CN103382049B - Sequencing batch activated sludge reactor for sewage treatment and treatment process - Google Patents

Abstract

The invention discloses a sequencing batch activated sludge reactor for sewage treatment and a treatment process, wherein the sequencing batch activated sludge reactor comprises a reactor main body, and an aeration device connected with an air pump is arranged in the reactor main body; the reactor main body is provided with a sample inlet and a water outlet, and the bottom of the reactor main body is provided with a sludge outlet; the aeration device comprises an aeration head arranged at the bottom of the reactor main body; the inner wall of the reactor body is rough. When the device is used for biological sewage treatment, the adding times of the strains are less, the durability is good, and activated sludge and pollutant degrading bacteria can form a film on the inner wall of the reactor with the help of a signal molecule preparation, so that various pollutants in sewage can be degraded quickly and effectively; the device has reliable principle, simple manufacturing structure, convenient monitoring and high treatment efficiency, and can be applied to actual industrial wastewater treatment after being amplified according to a certain proportion.

Description

A sequencing batch activated sludge reactor and treatment process for sewage treatment

technical field

The invention relates to the field of sewage treatment, in particular to a sequencing batch type activated sludge reactor and a treatment process for sewage treatment.

Background technique

Tobacco treatment wastewater and fluorine-containing organic wastewater are currently difficult industrial wastewater to treat. Biological methods are widely used in wastewater treatment because of their effectiveness, low cost, and the fact that biological methods can be adapted to continuously varying pollutant concentrations. The conventional activated sludge method is to use microorganisms to first convert organic matter into carbon dioxide, water and microbial cells, then preserve the microorganisms after the reaction, and remove the newly added microorganisms from the system by removing the remaining sludge at an appropriate time to achieve the goal of degrading pollution. Purpose.

Sequenced batch activated sludge process (SBR) is an activated sludge sewage treatment technology operated by intermittent aeration. In recent years, due to the simple structure, no secondary sedimentation tank, compact layout and less land occupation , more stable processing effect, flexible operation, easy to realize automation and other advantages, and more and more people pay attention to it. With the development of sewage treatment process, some new processes have appeared in SBR, and systems such as anaerobic SBR and multi-stage SBR have also been studied in depth. The invention patent with the notification number CN100369830C discloses a circulating sequencing batch type activated sludge sewage treatment process and its activated sludge reactor. The reactor is composed of two main reaction pools and two auxiliary reaction pools connected in series through a connecting channel or a control gate. Aeration devices are installed in the two main reaction pools, and the main reaction pool is arranged on the upstream wall of the two main reaction pools. The water inlet; the two auxiliary reaction tanks are equipped with a stirring device and a residual sludge pump, the upstream wall of the two auxiliary reaction tanks is arranged with the water inlet of the auxiliary reaction tank, and the downstream wall of the two auxiliary reaction tanks is arranged with a drainage device. The utility model patent with the publication number CN202369443U discloses an anaerobic, anaerobic and aerobic SBR reactor, which is in the shape of a cylindrical barrel as a whole, including a stirring wheel and paddle, driven by a stirring engine in the upper part, and an aeration port in the lower part of the reactor. Even with the blower, there are aeration pipes, sludge discharge pipes, water outlet pipes, sampling pipes, overflow pipes on the column wall of the reactor from bottom to top, and a water bath part for adjusting temperature outside the reaction part. In addition, there are exhaust ports, dissolved oxygen sensors, water inlet pipes, nitrate water inlet pipes, water inlet pumps, automatic time controllers, water distribution storage tanks, circuits and other parts. Most of these existing reactors that use aerobic bioaugmentation to treat wastewater have no carrier and smooth inner walls. Films are not easy to hang on the inner walls, and the activated sludge cannot be evenly distributed in the reactor. Many disadvantages.

The invention patent application with the publication number CN1772654A discloses a device and method for the suspension carrier SBR process to treat sewage and industrial wastewater in small towns and residential quarters. The suspension carrier SBR reactor is divided into a water inlet area, a main exposure area and an SBR area. The main exposure area is filled with suspended carriers, and the sewage treatment is carried out periodically. This method can increase the total amount of biological sludge and strengthen the activity of reactor sludge, but it has the disadvantages that the carrier cannot be reused and is easy to silt.

Contents of the invention

The invention provides a sequencing batch type activated sludge reactor for sewage treatment, which is used for rapidly and effectively degrading various pollutants in the sewage by a biological reinforcement method.

A sequencing batch type activated sludge reactor for sewage treatment, comprising a reactor main body, the reactor main body is provided with an aeration device connected with an air pump; the reactor main body is provided with a sample inlet and a drainage port , The bottom of the reactor main body is provided with a sludge outlet; the aeration device includes an aeration head arranged at the bottom of the reactor main body; the inner wall of the reactor main body is rough.

The inner wall of the main body of the reactor is rough, and it is easy to hang film, and the microorganisms and activated sludge can be evenly distributed in the reactor, thereby strengthening the biodegradation of sewage.

The reactor main body is cylindrical, and the ratio of height to inner diameter of the reactor main body is 8:1-10:1. Under the condition of this ratio, the aeration is the most favorable.

The sample inlet is arranged on the top of the reactor main body, which is convenient for sample injection.

The water outlet is arranged on the side wall of the reactor main body, which is convenient for water drainage or sampling.

The number of the drains is 2-7, and the drains are arranged on the side wall of the reactor body from bottom to top from the bottom of the reactor body, and the distance from the top drain to the top of the reactor body is 16-23% of height. Preferably, the number of the water outlets is five. The side wall of the reactor main body is provided with multiple outlets from bottom to top to facilitate sampling at different heights. For example, when aerating, mud-water mixed samples at different heights can be taken; when the gas is stopped, mud can be taken separately from bottom to top Samples, mixed samples and water samples for easy detection and monitoring. In addition, since foams are generated on the surface of the sewage during aeration treatment, when the distance between the uppermost outlet and the top of the reactor body accounts for 16-23% of the height of the reactor body, the foam will not overflow, and the utilization rate of equipment materials is the highest.

The sludge outlet is a round hole with a diameter of 0.8-1.0 cm, which is convenient for activated sludge to be discharged.

The inner wall of the reactor main body is provided with a number of granular protrusions, or is provided with rough textures, or is hung with nylon nets; wherein, the rough textures can be realized by roughening the inner wall. In this way, the inner wall can be conveniently set in a rough shape, and the microbial biofilm is easier to form on it, which is conducive to improving the degradation efficiency.

The aeration head is connected with an air pump outside the reactor main body through an aeration pipe, and the aeration rate of the air pump is 15-25 L/h.

The bottom of the reactor main body is provided with a fixing device, which plays a fixing role. The fixing device may be a fixing bracket.

Specifically, the reactor can adopt the following dimensions: the main body of the reactor is cylindrical, with a height of 62-67cm, an inner diameter of 7cm, and a wall thickness of 0.5-1.0cm; On the side wall, a drainage (mud) outlet is arranged at an interval of 13 cm from the bottom, and there are 5 outlets in total. The distance between the uppermost drainage outlet and the top of the reactor main body is 10-15 cm.

The present invention also provides a process for treating sewage by using the above sequencing batch activated sludge reactor, including: mixing activated sludge, sewage, pollutant-degrading bacteria seed liquid and signal molecule preparation, and adding them to the main body of the reactor; The aeration device performs aeration treatment on the mixed liquid, and after the gas is stopped, the sedimentation treatment is carried out to separate the mud and water; the treated sewage is discharged up to the standard.

The sewage can be tobacco waste water or organic fluorine waste water.

When the sewage is tobacco wastewater, the pollutant-degrading bacteria seed liquid is the seed liquid of Arthrobacter sp. TW with the preservation number CGMCC No. -Hexanoyl-homoserine lactone solution and N-hexanoyl-homoserine lactone solution with a concentration of 4-8mmol/L are mixed at a volume ratio of 2:1-1:2; the aeration treatment time is 18-48h , The precipitation treatment time is 5-15min.

Among them, Arthrobacter TW is classified as Arthrobacter sp. (Acinetobacter sp.), the strain number is TW, and has been preserved. The depository unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation date: November 2011, preservation number: CGMCC No. 7.47. The strain was also preserved in the Solid Waste Treatment and Resource Utilization Laboratory of Zhejiang Gongshang University. Wang et al. (Wang Meizhen, Yang Guiqin, Wang Xin, Yao Yanlai, Min Hang, Lv Zhenmei. Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp.TY and their responses in the presence of neonicotinoid insecticides. World Journal of Microbiology and Biotechnology, 2011, 27(7): 1633-1640.) disclose the identification of the bacterial strain. Arthrobacter TW is a Gram-negative bacterium, on the ISM solid medium containing nicotine or 4-fluoroaniline, the size is (0.5-0.7×1.6-2.0μm), and the colony is white and moist; In the ISM liquid medium, it is yellow-green, and in the ISM liquid medium containing 4-fluoroaniline, it is milky white. The strain 16S rDNA gene sequence (GenBank accession number is FJ753401) is shown in SEQ ID NO.1 in the sequence listing.

The N-3 oxo-hexanoyl-homoserine lactone solution is a mixture of N-3 oxo-hexanoyl-homoserine lactone dissolved in water and acetonitrile (v:v=1:1) The obtained solution, N-hexanoyl-homoserine lactone solution, is a solution obtained by dissolving N-hexanoyl-homoserine lactone in a mixed solution of water and acetonitrile (v:v=1:1). These two substances belong to N-acyl-homoserine lactones (AHLs) compounds, which are the most important type of signaling molecules in the quorum sensing system of Gram-negative bacteria, which regulate the expression of many physiological characteristics. The two solutions The signal molecule preparation mixed at a volume ratio of 2:1-1:2 can improve the properties of activated sludge to a certain extent, increase the secretion of extracellular polymers of Arthrobacter TW, promote the formation of biofilm, and facilitate the colonization of strains , so as to ensure the rapid degradation of the target pollutants.

In order to obtain a better synergistic effect, preferably, the volume ratio of the N-3 oxo-hexanoyl-homoserine lactone solution and the N-hexanoyl-homoserine lactone solution is 1:1.

Calculated per liter of tobacco wastewater, the addition amount of each substance is: 150-250mL of activated sludge, 10-30mL of Arthrobacter TW seed solution, and 5-10mL of signal molecule preparation. Under the condition of this dosage, the Arthrobacter TW seed solution and the signal molecule preparation are properly proportioned, which makes it easier for the strain to grow and reproduce in the activated sludge system, which is beneficial to the degradation of pollutants.

The Arthrobacter TW seed solution is prepared by the following method: insert Arthrobacter TW into a liquid medium, and culture it on a shaking table at 28-32°C for 18-28 hours; cultivate the strain by this culture method until the bacterial liquid OD600≥ 0.65, that is, the seed solution cultivated to the logarithmic growth phase. In terms of volume 1L, the liquid medium is composed of the following components: K ₂ HPO ₄ 0.15-0.25g, KH ₂ PO ₄ 0.7-0.9g, Na ₂ MoO ₄ ·H ₂ O 0.003-0.0036g, MgSO ₄ 0.15-0.25g, CaSO ₄ ·2H ₂ O 0.05-0.15g, FeSO ₄ ·7H ₂ O 0.003-0.007g, nicotine 0.8-1.2g, the balance is water; the pH of the liquid medium is 6-7. An appropriate amount of nicotine is added as a carbon and nitrogen source necessary for the growth and reproduction of Arthrobacter TW seed liquid.

When the sewage is organic fluorine wastewater, the seed liquid of pollutant-degrading bacteria is the seed liquid of Arthrobacter sp. TW with the preservation number CGMCC No.7.47, and the signal molecule preparation is composed of N-3 N-hexanoyl-homoserine lactone solution and N-hexanoyl-homoserine lactone solution with a concentration of 4-8mmol/L are mixed at a volume ratio of 2:1-1:2; the aeration treatment time is 24- 72h, precipitation treatment time is 5-15min.

Wherein, the volume ratio of N-3 oxo-hexanoyl-homoserine lactone solution and N-hexanoyl-homoserine lactone solution is 1:1.

The organic fluorine wastewater is wastewater containing 4-fluoroaniline. The 4-fluoroaniline-containing wastewater is the 4-fluoroaniline-containing wastewater that enters the aerobic stage after the drainage from organic fluorine processing plants is treated by anaerobic process in the early stage. For this wastewater, conventional methods are difficult to treat, but the combination of Arthrobacter TW and signal molecule preparation can selectively degrade 4-fluoroaniline with high degradation efficiency.

The concentration of 4-fluoroaniline in wastewater is 0.2-1.5g/L; the amount of each substance added per liter of wastewater is: activated sludge 150-250mL, Arthrobacter TW seed solution 20-40mL, signal molecule preparation 5- 10mL. Under the condition of this dosage, the Arthrobacter TW seed solution and the signal molecule preparation are properly proportioned, which makes it easier for the strain to grow and reproduce in the activated sludge system, which is beneficial to the degradation of pollutants.

The Arthrobacter TW seed solution is prepared by the following method: insert Arthrobacter TW into the liquid medium, and culture it on a shaking table at 28-32°C for 24-48h; cultivate the strain by this culture method until the bacterial liquid OD600≥ 0.65, that is, the seed solution cultivated to the logarithmic growth phase. In terms of volume 1L, the liquid medium is composed of the following components: K ₂ HPO ₄ 0.15-0.25g, KH ₂ PO ₄ 0.7-0.9g, Na ₂ MoO ₄ ·H ₂ O 0.003-0.0036g, MgSO ₄ 0.15-0.25g, CaSO ₄ 2H ₂ O 0.05-0.15g, FeSO ₄ 7H ₂ O 0.003-0.007g, 4-fluoroaniline 0.8-1.2g, the balance is water; the pH of the liquid medium is 7.5-8.5 . An appropriate amount of 4-fluoroaniline was added as a carbon and nitrogen source necessary for the growth and reproduction of Arthrobacter TW seed liquid.

The aeration device can be set as the following program: 5 minutes for sample injection, 5 minutes for drainage and sludge discharge, set 5-15 minutes of sedimentation treatment time according to the settlement performance of activated sludge, and set 18-72 hours of hydraulic retention time (aeration treatment time) according to the water output performance .

Adopt the inventive method, have following beneficial effect:

(1) The sequencing batch activated sludge reactor provided by the present invention has the advantages of reliable principle, simple manufacturing structure, convenient monitoring, and high processing efficiency, especially when used with signal molecule preparations, it has a very good effect and can be enlarged according to a certain ratio It can be applied to larger industrial wastewater treatment occasions.

(2) The inner wall of the reactor is rough. With the help of signal molecules, activated sludge and pollutant-degrading bacteria can hang film on the inner wall of the reactor. Under the same volume, the reaction efficiency is improved, which is an ideal high-efficiency , Economical and environmentally friendly new reactors for industrial sewage such as tobacco waste or organic fluorine wastewater, can reduce the harm of waste to the environment and human body, and are of great significance to environmental protection.

(3) The sewage treatment process provided by the present invention is easy to operate, has the advantages of less dosing times and long-lasting effect, can greatly shorten the start-up time of the reactor, and accelerate the stability of the reactor.

(4) By adopting the method of the present invention, pollutant-degrading bacteria and signal molecule preparations (which are almost non-toxic to microorganisms and can improve the properties of activated sludge) that are conducive to its colonization in the environment can be preferentially and rapidly activated under the condition of rich nutrient sources. , Efficiently degrade nicotine or 4-fluoroaniline; at the same time, when dealing with tobacco waste, it can also reduce the total COD value of tobacco waste.

Description of drawings

Fig. 1 is a structural schematic diagram of a sequencing batch activated sludge reactor (SBR) used for sewage treatment in the present invention.

Detailed ways

As shown in FIG. 1 , a sequencing batch activated sludge reactor for sewage treatment includes a reactor main body 1 and an aeration device 2 .

The reactor main body 1 is cylindrical, and the material is plexiglass; the height of the reactor main body 1 is 67 cm, the inner diameter is 7 cm, the wall thickness is 0.5 cm, and the effective volume is about 2 L.

The bottom of the reactor main body 1 is provided with a fixing device for fixing; the fixing device is a fixing bracket.

The top of the reactor main body 1 is provided with a sample inlet 11, which is used for adding sewage, activated sludge, etc.; the bottom of the reactor main body 1 is provided with a mud discharge port 13, and the mud discharge port 13 is a circular hole with a diameter of 0.8 cm. for the discharge of activated sludge.

The side wall of the reactor main body 1 is provided with 5 drainage outlets 12 along the vertical direction from bottom to top, and the lowest drainage outlet 12 is arranged at the bottom of the side wall of the reactor main body 1, and a drainage outlet is provided every 13 cm from the bottom. Port 12, the distance from the uppermost drain port 12 to the top of the reactor main body 1 is 15 cm.

A nylon net is hung on the inner wall of the reactor main body 1 to make the inner wall rough and easy to form a film. Microbes and activated sludge can be evenly distributed in the reactor, which can effectively improve the degradation efficiency.

The sample inlet 11, the mud discharge port 13, and the drain port 12 are connected with the external control system through pipelines, and regulating valves are arranged in the pipelines.

The aeration device 2 includes an aeration head 22 and an aeration pipe 21, the aeration head 22 is arranged at the bottom of the inner cavity of the reactor main body 1, and the aeration head 22 is connected with the air pump 3 outside the reactor main body 1 through the aeration pipe 21 .

Example 1 Adding Arthrobacter TW and signal molecule preparations that facilitate its degradation of pollutants to an SBR reactor with a rough inner wall to treat tobacco waste

(1) Screening and cultivation of Arthrobacter TW:

Tobacco waste from Hangzhou Liqun Cigarette Factory was taken and continuously cultured for 14 days to enrich nicotine-degrading bacteria; the enriched culture was diluted and inoculated on nicotine inorganic salt medium for culture; colonies were picked and continued to be diluted and cultured. until a single colony was picked.

After identification, the single colony was Arthrobacter sp., named Arthrobacter TW. Arthrobacter TW has been preserved. Deposit unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation date: October 2011, preservation number: CGMCC No.7.47. The strain is a Gram-negative bacterium with a size of (0.5-0.7×1.6-2.0 μm), and the colony of Arthrobacter TW is white and moist round; the GenBank accession number of the 16S rDNA sequence of the bacterium is FJ753401. Wang等(Wang Meizhen，Yang Guiqin，Wang Xin，Yao Yanlai，Min Hang，LvZhenmei.Nicotine degradation by two novel bacterial isolates ofAcinetobacter sp.TW and Sphingomonas sp.TY and their responses in thepresence of neonicotinoid insecticides.World Journal of Microbiology andBiotechnology , 2011, 27(7): 1633-1640.) disclosed the identification of Arthrobacter TW.

The formula of Arthrobacter TW seed liquid medium is: K ₂ HPO ₄ 0.2g/L, KH ₂ PO ₄ 0.8g/L, MgSO ₄ 0.2g/L, CaSO ₄ ·2H ₂ O0.1g/L, NaMoO ₄ · H ₂ O 0.0033g/L, FeSO ₄ ·7H ₂ O 0.005g/L, agar 10g/L (in solid medium), nicotine 1g/L, pH 6.5.

(2) Preparation of signal molecule preparations that are beneficial to nicotine degradation and bacterial strain film formation: the signal molecule preparations are composed of 3-oxo-C ₆ -HSL and C6-HSL, and the concentrations of the mother solutions are 8mmol/L and 4mmol/L, respectively, The mixing ratio of the two is 1:1, and the total volume is 10 mL. The function of the signal molecule preparation is to increase the secretion of extracellular polymers of bacterial strains, promote the formation of biofilms, and facilitate the colonization of bacterial strains, thereby ensuring the degradation of target pollutants.

(3) Disposal of tobacco waste:

Activated sludge (taken from Hangzhou Qige Sewage Treatment Plant), tobacco waste water (tobacco waste in tobacco waste water comes from Hangzhou Liqun Cigarette Factory, the concentration gradually increases with the operation of the reactor), Arthrobacter TW seed solution (cultivated to several growth stages) and the signal molecule preparation are mixed, and added into the reactor main body 1 of the SBR reactor from the injection port 11; the aeration device 2 is turned on to aerate the mixed solution, and after the gas is stopped, the sedimentation treatment is carried out to separate the mud and water; The treated sewage is discharged up to standard.

Among them, based on the tobacco wastewater per liter, the inoculum volume of activated sludge was 200mL; the inoculum volume of Arthrobacter TW seed culture solution was 20mL, and the addition amount of the signal molecule preparation beneficial to the degradation of nicotine was 10mL; the reaction conditions were room temperature, pH5. 0-8.0, DO is 3-5mg/L, aeration rate is 20L/h; the operation program is: 5min sample injection, 5min water drainage and sludge discharge, set the sludge settling time of 5-15min according to the settling performance of activated sludge , Set the hydraulic retention time of 18-48h according to the water outlet performance. The volume of reaction solution added after each drainage is 1/2 of the total volume of the reactor.

During aeration, samples can be taken separately through the five outlets 12 to obtain mixed samples of mud and water at different heights for detection; Used for detection and monitoring.

The treatment results show that Arthrobacter TW can be continuously monitored in the reactor sludge after inoculation once, and the activated sludge and strain TW are obviously hanging on the nylon mesh, showing the excellent effect of signal molecule preparations on biofilm formation , so that the activated sludge no longer only sinks at the bottom of the reactor, but is distributed throughout the reactor, so that the removal rate of nicotine and COD is maintained at a high level, and the continuous monitoring is 100d; after the reactor runs stably, the tobacco wastewater The degradation rate of nicotine within 24h HRT is 100%, and the nicotine influent concentration is between 1.0-1.5g/L; the COD removal rate exceeds 80%, and the COD concentration is about 25000mg/L after each water change in the reactor.

In Comparative Example 1, the SBR reactor with a rough inner wall in Example 1 was replaced with an SBR reactor with a smooth inner wall, that is, no nylon mesh was hung, and other operations were the same as the above examples.

The results show that Arthrobacter TW can be continuously monitored in the reactor sludge once inoculated once, and the continuous monitoring time is 100d; after the reactor runs stably, the degradation rate of nicotine in tobacco wastewater is 100% within 24hHRT, and nicotine enters The water concentration is between 1.0-1.5g/L; the COD removal rate is as high as 75%, and the COD concentration is about 25000mg/L after each water change in the reactor.

Example 2 Arthrobacter TW and signal molecule preparations that are beneficial to its degradation of pollutants are added to the SBR reactor with a rough inner wall to treat organic fluorine wastewater

(1) Culture of Arthrobacter TW:

Inoculate the Arthrobacter TW obtained by screening in Example 1 into an inorganic salt medium with a 4-fluoroaniline concentration of 1.0 g/L, and the medium formula is: K ₂ HPO ₄ 0.2 g/L, KH ₂ PO ₄ 0.8 g /L, MgSO ₄ 0.2g/L, CaSO ₄ ·2H ₂ O0.1g/L, NaMoO ₄ ·H ₂ O0.0033g/L, FeSO ₄ ·7H ₂ O0.005g/L, 4-fluoroaniline 1g/L , pH8.0; cultivated in a shaker at 130rpm and 30°C for 32h.

(2) The preparation of signal molecule preparations that are beneficial to the degradation of 4-fluoroaniline and the membrane formation of strains: the signal molecule preparations are composed of 3-oxo-C ₆ -HSL and C ₆ -HSL, and the concentrations of the mother solutions are 8mmol/L and 8mmol/L respectively. 4mmol/L, the mixing ratio of the two is 1:1, and the total volume is 10mL. The function of the signal molecule preparation is to increase the secretion of extracellular polymers of bacterial strains, promote the formation of biofilms, and facilitate the colonization of bacterial strains, thereby ensuring the degradation of target pollutants.

(3) Treatment of organic fluorine wastewater:

The activated sludge (taken from Hangzhou Qige Sewage Treatment Plant), organic fluorine wastewater (organic fluorine wastewater containing 4-fluoroaniline) is the aerobic stage produced by the organic fluorine processing plant drainage after the previous anaerobic treatment. Wastewater containing 4-fluoroaniline), Arthrobacter TW seed liquid (cultivated to the logarithmic growth phase) and signal molecule preparations are mixed, and added to the reactor body 1 of the SBR reactor from the inlet 11; the aeration device is turned on for 2 pairs of mixing The liquid is aerated, and after the gas is stopped, the sedimentation treatment is carried out to separate the mud and water; the treated sewage is discharged up to the standard.

Among them, based on the organic fluorine wastewater per liter, the inoculum volume of activated sludge is 200mL; the inoculum volume of Arthrobacter TW seed culture solution is 30mL, and the addition amount of signal molecule preparation that is beneficial to the degradation of nicotine is 10mL; the reaction conditions are room temperature, pH6 .5-9.5, DO is 3-5mg/L, aeration rate is 20L/h; the operation program is: 5min sample injection, 5min water drainage and sludge discharge, set 5-15min sludge sedimentation according to the settling performance of activated sludge , Set the hydraulic retention time of 24-72h according to the water outlet performance. The volume of reaction solution added after each drainage is 1/2 of the total volume of the reactor.

During aeration, samples can be taken separately through the five outlets 12 to obtain mixed samples of mud and water at different heights for detection; Used for detection and monitoring.

The treatment results show that Arthrobacter TW can be continuously monitored in the reactor sludge after inoculation once, and the activated sludge and strain TW are obviously hanging on the nylon mesh, showing the excellent effect of signal molecule preparations on biofilm formation In this way, the activated sludge no longer only sinks at the bottom of the reactor, but is distributed throughout the reactor, so that the removal rate of 4-fluoroaniline and COD is maintained at a high level, and the continuous monitoring is 100d; after the reactor is running stably, The degradation rate of 4-fluoroaniline within 32h HRT is 100%, and the influent concentration of 4-fluoroaniline is between 1.0-1.5g/L; the removal rate of COD exceeds 80%, and the COD concentration after each water change in the reactor Around 15000mg/L.

Comparative Example 2 In Example 2, the SBR reactor with a rough inner wall was replaced with an SBR reactor with a smooth inner wall, that is, no nylon mesh was hung, and other operations were the same as the above examples.

The results show that Arthrobacter TW can be continuously monitored in the reactor sludge with only one inoculation with 3% inoculation amount, and the continuous monitoring time is 100 days; after the reactor runs stably, 4-fluoro The degradation rate of aniline within 32h HRT is 100%, and the influent concentration of 4-fluoroaniline is between 1.0-1.5g/L; the removal rate of COD is 75%, and the COD concentration is 15000mg/L after each water change in the reactor. L or so.

Claims (6)

1. A sequence batch type activated sludge reactor for sewage treatment, comprising a reactor main body (1), the reactor main body (1) is provided with an aeration device (2) that links to each other with an air pump (3), It is characterized in that, the reactor main body (1) is provided with a sample inlet (11) and a drain outlet (12), and the bottom of the reactor main body (1) is provided with a sludge outlet (13); the aeration device ( 2) It includes an aeration head (22) arranged at the bottom of the reactor main body (1); the inner wall of the reactor main body (1) is provided with a number of granular protrusions or rough lines. 2. sequence batch type activated sludge reactor according to claim 1, is characterized in that, described reactor main body (1) is cylindrical, and the height of reactor main body (1) and internal diameter ratio are 8:1- 10:1. 3. The sequencing batch activated sludge reactor according to claim 1, characterized in that, the sample inlet (11) is arranged on the top of the reactor main body (1). 4. The sequencing batch activated sludge reactor according to claim 1, characterized in that, the water outlet (12) is arranged on the side wall of the reactor main body (1). 5. The sequencing batch type activated sludge reactor according to claim 1, characterized in that, the quantity of the drain outlet (12) is 2-7, and the drain outlet (12) is from the bottom of the reactor main body (1) It is arranged on the side wall of the reactor main body (1) from bottom to top, and the distance from the top drain port (12) to the top of the reactor main body (1) is 16-23% of the height of the reactor main body (1). 6. The sequencing batch activated sludge reactor according to claim 1, characterized in that, the sludge outlet (13) is a circular hole with a diameter of 0.8-1.0 cm.