CN110241111B - Method for enhancing bacterial chromium reduction capacity by modified activated carbon immobilized cells - Google Patents
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Abstract
The invention discloses a method for enhancing micrococcus chromium reduction capacity by modified activated carbon immobilized cells, and belongs to the technical field of environmental remediation. Major parameters of immobilized pellet reagent: sodium alginate (3%, w/v), calcium chloride (4%, w/v), micrococcus bacteria liquid and modified activated carbon (0.1-0.5 g), uniformly mixing the sodium alginate solution, micrococcus bacteria liquid and modified activated carbon, dripping the calcium chloride solution for crosslinking reaction, standing for four hours at room temperature, and adding the mixture into hexavalent chromium solution for reduction reaction. The reduction effect of hexavalent chromium added with 0.1-0.5g of modified activated carbon is compared with that of the hexavalent chromium added with no modified activated carbon, and the reduction rate is improved by 8.4-13.4%. The invention utilizes the adsorption characteristic of the activated carbon, can promote the immobilized bacteria to efficiently reduce hexavalent chromium ions in the water body, and remarkably improves the reduction efficiency of micrococcus on hexavalent chromium in the water body.
Description
Technical Field
The invention belongs to the technical field of environmental remediation, and particularly relates to a method for enhancing bacterial chromium reduction capacity by modified activated carbon immobilized cells.
Background
Chromium is an element that is relatively high in content and widely distributed in natural resources. Chromium is mainly introduced into various environments, such as surface water, groundwater, seawater, soil, rock and air, in two ways, naturally occurring and artificially active, and is present in the crust in different concentrations and in different combinations. Chromium is one of the most commonly used metal pollutants, one of the 129 serious pollution pests recognized by the united states EPA, and one of the three internationally recognized carcinogens. In recent years, chromium pollution events are in multiple situations in Hunan, yunnan and the like in China, and serious social influence is caused. Therefore, the present invention has been made by the following description, and it is an object of the present invention to provide a novel method for manufacturing a semiconductor device. Various disadvantages exist in the physicochemical treatment of chromium pollution. Therefore, researchers at home and abroad begin to transfer research targets to a novel method, namely a bioremediation method. The bioremediation method converts Cr (VI) with high toxicity and strong mobility into Cr (III) with low toxicity and weak mobility through the adsorption and enrichment of microorganisms on Cr (VI) or the metabolism of microorganisms, and finally achieves the aim of reducing Cr (VI) ions contained in the environment. The bioremediation method for treating heavy metal pollution is not applied to practice until the last 80 th century. The bioremediation method has the advantages of easy control, small investment, high efficiency, low cost and no secondary pollution, thus having good application prospect in environmental repair. However, the microbial remediation has the defects of poor microbial adaptability, susceptibility to external environment, low efficiency and the like, so that the wide application of the microbial remediation is limited. In order to improve the tolerance of microorganisms and increase the metal reduction efficiency of strains, a large number of researchers have explored various methods for reinforcing microorganisms to repair chromium pollution from various directions such as physics, chemistry, biology and the like.
The microorganism immobilization technology has wide application and good results in the aspect of treating metal and organic pollution in refractory and toxic materials. Compared with the traditional biological treatment technology, the method adopts the microorganism immobilization technology and combines the adsorption characteristic of the activated carbon to reduce hexavalent chromium pollution in the water body, thereby having obvious superiority. According to the invention, the reduction efficiency of hexavalent chromium can be remarkably increased by adding the modified activated carbon, so that the achievement of the invention has good practicability.
Disclosure of Invention
The invention aims to improve the efficiency of hexavalent chromium reduction by microorganisms, and discloses a method for improving the reduction capacity of bacterial chromium by modified activated carbon immobilized cells.
