CN111233166A - Method for biofilm formation of biofilm reactor by using microbial inoculum - Google Patents
Method for biofilm formation of biofilm reactor by using microbial inoculum Download PDFInfo
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Abstract
A method for biofilm formation by using a microbial inoculum, comprising the following steps: 1) a film formation preparation stage: 1-1) preparing nutrient solution with the same components as the wastewater to be treated; 1-2) adding a nutrient solution serving as a culture medium into a biological reaction tank of a wastewater treatment system, and adding a heterotrophic nitrification-aerobic denitrification composite microbial inoculum with the inoculum size of 5-10% to form a bacterial solution; 2) and (3) viable bacteria adsorption stage: operating a rotary biological membrane reactor in the biological reaction tank to enable living bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum to be adsorbed on the rotary biological membrane reactor to quickly form a light yellow biological membrane; 3) and (3) viable bacteria enrichment stage: detecting the value of OD600 and NH of the bacterial liquid in the biological reaction tank4 +The concentration of N, COD and TN to complete the enrichment of live bacteria in the bacterial liquid; 4) and (3) film formation finishing stage: and continuously adding the nutrient solution, and stopping adding the nutrient solution when a layer of tawny biomembrane is formed and protozoa is observed on the surface of the biomembrane and the biofilm formation of the rotary biomembrane reactor is finished.
Description
Technical Field
The invention relates to the field of wastewater treatment, in particular to a method for biofilm formation by using a microbial inoculum.
Background
The method is characterized in that the method is used for treating various high-organic-load, low-temperature, high-salt and high-ammonia-nitrogen wastewater from chemical manufacturing, printing and dyeing industry, livestock and poultry breeding, pharmaceutical sewage and landfill leachate, the wastewater has the advantages of high organic load, low temperature, high salt content and high ammonia nitrogen content, component redyeing, high biotoxicity, high pollutant concentration, large discharge amount and high treatment difficulty, and after the conventional wastewater treatment technology is adopted, all indexes of the wastewater still cannot reach the discharge standard and are directly discharged into a water source of a natural water body circulation system, so that C/N imbalance of the water source is caused, sufficient carbon sources cannot be provided for microorganisms, the growth of the microorganisms is inhibited, the quality of the water source is seriously influenced, the ecology and.
At present, biological methods are generally adopted for treating the wastewater, the biological methods comprise an activated sludge method and a biofilm method, microorganisms in the activated sludge method have poor tolerance, biomass and low treatment efficiency in extreme environments, so that the activated sludge method is less applied to highly difficult wastewater treatment, and the biofilm method has certain impact load resistance and tolerance performance and is more generally applied. However, at present, activated sludge is generally adopted as inoculated microorganisms in the biofilm method, and the biofilm culturing speed is slow, the period is long, the sludge yield is high, the amount of floating sludge is high, the tolerance is poor, and the like, so that the denitrification effect of the biofilm process is poor, and the operation cost and the operation and maintenance management difficulty are increased. Therefore, a simple and efficient biofilm formation starting method for the biomembrane process is sought, all indexes of the wastewater can reach the discharge standard after treatment, and the method becomes a key point for solving wastewater pollution.
Disclosure of Invention
The invention aims to provide a method for biofilm formation by using a microbial inoculum for a biofilm reactor, which improves a wastewater treatment process under the condition of not changing a wastewater treatment process and facilities, uses a heterotrophic nitrification-aerobic denitrification composite microbial inoculum, namely HN-AD microbial inoculum, in a wastewater treatment system, enriches and strengthens functional microorganisms in a rotary biofilm reactor by adjusting system operation parameters, and finally completes enrichment of dominant floras in the rotary biofilm reactor quickly and efficiently, thereby improving the wastewater treatment efficiency of the whole wastewater treatment system.
The purpose of the invention is realized by adopting the following scheme:
a method for biofilm formation by using a microbial inoculum, comprising the following steps:
1) a film formation preparation stage:
1-1) preparing nutrient solution with the same components as the wastewater to be treated;
1-2) adding the nutrient solution prepared in the step 1-1) into a biological reaction tank of a wastewater treatment system to serve as a culture medium, and adding a heterotrophic nitrification-aerobic denitrification composite microbial inoculum with the inoculum size of 5-10% according to the volume of the nutrient solution to form a bacterial solution;
2) and (3) viable bacteria adsorption stage:
operating a rotary biological membrane reactor in a biological reaction tank of a wastewater treatment system, and controlling the linear velocity of the edge of the rotary biological membrane reactor to be 1-5 m/min, so that living bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum are adsorbed on the rotary biological membrane reactor to quickly form a light yellow biological membrane;
3) and (3) viable bacteria enrichment stage:
detecting the value of OD600 and NH of the bacterial liquid in the biological reaction tank4 +Concentration of N, COD, TN if the value of OD600 is less than 1, and NH4 +The concentrations of-N, COD and TN are respectively lower than 6.5mg/L, 400mg/L and 108mg/L,continuously adding the nutrient solution into the biological reaction tank to ensure that the OD600 value of the bacterial solution reaches 1-1.5 and the viable bacteria content in the bacterial solution reaches 180-200 hundred million/g, namely finishing the enrichment of the viable bacteria in the bacterial solution;
4) and (3) film formation finishing stage:
after finishing the enrichment of the live bacteria in the bacteria liquid, detecting NH of the bacteria liquid in the biological reaction tank4 +Concentration of N, COD, TN, once NH4 +The concentration of N, COD and TN is lower than 6.5mg/L, 400mg/L and 108mg/L, nutrient solution is continuously added into the biological reaction tank until a layer of tawny biological membrane is formed on the rotary biological membrane reactor, protozoa are observed on the surface of the biological membrane through a microscope, and the value of OD600 and NH of bacteria liquid in the biological reaction tank are detected4 +Concentration of N, COD, TN, NH when OD600 of the bacterial liquid is less than 0.354 +And (4) respectively reducing the concentrations of-N, COD and TN to be lower than 8.8 mg/L, 280 mg/L and 81mg/L, namely completing biofilm formation of the rotary biofilm reactor, and stopping adding the nutrient solution.
When the wastewater to be treated in the step 1-1) is high ammonia nitrogen wastewater, the nutrient solution comprises the following components: 3.1g/L of anhydrous sodium acetate, 2.2g/L of ammonium sulfate, 0.7 g/L of dipotassium hydrogen phosphate, 2.0g/L of magnesium sulfate heptahydrate, 0.1g/L of magnesium sulfate monohydrate, 1.5g/L of calcium chloride and 0.1g/L of ferrous sulfate heptahydrate.
The preparation steps of the nutrient solution are as follows:
1) preparing solution of trace elements
The trace element solution comprises 2.0g/L of mixed magnesium sulfate heptahydrate, 0.1g/L of magnesium sulfate monohydrate, 1.5g/L of calcium chloride and 0.1g/L of ferrous sulfate heptahydrate;
2) preparing simulated wastewater
Calculating the adding amount of the carbon source, the nitrogen source and the phosphorus source by using the effective volume of the biological reaction tank, wherein the adding proportion is as follows: 3.1g/L of anhydrous sodium acetate, 2.2g/L of ammonium sulfate and 0.7 g/L of dipotassium hydrogen phosphate;
3) preparing nutrient solution
Simulated wastewater prepared by the following steps: microelement solution =1L:5mL, mixed to form.
The heterotrophic nitrification-aerobic denitrification composite microbial inoculum comprises cuppridinium bulimia SWA1, alcaligenes faecalis, acinetobacter and ochrobactrum TAC-2.
The content ratio of the cuppridinium bulimia SWA1, the alcaligenes faecalis, the acinetobacter and the ochrobactrum anthropi TAC-2 in the heterotrophic nitrification-aerobic denitrification composite microbial agent is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
And the temperature of the bacterial liquid in the biological reaction tank is kept at 25-30 ℃.
The rotary biofilm reactor is a biological rotating disc or a biological rotating cage.
The filler in the rotary biomembrane reactor is a three-dimensional structure biological disc.
The three-dimensional structure biological disc comprises a biological disc with a concave-convex plate structure, a biological disc with a corrugated plate structure, a biological disc with a reticular plate structure, a biological disc with a convex surface structure and a three-dimensional structure formed by combining the structures.
The invention has the following beneficial effects:
(1) the invention can remove most pollutants in the wastewater, reduce the operation load of the system, obviously improve the process treatment effect, and improve the removal rate of COD by 20-40% and the removal rate of ammonia nitrogen and total nitrogen by 20-50%;
(2) the concentration of microorganisms in the rotary type biofilm reactor can reach 5000-8000 mg/L, the biofilm formation speed is high, the enrichment of heterotrophic nitrification-aerobic denitrification composite bacteria in a biological reaction tank is facilitated, the impact load resistance is high, and the biochemical efficiency is high;
(3) compared with the traditional activated sludge system or the anaerobic-aerobic combined wastewater treatment system, the method can greatly shorten the flow, save the process capital construction cost, realize the high-efficiency and stable removal of pollutants in the wastewater only by a two-stage or multi-stage full-aerobic system, and save the land use area by 50 percent;
(4) compared with the traditional wastewater treatment process, the invention adopts a natural contact reoxygenation mode to maintain the full aerobic environment in the rotary type biomembrane reactor, can simultaneously realize the synchronous removal of ammonia nitrogen, nitrate nitrogen and total nitrogen in one reactor, and can simultaneously remove organic matters, the process flow is short, the modular design is realized, the structure is compact, the construction and operation cost is reduced by 30%, and the operation and maintenance management difficulty is lower;
(5) the invention is suitable for high organic load, low temperature, high salt and high ammonia nitrogen wastewater, and can be applied to low-carbon domestic wastewater, chemical wastewater and landfill leachate, no excess sludge is generated, the bacterial sludge discharge amount is less, and the operation cost is low;
(6) the operation management and maintenance of the biological system adopting the microbial inoculum to form the membrane are simple, the field maintenance of professional personnel is not needed, the regional automatic control management is easy to realize, and the operation cost is low.
The invention has the advantages that because the culture medium for domesticating the heterotrophic nitrification-aerobic denitrification composite bacteria is prepared according to the components of the wastewater to be treated, when the actual wastewater treatment is carried out, the domesticated heterotrophic nitrification-aerobic denitrification composite bacteria do not need to spend a large amount of time for re-domesticating wastewater with different components, so the membrane hanging speed is high, the high ammonia nitrogen tolerance performance is good, the invention is not limited to a wastewater treatment system of which the biofilm reactor is a rotary biofilm reactor, and the invention can also be suitable for various aerobic biofilm reactors, and realizes the high-efficiency and quick membrane hanging starting of the aerobic system.
Drawings
FIG. 1 is a flow chart of the present invention;
FIG. 2 shows NH in the conventional sludge biofilm formation process in example 14 +-N index change profiles;
FIG. 3 shows NH of the present invention in example 14 +-N index change profiles;
FIG. 4 is a schematic diagram showing the change of each water quality index in the present invention in example 2.
Detailed Description
As shown in fig. 1 to 4, a method for biofilm formation using a microbial agent includes the steps of:
1) a film formation preparation stage:
1-1) preparing a nutrient solution which has the same components as the wastewater to be treated, wherein the purity specification of each component of the nutrient solution is analytically pure;
when the wastewater to be treated in the step 1-1) is high ammonia nitrogen wastewater, the nutrient solution comprises the following components: 3.1g/L of anhydrous sodium acetate, 2.2g/L of ammonium sulfate, 0.7 g/L of dipotassium hydrogen phosphate, 2.0g/L of magnesium sulfate heptahydrate, 0.1g/L of magnesium sulfate monohydrate, 1.5g/L of calcium chloride and 0.1g/L of ferrous sulfate heptahydrate.
The preparation steps of the nutrient solution are as follows:
1) preparing solution of trace elements
The trace element solution comprises 2.0g/L of mixed magnesium sulfate heptahydrate, 0.1g/L of magnesium sulfate monohydrate, 1.5g/L of calcium chloride and 0.1g/L of ferrous sulfate heptahydrate;
2) preparing simulated wastewater
Calculating the adding amount of the carbon source, the nitrogen source and the phosphorus source by using the effective volume of the biological reaction tank, wherein the adding proportion is as follows: 3.1g/L of anhydrous sodium acetate, 2.2g/L of ammonium sulfate and 0.7 g/L of dipotassium hydrogen phosphate;
3) preparing nutrient solution
Simulated wastewater prepared by the following steps: microelement solution =1L:5mL, mixed to form.
1-2) adding the nutrient solution prepared in the step 1-1) into a biological reaction tank of a wastewater treatment system to serve as a culture medium, and adding a heterotrophic nitrification-aerobic denitrification composite microbial inoculum with the inoculum size of 5-10% according to the volume of the nutrient solution to form a bacterial solution;
and the temperature of the bacterial liquid in the biological reaction tank is kept at 25-30 ℃.
The heterotrophic nitrification-aerobic denitrification composite microbial inoculum comprises cuppridinium bulimia SWA1, alcaligenes faecalis, acinetobacter and ochrobactrum TAC-2. The content ratio of the cuppridinium bulimia SWA1, the alcaligenes faecalis, the acinetobacter and the ochrobactrum anthropi TAC-2 in the heterotrophic nitrification-aerobic denitrification composite microbial agent is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
2) And (3) viable bacteria adsorption stage:
operating a rotary biological membrane reactor in a biological reaction tank of a wastewater treatment system, and controlling the linear velocity of the edge of the rotary biological membrane reactor to be 1-5 m/min, so that living bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum are adsorbed on the rotary biological membrane reactor to quickly form a light yellow biological membrane;
the rotary biofilm reactor is a biological rotating disc or a biological rotating cage. The filler in the rotary biomembrane reactor is a three-dimensional structure biological disc. The three-dimensional structure biological disc comprises a biological disc with a concave-convex plate structure, a biological disc with a corrugated plate structure, a biological disc with a reticular plate structure, a biological disc with a convex surface structure and a three-dimensional structure formed by combining the structures.
3) And (3) viable bacteria enrichment stage:
detecting the value of OD600 and NH of the bacterial liquid in the biological reaction tank4 +Concentration of N, COD, TN if the value of OD600 is less than 1, and NH4 +When the concentration of N, COD and TN is respectively lower than 6.5mg/L, 400mg/L and 108mg/L, continuously adding nutrient solution into the biological reaction tank to ensure that the OD600 value of the bacterial solution reaches 1-1.5 and the content of viable bacteria in the bacterial solution reaches 180-200 hundred million/g, thus finishing the enrichment of the viable bacteria in the bacterial solution;
4) and (3) film formation finishing stage:
after finishing the enrichment of the live bacteria in the bacteria liquid, detecting NH of the bacteria liquid in the biological reaction tank4 +Concentration of N, COD, TN, once NH4 +The concentration of N, COD and TN is lower than 6.5mg/L, 400mg/L and 108mg/L, nutrient solution is continuously added into the biological reaction tank until a layer of tawny biological membrane is formed on the rotary biological membrane reactor, protozoa are observed on the surface of the biological membrane through a microscope, and the value of OD600 and NH of bacteria liquid in the biological reaction tank are detected4 +Concentration of N, COD, TN, NH when OD600 of the bacterial liquid is less than 0.354 +And (4) respectively reducing the concentrations of-N, COD and TN to be lower than 8.8 mg/L, 280 mg/L and 81mg/L, namely completing biofilm formation of the rotary biofilm reactor, and stopping adding the nutrient solution.
The invention will now be further illustrated by reference to the following examples:
example 1:
after solid-liquid separation of the dung residues and the wastewater is realized by a solid-liquid separator for raw water of the breeding wastewater of a certain pig farm, nutrient solution is prepared according to components of obtained supernatant, and the effective volume of the nutrient solution added into a wastewater treatment system is 100m3After the biological reaction tank is used, keeping the temperature of a solution in the biological reaction tank at 25-28 ℃, adding 5-10% of heterotrophic nitrification-aerobic denitrification composite microbial inoculum formed by compounding 10-20% of cupropriosis SwA1, 5-20% of alcaligenes faecalis, 10-30% of acinetobacter and 20-50% of ochrobactrum occulta TAC-2 into the biological reaction tank according to the volume of a nutrient solution to form a bacterial solution, operating a rotary biological membrane reactor in the biological reaction tank of a wastewater treatment system, and controlling the linear velocity of the edge of the rotary biological membrane reactor at 2.5m/min to enable the viable bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum to be adsorbed on the rotary biological membrane reactor to quickly form a light yellow biological membrane;
detecting the value of OD600 and NH of the bacterial liquid in the biological reaction tank every 3d4 +Concentration of N, COD, TN if the value of OD600 is less than 1, and NH4 +When the concentration of N, COD and TN is respectively lower than 6.5mg/L, 400mg/L and 108mg/L, adding a nutrient solution into the biological reaction tank to ensure that the OD600 value of the bacterial liquid reaches 1-1.5 and the content of viable bacteria in the bacterial liquid reaches 180-200 hundred million/g, thus finishing the enrichment of the viable bacteria in the bacterial liquid;
after finishing the enrichment of live bacteria in the bacteria liquid, detecting NH of the bacteria liquid in the biological reaction tank every 3d4 +Concentration of N, COD, TN, once NH4 +The concentration of N, COD and TN is lower than 6.5mg/L, 400mg/L and 108mg/L, nutrient solution is continuously added into the biological reaction tank until a layer of tawny biological membrane is formed on the rotary biological membrane reactor, protozoa are observed on the surface of the biological membrane through a microscope, and the value of OD600 and NH of bacteria liquid in the biological reaction tank are detected4 +Concentration of N, COD, TN, at which OD600 of the bacterial liquid is less than 0.35, NH4 +The concentrations of-N, COD and TN are respectively lower than 8.8 mg/L, 280 mg/L and 81mg/L, namely the biofilm formation of the rotary biofilm reactor is finished, the nutrient solution is stopped to be added, and the wastewater is treatedAnd adding a supernatant obtained by solid-liquid separation into a biological reaction tank of the treatment system for wastewater treatment, wherein the hydraulic retention time is 20-30 h.
The wastewater treatment system is a wastewater treatment system for treating 50 tons of piggery wastewater every day, and NH is formed after film formation is successfully carried out to stably run in one month before and after the application of the system4 +the-N removal efficiency is shown in figure 1 and figure 2, and the comparison between before and after the use of the invention can find that the sludge biofilm reactor without the invention has 33d of time for starting and poor denitrification effect, but the biofilm reactor of the invention has the advantages that the living bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum have higher tolerance, are easier to enrich than common microorganisms, have faster growth speed, have 15d of time for biofilm formation, have higher denitrification effect and NH4 +The N removal rate is improved by 13.2 percent, and all indexes of the treated wastewater meet the livestock and poultry breeding wastewater discharge standard (GB 18596-2001).
Example 2:
preparing nutrient solution according to components of a wastewater stock solution of a certain cattle farm, and adding the nutrient solution into a wastewater treatment system, wherein the effective volume of the wastewater treatment system is 100m3After the biological reaction tank, keeping the temperature of the solution in the biological reaction tank at 20-30 ℃, adding 5-10% of heterotrophic nitrification-aerobic denitrification composite microbial inoculum formed by compounding 10-20% of copper-greedy bacteria SWA1, 5-20% of alcaligenes faecalis, 10-30% of acinetobacter and 20-50% of ochrobactrum anthropi TAC-2 into the biological reaction tank according to the volume of the nutrient solution to form a bacterial solution, operating the rotary biological membrane reactor in the biological reaction tank of the wastewater treatment system, controlling the linear speed of the edge of the rotary biological membrane reactor at 2.5m/min, enabling the viable bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum to be adsorbed on the rotary biological membrane reactor, and rapidly reducing the OD600 value of the bacterial solution at day 3 to ensure that NH 600 is rapidly reduced4 +Average removal rates of-N, COD and TN are respectively 98.3%, 83.8% and 81.4%, and a light yellow biological film is rapidly formed on the rotary biological film reactor;
NH of bacterial liquid after 3d4 +The concentrations of-N, COD and TN are respectively reduced to 30 mg/L, 250.8 mg/L and 108.3mg/L, and the removal rates can respectively reach 943%, 90.7% and 81.4%, and the OD600 value of the bacterial liquid is increased to 1.3, thus completing the enrichment of viable bacteria in the bacterial liquid and gradually increasing the thickness of the biological membrane on the disk.
After finishing the enrichment of live bacteria in the bacteria liquid, detecting NH of the bacteria liquid in the biological reaction tank every 3d4 +Concentration of N, COD, TN, once NH4 +The concentration of N, COD and TN is lower than 6.5mg/L, 400mg/L and 108mg/L, nutrient solution is continuously added into the biological reaction tank until a layer of tawny biological membrane is formed on the rotary biological membrane reactor, protozoa are observed on the surface of the biological membrane through a microscope, and the value of OD600 and NH of bacteria liquid in the biological reaction tank are detected4 +Concentration of-N, COD, TN, NH4 +And (3) respectively reducing the concentration of N, COD and TN to 6.6mg/L, 258.9mg/L and 73.1mg/L, keeping the concentration stable, and stopping adding the nutrient solution for 15-20 days when the removal rate of each pollutant is high, namely the biofilm formation of the rotary biofilm reactor is finished.
Adding a wastewater stock solution of a certain cattle farm into a biological reaction tank of a wastewater treatment system for wastewater treatment, wherein the hydraulic retention time is 20-30 h, and the OD600 value and NH of the treated wastewater4 +The concentrations of N, COD and TN are shown in the following figure 3, and all indexes meet the livestock and poultry breeding wastewater discharge standard (GB 18596-2001).
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and those skilled in the art can make modifications without departing from the spirit of the present invention.
Claims (9)
1. A method for biofilm formation of a biofilm reactor by using a microbial inoculum is characterized by comprising the following steps:
1) a film formation preparation stage:
1-1) preparing nutrient solution with the same components as the wastewater to be treated;
1-2) adding the nutrient solution prepared in the step 1-1) into a biological reaction tank of a wastewater treatment system to serve as a culture medium, and adding a heterotrophic nitrification-aerobic denitrification composite microbial inoculum with the inoculum size of 5-10% according to the volume of the nutrient solution to form a bacterial solution;
2) and (3) viable bacteria adsorption stage:
operating a rotary biological membrane reactor in a biological reaction tank of a wastewater treatment system, and controlling the linear velocity of the edge of the rotary biological membrane reactor to be 1-5 m/min, so that living bacteria of the heterotrophic nitrification-aerobic denitrification composite microbial inoculum are adsorbed on the rotary biological membrane reactor to quickly form a light yellow biological membrane;
3) and (3) viable bacteria enrichment stage:
detecting the value of OD600 and NH of the bacterial liquid in the biological reaction tank4 +Concentration of N, COD, TN if the value of OD600 is less than 1, and NH4 +When the concentration of N, COD and TN is respectively lower than 6.5mg/L, 400mg/L and 108mg/L, continuously adding nutrient solution into the biological reaction tank to ensure that the OD600 value of the bacterial solution reaches 1-1.5 and the content of viable bacteria in the bacterial solution reaches 180-200 hundred million/g, thus finishing the enrichment of the viable bacteria in the bacterial solution;
4) and (3) film formation finishing stage:
after finishing the enrichment of the live bacteria in the bacteria liquid, detecting NH of the bacteria liquid in the biological reaction tank4 +Concentration of N, COD, TN, once NH4 +The concentration of N, COD and TN is lower than 6.5mg/L, 400mg/L and 108mg/L, nutrient solution is continuously added into the biological reaction tank until a layer of tawny biological membrane is formed on the rotary biological membrane reactor, protozoa are observed on the surface of the biological membrane through a microscope, and the value of OD600 and NH of bacteria liquid in the biological reaction tank are detected4 +Concentration of N, COD, TN, NH when OD600 of the bacterial liquid is less than 0.354 +And (4) respectively reducing the concentrations of-N, COD and TN to be lower than 8.8 mg/L, 280 mg/L and 81mg/L, namely completing biofilm formation of the rotary biofilm reactor, and stopping adding the nutrient solution.
2. The method of biofilm reactor biofilm formation using microbial agents of claim 1, wherein: when the wastewater to be treated in the step 1-1) is high ammonia nitrogen wastewater, the nutrient solution comprises the following components: 3.1g/L of anhydrous sodium acetate, 2.2g/L of ammonium sulfate, 0.7 g/L of dipotassium hydrogen phosphate, 2.0g/L of magnesium sulfate heptahydrate, 0.1g/L of magnesium sulfate monohydrate, 1.5g/L of calcium chloride and 0.1g/L of ferrous sulfate heptahydrate.
3. A method of biofilm reactor biofilm formation using microbial agents as claimed in claim 2, wherein: the preparation steps of the nutrient solution are as follows:
1) preparing solution of trace elements
The trace element solution comprises 2.0g/L of mixed magnesium sulfate heptahydrate, 0.1g/L of magnesium sulfate monohydrate, 1.5g/L of calcium chloride and 0.1g/L of ferrous sulfate heptahydrate;
2) preparing simulated wastewater
Calculating the adding amount of the carbon source, the nitrogen source and the phosphorus source by using the effective volume of the biological reaction tank, wherein the adding proportion is as follows: 3.1g/L of anhydrous sodium acetate, 2.2g/L of ammonium sulfate and 0.7 g/L of dipotassium hydrogen phosphate;
3) preparing nutrient solution
Simulated wastewater prepared by the following steps: microelement solution =1L:5mL, mixed to form.
4. The method of biofilm reactor biofilm formation using microbial agents of claim 1, wherein: the heterotrophic nitrification-aerobic denitrification composite microbial inoculum comprises cuppridinium bulimia SWA1, alcaligenes faecalis, acinetobacter and ochrobactrum TAC-2.
5. The method of biofilm reactor biofilm formation using microbial agents of claim 4, wherein: the content ratio of the cuppridinium bulimia SWA1, the alcaligenes faecalis, the acinetobacter and the ochrobactrum anthropi TAC-2 in the heterotrophic nitrification-aerobic denitrification composite microbial agent is 10-20%: 5-20%: 10-30%: 20 to 50 percent.
6. The method of biofilm reactor biofilm formation using microbial agents of claim 1, wherein: and the temperature of the bacterial liquid in the biological reaction tank is kept at 25-30 ℃.
7. The method of biofilm reactor biofilm formation using microbial agents of claim 1, wherein: the rotary biofilm reactor is a biological rotating disc or a biological rotating cage.
8. The method of biofilm reactor biofilm formation using microbial agents of claim 1, wherein: the filler in the rotary biomembrane reactor is a three-dimensional structure biological disc.
9. A method of biofilm reactor biofilm formation using microbial agents as claimed in claim 8, wherein: the three-dimensional structure biological disc comprises a biological disc with a concave-convex plate structure, a biological disc with a corrugated plate structure, a biological disc with a reticular plate structure, a biological disc with a convex surface structure and a three-dimensional structure formed by combining the structures.
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