CN102219297A - Rapid culture method of aerobic granular sludge - Google Patents

Rapid culture method of aerobic granular sludge Download PDF

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CN102219297A
CN102219297A CN2010101493864A CN201010149386A CN102219297A CN 102219297 A CN102219297 A CN 102219297A CN 2010101493864 A CN2010101493864 A CN 2010101493864A CN 201010149386 A CN201010149386 A CN 201010149386A CN 102219297 A CN102219297 A CN 102219297A
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reactor
water
aerobic particle
particle mud
culture process
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郑俊华
刘永强
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Fudan University
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Abstract

Belonging to the technical field of biological wastewater treatment, the invention specifically relates to a rapid culture method of aerobic granular sludge. The method comprises the steps of: inoculating activated sludge in a sequencing batch reactor; pumping waste water into the reactor through a water inlet at the bottom of the reactor; conducting an aeration operation through an aeration device located at the bottom of the reactor; setting a precipitation time and bringing the reactor into a precipitation process; discharging the waste water above a water outlet out of the reactor by a drainage pump. Wherein, with a precipitation time longer than 0 but shorter than 15 minutes and an organic loading higher than 1.5g 1<-1> d<-1>, the steps between water inletting and water discharging repeat periodically in the reactor. The operation period may range from 0.5-12 hours, the time for water inletting and water discharging may be in a range of from 1 to 120 minutes, and the aeration intensity can be 0.1-5vvm. The method of the invention can effectively shorten the culture time of granular sludge, and the aerobic granular sludge generated by the rapid culture method enjoys a capability to maintain long-term stable operation.

