KR20090062759A - Microbial agents for processing or refractury purifying wastewater using food waste leachate and its manufacturing method - Google Patents

Abstract

A composition for treating or purifying refractory wastewater allows food waste leachate to be used environmentally friendly and can be used usefully in the improvement of a function of treating or purifying refractory wastewater. A method of preparing a leachate from food waste comprises the steps of: contacting two or more bacteria selected from the group consisting of Enterococcus faecium, Enterococcus faecalis, Saccharomyces cerevisiae, Candida utilis and Bacillus subtilis, or cultures thereof with food waste to cultivate the food waste; and crushing and centrifugal separating the cultivated culture fluid and separating a supernatant from the culture fluid to obtain a leachate.

Description

TECHNICAL APPLICATIONS FOR MANUFACTURING DISTRIBUTION WASTEWARE OR WASTEWATER CURRENCY USING FOOD WASTE TREATMENT AND MANUFACTURING METHOD THEREOF {MICROBIAL AGENTS FOR PROCESSING OR REFRACTURY PURIFYING

The present invention provides a method for preparing a desorption solution from food waste, a method for preparing a bad smell reducing seed solution from the prepared desorption solution, a method for preparing a hardly degradable wastewater treatment or waste water purification composition from the prepared seed solution, and the prepared composition. The present invention relates to a method for use in hardly degradable wastewater treatment or wastewater purification.

In 2004, the amount of food waste generated in Korea is about 4.1 million tons in 2004, and the amount of food waste generated per day is about 11,000 tons, which is equivalent to 1400 to 8 tons of trucks. Looking at the contents of food waste, it consists of 53% vegetables, 29% fish, 13% cereals and 5% fruits ( http://lifewaste.me.go.kr . 2000, Ministry of Environment). Since the waste consists of about 80% water and 20% organic matter, the BOD of food desorption liquid generated during the treatment process is very high, ranging from 60,000 ppm to 100,000 ppm, which is an important cause of water pollution. It is becoming.

The treatment of food waste by type can be divided into feed, compost, solid fuel, landfill, incineration and extinction.

In the case of feed, there is a method of replacing food waste itself containing moisture or drying part thereof to replace a part of the existing compound feed, but when it contains water, it is not effective due to storage and handling transportability problems, and In the case of high salt and a large amount of spices can cause gastrointestinal disorders, diarrhea, etc. of the livestock is difficult to secure stability as a feed.

In the case of composting, it is a method of recycling organic waste resources or discharging compost fermented every day or every few days, which causes inconvenience to consumers due to frequent discharge, and increases the amount of compost produced, so that real users are near. In urban centers where traffic is not abundant and the traffic is complicated, the disposal is also inevitable.

In the case of solidifying fuel (Refuse Derived Fuel, RDF), it is not economical because it is recycled as a solid fuel by using a large amount of heat energy contained in food, or because the removal cost and compression molding cost of a large amount of water in food are high. It is also difficult to be practical due to low efficiency.

In the case of landfills, food wastes generated from more than a unit of time since 2005 are not only prohibited from landfilling (modified waste management law in 2005), but also contaminate soil and water quality due to leachate during landfilling.

In the case of incineration, food waste has a very high water content of 80%, which consumes a lot of energy costs when incinerated, and there are problems such as air pollution problems such as dioxins and burden on facility costs.

In the case of annihilation, food is fully fermented by adding food for a long period (1 to 6 months) under perfect aerobic conditions and proper processes. However, there is a disadvantage that the fermentation process takes a long time.

Therefore, the technical problem to be achieved by the present invention is to provide a method for producing a desorption liquid that can be used environmentally friendly desorption liquid when processing food waste.

Another technical problem of the present invention is to provide a method for producing a malodor reducing seed solution from the desorption solution.

Another technical problem of the present invention is to provide a odor reducing seed solution prepared using the desorption solution.

Another technical problem of the present invention is to provide a method for producing a composition for hardly degradable wastewater treatment or wastewater purification from the seed liquid.

Another technical problem of the present invention is to provide a composition for hardly degradable wastewater treatment or wastewater purification prepared using the seed solution.

Another technical problem of the present invention is to provide a method for treating hardly degradable wastewater or treating wastewater using the composition.

In order to achieve the above object, the present invention provides a method for producing a detachment liquid from food waste. The desorption solution may be cultured by inoculating microorganisms capable of hydrolyzing high concentrations of organic and suspended solids contained in food waste.

In addition, the present invention provides a method for producing a malodor reducing seed solution from the detachment solution. The spawn solution may be cultured by inoculating microorganisms that can effectively remove strong odor generated by fermentation of food waste and / or microorganisms.

In addition, the present invention provides a odor reducing seed solution prepared from the desorption liquid. The odor reducing seed solution is characterized in that it has a odor intensity of the degree of detection (Threshold) (see Table 2).

In addition, the present invention provides a method for producing a composition for hardly degradable wastewater treatment or wastewater purification from the seed solution. The composition may be cultured by inoculating a microorganism capable of effectively removing the hardly degradable material present in the hardly degradable wastewater.

In addition, the present invention provides a composition for treating hardly degradable wastewater or wastewater prepared from the seed solution. The composition may be prepared by mixing the spawn solution, the hardly degradable bacteria or cultures thereof, and the hardly degradable wastewater in an appropriate ratio.

The present invention also provides a hardly degradable wastewater treatment or wastewater purification method using the composition.

Hereinafter, the present invention will be described in detail.

"Food waste" is a general term for food waste or food waste that is produced by refining food at home or in a restaurant, leftovers from food, food discarded during distribution, and the lapse of the distribution period, and refers to solids and solids. Excluding liquids (also called "desorption liquids"). The "desorption liquid" refers to leachate from food waste treatment, and means liquid wastewater that cannot be used in daily life or industrially in that state.

Up to now, food waste desorption liquid has been discharged after purification at land wastewater treatment plant.However, due to the difficulty of treatment due to bad odor and the high cost of treatment, it has been designated as one of the east coast and two west coasts. It is becoming. However, as the amount of marine dumping increases rapidly, food waste degreasing solutions become a major cause of marine pollution. Moreover, the food waste desorption liquid is rarely recycled.In particular, according to the Marine Pollution Prevention Act, which is effective from July 1, 2007, the ocean dumping standard of the desorption liquid generated from recycling food waste is 90% to 95% of water content. As it is strengthened to more than%, the cost of marine dumping of the desorption liquid is rapidly increased, and many difficulties are expected in the treatment of the desorption liquid.

