CN107262495B - A method of refuse odor is administered using composite bacteria agent - Google Patents

A method of refuse odor is administered using composite bacteria agent Download PDF

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CN107262495B
CN107262495B CN201710466841.5A CN201710466841A CN107262495B CN 107262495 B CN107262495 B CN 107262495B CN 201710466841 A CN201710466841 A CN 201710466841A CN 107262495 B CN107262495 B CN 107262495B
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fermentation liquid
composite bacteria
liquid
bacteria agent
aspergillus niger
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CN107262495A (en
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蒋文垚
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Quanzhou Modern Biotechnology Co., Ltd
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Hangzhou Fuyang Youxin Technology Co Ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09BDISPOSAL OF SOLID WASTE
    • B09B3/00Destroying solid waste or transforming solid waste into something useful or harmless
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The invention belongs to environmental technology fields, disclose a kind of method for administering refuse odor using composite bacteria agent comprising composite bacteria agent: 1) being added in 30-35 DEG C of the water of 10 times of weight by following steps, and 200rpm stirs 10min, obtains dilution;2) rubbish is paved into 40cm thickness, is then sprayed according to every square metre of additive amount using 90g dilution, the processing time is 72h.The present invention handles refuse odor using composite bacteria agent, can be effectively removed the gases such as ammonia, hydrogen sulfide, good deodorization effect, operation is simple and feasible.

Description

A method of refuse odor is administered using composite bacteria agent
Technical field
The invention belongs to environmental technology fields, and in particular to a method of refuse odor is administered using composite bacteria agent.
Background technique
With the continuous development of urbanization process, people are increasingly toward urban agglomerations, and domestic waste is also with annual 10% Above speed increase.The generation of domestic waste and its bring environmental pollution have attracted people's attention, especially In down town garbage collection points and municipal refuse terminal, the stench that house refuse is caused is distributed, and mosquitos and flies multiplies, pest and disease damage The problems such as propagation, affects to quality of residents'life and local environment.Domestic waste collect and The management of transfer is lack of standardization, can seriously destroy the ecology in city, cause people's psychology unhappy.
Common method has absorption method, combustion method, energetic ion method and bioanalysis both at home and abroad at present.Absorption method includes acid The washing of alkali liquid medicine and activated carbon adsorption, the former needs to consume a large amount of chemical agents, the latter Ze Yi blocking, and replacement frequently, is safeguarded It is costly;Combustion method is suitable for the organic exhaust gas of high concentration, high heating value, seldom applies in general deodorization engineering;High energy The ozone direct emission that ion method generates, it is possible to Environmental security can be endangered, and its ion generated has strong oxidation, it is right Equipment has corrosiveness, and also has certain injury to human body;Bioanalysis is one kind by being added into environment from nature The beneficial microbe colony of middle screening or the biological inoculum generated by genetic recombination, it is raw as purpose environment to improve air environment Object technology, general cost is lower, but is difficult to find the microbial inoculum that compatibility is reasonable, effect is good, and deodorizing effect is not ideal enough, is not achieved The requirement of people.Based on the above issues, Chinese patent technology " solid refuse cleaning bacterial activity agent brown alga deodorant " is open A kind of biological deodorant, deodorizing effect is preferable, but there are complicated component, and with high costs and preparation process is complicated etc. lacks It falls into.
Summary of the invention
In order to overcome the defect of biologic deodorization method in the prior art, administered the present invention provides a kind of using composite bacteria agent The method of refuse odor.