Description

A kind of fast culture process of aerobic particle mud
Technical field
The invention belongs to the biological wastewater treatment technology field, be specifically related to a kind of fast culture process of aerobic particle mud.
Background technology
Activated sludge process is the dirty water living creature processing technique of present widespread usage, but traditional activated sludge process often has treatment effect not good, and the settling property difference influences effluent quality, and problems such as sludge bulking take place easily.And aerobic particle mud is emerging in recent years a kind of novel wastewater efficient biologic treating technique.Compare with traditional activated sludge process, the aerobic particle mud technology has better settling property because of it, higher biomass interception (sludge concentration) in the reactor, higher processing power, and accessible sewage type is more various, and poor environment is had stronger resistivity and has fabulous application prospect.
With anaerobic grain sludge at flow lifting type anaerobic biological reactor (Up-flow Anaerobic Sludge Bed, UASB) formation was compared with start time, (Sequencing Batch Reactor SBR) can lack much the formation in and start time aerobic particle mud at sequencing batch reactor.Even if but like this, utilizing the synthetic wastewater of degraded easily to cultivate sophisticated aerobic particle mud in the laboratory still needs time about 1 month.And support particulate and reach half a year or more of a specified duration start time cultivating in pilot reactor with actual cities waste water or trade effluent.This becomes the restriction aerobic particle mud and further moves towards one of bottleneck of practical application.In the former research, it is found that in the cultivation of aerobic particle mud and add into calcium ion (Ca 2+) can reinforcing particle the formation of mud shortening incubation time, but this cultural method still needs the general 16 days time.Although in active sludge, add the also further formation of reinforcing particle of method of the purebred bacterium with self coagulation ability, just can turn out the aerobic particle mud of median size 446um in this way in 8 days, but this in actual applications method need be carried out a large amount of purebred microbial culture earlier, the operation steps complexity, success ratio is difficult to guarantee, poor operability, actual application value is lower.
Therefore, the further promotion start time aerobic particle mud broad application that shortens aerobic granular sludge reactor is very important problem, also has very important realistic meaning and economic worth.
Summary of the invention
The object of the present invention is to provide a kind of fast culture process of aerobic particle mud, this fast culture process can shorten effectively to be cultivated the time that aerobic particle mud needs, and overcomes the bottleneck of aerobic particle mud in actual applying.
The invention discloses a kind of fast culture process of aerobic particle mud, this method comprises the following steps:
(1) inoculation: inoculation of activated-sludge in sequencing batch reactor;
(2) water inlet: the water-in of sewage by the sequencing batch reactor bottom pumped in the reactor;
(3) aeration: the aerating apparatus by the reactor bottom setting carries out the aeration operation, and the sewage of mud and access reactor is fully reacted;
(4) precipitation: sedimentation time is set, and reactor enters the sludge settling operation;
(5) draining: the sewage that is positioned at the above position of water outlet is discharged reactor by wet-pit;
Wherein, sedimentation time is greater than 0 less than 15 minutes, and organic loading is higher than 1.5g l -1d -1, step (2) to (5) is moved repeatedly by the cycle in reactor.
Between step (2) and step (3), can increase step: muddy water is left standstill or mix, make reactor be in anoxic or anaerobic state.
The fast culture process of a kind of aerobic particle mud of the present invention is at a reactor that moves in the sequence batch (mode (Sequencing Batch Reactor, SBR) finish in, as shown in Figure 1, the device that relates to of aerobic particle mud fast culture process is by aeration air pump 1, gas meter 2, sequencing batch reactor SBR3, intake pump 4, water-in 5, system time controller 6, goes out water pump 7, water outlet 8 etc. and constitute.In the main body reactor, water inlet enters into reactor 3 by intake pump 4 by water-in 5, and gas is provided by air pump 1 and controls its flows by gas meter 2.Sewage is through the aerobic aeration reaction, and mud is according to the time precipitation of setting, and the sewage of handling is afterwards discharged reactor by going out water pump 7 via water outlet 8, and this cover system is controlled the switch activated of each pump by time controller 6.
By the cycle repeatable operation, the operational cycle can be looked the influent quality decision in reactor in above step water inlet-draining.Adopt less than 15 minutes sedimentation time during the operation of this method and be higher than 1.5g l -1d -1The mud organic loading, and the operational cycle scope can be 0.5-12 hour, flooding time and water discharge time scope can be at 1-120 minutes, aeration intensity can be at 0.1-5vvm, anaerobism or anoxic stage before the aeration phase are not essential.Above indication active sludge is meant the various mud that are widely used in disposing of sewage.
Further, the invention provides a kind of method of keeping the long-term stability operation of aerobic granular sludge reactor, this method main points comprise:
A. reduce organic loading to 10g l by chemical oxygen demand (COD) (COD) concentration of prolong operation cycle or reduction water inlet or the method for minimizing exchange ratio -1d -1Below;
Sludge settling time dimension during B. with steady-state operation was held in below 10 minutes.
In the present invention, the laboratory condition easy synthetic wastewater of degrading of utilization down just can be turned out aerobic particle mud in 1 day, and reactor just can reach steady operational status in the 1-2 time-of-week, and the aerobic particle mud of this fast culture has the ability of the operation of maintaining a long-term stability.In pilot scale research, reactor can aerobic particle mud just occur in 14 days, and particle characteristic reaches stable state within 30 days, and system enters steady state and shows the ability of operation steady in a long-term at 50 days left and right sides reactor performances of operation.
The advantage that the method that the present invention relates to is compared with general method is, the method that progressively reduces sedimentation time and fixed reactor organic loading that mostly adopts traditional method for culturing aerobic granular sludge realizes the cultivation of aerobic particle mud, is higher reactor organic loading and the relatively shorter sedimentation time of fixed and the method that relates among the present invention adopts when reactor start-up.
The advantage that the method that the present invention relates to is compared with general method is that also the higher reactor organic loading that adopts can not cause disadvantageous effect to the aerobic sludge pelletising process when reactor start-up.
The advantage that the method that the present invention relates to is compared with general method is that also the time that mud is finished granulating to be needed shortens dramatically, and only needs 1-2 week.
