CN104195090A - Compound microbiological preparation and application thereof in waste water treatment - Google Patents
Compound microbiological preparation and application thereof in waste water treatment Download PDFInfo
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- CN104195090A CN104195090A CN201410464776.9A CN201410464776A CN104195090A CN 104195090 A CN104195090 A CN 104195090A CN 201410464776 A CN201410464776 A CN 201410464776A CN 104195090 A CN104195090 A CN 104195090A
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Abstract
The invention discloses a compound microbiological preparation and application thereof in the waste water treatment. Strains adopted by the invention can be conveniently obtained from a natural world by technicians in the field according to the conventional strain identifying and separating method, or can be publicly purchased by a commercial channel. All culture solutions and microbial carriers are mixed according to a certain proportion to obtain the compound microbiological preparation. The compound microbiological preparation disclosed by the invention contains 38 strains; the compound microbiological preparation can be directly fed in a biochemical reactor at one time, so that the establishment of a complete food chain in a microbial system can be effectively promoted, the utilization of microorganisms for nutrient substances in water is promoted, and high-efficiency removal of polluted substances such as organic pollutants (such as benzene ring halogenated compounds), ammonia, nitrogen and the like is realized. The compound microbiological preparation is suitable for biochemical treatment of waste water which is high in salt content, contains heterocyclic aromatic hydrocarbons and is difficult to degrade.
Description
Invention field
The present invention relates to a kind of complex microorganism preparations and the application in wastewater treatment thereof.
Background of invention
Along with the development of human social economy, some synthesis of organic substance constantly produce, and many chemical industry products can not directly have been degraded by single microorganism.For this reason, many scholars have launched a large amount of research.For a long time, to the organic pollutant of some difficult degradations (as phenyl ring halogenated compound) people, still can not be separated to them as the high-performance bio degrading microorganism of sole carbon source and the energy.Some researchs show, as the pollutant of trieline can only carry out biological degradation by common metabolism.McCarty has found two groups of bacteriums, can under methanogenic anaerobic condition, grow and degrade trieline and zellon for one group, another group bacterium can utilize the metabolism growth matrix (being called for short altogether for thing) altogether such as phenol and dimethylbenzene, the trieline of degrading under the condition that has air to exist.For this type organic, utilizing the common metabolism of microorganism to degrade is an effective approach.Our company is according to administering for many years the experience of trade effluent, for trade effluent is poisonous, harmful, organic concentration is high, the feature that is difficult to biochemical treatment developed microbial preparation of the present invention.
Wastewater from chemical industry all has certain biocidal property, and the pollutent even having cannot be degraded by single Institute of Micro-biology, and all biochemical systems strictly do not screen microbe species in the microbial inoculant phase substantially.To such an extent as to many biochemical systems are because the disappearance of indivedual bacterial classifications in system cannot form good common metabolic reaction.So conventionally all can there is degradation efficiency step-down in daily administration, the problem that system impact resistance is poor.Because always thinking in water treatment field in the past, the strain domestication of wastewater treatment, only need to, according to the principle of the survival of the fittest in natural selection, must have a collection of bacterium being survived, these bacteriums often all conform, and even have the ability of good degraded water pollutant.In fact true really not so simple, the spore of justing think often has many bifurcateds, and the dominant bacteria of placing hope on self-assembling formation is in fact very pessimistic.And often system because lose some bacterium, and certain poisonous substance in cannot water of decomposition, to such an extent as to other bacterial classifications also do not have good just forming to be eliminated by environmental poisonous substance, the bacterial classification that certainly also has some to survive and, but often because self population quantity deficiency and formation that cannot be a large amount of.