The present invention is achieved by the following technical scheme:
A method of refuse odor is administered using composite bacteria agent comprising following steps:
1) composite bacteria agent is added in 30-35 DEG C of the water of 10 times of weight, 200rpm stirs 10min, obtains dilution;
2) rubbish is paved into 40cm thickness, is then sprayed according to every square metre of additive amount using 90g dilution, located The reason time is 72h;
Specifically, the composite bacteria agent is prepared according to following technique:
Aspergillus niger seed liquor and Trichoderma viride seed liquor are mixed according to the volume ratio of 1:3, then according to 10% volume ratio Inoculum concentration, which is gone in fermentor, cultivates for 24 hours, obtains aspergillus niger-Trichoderma viride mixed fermentation liquid;
The Fermenter Medium Component are as follows: molasses 10wt%, cornstarch 3.5wt%, glucose 2.0wt%, biphosphate Sodium 0.2wt%, magnesium sulfate 0.02wt%, manganese sulfate 0.01wt%, surplus are water, pH 7.0;
By Rhodococcus sp, candida utili, coral promise cassette bacterium and Paracoccus denitrificans respectively according to inclined-plane culture, shake Bottle seed culture and fermentation tank culture obtain Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise cassette bacterium hair Zymotic fluid and Paracoccus denitrificans fermentation liquid;
By aspergillus niger-Trichoderma viride mixed fermentation liquid, Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise Cassette fermented liquid and Paracoccus denitrificans fermentation liquid are uniformly mixed according to the volume ratio of 5-7:3-4:3-4:2-3:2-3, are obtained Composite bacteria liquid;
Calcium chloride is added into the boric acid solution that concentration is 1M, and after stirring and dissolving, the pH of solution is adjusted to 7 with sodium carbonate, Obtain crosslinking agent;The boric acid solution and the ratio of calcium chloride are 1L:50g;
It is 30wt% that composite bacteria liquid, which is concentrated into moisture content,;Then the sodium alginate of 3wt% and the poly- second two of 5wt% are added Alcohol, 200rpm stirs 10min, then mixes with crosslinking agent according to the mass ratio of 3:1, is prepared into the microballoon of 1mm, stands 24 at 4 DEG C Hour, then washed 2 times with 0.6wt%NaCl aqueous solution again, 15 DEG C of low temperature dryings to moisture content less than 6wt% to get.
Specifically, the aspergillus niger is ATCC 16404;The Trichoderma viride is ACCC 30166;The Rhodococcus sp is ATCC 15906;The candida utili is ATCC 22023;The coral promise cassette bacterium is ACCC 40100;It is described de- Nitrogen pair coccus is ATCC 13543.
Further, the Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise cassette fermented liquid and The concentration of Paracoccus denitrificans fermentation liquid is controlled 1 × 1010cfu/ml;
Further,
Above-mentioned is preferred embodiment of the invention.As other technologies scheme, the present invention is to bacterial strain in the fermentation liquid of addition Quantity be also not particularly limited, this can need determine according to the environment, for example, when the foul smell in the environment compared with When height is less favorable for the existence of the microbial inoculum, the inoculum concentration of the microbial inoculum can be improved;When the foul smell content in the environment When influence of the lower or described environment to the existence of bacterial strain is smaller, it is possible to reduce inoculum concentration.
For the present invention using Trichoderma viride and aspergillus niger according to 3:1 mixed fermentation, Filter paperlyase can reach 4.06IU/ml, grape Glycosidase is 1.07IU/ml;When using single Trichoderma Viride, Filter paperlyase 2.57IU/ml, glucuroide is 0.34IU/ml;Mixed fermentation is significantly better than single bacterial strain fermentation level, illustrates that two kinds of bacterial strains can play synergistic effect, improves Producing enzyme is horizontal, helps to improve the degradation of agriculture and forestry organic waste material in rubbish.
Strain of the present invention belongs to known bacterial strain, can be commercially available from commercial sources such as ATCC, ACCC.This hair Inclined-plane culture, shake-flask seed culture and the fermentation tank culture of bright each strain are the routine culture mode of this field, are not this hairs Bright innovative point, is not described in detail herein.Raw material used in the present invention or reagent are commercially available in addition to special instruction.
The beneficial effect that the present invention obtains mainly includes but is not limited to the following aspects:
The present invention handles refuse odor using composite bacteria agent, can be effectively removed the gases such as ammonia, hydrogen sulfide, deodorization Effect is good, and operation is simple and feasible;
Composite bacteria agent of the present invention is prepared using carrier and mixed bacteria liquid, and thallus adhesion effect is good, and load capacity is big, compares table Area is big, can improve degradation speed;
The present invention is directed to bacterial strain feature, carries out mixed fermentation to aspergillus niger and Trichoderma viride, compares compared with single bacterial strain fermentation, Improve the vigor of the zymoproteins such as cellulase;
Composite bacteria agent of the invention is to promote the fermentation of house refuse by synergistic effect good between various microorganisms It decomposes, to the foul gas generated such as NH3、H2S carries out absorption degradation, eliminates the evil of house refuse and leachate generation Smelly, the production for fundamentally solving foul gas is raw;
Composite bacteria agent product preparation process of the present invention is simple, and low in cost, good deodorization effect, ecological, environmental protective is easy to use, Production cost is low, without secondary pollution to environment, has broad application prospects.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, having below in conjunction with the application The technical solution of the application is clearly and completely described in body embodiment, it is clear that described embodiment is only this Shen Please a part of the embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not having Every other embodiment obtained under the premise of creative work is made, should fall within the scope of the present invention.
Embodiment 1
A method of refuse odor is administered using composite bacteria agent comprising following steps:
1) composite bacteria agent is added in 30-35 DEG C of the water of 10 times of weight, 200rpm stirs 10min, obtains dilution;
2) rubbish is paved into 30cm thickness, is then sprayed according to every square metre of additive amount using 90g dilution, located The reason time is 72h;
The composite bacteria agent is prepared according to following technique:
By aspergillus niger seed liquor (1 × 108) and Trichoderma viride seed liquor (1 × 10 cfu/ml8Cfu/ml) according to the body of 1:3 Then product goes in fermentor according to the inoculum concentration of 10% volume ratio and cultivates for 24 hours than mixing, obtain aspergillus niger-Trichoderma viride mixing Fermentation liquid;
The Fermenter Medium Component are as follows: molasses 10wt%, cornstarch 3.5wt%, glucose 2.0wt%, biphosphate Sodium 0.2wt%, magnesium sulfate 0.02wt%, manganese sulfate 0.01wt%, surplus are water, pH 7.0;
By Rhodococcus sp, candida utili, coral promise cassette bacterium and Paracoccus denitrificans respectively according to inclined-plane culture, shake Bottle seed culture and fermentation tank culture obtain Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise cassette bacterium hair Zymotic fluid and Paracoccus denitrificans fermentation liquid;
By aspergillus niger-Trichoderma viride mixed fermentation liquid, Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise Cassette fermented liquid and Paracoccus denitrificans fermentation liquid are uniformly mixed according to the volume ratio of 5:3:3:2:2, obtain composite bacteria liquid;Institute State Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise cassette fermented liquid and Paracoccus denitrificans fermentation liquid Concentration is controlled 1 × 1010cfu/ml;
Calcium chloride is added into the boric acid solution that concentration is 1M, and after stirring and dissolving, the pH of solution is adjusted to 7 with sodium carbonate, Obtain crosslinking agent;The boric acid solution and the ratio of calcium chloride are 1L:50g;
It is 30wt% that composite bacteria liquid, which is concentrated into moisture content,;Then the sodium alginate of 3wt% and the poly- second two of 5wt% are added Alcohol, 200rpm stirs 10min, then mixes with crosslinking agent according to the mass ratio of 3:1, is prepared into the microballoon of 1mm, stands 24 at 4 DEG C Hour, then washed 2 times with 0.6wt%NaCl aqueous solution again, 15 DEG C of low temperature dryings to moisture content less than 6wt% to get.
The aspergillus niger is ATCC 16404;The Trichoderma viride is ACCC 30166;The Rhodococcus sp is ATCC 15906;The candida utili is ATCC 22023;The coral promise cassette bacterium is ACCC 40100;The denitrogenation pair Coccus is ATCC 13543.
Embodiment 2
A method of refuse odor is administered using composite bacteria agent comprising following steps:
1) composite bacteria agent is added in 30-35 DEG C of the water of 10 times of weight, 200rpm stirs 10min, obtains dilution;
2) rubbish is paved into 40cm thickness, is then sprayed according to every square metre of additive amount using 90g dilution, located The reason time is 72h;
The composite bacteria agent is prepared according to following technique:
By aspergillus niger seed liquor (1 × 108) and Trichoderma viride seed liquor (1 × 10 cfu/ml8Cfu/ml) according to the body of 1:3 Then product goes in fermentor according to the inoculum concentration of 10% volume ratio and cultivates for 24 hours than mixing, obtain aspergillus niger-Trichoderma viride mixing Fermentation liquid;
The Fermenter Medium Component are as follows: molasses 10wt%, cornstarch 3.5wt%, glucose 2.0wt%, biphosphate Sodium 0.2wt%, magnesium sulfate 0.02wt%, manganese sulfate 0.01wt%, surplus are water, pH 7.0;
By Rhodococcus sp, candida utili, coral promise cassette bacterium and Paracoccus denitrificans respectively according to inclined-plane culture, shake Bottle seed culture and fermentation tank culture obtain Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise cassette bacterium hair Zymotic fluid and Paracoccus denitrificans fermentation liquid;
By aspergillus niger-Trichoderma viride mixed fermentation liquid, Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise Cassette fermented liquid and Paracoccus denitrificans fermentation liquid are uniformly mixed according to the volume ratio of 7:4:4:3:3, obtain composite bacteria liquid;Institute State Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise cassette fermented liquid and Paracoccus denitrificans fermentation liquid Concentration is controlled 1 × 1010cfu/ml;
Calcium chloride is added into the boric acid solution that concentration is 1M, and after stirring and dissolving, the pH of solution is adjusted to 7 with sodium carbonate, Obtain crosslinking agent;The boric acid solution and the ratio of calcium chloride are 1L:50g;
It is 30wt% that composite bacteria liquid, which is concentrated into moisture content,;Then the sodium alginate of 3wt% and the poly- second two of 5wt% are added Alcohol, 200rpm stirs 10min, then mixes with crosslinking agent according to the mass ratio of 3:1, is prepared into the microballoon of 1mm, stands 24 at 4 DEG C Hour, then washed 2 times with 0.6wt%NaCl aqueous solution again, 15 DEG C of low temperature dryings to moisture content less than 6wt% to get.
The aspergillus niger is ATCC 16404;The Trichoderma viride is ACCC 30166;The Rhodococcus sp is ATCC 15906;The candida utili is ATCC 22023;The coral promise cassette bacterium is ACCC 40100;The denitrogenation pair Coccus is ATCC 13543.
Embodiment 3
Composite bacteria agent deodorizing effect test prepared by the embodiment of the present invention 1:
It by the domestic waste of 3000kg corruption, is uniformly mixed, is divided into 3 parts, every part of 1000kg, be respectively placed in big In small 3 the same closed rooms, respectively test group, positive controls and blank control group;Experimental group processing method ginseng See that embodiment 1, positive controls use commercially available EM composite bacteria agent, control group does not add any microbial inoculum.After 72h around rubbish Different acquisition point sampling measures NH3、H2S removal rate, odor concentration.NH3Using sodium hypochlorite-salicylic acid spectrophotometry, H2S Using ammonium alcohol polyvinyl phosphate absorption-methylene-blue colorimetric method, odor concentration is detected using XP-329IIIR fragrance, odor concentration Instrument measurement.Concrete outcome is shown in Table 1:
Table 1
Group NH3 removal rate % H2S removal rate % Odor concentration removal rate %
Test group 96.8 97.6 93.1
Positive controls 92.4 90.3 84.5
Blank control group 8.8 9.3 6.7
Conclusion: composite bacteria agent of the present invention removal ammonia, hydrogen sulfide and foul smell effect are good, are better than existing common product.
Embodiment 4
The compatibility mechanism of each bacterial strain of composite bacteria agent of the present invention:
Experimental group: the processing method of embodiment 1;Control group 1: aspergillus niger-Trichoderma viride mixing is not added in composite bacteria agent Fermentation liquid, remaining is the same as embodiment 1;Control group 2: not adding Rhodococcus sp fermentation liquid in composite bacteria agent, remaining is the same as embodiment 1;Control Group 3: candida utili fermentation liquid is not added in composite bacteria agent, remaining is the same as embodiment 1;Control group 4: it is not added in composite bacteria agent Coral promise cassette fermented liquid, remaining is the same as embodiment 1;Control group 5: not adding Paracoccus denitrificans fermentation liquid in composite bacteria agent, Remaining is the same as embodiment 1.The other deodorizing effect of each group is shown in Table 2:
Table 2
Group NH3 removal rate % H2S removal rate % Odor concentration removal rate %
Experimental group 96.8 97.6 93.1
Control group 1 82.4 70.9 66.8
Control group 2 92.7 83.6 78.1
Control group 3 90.1 82.6 71.5
Control group 4 85.6 92.8 87.4
Control group 5 71.9 82.0 73.5
Conclusion: by upper table 2 as can be seen that each bacterial strain effect of the present invention is good, such as to the foul gas generated NH3、H2S carries out absorption degradation, eliminates the stench of house refuse generation.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (4)

1. a kind of method for administering refuse odor using composite bacteria agent comprising following steps:
1) composite bacteria agent is added in 30-35 DEG C of the water of 10 times of weight, 200rpm stirs 10min, obtains dilution;
2) rubbish is paved into 40cm thickness, is then sprayed according to every square metre of additive amount using 90g dilution, when processing Between be 72h;
The composite bacteria agent is prepared according to following technique:
(1) aspergillus niger seed liquor and Trichoderma viride seed liquor are mixed according to the volume ratio of 1:3, then according to 10% volume ratio Inoculum concentration, which is gone in fermentor, cultivates for 24 hours, obtains aspergillus niger-Trichoderma viride mixed fermentation liquid;
(2) by aspergillus niger-Trichoderma viride mixed fermentation liquid, Rhodococcus sp fermentation liquid, candida utili fermentation liquid, coral promise card Formula fermented liquid and Paracoccus denitrificans fermentation liquid are uniformly mixed according to the volume ratio of 5-7:3-4:3-4:2-3:2-3, are answered Combined bacteria liquid;
(3) calcium chloride is added into the boric acid solution that concentration is 1M, after stirring and dissolving, the pH of solution is adjusted to 7 with sodium carbonate, is obtained To crosslinking agent;The boric acid solution and the ratio of calcium chloride are 1L:50g;
(4) composite bacteria liquid is concentrated into moisture content is 30wt%, then adds the sodium alginate of 3wt% and the poly- second two of 5wt% Alcohol, 200rpm stirs 10min, then mixes with crosslinking agent according to the mass ratio of 3:1, is prepared into the microballoon of 1mm, stands 24 at 4 DEG C Hour, then washed 2 times with 0.6wt%NaCl aqueous solution again, 15 DEG C of low temperature dryings to moisture content less than 6wt% to get;
The aspergillus niger is ATCC 16404;The Trichoderma viride is ACCC 30166;The Rhodococcus sp is ATCC 15906;Institute Stating candida utili is ATCC 22023;The coral promise cassette bacterium is ACCC 40100;The Paracoccus denitrificans is ATCC 13543。
2. the method according to claim 1, wherein the aspergillus niger seed liquor and Trichoderma viride seed liquor is dense Degree is 1 × 108cfu/ml。
3. the method according to claim 1, wherein the component of the fermentation tank culture medium are as follows: molasses 10wt%, Cornstarch 3.5wt%, glucose 2.0wt%, sodium dihydrogen phosphate 0.2wt%, magnesium sulfate 0.02wt%, manganese sulfate 0.01wt%, surplus For water, pH 7.0.
4. the method according to claim 1, wherein the Rhodococcus sp fermentation liquid, candida utili fermentation liquid, The concentration of coral promise cassette fermented liquid and Paracoccus denitrificans fermentation liquid is controlled 1 × 1010cfu/ml。
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CN107236675B (en) * 2017-06-29 2020-05-08 湖北隆创科技有限公司 Microbial preparation for deodorizing municipal domestic garbage
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CN102071140B (en) * 2010-03-26 2012-07-04 无锡中佳科技股份有限公司 Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof
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