Accordingly, the present inventors inoculated and cultured microorganisms capable of efficiently decomposing food waste, in order to effectively treat the food waste desorption solution that has a lot of difficulty in treating, as well as to effectively use the desorption solution for hardly degradable wastewater treatment or waste water purification. To prepare a desorption solution, and sequentially inoculate and incubate microorganisms capable of removing odors in the prepared desorption solution to prepare a odor reducing seed solution, and inoculate and incubate microorganisms useful for hardly degradable wastewater treatment in the prepared seed liquid. The present invention has been completed by preparing compositions useful for degradable wastewater treatment or wastewater purification.

That is, the present invention, first, has an object to efficiently extract or prepare the desorption liquid from food waste. When the extraction rate of the desorption liquid from the food waste is increased, the decomposition of organic matter, cellulose and fats in the food waste is promoted, so that the food waste can be efficiently decomposed in a very short period of time.

To this end, in the present invention

1) Two or more bacteria selected from the group consisting of food waste:

That is, Enterococcus faecium ), Enterococcus faecalis ), Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) contacting two or more bacteria selected from the group consisting of or a culture thereof; And

2) provides a method for preparing a detachment solution from food waste, by crushing and centrifuging the cultured culture to obtain a supernatant to obtain a detachment solution.

In the method of preparing the desorption liquid, the bacteria used in step 1) are hydrolyzed at high speed with high concentrations of organic and inorganic substances contained in food waste, thereby reducing the volume of solids, and reducing the generated carbon gas and / or nitrogen gas. Microorganisms, such as the entero, which can effectively remove and provide conditions suitable for the proliferation of lactic acid bacteria during the subsequent process step, that is, the process step of preparing malodor reducing seed solution from the leachate by synthesizing or secreting sugars, amino acids and vitamins. Caucasus Passium ( Enterococcus) faecium ), Enterococcus faecalis), saccharose in my process serenity non - (Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) can be used two or more species or a culture thereof selected from the group consisting of,

More preferably, Saccharomyces ( Saccharomyces) having an initial resolution of contact with food waste among the bacteria described above cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Enterococcus ) Entericococcus is one or more bacteria or their cultures selected from the group consisting of, and enterococcus that can inhibit the growth of decay bacteria by inducing a decrease in pH faecium ) and Enterococcus faecalis may be used by mixing one or more lactic acid bacteria or their cultures selected from the group consisting of, even more preferably all of the five bacteria described above or a culture thereof. Can be used.

In the culture used in the present invention, the production of desorption liquid from food waste, the production of odor reducing seed liquid from the desorption liquid, or the production of a hardly degradable wastewater treatment or waste water purification composition from the seed liquid, proliferation of bacteria to be inoculated And in order to effectively induce the function, the single strain culture medium or the mixed strain culture solution in which each single strain culture solution is cultured in the culture medium for the normal culture is collectively.

In step 1), the method of contacting the bacteria or cultures thereof with food waste is not particularly limited, and may be carried out by spray inoculation so that the bacteria or cultures thereof can be sufficiently in contact with the food wastes. Preferably, food waste and two or more bacteria or cultures thereof may be contacted at 90:10 to 99: 1 wt%, and more preferably, food waste and two or more bacteria or their cultures are 95: 5 to 98. It can be contacted by 2 weight%.

Second, the present invention has an object to prepare a malodor reducing spawn liquid from the prepared releasing solution.

To this end, in the present invention

1) preparing a stripping solution from food waste;

2) culturing the sequencing solution and lactic acid bacteria (primary bacteria) or culture thereof, and photosynthetic bacteria (secondary bacteria) or culture thereof in a sequential contact with a volume ratio of 90:10 to 99: 1; And

3) It provides a method for producing a odor reducing seed solution from food waste desorption solution to precipitate the cultured culture to obtain a supernatant to obtain a supernatant.

In the spawn solution production method, the bacteria inoculated into food waste during preparation of the desorption liquid of step 1) are not particularly limited, and the high concentration of organic and inorganic substances included in the food waste is rapidly hydrolyzed to solids. Reduces the volume, effectively removes the generated carbon and / or nitrogen gas, synthesizes or secretes sugars, amino acids and vitamins, and inoculates the lactic acid bacterium in the subsequent process step, that is, the lysate, to induce conditions suitable for the growth of lactic acid bacteria. Microorganisms that can provide, preferably Enterococcus faecium ), Enterococcus faecalis , Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) may be used at least one or a culture thereof selected from the group consisting of,

More preferably, Saccharomyces ( Saccharomyces) having an initial decomposition capacity in contact with food waste of the above-described bacteria cerevisiae), Candida utility's (Candida utilis ) and Bacillus subtilis ( Enterococcus ) Entericococcus is one or more bacteria or their cultures selected from the group consisting of, and enterococcus that can inhibit the growth of decay bacteria by inducing a decrease in pH faecium ) and Enterococcus faecalis may be used by mixing one or more lactic acid bacteria or their cultures selected from the group consisting of, even more preferably all of the five bacteria described above or a culture thereof. Can be used.

In step 2), the lactic acid bacteria (primary bacteria) that can be used are not particularly limited, and lignin and cellulose may be converted into low molecular sugars, lactates, and the like at a high concentration of organic substances present in the leachate prepared in step 1). Lactobacillus plant tarum, a lactic acid bacterium that can decomposeLactobacillus plantarum), Lactobacillus casei (Lactobacillus casei), Lactobacillus lactis (Lactobacillus lactis) And Lactobacillus brevis (Lactobacillus brevisIt may be one or more uniform selected from the group consisting of. In particular, the bacteria are inoculated in the desorption solution prepared in step 1) to induce pH lowering when fermentation (politics) fermentation, inhibit the growth of decayed bacteria derived from food waste to improve the occurrence of severe odor that is a problem of pure anaerobic fermentation It works.

In step 2), the usable photosynthetic bacteria (secondary bacteria) are not particularly limited, and Rhodopseudomonas is a photosynthetic bacterium that has a strong deodorizing effect in the upper and lower portions of the fermenter after the fermentation (politics) fermentation. palustris ), Rhodobacter sphaeroides ) and Rhodobacter capsulatus can be one or more homogeneous selected from the group consisting of.

In particular, the inoculation sequence of lactic acid bacteria (primary bacteria) and photosynthetic bacteria (secondary bacteria) in step 2) is closely related to the odor reduction effect of the produced seed solution, for example, inoculated with photosynthetic bacteria after lactic acid bacteria inoculation. In one case, the odor intensity of the spawn fluid is detected odor (a condition that can be felt but it is not known what smell), or conversely, when lactic acid bacteria are inoculated after inoculation with photosynthetic bacteria, a strong odor is easily detected. Because it is a state of being enough, for example, the degree to which cresol can be smelled in a hospital, or an unbearable odor (a state where breathing is stopped by the intense smell which is hard to endure), it produces odor reduction spawn liquid from desorption liquid It is essential to inoculate photosynthetic bacteria after inoculation with lactic acid bacteria in the desorption solution.

In step 2), the method of contacting the ligation liquid with lactic acid bacteria (primary bacteria) or cultures thereof, and photosynthetic bacteria (secondary bacteria) or cultures thereof is not particularly limited, and the stripping solution prepared from step 1) is used. When mixed with the lactic acid bacteria and photosynthetic bacteria to effectively multiply, and can be mixed as a ratio that significantly reduces the odor intensity of the produced spawn liquid, preferably, the culture solution mixed with the desorption liquid and one or more lactic acid bacteria 90:10 to 99 A volume ratio of 1: 1, more preferably, a volume ratio of 95: 5 to 99: 1, and a culture medium in which the at least one lactic acid bacterium mixture and the at least one photosynthetic bacterium are mixed in a volume ratio of 90:10 to 99: It may be mixed at a volume ratio of 1, more preferably at a volume ratio of 93: 7 to 97: 3.

Through this method, the present invention provides a supernatant obtained from a culture solution obtained by sequentially contacting lactic acid bacteria (primary bacteria) or cultures thereof, and photosynthetic bacteria (secondary bacteria) or their cultures with a detachment solution prepared from food waste. A odor reduction seed solution comprising a was prepared.

Third, an object of the present invention is to prepare a composition for hardly degradable wastewater treatment or wastewater purification from the prepared seed solution.

To this end, in the present invention

1) preparing a stripping solution from food waste;

2) culturing the sequencing solution and lactic acid bacteria (primary bacteria) or culture thereof, and photosynthetic bacteria (secondary bacteria) or culture thereof in a sequential contact with a volume ratio of 90:10 to 99: 1;

3) A seed solution obtained by precipitating the cultured broth to obtain a supernatant and Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) comprising the step of culturing by contacting at least one bacterium selected from the group consisting of or a culture thereof, and the non-degradable wastewater in a volume ratio of 50:25:25 to 70:15:15 To provide a method for producing a composition for hardly degradable wastewater treatment or wastewater purification using a malodor reducing seed solution.

In the method for preparing the composition, the bacterium inoculating food waste during preparation of the desorption liquid of step 1) is not particularly limited, and the high concentration of organic and inorganic substances contained in the food waste is rapidly hydrolyzed to solidify the volume of the solids. Reduce the carbon dioxide and / or nitrogen gas, and synthesize or secrete sugars, amino acids and vitamins to inoculate the lactic acid bacteria in a subsequent process step, i.e. Microorganisms that can provide, preferably Enterococcus faecium ), Enterococcus faecalis , Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) may be used at least one or a culture thereof selected from the group consisting of,

More preferably, Saccharomyces ( Saccharomyces) having an initial decomposition capacity in contact with food waste of the above-described bacteria cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Enterococcus ) Entericococcus is one or more bacteria or their cultures selected from the group consisting of, and enterococcus that can inhibit the growth of decay bacteria by inducing a decrease in pH faecium ) and Enterococcus faecalis may be used by mixing one or more lactic acid bacteria or their cultures selected from the group consisting of, even more preferably all of the five bacteria described above or a culture thereof. Can be used.

In step 2), the usable lactic acid bacteria (primary bacteria) is not particularly limited, and lignin and cellulose may be used to convert the high concentration organic materials present in the desorption liquid prepared in step 1) into low molecular sugars and lactates. Lactobacillus Lactobacillus is a lactic acid bacterium that can decompose the back plantarum ), Lactobacillus casei ), Lactobacillus lactis ) and Lactobacillus brevis ( Lactobacillus brevis ) may be one or more homogeneous selected from the group consisting of. In particular, the bacteria are inoculated in the desorption solution prepared in step 1) to induce the pH lowering when fermentation (politics) fermentation, inhibit the growth of decayed bacteria derived from food waste to improve the occurrence of severe odor, which is a problem of pure anaerobic fermentation It works.

In step 2), the usable photosynthetic bacteria (secondary bacteria) are not particularly limited, and Rhodopseudomonas is a photosynthetic bacterium that has a strong deodorizing effect in the upper and lower portions of the fermenter after the fermentation (politics) fermentation. palustris ), Rhodobacter sphaeroides ) and one or more homogeneous selected from the group consisting of Rhodobacter capsulatus .

In step 2), the method of contacting the ligation liquid with lactic acid bacteria (primary bacteria) or cultures thereof, and photosynthetic bacteria (secondary bacteria) or cultures thereof is not particularly limited, and the stripping solution prepared from step 1) is used. When mixed with the lactic acid bacteria and photosynthetic bacteria to effectively multiply, and can be mixed as a ratio that significantly reduces the odor intensity of the produced spawn liquid, preferably, the culture solution mixed with the desorption liquid and one or more lactic acid bacteria 90:10 to 99 A volume ratio of 1: 1, more preferably 95: 5 to 99: 1, and a mixing ratio of the at least one lactic acid bacterium and the at least one photosynthetic culture medium mixed with a culture solution of 90:10 to 99: 1 The volume ratio, more preferably, may be mixed at a volume ratio of 93: 7 to 97: 3.

As a bacterium inoculated into the seed solution obtained in step 2) in the preparation of the composition of step 3), microorganisms capable of rapidly decomposing the hardly decomposable substance contained in the hardly degradable wastewater, for example, the Pseudomonas footida ( Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) may be used one or more bacteria selected from the group consisting of or a culture thereof, more preferably all of the two bacteria described above or a culture thereof may be used, In addition, any bacteria capable of decomposing the hardly decomposable substance can be used.

The term "hard-degradable wastewater" refers to organic polluted wastewater that is difficult to be decomposed by microorganisms, and may be classified into a case in which the polluted organic material is hardly decomposable and a case in which the degradable organic wastewater contains toxic substances that inhibit the degradation activity of microorganisms. Can be. The degradability of microorganisms is based on enzymatic reactions, which is why they are difficult to decompose if enzymes necessary for degradation are not ready or xenobiotic among natural microorganisms, as in synthetic materials. ) And a large molecule having a high molecular weight, which is difficult to transport into a microorganism. Non-degradable wastewaters derived from toxics include insecticides, herbicides, halogenated solvents, other organic solvent-containing wastewaters, heavy metal-containing wastewaters, and cyanide-containing wastewaters, and double insecticides, herbicides, halogenated solvents, and heavy metal-containing wastewaters. can be classified as xenobiotic. In addition, wastewater containing humic substances such as wastewater containing synthetic resin components or some landfill leachate may be classified as polymeric hardly degradable wastewater.

The types of hardly degradable wastewater may include hydrocarbon or chlorinated hydrocarbon wastewater, dyeing wastewater, leather wastewater and electronic product manufacturing facility wastewater.

Wastewater containing hydrocarbons or chlorinated hydrocarbons includes aromatic hydrocarbons widely used in the field of organic chemical industry, phenol widely used in petroleum refinery, petrochemical industry or phenolic resin factory, and naphthalene widely used in petroleum related product production plants. Hardly degradable properties such as benzene-based materials, chlorobenzene materials, chlorophenol materials and halogenated aliphatic hydrocarbons (ex. Trichloroethylene, tetrachloroethylene, trichloroethane, dichloromethane, trichloromethane, tetrachloromethane) Contains substances

The dyeing wastewater contains hardly decomposable substances such as dyeing compounds used in the dyeing industry and synthetic detergents such as polyvinyl alcohol (PVA).

The leather wastewater contains hardly decomposable substances such as preservatives used to prevent corruption of the leather.

The wastewater discharged from the electronics manufacturing facility contains hardly decomposable substances such as paints and pigment compounds used for surface treatment in the plating process of the electronics manufacturing facility.

In step 3), the method of contacting the spawn solution, the hardly degradable wastewater decomposing bacteria or the culture thereof, and the hardly decomposable wastewater is not particularly limited, and the decomposition of the hardly decomposable substance upon mixing with the spawn solution prepared in step 2). When the bacteria are effectively propagated and mixed with the actual hardly degradable wastewater, the hardly degradable wastewater decomposing bacteria can be mixed in a ratio that can effectively adapt the wastewater and decompose the hardly decomposable substances contained in the wastewater as soon as possible. Preferably, the spawn solution, the culture medium containing one or more of the hardly degradable wastewater decomposing bacteria, and the hardly degradable wastewater have a volume ratio of 50:25:25 to 70:15:15, more preferably 55: 22.5: 22.5 to 65: 17.5: May be mixed at a volume ratio of 17.5.

In particular, the method for preparing the composition is to prepare a desorption liquid from food waste through a series of process steps made continuously, to produce a malodor reducing seed solution from the prepared desorbing liquid, and to treat the degradable wastewater or It may be to prepare a composition for waste water purification (Fig. 1 and Fig. 2).

Through this method, the present invention is a seed solution obtained from a culture solution obtained by cultivating the desorption solution prepared from food waste sequentially by contacting lactic acid bacteria (primary bacteria) or its culture, and photosynthetic bacteria (secondary bacteria) or its culture. Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) was prepared a composition for hardly degradable wastewater treatment or wastewater purification comprising a culture medium in contact with one or more bacteria or its culture selected from the group consisting of SCK) and the hardly degradable wastewater.

Fourth, an object of the present invention is to provide a hardly degradable wastewater treatment or wastewater purification method using the prepared composition.

To this end, in the present invention, the prepared composition in an amount of 50 to 200 kg / day in waste water of 1,000 to 2,500 tons, preferably in an amount of 100 to 150 kg / day, 1 to 15 days, preferably It is preferable to perform the contact for 7 days to 10 days. This is because when the composition is out of the content range and period of action, the composition cannot decompose the hardly decomposable substance contained in the hardly decomposable wastewater at maximum efficiency compared to the hardly decomposable wastewater (FIGS. 4 to 6).

As described above, the composition for the non-degradable wastewater treatment or wastewater purification according to the present invention can not only use environmentally friendly food waste degreasing solution, which is an important factor of water pollution and marine pollution, but also to improve the function of hardly degradable wastewater treatment or wastewater purification. It can be usefully used.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the invention, the present invention is not limited by the following examples.

[ Example ]

Example  1: Manufacture of Talisolate from Food Waste

1-1: Inoculation strain

As a strain inoculating food waste, Enterococcus was isolated from calf feces in this embodiment ( Enterococcus) faecium ) and Enterococcus faecalis), my process to a non - celebrity saccharide separated from the alcohol (Saccharomyces cerevisiae), Candida utility less (Candida utilis) was separated from the feed, and was separated from the soybean Bacillus subtilis (Bacillus subtilis ) bacteria were used.

1-2: strain culture medium and culture conditions

The medium and culture conditions used to efficiently enrich the strain prepared in Example 1-1 before inoculating food waste are as follows:

Enterococcus faecium ) and Enterococcus faecalis ) culture medium was prepared by adding 100 g of skim milk, 100 ml of tomato juice, and 5 g of yeast extract to 1 L of distilled water, and then agitating and sterilizing 100 ml in a 500 ml glass bottle. .

Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis subtilis ) culture medium was prepared by adding 3 g of Yeast extract, 3 g of Malt extract, 5 g of Peptone, and 10 g of Dextrose to 1 L of distilled water, and then agitating 100 ml into a 500 ml glass bottle and sterilizing them.

Enterococcus prepared in Example 1-1 in 100 ml of the culture medium thus prepared ( Enterococcus faecium ), Enterococcus faecalis ), Saccharomyces cerevisiae ), Candida utilis , and Bacillus subtilis subtilis ) bacteria were inoculated and shaken incubated at 25 ° C. for 2 days to prepare single strain cultures. Mixed strain culture was prepared by mixing the single strain culture in a 1: 1 ratio.

1-3: desorption solution preparation and Extraction rate

In order to prepare a detachment solution by inoculating a single strain culture medium or a mixed strain culture medium prepared in Example 1-2 to food waste, in this embodiment, 100 kg of food waste collected from homes, restaurants, and community service centers was dispensed by Example. Single strain culture medium or mixed strain culture solution prepared in 1-2 was inoculated sufficiently to 3 to 5% by weight to food waste and incubated for 3 days. The cultured food waste was first pulverized to a size of 4 to 5 cm with a grinder, and the first pulverized food waste was pulverized to a size of 20 mm again. The pulverized powder was centrifuged at 2,500 rpm for 20 minutes to receive a leachate. The process of preparing the detachment solution from the food waste is shown in FIG. 1 (FIG. 1), and the extraction rate of the detachment solution obtained from the food waste according to the inoculation of the single strain culture medium or the mixed strain culture solution is shown in FIG. 2 (FIG. 2).

As shown in FIG. 2, when inoculated with a single strain culture, Enterococcus faecium had an extraction rate of 49%, Enterococcus faecalis had an extraction rate of 52%, and Saccharomyces ceres. Saccharomyces cerevisiae ) showed 60% extraction rate, Candida utilis 64% extraction rate, and Bacillus subtilis extraction rate 71% (Fig. 2). When inoculated with the mixed strain culture solution containing all, it showed a 77% extraction rate of the detachment (Fig. 2).

On the other hand, when the strain was not inoculated, it showed the lowest extract rate of 40% (Figure 2). This means that the mixed strain culture medium has a significantly higher degrading power of organic matter, cellulose, and fats present in the food waste than the non-inoculated and single strain culture medium.

Example  2: from sorbent Odor reduction Spawn  Produce

2-1: inoculation strain

As strains inoculated into the desorption solution of Example 1, lactic acid bacteria (primary bacteria) and photosynthetic bacteria (secondary bacteria) were used in this example, respectively.

The lactic acid bacteria (primary bacteria) is Lactobacillus plantarum ( Lactobacillus ) isolated from nature Lactobacillus casei (Lactobacillus plantarum isolated from), kimchi casei ), Lactobacillus lactis ) and Lactobacillus brevis ) bacteria were used.

The photosynthetic bacteria (secondary bacteria) are Rhodopseudomonas isolated from eutrophicated pond palustris ), Rhodobacter sphaeroides and Rhodobacter capsulatus ) bacteria were used.

2-2: Strain Culture Media and Culture Conditions

The medium and culture conditions used to efficiently enrich the strain prepared in Example 2-1 before inoculating the lysate of Example 1 were as follows:

Lactobacillus plantarum ), Lactobacillus casei ), Lactobacillus lactis ) and Lactobacillus brevis) a medium for the culture of bacteria is a Enterococcus passive help (Enterococcus used in Examples 1-2 faecium ) and Enterococcus faecalis ) was prepared in the same manner as the culture medium.

Lactobacillus plantarum prepared in Example 2-1 in 100 ml of the medium prepared as described above ( Lactobacillus plantarum ), Lactobacillus casei ), Lactobacillus lactis ) and Lactobacillus brevis ) bacteria were inoculated and shaken incubated at 25 ° C. for 2 days to prepare a single strain culture. Mixed strain culture was prepared by mixing the single strain culture in a 1: 1 ratio.

In addition, Rhodopseudomonas palustris ), Rhodobacter sphaeroides ) and Rhodobacter capsulatus culture medium were cultured with 1 L of rice water, 2.5 g of Malic acid, 20 g of brown sugar, (NH ₄ ) 2SO ₄ 1.25 g, MgSO ₄ · 7H ₂ O 0.2g, CaCl _{2 · 2H 2 O 70 mg,} Ferric citrate 10 mg, KH 2 PO 4 0.6 g, K 2 HPO 4 0.9 g, MnSO 4 · H 2 O 20 mg, CuSO 4 · 5H20 and then stirred for 1 mg was added, NaOH Using to adjust the pH to 6.5 ~ 7 conditions, and then dispensed by 100 ml in 500 ml glass bottle, was prepared by filtration and sterilization.

Rhodopseudomonas prepared in Example 2-1 in 100 ml of the medium prepared as described above palustris ), Rhodobacter sphaeroides ) and Rhodobacter capsulatus ) were inoculated respectively and shaken incubated in a 25 ° C. incubator with a white blood lamp for 7 days to prepare a single strain culture medium. Mixed strain culture was prepared by mixing the single strain culture in a 1: 1 ratio. At this time, since the Rhodoshudomonas palustris bacteria were aerobic photosynthetic bacteria, 500 ml glass bottles, which were culture vessels, were incubated with a breathable silicone stopper, and the Rhodobacter sphaeroides and Rhodobacter capsularus bacteria were anaerobic photosynthetic bacteria, 500 ml. The glass bottles were incubated with rubber stoppers.

2-3: Odor reduction Spawn  Manufacturing and Deodorizing Effect Analysis

In order to inoculate the single strain culture medium or the mixed strain culture solution prepared in Example 2-2 to the desorption solution obtained in Example 1-3, the odor reducing seed solution was prepared in this Example. It is transferred to the fermentation tank, and the single strain culture medium or mixed strain culture solution of lactic acid bacteria (primary bacteria) prepared in Example 2-2 is mixed at a volume ratio of 99: 1, and is 27-30 ° C., pH 6-7. It was fermented for 2 days under conditions. In order to deodorize the odor generated from the stationary fermentation stripping solution, the stationary fermentation stripping solution is transferred to an incandescent deodorizing tank, and a single strain of the photosynthetic bacteria (secondary bacteria) prepared in Example 2-2 is prepared. The culture solution or mixed strain culture solution was mixed at a volume ratio of 95: 5, and cultured at 30 to 32 ° C. and pH 5 to 6 for 3 days.

The culture broth was centrifuged at 2,500 rpm for 20 minutes to receive the seed solution. The process for preparing the seed solution from the desorption solution is shown in FIG. 3 (FIG. 3), and the single strain culture medium or mixed strain culture solution of lactic acid bacteria (primary bacteria), and the single strain culture medium or mixed strain culture medium of photosynthetic bacteria (secondary bacteria) The odor reduction effect of the seed solution prepared according to the inoculation and process sequence of the is shown in Table 2 (Table 2).

Odor test and acceptance criteria are as follows.

That is, five normal people were selected as the odor judge, and the odor detected by many of the odor detected by the odor judge was determined as a criterion. The odor test and the criterion of the odor were determined by the National Institute of Environmental Research shown in Table 1 below. It was performed according to the test method (Table 1).

Odor Odor intensity Explanation 0 Odorless Relative odorless condition that detects nothing with normal smell One Detection Threshold I don't know what it smells but I can feel something 2 Moderate The state that can distinguish what smell 3 Strong odor Easily detectable condition (ex. How scented cresol can be in the hospital) 4 Extreme strong odor Very strong (ex. The smell of a conventional restroom) 5 Over strong odor A condition that seems to stop breathing due to an intense smell that is unbearable

Odor intensity The number of odor judges who agreed with the item A B C D E Odorless Detection odor 5 Moderate odor One Strong odor 2 3 3 Unbearable odor 5 3 One 2

(A: unstrained leachate,

B: the detachment solution of Examples 1-3,

C: The lysate obtained by inoculating the lysate of Example 1-3 with the mixed strain culture solution of the lactic acid bacteria (primary bacterium) of Example 2-2, followed by stationary fermentation,

D: Inoculated with the culture strain of lactic acid bacteria (primary bacteria) of Example 2-2 to the desorption solution of Example 1-3 and inoculated with the culture strain of photosynthetic bacteria (secondary bacteria) to the freely fermented liquor solution. One spawn solution, and

E: Inoculated with the culture strain cultured by inoculating the mixed strain culture medium of the photosynthetic bacteria (secondary bacteria) of Example 2-2 to the desorption solution of Example 1-3, and inoculating the mixed strain culture medium of the lactic acid bacteria (primary bacteria) Spawn solution)

As shown in Table 2, in the case of the unstrained leachate (A), all five malodorous judges were in favor of unbearable odor, and in the case of the leachate of Example 1-3 (B), two odors were observed. And 3 people were in favor of unbearable odor, and in the case of the desorption solution inoculated with the mixed strain culture of lactic acid bacteria (primary bacteria) (C), 3 were in strong odor, 1 in unbearable odor and 1 in normal odor. . On the other hand, in the case of the seed solution obtained by sequentially inoculating lactic acid bacteria (primary bacteria) and photosynthetic bacteria (secondary bacteria) according to the process of FIG. 3 (D), all five odor judges were approved for detection odor. From these results, it can be seen that when inoculated and cultured in the order of lactic acid bacteria (primary bacteria) and photosynthetic bacteria (secondary bacteria) in the desorption solution, it is possible to effectively deodorize the odor generated in the desorption solution.

In addition, in order to investigate the functions of lactic acid bacteria (primary bacteria) and photosynthetic bacteria (secondary bacteria) that affect the odor reduction of the desorption solution, the order of inoculation of lactic acid bacteria (primary bacteria) and photosynthetic bacteria (secondary bacteria) was changed. As a result, it was confirmed that the deodorizing effect of the seed solution (E) obtained therefrom is significantly reduced compared to the seed solution (D) does not change the order (Table 2). In other words, three people with strong odors and two people with unbearable odors agreed. This means that photosynthetic bacteria perform a function of decomposing the generated malodorous substances rather than suppressing the production of malodorous substances.

Example  3: Preparation of a hardly degradable wastewater treatment or wastewater purification composition

3-1: inoculation strain

Embodied as a strain inoculated into the seed solution of Example 2-3, in the present embodiment is a Pseudomonas Footy separated from textile dyeing plant waste water (Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) was used to degrade the degradable bacteria.

3-2: Strain Culture Media and Culture Conditions

The medium and culture conditions used to efficiently enrich the strain prepared in Example 3-1 before inoculating the seed solution of Example 2-3 were as follows:

Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) bacteria culture medium was prepared by adding 3 g of Yeast extract, 5 g of Peptone, and 5 g of NaCl to 1 L of distilled water, followed by stirring.

Pseudomonas Footy prepared in Examples 3-1 in 100 ml of the culture medium thus prepared is (Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) bacteria were inoculated respectively and shaken culture at 37 ° C. for 2 days to prepare a single strain culture. Mixed strain culture was prepared by mixing the single strain culture in a 1: 1 ratio.

3-3: Preparation of Biodegradable Wastewater Treatment or Wastewater Treatment Purification and Biochemical Characterization

In order to prepare a composition capable of effectively treating or purifying hardly degradable wastewater, in this embodiment, the single strain culture medium or mixed strain culture solution of Example 3-2 and the seed culture solution of Example 2-3 stored at 10 ° C. or lower. , And non-degradable wastewater (ex. Fiber dyeing wastewater, fiber processing wastewater, electronic wastewater, chemical wastewater, etc.) in a volume ratio of 6: 2: 2, and then incubated for 2 days at 25-30 ° C. and pH 7 conditions. To obtain a composition for hardly degradable wastewater treatment or wastewater purification.

In addition, in the present embodiment, the biochemical characteristics, for example, physicochemical characteristics, viable cell number, biological safety, and heavy metal content of the releasing solution of Example 1 and the seed solution of Example 2, as well as the composition prepared above are analyzed It was.

<Physical Characteristic Analysis>

The physicochemical characteristics of the detachment solution of Example 1, the seed solution of Example 2, and the composition of Example 3 were analyzed. Biological oxygen demand (BOD), chemical oxygen demand (COD), total nitrogen concentration, total phosphorus concentration, salinity, and odor intensity were examined as items indicating physicochemical characteristics (Table 3).

Item Desorption Spawn Composition density unit density unit density unit Biological Oxygen Demand (BOD) 66,000 ppm 1,750 ppm 1,650 ppm Chemical oxygen demand (COD) 5,300 ppm 1,593 ppm 990 ppm Total nitrogen 41,000 ppm 1,836 ppm 930 ppm Total phosphate 2,300 ppm 137 ppm 120 ppm Salinity 0.77 % 0.75 % 0.6 % Odor intensity Unbearable odor Detection odor Detection odor

As shown in Table 3, the spawn solution and the composition showed significantly lower concentration values in all items compared to the detachment solution, and it was confirmed that the odor intensity was also significantly reduced (Table 3). In particular, the composition showed a bad smell strength similar to the seed solution, which means that the deodorizing action of photosynthetic bacteria is also effective in the manufacturing process of the composition.

<Survival Cell count  Analysis>

In the case of the composition used for the hardly degradable wastewater treatment or the wastewater purification, the viable cell count of the composition plays a very important role in the efficiency of the actual hardly degradable wastewater treatment or the wastewater purification. , And the composition of Example 3 were mixed with the hardly degradable wastewater (sample), respectively, to determine the viable cell count. The measured viable cell numbers are shown in Table 4 (Table 4). The viable cell count was measured three times according to the general bacterial count test method of the drinking water quality test method, and the result was averaged (Ministry of Environment, 2007, Drinking water quality test method).

Type of sample Number of viable cells (horses / ml) Desorption Spawn Composition Textile Dyeing Wastewater, Textile Processing Wastewater 5.2 X 10 ⁶ 5.2 X 10 ⁸ 9 X 10 ⁸ Electronic Wastewater, Chemical Wastewater 9.1 X 10 ⁸

As shown in Table 4, the number of viable cells in the composition mixed with the sample was the highest, followed by the number of viable cells in the seed solution and the number of viable cells in the desorption solution (Table 4). This means that not only the reduction of organics and inorganics but also the growth of cells occurred during the process of preparing the composition from the leachate.

<Biological safety investigation>

Carried out in Example 2 to examine the biological safety of the composition of the seed mixture and the third embodiment, the original pathogenic microorganisms in the food garbage is likely to exist in the proliferation of the manufacturing process of the liquid and the seed composition Salmonella (Salmonella sp . ), Shigella sp. , And E. coli group, which are used as hygiene indicators, were measured. The detection results of the microorganisms are shown in Table 5 (Table 5). The detection of Salmonella and Shigella was measured according to the Infectious Disease Testing Laboratory Guidelines (National Institute of Health, 2007, Infectious Disease Laboratory Testing Guidelines), and the detection of E. coli group was measured according to the wastewater testing method in the Waste Management Guidelines (Ministry of Environment, 2007, Waste management guidelines).

Inspection items result unit Spawn Composition Salmonella sp . Bacteria voice voice - Shigella sp . Bacteria voice voice - E. coli group voice voice -

As shown in Table 5, the spawn solution and the composition made from the food waste stripping liquid are all biologically safe because no Salmonella, Shigella and E. coli groups are detected (Table 5).

<Heavy metal concentration survey>

The heavy metal concentrations of the lead (Pb), arsenic (As) and cadmium (Cd) contained in the seed solution of Example 2 and the composition of Example 3 are shown in Table 6 (Table 6). The concentration of heavy metals was measured according to the method for measuring trace contaminants in the environment (Ministry of Environment, 2007, Test method for trace contaminants in the environment).

Inspection items result unit Spawn Composition Pb 0.04 0.03 ppm Arsenic (As) 0.07 0.02 ppm Cadmium (Cd) 0.005 0.003 ppm

As shown in Table 6, the concentrations of lead, arsenic, and cadmium in the spawn solution and composition are all equal to the drinking water quality standards of Korea (lead is 0.05 ppm or less, arsenic is 0.05 ppm or less, and cadmium is 0.005 ppm or less) or It was confirmed that it was detected lower (Table 6). From these results, it can be seen that the seed liquid and the composition of the present invention have a very high safety content as the content of the heavy metal is less than or equal to the natural detection amount of the heavy metal detected in nature.

Example  4: Efficacy analysis of the composition against hardly degradable wastewater

In order to investigate whether the composition prepared in Example 3 effectively treats or purifies the actual hardly degradable wastewater, the present embodiment targets the efficacy of the composition, that is, wastewater treatment, at a wastewater treatment plant in a textile dyeing (processing) factory in Gumi, Gyeongsangbuk-do. The chemical oxygen demand (COD), total nitrogen concentration, and total phosphorus concentration in the effluent discharged from the process were measured, respectively. For company A, the wastewater treatment plant is 2,000 tons / day, and for company B, the wastewater treatment plant is 1,500 tons / day. As the experimental group, the group in which the composition of Example 3 was administered 100 kg daily for 15 days to the wastewater treatment tank (aeration tank) of the wastewater treatment plant was set as the control group, respectively.

4-1: Chemical oxygen demand ( COD ) change

The chemical oxygen demand (COD) in the effluent discharged from the wastewater treatment process of Example 4 was measured in accordance with the water pollution process test method chemical oxygen demand measurement method (Ministry of Environment, 2007, drinking water quality process test method). Figure 4 is a graph showing the change in chemical oxygen demand in the effluent according to the number of days of administration of the composition (Fig. 4).

As shown in FIG. 4, in the case of wastewater of company A, the chemical oxygen demand of the effluent before the administration of the composition was 56 ppm, and continuously decreased after administration of the composition, and maintained at 29 ppm after 10 days before administration of the composition. When compared with the chemical oxygen demand of the actual waste water it was confirmed that the reduction of about 34.1% (Fig. 4). On the other hand, in the case of the control group not administered the composition, the chemical oxygen demand was 48 ppm even after 10 days of administration, and it was confirmed that the effect of reducing the natural chemical oxygen demand was extremely weak (FIG. 4).

For company B wastewater, the chemical oxygen demand of the effluent before administration of the composition was 166 ppm, and it was continuously decreased after administration of the composition, and remained at 61 ppm after 10 days of administration, In comparison, it was confirmed that the 73.2% reduction (Fig. 4). On the other hand, in the case of the control group not administered the composition, the chemical oxygen demand was 120 ppm after 10 days of administration, and it was confirmed that the effect of reducing the natural chemical oxygen demand was extremely weak (FIG. 4).

Therefore, the composition of the present invention significantly reduced the chemical oxygen demand of the fiber dye (processed) wastewater, and especially the wastewater with a high initial chemical oxygen demand was found to have a better reduction effect.

4-2: total nitrogen ( Total nitrogen ) Change in concentration

In order to measure the total nitrogen concentration in the effluent discharged from the wastewater treatment process of Example 4, the total nitrogen measurement method in the water pollution process test method using the same sample as the sample taken when measuring the chemical oxygen demand of Example 4-1 The total nitrogen concentration was measured according to (Ministry of Environment, 2007, Drinking Water Quality Process Test Method). Figure 5 is a graph showing the change in total nitrogen concentration in the effluent with the number of days of administration of the composition (Figure 5).

As shown in FIG. 5, in the case of the wastewater of company A, the total nitrogen concentration of the effluent before administration of the composition was 5.6 ppm, and continuously decreased from 4 days after the administration of the composition to remain below 1.9 ppm after 7 days of administration. As compared to the total nitrogen concentration of the actual wastewater before administration of the composition was confirmed that the reduction of about 65.3% (Fig. 5). On the other hand, in the case of the control group not administered the composition, the total nitrogen concentration was 4.9 ppm even after 10 days of administration, and it was confirmed that the effect of reducing the natural total nitrogen concentration was extremely weak (FIG. 5).

For Company B wastewater, the total nitrogen concentration of the effluent before administration of the composition was 33.5 ppm, and it was continuously reduced after administration of the composition, remaining at 20.1 ppm after 8 days of administration, It was confirmed that the reduction of about 37.5% when compared (Fig. 5). On the other hand, in the case of the control group not administered the composition, the total nitrogen concentration was 30 ppm after 10 days of administration, and it was confirmed that the effect of reducing the natural total nitrogen concentration was extremely weak (FIG. 5).

Therefore, it can be seen that the composition of the present invention significantly reduces the total nitrogen concentration of the fiber dye (processed) wastewater.

4-3: Total phosphorus ( Total phosphate ) Change in concentration

In order to measure the total phosphorus concentration in the effluent discharged from the wastewater treatment process of Example 4, the total phosphorus measurement method in the water pollution test method using the same sample as the sample taken when measuring the chemical oxygen demand of Example 4-1 The total phosphorus concentration was measured according to the Ministry of Environment, 2007, Drinking Water Quality Process Test Method. 6 is a graph showing the change in total phosphorus concentration in the effluent with the number of days of administration of the composition (FIG. 6).

As shown in FIG. 6, in the case of the wastewater of company A, the total phosphorus concentration of the effluent before the administration of the composition was 0.5 ppm, and the composition was continuously maintained after the administration of the composition and maintained below 0.1 ppm after 8 days of administration. When compared with the total phosphorus concentration of the actual wastewater was confirmed to be reduced by 78.1% (Fig. 6). On the other hand, in the case of the control group not administered the composition, the total phosphorus concentration was 0.4 ppm even after 10 days of administration, and it was confirmed that the effect of reducing the natural total phosphorus concentration was extremely weak (FIG. 6).

For Company B wastewater, the total phosphorus concentration in the effluent prior to administration of the composition was 1.7 ppm, which continued to decrease from day 4 after the composition was administered and remained at 1.2 ppm after 8 days of administration, while the total amount of actual wastewater before administration of the composition When compared with the phosphorus concentration was confirmed to be reduced by about 29.1% (Fig. 6). On the other hand, in the case of the control group not administered the composition, the total phosphorus concentration was 1.6 ppm after 10 days of administration, and it was confirmed that the effect of reducing the natural total phosphorus concentration was extremely weak (FIG. 6).

Thus, the composition of the present invention significantly reduces the total phosphorus concentration of the fiber dye (processed) wastewater, and particularly shows a better reduction effect in wastewater containing low concentrations of phosphorus.

1 shows a process for preparing a detachment solution from food waste according to one embodiment of the present invention.

Figure 2 is a graph showing the extraction rate of the desorption solution obtained from food waste according to the inoculation of a single strain culture medium or mixed strain culture medium according to an embodiment of the present invention.

Figure 3 shows a process for producing a malodor reducing seed solution from the desorption solution according to an embodiment of the present invention.

Figure 4 is a graph showing the change in chemical oxygen demand (COD) in the wastewater according to the number of days of administration of the composition for the treatment of difficult-degradable wastewater treatment or wastewater purification according to an embodiment of the present invention.

5 is a graph showing a change in the total nitrogen concentration in the wastewater according to the number of days of administration of the composition for treatment or wastewater purification of difficult-degradable wastewater according to an embodiment of the present invention.

Figure 6 is a graph showing the change in the total phosphorus concentration in the wastewater according to the number of days of administration of the composition for the treatment of difficult-degradable wastewater or wastewater purification according to an embodiment of the present invention.

Claims (14)

1) Enterococcus in garbage Caucus passive help (Enterococcus faecium ), Enterococcus faecalis), saccharose in my process serenity non - (Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) contacting two or more bacteria selected from the group consisting of or a culture thereof; And 2) The culture medium is crushed and centrifuged to obtain a supernatant to obtain a desorption solution, the method for producing a desorption solution from food waste. According to claim 1, characterized in that in step 1) the food waste and two or more bacteria or their culture in contact with 90:10 to 99: 1% by weight. 1) preparing a stripping solution from food waste; 2) culturing the sequencing solution and the lactic acid bacteria or the culture thereof, and photosynthetic bacteria or the culture thereof in a sequential contact with a volume ratio of 90:10 to 99: 1; And 3) Precipitating the culture broth to obtain a supernatant to obtain a supernatant, odor reduction seed production method. The desorption solution of claim 3, wherein a) Enterococcus in garbage Caucus passive help (Enterococcus faecium ), Enterococcus faecalis), saccharose in my process serenity non - (Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) contacting one or more bacteria selected from the group consisting of or a culture thereof; And b) the culture is obtained by crushing and centrifuging the culture and obtaining a supernatant. According to claim 3, wherein the lactic acid bacteria of step 2) Lactobacillus plantarum ), Lactobacillus casei ), Lactobacillus lactis ) and Lactobacillus brevis ( Lactobacillus brevis ) is one or more bacteria selected from the group consisting of, the manufacturing method. The method of claim 3, wherein the photosynthetic bacteria of step 2) Rhodopseudomonas palustris ), Rhodobacter sphaeroides ) and one or more bacteria selected from the group consisting of Rhodobacter capsulatus . A odor reducing seed solution comprising a supernatant obtained from a culture solution obtained by sequentially contacting a desorption solution prepared from food waste with a lactic acid bacterium or a culture thereof and a photosynthetic bacterium or a culture thereof. 1) preparing a stripping solution from food waste; 2) culturing the sequencing solution and the lactic acid bacteria or the culture thereof, and photosynthetic bacteria or the culture thereof in a sequential contact with a volume ratio of 90:10 to 99: 1; 3) A seed solution obtained by precipitating the cultured broth to obtain a supernatant and Pseudomonas putida ) and Enterobacter SCK ( Enterobacter At least one bacterium selected from the group consisting of SCK) or a culture thereof, and the step of contacting and incubating the hardly decomposable wastewater in a volume ratio of 50:25:25 to 70:15:15. Method for producing a composition for purification. The method of claim 8, wherein the desorption solution of step 1) a) Enterococcus in garbage Caucus passive help (Enterococcus faecium ), Enterococcus faecalis), saccharose in my process serenity non - (Saccharomyces cerevisiae), Candida utility less (Candida utilis ) and Bacillus subtilis ( Bacillus subtilis ) contacting one or more bacteria selected from the group consisting of or a culture thereof; And b) the culture is obtained by crushing and centrifuging the culture and obtaining a supernatant. The method of claim 8, wherein the lactic acid bacteria of step 2) Lactobacillus plantarum ), Lactobacillus casei ), Lactobacillus lactis ) and Lactobacillus brevis ( Lactobacillus brevis ) is one or more bacteria selected from the group consisting of, the manufacturing method. The method according to claim 8, wherein the photosynthetic bacteria of step 2) Rhodopseudomonas palustris ), Rhodobacter sphaeroides ) and one or more bacteria selected from the group consisting of Rhodobacter capsulatus . The manufacturing method according to claim 8, wherein the hardly degradable wastewater of step 3) is at least one wastewater selected from the group consisting of hydrocarbon or hydrocarbon chloride-containing wastewater, dyeing wastewater, leather wastewater and electronics manufacturing facility wastewater. The seed liquid resultant from the talriaek made from food waste lactic acid bacteria or a culture, and photosynthetic bacteria or a culture broth thereof contacting the culture in order, the Pseudomonas Footy (Pseudomonas putida ) and Enterobacter SCK ( Enterobacter SCK) 1 or more bacteria selected from the group consisting of, or a culture thereof, and a culture solution obtained by contacting the hardly degradable wastewater, the composition for hardly degradable wastewater treatment or wastewater purification. Refractory wastewater treatment or wastewater purification method, characterized in that the composition according to claim 13 in contact with the wastewater of 1,000 to 2,500 tons in an amount of 50 to 200 kg / day, for 1 to 15 days.

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