CN115286119B - Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof - Google Patents
Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof Download PDFInfo
- Publication number
- CN115286119B CN115286119B CN202210857306.3A CN202210857306A CN115286119B CN 115286119 B CN115286119 B CN 115286119B CN 202210857306 A CN202210857306 A CN 202210857306A CN 115286119 B CN115286119 B CN 115286119B
- Authority
- CN
- China
- Prior art keywords
- solution
- biomass
- hexavalent chromium
- microbial
- clay mineral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers and a preparation method thereof, wherein the microorganism strengthening medicament is prepared from the following raw materials: clay mineral, waste biomass and microbial raw materials. The invention combines the waste biomass material and clay mineral, and adopts the immobilization technology to coat the microbial strain, thereby providing a long-acting nutrient source for the microorganism, increasing the biomass and improving the hexavalent chromium removal capability.
Description
Technical Field
The invention belongs to a microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers and a preparation method thereof, and particularly relates to a microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers and a preparation method thereof.
Background
The traditional treatment of heavy metals by using flocculant, ion exchange method, electrochemical method and biomass adsorbent has the advantages of high cost, narrow application range, different stability and easy secondary pollution. The microorganism strengthening technology is to add nutrient sources such as carbon sources, nitrogen sources and electron donors, so that the microorganism strengthening technology can increase the microorganism biomass and repair heavy metals in water and soil. The exogenous nutrient can assist the growth of microorganisms, but the structure is more unitary, the aging is not long, and the soil is easy to wash by rain water when the soil is repaired and treated. Clay minerals are powerful adsorbents and can act as electron donors for microorganisms and provide nutrition to the microorganisms.
Along with the development of industrialization, the heavy metal content of soil and water source in many chemical plants, industrial aggregation areas and the periphery exceeds the standard, serious injury is caused to plants and animals, and finally the heavy metal enters the human body in a food chain mode, so that various diseases are caused. The microbial remediation method is an environment-friendly technology. The microbial stimulant can strengthen the microorganism by using exogenously added nutrient substrate, surfactant and organic modifier.
Disclosure of Invention
The invention aims to provide a microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as a carrier and a preparation method thereof, which solve the problem of low efficiency of removing hexavalent chromium by microorganisms in the prior art.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a preparation method of a microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers comprises the following steps:
step 1, putting stenotrophomonas in an LB culture medium to obtain a microbial liquid;
step 2, treating the waste biomass raw material by sodium hydroxide, airing, crushing and sieving;
step 3, grinding and sieving the clay mineral raw materials, modifying the clay mineral, drying and finally sieving;
step 4, preparing sodium alginate solution, and mixing the raw materials in the step 2 and the step 3 into the sodium alginate solution in proportion; sterilizing at 120 ℃ for 15min, cooling to room temperature, adding the microbial liquid obtained in the step 1, dripping into 4% calcium chloride solution by using a syringe, crosslinking for 8h, and washing with normal saline to obtain immobilized pellets;
step 5, gelatinizing starch at 80 ℃ to prepare a starch solution, pouring gelatin powder into distilled water to prepare a gelatin solution, and mixing the starch solution and the gelatin solution according to the following weight ratio of 9:1, sterilizing for 15min at 120 ℃, placing the immobilized pellets obtained in the step 4 into the mixed solution, and crosslinking for 12h to obtain the microbial strengthening medicament.
Preferably, the concentration of the sodium hydroxide solution in the step 2 is 0.2% -2%.
Preferably, the clay mineral raw material in the step 3 is kaolin.
Preferably, the raw material ratio of the waste biomass raw material treated by sodium hydroxide and the modified clay mineral in the step 4 is 3:1.
preferably, the clay mineral modification method in the step 3 is as follows: adding chitosan into acetic acid solution, stirring to dissolve completely, adding clay mineral and prepared chitosan acetic acid solution into distilled water, stirring, adjusting pH to 10, adding glutaraldehyde solution, crosslinking for 24 hr, suction filtering, washing with distilled water to pH of 7, and naturally air drying.
Preferably, the usage ratio of the clay mineral to distilled water is 1g:10ML.
The invention adopts another technical scheme that: a microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers is prepared from the following raw materials: modified clay mineral, waste biomass raw material treated by sodium hydroxide, stenotrophomonas, sodium alginate, calcium chloride, starch solution and gelatin solution.
The invention has the beneficial effects that: the invention combines the waste biomass material and clay mineral, adopts the immobilization technology to coat the microbial strain, provides a long-acting nutrient source for the microorganism, increases the biomass and improves the hexavalent chromium removal capacity; the invention has the characteristics of long-acting, low cost, environment friendliness, waste recycling and pollution control by waste.
Drawings
FIG. 1 is a schematic flow chart of a method for preparing a microorganism-enhanced chemical for removing hexavalent chromium by using minerals/biomass as carriers.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
A microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers is prepared from the following raw materials: modified clay mineral, waste biomass raw material treated by sodium hydroxide, stenotrophomonas, sodium alginate, calcium chloride, starch solution and gelatin solution.
As shown in fig. 1, a preparation method of a microorganism strengthening agent for removing hexavalent chromium by using minerals/biomass as a carrier comprises the following steps:
and step 1, placing the microbial strain in an LB culture medium for independent culture.
And 2, taking waste biomass as a raw material. Treating the straws, peanut shells and pericarps with 0.2% -2% sodium hydroxide, sun-drying, crushing, sieving, and mixing the straws, the peanut shells and the pericarps according to the proportion of 3:3: 1. and uniformly mixing to obtain the mixed biomass material.
And step 3, taking kaolin as a clay mineral raw material, grinding and sieving, and modifying the clay mineral. The method comprises the following specific steps: to 50ml of 2% acetic acid solution, 1g of chitosan was added, and the mixture was stirred with a magnetic stirrer until complete dissolution. Adding 10g of clay mineral and prepared chitosan solution into 100ml of distilled water, stirring for 30min, adjusting pH to mixed solution Ph to 10 with 0.1mol/L sodium hydroxide solution, adding glutaraldehyde solution, crosslinking for 24 hours, filtering, washing with distilled water until pH is 7, drying, and sieving.
Step 4, preparing 4% sodium alginate solution, and mixing the raw materials in the step 2 and the step 3 according to the following weight ratio of 1: 1 (mixing biomass materials: modified clay minerals) in proportion into sodium alginate solution, and uniformly mixing by an ultrasonic cleaner; sterilizing at 120 ℃ for 15min, cooling to room temperature, adding 1ml of the microbial liquid in the step 1, dripping into 4% calcium chloride solution by using a syringe, crosslinking for 8h, and washing with 0.7% -1% physiological saline to obtain the immobilized pellet.
Step 5, gelatinizing starch at 80 ℃ to prepare a 2% starch solution, pouring gelatin powder into distilled water to prepare a 1% gelatin solution, and mixing the starch solution and the gelatin solution according to 9:1, adding 2% glycerol solution, sterilizing at 120 ℃ for 15min, placing the immobilized beads obtained in the step 4 into the mixed solution, and crosslinking for 12h to obtain the microbial strengthening medicament.
Example 2
A microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers is prepared from the following raw materials: modified clay mineral, waste biomass raw material treated by sodium hydroxide, stenotrophomonas, sodium alginate, calcium chloride, starch solution and gelatin solution.
As shown in fig. 1, a preparation method of a microorganism strengthening agent for removing hexavalent chromium by using minerals/biomass as a carrier comprises the following steps:
and step 1, placing the microbial strain in an LB culture medium for independent culture.
And 2, taking waste biomass as a raw material. Treating the straws, peanut shells and pericarps with 0.2% -2% sodium hydroxide, sun-drying, crushing, sieving, and mixing the straws, the peanut shells and the pericarps according to the proportion of 3:3: 1. and uniformly mixing to obtain the mixed biomass material.
And step 3, taking kaolin as a clay mineral raw material, grinding and sieving, and modifying the clay mineral. The method comprises the following specific steps: to 50ml of 2% acetic acid solution, 1g of chitosan was added, and the mixture was stirred with a magnetic stirrer until complete dissolution. Adding 10g of clay mineral and prepared chitosan solution into 100ml of distilled water, stirring for 30min, adjusting pH to mixed solution Ph to 10 with 0.1mol/L sodium hydroxide solution, adding glutaraldehyde solution, crosslinking for 24 hours, filtering, washing with distilled water until pH is 7, drying, and sieving.
Step 4, preparing 4% sodium alginate solution, and mixing the raw materials in the step 2 and the step 3 according to the following weight ratio of 2: 1 (mixing biomass materials: modified clay minerals) in proportion into sodium alginate solution, and uniformly mixing by an ultrasonic cleaner; sterilizing at 120 ℃ for 15min, cooling to room temperature, adding 1ml of the microbial liquid in the step 1, dripping into 4% calcium chloride solution by using a syringe, crosslinking for 8h, and washing with 0.7% -1% physiological saline to obtain the immobilized pellet.
Step 5, gelatinizing starch at 80 ℃ to prepare a 2% starch solution, pouring gelatin powder into distilled water to prepare a 1% gelatin solution, and mixing the two solutions according to 9:1, adding 2% glycerol solution, sterilizing at 120 ℃ for 15min, placing the immobilized beads obtained in the step 4 into the mixed solution, and crosslinking for 12h to obtain the microbial strengthening medicament.
Example 3
A microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers is prepared from the following raw materials: modified clay mineral, waste biomass raw material treated by sodium hydroxide, stenotrophomonas, sodium alginate, calcium chloride, starch solution and gelatin solution.
As shown in fig. 1, a preparation method of a microorganism strengthening agent for removing hexavalent chromium by using minerals/biomass as a carrier comprises the following steps:
and step 1, placing the microbial strain in an LB culture medium for independent culture.
And 2, taking waste biomass as a raw material. Treating the straws, peanut shells and pericarps with 0.2% -2% sodium hydroxide, sun-drying, crushing, sieving, and mixing the straws, the peanut shells and the pericarps according to the proportion of 3:3: 1. and uniformly mixing to obtain the mixed biomass material.
And step 3, taking kaolin as a clay mineral raw material, grinding and sieving, and modifying the clay mineral. The method comprises the following specific steps: to 50ml of 2% acetic acid solution, 1g of chitosan was added, and the mixture was stirred with a magnetic stirrer until complete dissolution. Adding 10g of clay mineral and prepared chitosan solution into 100ml of distilled water, stirring for 30min, adjusting pH to mixed solution Ph to 10 with 0.1mol/L sodium hydroxide solution, adding glutaraldehyde solution, crosslinking for 24 hours, filtering, washing with distilled water until pH is 7, drying, and sieving.
Step 4, preparing 4% sodium alginate solution, and mixing the raw materials in the step 2 and the step 3 according to the following weight ratio of 3:1 (mixing biomass materials: modified clay minerals) in proportion into sodium alginate solution, and uniformly mixing by an ultrasonic cleaner; sterilizing at 120 ℃ for 15min, cooling to room temperature, adding 1ml of the microbial liquid in the step 1, dripping into 4% calcium chloride solution by using a syringe, crosslinking for 8h, and washing with 0.7% -1% physiological saline to obtain the immobilized pellet.
Step 5, gelatinizing starch at 80 ℃ to prepare a 2% starch solution, pouring gelatin powder into distilled water to prepare a 1% gelatin solution, and mixing the two solutions according to 9:1, adding 2% glycerol solution, sterilizing at 120 ℃ for 15min, placing the immobilized beads obtained in the step 4 into the mixed solution, and crosslinking for 12h to obtain the microbial strengthening medicament.
Experimental example 1
The microbial strengthening agent prepared in example 1 has influence on hexavalent chromium pollution in water, and has the following experimental steps:
step 1, preparing 50g/L hexavalent chromium mother liquor by potassium dichromate for later use.
And 2, pouring the mother solution into 1L of the sterilized LB culture solution, and preparing the initial hexavalent chromium concentration to be 100 mg/L or 300mg/L. The hexavalent chromium removal rate was measured by placing the microbial enhancement agent of example 1 into a 100mL conical flask of hexavalent chromium having an initial concentration of 100 and 300mg/L, using Stenotrophomonas as a pure bacterial control, and using a blank agent without bacteria (the same composition as the microbial enhancement agent of example 1 except for the absence of Stenotrophomonas) as a parallel control, as shown in table 1.
TABLE 1
As can be seen from Table 1, the microorganism enhancing agent has a higher hexavalent chromium removal rate than Stenotrophomonas (Stenotrophomonas) using the method of the present invention; when the initial concentration of hexavalent chromium is 100 mg/L and 300mg/L, the removal rate of the microorganism strengthening agent on hexavalent chromium is 88% and 59.7% respectively, and the removal rate is increased by 4.3% and 3.4% respectively compared with that of Stenotrophomonas (Stenotrophomonas).
Experimental example 2
The microbial strengthening agent prepared in example 2 has influence on hexavalent chromium pollution in water, and has the following experimental steps:
step 1, preparing 50g/L hexavalent chromium mother liquor by potassium dichromate for later use.
And 2, pouring the mother solution into 1L of the sterilized LB culture solution, and preparing the initial hexavalent chromium concentration to be 100 mg/L or 300mg/L. The hexavalent chromium removal rate was measured by placing the microbial enhancement agent of example 2 (same as the production method of example 1) in a 100mL conical flask of hexavalent chromium having an initial concentration of 100 and 300mg/L, using Stenotrophomonas as a pure bacterial control, and using a blank agent without bacteria (the same components as the microbial enhancement agent of example 1 except for the absence of Stenotrophomonas) as a parallel control, as shown in table 2.
TABLE 2
As can be seen from Table 2, the microorganism enhancing agent has a higher hexavalent chromium removal rate than Stenotrophomonas (Stenotrophomonas) using the method of the present invention; when the initial concentration of hexavalent chromium is 100 mg/L and 300mg/L, the removal rate of the microorganism strengthening agent on hexavalent chromium is 95.6 percent and 65.2 percent respectively, and the removal rate is increased by 11.9 percent and 8.9 percent respectively compared with that of Stenotrophomonas (Stenotrophomonas).
Experimental example 3
The microbial strengthening agent prepared in example 3 has influence on hexavalent chromium pollution in water, and has the following experimental steps:
step 1, preparing 50g/L hexavalent chromium mother liquor by potassium dichromate for later use.
And 2, pouring the mother solution into 1L of the sterilized LB culture solution, and preparing the initial hexavalent chromium concentration to be 100 mg/L or 300mg/L. The hexavalent chromium removal rate was measured by placing the microbial enhancement agent of example 3 (same as the production method of example 1) in a 100mL conical flask of hexavalent chromium having an initial concentration of 100 and 300mg/L, using Stenotrophomonas as a pure bacterial control, and using a blank agent without bacteria (the same components as the microbial enhancement agent of example 1 except for the absence of Stenotrophomonas) as a parallel control, as shown in table 3.
TABLE 3 Table 3
As can be seen from Table 3, the microorganism enhancing agent has a higher hexavalent chromium removal rate than Stenotrophomonas (Stenotrophomonas) using the method of the present invention; when the initial concentration of hexavalent chromium is 100 mg/L and 300mg/L, the removal rate of the microorganism strengthening agent on hexavalent chromium is 98% and 70.3% respectively, and is increased by 14.3% and 11.1% respectively compared with the removal rate of Stenotrophomonas (Stenotrophomonas).
From this, it can be seen from tables 1 to 3 that the ingredient ratio of example 3 is optimal.
The invention combines the waste biomass material and clay mineral, and adopts the immobilization technology to coat the microbial strain, thereby providing a long-acting nutrient source for the microorganism, increasing the biomass and improving the hexavalent chromium removal capability. The invention has the characteristics of long-acting, low cost, environment protection, waste recycling and pollution control by waste.
While the present invention has been described in detail through the foregoing description of the preferred embodiment, it should be understood that the foregoing description is not to be considered as limiting the invention. Many modifications and substitutions of the present invention will become apparent to those of ordinary skill in the art upon reading the foregoing. Accordingly, the scope of the invention should be limited only by the attached claims.
Claims (7)
1. The preparation method of the microbial strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carriers is characterized by comprising the following steps of:
step 1, putting stenotrophomonas in an LB culture medium to obtain a microbial liquid;
step 2, treating the waste biomass raw material by sodium hydroxide, airing, crushing and sieving;
step 3, grinding and sieving the clay mineral raw materials, modifying the clay mineral, drying and finally sieving;
step 4, preparing sodium alginate solution, and mixing the raw materials in the step 2 and the step 3 into the sodium alginate solution in proportion; sterilizing at 120 ℃ for 15min, cooling to room temperature, adding the microbial liquid obtained in the step 1, dripping into 4% calcium chloride solution by using a syringe, crosslinking for 8h, and washing with normal saline to obtain immobilized pellets;
step 5, gelatinizing starch at 80 ℃ to prepare a starch solution, pouring gelatin powder into distilled water to prepare a gelatin solution, and mixing the starch solution and the gelatin solution according to the following weight ratio of 9:1, sterilizing for 15min at 120 ℃, placing the immobilized pellets obtained in the step 4 into the mixed solution, and crosslinking for 12h to obtain the microbial strengthening medicament.
2. The method for preparing a microbial strengthening agent for hexavalent chromium removal using a mineral/biomass as a carrier according to claim 1, wherein the concentration of the sodium hydroxide solution in the step 2 is 0.2% to 2%.
3. The method for preparing a microbial strengthening agent for removing hexavalent chromium using a mineral/biomass as a carrier according to claim 2, wherein the clay mineral raw material in the step 3 is kaolin.
4. The method for preparing a microbial strengthening agent for hexavalent chromium removal using minerals/biomasses as a carrier according to claim 1, wherein the raw material ratio of the waste biomass raw material treated with sodium hydroxide and the modified clay mineral in the step 4 is 3:1.
5. the method for preparing the microbial strengthening agent for removing hexavalent chromium by using minerals/biomass as carriers according to claim 1, wherein the method for modifying clay minerals in the step 3 is as follows: adding chitosan into acetic acid solution, stirring to dissolve completely, adding clay mineral and prepared chitosan acetic acid solution into distilled water, stirring, adjusting pH to 10, adding glutaraldehyde solution, crosslinking for 24 hr, suction filtering, washing with distilled water to pH of 7, and naturally air drying.
6. The method for preparing a microbial strengthening agent for hexavalent chromium removal using a mineral/biomass as a carrier according to claim 5, wherein the ratio of the clay mineral to distilled water is 1g:10mL.
7. A microbial strengthening agent prepared by the method for preparing a microbial strengthening agent for hexavalent chromium removal using minerals/biomass as a carrier according to any one of claims 1 to 6, wherein the microbial strengthening agent is prepared from the following raw materials: modified clay mineral, waste biomass raw material treated by sodium hydroxide, stenotrophomonas, sodium alginate, calcium chloride, starch solution and gelatin solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210857306.3A CN115286119B (en) | 2022-07-20 | 2022-07-20 | Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210857306.3A CN115286119B (en) | 2022-07-20 | 2022-07-20 | Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115286119A CN115286119A (en) | 2022-11-04 |
| CN115286119B true CN115286119B (en) | 2023-09-12 |
Family
ID=83824755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210857306.3A Active CN115286119B (en) | 2022-07-20 | 2022-07-20 | Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115286119B (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045581A (en) * | 2012-12-21 | 2013-04-17 | 淮阴工学院 | Method for immobilizing myxococcus fulvus coated by chitosan loaded with attapulgite |
| CN104386831A (en) * | 2014-12-03 | 2015-03-04 | 西南科技大学 | Method of treating hexavalent chromium wastewater by immobilized microorganisms |
