CN112520858B - Method for improving biofilm formation efficiency and application - Google Patents

Method for improving biofilm formation efficiency and application Download PDF

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CN112520858B
CN112520858B CN201910878090.7A CN201910878090A CN112520858B CN 112520858 B CN112520858 B CN 112520858B CN 201910878090 A CN201910878090 A CN 201910878090A CN 112520858 B CN112520858 B CN 112520858B
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CN112520858A (en
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李庭刚
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Institute of Process Engineering of CAS
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/10—Solids, e.g. total solids [TS], total suspended solids [TSS] or volatile solids [VS]

Abstract

The invention relates to a method for improving biofilm formation efficiency and application, wherein the method comprises the following steps: inoculating activated sludge into a biomembrane reactor, feeding wastewater to ensure that the organic load of water inlet of the reactor is not higher than 0.5 g-COD/(g-VSS.d), and continuously operating, culturing and inducing until biomembrane biofilm formation is finished. The biofilm formation method can promote the formation of the biofilm regardless of the presence of the heterotropic mass transfer biofilm or the traditional cocurrent mass transfer biofilm, can realize rapid biofilm formation, can meet the requirement of rapidly starting the heterotropic mass transfer biofilm reactor or the traditional cocurrent mass transfer biofilm reactor, and has wide application range; the film forming method has the advantages of high film forming speed, simple operation, difficult film removal, no need of adding any medicament, no need of treating filler or carrier and high wastewater treatment efficiency.

Description

Method for improving biofilm formation efficiency and application
Technical Field
The invention belongs to the technical field of wastewater treatment, and particularly relates to a method for improving biofilm formation efficiency and application.
Background
With the continuous development of sewage treatment technology, the biofilm method is increasingly emphasized and widely applied due to various advantages and technical advancement of the biofilm method, such as impact resistance, strong adaptability, high biomass, long generation time, less residual sludge, strong treatment capacity, low power cost, convenient management and the like.
The Membrane Aeration Biomembrane Reactor (MABR) is a novel treatment system with coupling of a gas permeable membrane and a biomembrane, and two prominent characteristics of the reactor are functional activity stratification of the biomembrane caused by bubble-free aeration and anisotropic mass transfer of oxygen and a substrate. The MABR adopts a hydrophobic gas film to provide bubble-free aeration, enhances the mass transfer efficiency of oxygen and the high oxygen utilization rate (up to 100 percent), thereby reducing the treatment energy consumption, and avoids the gas stripping loss of volatile organic pollutants caused by the traditional bubble aeration or mechanical stirring aeration in the treatment process. Meanwhile, the membrane aeration biomembrane reactor adopts a hydrophobic gas membrane as a carrier for adhering the microbial biomembrane. Due to the functional activity layering of the heterotropic mass transfer biomembrane, the biomembrane can be layered into an aerobic/anoxic/anaerobic zone, and simultaneously, the carbon removal and denitrification are carried out in one biomembrane, thus having obvious advantages for treating high COD, degrading volatile organic pollutants and carrying out nitrification and denitrification biological denitrification and being a promising advanced environment-friendly technology.
The formation of the biofilm is a complex process, not all microorganisms can be successfully attached and grow on the breathable film to form the biofilm, particularly for MABR, the biofilm formation period is long, and the start is slow. Direct engineering attempts may result in high time and economic costs, and it is difficult to achieve rapid film formation and startup. Although the formation of natural biofilms has been extensively studied, it is economically unfeasible in large scale engineering applications due to the long time required to reach the immobilized biofilm biomass required per unit area/volume, a problem that has prompted the study of rapid biofilm formation methods.
CN105461083A discloses a suspended filler for a microorganism fast film hanging, which comprises the following components in parts by weight: 65-75 parts of high-density polyethylene, 5-15 parts of slaked lime, 5-20 parts of Dow powder activated carbon, 6-10 parts of light calcium carbonate, 3-5 parts of maleic anhydride, 0.2-0.6 part of dicumyl peroxide, 1.5-3 parts of gelatin, 1-2 parts of chitin, 0.8-2 parts of ferroferric oxide magnetic powder and 0.1-0.3 part of manganese-zinc ferrite; the density of the suspended filler for the rapid biofilm culturing of the microorganisms is 0.96-0.98g/cm 3. The filler has narrow application range and low applicability.