Complex microorganism preparations of the present invention is through screening for many years, a kind of efficient, the non-secondary pollution biotechnological formulation of domestication in practical engineering application.For complex industrial waste water, select the formulated biotechnological formulation of multiple bacterial classification, object is to allow different bacterial classifications assist mutually in biochemical system complicated pollutent is formed to common metabolism, increases activity and the validity of biochemical system.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of new complex microorganism preparations and the application in wastewater treatment thereof, comprise 38 kinds of different strains, and in practical engineering application through a kind of efficient, the non-secondary pollution biotechnological formulation of screening for many years, domestication.For complex industrial waste water, select the formulated biotechnological formulation of multiple bacterial classification, object is to allow different bacterial classifications assist mutually in biochemical system complicated pollutent is formed to common metabolism, increases activity and the validity of biochemical system.Complex microorganism preparations of the present invention, to trade effluent, especially has good Degradation to the organic pollutant of some difficult degradations (as phenyl ring halogenated compound), to ammonia nitrogen, goes protection ratio all more than 99%.
The present invention adopts following technical scheme for solving its technical problem:
On the one hand, the present invention relates to a kind of complex microorganism preparations, it is characterized in that, each raw materials quality per-cent of described complex microorganism preparations is: benzene bacillus 3.5%, sulfolobus acidocaldarius 2.7%, swelling pimelobacter sp 2.9%, halococci 2.5%, Nitrospira gracilis 2.9%, lower floor acid bacterium 2.3%, have a liking for salt alkalophilic bacillus 1.3%, have a liking for salt basophilic coccus 1.6%, Alkaliphilic bacillus 1.8%, have a liking for salt capsule bacterium 3%, bacillus firmus 2.4%, metal coccus 2.2%, diligent raw gold goal bacterium 2.9%, have a liking for salt bacillus 2.5%, Rhodopseudomonas palustris 2.1%, white-rot fungi 3.2%, the A Zuoruisi Styx bacterium 1.4% that dwells, have a liking for the richly endowed bacterium 2% of salt, bacillus aceticus 4.1%, acetobacter xylinum 2.5%, Flavobacterium 2.1%, dinitrogen cellulomonas cartae 2.6%, rhodopseudomonas acidophilus 3.2%, movable nitrated coccus 3.1%, movable nitrated coccus 2.6%, rhodopseudomonas acidophilus 2.8%, very thin nitrosification vibrios 3.4%, soccer star micrococcus 3.5%, genus bacillus 3.7%, plant lactobacillus 2.3%, the short shape bacillus 1.9% of excrement, Vickers bacterium nitrobacter 1.7%, achromobacter 3.7%, Achromobacter xylosoxidans subsp.denitrificans 2.6%, Micrococcus halobius 2.8%, have a liking for sour two sides bacterium 3.1%, non-bacillus 4.1% and subtilis 1.0%.
On the other hand, the present invention relates to a kind of application of complex microorganism preparations of the present invention, it is characterized in that, described complex microorganism preparations is for the treatment of waste water, and dosage is: in biochemistry pool, drop into the complex microorganism preparations that inchoate aspect volume concentrations is biochemistry pool processing volume 1.5～4 ‰.
Complex microorganism preparations of the present invention, is applicable to the waste water treatment process such as activated sludge process, contact oxidation method, SBR method, biological filter, MBR, A (anaerobism)/A (oxygen of holding concurrently)/O (aerobic), IC (anaerobism)/A (oxygen of holding concurrently)/O (aerobic).
Compared with prior art, beneficial effect of the present invention is:
(1) complex microorganism preparations of the present invention, comprises 38 kinds of different strains, and in practical engineering application through a kind of efficient, the non-secondary pollution biotechnological formulation of screening for many years, domestication.For complex industrial waste water, select the formulated biotechnological formulation of multiple bacterial classification, object is to allow different bacterial classifications assist mutually in biochemical system complicated pollutent is formed to common metabolism, increases activity and the validity of biochemical system.Complex microorganism preparations of the present invention, to trade effluent, especially has good Degradation to the organic pollutant of some difficult degradations (as phenyl ring halogenated compound).
Embodiment
Below in conjunction with embodiment, further explain the present invention, but embodiment does not limit in any form to the present invention.
Embodiment 1
The present embodiment carries out by the following method:
Waste water is Shanxi coking plant coking chemical waste water, waste water after oil removal by air bubbling is processed, directly enter biological treatment system (, the water-quality guideline that enters biological treatment system is as follows:
COD：3500--6000mg/l
NH
4 +-N：300--350mg/l
PH value: 7.8---9.0
The engineering water yield is 1500m
3/ d, A (anaerobism)/A (oxygen of holding concurrently)/O (aerobic) technique, the processing condition of operation are as follows:
The residence time: 88h, anaerobism 20h, the oxygen 20h that holds concurrently, aerobic 48h
Temperature: 25-35 ℃
DO：2～5mg/l
In biochemistry pool, drop into the complex microorganism preparations that initial concentration is biochemistry pool processing volume 3 ‰ (volume ratio).
After disposable adding, after steady running in 30～60 days, the clearance of COD in coking chemical waste water is maintained to 98% left and right, the clearance of ammonia nitrogen is remained on to 99% level, to the clearance of total nitrogen in 88% left and right.
Embodiment 2
The present embodiment carries out by the following method:
Waste water is Jiangyin pharmacy corporation, Wastewater in Biologic Pharmacy, and waste water, after materialization regulates PH to process, directly enters biological treatment system, and the water-quality guideline that enters biological treatment system is as follows:
COD：9000--14000mg/l
NH
4 +-N：900--1600mg/l
Chlorion: 20000mg/l
PH value: 8.0---9.5
The engineering water yield is 600m
3/ d, UASB (anaerobism)/A (oxygen of holding concurrently)/O (aerobic) technique, the processing condition of operation are as follows:
The residence time: 115h, anaerobism 48h, the oxygen 16h that holds concurrently, aerobic 48h
Temperature: 25-35 ℃
Aerobic DO:2～5mg/l
In biochemistry pool, drop into the complex microorganism preparations that initial concentration is biochemistry pool processing volume 4 ‰ (volume ratio).
After disposable adding, after steady running in 10～30 days, the clearance of COD in Wastewater in Biologic Pharmacy is maintained to 97% left and right, the clearance of ammonia nitrogen is remained on to 99.99% level, tolerance chlorine ion concentration reaches 20000mg/l.
Embodiment 3
The present embodiment carries out by the following method:
Waste water is the waste water of Shaanxi Paint Factory, and waste water, after materialization oil removal treatment, directly enters biological treatment system, and the water-quality guideline that enters biological treatment system is as follows:
COD：12000--27000mg/l
Sulfate ion: 5000mg/l
PH value: 7.5---9.5
The engineering water yield is 200m
3/ d, A (anaerobism)/A (oxygen of holding concurrently)/O (aerobic) technique, the processing condition of operation are as follows:
The residence time: 80h, anaerobism 12h, the oxygen 20h that holds concurrently, aerobic 48h
Temperature: 25-35 ℃
DO：2～5mg/l
In biochemistry pool, drop into the complex microorganism preparations that initial concentration is biochemistry pool processing volume 2.5 ‰ (volume ratio).
After disposable adding, after steady running in 30～60 days, the clearance of COD in waste water is maintained to 97% left and right, the clearance of ammonia nitrogen is remained on to 99% level, to the clearance of total nitrogen in 84% left and right.
Embodiment 4
The present embodiment carries out by the following method:
Waste water is Shanxi pharmaceutical factory pharmacy waste water, and waste water, after materialization slagging-off is processed, directly enters biological treatment system, and the water-quality guideline that enters biological treatment system is as follows:
COD：5000--9000mg/l
NH
4 +-N：200--300mg/l
PH value: 8.0---9.5
The engineering water yield is 1500m
3/ d, UASB (anaerobism)/A (oxygen of holding concurrently)/O (aerobic) technique, the processing condition of operation are as follows:
The residence time: 112h, anaerobism 48h, the oxygen 16h that holds concurrently, aerobic 48h
Temperature: 25-35 ℃
Aerobic DO:2～5mg/l
In biochemistry pool, drop into the complex microorganism preparations that initial concentration is biochemistry pool processing volume 1.5 ‰ (volume ratio).
After disposable adding, after steady running in 20～50 days, the clearance of COD in pharmacy waste water is maintained to 99.9% left and right, the clearance of ammonia nitrogen is remained on to 99% level.
Visible; complex microorganism preparations of the present invention has good Degradation to the organic pollutant of some difficult degradations (as phenyl ring halogenated compound); to the clearance of COD in waste water all more than 97%; especially the clearance of COD in pharmacy waste water is reached to 99.9%; to ammonia nitrogen go protection ratio all more than 99%, it has extraordinary effect to wastewater treatment.
Under design of the present invention, there are multiple enforcement and expression-form, or adopt the above-mentioned replacement scheme that is equal to all to fall into protection scope of the present invention.
Claims (2)
1. a complex microorganism preparations, is characterized in that, each raw materials quality per-cent of described complex microorganism preparations is: benzene bacillus 3.5%, sulfolobus acidocaldarius 2.7%, swelling pimelobacter sp 2.9%, halococci 2.5%, Nitrospira gracilis 2.9%, lower floor acid bacterium 2.3%, have a liking for salt alkalophilic bacillus 1.3%, have a liking for salt basophilic coccus 1.6%, Alkaliphilic bacillus 1.8%, have a liking for salt capsule bacterium 3%, bacillus firmus 2.4%, metal coccus 2.2%, diligent raw gold goal bacterium 2.9%, have a liking for salt bacillus 2.5%, Rhodopseudomonas palustris 2.1%, white-rot fungi 3.2%, the A Zuoruisi Styx bacterium 1.4% that dwells, have a liking for the richly endowed bacterium 2% of salt, bacillus aceticus 4.1%, acetobacter xylinum 2.5%, Flavobacterium 2.1%, dinitrogen cellulomonas cartae 2.6%, rhodopseudomonas acidophilus 3.2%, movable nitrated coccus 3.1%, movable nitrated coccus 2.6%, rhodopseudomonas acidophilus 2.8%, very thin nitrosification vibrios 3.4%, soccer star micrococcus 3.5%, genus bacillus 3.7%, plant lactobacillus 2.3%, the short shape bacillus 1.9% of excrement, Vickers bacterium nitrobacter 1.7%, achromobacter 3.7%, Achromobacter xylosoxidans subsp.denitrificans 2.6%, Micrococcus halobius 2.8%, have a liking for sour two sides bacterium 3.1%, non-bacillus 4.1% and subtilis 1.0%.
2. the application of a complex microorganism preparations claimed in claim 1, it is characterized in that, described complex microorganism preparations is for the treatment of waste water, and dosage is: in biochemistry pool, drop into the complex microorganism preparations that inchoate aspect volume concentrations is biochemistry pool processing volume 1.5～4 ‰.
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| CN105417732A (en) * | 2016-01-07 | 2016-03-23 | 上海心喜乐生态科技有限公司 | Method and application for using compound microorganisms for landfill leachate treatment |
| CN105779344A (en) * | 2016-03-29 | 2016-07-20 | 江苏蓝星化工环保股份有限公司 | Composite microbial agent |
| CN106635883A (en) * | 2016-11-09 | 2017-05-10 | 东营晟宇环保科技有限公司 | Composite microbial preparation for treating watercourse organic matter |
| CN106906156A (en) * | 2017-01-10 | 2017-06-30 | 北京师范大学 | Microbial inoculum for processing coal-to-olefin waste water |
| CN107265670A (en) * | 2017-08-21 | 2017-10-20 | 苏州神良生物科技有限公司 | One kind processing waste water composite biological agent |
| CN107418907A (en) * | 2017-04-11 | 2017-12-01 | 辽宁科技大学 | A kind of microbial bacterial agent and its application method of gasoline petroleum-like hydrocarbon of degrading |
| CN107787360A (en) * | 2015-02-27 | 2018-03-09 | 阿坤纳斯公司 | Microorganism consortium |
| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
| CN110510759A (en) * | 2019-09-16 | 2019-11-29 | 德蓝水技术股份有限公司 | A kind of water process hydrolysis acidification composite bacteria agent and preparation method thereof |
| CN110699291A (en) * | 2019-11-04 | 2020-01-17 | 浙江树人学院（浙江树人大学） | Achromobacter xylosoxidans with sulfide degradation performance and application thereof |
| CN111547847A (en) * | 2020-05-06 | 2020-08-18 | 南京师盛生态环境研究院有限公司 | Device and method for removing total nitrogen in sewage by composite microorganism enhanced A/O (anaerobic/aerobic) process |
| CN111661929A (en) * | 2020-06-05 | 2020-09-15 | 北京赛富威环境工程技术有限公司 | Organic waste treatment method |
| CN113336334A (en) * | 2021-05-31 | 2021-09-03 | 辽宁大学 | Bacillus firmus and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene |
| CN114736816A (en) * | 2021-04-30 | 2022-07-12 | 厦门百仕洁环保科技有限公司 | Salt-tolerant composite strain for water treatment and application thereof |
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2014
- 2014-09-12 CN CN201410464776.9A patent/CN104195090A/en active Pending
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| CN107787360A (en) * | 2015-02-27 | 2018-03-09 | 阿坤纳斯公司 | Microorganism consortium |
| US11230506B2 (en) | 2015-02-27 | 2022-01-25 | Amvac Chemical Corporation | Microbial consortia |
| CN105417732A (en) * | 2016-01-07 | 2016-03-23 | 上海心喜乐生态科技有限公司 | Method and application for using compound microorganisms for landfill leachate treatment |
| CN105779344A (en) * | 2016-03-29 | 2016-07-20 | 江苏蓝星化工环保股份有限公司 | Composite microbial agent |
| CN106635883A (en) * | 2016-11-09 | 2017-05-10 | 东营晟宇环保科技有限公司 | Composite microbial preparation for treating watercourse organic matter |
| CN106906156A (en) * | 2017-01-10 | 2017-06-30 | 北京师范大学 | Microbial inoculum for processing coal-to-olefin waste water |
| CN107418907B (en) * | 2017-04-11 | 2020-06-30 | 辽宁科技大学 | Microbial agent for degrading gasoline petroleum hydrocarbon and use method thereof |
| CN107418907A (en) * | 2017-04-11 | 2017-12-01 | 辽宁科技大学 | A kind of microbial bacterial agent and its application method of gasoline petroleum-like hydrocarbon of degrading |
| CN107265670A (en) * | 2017-08-21 | 2017-10-20 | 苏州神良生物科技有限公司 | One kind processing waste water composite biological agent |
| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
| CN110156174B (en) * | 2019-05-30 | 2022-01-25 | 杭州秀川科技有限公司 | Mixed strain fermentation biological pretreatment method for high-concentration high-salt pharmaceutical wastewater |
| CN110510759A (en) * | 2019-09-16 | 2019-11-29 | 德蓝水技术股份有限公司 | A kind of water process hydrolysis acidification composite bacteria agent and preparation method thereof |
| CN110699291A (en) * | 2019-11-04 | 2020-01-17 | 浙江树人学院（浙江树人大学） | Achromobacter xylosoxidans with sulfide degradation performance and application thereof |
| CN111547847A (en) * | 2020-05-06 | 2020-08-18 | 南京师盛生态环境研究院有限公司 | Device and method for removing total nitrogen in sewage by composite microorganism enhanced A/O (anaerobic/aerobic) process |
| CN111661929A (en) * | 2020-06-05 | 2020-09-15 | 北京赛富威环境工程技术有限公司 | Organic waste treatment method |
| CN111661929B (en) * | 2020-06-05 | 2021-08-24 | 北京赛富威环境工程技术有限公司 | Organic waste treatment method |
| CN114736816A (en) * | 2021-04-30 | 2022-07-12 | 厦门百仕洁环保科技有限公司 | Salt-tolerant composite strain for water treatment and application thereof |
| CN113336334A (en) * | 2021-05-31 | 2021-09-03 | 辽宁大学 | Bacillus firmus and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene |
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Application publication date: 20141210 |