| CN104431370A (en) * | 2014-12-15 | 2015-03-25 | 南京优帆生物科技有限公司 | Efficient probiotics microcapsule as well as preparation method and application thereof |
| CN107828771A (en) * | 2017-11-21 | 2018-03-23 | 山东科技大学 | A kind of preparation method of immobilization oil degradation bacteria and the method based on immobilization oil degradation bacteria processing petroleum pollution |
| CN108018229A (en) * | 2017-11-09 | 2018-05-11 | 中南大学 | A kind of hexavalent chrome reduction immobilized microbial inoculum and preparation method |
| CN108018280A (en) * | 2017-12-04 | 2018-05-11 | 博天环境集团股份有限公司 | A kind of construction method of microorganism slow-released carrier |
| CN108558026A (en) * | 2018-04-20 | 2018-09-21 | 裴泽民 | A kind of preparation method of algicide |
| CN110172456A (en) * | 2019-05-28 | 2019-08-27 | 中南大学 | It is a kind of with molten algae, degrade algae toxin and the biomaterial for denitrogenating phosphorus effect and its preparation method and application |
| CN110241111A (en) * | 2019-06-17 | 2019-09-17 | 中南大学 | A kind of method of modified activated carbon immobilized cell enhancing bacterium chromium reducing power |
| CN110791498A (en) * | 2019-08-20 | 2020-02-14 | 中国环境科学研究院 | Immobilization method of stenotrophomonas and composition prepared by same |
| CN110938569A (en) * | 2019-12-11 | 2020-03-31 | 山东科技大学 | Microbial agent for hexavalent chromium pollution treatment and hexavalent chromium pollution treatment method |
| CN111117997A (en) * | 2020-01-06 | 2020-05-08 | 朱扣 | Method for immobilizing microorganisms by carrier |
| FR3097231A1 (en) * | 2019-06-11 | 2020-12-18 | Orano Mining | New strain of bacteria of the genus Stenotrophomonas, material comprising these bacteria and their uses for the decontamination of uranium (VI) in water and aqueous effluents |
| CN113430190A (en) * | 2021-05-06 | 2021-09-24 | 玖胜丰(潍坊)生物技术开发有限公司 | Preparation method and application of composite microsphere containing paenibacillus polymyxa sodium alginate |
| WO2022131847A1 (en) * | 2020-12-18 | 2022-06-23 | 일동제약(주) | Non-stop production process for improving freeze-drying survival, heat tolerance, shelf stability and digestive stability of probiotics using spontaneous matrix-encapsulation technique |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9994470B2 (en) * | 2011-03-07 | 2018-06-12 | General Electric Company | Method of removing recalcitrant organic pollutants |
-
2022
- 2022-07-20 CN CN202210857306.3A patent/CN115286119B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045581A (en) * | 2012-12-21 | 2013-04-17 | 淮阴工学院 | Method for immobilizing myxococcus fulvus coated by chitosan loaded with attapulgite |
| CN104386831A (en) * | 2014-12-03 | 2015-03-04 | 西南科技大学 | Method of treating hexavalent chromium wastewater by immobilized microorganisms |
| CN104431370A (en) * | 2014-12-15 | 2015-03-25 | 南京优帆生物科技有限公司 | Efficient probiotics microcapsule as well as preparation method and application thereof |
| CN108018229A (en) * | 2017-11-09 | 2018-05-11 | 中南大学 | A kind of hexavalent chrome reduction immobilized microbial inoculum and preparation method |
| CN107828771A (en) * | 2017-11-21 | 2018-03-23 | 山东科技大学 | A kind of preparation method of immobilization oil degradation bacteria and the method based on immobilization oil degradation bacteria processing petroleum pollution |
| CN108018280A (en) * | 2017-12-04 | 2018-05-11 | 博天环境集团股份有限公司 | A kind of construction method of microorganism slow-released carrier |
| CN108558026A (en) * | 2018-04-20 | 2018-09-21 | 裴泽民 | A kind of preparation method of algicide |
| CN110172456A (en) * | 2019-05-28 | 2019-08-27 | 中南大学 | It is a kind of with molten algae, degrade algae toxin and the biomaterial for denitrogenating phosphorus effect and its preparation method and application |
| FR3097231A1 (en) * | 2019-06-11 | 2020-12-18 | Orano Mining | New strain of bacteria of the genus Stenotrophomonas, material comprising these bacteria and their uses for the decontamination of uranium (VI) in water and aqueous effluents |
