CN102849857B - Method for treating high-salinity high-concentration ammonia-nitrogen wastewater - Google Patents
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Abstract
The invention discloses a method for treating high-salinity high-concentration ammonia-nitrogen wastewater, characterized by: adopting sequencing batch reactor, accumulating nitrification sludge by alternately increasing the concentration of a nutrient solution or ammonia-nitrogen concentration, and domesticating to obtain high salinity-resisting high ammonia nitrogen-resisting nitrobacteria. According to the invention, the nitrobacteria can treat high-salinity wastewater having ammonia-nitrogen concentration of 500mg/L and having the salt content of no larger than 35g/L to make the effluent have the ammonia-nitrogen concentration of no larger than 10mg/L, thus the effluent achieve national level of discharging standard; the method has good effect on treating high-salinity high ammonia-nitrogen wastewater in chemical plant, leather factory, oil refinery and the like, can overcome the shortage that the nitrobacteria cannot simultaneously treat high-salinity and high ammonia-nitrogen wastewater existing in the prior art, and has important economic benefits and social benefits.
Description
Technical field
The present invention relates to a kind of wastewater treatment method, be specifically related to a kind of method by the anti-high salinity of cultivation, resisting high-concentration ammonia nitrogen nitrobacteria processing high salinity high ammonia-nitrogen wastewater, belong to the biological environmental production technical field.
Background technology
Nitrate pollution is one of major reason that causes body eutrophication, and nitrogenous compound is paid attention to widely and studied keeping water body cleaning and prevent that body eutrophication has vital role, having caused in recent years in the control water.Industries such as oil, chemical industry and chemical fertilizer efflux nitrogenous effluent, have Pollutant levels height, composition complexity, characteristics that quantity discharged is big as leather waste water, the qualified discharge difficulty is big; Particularly these waste water have the characteristics (saltiness is greater than 15g/L) of high salt mostly, cause Biodegradability of Wastewater poor, have brought big difficulty to improvement, cause sizable pressure to environment.
At present the treatment process at high salinity, high ammonia-nitrogen wastewater mainly contains physical method, chemical process, biological method etc., but owing to physics, the chemical method working cost is higher, treatment effect is undesirable, the while very easily causes secondary pollution to be difficult to promote in actual applications; Traditional biological method tool when handling low ammonia nitrogen waste water has great advantage, but when meeting the ammonia nitrogen in waste water excessive concentration, can suppress the metabolism of microorganism, thereby make microorganism lose degradation capability.And because condition restriction, can not carry out desalting treatment in sewage disposal as last, therefore in actual applications, screening salt tolerant high-effective microorganism provides the feasibility of biochemical degradation for the degraded of ammonia nitrogen.
Chinese patent, publication number is the treatment process that CN 101239751 A disclose a kind of high-concentration ammonia nitrogenous wastewater, it adopts intermittent activated sludge process, by improving the method for nutrient solution ammonia nitrogen concentration gradually, enrichment culture nitrobacteria from active sludge, can remove ammonia nitrogen in high density (greater than 500mg/L), but the nitrobacteria of institute's enrichment can't tolerate hypersaline environment, for high salt high ammonia-nitrogen wastewater, as leather waste water, treatment effect is relatively poor.
Chinese patent, publication number is that CN 101195507 A disclose the method that a kind of ammonia nitrogen waste water is handled, comprise carrier, nitrifier, the aerobic zone that the oxygen concentration gradient that utilizes diffusional resistance to produce in granule interior forms, oxygen-starved area and oxygen-starved area, form the environment that is fit to two processes combinations of nitrification and denitrification in carrier inside, carry out the oxidizing reaction of ammonia owing to the existence of oxygen on the granule sludge top layer, granule interior is because utilize the oxidation products of ammonia to carry out anti-nitration reaction under the anoxia condition, thereby realization single-stage biological nitrogen removal, realize nitrification and denitrification simultaneously, reached very high denitrification percent.But the nitrobacteria of institute's enrichment can't tolerate hypersaline environment, and for high salt high ammonia-nitrogen wastewater, as leather waste water, treatment effect is relatively poor.
Chinese patent, publication number is membrane sepn and the stripping combination treatment method that CN 101987767 A disclose a kind of high ammonia nitrogen and salinity RE waste water, its method steps is: after the Wastewater Pretreatment through disc filter to carrying out preliminary filtration treatment, enter the stripping device, behind the ammonia that recovery removes, enter membrane separation unit, wherein produce the water reuse, after concentrated solution is got back to back stripping device and reclaimed the ammonia remove, get back to the membrane sepn step again.Such technological advantage is: but the good direct reuse of effluent quality, but required cost is too high, and work-ing life is short, the manual operation difficulty.
The 26th the 2nd phase of volume of " environmental engineering " April in 2008, it is 800～1000mg/L, ammonia nitrogen 80～100mg/L that people such as Yu Deshuan, Zhang Yong delivers in " high salinity waste water sludge acclimatization and microorganism are studied mutually " literary composition at water inlet COD, under the condition of saltiness 17.5g/L, the effectively active sludge of degradation of ammonia nitrogen has been cultivated in domestication.But because the COD height, heterotrophic bacterium forms the competition inhibition to the nitrobacteria of autotrophy, and the nitrobacteria culture cycle is long, and proportion is low in active sludge.Simultaneously, the nitrobacteria of cultivating can't effectively be removed the waste water of high ammonia nitrogen.
The 3rd phase of Volume Four " environmental engineering journal " in March, 2010, Zhao Kaifeng, Wang Shuying etc. deliver in " the NaCl salinity is to the influence of salt tolerant activated sludge settling property and denitrogenation " literary composition the salt resistance ability of nitrifier are studied, when salinity is in 20 g/L scopes, ammonia nitrogen removal frank still can maintain more than 99%, the salt resistance ability that shows nitrifier is strong, but selected ammonia nitrogen concentration control is in 65 mg/L scopes, for not further research of high-concentration ammonia nitrogenous wastewater in its research.
Leather-making waste water has the characteristics of the high ammonia nitrogen of high salinity, generally adopts physico-chemical pretreatment and aerobic biological treatment combination process, but common nitrifier denitrogenation not only increases investment and land area, and the effluent quality ammonia nitrogen concentration is still very high.Biological reinforcing technology is in order to improve the processing power of Waste Water Treatment, and adds in this system from the dominant bacteria of occurring in nature screening or the composite bacteria by the generation of assortment of genes technology, to remove the method for a certain or a certain class objectionable impurities.At the characteristics of leather waste water high salinity, high-concentration ammonia-nitrogen, screening salt tolerant high efficiency nitrification bacterium carries out biological reinforced processing to waste water, and is significant in industrial production.
Summary of the invention
Goal of the invention: the objective of the invention is efficiently to handle the deficiency of high salt and high ammonia-nitrogen wastewater in order to solve prior art, a kind of nitrifier of adopting the batch activated sludge method domestication to cultivate anti-high salinity, high ammonia nitrogen is provided, and handle 500 mg/L concentration ammonia nitrogens, saltiness 35 g/L with interior high salinity waste water with the nitrobacteria of this anti-high salt, high ammonia nitrogen, the water outlet ammonia nitrogen concentration is reached in 10 mg/L, realize qualified discharge.
Technical scheme: in order to realize above purpose, the technical scheme that the present invention takes is:
The treatment process of a kind of high salinity, high-concentration ammonia nitrogenous wastewater, it may further comprise the steps:
Adopt batch activated sludge method, carry out enrichment by the method that improves nutrient solution ammonia nitrogen and salinity step by step, adopt NaCl, KCl simulation high salinity, initial salt concn is 5 g/L, final salt concn is 30～35 g/L, the ammonia nitrogen starting point concentration is 30～60mg/L, and ultimate density is 400～500mg/L, and COD concentration is 50mg/L～150mg/L; The enrichment culture of nitrobacteria is taked the mode that hockets, an enrichment culture cycle comprises two stages: the fs: the cultivation of salt tolerant nitrobacteria and enrichment, culture condition is: saltiness, with NaCl, KCl counts 5g/L, ammonia nitrogen concentration is 30～60mg/L, activated sludge concentration is 20～25g/L, temperature is 25 ~ 30 ℃, pH is that 7～8, DO is 4～6mg/L, and the fs does not add any additional carbon, domestication time fs is 2 to 4 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, enter subordinate phase: screening and the enrichment of the high efficiency nitrification bacterium of ammonia nitrogen in high density; The culture condition of subordinate phase is: saltiness, with NaCl, KCl counts 5g/L, ammonia nitrogen concentration 100～150mg/L, activated sludge concentration is 20～25g/L, 25 to 30 ℃ of temperature, and pH is 7～8, DO is 4～6mg/L, in subordinate phase, look zoogloea and form situation, add an amount of organic carbon, make COD concentration between 50mg/L～150mg/L, stimulate the heterotrophic bacterium growth with this, promote zoogloea to form, the subordinate phase domestication time is 4 to 6 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, finish subordinate phase, two stages have constituted an acclimation period; Improve the concentration of nutrient solution ammonia nitrogen and salinity step by step by the above-mentioned method that hockets, the enrichment of nitrobacteria is divided into 4 acclimation periods, making final salt concn is 30～35 g/L, final ammonia nitrogen concentration is 400～500mg/L, finally can screen and obtain handling ammonia nitrogen concentration 500 mg/L, saltiness 35 g/L with interior high salinity, high ammonia-nitrogen wastewater, the water outlet ammonia nitrogen concentration can reach 10 mg/L with interior anti-high salinity, the nitrobacteria of ammonia nitrogen in high density.
Active sludge of the present invention can be selected from the active sludge that contains nitrobacteria that adopt this area, the sewage work that preferably comes from efficient ammonia nitrogen removal ability, namely initial ammonia nitrogen is higher than 100mg/L, salinity is higher than the active sludge of ammonia nitrogen removal efficient more than 80% in the 5g/L waste water.
The treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, the mode that the enrichment culture of nitrobacteria is taked to hocket improves the concentration of nutrient solution ammonia nitrogen and salinity step by step, be total to 4 acclimation periods of enrichment culture, the initial salt concn of nutrient solution saltiness is 5 g/L in the culturing process, and the rising gradient is 5～10 g/L; The initial salt concn of ammonia nitrogen is 30～60mg/L, and the rising gradient of ammonia nitrogen concentration is 80～100 mg/L.
As preferred version, the treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, described nutrient solution prepares by following method: get acid hydrolysis casein 2.5g, yeast extract paste 8g, peptone 5.3g, trisodium citrate 6g, KCl 5g, MgSO
47H
2O 15.5g, calcium chloride 0.4g, ferric sulfate 0.015g, NaCl60.5g are dissolved in the 1000ml tap water, adjust the pH value and be 7 to 8 namely.The present invention is by a large amount of experiment screening enrichment culture liquid, and the required trace element of high efficiency nitrification bacterium gives by preparing described substratum, and nitrifier reproduction speed in this nutrient solution is fast.
NaCl and KCl that the salinity of nutrient solution adopts concentration to be respectively 15g/L and 5g/L simulate K
+Can be used as antagonist, reduce Na
+Murder by poisoning to microorganism.
In traditional empirical formula nitrobacteria nutrient solution, often add CaCO
3, its effect has: the one, for nitrobacteria provides carbon source; The 2nd, with acid-respons in the nutrient solution, can play the pH shock absorption, but CaCO
3Slightly soluble easily is deposited on reactor bottom, can't discharge with changing water, influences the growth of nitrobacteria.The present invention adds NaHCO through a large amount of experiment screenings in nutrient solution
3Substitute CaCO
3, can solve CaCO
3The problem brought of deposition, the buffer reagent of described nutrient solution are that concentration is that boric acid and the concentration of 0.15～0.2mg/L is the sodium bicarbonate of 40～60mg/L; Other ion in they and the solution together constitutes the buffered soln of nutrient solution, makes pH maintain 7 to 8, as preferably maintaining in 7.5～7.8 scopes.
As preferred version, the treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, the enrichment culture method of nitrobacteria are for to place solution on the shaking table, and 110 rev/mins of shaking speed, culture temperature are 20 to 30 ℃, and incubation time is 48 hours.
As preferred version, the treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, domestication time fs is about 4 days in first acclimation period, and the subordinate phase domestication time is about 6 days, from second acclimation period, each stage nitrobacteria domestication time lengthening.
As preferred version, the treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, in subordinate phase, look zoogleic formation situation, COD in the described enrichment culture liquid can obtain by adding methyl alcohol or glucose, concrete add-on can determine that according to the COD of processing waste water make COD concentration between 50mg/L～150mg/L, the input of ammonia nitrogen of the present invention is with NH
4The Cl form adds.
Because nitrobacteria is light, easily bubble through the water column, with drainage loss, therefore, in culturing process, add small amount of activated, can promote zoogleic formation.
High salinity provided by the invention, process for treating high ammonia nitrogen waste water, adopt batch activated sludge method (the experiment contrast shows: the batch technology salt resistance ability is better than Continuous Flow), with active sludge enrichment culture nitrobacteria mud, the enrichment culture of nitrobacteria is taked the mode that hockets, and namely a culture cycle comprises two stages: the cultivation of salt tolerant nitrobacteria and enrichment; Screening and the enrichment of the high efficiency nitrification bacterium of ammonia nitrogen in high density.
The present invention is directed to the characteristics of high salinity, high ammonia-nitrogen wastewater, the method of taking alternately to improve nutrient solution salinity or ammonia nitrogen concentration is carried out the enrichment of nitrifying sludge, through simulating salinity with NaCl and KCl, add various trace elements, do not adding or adding less under the condition of carbon source, through 48 days cultivation, filter out qualified nitrobacteria, and make nitrobacteria become dominant population.Final flora can tolerate salinity 35g/L, ammonia nitrogen concentration reaches the waste water of 500mg/L, and the water outlet ammonia nitrogen concentration is reduced to below the 10mg/L.By the high efficiency nitrification bacterium that this inventive method obtains, can satisfy the processing requirements of most of industrial high salinity, high ammonia-nitrogen wastewater.
Beneficial effect: high salinity provided by the invention; the treatment process of high-concentration ammonia nitrogenous wastewater is compared with prior art and had the following advantages: the present invention selects the nitrifier substratum according to a large amount of testing sieves and forms; the present invention is directed to high salinity; the characteristics of high ammonia-nitrogen wastewater; adopt batch activated sludge method; carry out the enrichment of nitrifying sludge by the method that alternately improves nutrient solution salinity or ammonia nitrogen concentration; obtain the nitrobacteria of anti-high salt and Nai Gao ammonia nitrogen through domestication; experimental result shows; the high efficiency nitrification bacterium of domestication can be handled 500 mg/L concentration ammonia nitrogens; saltiness 35 g/L are with interior high salinity waste water; the water outlet ammonia nitrogen concentration is reached in 10 mg/L; realize country-level qualified discharge; to handling the contour salt of tannery; high ammonia-nitrogen wastewater has good effect; can overcome nitrifier in the prior art and can not handle the shortcoming of the waste water of anti-high salt and Nai Gao ammonia nitrogen, environment protection is had very important significance.
Embodiment
Below in conjunction with specific embodiment, further illustrate the present invention, should understand these embodiment only is used for explanation the present invention and is not used in and limits the scope of the invention, after having read the present invention, those skilled in the art all fall within the application's claims institute restricted portion to the modification of the various equivalent form of values of the present invention.
Embodiment 1
The treatment process of a kind of high salinity, high-concentration ammonia nitrogenous wastewater may further comprise the steps:
Adopt batch activated sludge method, carry out enrichment by the method that improves nutrient solution ammonia nitrogen and salinity step by step, adopt NaCl, KCl simulation high salinity, initial salt concn is 5 g/L, final salt concn is 30～35 g/L, the ammonia nitrogen starting point concentration is 30～60mg/L, and ultimate density is 400～500mg/L, and COD concentration is 50mg/L～150mg/L; The enrichment culture of nitrobacteria is taked the mode that hockets, an enrichment culture cycle comprises two stages: the fs: the cultivation of salt tolerant nitrobacteria and enrichment, culture condition is: saltiness, with NaCl, KCl counts 5g/L, ammonia nitrogen concentration is 30～60mg/L, activated sludge concentration is 20～25g/L, temperature is 25 ~ 30 ℃, pH is that 7～8, DO is 4～6mg/L, and the fs does not add any additional carbon, domestication time fs is 4 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, enter subordinate phase: screening and the enrichment of the high efficiency nitrification bacterium of ammonia nitrogen in high density; The culture condition of subordinate phase is: saltiness, with NaCl, KCl counts 5g/L, ammonia nitrogen concentration 100～150mg/L, activated sludge concentration is 20～25g/L, 25 to 30 ℃ of temperature, and pH is 7～8, DO is 4～6mg/L, in subordinate phase, look zoogloea and form situation, add an amount of methyl alcohol, make COD concentration between 50mg/L～150mg/L, stimulate the heterotrophic bacterium growth with this, promote zoogloea to form, the subordinate phase domestication time is 6 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, finish subordinate phase, two stages have constituted an acclimation period; Enter the fs of second acclimation period then: the cultivation of salt tolerant nitrobacteria and enrichment, culture condition is: saltiness, with NaCl, KCl counts 15g/L, ammonia nitrogen concentration is 100～150mg/L, activated sludge concentration is 20～25g/L, temperature is 25 ~ 30 ℃, pH is 7～8, DO is 4～6mg/L, fs does not add any additional carbon, domestication time fs is 5 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, enter the subordinate phase of second acclimation period: culture condition is: saltiness, with NaCl, KCl counts 15g/L, ammonia nitrogen concentration 180～230mg/L, activated sludge concentration is 20～25g/L, 25 to 30 ℃ of temperature, pH is that 7～8, DO is 4～6mg/L, in subordinate phase, look zoogloea and form situation, add an amount of methyl alcohol, make COD concentration between 50mg/L～150mg/L, stimulate the heterotrophic bacterium growth with this, promote zoogloea to form, the subordinate phase domestication time is 7 days, goes out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, finish second acclimation period; Get second acclimation period then and screen the fs that the nitrifier that obtains carries out the 3rd acclimation period: the cultivation of salt tolerant nitrobacteria and enrichment, saltiness in the substratum, with NaCl, KCl counts 25g/L, ammonia nitrogen concentration is 180～230mg/L, activated sludge concentration is 20～25g/L, temperature is 25 ~ 30 ℃, pH is 7～8, DO is 4～6mg/L, fs does not add any additional carbon, domestication time fs is 5 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, enter the subordinate phase of the 3rd acclimation period: saltiness in the substratum, with NaCl, KCl counts 25g/L, ammonia nitrogen concentration 280～330mg/L, activated sludge concentration is 20～25g/L, 25 to 30 ℃ of temperature, pH is that 7～8, DO is 4～6mg/L, in subordinate phase, look zoogloea and form situation, add an amount of methyl alcohol, make COD concentration between 50mg/L～150mg/L, stimulate the heterotrophic bacterium growth with this, promote zoogloea to form, the subordinate phase domestication time is 8 days, goes out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, finish the 3rd acclimation period; Get the 3rd nitrifier behind the acclimation period then and carry out fs of the 4th acclimation period: the cultivation of salt tolerant nitrobacteria and enrichment, saltiness in the substratum, with NaCl, KCl counts 35g/L, ammonia nitrogen concentration is 280～330mg/L, activated sludge concentration is 20～25g/L, temperature is 25 ~ 30 ℃, pH is 7～8, DO is 4～6mg/L, fs does not add any additional carbon, domestication time fs is 6 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, enter the subordinate phase of the 4th acclimation period: saltiness in the substratum, with NaCl, KCl counts 35g/L, ammonia nitrogen concentration 380～500mg/L, activated sludge concentration is 20～25g/L, 25 to 30 ℃ of temperature, pH is that 7～8, DO is 4～6mg/L, in subordinate phase, look zoogloea and form situation, add an amount of methyl alcohol, make COD concentration between 50mg/L～150mg/L, stimulate the heterotrophic bacterium growth with this, promote zoogloea to form, the subordinate phase domestication time is 8 days, goes out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, finish the 4th acclimation period.Improve the concentration of nutrient solution ammonia nitrogen and salinity step by step by the above-mentioned method that hockets, the enrichment of nitrobacteria is divided into 4 acclimation periods, finally obtain handling ammonia nitrogen concentration 500 mg/L, saltiness 35 g/L with interior high salinity, high ammonia-nitrogen wastewater, the water outlet ammonia nitrogen concentration can reach 10 mg/L with interior anti-high salinity, the nitrobacteria of ammonia nitrogen in high density.
The treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, the mode that the enrichment culture of nitrobacteria is taked to hocket improves the concentration of nutrient solution ammonia nitrogen and salinity step by step, be total to 4 acclimation periods of enrichment culture, the rising gradient of nutrient solution saltiness is 5～10 g/L in the culturing process, and the rising gradient of ammonia nitrogen concentration is 80～100 mg/L.
The treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, described nutrient solution prepares by following method: get acid hydrolysis casein 2.5g, yeast extract paste 8g, peptone 5.3g, trisodium citrate 6g, KCl 5g, MgSO
47H
2O 15.5g, calcium chloride 0.4g, ferric sulfate 0.015g, NaCl60.5g are dissolved in the 1000ml tap water, and buffer reagent is that concentration is that boric acid and the concentration of 0.15～0.2mg/L is the sodium bicarbonate of 40～60mg/L.Adjust the pH value and be 7 to 8 namely.
The treatment process of above-described high salinity, high-concentration ammonia nitrogenous wastewater, the enrichment culture method of nitrobacteria are for to place solution on the shaking table, and 110 rev/mins of shaking speed, culture temperature are 20 to 30 ℃, and incubation time is 48 hours.
Embodiment 2 high salinity, high-concentration ammonia nitrogenous wastewater are handled experiment
Get high salt, the high ammonia-nitrogen wastewater of oil factory, chemical plant, fertilizer plant and tannery's discharging, the salt tolerant, the high efficiency nitrification of anti-ammonia nitrogen fungi pollution that adopt the domestication of the embodiment of the invention 1 method to obtain are inoculated into by 10% volume ratio and handled in each sewage 2 days, and concrete experimental result is as shown in table 1:
Table 1 high salinity, high-concentration ammonia nitrogenous wastewater result
Experimental result by table 1 shows, the present invention adopts batch activated sludge method, carry out the enrichment of nitrifying sludge by the method that alternately improves nutrient solution salinity or ammonia nitrogen concentration, can efficiently handle high salt, the high ammonia nitrogen that oil factory, chemical plant, fertilizer plant and tannery etc. discharge through the nitrobacteria that four cycle domestications obtain anti-high salt and Nai Gao ammonia nitrogen, the waste water of high COD value, the ammonia nitrogen of the waste water after the processing is less than 10mg/L, reach national sewage first discharge standard, have important economic benefits and social effect.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. the treatment process of a high salinity, high-concentration ammonia nitrogenous wastewater is characterized in that, may further comprise the steps:
Adopt batch activated sludge method, carry out enrichment by the method that improves nutrient solution ammonia nitrogen and salinity step by step, adopt NaCl, KCl simulation high salinity, initial salt concn is 5 g/L, final salt concn is 30～35 g/L, the ammonia nitrogen starting point concentration is 30～60mg/L, and ultimate density is 400～500mg/L, and COD concentration is 50mg/L～150mg/L; The enrichment culture of nitrobacteria is taked the mode that hockets, an enrichment culture cycle comprises two stages: the fs: the cultivation of salt tolerant nitrobacteria and enrichment, culture condition is: saltiness, with NaCl, KCl counts 5g/L, ammonia nitrogen concentration is 30～60mg/L, activated sludge concentration is 20～25g/L, temperature is 25 ~ 30 ℃, pH is that 7～8, DO is 4～6mg/L, and the fs does not add any additional carbon, domestication time fs is 2 to 4 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, enter subordinate phase: screening and the enrichment of the high efficiency nitrification bacterium of ammonia nitrogen in high density; The culture condition of subordinate phase is: saltiness, with NaCl, KCl counts 5g/L, ammonia nitrogen concentration 100～150mg/L, activated sludge concentration is 20～25g/L, 25 to 30 ℃ of temperature, and pH is 7～8, DO is 4～6mg/L, in subordinate phase, look zoogloea and form situation, add an amount of organic carbon, make COD concentration between 50mg/L～150mg/L, stimulate the heterotrophic bacterium growth with this, promote zoogloea to form, the subordinate phase domestication time is 4 to 6 days, go out water concentration as index with ammonia nitrogen, when ammonia nitrogen goes out water concentration less than 10mg/L, finish subordinate phase, two stages have constituted an acclimation period; Improve the concentration of nutrient solution ammonia nitrogen and salinity step by step by the above-mentioned method that hockets, the enrichment of nitrobacteria is divided into 4 acclimation periods, finally obtain handling ammonia nitrogen concentration 500 mg/L, saltiness 35 g/L with interior high salinity, high ammonia-nitrogen wastewater, the water outlet ammonia nitrogen concentration can reach 10 mg/L with interior anti-high salinity, the nitrobacteria of ammonia nitrogen in high density.
2. the treatment process of high salinity according to claim 1, high-concentration ammonia nitrogenous wastewater, it is characterized in that, the mode that the enrichment culture of nitrobacteria is taked to hocket improves the concentration of nutrient solution ammonia nitrogen and salinity step by step, be total to 4 acclimation periods of enrichment culture, the rising gradient of nutrient solution saltiness is 5～10 g/L in the culturing process, and the rising gradient of ammonia nitrogen concentration is 80～100 mg/L.
3. the treatment process of high salinity according to claim 1, high-concentration ammonia nitrogenous wastewater is characterized in that, described nutrient solution prepares by following method: get acid hydrolysis casein 2.5g, yeast extract paste 8g, peptone 5.3g, trisodium citrate 6g, KCl 5g, MgSO
47H
2O 15.5g, calcium chloride 0.4g, ferric sulfate 0.015g, NaCl60.5g are dissolved in the 1000ml tap water, adjust the pH value and be 7 to 8 namely.
4. the treatment process of high salinity according to claim 3, high-concentration ammonia nitrogenous wastewater is characterized in that, the buffer reagent of described nutrient solution is that concentration is that boric acid and the concentration of 0.15～0.2mg/L is the sodium bicarbonate of 40～60mg/L.
5. the treatment process of high salinity according to claim 1, high-concentration ammonia nitrogenous wastewater, it is characterized in that domestication time fs is 4 days in first acclimation period, the subordinate phase domestication time is 6 days, from second acclimation period, each stage nitrobacteria domestication time lengthening.
6. according to the treatment process of each described high salinity of claim 1 to 5, high-concentration ammonia nitrogenous wastewater, it is characterized in that, in subordinate phase, look zoogleic formation situation, add methyl alcohol or glucose, make COD concentration between 50mg/L～150mg/L.
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