CN102616940B - Purification treatment method for nickel plating wastewater - Google Patents
Purification treatment method for nickel plating wastewater Download PDFInfo
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Abstract
The present invention relates to a purification treatment method for nickel plating wastewater. According to the method, a microbiological method is adopted, nickel plating wastewater is simulated and prepared, microbial bacteria stenotrophomonas maltophilia is cultured, treatments of enrichment and purification are performed, then the bacteria is mixed with the nickel plating wastewater, an oscillation purification treatment is performed on heavy metals in the nickel plating wastewater, wherein the treatment purification efficiency is 98.5%, the reclaimed water standard in the national standard is reached, and the purified water can be used for washing and irrigation farmlands. The purification treatment method of the present invention has the following advantages that: the process is advanced and quick; the process is short; the data is abundant and accurate; the environmental pollution is not generated; the treatment purification efficiency on the nickel plating wastewater is high; the method is suitable for the industrial treatment, and is an ideal nickel plating wastewater purification treatment method; other wastewater containing heavy metals can further be treated and purified by the method of the present invention.
Description
Technical field
The present invention relates to a kind of purifying treatment method of nickel-plating waste water, belong to the technical field that heavy metal-containing waste water purifies and uses.
Background technology
Development along with industrial technology, the processing of metallic substance and surface-treated are used more and more frequent, the manufacturing of especially high-precision product, requirement to metal products is more and more higher, for gain in strength on the metal products surface, hardness and erosion resistance, coating is carried out in the metallic surface of being everlasting, and also claims to electroplate, the metal corrosion-resistant with electrical effect plating one deck in the metallic surface, that hardness is good, such as nickel, chromium, zinc, copper, tin, vanadium etc.; The medium of electroplating is water, and metal is soluble in water under electric field action, and under the condition of temperature field, metal anchors at the metallic surface uniformly, makes more satin of metallic surface, has also improved hardness and the erosion resistance of metallic surface, is that the metal products surface-treated is first-selected.
Metal products will use and consume a large amount of clean waters in electroplating process, should water do the electric field medium and carry out coating, water repeatedly cleans again, in plating and cleaning process, will consume a large amount of clean waters, and in these water, can contain a large amount of heavy metals, such as nickel, zinc, copper, tin, vanadium, mercury, arsenic, beryllium, cadmium, lead, chromium etc., after discharging, these water that contain heavy metal will cause serious environmental pollution, the discharging of these polluted water will cause soil and water source to go bad, and all can cause serious harm to human body and environment.
To sum up, contain heavy metal water processing, purify and to have become very important research topic.
Summary of the invention
Goal of the invention
The objective of the invention is the situation for background technology, with microbial method nickel-plating waste water is carried out purifying treatment, adopt strain separating, bacterial strain purifying, strain culturing, purification of waste water, so that nickel-plating waste water is purified, with purification rate and the utilization ratio that increases substantially nickel-plating waste water.
Technical scheme
The chemical substance material that the present invention uses is: extractum carnis, peptone, sodium-chlor, sodium hydroxide, agar, nickelous chloride, dimethylglyoxime, iodine, potassiumiodide, disodium ethylene diamine tetraacetate, ammoniacal liquor, deionized water, it is as follows that it prepares consumption: take gram, milliliter as measure unit
Extractum carnis: 20g ± 0.01g
Peptone: 40g ± 0.01g
Sodium-chlor: NaCl 20g ± 0.01g
Sodium hydroxide: NaOH 10g ± 0.01g
Agar: (C
12H
18O
9) n n=10-30 20g ± 0.01g
Nickelous chloride: NiCl
26H
2O 10g ± 0.01g
Dimethylglyoxime: C
4H
8N
2O
22g ± 0.01g
Iodine: I 15g ± 0.01g
Potassiumiodide: KI 30g ± 0.01g
Disodium ethylene diamine tetraacetate: C
10H
14N
2O
8Na
22H
2O 50g ± 0.01g
Ammoniacal liquor: NH
350mL ± 0.1mL
Deionized water: H
2O 10000mL ± 10mL
Purifying treatment method is as follows:
(1) selected chemical substance material
The chemical substance material that purifying treatment is used will carry out selected, and carries out quality purity, content, concentration control:
Extractum carnis: liquid paste nitrogen content 〉=13%
Peptone: solid-state solid nitrogen content 〉=12.5%
Sodium-chlor: solid-state solid 99%
Sodium hydroxide: solid-state solid 99%
Agar: solid-state solid 99%
Nickelous chloride: solid-state solid 99%
Dimethylglyoxime: solid-state solid 99%
Iodine: solid-state solid 99.8%
Potassiumiodide: solid-state solid 99%
Disodium ethylene diamine tetraacetate: solid-state solid 99.9%
Ammoniacal liquor: liquid strength of fluid 27%
Deionized water: liquid liquid 99.99%
(2) preparation nickel-plating waste water
Take by weighing nickelous chloride 0.81g ± 0.01g, measure deionized water 2000mL ± 1mL, add in the beaker, then place on the electric mantle, at electric field 220V, 50HZ, 55 ℃ of lower 30min that stir of temperature, become: nickel-plating waste water;
(3) culturing micro-organisms bacterial classification: germ oligotrophy unit cell
1) preparation substratum
1. take by weighing extractum carnis 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the first beaker, stir 10min with agitator, become: liquid culture medium, liquid culture medium pH=7.3 is neutral;
2. take by weighing extractum carnis 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the second beaker, stir 10min with agitator, become: nickeliferous liquid culture medium, nickeliferous liquid culture medium pH=7.3 is neutral;
3. take by weighing extractum carnis 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the 3rd beaker, agitator stirs 5min, add agar 15g ± 0.01g, be heated to 97 ℃ at well heater, constant temperature insulation 5min dissolves agar, stir with agitator simultaneously, become: the nickeliferous substratum of liquid paste, the nickeliferous medium pH of liquid paste=7.3 are neutral;
4. the first beaker and liquid culture medium, the second beaker and liquid nickeliferous substratum, the 3rd beaker and the nickeliferous substratum of liquid paste are placed in the sterilization pressure cooker, the sterilization pressure cooker is placed on the electricradiator; In the sterilization pressure cooker, add deionized water 1000mL and sealing; Extract air in the sterilization pressure cooker, make the interior invariablenes pressure of liquid of sterilization pressure cooker at 0.14Mpa; Heat sterilization sterilization pressure cooker, sterilization pressure kettle temperature rises to 120 ℃ ± 2 ℃, and produces water vapor, steam sterilizing 25min ± 2min; Then stopped heating makes it naturally cool to 25 ℃, takes out three beakers, becomes respectively: liquid beef-protein medium, liquid nickeliferous beef-protein medium, solid-state nickeliferous beef-protein medium;
2) gather nickeliferous soil, the separating nickel tolerant bacteria
1. from the nickeliferous soil 10g of nickel plating collection in worksite, the ice chest of packing into is deposited 4 ℃ of temperature;
2. nickeliferous soil 10g is moved in the Erlenmeyer flask, get liquid beef-protein medium 100mL and put into Erlenmeyer flask, Erlenmeyer flask is placed vibrate on the vibrator, 2h vibrates under 30 ℃, oscillation frequency 150r/min, leave standstill 10min, become: the precipitation clear liquor;
3. get clear liquor 10mL, liquid nickeliferous beef-protein medium 200mL and add in the Erlenmeyer flask, cultivate 20h, one-tenth: bacterium liquid at 30 ℃, oscillation frequency 150r/min;
4. on aseptic operating platform, get bacterium liquid 1mL, deionized water 1000mL and add in the beaker, mix, become: lower concentration bacterium liquid;
5. solid-state beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour culture dish into, it is solidified; Then add lower concentration bacterium liquid 0.1mL, with spreading rod bacterium liquid is coated with full culture dish, then culture dish is placed incubator, under 30 ℃, leave standstill and cultivate 60h, and grow bacterium colony, that is: nickel tolerant bacteria bacterium colony;
3) nickel tolerant bacteria purifying
Solid-state nickeliferous beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour culture dish into, it is solidified; Nickel tolerant bacteria bacterium colony is placed culture dish, adopt method of scoring to carry out purifying, obtain single bacterium colony bacterium, that is: the nickel tolerant bacteria of purifying, the nickel tolerant bacteria is: germ oligotrophy unit cell;
4) cultivation of germ oligotrophy unit cell and enrichment
1. picking germ oligotrophy unit cell list bacterium colony in the liquid beef-protein medium of access 50mL, is cultivated 20h under 30 ℃, oscillation frequency 150r/min, become: seed culture fluid;
2. seed culture fluid is accessed liquid beef-protein medium in 1: 100 ratio, under 30 ℃, oscillation frequency 150r/min, cultivate 20h; The bacterium liquid of cultivating gets the bacterial precipitation thing at 4 ℃, 8000r/min centrifugation 10min;
3. the bacterial precipitation thing is placed quartzy product boat, then place loft drier dry, 55 ℃ of drying temperatures, time of drying, 2h got bacterial powder, that is: the germ oligotrophy unit cell powder;
(4) with germ oligotrophy unit cell purifying nickel-plating waste water
1) bacterial powder 0.5g, nickel-plating waste water 1000mL are added in the beaker, then place constant-temperature shaking incubator, at 30 ℃, oscillation frequency 150r/min, duration of oscillation 4h;
2) at 4 ℃, 8000r/min centrifugation 5min; Collect supernatant liquor, supernatant liquor is purifies waste water;
(5) electromagnetism is softening purifies waste water
The electromagnetism of purifying waste water is softening to be finished in the electromagnetism groove, and will purify waste water places beaker, then places electromagnetism groove and fixing; Open the electromagnetism groove, electromagnetism groove magnetic line of force intensity is 5000 Gausses, and electromagnetism time 60min purifies waste water for softening behind the electromagnetism;
(6) detect, chemically examine, analyze, characterize
Pattern, color and luster, chemical composition, the chemical physics performance of purifying waste water detected, analyzes;
With 16S rDNA sequence alignment method the bacterial isolates of purifying treatment is analyzed;
With the nickel ion concentration analysis of purifying waste water of dimethylglyoxime spectrophotometry;
Conclusion: purifying waste water is colourless transparent liquid, and purification rate is 98.5%, reaches middle water quality standard.
Beneficial effect
The present invention compares with background technology has obvious advance, the practical situation that contain heavy metal nickel for nickel-plating waste water, adopt microbial method, first simulation preparation nickel-plating waste water, culturing micro-organisms bacterial strain germ oligotrophy unit cell and purification enrichment, mix vibration with nickel-plating waste water with bacterial strain, heavy metal in the purifying treatment nickel-plating waste water, the treatment and purification rate is 98.5%, purifying waste water after the processing reached the middle water quality standard of GB regulation, middle water can be used for washing, agricultural irrigation, this purifying method technique is advanced, rapidly quick, technical process is short, and informative data is accurate, and is free from environmental pollution, the purification rate of nickel-plating waste water is high, can carry out industrial applications, be the purifying treatment method of very good nickel-plating waste water, also can utilize other heavy metal-containing waste waters of this method treatment and purification.
Description of drawings
Fig. 1 is preparation medium sterilization sterilized state figure
Fig. 2 is for cultivating the germ oligotrophy unit cell state graph
Fig. 3 is the state graph of purifying treatment nickel-plating waste water
Fig. 4 is the germ oligotrophy unit cell shape appearance figure
Fig. 5 is that germ oligotrophy unit cell is to nickel ion adsorption rate temporal evolution figure
Fig. 6 is nickel tolerant bacteria strain identification route map
Shown in the figure, list of numerals is as follows:
1, the sterilization pressure cooker, 2, pot cover, 3, electric controller, 4, display screen, 5, pilot lamp, 6, power switch, 7, temperature regulator, 8, the pressure modulator, 9, valve tube, 10, vacuum pump, 11, tank, 12, escape pipe, 13, sealed membrane, 14, sealing-ring, 15, control valve, 16, rack, 17, the first beaker, 18, liquid beef-protein medium, 19, the second beaker, 20, liquid nickeliferous beef-protein medium, 21, the 3rd beaker, 22, solid-state nickeliferous beef-protein medium, 23, constant-temperature shaking incubator, 24, base, 25, thermal insulation layer, 26, vibrator, 27, spring support, 28, the incubator power switch, 29, the oscillation frequency push button, 30, the oscillation frequency display screen, 31, the temperature push button, 32, temperature display, 33, Erlenmeyer flask, 34, inoculum, 35, container, 36, nickel-plating waste water+bacterial powder.
Embodiment
The present invention will be further described below in conjunction with accompanying drawing:
Shown in Figure 1, be preparation medium sterilization sterilized state figure, it is correct that want each position, according to quantity proportioning, according to the order of sequence operation.
The value of the chemical substance that preparation is used is to determine by the scope that sets in advance, take gram, milliliter as measure unit.
The purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, and the preparation of bacteria culture medium is carried out in the sterilization pressure cooker, finishes under 120 ℃ ± 2 ℃ of heating, pressure 0.14Mpa; Sterilization pressure cooker 1 is rectangular, and sterilization pressure cooker 1 places on the electric controller 3, and the top of sterilization pressure cooker 1 is pot cover 2 and by sealing-ring 14 sealing; Upper lateral part is escape pipe 12 and control valve 15; Be tank 11 and deionized water at sterilization pressure cooker 1 inner bottom part, be rack 16 on the top of tank 11, put side by side the first beaker 17, the second beaker 19, the 3rd beaker 21 on the top of rack 16, it is liquid beef-protein medium 18 in the first beaker 17, be liquid nickeliferous beef-protein medium 20 in the second beaker 19, be that solid-state nickeliferous beef-protein medium 22, the first beakers 17, the second beaker 19, the 3rd beaker 21 tops seal by sealed membrane 13 in the 3rd beaker 21; The left side of sterilization pressure cooker 1 is provided with valve tube 9 and vacuum pump 10; Be provided with display screen 4, pilot lamp 5, power switch 6, temperature regulator 7, pressure modulator 8 at electric controller 3; Temperature in the sterilization pressure cooker 1 is 120 ℃ ± 2 ℃, and pressure values is 0.14Mpa.
Shown in Figure 2, for cultivating the germ oligotrophy unit cell state graph, it is correct that want each position, according to quantity proportioning, according to the order of sequence operation.
The purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, the cultivation of germ oligotrophy unit cell is carried out in constant-temperature shaking incubator, constant-temperature shaking incubator 23 is rectangular, the bottom is provided with base 24, the casing of constant-temperature shaking incubator 23 is thermal insulation layer 25, the mid-way is provided with vibrator 26 in casing, vibrator 26 is provided with spring support 27, be Erlenmeyer flask 33 in the middle upper portion position of vibrator 26, Erlenmeyer flask 33 is fixing by spring support 27, the inside of Erlenmeyer flask 33 is inoculum 34, and sealed by sealed membrane 13, constant-temperature shaking incubator 23 is provided with power switch 28, oscillation frequency push button 29, oscillation frequency display screen 30, temperature push button 31 and temperature display 32.
Shown in Figure 3, be the state graph of purifying treatment nickel-plating waste water, it is correct that want each position, according to quantity proportioning, according to the order of sequence operation.
The purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, in constant-temperature shaking incubator, finish, constant-temperature shaking incubator 23 is rectangular, the bottom is provided with base 24, the casing of constant-temperature shaking incubator 23 is thermal insulation layer 25, the mid-way is vibrator 26 in casing, be provided with spring support 27 at vibrator 26, be container 35 in the mid-way of vibrator 26, container 35 is fixing by spring support 27, container 35 inside are nickel-plating waste water+bacterial powder 36, and covered by sealed membrane 13, constant-temperature shaking incubator 23 is provided with power switch 28, oscillation frequency push button 29, oscillation frequency display screen 30, temperature push button 31 and temperature display 32.
Shown in Figure 4, be the germ oligotrophy unit cell shape appearance figure, among the figure as can be known: germ oligotrophy unit cell is shaft-like, long 0.5-2.3um, diameter≤0.6um.
Shown in Figure 5, for germ oligotrophy unit cell to nickel ion adsorption rate temporal evolution figure, ordinate zou is that adsorption rate, X-coordinate are adsorption time, shows that germ oligotrophy unit cell is 98.5% to nickel ion maximum adsorption rate, the time that absorption reaches balance needs 4h; The chemical material that uses is dimethylglyoxime, iodine, potassiumiodide, disodium ethylene diamine tetraacetate, ammoniacal liquor, deionized water.
Shown in Figure 6, be nickel tolerant bacteria strain identification route map, the method for employing 16S rDNA gene sequencing is carried out the strain identification of nickel tolerant bacteria, and concrete operation step is: 30 ℃, 150r/min vibration 20h cultivation nickel tolerant bacteria; Get bacterium liquid 5ml, 4 ℃, 12000r/min centrifugation 10min collection nickel tolerant bacteria; Extract test kit by genome and extract genomic dna; Take genomic dna as template, add conservative primer pair 7f (5 '-CAGAGTTTGATCCTGGCT-3 ') and 1540r (1522) (5 '-AGGAGGTGATCCAGCCGCA-3 ') and carry out the PCR reaction 16SrDNA that increases; Carry out 16S rDNA sequencing by the automatization DNA sequencer; The 16S Microbial database of the state-run biotechnology of 16S rDNA sequence and U.S. information center is compared, and by comparison result as can be known: this nickel tolerant bacteria and germ oligotrophy unit cell homology are 99%, therefore this dientification of bacteria is germ oligotrophy unit cell.
Claims (4)
1. the purifying treatment method of a nickel-plating waste water, it is characterized in that: the chemical substance material of use is: extractum carnis, peptone, sodium-chlor, sodium hydroxide, agar, nickelous chloride, dimethylglyoxime, iodine, potassiumiodide, disodium ethylene diamine tetraacetate, ammoniacal liquor, deionized water, it is as follows that it prepares consumption: take gram, milliliter as measure unit
Extractum carnis: 20g ± 0.01g
Peptone: 40g ± 0.01g
Sodium-chlor: NaCl 20g ± 0.01g
Sodium hydroxide: NaOH 10g ± 0.01g
Agar: (C
12H
18O
9) n n=10-30 20g ± 0.01g
Nickelous chloride: NiCl
26H
2O 10g ± 0.01g
Dimethylglyoxime: C
4H
8N
2O
22g ± 0.01g
Iodine: I 15g ± 0.01g
Potassiumiodide: KI 30g ± 0.01g
Disodium ethylene diamine tetraacetate: C
10H
14N
2O
8Na
22H
2O 50g ± 0.01g
Ammoniacal liquor: NH
350mL ± 0.1mL
Deionized water: H
2O 10000mL ± 10mL
Purifying treatment method is as follows:
(1) selected chemical substance material
The chemical substance material that purifying treatment is used will carry out selected, and carries out quality purity, content, concentration control:
Extractum carnis: liquid paste nitrogen content 〉=13%
Peptone: solid-state solid nitrogen content 〉=12.5%
Sodium-chlor: solid-state solid 99%
Sodium hydroxide: solid-state solid 99%
Agar: solid-state solid 99%
Nickelous chloride: solid-state solid 99%
Dimethylglyoxime: solid-state solid 99%
Iodine: solid-state solid 99.8%
Potassiumiodide: solid-state solid 99%
Disodium ethylene diamine tetraacetate: solid-state solid 99.9%
Ammoniacal liquor: liquid strength of fluid 27%
Deionized water: liquid liquid 99.99%
(2) preparation nickel-plating waste water
Take by weighing nickelous chloride 0.81g ± 0.01g, measure deionized water 2000mL ± 1mL, add in the beaker, then place on the electric mantle, at electric field 220V, 50HZ, 55 ℃ of lower 30min that stir of temperature, become: nickel-plating waste water;
(3) culturing micro-organisms bacterial classification: germ oligotrophy unit cell
1) preparation substratum
1. take by weighing extractum carnis 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the first beaker, stir 10min with agitator, become: liquid culture medium, liquid culture medium pH=7.3 is neutral;
2. take by weighing extractum carnis 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the second beaker, stir 10min with agitator, become: nickeliferous liquid culture medium, nickeliferous liquid culture medium pH=7.3 is neutral;
3. take by weighing extractum carnis 3g ± 0.01g, peptone 10g ± 0.01g, sodium-chlor 5g ± 0.01g, nickelous chloride 2.377g ± 0.01g, sodium hydroxide 0.001g ± 0.0001g, measure deionized water 1000mL ± 10mL, add in the 3rd beaker, agitator stirs 5min, add agar 15g ± 0.01g, be heated to 97 ℃ at well heater, constant temperature insulation 5min dissolves agar, stir with agitator simultaneously, become: the nickeliferous substratum of liquid paste, the nickeliferous medium pH of liquid paste=7.3 are neutral;
4. the first beaker and liquid culture medium, the second beaker and liquid nickeliferous substratum, the 3rd beaker and the nickeliferous substratum of liquid paste are placed in the sterilization pressure cooker, the sterilization pressure cooker is placed on the electricradiator; In the sterilization pressure cooker, add deionized water 1000mL and sealing; Extract air in the sterilization pressure cooker, make the interior invariablenes pressure of liquid of sterilization pressure cooker at 0.14Mpa; Heat sterilization sterilization pressure cooker, sterilization pressure kettle temperature rises to 120 ℃ ± 2 ℃, and produces water vapor, steam sterilizing 25min ± 2min; Then stopped heating makes it naturally cool to 25 ℃, takes out three beakers, becomes respectively: liquid beef-protein medium, liquid nickeliferous beef-protein medium, solid-state nickeliferous beef-protein medium;
2) gather nickeliferous soil, the separating nickel tolerant bacteria
1. from the nickeliferous soil 10g of nickel plating collection in worksite, the ice chest of packing into is deposited 4 ℃ of temperature;
2. nickeliferous soil 10g is moved in the Erlenmeyer flask, get liquid beef-protein medium 100mL and put into Erlenmeyer flask, Erlenmeyer flask is placed vibrate on the vibrator, 2h vibrates under 30 ℃, oscillation frequency 150r/min, leave standstill 10min, become: the precipitation clear liquor;
3. get clear liquor 10mL, liquid nickeliferous beef-protein medium 200 mL and add in the Erlenmeyer flask, cultivate 20h, one-tenth: bacterium liquid at 30 ℃, oscillation frequency 150r/min;
4. on aseptic operating platform, get bacterium liquid 1mL, deionized water 1000 mL and add in the beaker, mix, become: lower concentration bacterium liquid;
5. solid-state beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour culture dish into, it is solidified; Then add lower concentration bacterium liquid 0.1mL, with spreading rod bacterium liquid is coated with full culture dish, then culture dish is placed incubator, under 30 ℃, leave standstill and cultivate 60h, and grow bacterium colony, that is: nickel tolerant bacteria bacterium colony;
3) nickel tolerant bacteria purifying
Solid-state nickeliferous beef-protein medium is heated to 97 ℃ ± 2 ℃, stirs 5min, become paste, get 20mL and pour culture dish into, it is solidified; Nickel tolerant bacteria bacterium colony is placed culture dish, adopt method of scoring to carry out purifying, obtain single bacterium colony bacterium, that is: the nickel tolerant bacteria of purifying, the nickel tolerant bacteria is: germ oligotrophy unit cell;
4) cultivation of germ oligotrophy unit cell and enrichment
1. picking germ oligotrophy unit cell list bacterium colony in the liquid beef-protein medium of access 50mL, is cultivated 20h under 30 ℃, oscillation frequency 150r/min, become: seed culture fluid;
2. seed culture fluid is accessed liquid beef-protein medium in the ratio of 1:100, under 30 ℃, oscillation frequency 150r/min, cultivate 20h; The bacterium liquid of cultivating gets the bacterial precipitation thing at 4 ℃, 8000r/min centrifugation 10min;
3. the bacterial precipitation thing is placed quartzy product boat, then place loft drier dry, 55 ℃ of drying temperatures, time of drying, 2h got bacterial powder, that is: the germ oligotrophy unit cell powder;
(4) with germ oligotrophy unit cell purifying nickel-plating waste water
1) bacterial powder 0.5g, nickel-plating waste water 1000mL are added in the beaker, then place constant-temperature shaking incubator, at 30 ℃, oscillation frequency 150r/min, duration of oscillation 4h;
2) at 4 ℃, 8000r/min centrifugation 5min; Collect supernatant liquor, supernatant liquor is purifies waste water;
(5) electromagnetism is softening purifies waste water
The electromagnetism of purifying waste water is softening to be finished in the electromagnetism groove, and will purify waste water places beaker, then places electromagnetism groove and fixing; Open the electromagnetism groove, electromagnetism groove magnetic line of force intensity is 5000 Gausses, and electromagnetism time 60min purifies waste water for softening behind the electromagnetism;
(6) detect, chemically examine, analyze, characterize
Pattern, color and luster, chemical composition, the chemical physics performance of purifying waste water detected, analyzes;
With 16S rDNA sequence alignment method the bacterial isolates of purifying treatment is analyzed;
With the nickel ion concentration analysis of purifying waste water of dimethylglyoxime spectrophotometry;
Conclusion: purifying waste water is colourless transparent liquid, and purification rate is 98.5%, reaches middle water quality standard.
2. the purifying treatment method of a kind of nickel-plating waste water according to claim 1, it is characterized in that: the purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, the preparation of bacteria culture medium is carried out in the sterilization pressure cooker, finishes under 120 ℃ ± 2 ℃ of heating, pressure 0.14Mpa; Sterilization pressure cooker (1) is rectangular, and sterilization pressure cooker (1) places on the electric controller (3), and the top of sterilization pressure cooker (1) is pot cover (2) and is sealed by sealing-ring (14); Upper lateral part is escape pipe (12) and control valve (15); Be tank (11) and deionized water at sterilization pressure cooker (1) inner bottom part, be rack (16) on the top of tank (11), put side by side the first beaker (17) on the top of rack (16), the second beaker (19), the 3rd beaker (21), be liquid beef-protein medium (18) in the first beaker (17), be liquid nickeliferous beef-protein medium (20) in the second beaker (19), be solid-state nickeliferous beef-protein medium (22) in the 3rd beaker (21), the first beaker (17), the second beaker (19), the 3rd beaker (21) top seals by sealed membrane (13); The left side of sterilization pressure cooker (1) is provided with valve tube (9) and vacuum pump (10); Be provided with display screen (4), pilot lamp (5), power switch (6), temperature regulator (7), pressure modulator (8) at electric controller (3).
3. the purifying treatment method of a kind of nickel-plating waste water according to claim 1, it is characterized in that: the purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, the cultivation of germ oligotrophy unit cell is carried out in constant-temperature shaking incubator, constant-temperature shaking incubator (23) is rectangular, the bottom is provided with base (24), the casing of constant-temperature shaking incubator (23) is thermal insulation layer (25), the mid-way is provided with vibrator (26) in casing, vibrator (26) is provided with spring support (27), be Erlenmeyer flask (33) in the middle upper portion position of vibrator (26), Erlenmeyer flask (33) is fixing by spring support (27), the inside of Erlenmeyer flask (33) is inoculum (34), and sealed by sealed membrane (13), constant-temperature shaking incubator (23) is provided with power switch (28), oscillation frequency push button (29), oscillation frequency display screen (30), temperature push button (31) and temperature display (32).
4. the purifying treatment method of a kind of nickel-plating waste water according to claim 1, it is characterized in that: the purifying treatment of nickel-plating waste water is carried out with the bacterium germ oligotrophy unit cell, in constant-temperature shaking incubator, finish, constant-temperature shaking incubator (23) is rectangular, the bottom is provided with base (24), the casing of constant-temperature shaking incubator (23) is thermal insulation layer (25), the mid-way is vibrator (26) in casing, be provided with spring support (27) at vibrator (26), be container (35) in the mid-way of vibrator (26), container (35) is fixing by spring support (27), container (35) inside is nickel-plating waste water+bacterial powder (36), and sealed by sealed membrane (13), constant-temperature shaking incubator (23) is provided with power switch (28), oscillation frequency push button (29), oscillation frequency display screen (30), temperature push button (31) and temperature display (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210025789 CN102616940B (en) | 2012-01-18 | 2012-01-18 | Purification treatment method for nickel plating wastewater |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210025789 CN102616940B (en) | 2012-01-18 | 2012-01-18 | Purification treatment method for nickel plating wastewater |
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