CN103626304A - Application of cordyceps sinensis fermentation mycelium in heavy metal wastewater treatment, and treatment method - Google Patents

Application of cordyceps sinensis fermentation mycelium in heavy metal wastewater treatment, and treatment method Download PDF

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Publication number
CN103626304A
CN103626304A CN201310657653.2A CN201310657653A CN103626304A CN 103626304 A CN103626304 A CN 103626304A CN 201310657653 A CN201310657653 A CN 201310657653A CN 103626304 A CN103626304 A CN 103626304A
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heavy metal
cordyceps
mycelia
base
cordyceps sinensis
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CN201310657653.2A
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CN103626304B (en
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杨波
胡征
刘志刚
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Hubei University of Technology
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Hubei University of Technology
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Abstract

The invention discloses an application of a cordyceps sinensis fermentation mycelium in heavy metal wastewater treatment, and a treatment method, and belongs to the field of environmental protection. The method comprises the steps that the cordyceps sinensis fermentation mycelium is directly put into heavy metal wastewater, uniformly mixed, shaken and absorbed, and then heavy metal in the wastewater can be removed. The cordyceps sinensis fermentation mycelium is obtained by cultivating cordyceps sinensis in a fermentation medium, obtaining fermentation liquid and performing centrifugation, wherein the fermentation medium comprises the following components by mass volume ratio based on the total volume of the fermentation liquid: 1-4% of soybean flour, 2-6% of corn flour, 2-6% of sucrose, 0-0.5% of yeast powder, 0.1-0.5% of KH2PO4, and 0.01-0.1% of MgSO4. According to the application and the treatment method, the cordyceps sinensis fermentation mycelium is used for heavy metal wastewater treatment, so that the method is low in production cost, environment-friendly, simple in preparation technology, high in heavy metal removal rate and wide in application prospect, and is particularly suitable for treating wastewater containing low-concentration heavy metal.

Description

Application and the treatment process of Base in Mycelia of Cordyceps in heavy metal containing wastewater treatment
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Technical field
The invention belongs to field of environment protection, be specifically related to application and the treatment process of a kind of Base in Mycelia of Cordyceps in heavy metal containing wastewater treatment.
Background technology
Heavy metal typically refers to proportion at more than 5 metals.Heavy metal is distributed widely in atmospheric sphere, litre geosphere, biosphere and hydrosphere.Under normal circumstances, heavy metal nature background concentration can not reach harmful degree.But along with large-scale commercial production and blowdown and applying pesticides on a large scale, poisonous and harmful metal, as lead, cadmium, mercury, arsenic, chromium, antimony etc. enter atmosphere, water body and soil, causes the heavy metal contamination of environment.Heavy metal environmental pollutant form the property heavy metal contamination of food source by food chain.
Heavy metal embodies a concentrated reflection of in the hard degradation of heavy metal and is difficult to find the hazardness of human body.Generally organic compound can, by nature self physics, chemistry or biological purification, reduce or releasing hazardous property; And heavy metal has enriching, be difficult to degrade in environment, by thousands of hundred times of enrichments under the biological magnification of food chain, then enter human body, be difficult to Natural excrement or thoroughly remove, to the harm of human body, be generally irreversible throughout one's life.Therefore, relevant scholar is actively seeking minimizing or is removing the method to the heavy metal of severe environmental pollution both at home and abroad.
The traditional method of removing heavy metal mainly contains chemical precipitation method, ion exchange method, electrolytic process and membrane separation technique etc.These treatment processs have obtained good effect to a certain extent, but during these secondary pollutions of method ubiquity, the particularly heavy metal concentration in water lower (being less than 100 mg/L), not only clearance is low, and expense is high.
Biosorption process is a kind of method of wastewater treatment of novelty, and this method is utilized the metal ion in organism or derivatives thereof planar water, reaches the object of removal heavy metal.At present, biological adsorption agent mainly comprises bacterium, fungi, algae and agricultural wastes, and its adsorption mechanism mainly comprises ion-exchange mechanism, surface engagement mechanism, redox and inorganic microdeposit mechanism and enzymatic mechanism.The advantages such as this method has efficiently, high specific, non-secondary pollution, sorbing material wide material sources.Therefore utilize biomaterial accumulation heavy metal to become the research and development focus of current such wastewater processing technology.But these natural biomaterials adsorb limited in one's ability with heavy-metal ion removal in general, need to carry out modification to it, just can give full play to the ability that it processes heavy metal ion.So just increase to a certain extent the cost of processing, made complex treatment process, be unfavorable for large scale application.And method of modifying mostly is chemical method, need to use the chemical reagent such as strong acid and strong base, from and introduced chemical pollution.
Summary of the invention
Primary and foremost purpose of the present invention is that the shortcoming that overcomes prior art, with not enough, provides the application of a kind of Base in Mycelia of Cordyceps in heavy metal containing wastewater treatment.
Another object of the present invention is to provide a kind of method of processing heavy metal wastewater thereby by Base in Mycelia of Cordyceps.
Object of the present invention is achieved through the following technical solutions:
The application of Base in Mycelia of Cordyceps in heavy metal containing wastewater treatment.
A method of processing heavy metal wastewater thereby by Base in Mycelia of Cordyceps, comprises the following steps: Base in Mycelia of Cordyceps is directly put in heavy metal wastewater thereby, mixed, concussion absorption.
The concentration of described heavy metal wastewater thereby is preferably 5～500 mg/L.
The dosage of described Base in Mycelia of Cordyceps is preferably 100～1000 mg/100 mL(M dry weight/ V), more excellent is 100～300 mg/100 mL.
The condition optimization of described concussion absorption is: temperature is 25～30 ℃; Concussion rotating speed is 120～220 rpm; Adsorption time is 2～12 hours, and more excellent is 6～8 hours.
The application of Base in Mycelia of Cordyceps in adsorbent for heavy metal.
A kind of heavy metal ion microbial adsorbent is Base in Mycelia of Cordyceps.
An adsorbent for heavy metal, comprises Base in Mycelia of Cordyceps.
Above-mentioned Base in Mycelia of Cordyceps preferably obtains by the preparation method who comprises the steps:
(1) by Cordyceps sinensis ( cordyceps sinensis) in fermention medium, carry out fermentation culture, obtain fermented liquid;
(2) fermented liquid is centrifugal, to obtain Base in Mycelia of Cordyceps precipitation.
The formula optimization of described fermention medium is: in the cumulative volume of fermented liquid, described fermention medium contains following component (mass volume ratio): soyflour 1～4%, and more excellent is 2%; Semen Maydis powder 2～6%, more excellent is 4%; Sucrose 2～6%, more excellent is 4%; Yeast powder 0～0.5%, more excellent is 0.3%; KH 2pO 40.1～0.5%, more excellent is 0.2%; MgSO 40.01～0.1%, more excellent is 0.04%; The pH of described fermention medium is 5～7, and more excellent is 6.
The condition optimization of described fermentation culture is: 25～30 ℃ of temperature, and more excellent is 28 ℃; Shaking speed 100～220 rpm, more excellent is 150 rpm; Liquid amount 30～80 mL/250 mL, more excellent is 40 mL/250 mL; Incubation time 48～120 hours, more excellent is 72 hours.
Compared with prior art, the present invention has the following advantages and effect:
(1) the present invention is usingd Base in Mycelia of Cordyceps as heavy metal ion microbial adsorbent, it have desirable adsorption effect without any physics or chemical modification processing, so just greatly reduce the complicacy of sorbent material production technique and the cost of application, make it be easy to promote.
(2) microbial adsorbent provided by the present invention (Base in Mycelia of Cordyceps) to containing the waste water of low-concentration heavy metal (in waste water cumulative volume, its heavy metal content is less than 10 mg/L) adsorption effect very for desirable, solved traditional biological sorbent material to the undesirable difficult problem of water treatment effect containing low-concentration heavy metal.
(3) Base in Mycelia of Cordyceps as microbial adsorbent provided by the invention can be take cheap agricultural byproducts and carried out large scale fermentation production as main raw material, leavening temperature approaches room temperature, save energy, fermentation biomass is high (in the situation that of common shake flask fermentation, in fermented liquid cumulative volume, it can reach 35 g/L(M dry weight/ V) above biomass, if adopt fermentor tank to produce, output may be higher), low production cost.
(4) Base in Mycelia of Cordyceps, density is larger, is easy to sedimentation, is easy to results.
(5) production process of Base in Mycelia of Cordyceps is environmentally friendly: on the one hand, Cordyceps sinensis itself is exactly herbal medicine, nontoxic, and its fermentation mycelium production process does not cause biological pollution; On the other hand, the filtrate of fermented liquid is contained a large amount of Cordyceps exocellular polysaccharides, also there is higher recycling to be worth (such as can be used for making cordyceps tea etc.), and the more microbial adsorbent of studying is at present as head mold, cereuisiae fermentum, the filtrate of its fermented liquid all belongs to waste liquid, and directly discharge can cause environmental pollution.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated; But the present invention is not limited to these embodiment.
The conventional bacterial classification that the microorganism of using in following examples can obtain for the public.Obviously, those skilled in the art also can verify technical scheme of the present invention with other Cordyceps strain.Described technical scheme, if not otherwise specified, is routine techniques scheme, and agents useful for same is this area common agents.
Embodiment 1
By fresh Cordyceps sinensis ( cordyceps sinensis) (buy in Chinese agriculture microbial strains preservation administrative center (ACCC), deposit number is 50560) be inoculated in fermention medium and in shaking flask, carry out fermentation culture, obtains fermented liquid; The fermentation mycelium precipitation obtaining after fermented liquid is centrifugal with 4000 rpm is microbial adsorbent.Described fermentative medium formula is: in the cumulative volume of fermented liquid, described fermention medium contains following component (mass volume ratio): soyflour 2%, Semen Maydis powder 4%, sucrose 4%, yeast powder 0.3%, KH 2pO 40.2%, MgSO 40.04%; The pH of fermention medium is 6.Fermentation culture conditions is: 28 ℃ of temperature, shaking speed 150 rpm, liquid amount 50 mL/250 mL, incubation time 72 h.In the cumulative volume of fermented liquid, the output of gained microbial adsorbent is (mass volume ratio) 35 g/L(M dry weight/ V).
By the fermentation mycelium of above-mentioned fresh preparation, directly put in the waste water of heavy metal Cr (VI), mix concussion absorption.In the cumulative volume of the waste water containing heavy metal, wherein, thalline dosage is 100 mg/100 mL (M dry weight/ V), Cr(VI in waste water) concentration is 10 mg/L, and adsorption temp is 28 ℃, and concussion rotating speed is 180 rpm, adsorption time 8 h, and clearance is 98.1%.
The calculation formula of above-mentioned clearance is: clearance (%)=(C 0-C)/C 0* 100%.
In formula: C 0for Cr(VI) initial concentration (mg/ L), C is remaining Cr(VI) concentration is adsorption equilibrium concentration (mg/L).
Above-mentioned containing the Cr(VI in the waste water of heavy metal) detection method of ionic concn is diphenyl carbazide spectrophotometry, concrete grammar is referring to State Environmental Protection Administration. water and tailing water analysis detection method (the 4th edition). Beijing: Chinese environmental press.
Embodiment 2
By fresh Cordyceps sinensis ( cordyceps sinensis) (buy in Chinese agriculture microbial strains preservation administrative center (ACCC), deposit number is 50560) be inoculated in fermention medium and in shaking flask, carry out fermentation culture, obtains fermented liquid; The fermentation mycelium precipitation obtaining after fermented liquid is centrifugal with 4000 rpm is microbial adsorbent.Described fermentative medium formula is: in the cumulative volume of fermented liquid, described fermention medium contains following component (mass volume ratio): soyflour 2%, Semen Maydis powder 4%, sucrose 6%, yeast powder 0.1%, KH 2pO 40.2%, MgSO 40.04%; The pH of fermention medium is 6.Culture condition is: 28 ℃ of temperature, shaking speed 150 rpm, liquid amount 50 mL/250 mL, incubation time 96 h.In the cumulative volume of fermented liquid, the output of gained microbial adsorbent is (mass volume ratio) 38 g/L(M dry weight/ V).
By the fermentation mycelium of above-mentioned fresh preparation, directly throw in the waste water of heavy metal Cr (VI), mix concussion absorption.In the cumulative volume of the waste water containing heavy metal, wherein, thalline dosage is 200 mg/100 mL (M dry weight/ V), Cr(VI in waste water) concentration is 50 mg/L, and adsorption temp is 28 ℃, and concussion rotating speed is 180 rpm, adsorption time 6 h, and clearance is 93.6%.
The calculation formula of above-mentioned clearance is: clearance (%)=(C 0-C)/C 0* 100%.
In formula: C 0for Cr(VI) initial concentration (mg/ L), C is remaining Cr(VI) concentration is adsorption equilibrium concentration (mg/L).
Above-mentioned containing the Cr(VI in the waste water of heavy metal) detection method of ionic concn is diphenyl carbazide spectrophotometry, concrete grammar is referring to State Environmental Protection Administration. water and tailing water analysis detection method (the 4th edition). Beijing: Chinese environmental press.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.

Claims (9)

1. the application of Base in Mycelia of Cordyceps in heavy metal containing wastewater treatment.
2. by Base in Mycelia of Cordyceps, process a method for heavy metal wastewater thereby, it is characterized in that comprising the following steps: Base in Mycelia of Cordyceps is directly put in heavy metal wastewater thereby, mixed, concussion absorption.
3. the method for processing heavy metal wastewater thereby by Base in Mycelia of Cordyceps according to claim 2, is characterized in that: the concentration of described heavy metal wastewater thereby is 5～500 mg/L.
4. the method for processing heavy metal wastewater thereby by Base in Mycelia of Cordyceps according to claim 2, is characterized in that: the dosage of described Base in Mycelia of Cordyceps is 100～1000 mg/100 mL.
5. the method for processing heavy metal wastewater thereby by Base in Mycelia of Cordyceps according to claim 2, is characterized in that: the condition of described concussion absorption is: temperature is 25～30 ℃, and concussion rotating speed is 120～220 rpm, and adsorption time is 2～12 hours.
6. the application of Base in Mycelia of Cordyceps in adsorbent for heavy metal.
7. a heavy metal ion microbial adsorbent, is characterized in that: be Base in Mycelia of Cordyceps.
8. an adsorbent for heavy metal, is characterized in that: comprise Base in Mycelia of Cordyceps.
9. according to method, microbial adsorbent claimed in claim 7 or sorbent material claimed in claim 8 described in the application described in claim 1 or 6, claim 2～5 any one, it is characterized in that: described Base in Mycelia of Cordyceps obtains by the preparation method who comprises the steps:
(1) Cordyceps sinensis is carried out in fermention medium to fermentation culture, obtain fermented liquid;
(2) fermented liquid is centrifugal, to obtain Base in Mycelia of Cordyceps precipitation;
In the cumulative volume of fermented liquid, described fermention medium contains following mass volume ratio component: soyflour 1～4%, Semen Maydis powder 2～6%, sucrose 2～6%, yeast powder 0～0.5%, KH 2pO 40.1～0.5%, MgSO 40.01～0.1%; The pH of described fermention medium is 5～7;
The condition of described fermentation culture is: 25～30 ℃ of temperature, shaking speed 100～220 rpm, liquid amount 30～80 mL/250 mL, incubation time 48～120 hours.
CN201310657653.2A 2013-12-09 2013-12-09 A kind of by the method for Base in Mycelia of Cordyceps process containing Cr (VI) waste water Expired - Fee Related CN103626304B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105016477A (en) * 2014-04-16 2015-11-04 上海理工大学 Technology for processing soybean wastewater by utilization of filamentous fungi
CN111252843A (en) * 2020-01-20 2020-06-09 中南林业科技大学 Hypha filter membrane device for selectively treating complex wastewater and treatment process

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CN1920000A (en) * 2006-09-04 2007-02-28 翟春涛 Producing technique of Jiuzhou caterpillar fungus containing rich chromium and culture medium

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CN1920000A (en) * 2006-09-04 2007-02-28 翟春涛 Producing technique of Jiuzhou caterpillar fungus containing rich chromium and culture medium

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105016477A (en) * 2014-04-16 2015-11-04 上海理工大学 Technology for processing soybean wastewater by utilization of filamentous fungi
CN111252843A (en) * 2020-01-20 2020-06-09 中南林业科技大学 Hypha filter membrane device for selectively treating complex wastewater and treatment process
CN111252843B (en) * 2020-01-20 2022-08-16 中南林业科技大学 Hypha filter membrane device for selectively treating complex wastewater and treatment process

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