| CN110241111A (en) * | 2019-06-17 | 2019-09-17 | 中南大学 | A kind of method of modified activated carbon immobilized cell enhancing bacterium chromium reducing power |
| CN110791498A (en) * | 2019-08-20 | 2020-02-14 | 中国环境科学研究院 | Immobilization method of stenotrophomonas and composition prepared by same |
| CN110938569A (en) * | 2019-12-11 | 2020-03-31 | 山东科技大学 | Microbial agent for hexavalent chromium pollution treatment and hexavalent chromium pollution treatment method |
| CN111117997A (en) * | 2020-01-06 | 2020-05-08 | 朱扣 | Method for immobilizing microorganisms by carrier |
| WO2022131847A1 (en) * | 2020-12-18 | 2022-06-23 | 일동제약(주) | Non-stop production process for improving freeze-drying survival, heat tolerance, shelf stability and digestive stability of probiotics using spontaneous matrix-encapsulation technique |
| CN113430190A (en) * | 2021-05-06 | 2021-09-24 | 玖胜丰(潍坊)生物技术开发有限公司 | Preparation method and application of composite microsphere containing paenibacillus polymyxa sodium alginate |
Non-Patent Citations (1)
| Title |
|---|
| 环链棒束孢(Isaria cateniannulata)杀虫缓释剂的制作;张杰;赵丹晨;张丽红;李丰伯;;江苏农业科学;48(03);第119-124页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115286119A (en) | 2022-11-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| El-Sheekh et al. | Biosorption of cadmium from aqueous solution by free and immobilized dry biomass of Chlorella vulgaris | |
| CN108359663B (en) | Phosphorus-accumulating bacterium immobilized pellet and application thereof | |
| CN110592066B (en) | Charcoal-loaded nano zero-valent iron coupled phosphate solubilizing bacterium immobilized pellet and preparation method and application thereof | |
| CN105753171A (en) | Method for treating heavy metal wastewater | |
| CN107555607B (en) | Biological composite oxygen increasing agent and preparation method thereof | |
| CN108101228A (en) | A kind of wastewater treatment microbial microbial inoculum | |
| CN108192889B (en) | Method for treating wastewater by using bacterial cellulose immobilized microalgae | |
| CN107937382B (en) | Preparation method of immobilized microalgae | |
| CN113562846B (en) | Denitrification efficient carbon source and processing technology thereof | |
| CN104894033A (en) | Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant | |
| CN115385456B (en) | Efficient denitrification sewage treatment agent and preparation method thereof | |
| CN107673483B (en) | Biological treatment method for copper-polluted seawater | |
| Chen et al. | Enhanced treatment of organic matters in starch wastewater through Bacillus subtilis strain with polyethylene glycol-modified polyvinyl alcohol/sodium alginate hydrogel microspheres | |
| Li et al. | Research progress on the application of natural adsorbents in the treatment of livestock wastewater | |
| CN115286119B (en) | Microorganism strengthening medicament for removing hexavalent chromium by taking minerals/biomass as carrier and preparation method thereof | |
| CN109052650B (en) | Preparation of immobilized microalgae water quality control agent | |
| CN116371183A (en) | Method for removing odor generated by anaerobic fermentation of compost through biological filter | |
| CN114134043A (en) | Preparation method of environment-friendly sewage treatment agent | |
| CN108191078A (en) | A kind of slow-release nutrient micelle for being exclusively used in microorganism treating sewage and preparation method | |
| CN114105310A (en) | Preparation method and application of biological microcapsule | |
| CN110357266B (en) | Method for removing heavy metal ions in polluted water body by using sargassum thunbergii | |
| Liu et al. | Mechanism regulation, production and potential of high value substances in the wastewater treatment by immobilized photosynthetic bacteria: A review | |
| CN112375721A (en) | Preparation method and application of composite microbial inoculum for low-temperature treatment of rural domestic sewage | |
| CN112028251A (en) | Method for improving ammonia nitrogen removal effect of microalgae on livestock and poultry breeding wastewater | |
| CN112126439A (en) | Immobilized microorganism soil remediation agent and soil remediation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |