CN105062936A - Compound halophilous-microorganism bacterium agent and application thereof - Google Patents
Compound halophilous-microorganism bacterium agent and application thereof Download PDFInfo
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- CN105062936A CN105062936A CN201510589228.3A CN201510589228A CN105062936A CN 105062936 A CN105062936 A CN 105062936A CN 201510589228 A CN201510589228 A CN 201510589228A CN 105062936 A CN105062936 A CN 105062936A
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Abstract
The invention discloses a compound halophilous-microorganism bacterium agent and an application thereof. Halophilous microorganisms are the microorganisms which are not inhibited or poisoned by high-concentration inorganic salts in the high-salt environment, can carry out normal growth and metabolism and comprise salt-resistant microorganisms and halophilic microorganisms. The compound halophilous-microorganism bacterium agent at least comprises two of halophilic bacteria, halophilic archaea, salt-resistant bacteria, salt-resistant archaea, halophilic fungi and salt-resistant fungi, wherein the types and the quantity of the halophilous microorganisms are at least 10, and the halophilous microorganisms at least comprise one type of uncultured microorganisms. The compound halophilous-microorganism bacterium agent disclosed by the invention has the advantages that high-efficiency and stable degradation can be carried out on organic matters under the condition of 1%-20% salt; by using the compound halophilous-microorganism bacterium agent for biochemical treatment of high-salt waste water and microorganism restoration of saline-alkaline soil, the pollution treatment cost can be reduced.
Description
Technical field
The invention belongs to environmental protection microbial technology field, be specifically related to a kind of can to organic pollutant thing compound halophile microbial inoculum carrying out efficient stable degraded and uses thereof under hypersaline environment.
Background technology
High-salt wastewater complicated component, inorganic salt wherein produce the growth of common micro-organisms and suppress or toxic action, seriously limit the application of biochemical technology in high-salt wastewater process field.Comprise the materializing strategy technology of evaporation, membrane sepn, ion-exchange, advanced oxidation, electrolysis etc., to Pollutant Treatment is not thorough or cost is higher.High-salt wastewater biochemical treatment has feasibility in theory, but is mainly in the laboratory study stage at present.The difficult point of high-salt wastewater biochemical processing and key are contaminant degradation microorganisms.Halophilic bacterium can carry out normal growth metabolism under hypersaline environment, thus prepares a kind of main method that halophilic bacterium microbial inoculum becomes high-salt wastewater biochemical treatment.
Number of patent application CN201210078420.2 screening and separating from oil drilling slurry obtains a strain addicted to salt genus bacillus (CGMCCNO.4194), this bacterial strain can reach 70% to high salt oil production waste liquid PetroChina Company Limited. clearance under mild alkaline conditions, reaches more than 90% to the petroleum-type clearance in lower concentration waste liquid.
The basic inorganic salt culture medium containing toluene that number of patent application CN201210232811.5 utilizes NaCl to be 1.5M ~ 2.5M, enrichment culture is being carried out to toluene concentration soil, and carry out continuous domestication cultivation with 30% inoculum size under aseptic technique, finally obtain degrading the Halophilic Bacterium group of toluene, and the flora wherein contained comprises
marincobacter,
prolixibacter,
balneolawith
halobacillusin one or more.
Number of patent application CN201210095595.4 utilizes saliferous to be that halophilic bacterium in the milk medium seashore salt field waste water of 10% ~ 15% screens, and therefrom isolate 3 single strains with CM substratum, then be inoculated in bio-oxidation pond and adopt honeycomb filler to cultivate, obtaining halophilic bacterium microbial inoculum is that phenol in the waste water of 20% ~ 25% is degraded to salinity.Halophilic bacterium in crude salt lake water screens, then cultivates with liquid phenol substratum, is then prepared from through SBR domestication, its phenol that can thoroughly degrade in high-salt wastewater within a short period of time.
Number of patent application CN201110003844.8 with 2 ~ 25% containing phenol substratum, from environment screening obtain four kinds of Facultative Halophiles Providences (CGMCCNO.4327), brevibacterium epidermidis (CGMCCNO.4329), Halomonas (CGMCCNO.4330),
oceanobacillussp.cJ-10(CGMCCNO.4328); composite fungus agent is built with it; and fix with granulated active carbon, can process that salt concn is 2 ~ 25%, the waste water of phenol < 2500mg/L, water outlet COD < 200mg/L, phenol < 0.2mg/L.
Number of patent application CN200910266193.4 builds composite fungus agent by screening the three strain Natrinema altunense sp Halomonas CGMCCNo.3081, pseudomonas CGMCCNo.3082 and the genus bacillus CGMCCNo.3083 that obtain, process salinity is the Melamine Production high-salt wastewater of 13% ~ 20%, TOC clearance 94.86%, TOC of yielding water can be reduced to 30 ~ 40mg/L.
Number of patent application CN200410044953.4 utilizes screening from the active sludge of process toluylic acid factory effluent to obtain a strain Halomonas CGMCCNo.1112, this Facultative Halophiles to be cultivated in the microbial inoculum obtained thalline quantity in liquid bacterial agent and is reached more than 1,000,000,000 by fermentation process, be added to toluylic acid factory effluent biochemical treatment tank, it is stabilized in more than 97% to the removal efficiency of toluylic acid.
Whether can carry out being separated and pure culture in prior art condition according to microorganism, microorganism can be divided into two classes: can pure culture microorganism and can not pure culture microorganism.Microorganism major part in physical environment belongs to can not pure culture microorganism.Show based on molecular biological microbial ecology result of study, the environmental microorganism of more than 99% is difficult to carry out under prior art conditions being separated and pure culture.In halophilic bacterium microbial inoculum in above-mentioned prior art citing, halophilic bacterium kind number mostly is 4 kinds most, and halophilic bacterium is all adopt the halophilic bacteria and halophilic archaea that pure culture technigne is separated and pure culture obtains.
Large quantity research shows, the treatment effect of complex micro organism fungicide to waste water is better than the treatment effect of single microbial inoculum.But existing various complex micro organism fungicide, employing be that the different microorganisms bacterial strain of acquisition microorganism being separated pure culture carries out the composite combined microbiobacterial agent obtained.Be characterized in the kind of microorganism in complex micro organism fungicide, quantity and definite functions, the waste water limited types be thus suitable for.The compound Salt-tolerant microbial agent of above-mentioned citing, its microbe species only has 1-4 kind, can not meet the demand of actual high-salt wastewater process.
In prior art, the preparation of microbial inoculum mainly adopts traditional pure culture technigne, and in its preparation process, the cultivation of microbial inoculum is isolated with external environment.Therefore, preparation-obtained microbial inoculum, to the adaptive faculty of environment, only just can be tested in application process, compares and loses time and energy.The present invention adopts and prepare halophile microbial inoculum under open hypersaline environment.So-called open hypersaline environment is for the sterilizing needed in traditional microbial inoculum preparation process, isolated environment.Under the mixed cultivation process of halophile is in open environment, therefore in the culturing process of halophile, be no matter adopt autogamy high-salt wastewater, still adopt actual high-salt wastewater, only have the halophile group adapting to waste water quality and hypersaline environment could by enrichment culture.This cultural method simplifies culturing step and the culture condition of halophile, can not only improve the scope of application and the treatment effect of microbial inoculum, and shorten the preparation cycle of microbial inoculum, reduce the preparation cost of microbial inoculum.
Halophile microbial inoculum provided by the invention, its microorganism forms the salt tolerant bacterium or halophilic bacteria that comprise in microbial taxonomy meaning, generally also containing halophilic fungi, addicted to salt fungi, halophilic archaea, addicted to salt protozoon with addicted to salt metazoan.Traditional halophilic bacterium microbial inoculum is only containing the class in halophilic bacteria, salt tolerant bacterium, halophilic fungi, halophilic archaea or two classes, and halophile microbial inoculum of the present invention comprises addicted to salt protozoon with addicted to salt metazoan toward contact.
