CN106542655B - Rapid biofilm formation method for efficient denitrification microbial agent - Google Patents
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Abstract
A high-efficiency denitrification microorganism fast film forming method comprises the steps of adding a biological filler into an aerobic nitrification reactor, and internally connecting activated sludge into the aerobic nitrification reactor; adding artificial simulated wastewater every 1-2 d, controlling the temperature, dissolved oxygen and pH of the reactor, allowing high-concentration microorganisms to enter the aerobic nitrification reactor from the top through an external circulation pipeline, and forming a solid-liquid flow from top to bottom in a biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate. The method can thoroughly overcome the defects of long time, poor adhesion, complex operation, low biomass and poor impact load resistance of the denitrification microorganisms for biofilm formation, improve the fixation amount of nitrobacteria, realize fast biofilm formation, and enhance the capacity of resisting the change of the external environment and the capacity of resisting impact load.
Description
Technical Field
The invention belongs to the technical field of water treatment, and relates to a rapid biofilm formation method of a high-efficiency denitrification microbial agent.
Background
In the existing ammonia nitrogen wastewater treatment technology, an activated sludge process is generally adopted, and has the advantages of high treatment capacity, high treatment efficiency and good effluent quality, various deformation processes have good nitrogen and phosphorus removal performance and are widely applied to urban sewage treatment, and the defects of difficulty in obtaining high biomass of nitrogen removal microorganisms and low nitrification efficiency in an activated sludge system, so that an autotrophic microorganism nitrogen removal system is weak in impact resistance, incomplete in nitrification, large in occupied area, high in capital construction investment, high in operation maintenance and management difficulty and the like are caused. In recent years, with the continuous improvement of the sewage biological treatment technology, the biofilm method is gradually applied to the biological denitrification technology, and is widely applied to the treatment of domestic sewage and industrial wastewater due to the advantages of stable operation, less residual sludge, simple management, strong removal capability on ammonia nitrogen and refractory pollutants, capability of adapting to larger water quality range change and the like.
The biomembrane method is characterized in that microorganisms (nitrifying bacteria and denitrifying bacteria) are added in the sewage treatment to be attached or fixed on the surface of a biological filler to form a layer of biomembrane, solid-liquid two-phase mass transfer is carried out through the contact of the wastewater and the biomembrane, and the biodegradation of organic matters is carried out through the biomembrane to realize the degradation of harmful components such as ammonia nitrogen and the like in the sewage. According to different operation modes, the biological filter, the biological rotating disc, the biological contact oxidation method and the like can be divided, and although the structures of the biological filter, the biological rotating disc and the biological contact oxidation method are greatly different, the basic principles are the same. The quality of the biological film is directly related to the treatment effect of the sewage treatment device, and the influencing factors comprise two aspects, namely a film hanging method and the surface performance of the biological filler. Therefore, the key point is that enough microorganisms are required to be available before film formation, and the microorganism can grow in the environment that wastewater enters a bioreactor, and once the microorganism is attached to the filler, the microorganism can rapidly proliferate; secondly, when the membrane is hung, the microorganism in the bacteria liquid has stronger adhesive force with the biological filler.
Although the biofilm method has higher treatment efficiency, the method has obvious effect on the water body slightly polluted by organic matters and ammonia nitrogen. However, the biofilm method of the microbial biofilm technology has many problems which are not solved. For example, the biofilm formation time is long, the biofilm is easy to fall off, the biomass is low, the ammonia nitrogen removal efficiency is high, and the initial investment for the filler and the supporting structure is high, so that the research on a rapid biofilm formation method has great significance for the biofilm sewage treatment process.
Disclosure of Invention
The invention provides a rapid biofilm formation method of a high-efficiency denitrification microbial agent, aiming at the defects of long time, poor adhesion, complex operation, low biomass and low impact load resistance of the existing biological denitrification microbial agent. The rapid biofilm formation method can thoroughly solve the problems, so that the growth condition of microorganisms on the biological filler is good, and the stable effect of the biological membrane on treating the wastewater containing ammonia nitrogen is ensured.
The technical problem to be solved by the present invention is achieved by the following technical means. The invention relates to a rapid biofilm formation method for efficient denitrification microorganisms, which is characterized in that the method adopts an aerobic nitrification reactor to form a biofilm, and the specific method comprises the following steps: adding a biological filler into an aerobic nitrification reactor, and internally connecting activated sludge into the aerobic nitrification reactor; adding artificial simulation wastewater every 1-2 d into the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 30-50% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 50-150 mg/L; high-concentration microorganisms with the concentration of suspended matters of bacteria liquid of 3000-5000 mg/L enter the aerobic nitrification reactor from the top through an external circulation pipeline, and solid-liquid flow from top to bottom is formed in a biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: the following method was used for actual wastewater operation: actual ammonia nitrogen-containing wastewater is continuously added into the aerobic nitrification reactor and runs for 7-14 days, the ammonia nitrogen concentration is 100-200 mg/L, and the ammonia nitrogen removal rate of effluent is continuously more than or equal to 95% for 7 days.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: the method comprises the following specific steps:
(1) filling biological fillers: adding a biological filler into an aerobic nitrification reactor, wherein the filling volume is 60-90% of the effective volume of the reactor;
(2) inoculating activated sludge: after activated sludge is inoculated into the aerobic nitrification reactor, stuffy aeration is carried out for 24-48 h, and the inoculum size accounts for 50-70% of the effective volume of the reactor after filling with the filler;
(3) adding high-concentration denitrifying microorganisms: adding high-concentration denitrifying microorganisms into an external circulation device, wherein the concentration of suspended matters in the bacteria liquid is 3000-5000 mg/L.
(4) Film forming of the aerobic nitrification reactor: adding artificial simulation wastewater every 1-2 d into the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 30-50% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 50-150 mg/L; high-concentration microorganisms enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in the biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate; during the period, the circulation device does not discharge mud, and no supernatant flows out;
(5) actual wastewater operation: continuously adding actual ammonia nitrogen-containing wastewater into the aerobic nitrification reactor for 7-14 days, wherein the ammonia nitrogen concentration is 100-200 mg/L, and finishing biofilm formation when the ammonia nitrogen removal rate of effluent is continuously more than or equal to 95% for 7 days; during the period, the circulation device begins to discharge mud, and the supernatant liquid continuously flows out.
The artificial simulated wastewater is artificially prepared by a conventional method according to the main components of actual wastewater.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: the biological fillers include but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: the activated sludge in the step (2) is taken from and not limited to denitrification sludge of an aerobic tank of a municipal sewage treatment plant.
The invention relates to high-efficiency denitrificationThe further preferable technical scheme of the method for quickly forming the membrane by the microorganisms is as follows: the high-concentration microorganism in the step (3) adopts Paracoccus aminovorans (A)Paracoccus aminovorans) LH-N40CGMCC No. 6971.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: the artificial simulated wastewater mainly comprises the following components: from (NH)4）2SO40.5-5 g/L, 0.5-1 g/L glucose, MgSO40.01～0.02g/L，K2HPO40.1～0.3g/L，FeSO4·7H20.05-0.1 g/L of O, 1L of distilled water and trace element liquid.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: the microelement liquid comprises the following main components: 50-150 mg/L CoCl2·6H2O、50~150mg/L CaCl2、10~50mg/L CuSO4·5H2O、50~150mg/L ZnSO4·7H2O、50~150mg/L FeSO4·7H2O、50~150mg/LMnSO4·2H2O。
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: in the film forming stage, the temperature is 10-35 ℃; the pH value is 6.0-10.0; DO is 0.5-4.0 mg/L.
The invention relates to a high-efficiency denitrification microorganism rapid biofilm culturing method, which further adopts the preferable technical scheme that: practical wastewaters include, but are not limited to, the following industrial wastewaters: domestic sewage, coal chemical industry, synthetic ammonia, printing and dyeing, breeding and medicine.
The invention has the beneficial effects that:
(1) the advantages of an activated sludge method and a biofilm method are combined, and the suspended filler is added as the filler for the attachment growth of microorganisms, so that the fixation amount of nitrobacteria is increased, and the capacity of resisting the change of the external environment and the capacity of resisting impact load are enhanced;
(2) the added high-concentration microorganisms are utilized to carry out external circulation, the concentration of the denitrification microorganisms in the reactor is increased, the biofilm formation of the denitrification microorganisms is strengthened, and the biofilm formation speed and the biofilm formation thickness of the suspended filler are improved.
(3) The actual wastewater is adopted for biofilm formation impact, and the stability of biofilm formation, impact load resistance and aeration and liquid shearing resistance are improved.
(4) The method is simple, easy to operate and has wide practicability.
Detailed Description
Embodiment 1, a method for rapid biofilm formation of efficient denitrification microorganisms, which adopts an aerobic nitrification reactor to carry out biofilm formation, and comprises the following steps: adding artificial simulation wastewater every 1d in the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 30% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 50 mg/L; high-concentration microorganisms with the concentration of suspended matters of bacteria liquid of 3000mg/L enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in a biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate.
Embodiment 2, a method for rapid biofilm formation of efficient denitrification microorganisms, which adopts an aerobic nitrification reactor to carry out biofilm formation, and comprises the following steps: adding artificial simulation wastewater every 2d in the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 50% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 150 mg/L; high-concentration microorganisms with the concentration of the suspended matters of the bacteria liquid of 5000mg/L enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in a biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate.
In the method for the efficient denitrification microorganism rapid biofilm formation described in the embodiment 3, the embodiment 1 or the embodiment 2, the following method is adopted to carry out actual wastewater operation: actual ammonia nitrogen-containing wastewater is continuously added into the aerobic nitrification reactor for 7 days, the ammonia nitrogen concentration is 100mg/L, and the ammonia nitrogen removal rate of effluent is continuously more than or equal to 95 percent for 7 days.
In the embodiment 4, the efficient denitrification microorganism rapid biofilm formation method described in the embodiment 1 or 2, the following method is adopted to carry out actual wastewater operation: actual ammonia nitrogen-containing wastewater is continuously added into the aerobic nitrification reactor for 14 days, the ammonia nitrogen concentration is 200mg/L, and the ammonia nitrogen removal rate of effluent is continuously 7 days or more than 95%.
Embodiment 5, a method for rapid biofilm formation of efficient denitrification microorganisms, the method comprises the following steps:
(1) filling biological fillers: adding a biological filler into an aerobic nitrification reactor, wherein the filling volume is 60 percent of the effective volume of the reactor;
(2) inoculating activated sludge: after activated sludge is inoculated into the aerobic nitrification reactor, stuffy aeration is carried out for 24 hours, and the inoculum size accounts for 50 percent of the effective volume of the reactor after filling;
(3) adding high-concentration denitrifying microorganisms: high-concentration denitrifying microorganisms are added into the external circulation device, and the concentration of the suspended matters in the bacteria liquid is 3000 mg/L.
(4) Film forming of the aerobic nitrification reactor: adding artificial simulation wastewater every 1d in the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 30% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 50 mg/L; high-concentration microorganisms enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in the biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate; during the period, the circulation device does not discharge mud, and no supernatant flows out;
(5) actual wastewater operation: continuously adding actual ammonia nitrogen-containing wastewater into the aerobic nitrification reactor for 7 days, wherein the ammonia nitrogen concentration is 100mg/L, and finishing biofilm formation when the removal rate of the ammonia nitrogen in the effluent is continuously more than or equal to 95% for 7 days; during the period, the circulation device begins to discharge mud, and the supernatant liquid continuously flows out.
The biological fillers include but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers.
The activated sludge in the step (2) is taken from and not limited to denitrification sludge of an aerobic tank of a municipal sewage treatment plant.
The high-concentration microorganism in the step (3) adopts Paracoccus aminovorans (A)Paracoccus aminovorans) LH-N40CGMCC number 6971.
The artificial simulated wastewater mainly comprises the following components: from (NH)4）2SO40.5g/L, glucose 1g/L, MgSO40.01g/L，K2HPO40.1g/L，FeSO4·7H20.05 g/L of O, 1L of distilled water and trace element liquid. The microelement liquid comprises the following main components: 50mg/L CoCl2·6H2O、150mg/L CaCl2、50mg/L CuSO4·5H2O、50mg/LZnSO4·7H2O、150mg/L FeSO4·7H2O、150mg/L MnSO4·2H2And O. In the film forming stage, the temperature is 10 ℃; pH 6.0; DO 0.5 mg/L. Practical wastewaters include, but are not limited to, the following industrial wastewaters: domestic sewage, coal chemical industry, synthetic ammonia, printing and dyeing, breeding and medicine.
Embodiment 6, a method for rapid biofilm formation of efficient denitrification microorganisms, comprising the following steps:
(1) filling biological fillers: adding a biological filler into an aerobic nitrification reactor, wherein the filling volume is 90 percent of the effective volume of the reactor;
(2) inoculating activated sludge: after activated sludge is inoculated into the aerobic nitrification reactor, carrying out stuffy aeration for 48 hours, wherein the inoculum size accounts for 70% of the effective volume of the reactor after filling;
(3) adding high-concentration denitrifying microorganisms: high-concentration denitrifying microorganisms are added into the external circulation device, and the concentration of the suspended matters in the bacteria liquid is 5000 mg/L.
(4) Film forming of the aerobic nitrification reactor: adding artificial simulation wastewater every 2d in the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 50% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 150 mg/L; high-concentration microorganisms enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in the biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate; during the period, the circulation device does not discharge mud, and no supernatant flows out;
(5) actual wastewater operation: continuously adding actual ammonia nitrogen-containing wastewater into the aerobic nitrification reactor for 14 days, wherein the ammonia nitrogen concentration is 200mg/L, and finishing biofilm formation when the ammonia nitrogen removal rate of effluent is continuously more than or equal to 95% for 7 days; during the period, the circulation device begins to discharge mud, and the supernatant liquid continuously flows out.
The biological fillers include but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers.
The activated sludge in the step (2) is taken from and not limited to denitrification sludge of an aerobic tank of a municipal sewage treatment plant.
The high-concentration microorganism in the step (3) adopts Paracoccus aminovorans (A)Paracoccus aminovorans) LH-N40CGMCC number 6971.
The artificial simulated wastewater mainly comprises the following components: from (NH)4）2SO45g/L, glucose 1g/L, MgSO40.02g/L，K2HPO40.3g/L，FeSO4·7H20.1 g/L of O, 1L of distilled water and trace element liquid. The microelement liquid comprises the following main components: 150mg/L CoCl2·6H2O、150mg/L CaCl2、50mg/L CuSO4·5H2O、50mg/LZnSO4·7H2O、50mg/L FeSO4·7H2O、150mg/L MnSO4·2H2And O. In the film forming stage, the temperature is 35 ℃; pH 10.0; DO 4.0 mg/L. Practical waste waters include, but are not limited to, the following rowsIndustrial wastewater: domestic sewage, coal chemical industry, synthetic ammonia, printing and dyeing, breeding and medicine.
Embodiment 7, a method for rapid biofilm formation of efficient denitrification microorganisms, the method comprises the following steps:
(1) filling biological fillers: adding a biological filler into an aerobic nitrification reactor, wherein the filling volume is 75 percent of the effective volume of the reactor;
(2) inoculating activated sludge: after activated sludge is inoculated into the aerobic nitrification reactor, stuffy aeration is carried out for 24 hours, and the inoculum size accounts for 60 percent of the effective volume of the reactor after filling;
(3) adding high-concentration denitrifying microorganisms: high-concentration denitrifying microorganisms are added into an external circulation device, and the concentration of the suspended matters in the bacteria liquid is 4000 mg/L.
(4) Film forming of the aerobic nitrification reactor: adding artificial simulation wastewater every 2d in the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 40% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 100 mg/L; high-concentration microorganisms enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in the biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate; during the period, the circulation device does not discharge mud, and no supernatant flows out;
(5) actual wastewater operation: continuously adding actual ammonia nitrogen-containing wastewater into the aerobic nitrification reactor for 10 days, wherein the ammonia nitrogen concentration is 150mg/L, and finishing biofilm formation when the removal rate of the ammonia nitrogen in the effluent is continuously 7 days or more than 95%; during the period, the circulation device begins to discharge mud, and the supernatant liquid continuously flows out.
The biological fillers include but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers.
The activated sludge in the step (2) is taken from and not limited to denitrification sludge of an aerobic tank of a municipal sewage treatment plant.
The step (3) isThe high concentration microorganism of (A) is Paracoccus aminovorans: (A)Paracoccus aminovorans) LH-N40CGMCC number 6971.
The artificial simulated wastewater mainly comprises the following components: from (NH)4）2SO42g/L, glucose 0.8g/L, MgSO40.015g/L，K2HPO40.2g/L，FeSO4·7H20.08 g/L of O, 1L of distilled water and trace element liquid. The microelement liquid comprises the following main components: 100mg/L CoCl2·6H2O、100mg/L CaCl2、25mg/L CuSO4·5H2O、100mg/LZnSO4·7H2O、120mg/L FeSO4·7H2O、110mg/L MnSO4·2H2And O. In the film forming stage, the temperature is 25 ℃; pH 8.0; DO 2.0 mg/L. Practical wastewaters include, but are not limited to, the following industrial wastewaters: domestic sewage, coal chemical industry, synthetic ammonia, printing and dyeing, breeding and medicine.
Claims (8)
1. A method for quickly forming a membrane by using efficient denitrification microorganisms is characterized in that the aerobic nitrification reactor is adopted for forming the membrane, and the specific method is as follows: adding a biological filler into an aerobic nitrification reactor, and internally connecting activated sludge into the aerobic nitrification reactor; adding artificial simulation wastewater every 1-2 d into the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 30-50% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 50-150 mg/L; high-concentration denitrifying microorganisms with the concentration of suspended matters of bacteria liquid of 3000-5000 mg/L enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in a biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate;
the method comprises the following specific steps:
(1) filling biological fillers: adding a biological filler into an aerobic nitrification reactor, wherein the filling volume is 60-90% of the effective volume of the reactor;
(2) inoculating activated sludge: after activated sludge is inoculated into the aerobic nitrification reactor, stuffy aeration is carried out for 24-48 h, and the inoculum size accounts for 50-70% of the effective volume of the reactor after filling with the filler;
(3) adding high-concentration denitrifying microorganisms: adding high-concentration denitrifying microorganisms into an external circulation device, wherein the concentration of suspended matters in the bacteria liquid is 3000-5000 mg/L;
(4) film forming of the aerobic nitrification reactor: adding artificial simulation wastewater every 1-2 d into the aerobic nitrification reactor, and controlling the temperature, dissolved oxygen and pH of the reactor until the wastewater accounts for 30-50% of the aerobic nitrification reactor, wherein the ammonia nitrogen concentration in the artificial simulation wastewater is controlled to be 50-150 mg/L; high-concentration denitrification microorganisms enter the aerobic nitrification reactor from the top through an external circulation pipeline, and form a solid-liquid flow from top to bottom in a biological filler area; meanwhile, bubbles formed by micropore aeration form gaseous flow from bottom to top in the biological filler area, the three are fully mixed in the biological filler area to form a fluidized state, and denitrification microorganisms are attached to or grow on the surface of the biological filler to gradually form a microorganism aggregate; during the period, the circulation device does not discharge mud, and no supernatant flows out;
(5) actual wastewater operation: continuously adding actual ammonia nitrogen-containing wastewater into the aerobic nitrification reactor for 7-14 days, wherein the ammonia nitrogen concentration is 100-200 mg/L, and finishing biofilm formation when the ammonia nitrogen removal rate of effluent is continuously more than or equal to 95% for 7 days; during the period, the circulation device begins to discharge mud, and the supernatant liquid continuously flows out.
2. The method for rapid biofilm formation by the efficient denitrification microorganisms as claimed in claim 1, which is characterized in that: the biological fillers include but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers.
3. The method for rapid biofilm formation by efficient denitrification microorganisms according to claim 1, wherein the activated sludge in the step (2) is taken from and not limited to the denitrification sludge of the aerobic tank of a municipal sewage treatment plant.
4. According to the rightThe method for rapid biofilm formation by the efficient denitrifying microorganisms of claim 1, wherein the high-concentration denitrifying microorganisms of step (3) adopt Paracoccus aminovorans (A)Paracoccus aminovorans) LH-N40CGMCC No. 6971.
5. The method for rapid biofilm formation by efficient denitrification microorganisms as claimed in claim 1, wherein the artificial simulated wastewater mainly comprises the following components: from (NH)4）2SO40.5-5 g/L, 0.5-1 g/L glucose, MgSO40.01～0.02g/L，K2HPO40.1～0.3g/L，FeSO4·7H20.05-0.1 g/L of O, 1L of distilled water and trace element liquid.
6. The method for rapid biofilm formation by efficient denitrification microorganisms as claimed in claim 5, wherein the microelement liquid mainly comprises the following components: 50-150 mg/L CoCl2·6H2O、50~150mg/L CaCl2、10~50mg/L CuSO4·5H2O、50~150mg/L ZnSO4·7H2O、50~150mg/L FeSO4·7H2O、50~150mg/L MnSO4·2H2O。
7. The method for rapid biofilm formation by the efficient denitrification microorganisms as claimed in claim 1, wherein in the biofilm formation stage, the temperature is 10-35 ℃, the pH is 6.0-10.0, and the DO is 0.5-4.0 mg/L.
8. The method for rapid biofilm formation by high-efficiency denitrification microorganisms as claimed in claim 1, wherein the actual wastewater comprises but is not limited to the following industrial wastewater: domestic sewage, coal chemical industry, synthetic ammonia, printing and dyeing, breeding and medicine.
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