Description of drawings
Fig. 1 aerobic particle mud fast culture device synoptic diagram
Number in the figure is: 1. aeration air pump; 2. gas meter; 3. sequencing batch reactor SBR; 4. intake pump; 5. water-in; 6. system time controller; 7. go out water pump; 8. water outlet
Embodiment
The following examples are to further specify of the present invention, rather than limit the scope of the invention.
Embodiment 1: utilize synthetic wastewater to cultivate aerobic grain in the laboratory
Material and method
Reactor start-up
Mud enters in the reactor after the domestication through synthetic water distribution in 4 days from general municipal wastewater treatment plant.The working volume 2.0L of reactor is with the mode periodic duty of sequence batch (.Synthetic sewage pumps in the reactor by the water-in of reactor bottom, and flooding time is 5 minutes phases weekly.Aeration realizes that via the reactor bottom air inlet reactor carries out biochemical reaction by air pump.After aeration stopped, reactor assembly entered precipitate phase.Adopt 5 minutes short sedimentation time that the good mud of settling property is trapped in the reactor.Reacted water outlet is discharged via the water outlet that is positioned at reactor middle part (exchange is than 50%) by wet-pit, and water discharge time is 5 minutes phases weekly.System's operation realizes control by a time controller.Present embodiment relates to the operation of 7 reactors, and the operating parameter of each reactor sees following table 1 for details.
The operating parameter of table 17 reactor
Figure GSA00000079269700031
Figure GSA00000079269700041
Synthetic sewage becomes to be grouped into
The synthetic sewage that adopts in the present embodiment mainly is grouped into by following one-tenth: COD (CH 3COONa) 1000mg l -1, NH 4Cl50mg l -1, K 2HPO 445mg l -1, CaCl 22H 2O 30mg l -1, MgSO 47H 2O 25mg l -1, FeSO 47H 2O 20mg l -1, H 3BO 30.05mg l -1, ZnCl 20.05mg l -1, CuCl 20.03mg l -1, MnSO 4H 2O 0.05mg l -1, (NH 4) 6Mo 7O 244H 2O 0.05mg l -1, AlCl 30.05mg l -1, CoCl 26H 2O 0.05mg l -1, NiCl 20.05mg l -1
Analytical procedure
Sludge volume index (SVI) and sludge concentration (MLSS) are measured according to standard method, the granule sludge size is by laser particle dimension analysis system (model: Malvern MasterSizer 2600) and by microscope (model: combining image analytical system (model: Image-Pro Plus Olympus SZX9), V4.0, Media Cybernetics) measure.The morphological analysis of granule sludge is finished by above-mentioned image analysis system and taken pictures.
In 7 reactors that present embodiment relates to, granule sludge formed within 1 day all.There is not flco mud with regard to finding to have only granule sludge in this reactor when in fact, some reactor is observed mud with image analysis system after starting 6 hours.The granule sludge concentration that forms has been passed through lag period and exponential growth after date, has just reached steady state in 12 days.Up to the present, compare with existing report, the mud quick particle speed that present embodiment relates to is the fastest, and the time that the while aerobic granular sludge reactor reaches steady state to be needed also is the shortest.Discover that the aerobic particle mud of cultivating by this fast culture technology that the present invention relates to all can keep steady state in the working time of half a year, and good processing power and settling property.
Embodiment 2: utilize the municipal effluent rapidly cultivating aerobic particle sludge in the pilot scale reactor
Research final purpose for aerobic particle mud is to realize the practical application of aerobic particle mud.But the general main laboratory scale cultivation that also is based on of the research of aerobic particle mud up to the present, the research under the above practical situation of pilot scale or pilot scale also seldom.Simultaneously, there are the factors that influence the aerobic particle mud cultivation in the above scale of pilot scale or pilot scale more, so under the above scale condition of pilot scale or pilot scale, the incubation time that needs is often longer than laboratory culture.At present, the report pilot scale is cultivated in the example of aerobic particle mud, and the granule sludge that needs is cultivated and reached sometimes start time more than a year, and this has brought very big difficulty for further practical application popularization of aerobic particle mud.The technological method that the present invention relates to can be applied to the fast culture and the steady running of the aerobic particle mud under the practical situation too.
The reactor body that present embodiment relates to is a diameter 20cm, the synthetic glass post of effective volume 40L.Reactor adopts the sequence batch (mode to move, and technology comprises into four steps such as water, aeration, precipitation and water outlet, is a cycle of operation with 4 hours.Reactor is intake from the bottom, and flooding time is 5 minutes.Reactor is by wet-pit middle part draining, and water discharge time is 5 minutes.Gas is provided by air pump from reactor bottom.
Concrete processing step is:
1. will inoculate in this reactor from the active sludge of same waste plant, the sludge concentration of inoculation post-reactor is at 4-6g/L.Sewage was squeezed into reactor from reactor bottom by water pump in 5 minutes;
2. the aerating apparatus by the reactor bottom setting after water inlet is finished begins to carry out the aeration operation, and the sewage of mud and access reactor is fully reacted;
3. after aeration finished, reactor entered the sludge settling time.Sedimentation time is set at 15 minutes, and short sedimentation time produces bigger selective pressure, and good mud is trapped in the reactor to make settling property, and the mud of settling property difference will be discharged from reactor in drainage procedure;
4. post precipitation, the sewage that is positioned at the above position of water outlet is discharged reactor by wet-pit, also the mud of settling property difference is discharged simultaneously.
Adopt above technology, about 30 days, the characteristic index of granule sludge all enters steady state to reactor at seed sludge.About 50 days, reactor performance also enters stable operation stage such as removing of ammonia nitrogen.In steady operation period, aerobic particle mud concentration is about 6-8g l in the reactor -1, sludge volume index is 30-40ml g -1, granule sludge median size 1800 μ m.Simultaneously, it is 85-95% that COD removes efficient, NH 4 +It is more than 95% that-N removes efficient.This result shows that the aerobic grain that this fast culture technology cultivates out has higher degrading activity and stability.In working time how half a year afterwards, this system shows good steady running ability.
Different with traditional cultural method is that it (is greater than 1.5g l in this example that this technology adopts short sedimentation time (being to be less than 15 minutes in this example) and bigger sludge loading simultaneously -1d -1) selective pressure of coming reinforcement sludge.The fast culture technology that the present invention relates to is just turned out the fabulous granule sludge of the superior processing power of settling property in one month time, shortened greatly and cultivated the time that aerobic particle mud needs.

Claims (9)

1. the fast culture process of an aerobic particle mud is characterized in that, this method comprises the following steps:
(1) inoculation: inoculation of activated-sludge in sequencing batch reactor;
(2) water inlet: the water-in of sewage by the sequencing batch reactor bottom pumped in the reactor;
(3) aeration: the aerating apparatus by the reactor bottom setting carries out the aeration operation, and the sewage of mud and access reactor is fully reacted;
(4) precipitation: sedimentation time is set, and reactor enters the sludge settling operation;
(5) draining: the sewage that is positioned at the above position of water outlet is discharged reactor by wet-pit;
Wherein, sedimentation time is greater than 0 less than 15 minutes, and organic loading is higher than 1.5gl -1d -1, step (2) to (5) is moved repeatedly by the cycle in reactor.
2. the fast culture process of a kind of aerobic particle mud according to claim 1 is characterized in that, increases by a step between step (2) and step (3): muddy water is left standstill or mix, make reactor be in anoxic or anaerobic state.
3. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2, it is characterized in that: the cycle of operation of reactor is 0.5-12 hour.
4. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2 is characterized in that: flooding time and water discharge time during the reactor operation are 1-120 minute.
5. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2, it is characterized in that: aeration intensity is 0.1-5vvm.
6. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2 is characterized in that: make the organic loading of reactor be higher than 1.5g l by the red-tape operati cycle -1d -1
7. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2 is characterized in that: be higher than 1.5g l by the control exchange than the organic loading that makes reactor -1d -1
8. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2 is characterized in that: sedimentation time greater than 0 minute less than 10 minutes.
9. the fast culture process of a kind of aerobic particle mud according to claim 1 and 2, it is characterized in that: the organic loading of reactor is lower than 10g l -1d -1
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CN102583722A (en) * 2012-03-12 2012-07-18 北京化工大学 Immobilized-cultivation method for aerobic granular sludge
CN102583721A (en) * 2012-03-12 2012-07-18 北京化工大学 Method for culturing load fluctuation tolerant volatile aerobic granule sludge used for low-concentration wastewater
CN103214087A (en) * 2013-04-25 2013-07-24 同济大学 Method for enriching phosphorus by using aerobic granular sludge
CN104556362A (en) * 2013-10-11 2015-04-29 中国石油化工股份有限公司 Aerobic granular sludge culture method
CN105481085A (en) * 2015-08-21 2016-04-13 仲恺农业工程学院 Method for rapidly culturing aerobic granular sludge by controlling flow field and apparatus
CN106115908A (en) * 2016-08-29 2016-11-16 武汉东川自来水科技开发有限公司 A kind of cultural method of induced aerobic particle mud
CN107601655A (en) * 2017-09-20 2018-01-19 苏州科技大学 Method for culturing aerobic granular sludge and culture apparatus
CN108191049A (en) * 2018-01-30 2018-06-22 上海禾元环保集团有限公司 It cultivates the method for aerobic particle mud and cultivates its device
CN109052663A (en) * 2018-08-27 2018-12-21 华东理工大学 A kind of salt tolerant removes carbon microbe granular material fast breeding method
CN110330099A (en) * 2019-08-14 2019-10-15 重庆市生态环境科学研究院 A kind of method for culturing aerobic granular sludge and culture apparatus
CN111606414A (en) * 2020-05-29 2020-09-01 西安工程大学 SBR reactor for stably culturing aerobic granular sludge and operation method thereof
CN112520858A (en) * 2019-09-17 2021-03-19 中国科学院过程工程研究所 Method for improving biofilm formation efficiency and application
CN112939210A (en) * 2021-02-06 2021-06-11 重庆大学 Integrated sewage treatment device and method utilizing carbon capture and aerobic granular sludge
CN113184985A (en) * 2021-05-19 2021-07-30 西南交通大学 Method for promoting stable operation of aerobic granular sludge

