CN110079487A - Bacterium and its application of one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion - Google Patents
Bacterium and its application of one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion Download PDFInfo
- Publication number
- CN110079487A CN110079487A CN201910476990.9A CN201910476990A CN110079487A CN 110079487 A CN110079487 A CN 110079487A CN 201910476990 A CN201910476990 A CN 201910476990A CN 110079487 A CN110079487 A CN 110079487A
- Authority
- CN
- China
- Prior art keywords
- ion
- bacillus thuringiensis
- bacterium
- plants
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 241000894006 Bacteria Species 0.000 title claims abstract description 32
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 30
- 229910001430 chromium ion Inorganic materials 0.000 title claims abstract description 13
- 230000009467 reduction Effects 0.000 title claims abstract description 12
- 238000001556 precipitation Methods 0.000 title claims abstract description 10
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 48
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 48
- 239000001963 growth medium Substances 0.000 claims abstract description 33
- 239000002609 medium Substances 0.000 claims description 17
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 239000011651 chromium Substances 0.000 claims description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 9
- 229910052804 chromium Inorganic materials 0.000 claims description 9
- 238000012549 training Methods 0.000 claims description 3
- 241000726221 Gemma Species 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 23
- 239000011133 lead Substances 0.000 description 20
- 239000007788 liquid Substances 0.000 description 12
- -1 Plumbum ion Chemical class 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 8
- 238000002955 isolation Methods 0.000 description 8
- 108020004465 16S ribosomal RNA Proteins 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000012010 growth Effects 0.000 description 5
- 229910021645 metal ion Inorganic materials 0.000 description 5
- 230000005855 radiation Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000002441 X-ray diffraction Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- BFGKITSFLPAWGI-UHFFFAOYSA-N chromium(3+) Chemical compound [Cr+3] BFGKITSFLPAWGI-UHFFFAOYSA-N 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000006137 Luria-Bertani broth Substances 0.000 description 2
- 208000031320 Teratogenesis Diseases 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 230000033558 biomineral tissue development Effects 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001603 reducing effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028400 Mutagenic effect Diseases 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000005260 alpha ray Effects 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 231100000429 cutaneous necrosis Toxicity 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007799 dermal corrosion Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229910000156 lead(II) phosphate Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004995 male reproductive system Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000243 mutagenic effect Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000009894 physiological stress Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 229910052820 pyromorphite Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 231100000108 skin corrosion Toxicity 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/075—Bacillus thuringiensis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides bacterium and its application of one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion, belongs to field of biotechnology.The division bacteria be named as bacillus thuringiensis (Bacillus thuringiensis) HM-311 plants, deposit number are as follows: GDMCC NO:60592.The HM-311 plants of abilities with mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion, can be used for Heavy Metal Pollution Control.The bacterial strain is 2100 mg/L to the highest tolerable concentration of lead ion, and the highest tolerable concentration to hexavalent chromium is 600 mg/L.Fermented and cultured two days in the culture medium containing 1500mg/L lead ion can remove in culture medium 97% lead ion；Containing 100 mg/LCr6+Culture medium in fermented and cultured two days, in culture medium 70% hexavalent chromium ion can be restored.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium from
The bacterium of son and its application.
Background technique
Unreasonable exploitation with the industrial expansions such as dyestuff, plating and people to metal mineral reserves, more and more weights
Metal enters water body.Heavy metal has teratogenesis, carcinogenic, mutagenesis, since heavy metal is not easy to be degraded by organism, and can lead to
It crosses food chain to transmit and be enriched with, human body can be entered through number of ways, seriously endanger human health.In China, lead and chromium are two kinds
The heavy metal of common pollution of ecological environment.
Lead has the characteristics that permeability is strong, harmless treatment is difficult as strong toxic heavy metal, is to cause food pollution most
One of serious heavy metal contaminants.Human body mainly passes through diet and the big approach intake lead of breathing two, and accumulation is arrived certain in body
After limitation, a series of system injury such as brain, kidney, liver, nerve, bone can be caused, while also there is certain teratogenesis, cause
Cancer, mutagenic effect.Lead in food is mainly derived from natural environment release and artificial pollution.Natural environment release includes mine
Area's rock weathering and submarine volcanic eruption etc. can be attached to the media such as ambient air, soil and water body with natural subsidence effect,
And then pass through the adsorption and osmosis contaminated food products raw material of plant, influence foodsafety.The annual Effects of Lead Exposure in the whole world
About 150,000 dead, the heavy metal pollutions such as " blood lead " event also occurs again and again in recent years in China." lead " is set to by World Health Organization
Cause one of ten kinds of chemicals of great public health concern.
The ground state electron of chromium is configured as [Ar] 4s13d5, altitude spin, therefore it can express different oxidation state, have
Extensive chemical and physical features.It can form acidity, alkalinity or amphoteric oxide according to oxidation state.When oxidation state is+3
(trivalent, Cr3+) and+6 (sexavalence, Cr6+) when it is most stable.Cr6+It is mainly used as the face of production textile dyestuff, paint, ink, plastics
Material, wood protection, chromium plating, steel and iron industry and process for tanning.The sewage of these industries is Cr in water body environment6+The main original of pollution
One of because.Cr6+Directly contact skin can occur different degrees of passivation, cause dermatitis, cutaneous necrosis and skin corrosion, there is research
It was found that often in contact with Cr6+The crowd of occupation, the cancered probability of respiratory system is larger, and height is insoluble and slightly solubility chromate is true
It is set to carcinogen.Cr6+Animal can also be made to generate physiological stress by destroying sperm and male reproductive system.In addition, Cr6+
It is also related with the Nutrient Absorption of plant and photosynthetic reduction, it is slow so as to cause plant growth, or even plant is made to wither
Yellow and necrosis phenomena.Due to Cr3+The toxicity and migration of ion are far smaller than Cr6+Ion, therefore using chemistry or biological reducing
Method is by Cr6+It is reduced to Cr3+It is the common method of current pollution of chromium reparation.
Currently, the administering method of heavy metal pollution mainly includes three categories: physical method, chemical method and bioanalysis.Physical method
It takes effect with chemical method relatively fast, but high expensive, is also easy to produce secondary pollution.Bioanalysis mainly utilizes plant and microorganism pair
The absorption and passivation of heavy metal.Wherein microbial treatment heavy metal pollution has at low cost, high efficiency, to environmental disruption
It is small, without secondary pollution, be suitable for the advantages such as large area repair by favor, have a good application prospect.Existing microorganism
Handle heavy metal lead ion (Pb2+) and chromium ion (Cr6+) efficiency can't be satisfactory.
Summary of the invention
The purpose of the present invention is to provide one plant from Xinjiang nuclear radiation pollution area screening, domestication after obtain have it is a variety of heavy
Metal tolerance, and phosphate metabolism mineralising precipitation of heavy metals lead ion (Pb can be passed through2+) and reduction of hexavalent chromium ion (Cr6 +) bacterium, can be used for the reparation of heavy metal pollution.
It is a further object of the present invention to provide the cultural method of above-mentioned bacterium, this method is simple and efficient.
Another object of the present invention is to provide application of the above-mentioned bacterium in lead, pollution of chromium are repaired.
The purpose of the present invention adopts the following technical scheme that realization.
The bacterium of one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion, classification naming are Su Yunjin gemma
HM-311 plants of bacillus (Bacillus thuringiensis), deposit number are as follows: GDMCC NO:60592.HM-311 plants be from
It separates, screen in Xinjiang nuclear radiation pollution area soil, then continuous passage culture is tamed under high-concentration lead ion existence condition
It obtains afterwards.
Separation, domestication and identification method it is as follows: will from Xinjiang nuclear radiation pollution area soil 10g be added to 90mL lead from
Enrichment culture in sub- isolation medium takes enrichment culture liquid to do 10 afterwards for 24 hours-2、10-3、10-4Gradient dilution respectively takes 0.1mL to distinguish
It is coated on lead ion isolation medium plate, 3 repetitions of each gradient, sets 30 DEG C, cultivates 2 days under the conditions of 200rpm.Wait grow
The bacterium colony difference streak inoculation that picking shape, size, color etc. are different after bacterium colony out is in corresponding plate, up to no miscellaneous bacteria falls.
Then the purifying strain inoculated of acquisition is put into 4 DEG C of refrigerators and is saved backup in solid LB media inclined-plane.By comparing, send out
Existing HM-31 bacterial strain has the characteristics that be resistant to various heavy pressure.By in the LB for containing high concentration (1000mg/L) lead ion
Continuous passage culture carrys out naturalized strain Bacillus thuringiensis HM-31 in culture medium, and condition of culture is 30 DEG C,
200rpm revolving speed shake culture, the passage of switching in every 12 hours is primary, after switching 30 times, obtains being resistant to high-concentration lead ion
The HM-311 strain of ability.The 16S rDNA sequence that HM-311 bacterial strain is obtained by PCR, as shown in SEQ ID NO:1.It is surveyed through sequence
Fixed and BLAST homology alignment and evolutionary analysis, the results showed that HM-311 bacterial strain and Bacillus thuringiensis
The homology of strain QZL38 16S rDNA and Bacillus thuringiensis strain c25 16S rDNA is
100%, determine that HM-311 bacterial strain is bacillus thuringiensis (Bacillus thuringiensis), therefore HM-311 bacterial strain is ordered
Entitled HM-31 plants of bacillus thuringiensis (Bacillus thuringiensis).
The present invention also provides the cultural methods of the bacterium, by golden bacillus (Bacillus thuringiensis)
HM-311 plants are inoculated into fermentation medium, shake culture under the conditions of 25-37 DEG C, revolving speed 100-200rpm.
In preferred technical solution, the fermentation medium is LB culture medium.
The present invention also provides application of the bacterium in lead, pollution of chromium are repaired.
In the present invention, the bacterium prepares with the following method: by golden bacillus (Bacillus
Thuringiensis it) is inoculated into fermentation medium for HM-311 plants, training is shaken under the conditions of 25-37 DEG C, revolving speed 100-200rpm
It supports.In preferred technical solution, the fermentation medium is LB culture medium.
The beneficial effects of the present invention are: the present invention obtains one plant after the separation of Xinjiang nuclear radiation pollution area, screening, domestication
Novel the bacterial strain of resistance to heavy metal HM-311 plants, which has mineralising precipitation of heavy metals lead ion (Pb2+) and reduction of hexavalent chromium ion
(Cr6+) ability, can be used for Heavy Metal Pollution Control.The bacterial strain is 2100mg/ to the highest tolerable concentration of heavy metal lead ion
L, the highest tolerable concentration to hexavalent chromium are 600mg/L.The fermented and cultured in the culture medium containing 1500mg/L lead ion
Two days, it can remove in culture medium 97% lead ion；Containing 100mg/L Cr6+Culture medium in fermented and cultured two days, can also
70% hexavalent chromium (Cr in former culture medium6+) ion.
Detailed description of the invention
Plumbum ion concentration is at any time in thalli growth situation and fermented liquid supernatant under the conditions of Fig. 1 1000mg/L plumbum ion concentration
Between change curve.
Plumbum ion concentration is at any time in thalli growth situation and fermented liquid supernatant under the conditions of Fig. 2 1500mg/L plumbum ion concentration
Between change curve.
Fig. 3 bacterium surface lead ion mineralization product scanning electron microscope analysis figure.
Fig. 4 bacterium surface lead ion mineralization product XRD analysis figure.
Hexavalent chromium concentration and total chromium concn change over time curve in Fig. 5 culture medium.
HM-311 bacterial strain of the present invention, classification naming are bacillus thuringiensis (Bacillus thuringiensis)
HM-311 plants, it is preserved in Guangdong Province's Culture Collection, deposit number are as follows: GDMCC NO:60592, preservation date
It is on 2 25th, 2019, depositary institution address are as follows: 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100 Guangdong Province microorganism is ground
Study carefully institute.
Specific embodiment
Embodiment 1: this example demonstrates that the culture medium that the present invention uses
LB culture medium: yeast powder 5g, peptone 10g, NaCl 10g and distilled water 1000mL are uniformly mixed, and obtain LB training
Support base, pH 7.2.
Lead ion isolation medium: Pb (NO is added in LB culture medium3)2So that Pb in culture medium2+It is final concentration of
1000mg/L is to get lead ion isolation medium.
Lead ion isolation medium plate: the agar of final concentration of 15g/L is added in lead ion isolation medium.
Embodiment 2: the acquisition of bacillus thuringiensis (Bacillus thuringiensis) HM-311
90mL lead ion isolation medium being added to from Xinjiang nuclear radiation pollution area soil 10g, (preparation method is shown in
Embodiment 1) in enrichment culture, take enrichment culture liquid to do 10 afterwards for 24 hours-2、10-3、10-4Gradient dilution respectively takes 0.1mL to be respectively coated
In on lead ion isolation medium plate (preparation method is shown in embodiment 1), 30 DEG C, 200rpm condition are set in 3 repetitions of each gradient
Lower culture 2 days.The difference streak inoculation of picking shape, size, color etc. are different after growing bacterium colony bacterium colony in corresponding plate,
Up to no miscellaneous bacteria falls.Then by the purifying strain inoculated of acquisition in solid LB media inclined-plane, be put into 4 DEG C of refrigerators save it is standby
With.By comparing, discovery HM-31 bacterial strain has the characteristics that be resistant to various heavy pressure.Therefore, to HM-31 bacterial strain carry out into
The research of one step.
By HM-31 bacterial strain in 30 DEG C of culture 12h, then identified.
The 16S rDNA sequence that HM-31 bacterial strain is obtained by PCR, as shown in SEQ ID NO:1.Through sequencing and
BLAST homology alignment and evolutionary analysis, the results showed that HM-31 bacterial strain and Bacillus thuringiensis strain
The homology of QZL38 16S rDNA and Bacillus thuringiensis strain c25 16S rDNA is 100%, really
Determining HM-31 bacterial strain is bacillus thuringiensis (Bacillus thuringiensis), therefore HM-31 Strain Designation is Su Yunjin
HM-31 plants of bacillus (Bacillus thuringiensis).
By in the LB culture medium containing 1000mg/L lead ion continuous passage culture come naturalized strain Bacillus
Thuringiensis HM-31, condition of culture are 30 DEG C, 200rpm revolving speed shake culture, and the passage of switching in every 12 hours is primary,
After switching 30 times, obtain with HM-311 plants of tolerance bacterial strain of high-concentration lead ion, through HM-311 plants of 16S rDNA of detection
Sequence is still as shown in SEQ ID NO:1, it was demonstrated that HM-311 plants are bacillus thuringiensis (Bacillus
Thuringiensis), it is named as HM-311 plants of bacillus thuringiensis (Bacillus thuringiensis).By Su Yunjin
HM-311 plants of bacillus (Bacillus thuringiensis) simultaneously send Guangdong Province's Culture Collection preservation.
Embodiment 3: the energy of resistance to heavy metal of HM-311 plants of bacillus thuringiensis (Bacillus thuringiensis)
Heavy metal ion Cu is added respectively in LB culture medium (fluid nutrient medium)2+、Zn2+、Cr6+、Cd2+、Ni2+、Co2+、
Pb2+, adjusting pH to 7.2, a variety of various concentrations of the addition concentration of every heavy metal species ion within the scope of 100-3000mg/L,
To investigate bacillus thuringiensis (Bacillus thuringiensis) HM-311 plants of tolerances to each metal ion species.
Not add the LB culture medium of metal ion as control.It is dense using the maximum metal ion concentration that bacterium can grow as maximum tolerance
Degree.After cultivating 48h, the growth (relative to culture original state) for adding bacterial biomass in heavy metal ion culture medium reaches pair
10% increased according to middle bacterial biomass, being considered as bacterium can grow.
Bacillus thuringiensis (Bacillus thuringiensis) HM-311 plants of maximal tolerable concentrations to each metal
As shown in table 1.As seen from Table 1, there is certain tolerance energy to metal ions such as lead, copper, zinc, chromium, cadmium, cobalt and nickel for HM-311 plants
Power, wherein it is especially prominent to lead ion tolerance, reach 2100mg/L.
1.HM-311 plants of the table maximal tolerable concentrations (MTC) to different metal ions
Embodiment 4: bacillus thuringiensis (Bacillus thuringiensis) HM-311 plants of precipitatings to lead ion
Removal capacity
(1) the HM-311 plants of removal capacities to 1000mg/L lead ion
Lead ion containing 1000mg/L is inoculated in (with Pb (NO by HM-31 plants of Bacillus thuringiensis3)2Shape
Formula addition) LB culture medium in, shake culture 60h under the conditions of 30 DEG C, revolving speed 200rpm, periodically sample, obtain fermentation liquid.It will
Fermentation liquid centrifugation removes thallus, plumbum ion concentration in test broth supernatant.
Test results are shown in figure 1, and by the culture of 48h, plumbum ion concentration is reduced to from 1000mg/L in culture medium
29.94mg/L, removal rate reach 97.01%.
(2) the HM-311 plants of removal capacities to 1500mg/L lead ion
Lead ion containing 1500mg/L is inoculated in (with Pb (NO by HM-31 plants of Bacillus thuringiensis3)2Shape
Formula addition) LB culture medium in, shake culture 60h under the conditions of 30 DEG C, revolving speed 200rpm, periodically sample, obtain fermentation liquid.It will
Fermentation liquid centrifugation removes thallus, plumbum ion concentration in test broth supernatant.
Test results are shown in figure 2, and by the culture of 60h, plumbum ion concentration is reduced to from 1500mg/L in culture medium
44.22mg/L, removal rate reach 97.05%.
Embodiment 5: HM-311 plants of bacillus thuringiensis (Bacillus thuringiensis) produce lead ion mineralising
The scanning electron microscope analysis of object
Bacterial strain HM-311 in LB culture medium and is added with 1000mg/L lead ion (with Pb (NO respectively3)2Form addition)
LB culture medium in cultivate, in culture 12h, for 24 hours, 36h and 48h take 1mL bacterium solution respectively, be centrifuged under the conditions of 4 DEG C, 5000rpm
After cleaning a thallus using sterile saline 0.5% glutaraldehyde solution is added, in 4 DEG C of conditions in 10 minutes collection thallus
Lower fixed 30min, then 4000rpm is centrifuged 1min, mistake under the conditions of 4 DEG C of the glutaraldehyde solution after discarding supernatant using 2.5%
Night is fixed, and then successively the cell after fixing is cleaned with 50%, 70%, 100% ethyl alcohol, by critical drying and metal spraying step
Afterwards, it is observed using scanning electron microscope and shoots photo.As a result as shown in figure 3, being trained in the culture medium for not adding lead ion
Feeding cell surface is completely smooth, and the bacterium cultivated after lead ion is added in culture medium, culture for 24 hours when, cell surface according to
It is so smooth, but when culture 36h, there is granular precipitate in cell surface, and with the increase of incubation time, granular precipitate
Also increase therewith.This phenomenon matches with lead ion subsidence curve in fermentation liquid.Thus speculate, before Bacteria Culture for 24 hours,
Bacterium carries out the growth accumulation of biomass, and after bacterial growth to the stage of stable development, cell is secreted extracellular substance and reacted with lead ion, raw
At granular precipitate.
Embodiment 6: HM-311 plants of bacillus thuringiensis (Bacillus thuringiensis) produce lead ion mineralising
The XRD analysis of object
Bacterial strain HM-311 is inoculated with respectively added with 0,150, in the LB culture medium of 1000mg/L lead ion, 30 DEG C,
It is cultivated 2 days under 200rpm revolving speed.Fermentation liquid is centrifuged 15 minutes separation of bacterial cells under the conditions of 4 DEG C, 5000rpm.Using cold
Lyophilizer is dehydrated cell, and XRD analysis is used for after being fully ground.XRD analysis uses Ni optical filter, Cu K alpha ray (λ
=0.1546nm), tube voltage 40kV, tube current 30mA, 20 °~60 ° of scanning range, 0.04 ° of scanning step.As a result such as Fig. 4 institute
Show, from spectrogram interpretation of result, it is found that cell surface particle contains 2 kinds of lead ore precipitatings, respectively Pb3(PO4)2、Pb5(PO4)3Cl。
Embodiment 7: HM-311 plants of bacillus thuringiensis (Bacillus thuringiensis) is to hexavalent chromium
Reducing property
Bacillus thuringiensis (Bacillus thuringiensis) is inoculated in LB culture medium for HM-31 plants, 30 DEG C,
Under the conditions of revolving speed 200rpm after shake culture 12h, hexavalent chromium is added into culture to final concentration of 100mg/L, is continued
Bacterial strain 60h is cultivated, periodically samples, obtains fermentation liquid.Fermentation liquid is centrifuged removal thallus, the Cr VI in test media supernatant
Ion and total chromium ion concentration, trivalent chromic ion concentration=total chromium ion concentration-hexavalent chromium concentration.
Test results are shown in figure 5, and by the culture of 48h, hexavalent chromium concentration is reduced to from 100mg/L in culture medium
28.2mg/L, reduction rate reach 71.8%.
SEQUENCE LISTING
<110>Nanjing University of Technology
Nanjing Normal University
Bacterium and its application of<120>one plants of mineralising precipitation of heavy metals lead ions and reduction of hexavalent chromium ion
<130> 20190531
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1540
<212> DNA
<213>HM-311 plants of bacillus thuringiensis (Bacillus thuringiensis)
<400> 1
agagtttgat cctggctcag gatgaacgct ggcggcgtgc ctaatacatg caagtcgagc 60
gaatggatta agagcttgct cttatgaagt tagcggcgga cgggtgagta acacgtgggt 120
aacctgccca taagactggg ataactccgg gaaaccgggg ctaataccgg ataacatttt 180
gaaccgcatg gttcgaaatt gaaaggcggc ttcggctgtc acttatggat ggacccgcgt 240
cgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcgtag ccgacctgag 300
agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta 360
gggaatcttc cgcaatggac gaaagtctga cggagcaacg ccgcgtgagt gatgaaggct 420
ttcgggtcgt aaaactctgt tgttagggaa gaacaagtgc tagttgaata agctggcacc 480
ttgacggtac ctaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt 540
aggtggcaag cgttatccgg aattattggg cgtaaagcgc gcgcaggtgg tttcttaagt 600
ctgatgtgaa agcccacggc tcaaccgtgg agggtcattg gaaactggga gacttgagtg 660
cagaagagga aagtggaatt ccatgtgtag cggtgaaatg cgtagagata tggaggaaca 720
ccagtggcga aggcgacttt ctggtctgta actgacactg aggcgcgaaa gcgtggggag 780
caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta agtgttagag 840
ggtttccgcc ctttagtgct gaagttaacg cattaagcac tccgcctggg gagtacggcc 900
gcaaggctga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt 960
aattcgaagc aacgcgaaga accttaccag gtcttgacat cctctgaaaa ccctagagat 1020
agggcttctc cttcgggagc agagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg 1080
tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg atcttagttg ccatcattaa 1140
gttgggcact ctaaggtgac tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa 1200
tcatcatgcc ccttatgacc tgggctacac acgtgctaca atggacggta caaagagctg 1260
caagaccgcg aggtggagct aatctcataa aaccgttctc agttcggatt gtaggctgca 1320
actcgcctac atgaagctgg aatcgctagt aatcgcggat cagcatgccg cggtgaatac 1380
gttcccgggc cttgtacaca ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg 1440
gtggggtaac ctttttggag ccagccgcct aaggtgggac agatgattgg ggtgaagtcg 1500
taacaaggta gccgtatcgg aaggtgcggc tggatcacct 1540
Claims (6)
1. the bacterium of one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion, classification naming is Su Yunjin gemma bar
Bacterium (Bacillus thuringiensis) HM-311 plants, deposit number are as follows: GDMCC NO:60592.
2. the cultural method of bacterium described in claim 1, it is characterised in that by golden bacillus (Bacillus thuringiensis) HM-311 plants be inoculated into fermentation medium, under the conditions of 25-37 DEG C, revolving speed 100-200rpm shake training
It supports.
3. cultural method as claimed in claim 2, which is characterized in that the fermentation medium is LB culture medium.
4. application of the bacterium described in claim 1 in lead, pollution of chromium are repaired.
5. applying according to claim 4, it is characterised in that the bacterium prepares with the following method: by golden bacillus
(Bacillus thuringiensis) HM-311 plants be inoculated into fermentation medium, in 25-37 DEG C, revolving speed 100-200rpm item
Shake culture under part.
6. applying according to claim 5, it is characterised in that the fermentation medium is LB culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910476990.9A CN110079487B (en) | 2019-06-03 | 2019-06-03 | Bacterium for mineralizing and precipitating heavy metal lead ions and reducing hexavalent chromium ions and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910476990.9A CN110079487B (en) | 2019-06-03 | 2019-06-03 | Bacterium for mineralizing and precipitating heavy metal lead ions and reducing hexavalent chromium ions and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110079487A true CN110079487A (en) | 2019-08-02 |
| CN110079487B CN110079487B (en) | 2021-01-05 |
Family
ID=67423285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910476990.9A Active CN110079487B (en) | 2019-06-03 | 2019-06-03 | Bacterium for mineralizing and precipitating heavy metal lead ions and reducing hexavalent chromium ions and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110079487B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471608A (en) * | 2019-11-28 | 2020-07-31 | 中南林业科技大学 | Strain capable of adsorbing manganese and promoting growth of plants and application thereof |
| CN116144535A (en) * | 2022-12-13 | 2023-05-23 | 中国科学院地球化学研究所 | Bacillus, microbial inoculum and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150070461A (en) * | 2013-12-16 | 2015-06-25 | 재단법인 전주생물소재연구소 | Bacillus thuringiensis JB-007 strain having sorption of anionic dye and metal, and biomass using the same |
| CN106277586A (en) * | 2016-08-24 | 2017-01-04 | 宁波枫叶杰科生物技术有限公司 | A kind of quick improvement water body pollution of chromium also reclaims the microbial process of chromium element |
| CN106337033A (en) * | 2016-11-09 | 2017-01-18 | 南京工业大学 | Bacterium for adsorbing heavy metals cadmium and copper and application thereof |
| CN106676045A (en) * | 2017-02-16 | 2017-05-17 | 武汉科技大学 | Antibiotic-resistant hexavalent chromium reducing bacterium and application thereof |
| CN108587972A (en) * | 2018-05-11 | 2018-09-28 | 北京林业大学 | Adsorb bacillus thuringiensis MRP-3 and its application of heavy metal |
-
2019
- 2019-06-03 CN CN201910476990.9A patent/CN110079487B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150070461A (en) * | 2013-12-16 | 2015-06-25 | 재단법인 전주생물소재연구소 | Bacillus thuringiensis JB-007 strain having sorption of anionic dye and metal, and biomass using the same |
| CN106277586A (en) * | 2016-08-24 | 2017-01-04 | 宁波枫叶杰科生物技术有限公司 | A kind of quick improvement water body pollution of chromium also reclaims the microbial process of chromium element |
| CN106337033A (en) * | 2016-11-09 | 2017-01-18 | 南京工业大学 | Bacterium for adsorbing heavy metals cadmium and copper and application thereof |
| CN106676045A (en) * | 2017-02-16 | 2017-05-17 | 武汉科技大学 | Antibiotic-resistant hexavalent chromium reducing bacterium and application thereof |
| CN108587972A (en) * | 2018-05-11 | 2018-09-28 | 北京林业大学 | Adsorb bacillus thuringiensis MRP-3 and its application of heavy metal |
Non-Patent Citations (2)
| Title |
|---|
| ZHUANG, L等: "Cost-effective production of Bacillus thuringiensis biopesticides by solid-state fermentation using wastewater sludge:Effects of heavy metals", 《BIORESOURCE TECHNOLOGY》 * |
| 魏斐: "苏云金芽孢杆菌还原六价铬离子的特性研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471608A (en) * | 2019-11-28 | 2020-07-31 | 中南林业科技大学 | Strain capable of adsorbing manganese and promoting growth of plants and application thereof |
| CN111471608B (en) * | 2019-11-28 | 2022-09-13 | 中南林业科技大学 | Strain capable of adsorbing manganese and promoting growth of plants and application thereof |
| CN116144535A (en) * | 2022-12-13 | 2023-05-23 | 中国科学院地球化学研究所 | Bacillus, microbial inoculum and application thereof |
| CN116144535B (en) * | 2022-12-13 | 2023-09-26 | 中国科学院地球化学研究所 | Bacillus, microbial inoculum and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110079487B (en) | 2021-01-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| CN111423996B (en) | Complex microbial inoculant, preparation method thereof and application thereof in soil remediation field | |
| Watanabe et al. | Biosorption of cadmium ions using a photosynthetic bacterium, Rhodobacter sphaeroides S and a marine photosynthetic bacterium, Rhodovulum sp. and their biosorption kinetics | |
| CN105670980B (en) | A kind of application of the microbial strains of restoration of soil polluted by heavy metal | |
| CN105802890B (en) | A kind of achromobacter CZ207 bacterial strain of preventing from heavy metal promoting growth of plants and application thereof | |
| CN105132315B (en) | High mountain bacillus altitudinis YLX-5 and its application | |
| CN105132317B (en) | Aoxidize microbacterium Microbacterium oxydans YLX-2 and its application | |
| CN110078220B (en) | Method and strain for in-situ remediation of arsenic-polluted high-saline water by using blue-green algae | |
| CN112592855B (en) | Bacillus subtilis and method for treating uranium and cadmium polluted water body by using same | |
| CN104531585A (en) | Delftia tsuruhatensis and application thereof | |
| CN106434470A (en) | Polycyclic aromatic hydrocarbon degrading bacterium and applications thereof | |
| CN100560713C (en) | The Achromobacter xylosoxidans SY8 and the purposes of purifying arsenic contamination | |
| CN105969759A (en) | Method for immobilized culture of salt-tolerant bacillus strains and application | |
| CN104388328B (en) | Strain for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons and obtaining method and application thereof | |
| CN110079487A (en) | Bacterium and its application of one plant of mineralising precipitation of heavy metals lead ion and reduction of hexavalent chromium ion | |
| CN103451105B (en) | A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application | |
| CN109554298A (en) | The enrichment method of a kind of microbial flora and its in the processing heavy metal-polluted application caught of Cr | |
| CN111117909B (en) | Strain capable of resisting multiple heavy metals and promoting plant growth and application thereof | |
| CN110076193A (en) | Lebanon pseudomonas strains MY and its application in the reparation of heavy metal pollution salt-affected soil | |
| CN114107101B (en) | Cadmium and chromium-fixed cupronickel bacterium and application thereof in co-remediation of cadmium and chromium composite polluted soil | |
| CN105670977B (en) | One plant of enterobacteria and its application | |
| CN105861362B (en) | The actinomycetes strain of one plant of resistance to heavy metal and its application | |
| Jaiswal et al. | Prospects of a hot spring–originated novel cyanobacterium, Scytonema ambikapurensis, for wastewater treatment and exopolysaccharide-enriched biomass production | |
| CN117844712A (en) | Rhodococcus and application thereof in degradation of polycyclic aromatic hydrocarbon | |
| CN108004271B (en) | Streptomyces with algae-lysing activity and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |