CN101265458A - Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation - Google Patents
Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation Download PDFInfo
- Publication number
- CN101265458A CN101265458A CNA2008100238758A CN200810023875A CN101265458A CN 101265458 A CN101265458 A CN 101265458A CN A2008100238758 A CNA2008100238758 A CN A2008100238758A CN 200810023875 A CN200810023875 A CN 200810023875A CN 101265458 A CN101265458 A CN 101265458A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- bacteria
- strong film
- film
- sewage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Bacteria which have strong ability to form a membrane are prepared and a method for strengthening sewage denitrification is provided. The invention relates to the preparation of bacteria which have strong ability to form a biomembrane and a technique for strengthening sewage denitrification by using the bacteria. The bacteria which have strong ability to form a biomembrane are separated and screened from a sewage and waste water treatment system and the biomembrane of nature water bodies. In the commissioning period of the sewage treatment system, the bacteria which have strong ability to form a biomembrane, nitrobacteria and denitrifying bacteria are added, total addition (green weight) accounts for 0.1-2.0% (green weight of the bacteria) of sewage volume, the weight percentages among the bacteria which have strong ability to form a biomembrane: nitrobacteria and nitrite bacteria: denitrifying bacteria are (1-3): (2-5): (1-3), and then denitrification can be effectively conducted. The addition of the bacteria which have strong ability to form a biomembrane enable a carrier to form a thicker biomembrane, thereby improving the denitrification efficiency of the sewage treatment system; the functional bacteria are fixed on the biomembrane, thereby reducing the addition times of the bacteria, reducing cost and simplifying management.
Description
Technical field
The present invention relates to sewage disposal technology, specially refer to the technology of making and utilize this bacterium strengthening sewage denitrification that microbial film forms the strong bacterium of ability.
Background technology
Sequencing batch reactor integrates treatment tank and settling tank, and it is little to have floor space, less investment, and capacity of resisting impact load is strong, the automatic controlling level height, need not characteristics such as personal management, is one of first-selected treatment process of disperseing the resident living sewage disposal.But there is the not high problem of biological denitrificaion efficient in conventional sequencing batch reactor, along with improving constantly of China's sewage drainage standard, do not increasing cost of investment and not on the basis with complex treatment processization, method and the technology of strengthening sequencing batch reactor denitrogenation dephosphorizing function have crucial meaning as far as possible.
But add the degradation capability of the biological reinforcing technology enhancement process system of function yeast to treatment system, improve degradation rate specific pollutants.Wang Jianfangs etc. add effective microbe (EM) the bacterium liquid of being made up of tens kinds of bacteriums in sequencing batch reactor (SBR) and sequencing batch biofilm reactor (SBBR), make the COD in the sanitary sewage
Cr, principal pollutant such as nitrogen, phosphorus all obtain removal effect preferably, but because bacterial classification runs off with water outlet, cause the scrubbing effect periodically to descend, need to reactor intercycle throwing bacterium.Though it is simple regularly to add the method for function yeast to treatment system, and can obtain treatment effect preferably, has increased processing cost and management difficulty.
In order to reduce the loss that adds thalline, improve the concentration of functional microorganism in the Waste Water Treatment, increase its retention time in biological processor.Adopt the immobilization technology of alginate calcium, polyvinyl alcohol (PVA), polyacrylamide embedding medium fixation of microbe cell such as (PAM) to be applied to the biological reinforced processing of dirt, waste water, as, the aerobic denitrifier that usefulness such as Patureau are embedded in the alginate ball carries out biological reinforced denitrogenation.Test-results shows that the aerobic denitrifier that is imbedded in the alginate ball has been improved aerobic denitrification process and function yeast retention time, has reduced the loss of thalline to a certain extent.But owing to exist: (1) embedding medium intensity and stability are not enough, the immobilized spherule fragmentation occurs after adding certain hour; (2) the embedding medium internal structure is closely knit, mass-transfer performance is poor, and the function yeast and the contacting of target contaminant that are embedded in the embedding medium are hindered, and microorganism lacks the space of growth and breeding; (3) problem such as immobilization cost height, so this technology also is difficult to use in dirty, waste water treatment engineering.