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CN102583721A (en) * 2012-03-12 2012-07-18 北京化工大学 Method for culturing load fluctuation tolerant volatile aerobic granule sludge used for low-concentration wastewater
CN102583721B (en) * 2012-03-12 2013-08-21 北京化工大学 Method for culturing load fluctuation tolerant volatile aerobic granule sludge used for low-concentration wastewater
CN102583722B (en) * 2012-03-12 2014-03-26 北京化工大学 Immobilized-cultivation method for aerobic granular sludge
CN102583722A (en) * 2012-03-12 2012-07-18 北京化工大学 Immobilized-cultivation method for aerobic granular sludge
CN103214087A (en) * 2013-04-25 2013-07-24 同济大学 Method for enriching phosphorus by using aerobic granular sludge
CN104556362A (en) * 2013-10-11 2015-04-29 中国石油化工股份有限公司 Aerobic granular sludge culture method
CN104556362B (en) * 2013-10-11 2017-02-01 中国石油化工股份有限公司 Aerobic granular sludge culture method
CN105481085A (en) * 2015-08-21 2016-04-13 仲恺农业工程学院 Method for rapidly culturing aerobic granular sludge by controlling flow field and apparatus
CN106115908B (en) * 2016-08-29 2019-05-31 武汉东川自来水科技开发有限公司 A kind of cultural method of induction type aerobic particle mud
CN106115908A (en) * 2016-08-29 2016-11-16 武汉东川自来水科技开发有限公司 A kind of cultural method of induced aerobic particle mud
CN107601655A (en) * 2017-09-20 2018-01-19 苏州科技大学 Method for culturing aerobic granular sludge and culture apparatus
CN108191049A (en) * 2018-01-30 2018-06-22 上海禾元环保集团有限公司 It cultivates the method for aerobic particle mud and cultivates its device
CN109052663A (en) * 2018-08-27 2018-12-21 华东理工大学 A kind of salt tolerant removes carbon microbe granular material fast breeding method
CN109052663B (en) * 2018-08-27 2020-08-28 华东理工大学 Rapid cultivation method of salt-tolerant carbon-removal microbial granular material
CN110330099A (en) * 2019-08-14 2019-10-15 重庆市生态环境科学研究院 A kind of method for culturing aerobic granular sludge and culture apparatus
CN112520858A (en) * 2019-09-17 2021-03-19 中国科学院过程工程研究所 Method for improving biofilm formation efficiency and application
CN112520858B (en) * 2019-09-17 2022-06-28 中国科学院过程工程研究所 Method for improving biofilm formation efficiency and application
CN111606414A (en) * 2020-05-29 2020-09-01 西安工程大学 SBR reactor for stably culturing aerobic granular sludge and operation method thereof
CN111606414B (en) * 2020-05-29 2023-11-17 西安工程大学 SBR reactor for stably culturing aerobic granular sludge and operation method thereof
CN112939210A (en) * 2021-02-06 2021-06-11 重庆大学 Integrated sewage treatment device and method utilizing carbon capture and aerobic granular sludge
CN113184985A (en) * 2021-05-19 2021-07-30 西南交通大学 Method for promoting stable operation of aerobic granular sludge
CN113184985B (en) * 2021-05-19 2022-07-12 西南交通大学 Method for promoting stable operation of aerobic granular sludge

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