The technical scheme of the invention is summarized as follows:
a method for increasing the reducing capacity of bacterial chromium by modified activated carbon immobilized cells, comprising the following steps:
1. crushing active carbon into particles with the size of more than 200 meshes;
2.5 g of activated carbon was added to a mixture containing 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% (w/v) PEI (polyetherimide) methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% (w/v) glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
3. the strain is derived from laboratory screening separation, the selection culture medium is an improved liquid LB culture medium, and the formula is prepared: peptone 10 g.L -1 Sodium chloride 5 g.L -1 Yeast extract 5 g.L -1 Dipotassium hydrogen phosphate 0.05 g.L -1 Magnesium sulfate heptahydrate 0.2 g.L -1 If the solid LB culture medium is adopted, agar is added with 1.5 to 2.0 percent, the pH value is 8.5 to 9.0, and sterilization is carried out;
4. culturing micrococcus obtained by screening and separating in LB-containing culture medium under shaking flask condition of initial pH 8.0-9.0 at 30deg.C and shaking table rotation speed of 170rpm;
5. the conditions of the immobilized pellet are: 3g of sodium alginate, 4g of calcium chloride and 0.1-0.5g of modified activated carbon, dissolving sodium alginate and modified activated carbon in 90ml of distilled water, and sterilizing;
6. filtering and centrifugally collecting the micrococcus cultured in the step (4), and obtaining the micrococcus according to the OD of the inoculation amount 600 The value is 1.5, 10ml of sterile water is prepared and evenly mixed with the mixed solution in the step (4), the mixed solution is slowly dripped into a calcium chloride solution with the mass concentration of 4% w/v, after crosslinking for four hours, the mixed solution is added into LB liquid medium containing 120mg/L Cr (VI), and the mixed solution is subjected to shaking culture at room temperature for 84 hours.
7. Preparing a dibenzoyl dihydrazide solution, dissolving 0.2g of dibenzoyl dihydrazide in 50ml of acetone, transferring to a 100ml volumetric flask, diluting with water to scale mark, shaking, and slowly adding 12.5ml of concentrated H into the refrigerating solution 2 SO 4 And 12.5ml of concentrated phosphoric acid, stored in a dark place.
In the step (3), 10g of a sample is taken and added into 90ml of modified sterilized liquid LB medium, dilution is carried out by 10 times gradient, 0.2ml of each sample is absorbed, the sample is coated on 200mg/L Cr (VI) containing solid modified LB medium, a coating rod is used for uniformly coating bacterial liquid, the bacterial liquid is cultured in a constant temperature incubator at 30 ℃ for 24 hours, bacterial colonies grow out, and the separated bacterial strains are identified as micrococcus.
Measuring the concentration of hexavalent chromium in the sample by adopting a dibenzoyl dihydrazide spectrophotometry for the dibenzoyl dihydrazide solution obtained in the step (7), wherein the calculation formula of the concentration of Cr (VI) is y=0.6031x+0.0065, and the correlation coefficient R of a standard curve 2 ＝0.0997。
The modified activated carbon material has various advantages for removing pollutants, the structure (specific surface area, porosity and the like) or the performance of the modified activated carbon material is optimized, and the repairing effect on heavy metal pollution is better. The method can obviously improve the reduction efficiency of micrococcus to hexavalent chromium in water by improving the activated carbon and controlling the dosage (0.3 g) of the activated carbon, and the reduction rate is improved by 13.4%.
Drawings
FIG. 1 is a graph of hexavalent chromium concentration versus time for example 1;
FIG. 2 is a graph of hexavalent chromium concentration versus time for example 2;
FIG. 3 is a graph of hexavalent chromium concentration versus time for example 3;
FIG. 4 is a graph of hexavalent chromium concentration versus time for example 4;
FIG. 5 is a graph of hexavalent chromium concentration versus time for example 5;
FIG. 6 is a view showing the immobilized beads without the modified activated carbon added;
FIG. 7 is a view showing the addition of modified activated carbon immobilized beads.
Detailed Description
The following specific examples or embodiments are intended to further illustrate the invention, and are not intended to be limiting.
Example 1
The method in this embodiment mainly comprises the following steps:
(1) Crushing active carbon into particles with the particle size of more than 200 meshes;
(2) 5g of activated carbon was added to a mixture containing 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% (w/v) PEI (polyetherimide)/methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% (w/v) glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
(3) Preparing LB liquid culture medium, shake culturing micrococcus to logarithmic phase, and taking OD 600 Bacterial liquid with a value of about 1.5 is collected by centrifugation and prepared into 10ml bacterial suspension by using sterile water;
(4) Weighing 3g of sodium alginate and 0.1g of modified activated carbon, adding 90ml of distilled water system, sterilizing, uniformly mixing 10ml of bacterial suspension in the step (2) with the bacterial suspension, uniformly dripping the bacterial suspension into 4% w/v calcium chloride solution, standing for four hours at room temperature, pouring the washed pellets into LB liquid medium containing 120mg/L Cr (VI), and culturing for 84 hours at the temperature of 30 ℃ and the rotation speed of a shaking table of 170rpm under the condition that the initial pH value is 8.0;
conclusion: as shown in FIG. 1, the hexavalent chromium reduction ratio added with 0.1g of the modified activated carbon was 72.5%, the hexavalent chromium reduction ratio without adding the modified activated carbon was 64.1%, and the hexavalent chromium reduction ratio was improved by 8.4%.
Example 2
The method in this embodiment mainly comprises the following steps:
(1) Crushing active carbon into particles with the particle size of more than 200 meshes;
(2) 5g of activated carbon was added to a mixture containing 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% (w/v) PEI (polyetherimide)/methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% (w/v) glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
(3) Preparing LB liquid culture medium, shake culturing micrococcus to logarithmic phase, and taking OD 600 Bacterial liquid with a value of about 1.5 is collected by centrifugation and prepared into 10ml bacterial suspension by using sterile water;
(4) Weighing 3g of sodium alginate and 0.2g of modified activated carbon, adding 90ml of distilled water system, sterilizing, uniformly mixing 10ml of bacterial suspension in the step (2) with the bacterial suspension, uniformly dripping the bacterial suspension into 4% w/v calcium chloride solution, standing for four hours at room temperature, pouring the washed pellets into LB liquid medium containing 120mg/L Cr (VI), and culturing for 84 hours at the temperature of 30 ℃ and the rotation speed of a shaking table of 170rpm under the condition that the initial pH value is 8.0;
conclusion: as shown in FIG. 2, the hexavalent chromium reduction ratio was 73.3% with the addition of 0.2g of the modified activated carbon, 64.1% without the addition of the modified activated carbon, and a 9.2% improvement in the hexavalent chromium reduction ratio.
Example 3
The method in this embodiment mainly comprises the following steps:
(1) Crushing active carbon into particles with the particle size of more than 200 meshes;
(2) 5g of activated carbon was added to a mixture containing 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% (w/v) PEI (polyetherimide)/methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% (w/v) glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
(3) Preparing LB liquid culture medium, shake culturing micrococcus to logarithmic phase, and taking OD 600 Bacterial liquid with a value of about 1.5 is collected by centrifugation and prepared into 10ml bacterial suspension by using sterile water;
(4) Weighing 3g of sodium alginate and 0.3g of modified activated carbon, adding 90ml of distilled water system, sterilizing, uniformly mixing 10ml of bacterial suspension in the step (2) with the bacterial suspension, uniformly dripping the bacterial suspension into 4% w/v calcium chloride solution, standing for four hours at room temperature, pouring the washed pellets into LB liquid medium containing 120mg/L Cr (VI), and culturing for 84 hours at the temperature of 30 ℃ and the rotation speed of a shaking table of 170rpm under the condition that the initial pH value is 8.0;
conclusion: as shown in FIG. 3, the hexavalent chromium reduction ratio added with 0.3g of the modified activated carbon was 77.5%, the hexavalent chromium reduction ratio without adding the modified activated carbon was 64.1%, and the hexavalent chromium reduction ratio was improved by 13.4%.
Example 4
The method in this embodiment mainly comprises the following steps:
(1) Crushing active carbon into particles with the particle size of more than 200 meshes;
(2) 5g of activated carbon was added to a mixture containing 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% (w/v) PEI (polyetherimide)/methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% (w/v) glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
(3) Preparing an LB liquid culture medium, culturing micrococcus in a shake flask to a logarithmic growth phase, taking bacterial liquid with an OD600 value of about 1.5, centrifugally collecting, and preparing 10ml bacterial suspension by using sterile water;
(4) Weighing 3g of sodium alginate and 0.4g of modified activated carbon, adding 90ml of distilled water system, sterilizing, uniformly mixing 10ml of bacterial suspension in the step (2) with the bacterial suspension, uniformly dripping the bacterial suspension into 4% w/v calcium chloride solution, standing for four hours at room temperature, pouring the washed pellets into LB liquid medium containing 120mg/L Cr (VI), and culturing for 84 hours at the temperature of 30 ℃ and the rotation speed of a shaking table of 170rpm under the condition that the initial pH value is 8.0;
conclusion: as shown in FIG. 4, the hexavalent chromium reduction ratio was 75.83% with the addition of 0.4g of the modified activated carbon, 64.1% without the addition of the modified activated carbon, and an improvement of 11.73% was obtained.
Example 5
The method in this embodiment mainly comprises the following steps:
(1) Crushing active carbon into particles with the particle size of more than 200 meshes;
(2) 5g of living organismCharcoal is added into 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% (w/v) PEI (polyetherimide)/methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% (w/v) glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
(3) Preparing LB liquid culture medium, shake culturing micrococcus to logarithmic phase, and taking OD 600 Bacterial liquid with a value of about 1.5 is collected by centrifugation and prepared into 10ml bacterial suspension by using sterile water;
(4) Weighing 3g of sodium alginate and 0.5g of modified activated carbon, adding 90ml of distilled water system, sterilizing, uniformly mixing 10ml of bacterial suspension in the step (2) with the bacterial suspension, uniformly dripping the bacterial suspension into 4% w/v calcium chloride solution, standing for four hours at room temperature, pouring the washed pellets into LB liquid medium containing 120mg/L Cr (VI), and culturing for 84 hours at the temperature of 30 ℃ and the rotation speed of a shaking table of 170rpm under the condition that the initial pH value is 8.0;
conclusion: as shown in FIG. 5, the hexavalent chromium reduction ratio was 75% by adding 0.5g of the modified activated carbon, 64.1% by not adding the modified activated carbon, and 10.9% by increasing the hexavalent chromium reduction ratio.
Claims (2)
1. A method for enhancing the chromium reduction capability of bacteria by modified activated carbon immobilized cells, which is characterized by comprising the following steps:
(1) Crushing active carbon into particles with the particle size of more than 200 meshes;
(2) 5g of activated carbon was added to a mixture containing 50ml of 70% HNO 3 Stirring at 170rpm for 1h at room temperature to remove impurities in the activated carbon, washing with deionized water to neutrality, and drying thoroughly; adding the dried activated carbon into 100ml of 10% w/v PEI/methanol solution, stirring at 170rpm for 24 hours at room temperature, immediately transferring the activated carbon into 200ml of 1% w/v glutaraldehyde solution for crosslinking, and finally washing with deionized water to obtain modified activated carbon;
(3) Configuration ofLB liquid culture medium, shake flask culture micrococcus to logarithmic phase, OD 600 Bacterial liquid with a value of 1.5 is collected by centrifugation and prepared into 10ml bacterial suspension by sterile water;
(4) Weighing 3g of sodium alginate, 0.1,0.2,0.3,0.4 or 0.5g of modified activated carbon, adding a 90ml distilled water system, sterilizing, uniformly mixing 10ml bacterial suspension with the sodium alginate, uniformly dripping the bacterial suspension into 4% w/v calcium chloride solution, standing for four hours at room temperature, pouring the washed pellets into an LB liquid medium containing 120mg/L Cr (VI), wherein the culture condition is that the initial pH is 8.0, the temperature is 30 ℃, the rotation speed of a shaking table is 170rpm, and culturing is 84h; preparing a dibenzoyl dihydrazide solution, dissolving 0.2g of dibenzoyl dihydrazide in 50ml acetone, transferring to a 100ml volumetric flask, diluting with water to scale mark, shaking, and sequentially adding 12.5ml concentrated H into the refrigerating solution 2 SO 4 And 12.5. 12.5ml concentrated phosphoric acid, stored in the dark.
2. The method for enhancing bacterial chromium reduction capacity by modified activated carbon immobilized cells according to claim 1, wherein the concentration of hexavalent chromium in the sample is determined by using a dibenzoyl dihydrazide spectrophotometry on the dibenzoyl dihydrazide solution obtained in the step (4), the calculated formula of the concentration of Cr (VI) is y=0.6031x+0.0065, and the correlation coefficient R of a standard curve 2 =0.0997。
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| CN114686377B (en) * | 2020-12-31 | 2023-07-04 | 中国石油化工股份有限公司 | Preparation and preservation method of nitrifying bacteria agent |
| CN113149230B (en) * | 2021-02-26 | 2022-04-19 | 中南大学 | Treatment method for reducing hexavalent chromium by wood charcoal electron transfer enhanced microorganisms |
| CN115286119B (en) * | 2022-07-20 | 2023-09-12 | 西南科技大学 | Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5681739A (en) * | 1995-05-25 | 1997-10-28 | Lockheed Idaho Technologies Company | Method for in situ or ex situ bioremediation of hexavalent chromium contaminated soils and/or groundwater |
| CN1834226A (en) * | 2005-03-17 | 2006-09-20 | 中南大学 | One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method |
| JP2007020540A (en) * | 2005-07-21 | 2007-02-01 | Tokai Kinzoku Kogyo Kk | New microorganism having hexavalent chromium-reducing ability and method for cleaning environment therewith |
| WO2007020588A1 (en) * | 2005-08-12 | 2007-02-22 | Universidade Do Minho | Biosorption system produced from biofilms supported on faujasite (fau) zeolite, process obtaining it and its usage for removal of hexavalent chromium (cr (vi)) |
| CN101058453A (en) * | 2007-04-12 | 2007-10-24 | 同济大学 | Method of eliminating hexavalent chromium in sewage by using active sludge microorganism |
| CN104386831A (en) * | 2014-12-03 | 2015-03-04 | 西南科技大学 | Method of treating hexavalent chromium wastewater by immobilized microorganisms |
| CN106636057A (en) * | 2016-11-30 | 2017-05-10 | 湖南大学 | Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof |
| CN106834269A (en) * | 2017-03-21 | 2017-06-13 | 青岛农业大学 | A kind of immobilized microspheres of PAEs degradation bacterias and its preparation method and application |
| CN108018229A (en) * | 2017-11-09 | 2018-05-11 | 中南大学 | A kind of hexavalent chrome reduction immobilized microbial inoculum and preparation method |
| CN109321498A (en) * | 2018-10-16 | 2019-02-12 | 苏州市农业科学院 | A kind of screening technique of resistance to huge sum of money microorganism belonging to genus |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IES86631B2 (en) * | 2014-11-18 | 2016-02-24 | Microgen Biotech Ltd | A process for the remediation of hydrocarbons and/or heavy metal contaminated soil/sludge sediment |
| CN105255782B (en) * | 2015-11-16 | 2019-04-12 | 四川省兰月科技有限公司 | There is fiber bacterium and the purposes of reducing power to Cr VI |
| CN105268738A (en) * | 2015-11-25 | 2016-01-27 | 武汉科技大学 | Method for stabilizing chromium pollution soil in chromium salt factory through nitric acid modified activated carbon |
| CN106833674B (en) * | 2017-02-09 | 2020-08-14 | 东莞市环境科学研究所 | Preparation method of heavy metal contaminated soil remediation agent |
| CN109174026B (en) * | 2018-09-18 | 2022-01-18 | 浙江海洋大学 | Composite microalgae biological adsorbent microsphere and method for adsorbing chromium in wastewater by using composite microalgae biological adsorbent microsphere |
-
2019
- 2019-06-17 CN CN201910519672.6A patent/CN110241111B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5681739A (en) * | 1995-05-25 | 1997-10-28 | Lockheed Idaho Technologies Company | Method for in situ or ex situ bioremediation of hexavalent chromium contaminated soils and/or groundwater |
| CN1834226A (en) * | 2005-03-17 | 2006-09-20 | 中南大学 | One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method |
| JP2007020540A (en) * | 2005-07-21 | 2007-02-01 | Tokai Kinzoku Kogyo Kk | New microorganism having hexavalent chromium-reducing ability and method for cleaning environment therewith |
| WO2007020588A1 (en) * | 2005-08-12 | 2007-02-22 | Universidade Do Minho | Biosorption system produced from biofilms supported on faujasite (fau) zeolite, process obtaining it and its usage for removal of hexavalent chromium (cr (vi)) |
| CN101058453A (en) * | 2007-04-12 | 2007-10-24 | 同济大学 | Method of eliminating hexavalent chromium in sewage by using active sludge microorganism |
| CN104386831A (en) * | 2014-12-03 | 2015-03-04 | 西南科技大学 | Method of treating hexavalent chromium wastewater by immobilized microorganisms |
| CN106636057A (en) * | 2016-11-30 | 2017-05-10 | 湖南大学 | Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof |
| CN106834269A (en) * | 2017-03-21 | 2017-06-13 | 青岛农业大学 | A kind of immobilized microspheres of PAEs degradation bacterias and its preparation method and application |
| CN108018229A (en) * | 2017-11-09 | 2018-05-11 | 中南大学 | A kind of hexavalent chrome reduction immobilized microbial inoculum and preparation method |
| CN109321498A (en) * | 2018-10-16 | 2019-02-12 | 苏州市农业科学院 | A kind of screening technique of resistance to huge sum of money microorganism belonging to genus |
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