CN102616940A - Purification treatment method for nickel plating wastewater - Google Patents
Purification treatment method for nickel plating wastewater Download PDFInfo
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Abstract
The present invention relates to a purification treatment method for nickel plating wastewater. According to the method, a microbiological method is adopted, nickel plating wastewater is simulated and prepared, microbial bacteria stenotrophomonas maltophilia is cultured, treatments of enrichment and purification are performed, then the bacteria is mixed with the nickel plating wastewater, an oscillation purification treatment is performed on heavy metals in the nickel plating wastewater, wherein the treatment purification efficiency is 98.5%, the reclaimed water standard in the national standard is reached, and the purified water can be used for washing and irrigation farmlands. The purification treatment method of the present invention has the following advantages that: the process is advanced and quick; the process is short; the data is abundant and accurate; the environmental pollution is not generated; the treatment purification efficiency on the nickel plating wastewater is high; the method is suitable for the industrial treatment, and is an ideal nickel plating wastewater purification treatment method; other wastewater containing heavy metals can further be treated and purified by the method of the present invention.
Description
Technical field
The present invention relates to a kind of purifying treatment method of nickel-plating waste water, belong to the technical field that heavy metal-containing waste water purifies and uses.
Background technology
Along with the development of industrial technology, the processing of metallic substance and surface-treated are used more and more frequent, the manufacturing of especially high-precision product; To metal products require increasingly high; For gain in strength on the metal products surface, hardness and erosion resistance, coating is carried out in the metallic surface of being everlasting, and also claims to electroplate; The metal of, good hardness corrosion-resistant with electrical effect plating one deck, for example nickel, chromium, zinc, copper, tin, vanadium etc. in the metallic surface; Galvanized medium is a water, and metal is soluble in water under electric field action, and under the condition of temperature field, metal anchors at the metallic surface uniformly, makes more satin of metallic surface, has also improved the hardness and the erosion resistance of metallic surface, is that the metal products surface-treated is first-selected.
Metal products will use and consume a large amount of clean waters in electroplating process; Should water do the electric field medium and carry out coating; Water repeatedly cleans again, in plating and cleaning process, will consume a large amount of clean waters, and in these water, can contain a large amount of heavy metals; For example nickel, zinc, copper, tin, vanadium, mercury, arsenic, beryllium, cadmium, lead, chromium etc.; To cause serious environmental to pollute after these water that contain heavy metal discharge, the discharging of these polluted water will cause soil and water source to go bad, and all can cause serious harm to human body and environment.
To sum up, contain heavy metal water processing, purify and to have become crucial research topic.
Summary of the invention
Goal of the invention
The objective of the invention is situation to background technology; With microbial method nickel-plating waste water is carried out purifying treatment; Adopt strain separating, bacterial strain purifying, strain culturing, purification of waste water, so that nickel-plating waste water is purified, with purification rate and the utilization ratio that increases substantially nickel-plating waste water.