Summary of the invention
The technical problem to be solved in the present invention is to overcome deficiency of the prior art, utilizes microbial film to form the strong bacterium of ability and forms thicker microbial film at carrier surface, and aggegation altogether takes place and it is combined into microbial film with other bacterium.Strong film-forming bacterium is applied to sewage disposal to improve the ability of denitrogenation, reduces cost and streamlining management.
The present invention is achieved by the following technical measures.
Separation screening obtains strong film-forming bacterium from the microbial film of dirty, Waste Water Treatment and natural water body.
Separation screening and cultural method are:
The first step, the nutrient media components of strong film-forming bacterium
Nutrient media components: peptone 5 grams, yeast powder 2.5 grams, sodium-chlor 5 grams, water 1L, regulating the pH value is 7.0～7.4.Solid medium adds 15～20 gram agar.
Second step, the separation of bacterium on the microbial film:
Put into aseptic triangular flask with what gather with biomembranous carrier, add stroke-physiological saline solution, vibration is 20 minutes on vibrator, wash the bacterium on the microbial film, bacteria suspension is coated with not diluted and dilutes 10 and 100 times above-mentioned solid medium flat board behind serial dilution, cultivated 1～3 day for 30 ℃, it is standby that picking list bacterium colony from the various flat boards, the bacterial strain behind purifying are repeatedly put-80 ℃ and 4 ℃ of preservations.
The 3rd step, the screening of strong film-forming bacterium:
Be inoculated in above-mentioned solid plate respectively with separating all bacterial isolateses that obtain in second step, cultivate for 30 ℃ and carried out the bacterium activation in 24 hours, thalline after the picking activation identical liquid nutrient medium of transferring respectively again, cultivated 24 hours for 30 ℃, obtain bacterial suspension, in 1 milliliter the glass test tube of aforesaid liquid substratum is housed, inoculate 10 μ L bacterial suspension (OD respectively
600=1), 30 ℃, 100r/min shaking culture 24 hours, remove nutrient solution, the bacterium of not adsorbing with the deionized water flush away, repeat 2 times, adding 0.1% (W/V) Viola crystallina aqueous solution dyed 30 minutes for 2 milliliters, pour out staining fluid and wash 2 times, after the seasoning, add ethanol/acetone solution (80: 20V/V) 2 milliliters with deionized water, wash-out is adsorbed in the dyestuff on the microbial film, measure the light absorption value of ethanol/acetone solution,, therefrom filter out 3～5 the strongest strain bacteriums of film forming ability according to light absorption value size assessment biofilm biomass at the 570nm place.
The 4th step, the cultivation of strong film-forming bacterium:
Be inoculated in respectively in the 100 milliliters of liquid substratum separating the strong film-forming bacterium that obtains in the 3rd step, 30 ℃, 100r/min shaking culture 24 hours, the bacterial suspension that obtains is in the identical liquid nutrient medium of 1～10% (thalline weight in wet base/nutrient solution volume) ratio inoculation, spread cultivation step by step, finally obtain the cell concentration that needs.
Separation screening to strong film forming bacterium be Rhodopseudomonas or Aeromonas or Comamonas bacterium.
Separation screening to strong film forming bacterium be Rhodopseudomonas bacterium and Aeromonas bacteria combination or Aeromonas bacterium and Comamonas bacteria combination or Rhodopseudomonas bacterium and Comamonas bacteria combination.
Separation screening to strong film forming bacterium be Rhodopseudomonas bacterium, Aeromonas bacterium and Comamonas bacteria combination.
