CN111018133B - Environment-friendly microbial sewage treatment agent and preparation method and application thereof - Google Patents
Environment-friendly microbial sewage treatment agent and preparation method and application thereof Download PDFInfo
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- CN111018133B CN111018133B CN201911326190.5A CN201911326190A CN111018133B CN 111018133 B CN111018133 B CN 111018133B CN 201911326190 A CN201911326190 A CN 201911326190A CN 111018133 B CN111018133 B CN 111018133B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses an environment-friendly microbial sewage treatment agent and a preparation method and application thereof, wherein active components in the treatment agent comprise bacillus subtilis, enterococcus faecalis and yeast, and the ratio of effective viable count of the bacillus subtilis, the enterococcus faecalis and the yeast is 10: 1: 2. The environment-friendly microbial sewage treatment agent utilizes the symbiotic relationship of various microorganisms, and the three microorganisms, namely bacillus subtilis, enterococcus faecalis and saccharomycetes, are mutually matched to carry out sewage purification together. The method not only avoids the unicity of treating pollutants by microorganisms and degrades more kinds of pollutants, but also can be applied to treating different types of sewage, such as domestic sewage, high-concentration organic wastewater, monosodium glutamate factory wastewater, pickle waste salt water and the like, and has wide application range.
Description
Technical Field
The invention relates to the technical field of sewage treatment agents, in particular to an environment-friendly microbial sewage treatment agent and a preparation method and application thereof.
Background
At present, along with the improvement of people's life, more and more mechanized products are produced, the pollution to the environment is more and more serious, and the problem of treating domestic sewage is more and more emphasized. The sewage treatment method is divided into physical treatment, chemical treatment and biological treatment. Biological treatment is increasingly widely used due to the advantages of safe use and no environmental pollution. Although the types of biological treatment methods are not limited at present, such as an activated sludge method, a biofilm method, an anaerobic biological treatment method, and the like in aerobic biological treatment.
However, although the sewage treatment microbial inoculum in the current market is various, including ammonia nitrogen remover, COD degradation agent, denitrifying microbial inoculum, etc., the microbial inoculum has a single structure, most of them only contain 1 or 2 different kinds of microbes, most of these microbes are some common microbial populations, which can have a good effect on the easily-treated sewage in some specific environments, and have a poor effect on the treatment of some sewage containing a large amount of pollutants which are difficult to degrade, such as aromatic hydrocarbons, antibiotics, etc., and in extreme environments (such as high-salinity sewage).
Aiming at the problems, the strain with stronger functions is developed at present to be solved, the microbial population in the water treatment system is analyzed by adopting the modern microbial molecular ecological technology while the specific degradation strain is separated, and the population structure of the microbes in the water treatment system is established, so that the basis and the support are provided for separating and purifying the strain or the flora which has strong specific degradation capability and can coexist with the microbial population in the existing water treatment system.
However, at present, a microbial sewage treatment agent with more diversified strain compositions and higher efficiency is still lacking, and needs to be further improved.
Disclosure of Invention
Aiming at the technical problems, the invention provides an environment-friendly microbial water treatment agent and a preparation method thereof, aiming at solving the problem that the high-efficiency microbial water treatment agent aiming at municipal domestic waste and domestic wastewater is lacked at present.
The invention provides the following technical scheme:
the invention provides an environment-friendly microbial sewage treatment agent, which comprises active ingredients of bacillus subtilis, enterococcus faecalis and yeast, wherein the ratio of effective viable count of the bacillus subtilis, the enterococcus faecalis and the yeast is 10: 1: 2.
The three strains are matched and synergized in the sewage, and the excellent sewage purification effect principle is as follows: the bacillus subtilis has rich enzyme systems including protease, lipase and the like, and can degrade and remove pollutants such as organic acid, nitrogenous organic matters and the like under aerobic conditions; enterococcus faecalis mainly degrades various organic matters and absorbs ammonia nitrogen under an anoxic condition; yeast can absorb heavy metals and nitrogen, and can play a role in aerobic or anoxic conditions. The yeast can be cooperatively matched with the bacillus subtilis under aerobic conditions to jointly degrade organic acid, nitrogenous inorganic matters and nitrogenous organic matters and absorb heavy metals; under the ineffective condition, the yeast and the enterococcus faecalis cooperate and cooperate to degrade various organic matters and absorb ammonia nitrogen and heavy metals. Therefore, the environment-friendly microbial sewage treatment agent with the three bacterial strains can play a role under various environmental conditions to treat various water bodies.
Preferably, the environment-friendly microbial sewage treatment agent further comprises a culture medium mixed with the active ingredient.
Preferably, in the environment-friendly microbial sewage treatment agent, the total effective viable count of the active ingredients is 1 × 109CFU/ml。
In the environment-friendly microbial sewage treatment agent, the culture medium comprises a water treatment catalyst with the mass content of 10-100 ppm, the water treatment catalyst comprises a chelating agent and aluminum nitrate and ferric chloride in a molar ratio of 1: 1-2, and the chelating agent is any 2-3 of 5-50ppm of stearin oxide, 5-50ppm of diethyltriaminepentaacetic acid (DTPA), 5-50ppm of gluconic acid, 5-50ppm of polyacrylic acid (PAA) and 5-50ppm of sodium hydroxamate.
Preferably, the optimal formula of the water treatment catalyst is as follows: flocculating agent: 5-50ppm of aluminum nitrate and 5-50ppm of ferric chloride; chelating agent: 5-50ppm of stearin, 5-50ppm of diethyl triaminepentaacetic acid (DTPA) and 5-50ppm of sodium hydroxamate.
The invention provides an application of the environment-friendly microbial sewage treatment agent in high-salinity sewage treatment, and the application method comprises the following steps: and directly adding the environment-friendly microbial sewage treatment agent into an aerobic pool for treating high-salinity sewage, or simultaneously adding nutrient substances to reduce ammonia nitrogen or total nitrogen.
Preferably, the nutrient substances comprise the following components in percentage by mass of the microbial sewage treatment agent: 0.01-0.2% of urea, 0.05-0.5% of phosphate and 0.02-0.2% of yeast extract.
The invention also provides a preparation method of the environment-friendly microbial water treatment agent, which comprises the following steps:
s1: respectively culturing the bacillus subtilis, the enterococcus faecalis and the yeast to a plateau stage;
s2: preparing the culture medium, and sequentially adding 10 parts of beef extract, 30 parts of molasses, 10 parts of glucose, 10 parts of peptone and 2 parts of KH (potassium hydrogen fluoride) into 1000 parts of water2PO4And 4 parts of NaCl, adjusting the pH value to 6.2-6.8, sterilizing at 120 ℃ for 20min, cooling to 37 ℃, and adding a water treatment catalyst;
s3: uniformly mixing the bacillus subtilis, the enterococcus faecalis and the yeast which are cultured to a plateau stage according to a predetermined proportion, then adding the culture medium with the temperature of 37 ℃, uniformly mixing, and culturing for 72 hours under the condition of stirring.
The invention has the following beneficial effects:
1. the invention adopts the symbiotic technology of various microorganisms, so that three microorganisms, namely bacillus subtilis, enterococcus faecalis and saccharomycetes, can be symbiotically generated in one environment, are not mutually antagonistic and can be rapidly propagated. Therefore, the unicity of treating pollutants by microorganisms is avoided, more kinds of pollutants are degraded, and the method can be applied to treating different types of sewage, such as domestic sewage, high-concentration organic wastewater, monosodium glutamate factory wastewater, pickle waste salt water and the like, and has wide application range.
2. Three microorganisms in the treating agent can grow and propagate under the condition of the temperature of less than 10 ℃, the lethal temperature is higher than 35 ℃, the temperature application range is wide, and the microbial water treating agent can be used for treating domestic sewage in cold regions.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The experimental procedures used in the following examples are all conventional procedures unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Experimental materials:
the Bacillus subtilis and enterococcus faecalis in the active ingredients are purchased from Beijing Baiohobowei biotechnology limited; yeasts are available from Shaanxi Fuji Biotech Ltd.
The experimental method comprises the following steps:
1. the detection method of reducing sugar is based on GB/T5009.7-1985;
2. the COD detection method is based on GB 11914-89;
3. the test method of BOD of the water body comprises the following steps: ISO5815-1983, determination of biochemical oxygen demand (BOD5) of water quality five days.
Example 1
(1) The preparation method of the microbial water treatment agent provided by the embodiment comprises the following steps:
s1: respectively culturing the bacillus subtilis, the enterococcus faecalis and the yeast to a plateau stage;
s2: preparing the culture medium, and sequentially adding 10 parts of beef extract, 30 parts of molasses, 10 parts of glucose, 10 parts of peptone and 2 parts of KH (potassium hydrogen fluoride) into 1000 parts of water2PO4And 4 parts of NaCl, adjusting the pH value to 6.2-6.8, sterilizing at 120 ℃ for 20min, cooling to 37 ℃, and adding a water treatment catalyst;
s3: uniformly mixing the bacillus subtilis, the enterococcus faecalis and the yeast which are cultured to a plateau stage according to a predetermined proportion, then adding the culture medium at 37 ℃, uniformly mixing, and culturing for 72 hours under a stirring condition to finally obtain the microbial water treatment agent.
Wherein, the formula of the water treatment catalyst is as follows: the chelating agent is 10ppm of aluminum nitrate and 15ppm of ferric chloride, wherein the chelating agent is 50ppm of oxidized stearin, 20ppm of diethyl triaminepentaacetic acid (DTPA) and 30ppm of sodium hydroxamate.
(2) The sewage treatment test method comprises the following steps:
taking the same amount of sewage of a certain sewage treatment plant, setting the ratio of effective viable count of three strains in each treatment group according to the following table 1, wherein the total nitrogen amount of the sewage in each treatment group before treatment is 146mg/L, the ammonia nitrogen amount is 61mg/L, each treatment group is respectively added with the microbial treatment agent of the invention, the total bacterial amount is ensured to be 200 multiplied by 109CFU/g, and the experimental result is shown in the following table 1:
TABLE 1 effect of sewage treatment
From the above results, it is understood that when the ratio of the effective viable count of Bacillus subtilis, enterococcus faecalis and yeast is 10: 1: 2, the degradation amount of total nitrogen and ammonia nitrogen in sewage is the largest, and the sewage treatment effect of the microbial water treatment agent is the best.
Example 2
The microbial water treatment agent in the embodiment is prepared according to the preparation method of the embodiment 1, the 9 th, 10 th and 11 th treatment groups are arranged, the effective viable count ratio of bacillus subtilis, enterococcus faecalis and saccharomycetes in all the treatment groups is 10: 1: 2, the three treatment groups are different in catalyst, the composition of the catalyst in each group is shown in the following table 2, the total nitrogen content of sewage in each treatment group before treatment is 146mg/L, the ammonia nitrogen content is 61mg/L, the three treatment groups are respectively subjected to a sewage treatment effect test according to the sewage treatment test method of the embodiment 1, and the test results of the treatment groups A, B and C are shown in the table 2:
TABLE 2 formula of water treatment catalyst and sewage treatment effect
From the results of the treatments in Table 2 above, it can be seen that: in the three treatment groups, the catalyst in the treatment group 11 has the best effect, the microbial water treatment agent has the best sewage treatment effect, and the reduction range of the total nitrogen and ammonia nitrogen content is the largest after treatment, which indicates that the optimal formula of the catalyst in the treatment combination is as follows: 40ppm of aluminum nitrate, 40ppm of ferric chloride, 50ppm of stearin oxide, 20ppm of DTPA and 30ppm of sodium hydroxamate.
Example 3
The treatment objects of the embodiment are as follows: the main components of the high-concentration organic wastewater generated in the production process of producing the xylitol by hydrolyzing and hydrogenating the corncobs are nontoxic and harmless reducing sugar and organic acid obtained by hydrolyzing plant fibers. If the corncob is pretreated by adopting an anaerobic sludge bed, the foaming phenomenon is very serious, and the effect is not ideal.
The temperature of the organic wastewater is 30-36 ℃, the pH value is 4-8.5, and due to the fact that the COD concentration is high and other nutrients are insufficient, in the process of treating the organic wastewater by the microbial water treatment agent, a certain weight volume ratio of nutrients (0.1% of urea, 0.2 thousandth of phosphate and 0.1% of yeast extract are added, the concentration is the mass percentage of each component in the microbial water treatment agent), and after flocculation precipitation of the wastewater, the microbial treatment agent (0.1 thousandth of mass volume ratio) is added for aeration for 36 hours. Experiments show that BOD is reduced by 90%.
Example 4
The test object of this example was wastewater from monosodium glutamate factory. Specifically, the microbial treatment agent prepared in the treatment group 5 of example 1 was used for wastewater treatment. After the microorganism treating agent is used for treating for 24 hours, the COD and the reducing sugar content of the wastewater are measured. The amount of the microbial treatment agent added to the wastewater tank was 0.1% o by volume (weight/volume ratio) of the tank volume.
The experimental results are as follows: when the pH value of the wastewater is 4-9 and the temperature is 18-22 ℃, the COD value is reduced by 56 percent, and the reducing sugar removal rate reaches 64 percent; when the pH is 4.0-5.0 and the temperature is 28-36 ℃, the reducing sugar is reduced from 2510.7mg/L to 105.3mg/L, the COD is reduced from 16136mg/L to 2461mg/L, the removal rate of the COD reaches 85%, the removal rate of the reducing sugar reaches 95%, and the treatment effect is far higher than that of an activated sludge method.
Example 5
In this example, 0.1% per mill of the microbial inoculum was inoculated into activated sludge from a municipal sewage treatment plant for sewage treatment, and specifically, the microbial treatment agent prepared in treatment group 5 of example 1 was used for wastewater treatment. Under the condition of 4-5 ℃, after the microorganism water treatment agent is used for treating for 72 hours, the removal rate of COD reaches 75-82%.
Example 6
In this example, the microbial water treatment agent of the present invention was used to treat waste brine in the production of kimchi, specifically, the microbial water treatment agent prepared in the treatment group 5 of example 1 was used. Under the condition that the NaCl concentration is 10 percent (w/v) and the air temperature is 28-33 ℃, the adding amount of the microbial inoculum is 0.1 per mill, aeration is carried out, and after 24 hours of treatment, the BOD removal rate of the waste brine reaches 91 percent.
It should be understood that the technical solutions and concepts of the present invention may be equally replaced or changed by those skilled in the art, and all such changes or substitutions should fall within the protection scope of the appended claims.
Claims (7)
1. An environment-friendly microbial sewage treatment agent comprises active ingredients and a culture medium, and is characterized in that: the active ingredients comprise bacillus subtilis, enterococcus faecalis and yeast, and the ratio of the effective viable count of the bacillus subtilis, the enterococcus faecalis and the yeast is 10: 1: 2; the culture medium comprises a water treatment catalyst with the mass content of 10-100 ppm, the water treatment catalyst comprises a chelating agent, and aluminum nitrate and ferric chloride in a molar ratio of 1: 1-2, wherein the chelating agent is 2-3 of 5-50ppm of stearine oxide, 5-50ppm of diethyl triaminepentaacetic acid (DTPA), 5-50ppm of gluconic acid, 5-50ppm of polyacrylic acid (PAA) and 5-50ppm of sodium hydroxamate.
2. The environment-friendly microbial sewage treatment agent according to claim 1, wherein the total effective viable count of the active ingredients is 1 x 109 CFU/ml。
3. The environment-friendly microbial wastewater treatment agent according to claim 1, wherein the catalyst comprises the following components: 5-50ppm of aluminum nitrate, 5-50ppm of ferric chloride, 5-50ppm of stearin oxide, 5-50ppm of diethyl triaminepentaacetic acid (DTPA) and 5-50ppm of sodium hydroxamate.
4. The use of the environmentally friendly microbial wastewater treatment agent of any one of claims 1 to 3 in high salinity wastewater treatment, wherein the use method comprises: and directly adding the environment-friendly microbial sewage treatment agent into an aerobic pool for high-salinity sewage treatment, or simultaneously adding nutrient substances.
5. The application of the environment-friendly microbial sewage treatment agent in high-salinity sewage treatment is characterized in that the nutrient substances comprise the following components in percentage by mass of the microbial sewage treatment agent: 0.01-0.2% of urea, 0.05-0.5% of phosphate and 0.02-0.2% of yeast extract.
6. The method for preparing an environment-friendly microbial water treatment agent according to any one of claims 1 to 2, comprising the steps of:
s1: respectively culturing the bacillus subtilis, the enterococcus faecalis and the yeast to a plateau stage;
s2: preparing the culture medium, and sequentially adding 10 parts of beef extract, 30 parts of molasses, 10 parts of glucose, 10 parts of peptone and 2 parts of KH (potassium hydrogen fluoride) into 1000 parts of water2PO4And 4 parts of NaCl, adjusting the pH value to 6.2-6.8, sterilizing at 120 ℃ for 20min, cooling to 37 ℃, and adding a water treatment catalyst;
s3: uniformly mixing the bacillus subtilis, the enterococcus faecalis and the yeast which are cultured to a plateau stage according to a predetermined proportion, then adding the culture medium with the temperature of 37 ℃, uniformly mixing, and culturing for 72 hours under the condition of stirring.
7. Use of the environmentally friendly microbial wastewater treatment agent of any one of claims 1 to 3 in wastewater treatment.
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