CN109092873B - Method for repairing arsenic-polluted soil by using plant-microorganism combination - Google Patents
Method for repairing arsenic-polluted soil by using plant-microorganism combination Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
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- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Abstract
The invention discloses a method for repairing arsenic-polluted soil by using plant-microorganism combination, which comprises the following steps: firstly, screening plants and microorganisms to obtain plant pinwheel grass with strong arsenic resistance and microorganism staphylococcus saprophyticus with strong arsenic resistance, then planting the plant pinwheel grass with strong arsenic resistance in soil polluted by heavy metal arsenic, adding the arsenic-resistant microorganism staphylococcus saprophyticus in rhizosphere soil of an arsenic-enriched plant, conventionally culturing, watering and moisturizing, repairing arsenic in the enriched soil by combining the absorption effect of the arsenic-enriched plant on the arsenic and the strengthening effect of the arsenic-resistant microorganism, and repeating the process to achieve the purpose of repairing the arsenic-polluted soil in a tailing area. The soil remediation method has the advantages of good effect, low cost, easy management and operation, no secondary pollution and wide application prospect.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to a method for remediating arsenic-contaminated soil by using a plant-microorganism combination.
Background
The problem of heavy metal pollution of soil is an environmental problem concerned in the global scope, and the data shows that the total exceeding standard rate of the soil heavy metals in China is 16.1 percent, and the soil heavy metal pollution is mainly pollution of six heavy metals of cadmium, nickel, copper, arsenic, mercury and lead. Wherein, arsenic is a toxic and carcinogenic chemical element, the distribution is very wide, the main source of arsenic in soil is agriculture and industry, the arsenic has great harm to environment, animals and plants, and even can enter human body through food chain to cause chronic poisoning of human body, therefore, the arsenic pollution becomes an environmental problem which harms the globality. Soil is an important component of human living environment, arsenic has the characteristics of concealment, accumulation, irreversibility, long-term property and the like in the soil, and has great harm to soil fertility, the living growth of animals and plants and human health, so that the remediation of the soil arsenic pollution is urgent.
The conventional arsenic pollution treatment method is to change the cultivation mode, wash soil, add antagonists, modifiers and other theories, can play a certain role, but has the hidden danger of secondary pollution, large engineering quantity, time and labor consumption and difficulty in fundamentally eliminating the harm caused by arsenic. The ecological restoration technology overcomes the defects and becomes a hotspot and a difficulty of research, the plant-microorganism cooperative restoration technology is the bioremediation technology with the most development potential at present, the advantages of the plants and the microorganisms can be fully exerted, the advantages of the plants and the microorganisms are made full of, the restoration efficiency of the polluted soil is improved, the secondary pollution is avoided, and the purpose of thoroughly restoring the polluted soil is finally achieved. In recent years, a large amount of researches on arsenic-polluted plant and microorganism combined remediation technologies are carried out by scholars at home and abroad, and abundant research results are obtained, but the researched plants are mostly concentrated on ciliate desert-grass, and the research on the piniperbus alternatus is rarely reported.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a method for repairing arsenic-polluted soil by using a plant-microorganism combination.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for remediating arsenic-contaminated soil by using a plant-microorganism combination comprises the following steps: planting arsenic-enriched plant pinus sylvestris in arsenic-polluted soil, preparing bacterial liquid from staphylococcus saprophyticus CP3, irrigating roots of the bacterial liquid 7 days after the pinus sylvestris is planted, inoculating the bacterial liquid into the rhizosphere soil of the arsenic-enriched plant pinus sylvestris, inoculating 15-20 mL bacterial liquid per kilogram of soil for 1-3 times, and enriching arsenic in the soil by combining the absorption effect of the arsenic-enriched plant pinus sylvestris on arsenic and the strengthening effect of arsenic-resistant bacteria staphylococcus saprophyticus CP 3.
The invention is also characterized in that the arsenic-enriched plant pinus alternatus is obtained by screening through the following method:
selecting herbaceous plants with good growth vigor in arsenic-polluted areas, namely Bidens bipinnata, Artemisia capillaris, ciliate desert-grass, pinto, quinoa, Ixeris denticulata, ageratum thistle, cotton rose hibiscus, brick seedlings, buddleia latifolia, ramie, juncus effuses and Fimbristylis, and digging up the two with root soil; separating the rhizosphere soil of each herbaceous plant and each herbaceous plant to respectively obtain each herbaceous plant material and each soil sample; pretreating each soil sample and each herbaceous plant material respectively, determining the pH value of each soil sample, determining the arsenic content in each soil sample and each herbaceous plant material, calculating the migration rate and enrichment rate of each herbaceous plant material to arsenic, and screening out the herbaceous plant windmill grass with the strongest arsenic resistance according to the determination result.
The invention is also characterized in that the arsenic-resistant bacteria staphylococcus saprophyticus CP3 is obtained by screening through the following method:
under aseptic conditions, 10g of the pretreated soil sample with the highest arsenic concentration was added to 90mL of sterile water containing glass beads, shaken up with a vortex shaker, 1mL was added to 9mL of sterile water, and then diluted to a concentration of 10-6、10-7、10-8Respectively coating 200 mu L of the suspension liquid on an LB solid culture medium, repeating three times for each gradient, culturing in an incubator at 37 ℃, and selecting single colonies with obviously different shapes for streak culture after the colonies grow out to obtain primary-screened bacteria; inoculating the primary screened bacteria into an LB liquid culture medium, culturing for 12h at 37 ℃ and 180rpm, centrifuging by using a sterilized centrifuge tube at 10000rpm, pouring out supernatant, and draining to obtain thalli; then 100mL of arsenic with the concentration of 5 mg.L is prepared-1After sterilization, weighing 1g of thallus in a super clean bench, adding the thallus into a culture medium, culturing at 37 ℃ and 180rpm for 12h, centrifuging at 10000rpm, measuring the concentration of arsenic in a supernatant, screening out a microorganism CP3 with the strongest arsenic resistance according to the measurement result, continuously purifying a single colony for 4 times by adopting a flat line scribing separation method, and identifying the microorganism as staphylococcus saprophyticus CP 3.
The invention is also characterized in that the pretreatment of each soil sample specifically comprises the following steps: and (3) air-drying each soil sample in a cool and dry place, removing stones, putting the soil samples into a mortar, grinding the soil samples through a 0.15mm sieve, drying the sieved samples, and putting the samples into a dryer for later use.
The invention is also characterized in that the pretreatment of each herbaceous plant material specifically comprises the following steps: cleaning each herbaceous plant material, airing, drying for 48 hours at 50 ℃, then respectively crushing the roots and the overground parts of the herbaceous plant materials, and separately storing the crushed samples for later use.
The invention is also characterized in that the bacterial liquid is prepared by inoculating staphylococcus saprophyticus CP3 into 100mL LB liquid culture medium and culturing for 12 h.
The invention is also characterized in that nutrient solution is added after the windmill grass is planted, the nutrient solution is supplemented after 10 days, and watering is carried out regularly to keep the water content of the arsenic polluted soil to be 60-80% of the maximum water holding capacity.
The invention is also characterized in that the nutrient solution is a water and soil universal nutrient solution sold in the market.
The invention has the advantages and positive effects that:
(1) according to the method for repairing arsenic-polluted soil by using the combination of the plants and the microorganisms, herbaceous plants with good growth vigor in arsenic-polluted areas are used as arsenic-resistant plants for screening, so that the method is strong in adaptability to the soil environment of the polluted areas;
(2) the invention adopts the plant windmill grass with strong arsenic resistance, has vigorous growth and larger plant shape, and is beneficial to water and soil conservation while repairing the arsenic-polluted soil;
(3) the arsenic-resistant microorganisms are derived from herbaceous plant rhizosphere soil in arsenic-polluted areas, are good in compatibility with arsenic-resistant plants, and are strong in environmental adaptability to polluted areas;
(4) the plant-microorganism soil remediation method provided by the invention has the advantages of good effect, low cost, easiness in management and operation, no secondary pollution and wide application prospect.
Drawings
FIG. 1 is a graph of the mobility and enrichment of plants for arsenic.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the respective manufacturers.
The screened Staphylococcus saprophyticus (Staphylococcus saprophyticus) CP3 is the existing strain of the microorganism strain preservation management center, does not relate to the development of new strains, and only relates to the application thereof. The Staphylococcus saprophyticus CP3 screened in the following examples is specifically a strain with a preservation number of CCTCC No. m205145 from Chinese Center for Type Culture Collection (CCTCC).
The pot experiment in the following examples was carried out in a greenhouse of river basin academy, test windmill grass was purchased from a certain flower market, test soil was collected from a park not contaminated by heavy metals, the test soil was air-dried and crushed and sieved with a 3mm sieve, the upper opening diameter, the lower opening diameter and the pot height of the test flowerpot were 15, 10 and 10cm, respectively, and the nutrient solution was a commercially available water and soil general-purpose nutrient solution.
Example 1 plant selection
Selecting herbaceous plants with good growth vigor in a certain tailing area of the river basin in Guangxi, such as spanishneedles herb, south wormwood herb, centipede herb, windmill grass, goosegrass, sowthistle herb, ageratum, cotton rose, brick seedlings, buddleia latifolia, ramie, juncus effuses and Fimbristylis, digging up the plants together with root and soil, and selecting 3 plants with similar plant heights as parallel samples from each herbaceous plant; separating the rhizosphere soil of each herbaceous plant and each herbaceous plant to respectively obtain each herbaceous plant material and each soil sample; air-drying each soil sample in a cool and dry place, removing stones, putting the soil samples into a mortar, grinding the soil samples through a 0.15mm sieve, and drying the soil samples for later use; cleaning and airing each herbaceous plant material, measuring the plant height and fresh weight of the herbaceous plant material, drying the herbaceous plant material at 50 ℃ for 48 hours, respectively measuring the dry weight of the root and the overground part of each herbaceous plant material, then crushing the root and the overground part of each herbaceous plant material, and separately storing the crushed samples;
weighing 10g of each soil sample subjected to air drying, grinding and drying treatment, respectively placing the soil samples into 50mL beakers, adding 25mL of deionized water, stirring and standing for 30min, measuring the pH value of the soil by using a pH meter, repeating the steps for 3 samples, and taking an average value of the results.
Respectively adding the weighed and processed soil samples and herb plant materials into a polytetrafluoroethylene digestion tube, adding a small amount of water for wetting, adding 16mL of nitric acid and 4mL of perchloric acid, covering a cover, uniformly shaking, placing for 24 hours, placing in a digestion instrument for heating for 1 hour, removing the cover, and continuing to heat; if the volume of the sample is reduced or the sample is blackened, adding 2mL of nitric acid, continuing to heat the sample, controlling the temperature below 200 ℃ (the sample is lost when the temperature exceeds 200 ℃), taking down the residual offwhite residue in the tube, cooling the offwhite residue, dissolving the residue with 1mL of 50% nitric acid, transferring the dissolved residue into a 50mL colorimetric tube, cleaning the pipe with ultrapure water, finally fixing the volume with the ultrapure water, and measuring the concentration of arsenic in each soil sample and each herbaceous plant material by using an atomic fluorescence photometer.
All detection treatments were set to 3 replicates and the data were analyzed for significance of difference using SPSS 20.0.
And (3) test results:
(1) effect of arsenic contamination on plant Biomass
The results of measuring the biomass of each herbaceous plant material in the tailing area are shown in table 1, and it can be seen from table 1 that the growth differences of various plants distributed in the tailing area are obvious. Wherein, the fresh weights of the three plants of ageratum conyzoides, cotton rose hibiscus and centipede grass are all lower than 20g, which indicates that the biomass of the three plants is relatively low.
TABLE 1 measurement results of plant Biomass
(2) Effect of arsenic contamination on plant rhizosphere soil pH
The results of measuring the pH of the rhizosphere soil sample of the plant material in the tailing area 13 are shown in Table 2, and it can be seen from Table 2 that the rhizosphere soil of the dominant plant in the tailing area is almost all weakly acidic, which indicates that the heavy metal ions in the soil are in an active state and are easily absorbed and transformed by the plant body.
TABLE 2 Table of pH measurement results of plant rhizosphere soil
(3) Tailing area plant and determination of arsenic concentration of rhizosphere soil thereof
The method comprises the steps of numbering 13 plant samples in a tailing area in sequence, wherein the corresponding sample numbers are 1-13, measuring the arsenic concentrations of 13 herbaceous plants and rhizosphere soil samples of the herbaceous plants, and determining the arsenic concentrations of the 13 herbaceous plants and the rhizosphere soil samples of the herbaceous plants, wherein the results are shown in table 3, as can be seen from table 3, the difference between the arsenic concentrations of the 13 herbaceous plants in the tailing area and the corresponding rhizosphere soil is large, and the comparison shows that the arsenic concentrations of overground parts of six herbaceous plants (namely, a sample 1, a sample 3, a sample 4, a sample 9, a sample 11 and a sample 12) are higher than the arsenic concentrations of underground parts of the six herbaceous plants, so that the six herbaceous plants have certain migration and transformation capacity on arsenic.
TABLE 3 table of determination results of arsenic concentration in plants and soil samples in tailing area
(4) Determination of migration rate and enrichment rate of plants to arsenic in soil of tailing area
The arsenic migration and enrichment of 13 herbaceous plants in the tailing area were calculated according to table 3, and the results are shown in fig. 1. Hyper-enriched plants have two basic characteristics: the concentration of heavy metal in the overground part of the plant is greater than that in the underground part of the root system; the total heavy metal content of the plant body is larger than that of the rhizosphere soil. By analyzing the data in fig. 1, only ciliate desert-grass and ledum chartarum (sample 9 and sample 11) satisfying the above conditions and having high mobility and enrichment rate can be obtained. The mobility and the enrichment rate of the pinus avicens (sample 11) are both better than those of the ciliate desert-grass, the enrichment rate can reach 589 percent, and the mobility can reach 67 percent, so that the herbaceous plant with the strongest arsenic resistance is screened out to be the pinus avicens.
Example 2 screening of microorganisms
Under aseptic conditions, 10g of the soil sample pretreated in example 1 and having the highest arsenic concentration was added to 90mL of sterile water containing glass beads, shaken up with a vortex shaker, 1mL was added to 9mL of sterile water, and then diluted to a concentration of 10-6、10-7、10-8Respectively taking 200 mu L of the soil suspension, coating the soil suspension on an LB solid culture medium, repeatedly arranging three gradients in each gradient, culturing in an incubator at 37 ℃, selecting single colonies with obviously different appearances after the colonies grow out, and carrying out streak culture to obtain 10 primary-screened bacteria with obviously different appearances, respectively marking as CP1-CP10, and marking as CK by taking the LB culture medium containing arsenic without the bacteria as a contrast; inoculating the obtained primary-screened bacteria CP1-CP10 into an LB liquid culture medium, culturing at 37 ℃ and 180rpm for 12h, centrifuging by using a sterilized centrifugal tube at 10000rpm, pouring out supernatant, and draining to obtain thalli; then 100mL of arsenic with the concentration of 5 mg.L is prepared-1LB liquid Medium ofAfter the bacteria are cultured, 1g of the bacteria are weighed in a super clean bench and added into a culture medium, LB culture medium containing arsenic without the bacteria is used as a reference and is marked as CK, the bacteria are cultured for 12 hours at 37 ℃ and 180rpm, then centrifugation is carried out at 10000rpm, and the concentration of the arsenic in the supernatant is measured by using an atomic fluorescence photometer.
And (3) test results:
the arsenic concentration in the supernatant, as determined by atomic fluorescence photometry, is shown in Table 4. As can be seen from Table 4, 10 strains all have a certain enrichment effect on arsenic, because in a liquid, the surfaces of the strains contain various polar functional groups, which can provide adsorption active sites to combine with arsenic ions, and dead bacteria can also adsorb heavy metals through some chemical groups on the surfaces of cell walls, so that the arsenic concentration in a culture medium is reduced, and experimental results show that CP3 bacteria has the best enrichment ability on arsenic, so that the microorganism with the strongest arsenic resistance is selected to be CP3, a single colony of the microorganism is purified continuously for 4 times by adopting a flat plate line scribing separation method, and the microorganism is identified to be CP3 which is Staphylococcus saprophyticus (Staphylococcus saprophyticus) CP 3.
TABLE 4 measurement results of arsenic concentration in the supernatant of the cells
Description of the drawings: in comparison of the difference of the enrichment effect of 10 strains on arsenic, different letters in the same column data indicate that the difference is significant (P < 0.05).
Example 3 Combined plant-microbial remediation
The microorganism CP3 in example 2 was inoculated into 100mL LB liquid medium and cultured for 12 hours to obtain CP3 bacterial liquid for use.
1kg of uncontaminated soil was added to the pot, followed by 1 mg. multidot.mL-1The arsenic standard solution of (1) is prepared so that the concentration of arsenic in potting soil is 5 mg/kg-1Planting the same-growth windmill grass, adding 50mL of prepared nutrient solution, seedling-recovering for 7 days, adding 20mL of CP3 bacterial liquid into the rhizosphere soil of windmill grass, using the windmill grass without bacteria as contrast, recording as CK, repeating every group for three times, and then plantingWatering for 1 time every two days, keeping water content (the water addition amount is kept at 60% of the field water holding amount), adding 1 time of nutrient solution after 10 days, removing the whole plant of the windmill grass after 60 days, cleaning, and adding 5 mmol.L-1Soaking the root in calcium nitrate solution for 15min, washing with deionized water, deactivating enzyme, oven drying, measuring biomass, plant height and arsenic concentration of herba Pteridis Multifidae, and measuring arsenic concentration in soil.
Pot experiment results:
the results of biomass measurement before and after the windgrass test are shown in table 5. After the cultivation, the plant height and fresh weight of the windmill grass are increased, and the windmill grass can grow in a set cultivation environment. The data result shows that the plant height and fresh weight of the test group added with the strain are higher than those of the control group, and the test group added with the strain proves that the test group added with the strain is beneficial to the growth of the pinus alternatus linn, because the metabolic activity of microorganisms can improve the physicochemical property of soil to a certain extent, can produce some auxin substances and promotes the growth of plants.
TABLE 5 Biomass measurement results before and after the windmill grass test
The results of measuring the arsenic concentration, the mobility and the enrichment rate of the windmill grass and the soil are shown in table 6, and it can be seen from table 6 that after a period of cultivation, part of the arsenic added into the soil is absorbed by the windmill grass, and the control group without adding bacteria also meets the conditions of super-enriched plants, but the mobility and the enrichment rate of the test group with adding bacteria are improved, so that the addition of the strain is more beneficial to the migration and the enrichment of the windmill grass on the arsenic. The metabolic activity of the microorganisms can improve the physicochemical property of the soil to a certain extent, promote the absorption and accumulation of heavy metals in the soil by plants, improve the remediation efficiency and achieve the aim of rapidly remedying the arsenic-polluted soil.
TABLE 6 arsenic concentration, mobility and enrichment ratio in windmill grass and soil
Description of the drawings: in the comparison of the difference of the arsenic enrichment effect of the pot culture tests of the windmill grass and the microorganism in different combination modes, the difference of significance (P < 0.05) is shown by different letters in the same column of data.
In conclusion, the plant-microorganism combined enrichment of arsenic in soil adopted by the invention has a good effect, arsenic in soil can be extracted by utilizing the arsenic enrichment capacity of the windmill grass, the arsenic absorption capacity of the windmill grass can be effectively improved by the aid of the strengthening effect of arsenic-resistant bacteria, so that the arsenic super-enrichment plant can be labeled and applied to soil remediation, the process of the invention is repeated, the windmill grass-microorganism combined effect continuously absorbs arsenic in soil until the arsenic content in the soil reaches the environmental safety standard, and the purpose of soil remediation is further achieved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A method for remediating arsenic-contaminated soil by using plant-microorganism combination is characterized by comprising the following steps: planting arsenic-enriched plant pinus sylvestris in arsenic-contaminated soil, preparing staphylococcus saprophyticus CP3 into bacterial liquid, irrigating roots of the bacterial liquid into rhizosphere soil of the arsenic-enriched plant pinus sylvestris 7 days after the pinus sylvestris is planted, inoculating 15-20 mL of the bacterial liquid per kilogram of soil for 1-3 times, and enriching arsenic in the soil by combining the absorption effect of the arsenic-enriched plant pinus sylvestris on arsenic and the strengthening effect of arsenic-resistant bacteria staphylococcus saprophyticus CP 3;
the arsenic-enriched plant pinus alternatus is obtained by screening through the following method:
selecting herbaceous plants with good growth vigor in arsenic-polluted areas, namely Bidens bipinnata, Artemisia capillaris, ciliate desert-grass, pinto, quinoa, Ixeris denticulata, ageratum thistle, cotton rose hibiscus, brick seedlings, buddleia latifolia, ramie, juncus effuses and Fimbristylis, and digging up the two with root soil; separating the rhizosphere soil of each herbaceous plant and each herbaceous plant to respectively obtain each herbaceous plant material and each soil sample; respectively pretreating each soil sample and each herbaceous plant material, determining the pH value of each soil sample, determining the arsenic content in each soil sample and each herbaceous plant material, calculating the mobility and enrichment rate of each herbaceous plant material to arsenic, and screening out herbaceous plant pinus sylvestris with the strongest arsenic resistance according to the determination result;
the arsenic-resistant bacteria staphylococcus saprophyticus CP3 is obtained by screening through the following method:
under aseptic conditions, 10g of the pretreated soil sample with the highest arsenic concentration was added to 90mL of sterile water containing glass beads, shaken up with a vortex shaker, 1mL was added to 9mL of sterile water, and then diluted to a concentration of 10-6、10-7、10-8Respectively coating 200 mu L of the suspension liquid on an LB solid culture medium, repeating three times for each gradient, culturing in an incubator at 37 ℃, and selecting single colonies with obviously different shapes for streak culture after the colonies grow out to obtain primary-screened bacteria; inoculating the primary screened bacteria into an LB liquid culture medium, culturing for 12h at 37 ℃ and 180rpm, centrifuging by using a sterilized centrifuge tube at 10000rpm, pouring out supernatant, and draining to obtain thalli; then 100mL of arsenic with the concentration of 5 mg.L is prepared-1After sterilization, weighing 1g of thallus in a super clean bench, adding the thallus into a culture medium, culturing at 37 ℃ and 180rpm for 12h, centrifuging at 10000rpm, measuring the concentration of arsenic in a supernatant, screening out a microorganism CP3 with the strongest arsenic resistance according to the measurement result, continuously purifying a single colony for 4 times by adopting a flat line scribing separation method, and identifying the microorganism as staphylococcus saprophyticus CP 3.
2. The method for remediating arsenic-contaminated soil by using a plant-microorganism combination as claimed in claim 1, wherein the pretreatment of each soil sample comprises the following steps: and (3) air-drying each soil sample in a cool and dry place, removing stones, putting the soil samples into a mortar, grinding the soil samples through a 0.15mm sieve, drying the sieved samples, and putting the dried samples into a dryer for later use.
3. The method for remediating arsenic contaminated soil using a combination of plant and microorganism as claimed in claim 1, wherein the pre-treatment of each herbaceous plant material comprises the following steps: cleaning the herbaceous plant material, drying at 50 deg.C for 48 hr, crushing the root and aerial parts of the plant, and storing the crushed samples separately.
4. The method for remediating arsenic-contaminated soil by using a plant-microorganism combination as claimed in claim 1, wherein the bacterial solution is prepared by inoculating Staphylococcus saprophyticus CP3 into 100mL LB liquid medium and culturing for 12 h.
5. The method for remediating arsenic-contaminated soil using a combination of plant and microorganism as set forth in claim 1, wherein the windgrass is cultured in the arsenic-contaminated soil under the conditions: after the windmill grass is planted, the nutrient solution is added, the nutrient solution is supplemented after 10 days, and watering is carried out regularly to keep the water content of the arsenic-polluted soil to be 60-80% of the maximum water holding capacity.
6. The method for remediating arsenic-contaminated soil using a plant-microorganism combination as claimed in claim 5, wherein said nutrient solution is a commercially available water and soil general-purpose nutrient solution.
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