The method of utilizing strong film-forming bacterium to strengthen domestic sewage denitrifying is: in the sequencing batch biofilm sewage disposal device, sanitary sewage enters the sequencing batch biofilm sewage disposal device after septic tank is handled, in the Sewage treatment systems Start-up and Adjustment phase, add microbial film and form strong bacterium and nitrobacteria and the denitrifying bacterium of ability, total throwing bacterium amount (weight in wet base) is 0.1～2.0% of a sewage volume, the weight ratio that the types of functionality bacterium is dropped into is a strong film-forming bacterium: nitrated and nitrite bacteria: denitrifying bacterium=1～3: 2～5: 1～3, and can make sequencing batch biofilm Sewage treatment systems (at 2～3 months) in the long time keep higher nitric efficiency by adding of three kinds of function yeast.
After the present invention adopts abovementioned technology, that treatment technology and equipment is not complicated, keep on the basis of sequence batch (treatment system plurality of advantages, adding of strong film-forming bacterium makes the thicker microbial film of formation on the carrier, hinder dissolved oxygen and diffuse to microbial film inside, in the inner anaerobic environment that forms of microbial film, improved the nitric efficiency of Sewage treatment systems.Function yeast is fixed in microbial film, has reduced the number of times that adds of bacterium, has reduced cost, has also simplified management.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
The cultural method of strong film-forming bacterium is specific as follows:
The first step, the nutrient media components of strong film-forming bacterium
(1) nutrient media components: peptone 5 grams, yeast powder 2.5 grams, sodium-chlor 5 grams, water 1L, regulating the pH value is 7.0～7.4.Solid medium adds 15～20 gram agar.
Second step, the separation of bacterium on the microbial film:
Put into aseptic triangular flask with what gather with biomembranous carrier, add stroke-physiological saline solution, vibration is 20 minutes on vibrator, wash the bacterium on the microbial film, bacteria suspension is coated with not diluted and dilutes 10 and 100 times above-mentioned solid medium flat board behind serial dilution, cultivated 1～3 day for 30 ℃, it is standby that picking list bacterium colony from the various flat boards, the bacterial strain behind purifying are repeatedly put-80 ℃ and 4 ℃ of preservations.
The 3rd step, the screening of strong film-forming bacterium:
Be inoculated in above-mentioned solid plate respectively with separating all bacterial isolateses that obtain in second step, cultivated 24 hours for 30 ℃, the picking thalline identical liquid nutrient medium of transferring respectively again, cultivated 24 hours for 30 ℃, obtain bacterial suspension, in 1 milliliter the glass test tube of aforesaid liquid substratum is housed, inoculate 10 μ L bacterial suspensions (OD600=1) respectively, 30 ℃, 100r/min shaking culture 24 hours, remove nutrient solution,, repeat 2 times with the bacterium that the deionized water flush away does not adsorb, adding 0.1% (W/V) Viola crystallina aqueous solution dyed 30 minutes for 2 milliliters, pour out staining fluid and wash 2 times, after the seasoning, add ethanol/acetone solution (80: 20V/V) 2 milliliters with deionized water, wash-out is adsorbed in the dyestuff on the microbial film, measure the light absorption value of ethanol/acetone solution,, therefrom filter out 3～5 the strongest strain bacteriums of film forming ability according to light absorption value size assessment biofilm biomass at the 570nm place.
The 4th step, the cultivation of strong film-forming bacterium:
Be inoculated in respectively in the 100 milliliters of liquid substratum separating the strong film-forming bacterium that obtains in the 3rd step, 30 ℃, 100r/min shaking culture 24 hours, the bacterial suspension that obtains is in the identical liquid nutrient medium of 1～10% (thalline weight in wet base/nutrient solution volume) ratio inoculation, spread cultivation step by step, finally obtain the cell concentration that needs.
Embodiment 2:
2 useful volumes is the sewage that adds municipal sewage plant's preliminary sedimentation tank in the 10L biological contact oxidation reactor, adds porous spherical floating stuffing and the sheet glass carrier of being made by high-density polyethylene material in the reactor, and the filling ratio of filler is about 30%.To cultivate the strong film-forming bacterium that comes out, nitrobacteria, denitrifying bacterium three class bacterium among the embodiment 1 in No. 1 reactor by 1: 2: 1 mixed; In No. 2 reactors with nitrobacteria and denitrifying bacterium two class bacterium by 2: 1 mixed, the microorganism total amount (biomass weight in wet base) that adds in 2 reactors all is 1% of a sewage volume;
Reactor bottom is established aerating system fresh air is provided, vexed exposing to the sun 24 hours, beginning in second day is intake in the Continuous Flow mode, hydraulic detention time (HRT) 4～8 hours, operating temperature is 22～30 ℃, and through entering normal operating phase behind 7～14 days the operation limber up period, hydraulic detention time is 4 hours, dissolved oxygen content in the reactor sewage is at 0.5～2mg/L, and the treatment effect of 2 reactors is as follows:
Chemical oxygen demand (COD) (the COD of reactor water inlet
Cr) at 267.7～467.9mg/L, total nitrogen is at 26.3～49.7mg/L, reactor enters in behind the normal operating phase 20 days, and the COD clearance of No. 1 reactor is 69.5～82.9%, and nitrogen removal rate is 56.2～69.4%.No. 2 reactor COD clearances are 57.8～73.6%, and nitrogen removal rate is 33.2～57.4%.
Microbial film thickness on the suspension Ball-type packing in 2 reactors that add and do not add strong film-forming bacterium is measured, operation during 5d in No. 1 reactor the microbial film thickness on the carrier be 0.31～0.84 millimeter, the microbial film thickness in No. 2 reactors on the carrier is 0.13～0.46 millimeter.Operation during 30d in No. 1 reactor the microbial film thickness on the carrier be 1.71～2.84 millimeters, the microbial film thickness in No. 2 reactors on the carrier is 0.63～1.46 millimeter.
Embodiment 3:
Enter the sequencing batch biofilm reactor of 2 20L through the pretreated dispersion resident living of septic tank sewage, carrier is Sang Deqiu in the reactor, and Sang Deqiu makes the porous spherical shell by polypropylene, and inner filling plastic filler, the filling ratio of carrier are 30%.After the sequencing batch biofilm reactor water inlet, add by 1: 2: 1 mixed cultivating the strong film-forming bacterium, nitrobacteria, the denitrifying bacterium three class bacterium that come out among the embodiment 1 in No. 1 reactor; In No. 2 reactors nitrobacteria and denitrifying bacterium two class bacterium are added by 2: 1 mixed, the microorganism total amount (biomass weight in wet base) that adds in 2 reactors all is 1% of a sewage volume, simultaneously inoculate sanitary sewage disposal plant aeration tank active sludge in 2 reactors, inoculum size (mud weight in wet base) is 1% of a sewage volume.The operation scheme of reactor start-up limber up period is: aeration 4 hours, staticly settled 2 hours, and handled 24 hours in this circular treatment mode, press the cycle of operation operation that reactor is provided with then, 6 hours is a reaction time, wherein intakes aeration 4 hours 0.5 hour, precipitate 1 hour, water outlet was left standstill 0.5 hour, and rate of water exchange is 20%, treat stable water outlet after rate of water exchange transfer to 35%, the aeration phase dissolved oxygen concentration maintains 2.0～3.0mg/L, and temperature is 16～25 ℃.
The wastewater treatment efficiency of run duration is seen attached list.No. 1 reactor COD clearance is 51.5～89.9%, the NH4-N clearance is 46.2～81.0%, nitrogen removal rate is 48.4～76.4%, in the time of 49 days, still can keep higher nitric efficiency, No. 2 reactor COD clearances are 54.5～85.1%, the NH4-N clearance is 28.6～50.4%, nitrogen removal rate is 29.2～49.7%, nitric efficiency is close with No. 1 reactor in the time of the 7th day, nitric efficiency is lower than reactor all the time No. 1 after 14 days, and adding of strong film-forming bacterium makes treatment system keep comparatively permanent denitrification effect.
The subordinate list function yeast adds the wastewater treatment efficiency behind the sequencing batch biofilm reactor
Above-described only is preferred implementation of the present invention.Should be pointed out that for the person of ordinary skill of the art under the prerequisite that does not break away from the principle of the invention, can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Claims (5)
1. making of strong film-forming bacterium is characterized in that: separation screening obtains strong film-forming bacterium from the microbial film of dirty, Waste Water Treatment and natural water body.
Separation screening and cultural method are:
The first step, the nutrient media components of strong film-forming bacterium
Nutrient media components: peptone 5 grams, yeast powder 2.5 grams, sodium-chlor 5 grams, water 1L, regulating the pH value is 7.0～7.4.Solid medium adds 15～20 gram agar.
Second step, the separation of bacterium on the microbial film:
Put into aseptic triangular flask with what gather with biomembranous carrier, add stroke-physiological saline solution, vibration is 20 minutes on vibrator, wash the bacterium on the microbial film, bacteria suspension is coated with not diluted and dilutes 10 and 100 times above-mentioned solid medium flat board behind serial dilution, cultivated 1～3 day for 30 ℃, it is standby that picking list bacterium colony from the various flat boards, the bacterial strain behind purifying are repeatedly put-80 ℃ and 4 ℃ of preservations.
The 3rd step, the screening of strong film-forming bacterium:
Be inoculated in above-mentioned solid plate respectively with separating all bacterial isolateses that obtain in second step, cultivate for 30 ℃ and carried out the bacterium activation in 24 hours, the picking activatory thalline identical liquid nutrient medium of transferring respectively again, cultivated 24 hours for 30 ℃, obtain bacterial suspension, in 1 milliliter the glass test tube of aforesaid liquid substratum is housed, inoculate 10 μ L bacterial suspension (OD respectively
600=1), 30 ℃, 100r/min shaking culture 20～24 hours, remove nutrient solution, the bacterium of not adsorbing with the deionized water flush away, repeat 2 times, adding 0.1% (W/V) Viola crystallina aqueous solution dyed 30 minutes for 2 milliliters, pour out staining fluid and wash 2 times, after the seasoning, add ethanol/acetone solution (80: 20V/V) 2 milliliters with deionized water, wash-out is adsorbed in the dyestuff on the microbial film, measure the light absorption value of ethanol/acetone solution,, therefrom filter out 3～5 the strongest strain bacteriums of film forming ability according to light absorption value size assessment biofilm biomass at the 570nm place.
The 4th step, the cultivation of strong film-forming bacterium:
Be inoculated in respectively in the 100 milliliters of liquid substratum separating the strong film-forming bacterium that obtains in the 3rd step, 30 ℃, 100r/min shaking culture 24 hours, the bacterial suspension that obtains is in the identical liquid nutrient medium of 1～10% (thalline weight in wet base/nutrient solution volume) ratio inoculation, spread cultivation step by step, finally obtain the cell concentration that needs.
2. making of strong film-forming bacterium according to claim 1 is characterized in that: separation screening to strong film forming bacterium be Rhodopseudomonas bacterium or Aeromonas bacterium or Comamonas bacterium.
3. making of strong film-forming bacterium according to claim 1 is characterized in that: separation screening to strong film forming bacterium be Rhodopseudomonas bacterium and Aeromonas bacteria combination or Aeromonas bacterium and Comamonas bacteria combination or Rhodopseudomonas bacterium and Comamonas bacteria combination.
4. making of strong film-forming bacterium according to claim 1 is characterized in that: separation screening to strong film forming bacterium be Rhodopseudomonas bacterium, Aeromonas bacterium and Comamonas bacteria combination.
5. the method for strengthening sewage denitrification, it is characterized in that: in the sequencing batch biofilm sewage disposal device, in the Sewage treatment systems Start-up and Adjustment phase, add microbial film and form strong bacterium and nitrobacteria and the denitrifying bacterium of ability, total throwing bacterium amount (weight in wet base) is 0.1～2.0% (a thalline weight in wet base) of sewage volume, the weight ratio that the types of functionality bacterium is dropped into is a strong film-forming bacterium: nitrated and nitrite bacteria: denitrifying bacterium=1～3: 2～5: 1～3, and sanitary sewage can effective denitrogenation enter the sequence batch (treatment facility after septic tank is handled after.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100238758A CN101265458A (en) | 2008-04-21 | 2008-04-21 | Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100238758A CN101265458A (en) | 2008-04-21 | 2008-04-21 | Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101265458A true CN101265458A (en) | 2008-09-17 |
Family
ID=39988150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100238758A Pending CN101265458A (en) | 2008-04-21 | 2008-04-21 | Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101265458A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102079568A (en) * | 2010-07-23 | 2011-06-01 | 上海宜态科环保技术有限公司 | Online automatic microorganism dosing system |
| CN102616940A (en) * | 2012-01-18 | 2012-08-01 | 太原理工大学 | Purification treatment method for nickel plating wastewater |
| CN103215339A (en) * | 2013-02-06 | 2013-07-24 | 塔里木大学 | Escherichia coli biofilm detection method of 96-pore semi-quantitative method |
| CN103602723A (en) * | 2013-08-14 | 2014-02-26 | 塔里木大学 | Method for screening escherichia coli bio-membrane virulence genes |
| CN103723888A (en) * | 2013-12-14 | 2014-04-16 | 陈秀琼 | Technology for treating bleaching and dyeing waste water |
| CN106277365A (en) * | 2016-09-21 | 2017-01-04 | 湖北君集水处理有限公司 | A kind of Powdered Activated Carbon preparation method of granules with biodegradation and denitrification functions |
| CN109554309A (en) * | 2018-11-27 | 2019-04-02 | 北京凯得隆工贸有限公司 | One plant of comamonas W2 and its application in denitrogenation |
| CN112429839A (en) * | 2020-11-18 | 2021-03-02 | 复旦大学 | Enhanced SBBR (sequencing batch reactor) process for treating high-ammonia nitrogen sewage and wastewater |
| CN112591889A (en) * | 2020-12-28 | 2021-04-02 | 北京华晨吉光科技有限公司 | Denitrogenation method for microbial film reinforced film biological reactor |
| CN113698047A (en) * | 2021-09-23 | 2021-11-26 | 浙江省冶金研究院有限公司 | Method for purifying rural domestic sewage by microorganism-enhanced artificial wetland |
-
2008
- 2008-04-21 CN CNA2008100238758A patent/CN101265458A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102079568A (en) * | 2010-07-23 | 2011-06-01 | 上海宜态科环保技术有限公司 | Online automatic microorganism dosing system |
| CN102079568B (en) * | 2010-07-23 | 2012-12-05 | 宜态科环保技术(苏州)有限公司 | Online automatic microorganism dosing system |
| CN102616940A (en) * | 2012-01-18 | 2012-08-01 | 太原理工大学 | Purification treatment method for nickel plating wastewater |
| CN103215339A (en) * | 2013-02-06 | 2013-07-24 | 塔里木大学 | Escherichia coli biofilm detection method of 96-pore semi-quantitative method |
| CN103602723A (en) * | 2013-08-14 | 2014-02-26 | 塔里木大学 | Method for screening escherichia coli bio-membrane virulence genes |
| CN103723888B (en) * | 2013-12-14 | 2015-02-25 | 陈秀琼 | Technology for treating bleaching and dyeing waste water |
| CN103723888A (en) * | 2013-12-14 | 2014-04-16 | 陈秀琼 | Technology for treating bleaching and dyeing waste water |
| CN106277365A (en) * | 2016-09-21 | 2017-01-04 | 湖北君集水处理有限公司 | A kind of Powdered Activated Carbon preparation method of granules with biodegradation and denitrification functions |
| CN109554309A (en) * | 2018-11-27 | 2019-04-02 | 北京凯得隆工贸有限公司 | One plant of comamonas W2 and its application in denitrogenation |
| CN109554309B (en) * | 2018-11-27 | 2021-07-20 | 北京凯得隆工贸有限公司 | Comamonas W2 and application thereof in denitrification |
| CN112429839A (en) * | 2020-11-18 | 2021-03-02 | 复旦大学 | Enhanced SBBR (sequencing batch reactor) process for treating high-ammonia nitrogen sewage and wastewater |
| CN112591889A (en) * | 2020-12-28 | 2021-04-02 | 北京华晨吉光科技有限公司 | Denitrogenation method for microbial film reinforced film biological reactor |
| CN113698047A (en) * | 2021-09-23 | 2021-11-26 | 浙江省冶金研究院有限公司 | Method for purifying rural domestic sewage by microorganism-enhanced artificial wetland |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101265458A (en) | Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation | |
| Wang et al. | A review of biomass immobilization in anammox and partial nitrification/anammox systems: Advances, issues, and future perspectives | |
| CN101475362B (en) | Biochemical ceramic as well as preparation method and application thereof | |
| CN101544445B (en) | A method for restoring microorganism ecology of paper making waste water and a device | |
| CN100431982C (en) | Method of inhibiting membrane contamination of membrane bioreactor | |
| CN111876332A (en) | Preparation of algae biomembrane and application thereof in urban secondary effluent treatment | |
| CN103382049B (en) | Sequencing batch activated sludge reactor for sewage treatment and treatment process | |
| CN105236564A (en) | Combined packing for biological aerated filter and application of combined packing | |
| CN101338292A (en) | Method for preparing immobilization cold resistant strain | |
| CN108017234B (en) | Sewage treatment system and method | |
| CN102776140B (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| CN101624253A (en) | High efficient complex enzyme sewage treatment process and high efficient complex enzyme sewage treatment device | |
| CN1314604C (en) | Highland and severe cold areas sewage treatment method | |
| CN106957109B (en) | Horizontal flow biomembrane reaction device with functions of slowly releasing alkalinity and carbon source | |
| CN105174438A (en) | Improved MBR urban sewage treatment method | |
| CN106947711B (en) | A kind of preparation method of high applicability nitrification microbial inoculum | |
| Lin et al. | Recent advances in biofilm technologies for breeding wastewater treatment: Fundamentals, performance and impacts of antibiotics | |
| CN115108629B (en) | Advanced sewage treatment method and system | |
| CN114835255B (en) | Composite bioreactor based on iron-carbon carrier and preparation and sewage treatment method thereof | |
| CN116282553A (en) | Electroactive bacteria-algae biological film for enhancing pollutant degradation in pig raising wastewater, preparation method and application | |
| CN116354494A (en) | Composite slow-release carbon material coupled aerobic denitrifying bacteria and application thereof in water treatment | |
| CN206051664U (en) | A kind of utilization microorganism in situ excites the quick device for processing black and odorous water | |
| CN213895337U (en) | Device for improving denitrification-anaerobic ammonia oxidation coupling denitrification performance | |
| CN114314841A (en) | Immobilized biological filter biofilm formation method | |
| CN112520858B (en) | Method for improving biofilm formation efficiency and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20090109 Address after: Jiangsu 46, Jiangpu Road, Suzhou Industrial Zone, Suzhou, China: 215126 Applicant after: Suzhou Jiajing Water Treatment Equipment Co., Ltd. Address before: Room 406, block A, District 1, Su Xin building, 88 Jinji Lake Road, Suzhou Industrial Park, Jiangsu, Suzhou Province, China: 215008 Applicant before: Xuzhou Jialin Sci-Tech Development Co., Ltd. |
|
| ASS | Succession or assignment of patent right |
Owner name: SUZHOU JIAJING WATER TREATMENT EQUIPMENT CO., LTD. Free format text: FORMER OWNER: SUZHOU CITY JIALIN SCIENCE DEVELOPMENT CO., LTD. Effective date: 20090109 |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080917 |