high wastewater from chemical industry aspect difficult to degrade and highly toxic, cause the application range of high-effective service by
To limitation.
In conclusion lacking one kind being capable of the common object difficult to degrade of comprehensive degradation at present in chemical wastewater treatment field
Matter and the composite bacteria agent for forming stable compound microecological system.
Summary of the invention
In order to solve the deficiency of above-mentioned existing various methods, the method for the present invention will be common for current chemical industry environmental protection field
The requirement of functional flora needed for one stable flora of pollutant and building is unfolded, by using enrichment selection culture and on a large scale
The means of fermentation prepare the composite functional bacterial agent that can be suitable for general chemical industry biochemical treatment of wastewater synergy.By
Complex micro organism fungicide and the carrier for promoting microorganism to grow or nutritional agents etc. are added in biochemical system, utilize complex microorganism
A variety of strains with specific pollutant degradation capability and complete metabolic way promote the complete food of biochemical system in preparation
The foundation of chain promotes utilization and metabolic breakdown of the microorganism to Pollutants in Wastewater matter, realizes the height of COD, ammonia nitrogen, p and s etc.
Effect is cut down.
One of the object of the invention is to provide a kind of complex micro organism fungicide for chemical wastewater treatment.
It is a further object of the present invention to provide the Constructed wetlands and preparation method of a kind of microorganism formulation.
According to an aspect of the present invention, a kind of complex micro organism fungicide for chemical wastewater treatment is provided, containing not
The three categories microorganism fungus kind of congenerous, three categories function strain are respectively the flora of object directed screening difficult to degrade, sewage treatment
Basic flora and building complex ecosystem functional flora.
It is according to the present invention to provide a kind of complex micro organism fungicide for chemical wastewater treatment there are also on the one hand, it is multiple
Closing microbial bacterial agent also includes stabilizer and strain protective agent.
The method that the present invention uses is first three classes functional flora required for one complex micro organism fungicide of determining building: needle
To functional flora needed for the flora of object directed screening difficult to degrade, sewage treatment basis flora and building complex ecosystem.
The microorganism of one of forecast scheme configuration composite bacteria agent mainly includes 108 kinds of microorganism fungus kinds of three zones type.
First kind function is the microbial flora of common contaminant of capable of degrading, and the method for preparation is by general
Pollutant to be degraded is added in minimal medium, is cultivated by multiple enrichment and selectivity, filtering out has efficiently drop
The strain of the pollutant is solved, then is carried out purifying preservation and identification.The microbial flora of first kind function mainly includes as follows
(it is corresponding degradation product title that the left side of the table, which is on the right side of strain name) 65 kinds of microorganism fungus kinds, have the corresponding pollution of degradation in table
The function of object.
The flora of object directed screening difficult to degrade
The second class function that composite bacteria agent has is to strengthen the degradation of nitrogen phosphorus, biomembrane attachment, conventional substances conversion and waste water
The sludge systems reinforcing functions such as decoloration, the autotrophic bacterium of nitrogen phosphate and sulfur therein conversion be by prepare special autotrophy culture medium come
Selection enrichment, and other some degradations or Decolourization Bacteria are enriched with by the selectivity culture of orientation.
The microbial flora of second class function mainly includes following 28 kinds of microorganism fungus kinds, is turned with corresponding substance
Change or specific function.
Construct complex ecosystem functional flora
The third class function bacterium that composite bacteria agent has has the function of dominant growth and high adaptation chemical wastewater treatment, wherein
Strain be to be obtained by the culture and enrichment of selectivity from the common chemical wastewater biochemical processing system sludge.Third
The microbial flora of class function mainly includes following 15 kinds of microorganism fungus kinds.
Be above microorganism formulation is utilized in the present invention microorganism fungus kind title and its during application from
Effect.
From strain screens in industrial wastewater in complex micro organism fungicide for chemical wastewater treatment of the invention, work
Industry waste water include waste water from dyestuff, pigment wastewater, breeding wastewater, cotton pulp wastewater, acid waste water, industrial wastewater containing phenol, fine logical sequence waste water,
It is one or more in low temperature municipal wastewater, Fine Chemistry Wastewater, agricultural chemicals waste water, antibiotic waste water and coking wastewater.
The complex micro organism fungicide bacterial screening method for chemical wastewater treatment provided is provided, is from will be from all kinds of
After purification through strain separating, screening three categories function strain is respectively hardly possible for the activated sludge of wastewater from chemical industry and contaminated muddy water sample
The flora of degradation product directed screening, sewage treatment often use flora and building complex ecosystem functional flora.
A kind of preparation of complex micro organism fungicide for chemical wastewater treatment is additionally provided according to an aspect of the present invention
Method, comprising the following steps:
1) 108 kinds of bacterial strains described in claim 3 to 5 are individually cultivated first, with selective minimal medium
Prepare seed liquor.
2) cultured seed liquor is divided into five groups by its fermenting property, the seed liquor of this five groups of mixing is inoculated into respectively
Fermentor, inoculum concentration be 5%~10%, 30~35 DEG C of cultivation temperature, the initial ph value 6.0~8.0 of fermentation medium.
A kind of preparation of complex micro organism fungicide for chemical wastewater treatment is additionally provided according to an aspect of the present invention
Cultured seed liquor is divided into five groups by its fermenting property by method, this method, group basis are as follows:
, according to the life style and the speed of growth of strain, anaerobism will be divided into during Anaerobic culturel and aerobic culture
Mixed culture group, aerobic fast-growth group, aerobic middling speed culture group, aerobic culture group at a slow speed；
Aerobic bacteria culture uses mechanical stirring using logical filtrated air, Anaerobic culturel in fermentation process；Wherein anaerobism group
Incubation time is 48 hours；Three groups aerobic of incubation time is followed successively by 24 hours, 48 hours and 72 hours.
A kind of preparation of complex micro organism fungicide for chemical wastewater treatment is additionally provided according to an aspect of the present invention
Method, anaerobism group use glucose complete medium, and cultivation temperature is 30-35 DEG C, and the method stirred using anaerobism is stirred per hour
It mixes 2 times, every time 10 minutes；It is aerobic to use two kinds of culture mediums, respectively bacterium complete medium and glucose complete medium.
A kind of preparation of complex micro organism fungicide for chemical wastewater treatment is additionally provided according to an aspect of the present invention
Method, bacterium complete medium are beef-protein medium.
The present invention is described below from 108 kinds of microorganisms of above-mentioned reference from screening the microorganism fungus kind for preparing product
Method and steps used by production process.
To screen the flora in the present invention with degradation common contaminant function, the method and experimental procedure used is such as
Under:
1. original flora source: acquisition by such contaminants water sample perhaps mud sample or with the pollutant preparation
Artificial wastewater tame common Municipal activated sludge, obtain the microbial mixture as bacterium source.
2. original enriching floras: preparing liquid LB enriched medium, be inoculated with a small amount of sludge, 25-30 DEG C of temperature, originate pH
7-8, shaking speed 120r/min cultivate 2-5d, obtain the mixed microorganism rich in target degradation function.Enriched medium is by having
A certain amount of pollutant adds diluted bacterium often to be formed with culture medium.
3. selectivity domestication culture: preparing using the drug of the Single Pollution object of low concentration as carbon source or nitrogen source, supplement phase
The selective medium of the nitrogen source or carbon source and inorganic salts answered is inoculated with the 10% original flora being enriched with through step 2, in temperature
25-30 DEG C, pH 7-8,2-7d is cultivated, becomes muddy to selective medium, then obtained culture is inoculated into 10% amount
In fresh selective medium, the concentration of pollutant is gradually incremented by fresh selective medium, tame three periods with
On, obtain the liquid culture high-efficient for this contaminant degradation.
4. strain separating purifies:
It separates the preparation of plate: preparing selected liq culture medium described in 3, add the agar powder of 1.5-2%, sterilize
Afterwards, selective plate is made in inverted plate.
Strain separating: liquid culture obtained in 3 is subjected to gradient dilution with physiological saline, by the liquid after dilution
It is applied on selective plate, 2-7d is cultivated at a temperature of 25-30 DEG C.
Purifying agaric: selecting single bacterium colony from the plate of above-mentioned culture, with isolated method LB culture medium again of crossing
It is carried out in plate.Repetition is crossed 2-3 times, and the single culture until being separated to and under the microscope observation carry out observation determination and be but
Pure culture.This method can separate the degradation function group of different compounds, can also be separated to the dominant growth in waste water
Strain.
5. the measurement of strain degradation ability: the strain that will be isolated and purified is inoculated into the inorganic salts of the pollutant containing a certain concentration
In culture solution, 25-30 DEG C of temperature, pH 7-8, shaking speed 120r/min are originated, cultivates 7d, degradation results are observed in timing.Screening
One kind last to such organic pollutant degradation effect or two kinds of bacterial strains out.
The identification of 6 bacterial strains: by it is separated to the method that is combined using morphologic observation and Physiology and biochemistry of bacterial strain, according to document
Carry out CHARACTERISTICS IDENTIFICATION.The colonial morphology for the bacterial strain being separated to, the morphosis of thallus are observed, biochemical reactions include that leather is blue
Albert'stain Albert, flagella staining, capsule stain, glucose fermentation, lactose fermentation, catalase experiment, oxidizing ferment measurement experiment,
Starch Hydrolysis experiment, methyl red experiment, hydrogen sulfide experiment, indoles experiment etc..
7. fermentation condition optimization: obtained bacterial strain optimizes its condition of culture.Condition of culture includes: starting pH
Value, shaking speed, temperature, dissolved oxygen amount, inoculum concentration etc..
8. the function strain of identification is inoculated into fermentation medium with concentration of the same race, mixed fermentation expands, and identifies hair
Various strains are not lost after ferment.
To obtain the heretofore described autotrophic bacterium with inorganic elements transformation function, it is somebody's turn to do using preparing to contain
The autotrophy culture medium of object obtains the function strain by enrichment culture.
The present invention is from 108 kinds of Pure cultured spawns of preservation to the process institute that spreads cultivation of the microorganism formulation containing 108 kinds of strains
The method and steps of use is as follows:
1. the metabolic type of 108 kinds of microorganisms is determined to the bio-chemical characteristics of purebred strain according to previous experiments, by it
It is divided into heterotrophism metabolism group and chemautotrophy metabolism group.According to its physiological metabolism feature, by heterotrophism metabolic components at anaerobism (simultaneous)
Oxygen group and aerobic group.In addition, aerobic group is divided into quickly further according to the period measurement data bred to growth early period are tested
Growth group, middling speed growth group and slow growth group.
2. the mode that spreads cultivation of chemoautotrophic bacteria are as follows: using the minimal medium containing the element prepared, be free of other
It constantly spreads cultivation under conditions of organic matter or chaff interferent.As nitrifier spreads cultivation using the nothing containing ammonium sulfate and sodium nitrite
Machine salt culture medium obtains the culture containing nitrifier by constantly amplifying culture.By constantly expanding culture, obtain
Bacteria culture fluid needed in proportion, is added in final mix bacterium agent product by equal proportion.
3. heterotrophism metabolism group includes anaerobism group and aerobic group, heterotrophism metabolism bacterium is to obtain with decomposing organic matter and therefrom and grow
What required energy and small-molecule substance was characterized.Spreading cultivation for heterotrophism group bacterium is divided into two steps, the preparation of seed liquor before this, so
After be to mix seed liquor to spread cultivation.It is the basis to spread cultivation that the preparation of seed liquor, which is with the selective medium of the strain,
(with glucose replacement if without specific candidate) guarantees that the function of strain is not lost by the culture of selectivity.
In the selective incubation of certain seed liquor, it should according to the physiological metabolism feature of strain, selection anaerobism stirring or good
The mode of oxygen is cultivated.After seed liquor is spread cultivation by the culture medium of selectivity, guarantee its volume as that can provide for mixed culture
The seed liquor of 5-10%.
4. detesting the mode that spreads cultivation of (simultaneous) oxygen group in heterotrophism metabolism group are as follows: the single prepared is detested to the kind of (simultaneous) oxygen animalcule
Sub- liquid mixing is based on glucose complete medium, and the mode of oxygen stirring, by fermentation in 48 hours, detests to complete mixing
Oxygen bacterium spreads cultivation.25~30 DEG C of cultivation temperature, the initial ph value 6.0~8.0 of fermentation medium, under conditions of sterile and anoxic
It is completed through everfermentation.
5. aerobic group in heterotrophism group, further according to the speed of its speed of growth in bacterium complete medium, being divided into fast
Long group of fast-growing, middling speed growth group and slow growth group.The aerobic group of strain that heterotrophism is metabolized all individually is trained with Selective agar medium
Feeding mode spreads cultivation out seed liquor.Then it is divided into three above-mentioned groups further according to its speed of growth speed.Divide the strain at one group
Seed liquor mix, obtain the seed liquor of three groups of mixing.By this three groups of seed liquors respectively in glucose complete medium
With filtrated air culture is passed through in bacterium complete medium.Its time cultivated is respectively 24 according to three groups of the different speeds of growth
Hour, 48 hours and 72 hours.After the completion of culture, three groups of mixed aerobic bacteria kinds that two kinds of different culture mediums spread cultivation are obtained.
6. increasing to 10 to strain density after completing autotrophic bacterium group and anaerobic bacteria, respectively the spreading cultivation of aerobic bacteria8Afterwards further according to every
The strain ratio occupied in group is by the tunning to spread cultivation autotrophic bacterium group in proportion: anaerobic bacteria=6:4 is mixed, and bacterium is added
Kind protective agent and stabilizer, to obtain the microorganism formulation in the present invention.
Strain protective agent and stabilizer are a kind of or a few class in Tween-20, xanthan gum and glycerol.
Compared to current patent document and previous high efficiency composition object microorganism formulation and preparation method thereof, in the present invention
Microorganism formulation and preparation method thereof has the advantage that
1. having degradation is a variety of to have been generally acknowledged that chemical pollution object difficult to degrade, complex microorganism bacterial classification provided in the present invention
In a major class function bacterium be all with target contaminant prepare selective medium in screen, to target contaminant
With very strong single-minded degradation property.Therefore, pass through its pollutant with wide spectrum of the complex micro organism fungicide of this method preparation
Degradation property has the potentiality for being widely used in chemical wastewater treatment field.
2. there are a variety of miscellaneous functions, including the multiple functions such as the oxidation of denitrogenation dephosphorizing, sulphur, flocculation film forming and waste water decoloring,
This is because the composite bacteria in the present invention has most microorganism fungus kind to have the second class function, it is mainly compound
Strain provides miscellaneous function.It can guarantee that the multiple-effect functionality of strain can be together with flocculation sedimentation with it in this way, or have
Under conditions of carrier, strengthen the biofilm formation growth ability of compound bacteria using film forming bacterium.