CN106430528A discloses a method for quickly starting a moving bed biofilm reactor under low temperature conditions. The method realizes the quick start of the moving bed biofilm reactor at low temperature by culturing and domesticating low-temperature microorganisms and preparing the filler containing a microorganism attachment layer. The method has the advantages of complex operation, too long domestication and culture time of the low-temperature microorganisms, and large addition, thereby increasing the treatment cost.
Therefore, aiming at the wastewater treatment process of the biofilm reactor, particularly for MABR (membrane activated biofilm reactor), in order to solve the key outstanding problems of difficult formation of the biofilm on the surface of a carrier, long biofilm formation period, easy shedding, narrow adaptability and the like in practical engineering and promote the efficient application of the biofilm method in wastewater bodies difficult to biofilm formation, the research of the biofilm method which is economic, short in biofilm formation time, firm in biofilm, strong in impact load resistance and good in treatment effect is of great significance.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for improving the biofilm culturing efficiency and application.
In order to achieve the purpose, the invention adopts the following technical scheme:
in one aspect, the present invention provides a method for improving biofilm culturing efficiency, comprising: inoculating activated sludge into a biomembrane reactor, feeding wastewater to ensure that the organic load of water inlet of the reactor is not higher than 0.5 g-COD/(g-VSS.d), and continuously operating, culturing and inducing until biomembrane biofilm formation is finished.
The rapid biofilm formation method can promote the formation of the biofilm regardless of the presence of the heterotropic mass transfer biofilm or the traditional cocurrent mass transfer biofilm, can meet the requirement of rapidly starting the heterotropic mass transfer biofilm reactor or the traditional cocurrent mass transfer biofilm reactor, and has wide application range; the film forming method has the advantages of high film forming speed, simple operation, difficult film removal, no need of adding any medicament, no need of treating filler or carrier and high wastewater treatment efficiency. The 0.5 g-COD/(g-VSS. d) means that the chemical oxygen demand per gram of volatile suspended solids per day is 0.5 g.
The biofilm culturing method can stimulate and induce microbes to secrete more extracellular polymers, particularly increase adhesive extracellular polymers, wrap cells, and facilitate the microbes to adhere to the surface of a filler, or promote the microbial cells to gather together to form a biofilm floccule, and can also better stabilize the biofilm structure.
Preferably, the activated sludge is cultured under acidic conditions for 12-24h, such as 12h, 15h, 18h, 20h, 21h, 22h, 23h or 24h, etc., prior to inoculation.
Preferably, the acidic condition means a pH value of 2 to 4, for example pH 2, pH 2.5, pH 3, pH 3.5 or pH 4, etc.
Preferably, the activated sludge is cultured under alkaline conditions for 12-24h, such as 12h, 15h, 18h, 20h, 21h, 22h, 23h or 24h, etc., prior to inoculation.
Preferably, the alkaline condition means a pH of 10 to 11, for example pH 10, pH 10.5 or pH 11, etc.
The activated sludge is inoculated after being cultured under the acidic condition or the alkaline condition, so that the improvement of the biofilm formation efficiency can be promoted.
Preferably, the biofilm reactor comprises a heterotropic mass transfer biofilm reactor or a cocurrent mass transfer biofilm reactor.
The heterotropic mass transfer biomembrane reactor is used for example as a membrane aeration biomembrane reactor, and the cocurrent mass transfer biomembrane reactor is used for example as a moving bed biomembrane reactor or an anaerobic contact oxidation tank.
Preferably, the packing of the heterotropic mass transfer biofilm reactor comprises a hydrophobic gas film. The hydrophobic gas film provides bubble-free aeration and provides a carrier for the attachment growth of the biological film, and the method is characterized in that bubble-free oxygen supply and the functional active stratification of the biological film caused by the anisotropic mass transfer of oxygen and a substrate are provided.
Preferably, the hydrophobic gas membrane comprises a polyethylene membrane, a polyamide membrane, a polyethersulfone membrane, a polypropylene membrane, or a polyvinylidene fluoride membrane.
Preferably, the hydrophobic gas film comprises an organic film or an inorganic film.
Preferably, the hydrophobic gas membrane comprises a hollow membrane or a flat sheet membrane.
Preferably, the filler of the cocurrent mass transfer biofilm reactor comprises elastic filler, soft filler, semi-soft filler and composite filler.
Preferably, the biomass concentration of the activated sludge is (2500-3500) mg-VSS/L, such as 2500mg-VSS/L, 2700mg-VSS/L, 2800mg-VSS/L, 2900mg-VSS/L, 3000mg-VSS/L, 3200mg-VSS/L, 3400mg-VSS/L or 3500mg-VSS/L and the like. 3500mg-VSS/L means that the content of volatile suspended solids in each liter of activated sludge is 3500 mg.
Preferably, the Reynolds number of the water stream in the biofilm reactor is 1500-2100, such as 1500, 1600, 1700, 11800, 1900, 2000, 2050 or 2100, etc.
The invention can maintain the Reynolds number by controlling the rising flow velocity (or the circulating flow velocity) of the reactor, ensure that the flow state in the reactor is a laminar flow region rather than a turbulent flow layer, and reduce the shearing force, thereby leading the biological membrane to be better attached and gathered on the filler (carrier) and playing the role of improving the membrane hanging efficiency of the biological membrane.
Preferably, the biofilm reactor is maintained under aerobic or anaerobic conditions, wherein the aerobic conditions refer to a dissolved oxygen concentration of 1.5-4mg/L, such as 1.5mg/L, 1.8mg/L, 2mg/L, 2.5mg/L, 2.8mg/L, 3mg/L, 3.5mg/L or 4mg/L, etc.
Preferably, the wastewater comprises any one of volatile organic wastewater, wastewater containing high ammonia nitrogen or wastewater containing high salt organic wastewater or a combination of at least two of the volatile organic wastewater, the wastewater containing high ammonia nitrogen or the wastewater containing high salt organic wastewater. Specifically, for example, toxic and intractable acetonitrile waste water, municipal waste water, industrial waste water, nitrogen-containing waste water, methyl methacrylate chemical waste water, and the like.
In a third aspect, the present invention provides the use of a method as described above in the treatment of wastewater.
The method can be applied to the degradation of volatile organic wastewater such as nitrogen-containing toxic organic nitrile compounds, the removal of high-concentration organic carbon pollutants and the denitrification of nitrification/denitrification (traditional nitrification/denitrification, shortcut nitrification-anaerobic ammonia oxidation) processes.
Compared with the prior art, the invention has the following beneficial effects:
the rapid biofilm formation method can promote the formation of the biofilm whether aiming at the heterotropic mass transfer biofilm or the traditional cocurrent mass transfer biofilm, can meet the requirement of rapidly starting the heterotropic mass transfer biofilm reactor or the traditional cocurrent mass transfer biofilm reactor, and has wide application range; the film forming method has the advantages of high film forming speed, simple operation, difficult film release, no need of adding any medicament, no need of treating filler or carrier and high wastewater treatment efficiency. Has high practical application value and is easy to popularize and use in engineering.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
Example 1
The embodiment provides a method for improving biofilm culturing efficiency, which specifically comprises the following steps:
the method of the invention is adopted to start the same-direction mass transfer biomembrane-Moving Bed Biomembrane Reactor (MBBR) to start the biofilm formation, firstly 1600mL of aerobic activated sludge with the concentration of 2500mg-VSS/L is inoculated into an MBBR system (the effective volume is 3.2L, semi-flexible filler (polypropylene, phi 120, Jiangsu Nantai environmental protection filler Co., Ltd.), the filling degree is 30 percent), the inlet water is municipal wastewater, the COD concentration of the inlet water is 410mg-COD/L, the Hydraulic Retention Time (HRT) is 6h, the organic load of the inlet water of the reactor is 0.41 g-COD/(g-VSS. d), the stirring speed in the reactor is controlled to be 120rpm, a microporous aerator is adopted to supply oxygen, the dissolved oxygen concentration (DO) is maintained to be 2-4mg/L (the DO fluctuates within the range of 2-4mg/L during the operation period), the pH value ranges from 6.8 to 7.4 (the pH value fluctuates within the range of 6.8-7.4 during the operation period), and culturing the induced biofilm by adopting a continuous operation mode. After the operation for 1 day, the formation of a layer of thin brown biofilm floc on the surface of the filler can be observed by naked eyes, at the moment, the operation of the reactor is stopped, all suspended sludge in the MBBR system is removed, the MBBR reactor is operated again, and the operation parameters are kept unchanged. And (5) running to the 7 th day, and successfully hanging the membrane.