Halophile microbial inoculum provided by the invention, employing mixed culturing method is prepared, with hypersaline environment sample for halophile is originated, under open hypersaline environment, carry out the mixing Dual culture of halophile, gained mixed solution is the liquid bacterial agent of halophile.
Halophile microbial inoculum provided by the invention, its preparation process be in fact halophile carry out mixing Dual culture and mixobiosis long.In hypersaline environment halophile carry out surviving mainly with modes such as symbiosis, Co metabolism, alternate, commensalism greatly, metabolism and growth, organism is also degraded by this synergy of different microorganisms.Halophile comprises bacterium, fungi, ancient bacterium, protozoon, metazoan etc., and its mutual relationship comprises cooperation, competes, conditions each other, and forms food chain one by one, thus the salt-durable microbe Microcosmic ecosystem that formation one is complicated.Therefore, the present invention is this not to be separated the halophile bacterial preparation process that purebred halophile is method, and the growth for halophile different in hypersaline environment sample provides one can interactional hypersaline environment; Again by providing suitable material conditions, making enrichment culture process become various halophile population and rebuilding group and ecosystem processes.
Halophile microbial inoculum of the present invention, is prepared under open environment, without the need to carrying out the process such as sterilizing.The benefit done like this is in microbial inoculum preparation process, provide envrionment conditions open like this, and the halophile population making it possible to adapt to external complex environment can obtain competitive edge and breed.
Halophile microbial inoculum of the present invention, is directly prepared under high salt condition.The benefit done like this provides such hypersaline environment in microbial inoculum preparation process, only has the halophile adapting to hypersaline environment could obtain competitive edge and carry out Growth and reproduction.
Halophile microbial inoculum of the present invention, can directly be prepared under total salt 1 ~ 20% condition, and also namely the salt concn of mixed culture enrichment medium used and Selective agar medium is 10 ~ 200g/L.These inorganic salt can be one or more in muriate, vitriol, carbonate, phosphoric acid salt and nitrate.Halophile generally can survive and grow in very wide salinity range, but the optimum growh metabolism salinity range had is narrower than salt tolerant scope, and the optimum growh metabolism salt concentration range that different halophile has there are differences.Therefore, total salinity should keep relative stability in maintain liquid in microbial inoculum preparation process.
Halophile microbial inoculum provided by the invention, wherein halophile be no less than 10 kinds, and wherein can not cultivate halophile and undertaken confirming or identifying by the method not relying on pure culture, such as:
1. biochemical method: lipoid fatty acid (PLFA) is analyzed;
2. physiology method: Biolog Microdilution plate method;
3. molecular biology method: PCR-DGGE/TGGE, PCR-SSCP, PCR-RAPD, PCR-RFLP, T-RFLP, metagenomics etc.
Halophile microbial inoculum provided by the invention, its microbial inoculum form can be liquid, solid-state or be combined with carrier.
Halophile microbial inoculum provided by the invention is relative to the advantage of existing microbial inoculum product:
(1) halophile microbial inoculum provided by the invention, can adapt to different high-salt wastewater water quality and carry out the degraded of efficient stable to wherein organic pollutant, salt tolerant scope reaches 1% ~ 20%, and anti-salinity shock ability is strong.
(2) halophile microbial inoculum provided by the invention, its preparation cycle is short and preparation cost is low.
(3) halophile microbial inoculum provided by the invention, conveniently can carry out pilot scale culture, both can be cultivated by the mode of fermentation, common biochemical facility also can be utilized to cultivate, and solves traditional microbial inoculum and produces the limitation that must rely on fermentation engineering.
Summary of the invention
Technical problem to be solved of the present invention is for the deficiencies in the prior art, provides a kind of new compound halophile microbial inoculum.This compound halophile microbial inoculum has abundant halophile diversity, and waste water quality is adaptable, and anti-salinity shock ability and all more existing microbial inoculum product of anti-load impact ability improve.
Halophile of the present invention, to refer in hypersaline environment and does not suppress by high density inorganic salt or poison, and can carry out the microorganism of normal growth metabolism, comprises salt tolerant and addicted to the bacterium of salt, fungi, ancient bacterium, protozoon and metazoan.
Halophile microbial inoculum of the present invention, refers to the microbiobacterial agent of microorganism composition based on halophile in microbial inoculum.
Technical problem to be solved by this invention is realized by following products scheme.
The invention provides a kind of compound halophile microbial inoculum, the compound microbial microbial inoculum that this microbial inoculum is made up of multiple-microorganism, in its feature microbial inoculum, halophile consists of:
(1) from halophile type, this microbial inoculum is at least by halophilic bacteria, halophilic archaea, salt tolerant bacterium, the ancient bacterium of salt tolerant, form addicted to more than two classes in salt fungi, halophilic fungi or two classes;
(2) whether can pure culture from microorganism, this microbial inoculum is made up of Bacterial diversity and Anticipated transient without scram, and Anticipated transient without scram kind quantity is at least a kind.
Anticipated transient without scram of the present invention, refers to have auxotrophy, or growth metabolism mode is the microorganism of symbiosis, alternate, Co metabolism, is present in environment, but can't be separated it under prior art conditions and the microorganism of pure culture.In environment, the kind of Anticipated transient without scram and quantity are far away more than Bacterial diversity, utilize Anticipated transient without scram process waste water and high-salt wastewater to have larger application potential.
In the technical scheme of a kind of compound halophile microbial inoculum of the present invention, preferred technical characteristic comprises:
(1) also comprise in the composition of described microbial inoculum salt tolerant protozoon, addicted to salt protozoon, salt tolerant protozoon, addicted to more than two classes in salt metazoan or two classes.
(2) microbial inoculum described in adopts mixing co-culture method to be prepared.
(3) microbial inoculum described in is for the method disclosed in CN201310540099.X is prepared according to number of patent application.
(4) Anticipated transient without scram described in detects by 16SrRNA order-checking, high-throughput DNA sequencing, microchip, metagenomics and other Protocols in Molecular Biology means.
(5) its microbial population of the microbial inoculum described in is at least 10 kinds.
A kind of compound halophile microbial inoculum provided by the invention, can be applicable to high-salt wastewater process, hypersaline environment water remediation and salt affected soil reparation.
Explanation is further illustrated to content of the present invention below:
In environment, the microorganism of more than 99% belongs to Anticipated transient without scram, can not carry out under prior art conditions being separated and pure culture.Microbiobacterial agent and halophilic microorganism microbial inoculum in prior art, bacterium, fungi in its microbial inoculum composition be all through with ancient bacterium be separated, microorganism that the step such as pure culture obtains.Adopt separation and Culture technology to prepare microbiobacterial agent, its bacterial classification limited source, also constrain the application of microbial inoculum at environmental protection industry (epi).Organic pollutant microbiological deterioration in the environment, in the microorganism participated in, quite a few can't be separated the microorganism with pure culture under belonging to prior art condition.Generally speaking, the degradation effect of multiple-microorganism to same pollutent is better than single microorganism.。
The present inventor adopts the mode of mixed culture to carry out enrichment culture and domestication to environmental microorganism, avoids carrying out separation and Culture to environmental microorganism, and microbial inoculum prepared by mixed culture, and its effect is better than single microbial inoculum, and has greatly saved microbial inoculum preparation cycle.From microorganism microscopic examination effect, wherein there is multiple protozoon in microbial inoculum prepared by mixed culture, the protozoons such as such as ciliate, flagellate, amoeba.And utilize the halophile microbial inoculum that mixed culture mode obtains, can be used for processing different high-salt wastewaters, the adaptive faculty of microbial inoculum widely.
Hypersaline environment is ubiquitous a kind of physical environment on the earth, all large than the low-salt environment that the mankind survived and understood many of its distribution area and range.Microbial ecology research also confirms, on the earth, the diversity of halophile is same abundant with common less salt microorganism.The various biochemical reaction types that in conventional environment, microorganism is responsible for, are also all found in hypersaline environment.But due to the singularity of hypersaline environment, namely its high salt feature, makes the physiological metabolism of suitable high salt microorganism have the feature of himself.Prior art is often treated halophilic bacterium as a kind of peculiar microorganism, thus conventional research method or traditional pure culture technigne means, which has limited the research of halophile actual utility and utilizes level.
Utilize halophilic bacterium process high-salt wastewater to be sewage disposal science and technology and the common recognition of engineering staff, but rarely have engineer applied successful story.The technology that its technical reason is to adopt tradition to be separated pure culture obtains halophilic bacterium, and the kind of halophilic bacterium is less simultaneously, cause halophilic bacterium to waste water treatment effect compare and be difficult to keep stable; Its economic cause is that microbial inoculum preparation cycle is long, microbial inoculum preparation cost is higher, microbial inoculum use cost is higher equally.
Embodiment
A kind of compound halophile microbial inoculum provided by the invention is a kind of based on the compound microbial microbial inoculum for preparing of microorganism mixing Coculture techniques, feature environmentally can carry out self-adaptation and form the superior microorganism group under respective hypersaline environment.
Carry out the inventive method below by specific embodiment to be described further:
Embodiment 1, a kind of compound halophile microbial inoculum, in its microbial inoculum, halophile consists of:
(1) from halophile Population Types, this microbial inoculum is at least by halophilic bacteria, halophilic archaea, salt tolerant bacterium, the ancient bacterium of salt tolerant, form addicted to more than two classes in salt fungi, halophilic fungi or two classes;
(2) whether can pure culture from microorganism, this microbial inoculum is made up of Bacterial diversity and Anticipated transient without scram, and Anticipated transient without scram kind quantity is at least a kind;
Described Anticipated transient without scram refers to have auxotrophy, or growth metabolism mode is the microorganism of symbiosis, alternate, Co metabolism, is present in environment, but can't be separated it under prior art conditions and the microorganism of pure culture
(3) in microbial inoculum, halophile kind quantity is at least 10 kinds.
Embodiment 2, a kind of compound halophile microbial inoculum described in embodiment 1: described microbial inoculum adopts mixing co-culture method to be prepared.
Embodiment 3, in a kind of compound halophile microbial inoculum described in embodiment 2: described microbial inoculum is for the method disclosed in CN201310540099.X is prepared according to number of patent application.
Embodiment 4, in a kind of compound halophile microbial inoculum described in embodiment 2: described compound halophile microbial inoculum, directly be prepared under inorganic total salt 1 ~ 20% condition, namely also the inorganic salt concentration of mixed culture enrichment medium used and Selective agar medium is 10 ~ 200g/L, described inorganic salt be selected from muriate, vitriol, carbonate, phosphoric acid salt and nitrate one or more.
Described compound halophile microbial inoculum can be applied to high-salt wastewater process, hypersaline environment water remediation and salt affected soil reparation.
embodiment 5:a kind of compound halophile microbial inoculum:
(1) microbial inoculum is made up of halophilic bacteria and salt tolerant bacterium.Wherein:
Halophilic bacteria 11 kinds:
halomonasalkaliphila1 kind
, Halomonasvenusta1 kind
, Halomonasaidingensis1 kind
, Halomonasshengliensis1 kind
, Halomonasalimentaria1 kind
, Halomonassp.2 kinds
, Marinobacterdaepoensis1 kind
, Marinobacteralkaliphilus1 kind
, Marinobactersp.2 kinds
;
5 kinds, salt tolerant bacterium:
flavobacteriumkamogawaensis2 kinds
, Flavobacteriumsp.2 kinds
, Citricellasp.2 kinds
.
(2) Anticipated transient without scram 4 kinds in microbial inoculum, is respectively
halomonassp.1 kind
, Marinobactersp.1 kind,
flavobacteriumsp.1 kind,
, Citricellasp.1 kind
.
(3) microbial inoculum microbe species quantity is 16 kinds.
embodiment 6:a kind of compound halophile microbial inoculum:
(1) microbial inoculum is made up of halophilic bacteria and salt tolerant bacterium.Wherein:
Halophilic bacteria comprises:
halomonasalkaliphila, Halomonasvenusta, Halobacilluslocisalis, Halobacillustrueperi, Halobacillussp., Halomonasaquamarina, Halomonasaxialensis, Marinobacterdaepoensis, Marinobacteralkaliphilus, Microbacteriumaquimaris, Microbacteriumesteraromaticum, Marinobacteriumhalophilum, Marinbactersediminum, Marinbactersalsuginis, Marinobacterlipolyticus, Marinobacterflavimaris, Marinobactersp.,
Bacterium comprises salt tolerant:
Flavobacteriumkamogawaensis, Flavobacteriumsp., Citricellasp., Bacillusfirmus, Bacillusflexus, Bacilluslitoralis, Bacillusmarisflavi, Bacillussp..
(2) Anticipated transient without scram 10 kinds
in microbial inoculum.
(3) microbial inoculum microbe species sum 25 kinds.
(4) this microbial inoculum is the method disclosed in CN201310540099.X according to number of patent application, with saline-alkali soil, strand bed mud, shine salt pond bittern for halophile flora source, with glucose, methyl alcohol, phenol, acetic acid, yeast leaching powder, peptone for organic carbon source, under saltiness 5%NaCl condition, mixed culture obtains.
embodiment 7:a kind of compound halophile microbial inoculum:
(1) microbial inoculum is made up of halophilic bacteria, salt tolerant bacterium and salt-toler ant yeast.Wherein:
Halophilic bacteria 26 kinds:
halomonasalkaliphila, Halomonasaquamarina, Halomonasaxialensis, Halobacilluslocisalis, Halomonassp.5 kinds
, Halobacillussp.5 kinds
marinobacteralkaliphilus, Microbacteriumaquimaris, Marinobacteriumhalophilum, Marinbactersediminum, Marinbactersalsuginis, Marinobacterlipolyticus, Marinobacterflavimaris, Marinobactersp.5 kinds
.
12 kinds, salt tolerant bacterium:
flavobacteriumsp.2 kinds
, Citricellasp., Bacillusfirmus, Bacillusflexus, Bacilluslitoralis, Bacillusmarisflavi, Bacillussp.5 kinds
.
10 kinds, yeast:
Candidazeylanoides, Candidatropicalis, Candidaparapsilosis, Aureobasidiumpullulans, Candidaintermedia, Debaryomyceshansenii, Rhodotorulamucilaginosa, Saccharomycescerevisiae, Yarrowialipolytica, Pichiaguilliermondii.
(2) Anticipated transient without scram 15 kinds in microbial inoculum, wherein halophilic bacteria 10 kinds, 5 kinds, salt tolerant bacterium
.
(3) microbial inoculum microbe species sum 48 kinds.
embodiment 8:a kind of compound halophile microbial inoculum:
(1) microbial inoculum is made up of halophilic bacteria, salt tolerant bacterium.Wherein:
Halophilic bacteria 37 kinds:
halomonassp.8 kinds
, Halobacillussp.5 kinds
, Marinobactersp.5 kinds,
brevibacteriumsp.3 kinds,
altermonasmarina2 kinds,
flavobacteriumsp.2 kinds,
maricaulissp.3 kind, Marinibacillussp.3
kind, Pseudoalteromonassp.3
kind, Pseudomonasxanthmarina3
kind.
25 kinds, salt tolerant bacterium:
flavobacteriumsp.2 kinds
, Microbacteriumsp.2 kind., Bacillussp.5 kinds,
micrococcussp.2 kinds,
flavobacteriumsp.2 kinds,
pseudomonassp.3 kinds
, Pseudomonasp3 kinds
, Rhodococcussp.2 kinds
, Sphingomonassp.4 kinds
.
(2) Anticipated transient without scram 15 kinds in microbial inoculum, wherein halophilic bacteria 10 kinds, 5 kinds, salt tolerant bacterium
.
(3) microbial inoculum microbe species sum 62 kinds.
(4) this microbial inoculum is under respectively saltiness 3%, 5%, 10% condition, carries out the microbial inoculum that mixed culture obtains carry out the composite compound halophile microbial inoculum obtained with methyl alcohol, phenol, glucose, Dried Corn Steep Liquor Powder, molasses, yeast leaching powder for carbon source.
embodiment 9:a kind of compound halophile microbial inoculum:
(1) microbial inoculum is made up of halophilic bacteria, halophilic archaea.Wherein:
Halophilic bacteria 37 kinds:
halomonassp.8 kinds
, Halobacillussp.5 kinds
, Marinobactersp.5 kinds,
brevibacteriumsp.3 kinds,
altermonasmarina2 kinds,
flavobacteriumsp.2 kinds,
maricaulissp.3 kinds
, Marinibacillussp.3
kind, Pseudoalteromonassp.3
kind, Pseudomonasxanthmarina3
kind.
Halophilic archaea 8 kinds:
halorubrumchaoviator1 kind
, Halorubrumlitoreum1 kind
., Halorubrumlipolyticum1 kind,
halomonasvariabilis1 kind,
halococcussp.2 kinds,
halobacteriumsp.2 kinds
.
(2) Anticipated transient without scram 15 kinds in microbial inoculum, wherein halophilic bacteria 10 kinds, halophilic archaea 2 kinds
.
(3) microbial inoculum microbe species sum 45 kinds.
embodiment 10:a kind of compound halophile microbial inoculum:
(1) microbial inoculum is made up of halophilic bacteria, salt tolerant bacterium and salt tolerant protozoon.Wherein:
Halophilic bacteria 11 kinds:
halomonasalkaliphila1 kind
, Halomonasvenusta1 kind
, Halomonasaidingensis1 kind
, Halomonasshengliensis1 kind
, Halomonasalimentaria1 kind
, Halomonassp.2 kinds
, Marinobacterdaepoensis1 kind
, Marinobacteralkaliphilus1 kind
, Marinobactersp.2 kinds
;
5 kinds, salt tolerant bacterium:
flavobacteriumkamogawaensis2 kinds
, Flavobacteriumsp.2 kinds
, Citricellasp.2 kinds
Salt tolerant protozoon 6 kinds: flagellate 2 kinds, amoeba 2 kinds, ciliate 2 kinds.
(2) Anticipated transient without scram 4 kinds in microbial inoculum, is respectively
halomonassp.1 kind
, Marinobactersp.1 kind,
flavobacteriumsp.1 kind,
, Citricellasp.1 kind
.。
(3) microbial inoculum microbe species quantity is 22 kinds.
Compound halophile microbial inoculum in embodiment 5, is applied to the phenolic wastewater treatment of saltiness 3%NaCl.Compound halophile microbial inoculum in embodiment 6, is applied to saltiness 5%Na
2sO
4phenolic wastewater treatment.Compound halophile microbial inoculum in embodiment 7, be applied to containing 3% ~ 6%NaCl containing phenol chemical wastewater treatment.Compound halophile microbial inoculum in embodiment 8, is applied to the chemical wastewater treatment containing 8% ~ 12%NaCl.Compound halophile microbial inoculum in embodiment 9, is applied to the chemical wastewater treatment containing 15% ~ 22%NaCl.Compound halophile microbial inoculum in embodiment 10, is applied to the process of saltiness 3% ~ 10% pickling waste water.
Claims (5)
1. a compound halophile microbial inoculum, is characterized in that: in its microbial inoculum, halophile consists of:
(1) from halophile Population Types, this microbial inoculum is at least by halophilic bacteria, halophilic archaea, salt tolerant bacterium, the ancient bacterium of salt tolerant, form addicted to more than two classes in salt fungi, halophilic fungi or two classes;
(2) whether can pure culture from microorganism, this microbial inoculum is made up of Bacterial diversity and Anticipated transient without scram, and Anticipated transient without scram kind quantity is at least a kind;
Described Anticipated transient without scram refers to have auxotrophy, or growth metabolism mode is the microorganism of symbiosis, alternate, Co metabolism, is present in environment, but can't be separated it under prior art conditions and the microorganism of pure culture
(3) in microbial inoculum, halophile kind quantity is at least 10 kinds.
2. a kind of compound halophile microbial inoculum according to claim 1, is characterized in that: described microbial inoculum adopts mixing co-culture method to be prepared.
3. a kind of compound halophile microbial inoculum according to claim 2, is characterized in that: described microbial inoculum is for the method disclosed in CN201310540099.X is prepared according to number of patent application.
4. a kind of compound halophile microbial inoculum according to claim 2, it is characterized in that: described compound halophile microbial inoculum, directly be prepared under inorganic total salt 1 ~ 20% condition, namely also the inorganic salt concentration of mixed culture enrichment medium used and Selective agar medium is 10 ~ 200g/L, described inorganic salt be selected from muriate, vitriol, carbonate, phosphoric acid salt and nitrate one or more.
5. the application of a kind of compound halophile microbial inoculum described in claim 1 or 2 or 3 or 4, is characterized in that: be applied to high-salt wastewater process, hypersaline environment water remediation and salt affected soil reparation.
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| CN105732221A (en) * | 2016-01-11 | 2016-07-06 | 黄山学院 | Microorganism organic fertilizer with saline-alkali soil improving effect and preparation method thereof |
| CN106008821A (en) * | 2016-05-31 | 2016-10-12 | 山东胜伟园林科技有限公司 | Method for carrying out raw soil greening on slight saline and alkaline land by using halophilic microorganism-containing water retaining agent |
| CN106966836A (en) * | 2017-05-09 | 2017-07-21 | 广东丰康生物科技有限公司 | A kind of feature water-soluble fertilizer improved for salt affected soil and its application |
| CN111018133A (en) * | 2019-12-20 | 2020-04-17 | 深圳清谷环境科技有限公司 | Environment-friendly microbial sewage treatment agent and preparation method and application thereof |
| CN111377544A (en) * | 2018-12-29 | 2020-07-07 | 中蓝连海设计研究院有限公司 | High-salinity wastewater treatment process utilizing halophilic bacteria YL5-2 |
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| CN105154350A (en) * | 2015-07-06 | 2015-12-16 | 农业部环境保护科研监测所 | Halotolerant denitrification compound inoculant and preparation method and application thereof |
| CN105154350B (en) * | 2015-07-06 | 2018-01-09 | 农业部环境保护科研监测所 | A kind of salt tolerant denitrification compound bacteria agent and its preparation method and application |
| CN105732221A (en) * | 2016-01-11 | 2016-07-06 | 黄山学院 | Microorganism organic fertilizer with saline-alkali soil improving effect and preparation method thereof |
| CN106008821A (en) * | 2016-05-31 | 2016-10-12 | 山东胜伟园林科技有限公司 | Method for carrying out raw soil greening on slight saline and alkaline land by using halophilic microorganism-containing water retaining agent |
| CN106966836A (en) * | 2017-05-09 | 2017-07-21 | 广东丰康生物科技有限公司 | A kind of feature water-soluble fertilizer improved for salt affected soil and its application |
| CN111377544A (en) * | 2018-12-29 | 2020-07-07 | 中蓝连海设计研究院有限公司 | High-salinity wastewater treatment process utilizing halophilic bacteria YL5-2 |
| CN111377544B (en) * | 2018-12-29 | 2021-12-31 | 中蓝连海设计研究院有限公司 | High-salinity wastewater treatment process utilizing halophilic bacteria YL5-2 |
| CN111018133A (en) * | 2019-12-20 | 2020-04-17 | 深圳清谷环境科技有限公司 | Environment-friendly microbial sewage treatment agent and preparation method and application thereof |
| CN111018133B (en) * | 2019-12-20 | 2022-03-29 | 深圳清谷环境科技有限公司 | Environment-friendly microbial sewage treatment agent and preparation method and application thereof |
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