3. having the adaptability grown in all kinds of chemical wastewater treatments to have due to the microorganism formulation in the present invention
A part is the dominant bacteria filtered out from a variety of wastewater from chemical industry, is present in strain preparation after everfermentation, is being added to
When in similar wastewater from chemical industry, the characteristic with dominant growth.
4. it is above-mentioned can preferably to guarantee that the strain preparation after spreading cultivation contains using the method for grouping in strain spreads cultivation
108 kinds of microorganism fungus kinds, autotrophic bacterium and heterotroph are separately cultivated, and are conducive to the growth and proliferation of autotrophic bacterium.It is spreading cultivation simultaneously
During, the become reconciled strain of oxygen metabolism of anaerobic metabolism is separated, can guarantee two kinds of strains the case where being suitble to spontaneous condition
Lower growth, will not be inhibited by outside environmental elements.Furthermore the present invention also innovate by for the most aerobic bacteria groups of number be divided into it is fast,
In, it is three groups slow, be able to solve the risk that strain is squeezed out at a slow speed in different speed of growth strains mixed culture, can preferably protect
Demonstrate,prove spreading cultivation for the strain of slow growth.
5. it is separately cultivated in the spreading cultivation of strain using seed liquor, it is then multiple to spread cultivation in the mode for the fermentation that is mixed
Combined bacteria kind.Seed liquor can guarantee the pure and strain function of seed liquor using the culture medium and sterile culture condition of selectivity
Stability.After completing seed liquor preparation, then seed liquor is mixed in proportion, mixed fermentation goes out microculture
Object.It is fermented, can preferably be guaranteed in final microbial inoculum containing big using the method that seed liquor and two step of mixed fermentation separate
Partial original strain.
6. being different from common composite bacteria preparation, microorganism formulation provided by the invention only need to be added disposably, after adding
Strain can be flocculated with itself at zoogloea, or in the event it is supported, strong film-forming bacterium can preferentially attach to grow on carrier,
It guides other strains to be attached on carrier, quickly forms biomembrane.
The concentration of every kind of bacterium requires to reach 108Meet the requirements.
Complex micro organism fungicide disclosed in this invention for chemical wastewater treatment can be widely applied to handle common
Wastewater from chemical industry, such as coking wastewater, pharmacy waste water, antibiotic waste water, pigment wastewater, dyeing waste water, agricultural chemicals waste water, flavors and fragrances
Adding for the microbial bacterial agent can be combined from different biochemical processing process, can be passed through by waste water, oil wastewater etc.
The new artificial microecosystem of one kind is constructed to strengthen purification of waste water.
Detailed description of the invention
Fig. 1 is the function classification figure of complex micro organism fungicide disclosed in this invention；
Fig. 2 is the preparation flow figure of the composite bacteria agent disclosed in this invention for chemical wastewater treatment.
Specific embodiment
Embodiment 1: screening, preparation are used for the complex micro organism fungicide of chemical wastewater treatment, and process is as shown in Figure 1
Screening purification process can be carried out according to content in foregoing specification, and details are not described herein again.
By isolating and purifying from nature or artificial environment, screening obtains 108 kinds of pure cultures of above-mentioned three zones
Microorganism fungus kind, the principle of screening are that the function of strain as needed prepares selective medium or provides other selectivity
Condition to make the strain fast breeding needed, and inhibits unwanted growth.The selective medium master prepared when screening
If being formulated by microorganism basic inorganic salt culture medium plus target contaminant, the formula of basic inorganic salt culture medium is such as
Under: potassium dihydrogen phosphate: 1.0g, dipotassium hydrogen phosphate: 2.6g, magnesium sulfate 0.2g, ammonium chloride: 0.2g, sodium chloride: 0.25g, yeast are taken out
Extract:
0.1g, deionized water: 1000ml, PH 7.4-7.6
Function strain is filtered out by the way that the culture environment of target degradation product or glucose creation selectivity is added, is passed through
After the screening of the present inventor, the function strain of above-mentioned three categories is obtained, amounts to 108 kinds.Be purified again culture after it is stored refrigerated,
Original strain as complex micro organism fungicide.
As shown in Figure 2
It completes bacterial screening and with postponing, is starting the step that spreads cultivation for carrying out strain.The physiological metabolism feature of strain is first pressed,
Flora is divided into autotrophy group and heterotrophism group, the inorganic matter that autotrophy group is metabolized further according to microorganismization energy anabolism prepares it
Single-minded culture medium spreads cultivation.Main autotrophic bacterium is two class of Autotrophic nitrification bacterium and sulfur oxidizing bacterium.The side that its culture medium is prepared
Method is that the metabolism bottom as its specificity such as ammonium salt, nitrate and reducible sulfur compound is added in inorganic salts basal medium
Object.The concentration of the metabolism substrate of specificity is gradually increased in the screening of autotrophic bacterium, metabolism substrate can be prevented to bacterium in this way
It plants the toxicity of itself and screens the strain more resistant to high concentration substrate.The screening process of heterotroph is mainly concerned with bacterium source mud
The acquisition of (water) sample, the concentration of target strain, the screening of strain liquid culture medium, the separation of strain selectivity plate, strain it is pure
Change culture and etc..Its process screened, and according to metabolism substrate concentration is stepped up, metabolism is selected from isolated strain
Several plants of bacterium of fast speed get up as function bacterium preservation of the invention.Screen 108 plants of single bacteriums are fitted according to its growth
The environment answered prepares the independent seed liquors of 108 kinds of bacterium, while in the preparation process of seed liquor, culture medium selects its degradation
Target contaminant as its metabolism substrate, guarantee that the function of the strain in the preparation process of seed liquor is not lost.
It, will according to the environment of its growth and the speed of growth rate by 108 kinds of function bacteriums in the preparation for completing seed liquor
It is divided into autotrophy group, anaerobism group, quick group aerobic, aerobic middling speed group and aerobic group at a slow speed.Wherein autotrophy group is corresponding using its
Metabolism substrate and minimal medium are expanded, and anaerobism group then uses glucose complete medium, incubation time 48h；It is good
Oxygen group is fast, in two groups use bacterium complete medium, slow group uses glucose complete medium, incubation time be respectively for 24 hours,
48h, 72h.
The wherein component of beef-protein medium are as follows: 0.3 part of beef extract, 1.0 parts of peptone, 0.5 part of sodium chloride,
100 parts of distilled water, after mixing, adjusting pH is 7.0-7.2 to each component, in 1.05kg/cm2Lower sterilizing 22 minutes.
The component of glucose complete medium are as follows: 0.3 part of beef extract, 1.0 parts of peptone, 1.0 parts of glucose, distilled water
After mixing, adjusting pH is 7.0-7.2 to 100 parts of each components, in 1.05kg/cm2Lower sterilizing 22 minutes.
The culture of microorganism obtained after fermentation according to object directed screening difficult to degrade flora: sewage treatment basis flora:
Complex ecosystem functional flora 6:2:2 is constructed to be mixed, then in addition stabilizer and strain protective agent, it is qualified to examine
The complex micro organism fungicide disclosed in this invention for chemical wastewater treatment is obtained afterwards.The standard of inspection is with final production
108 kinds of strains containing above-mentioned three classes function can be verified in product.
Embodiment 2: complex micro organism fungicide is used for feed addictive wastewater treatment
The feed addictive for handling certain production glycine betaine and allicin using complex micro organism fungicide disclosed by the invention is useless
Water, the technique of processing are A/O+ sedimentation basin.The water quality situation of the production waste water are as follows: COD 1100-1200mg/L, TN 300-
400mg/L, NH3-N 60-70mg/L contain a large amount of glycine betaine and trimethylamine hydrochloride in waste water.Stop for A sections in treatment process
Stay the time 12 hours, O sections the residence time 36 hours.The composite filling for accounting for pond body dischargeable capacity 70% is installed in AO biochemistry pool
Adhere to carrier as strain.It is disposably added in AO biochemistry pool and accounts for the prepared by the present invention compound of pond body dischargeable capacity 2%
Microbial bacterial agent starts small flow and carries out amplification and strain biofilm, steps up feed water flow according to the biofilm situation of strain
Amount, until intaking at full capacity.By amplification in 20 days and acclimation and screening, khaki mycoderm is hung up on filler, completes the expansion of strain
Increase and biofilm, treatment effect are as follows:
Embodiment 3: complex micro organism fungicide is for antibiotic waste water processing
Ferment antibiotics Wastewater Pollutant concentration is high, water is big, contained composition be mainly fermentation residues, demulsifier and
Residual antibiotic potency and its degradation product, there are also various organic solvents remaining in antibiotic extraction process and some inorganic salts
Deng.Its waste water is complex in composition, functional leaves are out of proportion (nitrogen source is superfluous), contains the biochemistry such as a large amount of sulfate, titer of antibodies
Mortifier, toxic, waste water bio-refractory.Domestic antibiotic industry major part waste water treatment efficiency is not high at present, stablizes
Up to standard is less.
The sulfuric acid slime mould of certain enterprise is handled with the complex micro organism fungicide disclosed in this invention for chemical wastewater treatment
Plain waste water uses fixed bed membrane bioreactor for main body technology, before plus hydrolysis acidification technique.Process flow is as follows:
Antibiotic waste water → conditioning tank → hydrolysis acidification pool → fixed bed membrane bioreactor → coagulation air-float → water outlet
Wherein the compound for the treatment of tank body 2% is added according to there is the composite filling for accounting for basin's effective volume 70% in hydrolysis acidification pool
Microbial bacterial agent.Oxygen compatibility pool controls 20-35 DEG C of temperature, pH value 6-7, and dissolved oxygen is lower than 0.5mg/L, and sludge concentration 4-8g/L stops
Stay the time 24 hours, the sludge of sedimentation basin persistently flows back.
Filled with modified macrocellular foam carrier in fixed bed membrane bioreactor, adds and add the compound for the treatment of tank body 2%
Microbial bacterial agent.Fixed bed membrane bioreactor controls 20-35 DEG C of temperature, pH value 6-8, dissolved oxygen 2-4mg/L, sludge concentration
10-20g/L, the residence time 48 hours, air-flotation process after water outlet carries out coagulation with PAC and PAM, the body refuse of generation was directly discharged to
Sludge concentration tank.
It there is no suspended matter in colistin sulphate waste water raw water, by above-mentioned two biochemical process unit and compound
It is final to locate after debugging in 30 days after the processing of microbial inoculum, then with the method for coagulation air-float by the thallus to suspend in water outlet removal
It is as follows to manage result:
Embodiment 3: complex micro organism fungicide is used for Treatment of Coking Effluent
In the cooling procedure of coke and coking of coal gas and production ammonia, the process of tar, naphthalene, phenol and light oil is generated
A large amount of production waste water.Its water quality characteristic is basic are as follows: BOD5=1,000mg/L；COD=1,500-6,000mg/L；TSS=
200mg/L；Phenol=150-2,000mg/L；Benzene=10mg/L.In addition to this also contain certain density ammonia nitrogen, cyanide is more
Aromatic hydrocarbon ring quasi polymer (PAHs).The production waste water for being derived from coke-oven plant, Hebei province is tested.The water of this strand production waste water
Matter feature is as follows: COD=3,928-4,982mg/L；NH3-N=64-169mg/L；PH=7.5-8.5.The process flow of use
Are as follows:
Coking wastewater → conditioning tank → ABR → SBR → blowoff basin
The active carbon particle for accounting for pond body dischargeable capacity 4-5% is added as strain carrier in ABR.When effective stop of ABR
Between be 48 hours, using the concatenated unit of four lattice.ABR controls 32-36 DEG C of temperature, and pH value 7.5-8.5 is stirred using water distribution and reflux
It mixes.
The Powdered Activated Carbon for accounting for pond body dischargeable capacity 5-8% is added in SBR as strain carrier, effective stop of SBR
Time is 48h, using two pond body parallel runnings.SBR controls 20-30 DEG C of temperature, pH value 7.0-8.0, dissolved oxygen 2.0-
3.0mg/L.Sludge concentration 4-6g/L, settling ratio SV20-30%.By above-mentioned two biochemical process unit and composite bacteria agent
After processing, then with the method for coagulation air-float by the thallus to suspend in water outlet remove, after debugging in 30 days, final process result
It is as follows:
The above embodiments are some preferred embodiments in order to further illustrate the present invention, and not all embodiments.This
Field professional made under the premise of no progress creative work based on the other embodiment of the present invention, belong to this
The rights protection scope of invention.
Claims (11)
1. a kind of complex micro organism fungicide for chemical wastewater treatment, which is characterized in that the complex micro organism fungicide contains
The three categories microorganism fungus kind of different function, the three categories function strain are respectively the flora of object directed screening difficult to degrade, dirt
Water process basis flora and building complex ecosystem functional flora.
2. a kind of complex micro organism fungicide for chemical wastewater treatment according to claim 1, which is characterized in that described
Complex micro organism fungicide also includes stabilizer and strain protective agent.
3. a kind of complex micro organism fungicide for chemical wastewater treatment according to claim 1, which is characterized in that
The flora of the object directed screening difficult to degrade includes the function bacterium that following 65 kinds of hardly degraded organic substances decompose:
Pseudomonas putida (Pseudomonas putida)；
Acinetobacter baumannii (Acinetobacter aumannii)；
Thermophilic vinyl chloride Selective medium (Pseudonocardia loroethenivorans)；
Thermophilic pyridine Rhodococcus sp (Rhodococcus pyridinivorans)；
Acinetobacter baumannii (Acinetobacter aumannii)；
Meng Shi pseudomonad (Pseudomonas monteili)；
Pseudomonad (Pseudomonas fulva)；
Bacillus (Bacillus sp.)；
Pseudomonad (Pseudomonas fulva)；
Klebsiella (Klebsiella sp.)；
Micrococcus luteus (Micrococcus sp.)
Pseudomonad (Pseudomonas sp.)；
Chlorophenol arthrobacterium (Arthrobacter chlorophenolicus)；
Chlorophenol Sphingol single-cell (Sphingonas chlorophenolica)；
From hair monad (Comamonas sp.)；
Microbacterium (Microbacterium sp.)；
Anthropi (Ochrobactrum tritici)；
The yellow flat lead fungi of spore (Phanerochaete chrysosporium)；
Microbacterium (Microbacterium sp.)；
Acidophilic bacteria (Delftia acidovorans)；
Pseudomonas putida (Pseudomonas sp.)；
Klebsiella (Klebsiella variicola)；
Staphylococcus (Staphylococcus sp.)；
Flavobacterium (Flavobacterium sp.)；
Arthrobacterium (Arthrobacter sp.)；
Anthropi (Ochrobactrum tritici)；
Pseudomonad (Pseudomonas sp.)；
Bacillus flavus (Xanthobacter flavus)；
Sphingolipid bacterium (Sphingobium yanoikuyae)；
Novosphingobium sp.；
Starkeya sp.；
Halomonas (Halomonas sp.)