Comparative example 1
The comparison example provides a biofilm formation method, and the specific content is different from that of the example 1 only in that the organic load of the reactor inlet water is 2 g-COD/(g-VSS.d), and other parameters are kept consistent.
Evaluation test:
the biofilm formed on the 7 th day of the operation of example 1 and comparative example 1 was evaluated (repeated 3 times) for biofilm biomass, biofilm thickness, COD removal rate, effluent SS concentration (mass of suspended solids in effluent per unit volume), extracellular polymer amount (mass of extracellular polymer in volatile suspended solids per unit mass), and adhesive extracellular polymer amount (mass of adhesive extracellular polymer in volatile suspended solids per unit mass), with the results shown in table 1.
TABLE 1
Figure BDA0002205004190000061
Figure BDA0002205004190000071
As can be seen from the data in Table 1: compared with the traditional biofilm culturing mode, the biofilm culturing efficiency of the embodiment 1 is higher, and the biofilm biomass and the biofilm thickness are obviously increased; the COD removal rate is more than 2.4 times of that of the traditional mode, and the SS concentration of the effluent is only 0.29 time of that of the traditional mode. The mass concentration of the extracellular polymer and the adhesive extracellular polymer in the biofilm biomass is 3.6 times and 5.3 times that of the traditional mode respectively, which shows that the invention is easy to secrete more extracellular polymers, particularly the adhesive extracellular polymer in the film hanging mode.
Example 2
The embodiment provides a method for improving biofilm culturing efficiency, which specifically comprises the following steps:
the method of the invention is adopted to start the homodromous mass transfer anaerobic biomembrane-anaerobic contact oxidation tank to start the biofilm formation, and firstly, 6L of sludge of the digestion tank with the concentration of 3500mg-VSS/L is inoculated into the anaerobic contact oxidation tank (the effective volume is 4.5 m)3Elastic filler (YCDT type, environmental protection filler Limited of Jiangsu Nantai), 45% filling degree), inlet water is methyl methacrylate MMA chemical wastewater, and raw water COD concentration is 6000 mg-COD/L. In the case, the influent COD concentration is 1500mg-COD/L, the Hydraulic Retention Time (HRT) is 6.7h, the influent Organic Load (OLR) of the reactor is 0.26 g-COD/(g-VSS. d), the stirring speed in the reactor is controlled at 120rpm, the pH value is controlled within the range of 6.8-7.4 (the pH value fluctuates within the range of 6.8-7.4 during the operation period), and the continuous operation mode is adopted to culture and induce the biofilm formation. After 3 days of operation, the formation of thin dark brown biomembrane flocs on the surface of 78% of the filler can be observed by naked eyes, at the moment, the operation of the reactor is stopped, all suspended sludge in the anaerobic contact oxidation pond system is removed, the anaerobic contact oxidation pond is operated again, and the operation parameters are kept unchanged. And (5) running to the 10 th day, and successfully hanging the membrane.
Comparative example 2
The comparison example provides a biofilm formation method, and the specific content is different from that of the example 2 only in that the organic load of the reactor inlet water is 2 g-COD/(g-VSS.d), and other parameters are kept consistent.
Evaluation test:
the biofilm formed on the 10 th day of the operation of example 2 and comparative example 2 was evaluated (repeated 3 times) for biofilm biomass, biofilm thickness, COD removal rate, effluent SS concentration (mass of suspended solids in effluent per unit volume), extracellular polymer amount (mass of extracellular polymer in volatile suspended solids per unit mass), and adhesive extracellular polymer amount (mass of adhesive extracellular polymer in volatile suspended solids per unit mass), with the results shown in table 2.
TABLE 2
Biofilm characteristics Example 2 Comparative example 2
Biofilm biomass (g-VSS/L) 1.27±0.07 0.35±0.03
Biofilm thickness (cm) 0.35±0.02 0.07±0.02
COD removal Rate (%) 87.0±4.8 31.2±1.7
SS concentration of effluent(mg-SS/L) 13.0±0.8 33.6±2.4
Extracellular Polymer quantity (mg/g-VSS) 115.7±6.9 33.6±2.1
Amount of adhesive extracellular Polymer (mg/g-VSS) 89.4±5.3 15.3±0.9
As can be seen from the data in Table 2: compared with the traditional biofilm culturing mode, the biofilm culturing efficiency is higher, and the biofilm biomass and the biofilm thickness are obviously increased in the embodiment 2; the COD removal rate is more than 2.7 times of that of the traditional mode, and the SS concentration of the effluent is only 0.39 time of that of the traditional mode. The mass concentration of the extracellular polymer and the adhesive extracellular polymer in the biofilm biomass is 3.4 times and 5.8 times that of the traditional mode respectively, which shows that the invention is easy to secrete more extracellular polymers, particularly the adhesive extracellular polymer in the film hanging mode.
Example 3
The embodiment provides a method for improving biofilm culturing efficiency, which specifically comprises the following steps:
the method for starting the anisotropic mass transfer biomembrane MABR to start the biofilm formation is adopted, firstly, 500mL of secondary sedimentation tank activated sludge with the concentration of 3000mg-VSS/L is inoculated into an MABR system (the effective volume is 1.54L, and the specific surface area of a membrane component is 84.5 m)2/m3) The inlet water is toxic and difficult-to-treat Acetonitrile (ACN) wastewater, the concentration of the inlet water is 314mg-ACN/L, the corresponding COD concentration is 500mg-COD/L, the Organic Load (OLR) of the reactor is 0.32 g-COD/(g-VSS.d), the ascending flow rate in the reactor is controlled to be 2.5cm/s, and the oxygen partial pressure across the membrane is 22-28kPa, and the oxygen partial pressure is 14-17mL-O2Flow rate/min provides bubble-free aeration to maintain liquid phase dissolutionOxygen (DO) concentration is 1.5-3mg/L (DO fluctuates within the range of 1.5-3mg/L during the operation period), pH value is 6.5-7.4 (pH value fluctuates within the range of 6.5-7.4 during the operation period), and continuous operation mode is adopted to culture and induce biofilm formation. After 2 days of operation, a layer of thin faint yellow biomembrane flocs formed on the surface of the hollow fiber membrane (polypropylene hydrophobic membrane, Hangzhou Kai macromembrane technology, Inc.) can be observed by naked eyes, then the operation of the reactor is stopped, all suspended sludge in the MABR system is removed, the reactor is operated again, and the operation parameters are kept unchanged. And (5) running to the 9 th day, and successfully hanging the membrane.
Comparative example 3
The comparison example provides a biofilm formation method, and the specific content is different from that of the example 3 only in that the organic load of the reactor inlet water is 2 g-COD/(g-VSS.d), and other parameters are kept consistent.
Evaluation test:
the biofilm formed on the 9 th day of the operation of example 3 and comparative example 3 was evaluated (repeated 3 times) for biofilm biomass, biofilm thickness, COD removal rate, effluent SS concentration (mass of suspended solids in effluent per unit volume), extracellular polymer amount (mass of extracellular polymer in volatile suspended solids per unit mass), and adhesive extracellular polymer amount (mass of adhesive extracellular polymer in volatile suspended solids per unit mass), with the results shown in table 3.
TABLE 3
Biofilm characteristics Example 3 Comparative example 3
Biofilm biomass (g-VSS/L) 1.02±0.05 0.30±0.02
Biofilm thickness (cm) 0.40±0.03 0.06±0.01
COD removal Rate (%) 82.0±6.0 34.6±1.7
The SS concentration of effluent (mg-SS/L) 21.0±0.8 43.1±3.5
Extracellular Polymer quantity (mg/g-VSS) 134.9±7.8 37.2±2.1
Amount of adhesive extracellular Polymer (mg/g-VSS) 108.7±5.7 16.1±0.8
As can be seen from the data in Table 3: compared with the traditional biofilm culturing mode, the biofilm culturing efficiency is higher, and the biofilm biomass and the biofilm thickness are obviously increased in the embodiment 3; the COD removal rate is more than 2.3 times of that of the traditional mode, and the SS concentration of the effluent is only 0.48 time of that of the traditional mode. The mass concentration of the extracellular polymer and the adhesive extracellular polymer in the biofilm biomass is 3.6 times and 6.8 times that of the traditional mode respectively, which shows that the invention is easy to secrete more extracellular polymers, particularly the adhesive extracellular polymer in the film hanging mode.
Example 4
The present example provides a method for improving biofilm culturing efficiency, which is different from example 3 only in that the activated sludge in the secondary sedimentation tank is cultured for 12 hours under the condition of pH 3 before inoculation, and other conditions are kept unchanged, and the continuous operation mode is adopted to culture and induce biofilm culturing. After the operation for 2 days, a layer of thin faint yellow biomembrane flocs formed on the surface of the hollow fiber membrane can be observed by naked eyes, then the operation of the reactor is stopped, all suspended sludge in the MABR system is removed, the reactor is operated again, and the operation parameters are kept unchanged. And (5) running to the 6 th day, and successfully hanging the membrane.
Evaluation test:
the biofilm formed on the 6 th day of operation of example 4 was evaluated (repeated 3 times) for several indicators of biofilm biomass, biofilm thickness, biofilm surface coverage, COD removal rate, effluent SS concentration (mass of suspended solids in effluent water per unit volume), extracellular polymer amount (mass of extracellular polymer in volatile suspended solids per unit mass), and adhesive extracellular polymer amount (mass of adhesive extracellular polymer in volatile suspended solids per unit mass), and compared with the data of example 3, with the results shown in table 4.
TABLE 4
Biofilm characteristics Example 3 Example 4
Biofilm biomass (g-VSS/L) 1.02±0.05 1.19±0.08
Biofilm thickness (cm) 0.40±0.03 0.46±0.02
COD removal Rate (%) 82.0±6.0 91.3±5.4
The SS concentration of effluent (mg-SS/L) 21.0±0.8 17.6±1.3
Extracellular Polymer quantity (mg/g-VSS) 134.9±7.8 159.1±7.9
Amount of adhesive extracellular Polymer (mg/g-VSS) 108.7±5.7 132.6±6.5
From the data in table 4, it can be seen that: example 4 compared with example 3, the biofilm hanging efficiency is further improved, and the biofilm biomass and the biofilm thickness are further increased; the COD removal rate was 1.1 times or more that of example 3, and the effluent SS concentration was only 0.84 times that of example 3. The mass concentrations of the extracellular polymeric substance and the adhesive extracellular polymeric substance in the biofilm biomass were 1.2 times and 1.2 times, respectively, that of example 3.
Example 5
The present example provides a method for improving biofilm culturing efficiency, and the specific content is different from example 3 only in that the secondary sedimentation tank is cultured for 12h under the condition of pH 11 before activated sludge inoculation, other conditions are kept unchanged, and a continuous operation mode is adopted to culture and induce biofilm culturing. After 2 days of operation, a layer of thin faint yellow biomembrane floc is observed to be formed on the surface of the hollow fiber membrane by naked eyes, then the operation of the reactor is stopped, all suspended sludge in the MABR system is removed, the reactor is operated again, and the operation parameters are kept unchanged. And (5) running to the 7 th day, and successfully hanging the membrane.
Evaluation test:
the biofilm formed on day 7 of the run of example 5 was evaluated (repeated 3 times) for several indicators of biofilm biomass, biofilm thickness, COD removal rate, effluent SS concentration (mass of suspended solids in effluent per unit volume), extracellular polymer amount (mass of extracellular polymer in volatile suspended solids per unit mass), and adhesive extracellular polymer amount (mass of adhesive extracellular polymer in volatile suspended solids per unit mass) and compared with the data of example 3, with the results shown in table 5.
TABLE 5
Biofilm characteristics Example 3 Example 5
Biofilm biomass (g-VSS/L) 1.02±0.05 1.13±0.06
Biofilm thickness (cm) 0.40±0.03 0.43±0.03
COD removal Rate (%) 82.0±6.0 89.2±3.5
The SS concentration of effluent (mg-SS/L) 21.0±0.8 18.9±1.5
Extracellular Polymer quantity (mg/g-VSS) 134.9±7.8 151.3±7.6
Amount of adhesive extracellular Polymer (mg/g-VSS) 108.7±5.7 126.1±6.4
From the data in table 5, it can be seen that: compared with example 3, the biofilm hanging efficiency of example 5 is further improved, and the biofilm biomass and the biofilm thickness are further increased; the COD removal rate was 1.1 times or more that of example 3, and the effluent SS concentration was only 0.90 times that of example 3. The mass concentrations of extracellular and adhesive extracellular polymers in biofilm biomass were 1.1-fold and 1.2-fold, respectively, that of example 3.
The applicant states that the present invention is illustrated by the above examples to describe a method and application of the present invention for improving biofilm culturing efficiency, but the present invention is not limited to the above examples, which does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modifications of the present invention, equivalent substitutions of the raw materials of the product of the present invention, and the addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are all within the protection scope of the present invention.
It should be noted that, in the above embodiments, the various features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, the present invention does not separately describe various possible combinations.

Claims (9)

1. A method for improving biofilm culturing efficiency, which is characterized by comprising the following steps: inoculating activated sludge into a biomembrane reactor, feeding wastewater to ensure that the organic load of water inlet of the reactor is not higher than 0.5 g-COD/(g-VSS.d), and continuously operating, culturing and inducing until biomembrane biofilm formation is finished;
Culturing the activated sludge for 12-24h under an acidic condition with the pH value of 2-4 or culturing for 12-24h under an alkaline condition with the pH value of 10-11 before inoculation;
the biomembrane reactor is a membrane aeration biomembrane reactor;
the filler of the membrane aeration biomembrane reactor is a hydrophobic gas membrane.
2. The method of claim 1, wherein the hydrophobic gas membrane comprises a polyethylene membrane, a polyamide membrane, a polyethersulfone membrane, a polypropylene membrane, or a polyvinylidene fluoride membrane.
3. The method of claim 1, wherein the hydrophobic gas film comprises an organic film or an inorganic film.
4. The method of claim 1, wherein the hydrophobic gas membrane comprises a hollow membrane or a flat sheet membrane.
5. The method as claimed in claim 1, wherein the biomass concentration of the activated sludge is (2500-3500) mg-VSS/L.
6. The method of claim 1 wherein the reynolds number of the water stream in the biofilm reactor is 1500-.
7. The method of claim 1, wherein the biofilm reactor is maintained under aerobic conditions, wherein the aerobic conditions are dissolved oxygen concentrations of 1.5 to 4 mg/L.
8. The method of claim 1, wherein the wastewater comprises any one of or a combination of at least two of volatile organic wastewater, high ammonia nitrogen-containing wastewater, or high salt organic wastewater.
9. Use of a method according to any one of claims 1 to 8 in the treatment of waste water.
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