Technical scheme
The chemical substance material that the present invention uses is: Carnis Bovis seu Bubali cream, peptone, sodium-chlor, sodium hydroxide, agar, nickelous chloride, dimethylglyoxime, iodine, potassiumiodide, EDTA Disodium, ammoniacal liquor, deionized water, it is following that it prepares consumption: with gram, milliliter is measure unit
Carnis Bovis seu Bubali cream: 20g ± 0.01g
Peptone: 40g ± 0.01g
Sodium-chlor: NaCl 20g ± 0.01g
Sodium hydroxide: NaOH 10g ± 0.01g
Agar: (C
12H
18O
9) n n=10-30 20g ± 0.01g
Nickelous chloride: NiCl
26H
2O 10g ± 0.01g
Dimethylglyoxime: C
4H
8N
2O
22g ± 0.01g
Iodine: I 15g ± 0.01g
Potassiumiodide: KI 30g ± 0.01g
EDTA Disodium: C
10H
14N
2O
8Na
22H
2O 50g ± 0.01g
Ammoniacal liquor: NH
350mL ± 0.1mL
Deionized water: H
2O 10000mL ± 10mL
Purifying treatment method is following:
(1) selected chemical substance material
The chemical substance material that purifying treatment is used will carry out selected, and carries out quality purity, content, concentration control:
Carnis Bovis seu Bubali cream: liquid paste nitrogen content >=13%
Peptone: solid-state solid nitrogen content >=12.5%
Sodium-chlor: solid-state solid 99%
Sodium hydroxide: solid-state solid 99%
Agar: solid-state solid 99%
Nickelous chloride: solid-state solid 99%
Dimethylglyoxime: solid-state solid 99%
Iodine: solid-state solid 99.8%
Potassiumiodide: solid-state solid 99%
EDTA Disodium: solid-state solid 99.9%
Ammoniacal liquor: liquid strength of fluid 27%
Deionized water: liquid liquid 99.99%
(2) preparation nickel-plating waste water
Take by weighing nickelous chloride 0.81g ± 0.01g, measure deionized water 2000mL ± 1mL, add in the beaker, place then on the electric mantle, stir 30min down for 55 ℃, become: nickel-plating waste water in electric field 220V, 50HZ, temperature;
(3) culturing micro-organisms bacterial classification: germ oligotrophy unit cell
1) preparation substratum
1. take by weighing Carnis Bovis seu Bubali cream 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g; Measure deionized water 1000mL ± 10mL; Add in first beaker, stir 10min, become: liquid substratum with whisking appliance; Liquid medium pH=7.3 are neutral;
2. take by weighing Carnis Bovis seu Bubali cream 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g; Measure deionized water 1000mL ± 10mL; Add in second beaker, stir 10min, become: nickeliferous liquid substratum with whisking appliance; Nickeliferous liquid medium pH=7.3 are neutral;
3. take by weighing Carnis Bovis seu Bubali cream 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the 3rd beaker; Whisking appliance stirs 5min, adds agar 15g ± 0.01g, on well heater, is heated to 97 ℃; Constant temperature insulation 5min dissolves agar, stirs with whisking appliance simultaneously; Become: the nickeliferous substratum of liquid paste, the nickeliferous medium pH of liquid paste=7.3 are neutral;
4. first beaker and liquid substratum, second beaker and liquid nickeliferous substratum, the 3rd beaker and the nickeliferous substratum of liquid paste are placed in the sterilization pressure cooker, the sterilization pressure cooker is placed on the electricradiator; In the sterilization pressure cooker, add deionized water 1000mL and sealing; Extract air in the sterilization pressure cooker, make the interior invariablenes pressure of liquid of sterilization pressure cooker at 0.14Mpa; Heat sterilization sterilization pressure cooker, sterilization pressure kettle temperature rises to 120 ℃ ± 2 ℃, and produces water vapor, steam sterilizing 25min ± 2min; Stop heating then, make it naturally cool to 25 ℃, take out three beakers, become respectively: liquid beef-protein medium, liquid nickeliferous beef-protein medium, solid-state nickeliferous beef-protein medium;
2) gather nickeliferous soil, the separating nickel tolerant bacteria
1. from the nickeliferous soil 10g of nickel plating collection in worksite, the ice chest of packing into is deposited 4 ℃ of temperature;
2. nickeliferous soil 10g is moved in the Erlenmeyer flask; Get liquid beef-protein medium 100mL and put into Erlenmeyer flask, Erlenmeyer flask is placed vibrate on the vibrator, vibration 2h under 30 ℃, oscillation frequency 150r/min; Leave standstill 10min, become: the deposition clear liquor;
3. get clear liquor 10mL, liquid nickeliferous beef-protein medium 200mL adds in the Erlenmeyer flask, cultivates 20h, one-tenth: bacterium liquid at 30 ℃, oscillation frequency 150r/min;
4. on the aseptic technique platform, get in bacterium liquid 1mL, the deionized water 1000mL adding beaker, mix, become: lower concentration bacterium liquid;
5. solid-state beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour petridish into, it is solidified; Add lower concentration bacterium liquid 0.1mL then, bacterium liquid is coated with full petridish, then petridish is placed incubator, under 30 ℃, leave standstill and cultivate 60h, and grow bacterium colony, that is: nickel tolerant bacteria bacterium colony with spreading rod;
3) nickel tolerant bacteria purifying
Solid-state nickeliferous beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour petridish into, it is solidified; Nickel tolerant bacteria bacterium colony is placed petridish, adopt strok method to carry out purifying, obtain single bacterium colony bacterium, that is: the nickel tolerant bacteria of purifying, the nickel tolerant bacteria is: germ oligotrophy unit cell;
4) cultivation of germ oligotrophy unit cell and enrichment
1. picking germ oligotrophy unit cell list bacterium colony inserts in the liquid beef-protein medium of 50mL, cultivates 20h, one-tenth: seed culture fluid down at 30 ℃, oscillation frequency 150r/min;
2. seed culture fluid is inserted liquid beef-protein medium in 1: 100 ratio, under 30 ℃, oscillation frequency 150r/min, cultivate 20h; The bacterium liquid of cultivating gets the bacterial precipitation thing at 4 ℃, 8000r/min spinning 10min;
3. the bacterial precipitation thing is placed quartzy product boat, places loft drier dry then, 55 ℃ of drying temperatures, time of drying 2h, bacterial powder, that is: germ oligotrophy unit cell powder;
(4) with germ oligotrophy unit cell purifying nickel-plating waste water
1) bacterial powder 0.5g, nickel-plating waste water 1000mL are added in the beaker, place the constant-temperature shaking culture case then, at 30 ℃, oscillation frequency 150r/min, duration of oscillation 4h;
2) at 4 ℃, 8000r/min spinning 5min; Collect supernatant, supernatant is purifies waste water;
(5) electromagnetism is softening purifies waste water
The electromagnetism of purifying waste water is softening to be accomplished in the electromagnetism groove, and will purify waste water places beaker, places electromagnetism groove and fixing then; Open the electromagnetism groove, electromagnetism groove magneticline of force intensity is 5000 Gausses, and electromagnetism time 60min purifies waste water for softening behind the electromagnetism;
(6) detect, chemically examine, analyze, characterize
Pattern, color and luster, chemical ingredients, chemical physics performance to purifying waste water detect, analyze;
With 16S rDNA sequence alignment method the bacterial isolates of purifying treatment is analyzed;
With the nickel ion concentration analysis of purifying waste water of dimethylglyoxime spectrophotometry;
Conclusion: purifying waste water is colourless transparent liquid, and purification rate is 98.5%, reaches middle water quality standard.
Beneficial effect
The present invention compares with background technology has tangible advance, is practical situation, employing microbial method, simulation preparation earlier nickel-plating waste water, culturing micro-organisms bacterial strain germ oligotrophy unit cell and the purification enrichment that contains heavy metal nickel to nickel-plating waste water, mixes vibration with nickel-plating waste water with bacterial strain; Heavy metal in the purifying treatment nickel-plating waste water, the treatment and purification rate is 98.5%, purifying waste water after the processing reached the middle water quality standard of GB regulation; Middle water can be used for washing, agricultural irrigation, and this purifying method technology is advanced, and is rapidly quick; Technical process is short, and informative data is accurate, and is free from environmental pollution; The purification rate of nickel-plating waste water is high; Can carry out industrial applications, be the purifying treatment method of very good nickel-plating waste water, other heavy metal-containing waste waters of this method treatment and purification also capable of using.
Description of drawings
Fig. 1 is preparation medium sterilization sterilized state figure
Fig. 2 is for cultivating the germ oligotrophy unit cell state graph
Fig. 3 is the state graph of purifying treatment nickel-plating waste water
Fig. 4 is the germ oligotrophy unit cell shape appearance figure
Fig. 5 is a germ oligotrophy unit cell to nickel ion adsorption rate variation diagram in time
Fig. 6 is a nickel tolerant bacteria strain identification route map
Shown in the figure, list of numerals is following:
1, sterilization pressure cooker, 2, pot cover, 3, electric controller, 4, image display, 5, PL, 6, power switch; 7, temperature regulator, 8, the pressure modulator, 9, valve tube, 10, vacuum pump, 11, tank; 12, escape pipe, 13, seal film, 14, sealing-ring, 15, valve, 16, rack; 17, first beaker, 18, liquid beef-protein medium, 19, second beaker, 20, liquid nickeliferous beef-protein medium, the 21, the 3rd beaker; 22, solid-state nickeliferous beef-protein medium, 23, the constant-temperature shaking culture case, 24, base, 25, thermal insulation layer, 26, vibrator; 27, spring support, 28, the incubator power switch, 29, the oscillation frequency push button, 30, the oscillation frequency image display, 31, the temperature push button; 32, temperature display, 33, Erlenmeyer flask, 34, inoculum, 35, container, 36, nickel-plating waste water+bacterial powder.
Embodiment
Below in conjunction with accompanying drawing the present invention is further specified:
Shown in Figure 1, be preparation medium sterilization sterilized state figure, it is correct that each location is wanted, according to quantity proportioning, operation according to the order of sequence.
The value of the chemical substance that preparation is used is to confirm by the scope that is provided with in advance, is measure unit with gram, milliliter.
The purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, and the preparation of bacteria culture medium is carried out in the sterilization pressure cooker, under 120 ℃ ± 2 ℃ of heating, pressure 0.14Mpa, accomplishes; Sterilization pressure cooker 1 is rectangular, and sterilization pressure cooker 1 places on the electric controller 3, and the top of sterilization pressure cooker 1 is pot cover 2 and by sealing-ring 14 sealing; Upper lateral part is escape pipe 12 and valve 15; At sterilization pressure cooker 1 inner bottom part is tank 11 and deionized water; On the top of tank 11 is rack 16; Put first beaker 17, second beaker 19, the 3rd beaker 21 side by side on the top of rack 16; It in first beaker 17 liquid beef-protein medium 18; In second beaker 19 be liquid nickeliferous beef-protein medium 20, the three beakers 21 interior be that solid-state nickeliferous beef-protein medium 22, the first beakers 17, second beaker 19, the 3rd beaker 21 tops are sealed by sealing film 13; The left side of sterilization pressure cooker 1 is provided with valve tube 9 and vacuum pump 10; On electric controller 3, be provided with image display 4, PL 5, power switch 6, temperature regulator 7, pressure modulator 8; Temperature in the sterilization pressure cooker 1 is 120 ℃ ± 2 ℃, and pressure values is 0.14Mpa.
Shown in Figure 2, for cultivating the germ oligotrophy unit cell state graph, it is correct that each location is wanted, according to quantity proportioning, operation according to the order of sequence.
The purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell; The cultivation of germ oligotrophy unit cell is carried out in the constant-temperature shaking culture case, and constant-temperature shaking culture case 23 is rectangular, and the bottom is provided with base 24; The casing of constant-temperature shaking culture case 23 is a thermal insulation layer 25; The mid-way is provided with vibrator 26 in casing, and vibrator 26 is provided with spring support 27, is Erlenmeyer flask 33 in the middle upper portion position of vibrator 26; Erlenmeyer flask 33 is fixing by spring support 27; The inside of Erlenmeyer flask 33 is inoculum 34, and seals by sealing film 13, and constant-temperature shaking culture case 23 is provided with power switch 28, oscillation frequency push button 29, oscillation frequency image display 30, temperature push button 31 and temperature display 32.
Shown in Figure 3, be the state graph of purifying treatment nickel-plating waste water, it is correct that each location is wanted, according to quantity proportioning, operation according to the order of sequence.
The purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell; In the constant-temperature shaking culture case, accomplish, constant-temperature shaking culture case 23 is rectangular, and the bottom is provided with base 24; The casing of constant-temperature shaking culture case 23 is a thermal insulation layer 25; The mid-way is a vibrator 26 in casing, on vibrator 26, is provided with spring support 27, is container 35 in the mid-way of vibrator 26; Container 35 is fixing by spring support 27; Container 35 inside are nickel-plating waste water+bacterial powder 36, and cover by sealing film 13, and constant-temperature shaking culture case 23 is provided with power switch 28, oscillation frequency push button 29, oscillation frequency image display 30, temperature push button 31 and temperature display 32.
Shown in Figure 4, be the germ oligotrophy unit cell shape appearance figure, can know among the figure: germ oligotrophy unit cell is shaft-like, long 0.5-2.3um, diameter≤0.6um.
Shown in Figure 5, for germ oligotrophy unit cell to nickel ion adsorption rate variation diagram in time, ordinate zou is that adsorption rate, X-coordinate are adsorption time, shows that germ oligotrophy unit cell is 98.5% to nickel ion maximum adsorption rate, absorption reaches balance time needs 4h; The chemical material that uses is dimethylglyoxime, iodine, potassiumiodide, EDTA Disodium, ammoniacal liquor, deionized water.
Shown in Figure 6, be nickel tolerant bacteria strain identification route map, the method for employing 16S rDNA gene sequencing is carried out the strain identification of nickel tolerant bacteria, and the concrete operations step is: 30 ℃, 150r/min vibration 20h cultivation nickel tolerant bacteria; Get bacterium liquid 5ml, 4 ℃, 12000r/min spinning 10min collection nickel tolerant bacteria; Extract test kit through genome and extract genomic dna; With the genomic dna is template, adds conservative primer 7f (5 '-CAGAGTTTGATCCTGGCT-3 ') and 1540r (1522) (5 '-AGGAGGTGATCCAGCCGCA-3 ') are carried out the PCR reaction 16SrDNA that increases; Carry out 16S rDNA sequencing through robotization dna sequencing appearance; With the 16S Microbial DB comparison of the state-run biotechnology of the 16S rDNA sequence and U.S. information center, can be known by comparison result: this nickel tolerant bacteria and germ oligotrophy unit cell homology are 99%, so this dientification of bacteria is a germ oligotrophy unit cell.
Claims (4)
1. the purifying treatment method of a nickel-plating waste water; It is characterized in that: the chemical substance material of use is: Carnis Bovis seu Bubali cream, peptone, sodium-chlor, sodium hydroxide, agar, nickelous chloride, dimethylglyoxime, iodine, potassiumiodide, EDTA Disodium, ammoniacal liquor, deionized water, it is following that it prepares consumption: with gram, milliliter is measure unit
Carnis Bovis seu Bubali cream: 20g ± 0.01g
Peptone: 40g ± 0.01g
Sodium-chlor: NaCl 20g ± 0.01g
Sodium hydroxide: NaOH 10g ± 0.01g
Agar: (C
12H
18O
9) n n=10-30 20g ± 0.01g
Nickelous chloride: NiCl
26H
2O 10g ± 0.01g
Dimethylglyoxime: C
4H
8N
2O
22g ± 0.01g
Iodine: I 15g ± 0.01g
Potassiumiodide: KI 30g ± 0.01g
EDTA Disodium: C
10H
14N
2O
8Na
22H
2O 50g ± 0.01g
Ammoniacal liquor: NH
350mL ± 0.1mL
Deionized water: H
2O 10000mL ± 10mL
Purifying treatment method is following:
(1) selected chemical substance material
The chemical substance material that purifying treatment is used will carry out selected, and carries out quality purity, content, concentration control:
Carnis Bovis seu Bubali cream: liquid paste nitrogen content >=13%
Peptone: solid-state solid nitrogen content >=12.5%
Sodium-chlor: solid-state solid 99%
Sodium hydroxide: solid-state solid 99%
Agar: solid-state solid 99%
Nickelous chloride: solid-state solid 99%
Dimethylglyoxime: solid-state solid 99%
Iodine: solid-state solid 99.8%
Potassiumiodide: solid-state solid 99%
EDTA Disodium: solid-state solid 99.9%
Ammoniacal liquor: liquid strength of fluid 27%
Deionized water: liquid liquid 99.99%
(2) preparation nickel-plating waste water
Take by weighing nickelous chloride 0.81g ± 0.01g, measure deionized water 2000mL ± 1mL, add in the beaker, place then on the electric mantle, stir 30min down for 55 ℃, become: nickel-plating waste water in electric field 220V, 50HZ, temperature;
(3) culturing micro-organisms bacterial classification: germ oligotrophy unit cell
1) preparation substratum
1. take by weighing Carnis Bovis seu Bubali cream 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g; Measure deionized water 1000mL ± 10mL; Add in first beaker, stir 10min, become: liquid substratum with whisking appliance; Liquid medium pH=7.3 are neutral;
2. take by weighing Carnis Bovis seu Bubali cream 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g; Measure deionized water 1000mL ± 10mL; Add in second beaker, stir 10min, become: nickeliferous liquid substratum with whisking appliance; Nickeliferous liquid medium pH=7.3 are neutral;
3. take by weighing Carnis Bovis seu Bubali cream 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the 3rd beaker; Whisking appliance stirs 5min, adds agar 15g ± 0.01g, on well heater, is heated to 97 ℃; Constant temperature insulation 5min dissolves agar, stirs with whisking appliance simultaneously; Become: the nickeliferous substratum of liquid paste, the nickeliferous medium pH of liquid paste=7.3 are neutral;
4. first beaker and liquid substratum, second beaker and liquid nickeliferous substratum, the 3rd beaker and the nickeliferous substratum of liquid paste are placed in the sterilization pressure cooker, the sterilization pressure cooker is placed on the electricradiator; In the sterilization pressure cooker, add deionized water 1000mL and sealing; Extract air in the sterilization pressure cooker, make the interior invariablenes pressure of liquid of sterilization pressure cooker at 0.14Mpa; Heat sterilization sterilization pressure cooker, sterilization pressure kettle temperature rises to 120 ℃ ± 2 ℃, and produces water vapor, steam sterilizing 25min ± 2min; Stop heating then, make it naturally cool to 25 ℃, take out three beakers, become respectively: liquid beef-protein medium, liquid nickeliferous beef-protein medium, solid-state nickeliferous beef-protein medium;
2) gather nickeliferous soil, the separating nickel tolerant bacteria
1. from the nickeliferous soil 10g of nickel plating collection in worksite, the ice chest of packing into is deposited 4 ℃ of temperature;
2. nickeliferous soil 10g is moved in the Erlenmeyer flask; Get liquid beef-protein medium 100mL and put into Erlenmeyer flask, Erlenmeyer flask is placed vibrate on the vibrator, vibration 2h under 30 ℃, oscillation frequency 150r/min; Leave standstill 10min, become: the deposition clear liquor;
3. get clear liquor 10mL, liquid nickeliferous beef-protein medium 200mL adds in the Erlenmeyer flask, cultivates 20h, one-tenth: bacterium liquid at 30 ℃, oscillation frequency 150r/min;
4. on the aseptic technique platform, get in bacterium liquid 1mL, the deionized water 1000mL adding beaker, mix, become: lower concentration bacterium liquid;
5. solid-state beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour petridish into, it is solidified; Add lower concentration bacterium liquid 0.1mL then, bacterium liquid is coated with full petridish, then petridish is placed incubator, under 30 ℃, leave standstill and cultivate 60h, and grow bacterium colony, that is: nickel tolerant bacteria bacterium colony with spreading rod;
3) nickel tolerant bacteria purifying
Solid-state nickeliferous beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour petridish into, it is solidified; Nickel tolerant bacteria bacterium colony is placed petridish, adopt strok method to carry out purifying, obtain single bacterium colony bacterium, that is: the nickel tolerant bacteria of purifying, the nickel tolerant bacteria is: germ oligotrophy unit cell;
4) cultivation of germ oligotrophy unit cell and enrichment
1. picking germ oligotrophy unit cell list bacterium colony inserts in the liquid beef-protein medium of 50mL, cultivates 20h, one-tenth: seed culture fluid down at 30 ℃, oscillation frequency 150r/min;
2. seed culture fluid is inserted liquid beef-protein medium in 1: 100 ratio, under 30 ℃, oscillation frequency 150r/min, cultivate 20h; The bacterium liquid of cultivating gets the bacterial precipitation thing at 4 ℃, 8000r/min spinning 10min;
3. the bacterial precipitation thing is placed quartzy product boat, places loft drier dry then, 55 ℃ of drying temperatures, time of drying 2h, bacterial powder, that is: germ oligotrophy unit cell powder;
(4) with germ oligotrophy unit cell purifying nickel-plating waste water
1) bacterial powder 0.5g, nickel-plating waste water 1000mL are added in the beaker, place the constant-temperature shaking culture case then, at 30 ℃, oscillation frequency 150r/min, duration of oscillation 4h;
2) at 4 ℃, 8000r/min spinning 5min; Collect supernatant, supernatant is purifies waste water;
(5) electromagnetism is softening purifies waste water
The electromagnetism of purifying waste water is softening to be accomplished in the electromagnetism groove, and will purify waste water places beaker, places electromagnetism groove and fixing then; Open the electromagnetism groove, electromagnetism groove magneticline of force intensity is 5000 Gausses, and electromagnetism time 60min purifies waste water for softening behind the electromagnetism;
(6) detect, chemically examine, analyze, characterize
Pattern, color and luster, chemical ingredients, chemical physics performance to purifying waste water detect, analyze;
With 16S rDNA sequence alignment method the bacterial isolates of purifying treatment is analyzed;
With the nickel ion concentration analysis of purifying waste water of dimethylglyoxime spectrophotometry;
Conclusion: purifying waste water is colourless transparent liquid, and purification rate is 98.5%, reaches middle water quality standard.
2. the purifying treatment method of a kind of nickel-plating waste water according to claim 1; It is characterized in that: the purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell; The preparation of bacteria culture medium is carried out in the sterilization pressure cooker, under 120 ℃ ± 2 ℃ of heating, pressure 0.14Mpa, accomplishes; Sterilization pressure cooker (1) is rectangular, and sterilization pressure cooker (1) places on the electric controller (3), and the top of sterilization pressure cooker (1) is pot cover (2) and is sealed by sealing-ring (14); Upper lateral part is escape pipe (12) and valve (15); At sterilization pressure cooker (1) inner bottom part is tank (11) and deionized water; On the top of tank (11) is rack (16); Put first beaker (17), second beaker (19), the 3rd beaker (21) side by side on the top of rack (16); In first beaker (17) liquid beef-protein medium (18); In second beaker (19) is liquid nickeliferous beef-protein medium (20), is solid-state nickeliferous beef-protein medium (22) in the 3rd beaker (21), and seal by sealing film (13) on first beaker (17), second beaker (19), the 3rd beaker (21) top; The left side of sterilization pressure cooker (1) is provided with valve tube (9) and vacuum pump (10); On electric controller (3), be provided with image display (4), PL (5), power switch (6), temperature regulator (7), pressure modulator (8); Temperature in the sterilization pressure cooker (1) is 120 ℃ ± 2 ℃, and pressure values is 0.14Mpa.
3. the purifying treatment method of a kind of nickel-plating waste water according to claim 1; It is characterized in that: the purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell; The cultivation of germ oligotrophy unit cell is carried out in the constant-temperature shaking culture case, and constant-temperature shaking culture case (23) is rectangular, and the bottom is provided with base (24); The casing of constant-temperature shaking culture case (23) is thermal insulation layer (25); The mid-way is provided with vibrator (26) in casing, and vibrator (26) is provided with spring support (27), is Erlenmeyer flask (33) in the middle upper portion position of vibrator (26); Erlenmeyer flask (33) is fixing by spring support (27); The inside of Erlenmeyer flask (33) is inoculum (34), and seals by sealing film (13), and constant-temperature shaking culture case (23) is provided with power switch (28), oscillation frequency push button (29), oscillation frequency image display (30), temperature push button (31) and temperature display (32).
4. the purifying treatment method of a kind of nickel-plating waste water according to claim 1, it is characterized in that: the purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, in the constant-temperature shaking culture case, accomplishes; Constant-temperature shaking culture case (23) is rectangular; The bottom is provided with base (24), and the casing of constant-temperature shaking culture case (23) is thermal insulation layer (25), and the mid-way is vibrator (26) in casing; On vibrator (26), be provided with spring support (27); In the mid-way of vibrator (26) is container (35), and container (35) is fixing by spring support (27), and container (35) inside is nickel-plating waste water+bacterial powder (36); And seal by sealing film (13), constant-temperature shaking culture case (23) is provided with power switch (28), oscillation frequency push button (29), oscillation frequency image display (30), temperature push button (31) and temperature display (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210025789 CN102616940B (en) | 2012-01-18 | 2012-01-18 | Purification treatment method for nickel plating wastewater |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434446A (en) * | 2016-09-23 | 2017-02-22 | 北京林业大学 | Anti-antimony bacteria NXH3 and application thereof |
| CN112322550A (en) * | 2020-11-25 | 2021-02-05 | 湖北环境修复与治理技术研究有限公司 | Stenotrophomonas maltophilia and application thereof in heavy metal treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101265458A (en) * | 2008-04-21 | 2008-09-17 | 苏州市嘉林科技发展有限公司 | Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation |
| CN101479200A (en) * | 2006-07-06 | 2009-07-08 | 株式会社日立工业设备技术 | Inclusive immobilization support, wastewater disposal apparatus and wastewater disposal method using the inclusive immobilization support |
| JP2011056383A (en) * | 2009-09-09 | 2011-03-24 | Japan Organo Co Ltd | Treatment method of nitrogen containing water and treatment apparatus of nitrogen containing water |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101479200A (en) * | 2006-07-06 | 2009-07-08 | 株式会社日立工业设备技术 | Inclusive immobilization support, wastewater disposal apparatus and wastewater disposal method using the inclusive immobilization support |
| CN101265458A (en) * | 2008-04-21 | 2008-09-17 | 苏州市嘉林科技发展有限公司 | Method for preparing strong film-forming bacterium and reinforcing sewage denitrogenation |
| JP2011056383A (en) * | 2009-09-09 | 2011-03-24 | Japan Organo Co Ltd | Treatment method of nitrogen containing water and treatment apparatus of nitrogen containing water |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434446A (en) * | 2016-09-23 | 2017-02-22 | 北京林业大学 | Anti-antimony bacteria NXH3 and application thereof |
| CN106434446B (en) * | 2016-09-23 | 2019-09-17 | 北京林业大学 | One plant of anti-antimony bacterium NXH3 and its application |
| CN112322550A (en) * | 2020-11-25 | 2021-02-05 | 湖北环境修复与治理技术研究有限公司 | Stenotrophomonas maltophilia and application thereof in heavy metal treatment |
| CN112322550B (en) * | 2020-11-25 | 2022-09-20 | 湖北环境修复与治理技术研究有限公司 | Stenotrophomonas maltophilia and application thereof in heavy metal treatment |
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