Glaciecola chathamensis；
Si Shi pseudomonas (Pseudomonas stutzeri)；
Comamonas testosteroni (Comamonas testosteroni)；
Pseudomonas putida (Pseudomonas putida)；
Pseudomonas aeruginosa (Pseudomonas aeruginosa)；
Gordonia bronchialis (Gordonia sp.)；
Bacillus (Bacillus sp.)；
Clostridium sporogenes (Clostridium sporogenes)；
Thermophilic dioxane Selective medium (Pseudonocardia dioxanivorans)；
Coral Nocard's bacillus (Nocardia cordllina)；
Flavobacterium (Flavobacterium sp.)；
Pseudomonas testosteroni (Pseudomonas estosteroni)；
Micrococcus luteus (Micrococcus sp.)；
Cellulomonas cartae (Celluromonas sp.)；
Pseudomonas mendocina (Pseudomonas mendocina)；
Human pallid bacillus (Ochrobactrum anthropi)；
Germ oligotrophy unit cell (Stenotrophomonas maltophilia)；
Bacillus megatherium (Bacillus megaterium)；
Pseudomonas aeruginosa (Pseudomonas aeruginosa)；
Acinetobacter calcoaceticus (Acinetobacter sp.)；
Methyl parathion degradation bacterium (Alcaligenes sp.)；
Stenotrophomonas (Stenotrophomonas acidaminiphila)；
Pseudomonas alcaligenes (Pseudomonas alcaligense)；
Sphingol single-cell (Sphingobium sp.)；
Aeromonas (Aeromonas sp.)；
Anthropi (Ochrobactrum sp.)；
Bacillus (Bacillus sp.)；
Pseudomonad (Pseudomonas sp)；
Selective medium (Pseudonocardia sulfidoxydans)；
The colourless subspecies of aeromonas salmonicida (Aeromonas salmonicicla subsp.)；
Achromobacter (Achromobacter sp.)；
Enterobacteria (Enterobacter sp.)；
Escherichia (Escherichia sp.).
4. a kind of complex micro organism fungicide for chemical wastewater treatment according to claim 1, which is characterized in that described
Constructing complex ecosystem functional flora includes that following 28 kinds of biosystem functions supplement strain:
It nitrifies pseudomonad (Pseuomonas nitroreducens)；
Bacterium nitrobacter (Nitrobacter winogradskyi)；
Anthropi (Ochrobactrum anthropi)；
Flavobacterium mizutaii；
Denitrification richness salt bacterium (Haloferax denitrificans)；
Denitrification achromobacter (Achromobacter denitrificans)；
Alcaligenes dentrificans (Alcaligenes faecalis)；
Pseudomonas stutzeri (Pseudomonas stutzeri)；
Pseudomonas (Pseudomonas sp.)；
Rhodopseudomonas palustris (Rhodopsedudomonas palustris)；
Rhodospirillum rubrum (Rhodospirillum rubrum)；
The red spirillum of salt (Halorhodospira sp.)；
Thiobacillus (Starkeya sp.)；
Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans)；
Aquatic abnormal cocci (Deinococcus aquaticus)；
Bacillus licheniformis (Bacillus licheniformis)；
It solves fiber bacillus (Bacillus cellulosilyticus)；
Ammoxidation bacillus flavus (Xanthobacter aminoxidans)；
Burkholderia (Burkholderia cepacia)；
Pod membrane Rhodococcus sp (Rhodobacter capsulata)；
Syncephalastrum racemosum；
Pseudomonas fluorescens (Pseudomonas fluorescens)；
Acinetobacter calcoaceticus (Acinetobacter sp.)；
Shewanella decolorationis (Shewanella decolorationis)；
Wax printing fabric (Bacillus cereus)；
Pseudozyma rugulosa；
Bacillus (Bacillus sp.)；
Pseudomonad (Pseudomonas sp.).
5. a kind of complex micro organism fungicide for chemical wastewater treatment according to claim 1, which is characterized in that described
Sewage treatment basis flora includes the following 15 kinds microorganism fungus kinds screened from current common industrial biological treatment of waste water system:
Actinomyces (Actinomycetes sp.)；
The red spirillum of Mohs (Phaeospirillum molischianum)；
Pseudomonas fluorescens (Pseudomonas fluorescens)；
It is bent bacillus (Bacillus flexus)；
Bacillus wakoensis；
Secondary coccus (Paracoccus sp.)；
Bacillus alcalophilus (Bacillus halodurans)；
Issatchenkia orientalis (Issatchenkia orientails)；
Pseudomonas aeruginosa (Pseudomonas aeruginosa)；
Coral Nocard's bacillus (Nocardia coralline)；
Salmon color Nocard's bacillus (Nocardia salmonicolor)；
Cold-resistant planococcus (Planococcus psychrotoleratus)；
Pseudomonad (Pseudomonas sp.)；
Simple pimelobacter sp (Pimelobacter simplex)；
It solves rouge sea bacillus (Marinobactor lipolyticus).
6. a kind of complex micro organism fungicide for chemical wastewater treatment according to claim 1, which is characterized in that described
From being screened in industrial wastewater for strain in the complex micro organism fungicide of chemical wastewater treatment, the industrial wastewater includes dye
Expect that waste water, pigment wastewater, breeding wastewater, cotton pulp wastewater, acid waste water, industrial wastewater containing phenol, fine logical sequence waste water, low temperature municipal administration are dirty
It is one or more in water, Fine Chemistry Wastewater, agricultural chemicals waste water, antibiotic waste water and coking wastewater.
7. a kind of complex micro organism fungicide bacterial screening method for chemical wastewater treatment, which is characterized in that from will be from all kinds of
After purification through strain separating, screening three categories function strain is respectively hardly possible for the activated sludge of wastewater from chemical industry and contaminated muddy water sample
Flora, sewage treatment basis flora and the building complex ecosystem functional flora of degradation product directed screening.
8. a kind of preparation method of the complex micro organism fungicide for chemical wastewater treatment, which comprises the following steps:
1) 108 kinds of bacterial strains described in claim 3 to 5 are individually cultivated first, is prepared with selective minimal medium
Seed liquor.
2) cultured seed liquor is divided into five groups by its fermenting property, the seed liquor of this five groups of mixing is inoculated into fermentation respectively
Tank, inoculum concentration be 5%~10%, 30~35 DEG C of cultivation temperature, the initial ph value 6.0~8.0 of fermentation medium.
9. a kind of preparation method of the complex micro organism fungicide for chemical wastewater treatment as described in claim 8, special
Sign is, described that cultured seed liquor is divided into five groups by its fermenting property, group basis are as follows:
, according to the life style and the speed of growth of strain, anaerobism mixing will be divided into during Anaerobic culturel and aerobic culture
Culture group, aerobic fast-growth group, aerobic middling speed culture group, aerobic culture group at a slow speed；
Aerobic bacteria culture uses mechanical stirring using logical filtrated air, Anaerobic culturel in fermentation process；The wherein culture of anaerobism group
Time is 48 hours；Three groups aerobic of incubation time is followed successively by 24 hours, 48 hours and 72 hours.
10. a kind of preparation method of the complex micro organism fungicide for chemical wastewater treatment as described in claim 9, special
Sign is that anaerobism group uses glucose complete medium, and cultivation temperature is 30-35 DEG C, the method stirred using anaerobism, per hour
Stirring 2 times, every time 10 minutes；It is aerobic to use two kinds of culture mediums, respectively bacterium complete medium and glucose complete medium.
11. a kind of preparation method of complex micro organism fungicide for chemical wastewater treatment as described in claim 10,
It is characterized in that, bacterium complete medium is beef-protein medium.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811118430 | 2018-09-21 | ||
| CN2018111184308 | 2018-09-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109402016A true CN109402016A (en) | 2019-03-01 |
Family
ID=65474806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811404309.1A Pending CN109402016A (en) | 2018-09-21 | 2018-11-23 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109402016A (en) |
Cited By (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109456906A (en) * | 2018-12-27 | 2019-03-12 | 黄河三角洲京博化工研究院有限公司 | A kind of alcohol-containing type organic sewage dedicated processes microbial inoculum and processing method |
| CN110028166A (en) * | 2019-03-12 | 2019-07-19 | 宜兴国际环保城科技发展有限公司 | A kind of novel C OD remover |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
| CN110294533A (en) * | 2019-06-25 | 2019-10-01 | 鞍钢股份有限公司 | A kind of continuous biological reinforced technique of suitable processing coking wastewater |
| CN110438041A (en) * | 2019-07-22 | 2019-11-12 | 常德职业技术学院 | A kind of Novel compound microbial agent for Treatment of Sludge |
| CN110452858A (en) * | 2019-09-12 | 2019-11-15 | 中国科学院微生物研究所 | One Paracoccus B54 and its application |
| CN110468081A (en) * | 2019-09-17 | 2019-11-19 | 湖南艾布鲁环保科技股份有限公司 | Composite bacteria agent and its preparation method and application |
| CN110683656A (en) * | 2019-09-12 | 2020-01-14 | 绍兴海坤水处理技术有限公司 | Method for treating aniline printing and dyeing wastewater by using microbial agent |
| CN110699313A (en) * | 2019-10-29 | 2020-01-17 | 天津市工业微生物研究所有限公司 | Screening and culturing method of specific microbial flora for degrading industrial wastewater |
| CN110734886A (en) * | 2019-12-11 | 2020-01-31 | 山东科技大学 | Bacterial strain capable of tolerating high-concentration chromium and application thereof |
| CN110734877A (en) * | 2019-11-11 | 2020-01-31 | 上海骄阳环境工程有限公司 | strain polycyclic aromatic hydrocarbon high-efficiency degrading bacterium and application thereof |
| CN110862942A (en) * | 2019-11-22 | 2020-03-06 | 辽宁科技大学 | Stenotrophomonas and application thereof |
| CN110964687A (en) * | 2019-12-23 | 2020-04-07 | 天津市工业微生物研究所有限公司 | Preparation method of compound microbial agent for wastewater treatment |
| CN110980962A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Phosphorus-accumulating microbial inoculum and sludge treatment technology using same |
| CN111187742A (en) * | 2020-02-26 | 2020-05-22 | 上海淳渊环境科技有限公司 | Composite biological agent and method and system for treating salt-containing organic matter wastewater |
| CN111296487A (en) * | 2020-02-12 | 2020-06-19 | 黑龙江八一农垦大学 | Method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings |
| CN111307736A (en) * | 2020-02-10 | 2020-06-19 | 天津大学 | Method for evaluating influence of industrial park wastewater on biochemical sewage treatment |
| CN111661929A (en) * | 2020-06-05 | 2020-09-15 | 北京赛富威环境工程技术有限公司 | Organic waste treatment method |
| CN111718864A (en) * | 2020-01-21 | 2020-09-29 | 武汉工程大学 | Rare earth leaching site soil indigenous high-efficiency denitrification strain pseudomonas flaviviridis K3 and pseudomonas morganii K17 and application thereof |
| CN111748509A (en) * | 2020-07-03 | 2020-10-09 | 浙江工商大学 | Microbial purifying preparation for seawater culture tail water, and preparation method and application thereof |
| CN111909873A (en) * | 2020-08-11 | 2020-11-10 | 大连理工大学 | Mixed bacteria with polycyclic aromatic hydrocarbon degradation capability and screening method and application thereof |
| CN111924977A (en) * | 2020-07-14 | 2020-11-13 | 海南微能科技有限公司 | Method for treating nitrate nitrogen wastewater by using composite flora |
| CN112063568A (en) * | 2020-09-30 | 2020-12-11 | 天津大学 | Heterotrophic nitrification-aerobic denitrification bacterial agent and application thereof in natural water purification |
| CN112094772A (en) * | 2020-09-09 | 2020-12-18 | 上海上药杏灵科技药业股份有限公司 | Biological agent and preparation method and application thereof |
| CN112174337A (en) * | 2020-09-28 | 2021-01-05 | 西南大学 | Application of kitchen waste fermentation liquor in sewage treatment |
| CN113151077A (en) * | 2021-04-14 | 2021-07-23 | 广西大学 | Pseudomonas aeruginosa and screening method and application thereof |
| CN113233614A (en) * | 2021-03-13 | 2021-08-10 | 徐韡卿 | Composite functional microbial inoculum for aerobic culture biological method on-line regeneration of activated carbon adsorption carriers and application thereof |
| CN113354208A (en) * | 2021-06-18 | 2021-09-07 | 无锡普力环保科技有限公司 | Wastewater zero discharge process |
| CN113444661A (en) * | 2021-06-21 | 2021-09-28 | 温州大学 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
| CN113667615A (en) * | 2020-12-22 | 2021-11-19 | 南京工程学院 | Azo dye degrading bacterium and application thereof |
| CN113755386A (en) * | 2021-09-30 | 2021-12-07 | 清华大学天津高端装备研究院 | Composite microbial inoculum for treating high-COD cutting fluid waste liquid and process method |
| CN113817636A (en) * | 2021-09-22 | 2021-12-21 | 哈尔滨工业大学 | High-temperature-resistant nitrifying bacteria for reducing ammonia emission in sludge composting process and application thereof |
| TWI766554B (en) * | 2021-01-22 | 2022-06-01 | 財團法人食品工業發展研究所 | Microbial deodorizing reagent, method for deodorizing compost and use of mixed strains |
| CN114684917A (en) * | 2020-12-31 | 2022-07-01 | 南通醋酸化工股份有限公司 | Method for treating sorbic acid wastewater and dehydroacetic acid comprehensive wastewater |
| CN114908002A (en) * | 2022-04-14 | 2022-08-16 | 华南理工大学 | Biological nano-selenium reinforced composite strain and application thereof |
| CN114958624A (en) * | 2022-06-29 | 2022-08-30 | 济宁市农业科学研究院 | Phanerochaete chrysosporium JX1 and screening method and application thereof |
| CN115197881A (en) * | 2022-07-22 | 2022-10-18 | 苏州鑫泷生态环境有限公司 | Compound microbial agent for photovoltaic wastewater treatment and preparation method and application thereof |
| CN115259397A (en) * | 2022-06-30 | 2022-11-01 | 苏州鑫泷生态环境有限公司 | Composite strain for treating aquaculture wastewater |
| KR20230031804A (en) * | 2021-08-27 | 2023-03-07 | 재단법인 농축산용미생물산업육성지원센터 | Composition for decomposing pesticides |
| CN115838665A (en) * | 2022-11-15 | 2023-03-24 | 中国科学院城市环境研究所 | Enterobacter strain TCD1-1 T And uses thereof |
| CN116040816A (en) * | 2022-12-21 | 2023-05-02 | 中国科学院天津工业生物技术研究所 | Microbial agent hydrogel and application thereof in heparin sodium wastewater treatment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
| CN104195090A (en) * | 2014-09-12 | 2014-12-10 | 范鸿仁 | Compound microbiological preparation and application thereof in waste water treatment |
| CN107916242A (en) * | 2017-12-29 | 2018-04-17 | 南昌工程学院 | A kind of peasant household's sewage disposal composite bacteria agent and preparation method thereof |
| CN108118008A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application |
-
2018
- 2018-11-23 CN CN201811404309.1A patent/CN109402016A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
| CN104195090A (en) * | 2014-09-12 | 2014-12-10 | 范鸿仁 | Compound microbiological preparation and application thereof in waste water treatment |
| CN108118008A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application |
| CN107916242A (en) * | 2017-12-29 | 2018-04-17 | 南昌工程学院 | A kind of peasant household's sewage disposal composite bacteria agent and preparation method thereof |
Cited By (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109456906A (en) * | 2018-12-27 | 2019-03-12 | 黄河三角洲京博化工研究院有限公司 | A kind of alcohol-containing type organic sewage dedicated processes microbial inoculum and processing method |
| CN110028166A (en) * | 2019-03-12 | 2019-07-19 | 宜兴国际环保城科技发展有限公司 | A kind of novel C OD remover |
| CN110156174B (en) * | 2019-05-30 | 2022-01-25 | 杭州秀川科技有限公司 | Mixed strain fermentation biological pretreatment method for high-concentration high-salt pharmaceutical wastewater |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
| CN110294533A (en) * | 2019-06-25 | 2019-10-01 | 鞍钢股份有限公司 | A kind of continuous biological reinforced technique of suitable processing coking wastewater |
| CN110438041A (en) * | 2019-07-22 | 2019-11-12 | 常德职业技术学院 | A kind of Novel compound microbial agent for Treatment of Sludge |
| CN110452858A (en) * | 2019-09-12 | 2019-11-15 | 中国科学院微生物研究所 | One Paracoccus B54 and its application |
| CN110683656A (en) * | 2019-09-12 | 2020-01-14 | 绍兴海坤水处理技术有限公司 | Method for treating aniline printing and dyeing wastewater by using microbial agent |
| CN110452858B (en) * | 2019-09-12 | 2021-04-27 | 中国科学院微生物研究所 | Paracoccus B54 and application thereof |
| CN110468081A (en) * | 2019-09-17 | 2019-11-19 | 湖南艾布鲁环保科技股份有限公司 | Composite bacteria agent and its preparation method and application |
| CN110699313A (en) * | 2019-10-29 | 2020-01-17 | 天津市工业微生物研究所有限公司 | Screening and culturing method of specific microbial flora for degrading industrial wastewater |
| CN110734877B (en) * | 2019-11-11 | 2022-09-16 | 上海骄阳环境工程有限公司 | Polycyclic aromatic hydrocarbon efficient degrading bacterium and application thereof |
| CN110734877A (en) * | 2019-11-11 | 2020-01-31 | 上海骄阳环境工程有限公司 | strain polycyclic aromatic hydrocarbon high-efficiency degrading bacterium and application thereof |
| CN110862942B (en) * | 2019-11-22 | 2023-02-03 | 辽宁科技大学 | Stenotrophomonas and application thereof |
| CN110862942A (en) * | 2019-11-22 | 2020-03-06 | 辽宁科技大学 | Stenotrophomonas and application thereof |
| CN110980962A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Phosphorus-accumulating microbial inoculum and sludge treatment technology using same |
| CN110734886A (en) * | 2019-12-11 | 2020-01-31 | 山东科技大学 | Bacterial strain capable of tolerating high-concentration chromium and application thereof |
| CN110734886B (en) * | 2019-12-11 | 2021-06-04 | 山东科技大学 | Bacterial strain capable of tolerating high-concentration chromium and application thereof |
| CN110964687A (en) * | 2019-12-23 | 2020-04-07 | 天津市工业微生物研究所有限公司 | Preparation method of compound microbial agent for wastewater treatment |
| CN111718864B (en) * | 2020-01-21 | 2022-05-10 | 武汉工程大学 | Rare earth leaching site soil indigenous high-efficiency denitrification strain pseudomonas flaviviridis K3 and pseudomonas morganii K17 and application thereof |
| CN111718864A (en) * | 2020-01-21 | 2020-09-29 | 武汉工程大学 | Rare earth leaching site soil indigenous high-efficiency denitrification strain pseudomonas flaviviridis K3 and pseudomonas morganii K17 and application thereof |
| CN111307736A (en) * | 2020-02-10 | 2020-06-19 | 天津大学 | Method for evaluating influence of industrial park wastewater on biochemical sewage treatment |
| CN111296487B (en) * | 2020-02-12 | 2021-06-29 | 黑龙江八一农垦大学 | Method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings |
| CN111296487A (en) * | 2020-02-12 | 2020-06-19 | 黑龙江八一农垦大学 | Method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings |
| CN111187742A (en) * | 2020-02-26 | 2020-05-22 | 上海淳渊环境科技有限公司 | Composite biological agent and method and system for treating salt-containing organic matter wastewater |
| CN111661929A (en) * | 2020-06-05 | 2020-09-15 | 北京赛富威环境工程技术有限公司 | Organic waste treatment method |
| CN111661929B (en) * | 2020-06-05 | 2021-08-24 | 北京赛富威环境工程技术有限公司 | Organic waste treatment method |
| CN111748509A (en) * | 2020-07-03 | 2020-10-09 | 浙江工商大学 | Microbial purifying preparation for seawater culture tail water, and preparation method and application thereof |
| CN111924977A (en) * | 2020-07-14 | 2020-11-13 | 海南微能科技有限公司 | Method for treating nitrate nitrogen wastewater by using composite flora |
| CN111909873A (en) * | 2020-08-11 | 2020-11-10 | 大连理工大学 | Mixed bacteria with polycyclic aromatic hydrocarbon degradation capability and screening method and application thereof |
| CN111909873B (en) * | 2020-08-11 | 2022-05-31 | 大连理工大学 | Mixed bacteria with polycyclic aromatic hydrocarbon degradation capability and screening method and application thereof |
| CN112094772A (en) * | 2020-09-09 | 2020-12-18 | 上海上药杏灵科技药业股份有限公司 | Biological agent and preparation method and application thereof |
| CN112174337B (en) * | 2020-09-28 | 2022-09-13 | 西南大学 | Application of kitchen waste fermentation liquor in sewage treatment |
| CN112174337A (en) * | 2020-09-28 | 2021-01-05 | 西南大学 | Application of kitchen waste fermentation liquor in sewage treatment |
| CN112063568A (en) * | 2020-09-30 | 2020-12-11 | 天津大学 | Heterotrophic nitrification-aerobic denitrification bacterial agent and application thereof in natural water purification |
| CN112063568B (en) * | 2020-09-30 | 2023-01-03 | 天津大学 | Heterotrophic nitrification-aerobic denitrification bacterial agent and application thereof in natural water purification |
| CN113667615B (en) * | 2020-12-22 | 2023-05-16 | 南京工程学院 | Azo dye degrading bacterium and application thereof |
| CN113667615A (en) * | 2020-12-22 | 2021-11-19 | 南京工程学院 | Azo dye degrading bacterium and application thereof |
| CN114684917B (en) * | 2020-12-31 | 2023-09-15 | 南通醋酸化工股份有限公司 | Method for treating sorbic acid wastewater and dehydroacetic acid comprehensive wastewater |
| CN114684917A (en) * | 2020-12-31 | 2022-07-01 | 南通醋酸化工股份有限公司 | Method for treating sorbic acid wastewater and dehydroacetic acid comprehensive wastewater |
| TWI766554B (en) * | 2021-01-22 | 2022-06-01 | 財團法人食品工業發展研究所 | Microbial deodorizing reagent, method for deodorizing compost and use of mixed strains |
| CN113233614A (en) * | 2021-03-13 | 2021-08-10 | 徐韡卿 | Composite functional microbial inoculum for aerobic culture biological method on-line regeneration of activated carbon adsorption carriers and application thereof |
| CN113151077A (en) * | 2021-04-14 | 2021-07-23 | 广西大学 | Pseudomonas aeruginosa and screening method and application thereof |
| CN113151077B (en) * | 2021-04-14 | 2023-03-21 | 广西大学 | Pseudomonas aeruginosa and screening method and application thereof |
| CN113354208A (en) * | 2021-06-18 | 2021-09-07 | 无锡普力环保科技有限公司 | Wastewater zero discharge process |
| CN113444661B (en) * | 2021-06-21 | 2022-03-04 | 温州大学 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
| CN113444661A (en) * | 2021-06-21 | 2021-09-28 | 温州大学 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
| KR20230031804A (en) * | 2021-08-27 | 2023-03-07 | 재단법인 농축산용미생물산업육성지원센터 | Composition for decomposing pesticides |
| KR102520249B1 (en) | 2021-08-27 | 2023-04-11 | 재단법인 농축산용미생물산업육성지원센터 | Composition for decomposing pesticides |
| CN113817636B (en) * | 2021-09-22 | 2023-07-07 | 哈尔滨工业大学 | High-temperature-resistant nitrifying bacteria capable of reducing ammonia emission in sludge composting process and application thereof |
| CN113817636A (en) * | 2021-09-22 | 2021-12-21 | 哈尔滨工业大学 | High-temperature-resistant nitrifying bacteria for reducing ammonia emission in sludge composting process and application thereof |
| CN113755386B (en) * | 2021-09-30 | 2023-12-26 | 清华大学天津高端装备研究院 | Composite microbial agent for treating high COD cutting fluid waste liquid and technological method |
| CN113755386A (en) * | 2021-09-30 | 2021-12-07 | 清华大学天津高端装备研究院 | Composite microbial inoculum for treating high-COD cutting fluid waste liquid and process method |
| CN114908002A (en) * | 2022-04-14 | 2022-08-16 | 华南理工大学 | Biological nano-selenium reinforced composite strain and application thereof |
| CN114958624A (en) * | 2022-06-29 | 2022-08-30 | 济宁市农业科学研究院 | Phanerochaete chrysosporium JX1 and screening method and application thereof |
| CN114958624B (en) * | 2022-06-29 | 2024-01-02 | 济宁市农业科学研究院 | Phanerochaete chrysosporium JX1 and screening method and application thereof |
| CN115259397A (en) * | 2022-06-30 | 2022-11-01 | 苏州鑫泷生态环境有限公司 | Composite strain for treating aquaculture wastewater |
| CN115197881A (en) * | 2022-07-22 | 2022-10-18 | 苏州鑫泷生态环境有限公司 | Compound microbial agent for photovoltaic wastewater treatment and preparation method and application thereof |
| CN115838665A (en) * | 2022-11-15 | 2023-03-24 | 中国科学院城市环境研究所 | Enterobacter strain TCD1-1 T And uses thereof |
| CN115838665B (en) * | 2022-11-15 | 2024-05-31 | 中国科学院城市环境研究所 | Enterobacter strain TCD1-1TAnd applications thereof |
| CN116040816A (en) * | 2022-12-21 | 2023-05-02 | 中国科学院天津工业生物技术研究所 | Microbial agent hydrogel and application thereof in heparin sodium wastewater treatment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109402016A (en) | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method | |
| Xie et al. | Biological sulfamethoxazole degradation along with anaerobically digested centrate treatment by immobilized microalgal-bacterial consortium: performance, mechanism and shifts in bacterial and microalgal communities | |
| Shen et al. | Municipal wastewater treatment via co-immobilized microalgal-bacterial symbiosis: Microorganism growth and nutrients removal | |
| CN108102955B (en) | The effectively compound microbial inoculant and preparation method thereof administered for black and odorous water | |
| Zheng et al. | Substrates removal and growth kinetic characteristics of a heterotrophic nitrifying-aerobic denitrifying bacterium, Acinetobacter harbinensis HITLi7T at 2 C | |
| Lu et al. | Identification and nitrogen removal characteristics of Thauera sp. FDN-01 and application in sequencing batch biofilm reactor | |
| CN102465103B (en) | Aerobic denitrification methylobacterium phyllosphaerae and application thereof | |
| CN102976497B (en) | Method for treating high-concentration organic waste water by bacilli | |
| Saravanane et al. | Bioaugmentation and treatment of cephalexin drug-based pharmaceutical effluent in an upflow anaerobic fluidized bed system | |
| CN102465105B (en) | Nitrous acid-type denitrification bacteria strain and application thereof | |
| CN108977399A (en) | One plant of Bacillus foecalis alkaligenes and its application | |
| CN112391308A (en) | Compound microbial agent for treating high-ammonia-nitrogen sewage and preparation method thereof | |
| CN102190373B (en) | Aerobic and facultative anaerobic microbial activated bactericide for water treatment and application thereof in contact oxidation process | |
| Ma et al. | Cooperation triggers nitrogen removal and algal inhibition by actinomycetes during landscape water treatment: performance and metabolic activity | |
| CN106927576B (en) | Method for improving removal effect of nitrogen pollutants in sewage | |
| CN103642712B (en) | A kind of composite flora for paper waste of degrading and preparation method thereof | |
| CN102373191B (en) | Dominant consortium flora for degrading urban waste water and preparation method thereof | |
| CN108342338B (en) | Method for treating pharmaceutical wastewater containing antibiotics | |
| CN111793565A (en) | ABS microbial agent for sewage treatment and preparation method thereof | |
| CN103627657A (en) | Composite microflora for degrading lignin wastewater and preparation method thereof | |
| CN107244746B (en) | Pyridine and phenol degrading bacteria and application thereof in treatment of wastewater containing pyridine and phenol | |
| KR100237972B1 (en) | Liquid microbe disposal agent for disposing of waste water | |
| CN109576184A (en) | A kind of complex microbial inoculum for waste water of basic printing and dyeing processing | |
| KR101558945B1 (en) | A Novel Microorganism with Superior Petroleum Oil Degradation Activity | |
| Franco-Rivera et al. | Characterization and performance of constructed nitrifying biofilms during nitrogen bioremediation of a wastewater effluent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |