CN101429487A - M-sporangiocyst bacterium Q5-1 for purifying chromium pollution in wastewater and uses thereof - Google Patents
M-sporangiocyst bacterium Q5-1 for purifying chromium pollution in wastewater and uses thereof Download PDFInfo
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- CN101429487A CN101429487A CNA2008102366799A CN200810236679A CN101429487A CN 101429487 A CN101429487 A CN 101429487A CN A2008102366799 A CNA2008102366799 A CN A2008102366799A CN 200810236679 A CN200810236679 A CN 200810236679A CN 101429487 A CN101429487 A CN 101429487A
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Abstract
The invention belongs to the technical field of environmental microorganisms, and particularly relates to Intrasporangiaceae sp Q5-1 with reduction effect on hexavalent chromium and application of the Intrasporangiaceae sp Q5-1 in the purification of chromium pollution in wastewater. The invention is characterized in that the Intrasporangiaceae sp Q5-1 which has reduction effect on the hexavalent chromium is obtained through the separation from manganese mine soil in Hunan province. The strain can reduce the hexavalent chromium in wastewater containing chromium into trivalent chromium, which greatly reduces the toxicity of the chromium in the wastewater. The strain is named the Intrasporangiaceae sp Q5-1, and is a newly discovered chromium-reducing bacterium, and CCTCC NO of the strain is M208239. The preliminary study shows that the strain has better application prospect in the treatment of the chromium pollution in the wastewater.
Description
Technical field
The invention belongs to the environmental microorganism technical field, be specifically related to a strain to inorganic sexavalent chrome have reductive action between sporangiocyst bacterium Q5-1 screening and aspect the purifying chromium-containing contaminated wastewater in purposes.
Background technology
Heavy metal contamination has at present become a global problem, and in heavy metal contamination, chromium is as the extremely strong a kind of pollutent of heavy metal poisoning, and its processing and recovery become the emphasis that the countries in the world scientist studies heavy metal contamination especially.Chromium is one of industrial metal commonly used, be mainly used in aspects such as plating and special smelting, in addition, chromic salts also can be used as industrial saddle agent, pigment, catalyzer, go out mould dose, wood preservative or the like, continuous development along with world industry, increasing chromium (VI) is discharged in the physical environment, has caused the serious environmental pollution.
Usually having only two kinds of stable valence states to occur in the physical environment is trivalent and sexavalence.Chromic toxicity is higher approximately 100 times than trivalent chromium, can be reduced to trivalent chromium, is that trivalent chromium is a toxicide process with hexavalent chrome reduction.At pollution of chromium, the processing of traditional chromate waste water mainly contains two kinds: a kind of is to change chromium (VI) to have a form in water, making deliquescent metallic transition is the metallic compound of insoluble or insoluble, removes from waste water, as chemical reduction method, electrochemical process etc.; The 2nd, do not change the form that exists of chromium (VI), (VI) removes from waste water with chromium, as ion exchange method, active carbon adsorption or the like.These methods are in that to exist investment in varying degrees big, and the working cost height is administered back shipwreck shortcoming up to standard or the like, and produced a large amount of mud, causes secondary pollution.Micro-reduction method dechromisation (VI) has bigger application potential as a kind of more emerging method for removing chromium.The micro-reduction method be meant utilize be in the efficient function yeast of growth conditions to the enzyme catalysis reduction of chromium (VI), bacterial metabolism product to the effects such as electrostatic adhesion, complexing, flocculation and precipitation of the reduction of Cr (VI) and thalline to chromium (VI), make Cr (VI) transform (III) for precipitating, through solid-liquid separation, realize purification of waste water.This method processing waste water containing chrome is finished in microbial growth Metabolic activity process.The more bacterium of report is under anaerobic to have chromium (VI) reduction effect preferably at present, but anaerobic condition is more harsh, be unfavorable for practical application, of the present invention sporangiocyst bacterium Q5-1 can be at the trivalent chromium a little less than under the aerobic condition hexavalent chrome reduction of strong toxicity being toxicity, greatly reduce the toxicity of chromium in the environment, for the effect that improves the microbial treatment pollution of chromium and the development of pollution of chromium microbial technique significance is arranged, the application that in the processing of high concentration chrome wastewater etc., also has.
Summary of the invention
The objective of the invention is to overcome the defective of pollution of chromium purification techniques in the existing waste water, separate and obtain the novel chromium reduction of strain bacterium, this bacterial strain can be the trivalent chromium of low toxicity with the hexavalent chrome reduction of high poison in the waste water, and separates out with precipitation or complex form, thus the pollution of chromium in purifying liquid waste.The invention still further relates to its purposes.
The present invention is achieved through the following technical solutions:
The applicant separates, screens the novel chromium reduction of strain bacterium, this bacterial strain is named as Q5-1, sporangiocyst bacterium (Intrasporangiaceae) between genus, sporangiocyst bacterium between this bacterial strain (Intrasporangiaceae sp.) Q5-1 delivers Chinese typical culture collection center (CCTCC) preservation in the Wuhan University of Wuhan City, Hubei Province on November 25th, 2008, and its preserving number is: CCTCC NO:M208239.
Between the screening scheme of sporangiocyst bacterium Q5-1 referring to accompanying drawing 1.By accompanying drawing 1, take Hunan China to economize a manganese ore pedotheque earlier, add finite concentration (detailed description of seeing below, down together) K
2CrO
4Carry out enrichment culture, again the soil sample of enrichment culture is diluted and be coated with and contain finite concentration K
2CrO
4General LB solid medium flat board; cultivation grows chromium resistance bacterium; the bacterium colony of picking different shape rule mono-clonal; use the chromic mensuration-diphenyl carbazide spectrophotometry of water quality (State Bueau of Environmental Protection again; State Standard of the People's Republic of China GB7467-87; Beijing; China Standard Press; November in 1987 the 1st edition) detect and hexavalent chrome reduction can be reduced to chromic bacterial strain; again detected chromium reduction bacterium is cooked 16S ribosomal RNA gene (16S rDNA); morphology and relevant evaluation work such as functional gene finally obtain a sporangiocyst bacterium Q5-1.
More detailed technological step is as follows:
(1) sample is taked: in early July, 2007 is taked Hunan Province's one manganese ore pedotheque.
(2) example enrichment: accurately take by weighing soil sample 100g in 250ml sterilization triangular flask, add the K of 5ml 100mM
2CrO
4, stir evenly gently to put in 37 ℃ of incubators and cultivate a week, note adding sterilized water, guarantee that sample is dried.
(3) chromium resistance bacterium separates: accurately take by weighing K
2CrO
4Enrichment soil sample 10g puts in 37 ℃ of shaking tables and vibrates half an hour in the triangular flask that the 90ml stroke-physiological saline solution is housed, and gets 1ml more successively and progressively be diluted to 10 in the 9ml stroke-physiological saline solution
-4, 10
-5, 10
-6, get the K that the 0.1ml coating contains 1mM respectively
2CrO
4LB solid medium flat board, 3 flat boards of each extent of dilution coating are put in 37 ℃ of incubators and are cultivated a week, the bacterial strain that grows is a chromium resistance bacterium, puts in 4 ℃ of refrigerators flat board stand-by.LB solid culture based formulas is as follows, in the 1L solution, and Tryptones (Tryptone): 10g; Yeast extract (ctYeast Extra): 5g; Sodium-chlor (NaCl): l0g; Agar, 15g.
(4) line separates: the different bacterium colony of chromium resistance bacterium picking that obtains in the step (3) is rule on the LB solid plate, guarantee to obtain mono-clonal, treat to put after bacterium grows in 4 ℃ of refrigerators stand-by and a in-80 ℃ of refrigerators with the preservation of glycerine freeze pipe.
(5) chromium reduction bacterium screening: the chromium resistance bacterium mono-clonal that obtains in the step (4) is transferred in the 10ml LB liquid nutrient medium, adds the K of 100ul100mM
2CrO
4, making its final concentration is 1mM, puts shaking culture in 37 ℃ of shaking tables, and timing.Beginning for some time is somatic cells vegetative period, does not almost have iuvenescence, and it enters the logarithmic growth after date by the time, and cell density increases, and rate of reduction begins to accelerate, and detects from this moment beginning cluster sampling.Sampling procedure is as follows: under aseptic condition, get 1.5ml bacterium liquid in the 2ml centrifuge tube, 12000rpm got supernatant in centrifugal 4 minutes and detects.Got one time sample every two hours.The measuring method of chromium in the supernatant (VI) all adopts the chromic mensuration-diphenyl carbazide spectrophotometry of water quality (State Bueau of Environmental Protection; State Standard of the People's Republic of China GB7467-87; Beijing; China Standard Press; November in 1987 the 1st edition); specific as follows: as to get supernatant 1ml and place the 50ml colorimetric cylinder, be diluted with water to graticule.Add 0.5ml sulphuric acid soln (1:1) and 0.5ml phosphoric acid solution (1:1), shake up.Add 2ml diphenylcarbazide developer, shake up, after 5～10 minutes, in 540nm wavelength place, water is cooked reference, measures absorbancy, checks in corresponding content of 6-valence Cr ions from typical curve.It is the content of 6-valence Cr ions that has been reduced that initial value deducts residual value shi.Under the same terms, the reductive sexavalent chrome is many more, and the reductibility of bacterium is strong more.
(6) classification of chromium reduction bacterium is identified: the one, and utilize 16S rDNA to identify, promptly adopt prokaryotic organism 16S rDNA universal primer 27F (being forward primer): 5 ' AGAGTTTGATCMTGGCTCAG3 ') and 1492R (reverse primer): 5 ' GGYTACCTTGTTACGACTT3 ' be PCR (concrete operation method of PCR be referring to applicant's granted patent document, and the patent No. is the ZL200510120584.7 denomination of invention: a kind of small quality fast extraction method for soil total DNA).Increase its 16S rDNA and order-checking, with international NCBI GenBank (www.ncbi.nlm.nih.gov) Nucleotide database comparison, nucleotide homology is 97%, is accredited as a sporangiocyst bacterium again; The 2nd, gramstaining analysis and growth characteristics are identified.
Sporangiocyst bacterium (Intrasporangiaceae sp.) Q5-1 mycology feature is as follows between the present invention's preparation:
Between sporangiocyst bacterium (Intrasporangiaceae sp.) Q5-1 be Gram-negative bacteria (the gramstaining photo is seen shown in the accompanying drawing 2), 37 ℃ of suitable growth temperatures, appropriate pH 8.0 is on the LB solid medium that little Huang is transparent, circular, the bacterium colony of projection.
Between the preservation of sporangiocyst bacterium Q5-1:
Between sporangiocyst bacterium Q5-1 can under 4 ℃, make short term storage after the cultivation 37 ℃ of cultivations on general LB liquid or solid substratum.If long-term preservation, can use glycerine freeze pipe or lyophilize pipe (referring to Zhao Bin, He Shaojiang. the microbiology experiment. first version. the .2002:202 of Science Press-205) preservation strain is proper.
Positively effect of the present invention is:
Sexavalent chrome is compared with trivalent chromium, and the former toxicity is higher more than 100 times than the latter, and sexavalent chrome generally exists with ionic species and be difficult for to remove, and trivalent chromium generally exists with precipitation or complex form, more easily removes.Separation screening of the present invention between sporangiocyst bacterium Q5-1 can be under aerobic condition be trivalent chromium with hexavalent chrome reduction, greatly reduced the harm of chromium in the waste water, can also be expected in purifying liquid waste, play a significant role aspect the pollution of chromium in conjunction with pollution of chromium in the immobilization embedded technology governance waste water of bacterium.
Description of drawings
Fig. 1: be technological line figure of the present invention.
Fig. 2: be of the present invention sporangiocyst bacterium Q5-1 gramstaining photo.
Fig. 3: be the chromium reduction graphic representation of of the present invention sporangiocyst bacterium Q5-1, among the figure: X-axis represent the time (hour), hexavalent chromium concentration (mg/1) is represented on the Y-axis left side, represents cell density (OD on the right of the Y-axis
600).
Fig. 4: be of the present invention sporangiocyst bacterium Q5-1 in the simulation chromium pollution water to the chromic reduction graphic representation that adds up, among the figure: X-axis represent the time (hour), the Y-axis representative remains content of 6-valence Cr ions (%).
Fig. 5: be that of the present invention sporangiocyst bacterium Q5-1 carries out immobilization embedded back to chromic reduction graphic representation, among the figure: X-axis represent the time (hour), the Y-axis representative remains content of 6-valence Cr ions (%).
Embodiment
The isolation identification of embodiment 1 chromium reduction bacterium
(1) sample is taked: in early July, 2007 takes Hunan China to economize the pedotheque of a manganese ore.
(2) example enrichment: accurately take by weighing soil sample 100g in 250ml sterilization triangular flask, add the K of 5ml 100mM
2CrO
4, stir evenly gently to put in 37 ℃ of incubators and cultivate a week, note adding sterilized water, guarantee that sample is dried.
(3) chromium resistance bacterium separates: accurately take by weighing K
2CrO
4Enrichment soil sample 10g puts in 37 ℃ of shaking tables and vibrates half an hour in the triangular flask that the 90ml stroke-physiological saline solution is housed, and gets 1ml more successively and progressively be diluted to 10 in the 9ml stroke-physiological saline solution
-4, 10
-5, 10
-6, get the K that the 0.1ml coating contains 1mM respectively
2CrO
4LB solid medium flat board, 3 flat boards of each extent of dilution coating are put in 37 ℃ of incubators and are cultivated a week, the bacterial strain that grows is a chromium resistance bacterium, puts in 4 ℃ of refrigerators flat board stand-by.LB solid culture based formulas is as follows, in the 1L solution, and Tryptones (Tryptone): 10g; Yeast extract (ctYeast Extra): 5g; Sodium-chlor (NaCl): 10g; Agar, 15g.
(4) line separates: the different bacterium colony of chromium resistance bacterium picking that obtains in the step (3) is rule on the LB solid plate, guarantee to obtain mono-clonal, treat to put after bacterium grows in 4 ℃ of refrigerators stand-by and a in-80 ℃ of refrigerators with the preservation of glycerine freeze pipe.
(5) chromium reduction bacterium screening: the chromium resistance bacterium mono-clonal that obtains in the step (4) is transferred in the 10ml LB liquid nutrient medium, adds the K of 100ul 100mM
2CrO
4, making its final concentration is 1mM, puts shaking culture in 37 ℃ of shaking tables, and timing.Beginning for some time is somatic cells vegetative period, does not almost have iuvenescence, and it enters the logarithmic growth after date by the time, and cell density increases, and rate of reduction begins to accelerate, and detects from this moment beginning cluster sampling.Sampling procedure is as follows: under aseptic condition, get 1.5ml bacterium liquid in the 2ml centrifuge tube, 12000rpm got supernatant in centrifugal 4 minutes and detects.Got one time sample every two hours.The measuring method of chromium in the supernatant (VI) all adopts the chromic mensuration-diphenyl carbazide spectrophotometry of water quality (State Bueau of Environmental Protection; State Standard of the People's Republic of China GB7467-87; Beijing; China Standard Press; November in 1987 the 1st edition); specific as follows: as to get supernatant 1ml and place the 50ml colorimetric cylinder, be diluted with water to graticule.Add 0.5ml sulphuric acid soln (1:1) and 0.5ml phosphoric acid solution (1:1), shake up.Add 2ml diphenylcarbazide developer, shake up, after 5～10 minutes, in 540nm wavelength place, water is cooked reference, measures absorbancy, checks in corresponding content of 6-valence Cr ions from typical curve.It is the content of 6-valence Cr ions that has been reduced that initial value deducts residual value shi.Under the same terms, the reductive sexavalent chrome is many more, and the reductibility of bacterium is strong more.
(6) classification of chromium reduction bacterium is identified: the one, and utilize 16S rDNA to identify, promptly adopt prokaryotic organism 16S rDNA universal primer 27F (being forward primer): 5 ' AGAGTTTGATCMTGGCTCAG3 ') and 1492R (reverse primer): 5 ' GGYTACCTTGTTACGACTT3 ' be PCR (concrete operation method of PCR be referring to applicant's granted patent document, and the patent No. is the ZL200510120584.7 denomination of invention: a kind of small quality fast extraction method for soil total DNA).Increase its 16S rDNA and order-checking, with international NCBI GenBank (www.ncbi.nlm.nih.gov) Nucleotide database comparison, nucleotide homology is 97%, is accredited as a sporangiocyst bacterium again; The 2nd, gramstaining analysis and growth characteristics are identified.
Sporangiocyst bacterium (Intrasporangiaceae sp.) Q5-1 mycology feature is as follows between the present invention's preparation:
Between sporangiocyst bacterium (Intrasporangiaceae sp.) Q5-1 be Gram-negative bacteria (the gramstaining photo is seen shown in the accompanying drawing 2), 37 ℃ of suitable growth temperatures, appropriate pH 8.0 is on the LB solid medium that little Huang is transparent, circular, the bacterium colony of projection.
Between the preservation of sporangiocyst bacterium Q5-1:
Between sporangiocyst bacterium Q5-1 can under 4 ℃, make short term storage after the cultivation 37 ℃ of cultivations on general LB liquid or solid substratum.If long-term preservation, can use glycerine freeze pipe or lyophilize pipe (referring to Zhao Bin, He Shaojiang. the microbiology experiment. first version. the .2002:202 of Science Press-205) preservation strain is proper.
The chromium of 2 sporangiocyst bacterium of embodiment Q5-1 is virgin curve also
Sporangiocyst bacterium Q5-1 mono-clonal is inoculated in the 100ml LB liquid nutrient medium between picking, puts shaking culture in 37 ℃ of shaking tables, and sampling and measuring is to cell density OD at interval
600Be about 0.15, be kept at 4 ℃ of refrigerators, be used for inoculation as kind of daughter bacteria liquid.Inoculum size with 1% is drawn 1ml in the fresh LB liquid nutrient medium of 100ml, adds the K of 1ml 100mM
2CrO
4, making its final concentration is 1mM, puts shaking culture in 37 ℃ of shaking tables.Begin to cultivate begin after 8 hours the sampling, got sample one time every 2 hours, until K
2CrO
4Fully till the reduction.Get 1.2ml and 1.5ml at every turn respectively, survey cell density (OD respectively with spectrophotometry again
600) and hexavalent chromium concentration (OD
540), all enchashment is now done.Specific practice is as follows: the one, survey cell density (OD
600), be reference with the deionized water, directly measure its light absorption value at wavelength 600nm place with the sample of 1.2ml.The 2nd, hexavalent chromium concentration (OD
540), getting 1.5ml bacterium liquid in the 2ml centrifuge tube, 12000rpm got supernatant in centrifugal 4 minutes and detects.The measuring method of chromium in the supernatant (VI) all adopts chromic mensuration diphenyl carbazide spectrophotometry (State Bueau of Environmental Protection; State Standard of the People's Republic of China GB7467-87; Beijing; China Standard Press; November in 1987 the 1st edition); concrete steps are as follows: the supernatant 1ml that gets after centrifugal places the 50ml colorimetric cylinder, is diluted with water to graticule.Add 0.5ml sulphuric acid soln (volume ratio is 1:1) and 0.5ml phosphoric acid solution (volume ratio is 1:1), shake up.Add 2ml diphenylcarbazide developer, shake up, after 5～10 minutes, in 540nm wavelength place, water is cooked reference, measures absorbancy, checks in corresponding content of 6-valence Cr ions from typical curve.Between drawing the chromium of sporangiocyst bacterium Q5-1 also virgin curve see accompanying drawing 3.
Embodiment 3: a sporangiocyst bacterium Q5-1 the simulation chromium pollution water in to the chromic reduction effect that adds up
Adopt deionized water to add K
2CrO
4Be used for simulating chromium pollution water, sporangiocyst bacterium Q5-1 is to the chromic removal effect that adds up between investigation.Specific practice is as follows: prepare three 250ml triangular flasks, as three repetitions, each adorns 100ml LB substratum (121 ℃, high pressure steam sterilization 30 minutes), and the inoculum size with 1% is drawn 1ml cell density OD
600Be the bacterium liquid inoculation of about 0.15 Q5-1, add 1ml 100mM K
2CrO
4Mother liquor, making its final concentration is 1mM, puts shaking culture in 37 ℃ of shaking tables.Begin to cultivate 12 hours beginning sampling and measuring, until the K that adds for the first time
2CrO
4Almost reduction fully; Next add 1ml 100mM K once more
2CrO
4Mother liquor, making its final concentration is 1mM, continues sampling and detects, until the K that adds for the second time
2CrO
4Almost K is for the third time added in reduction fully again
2CrO
4, the rest may be inferred, till thalline can not restore sexavalent chrome.See accompanying drawing 3 with the diphenyl carbazide spectrophotometry detected result during this time.As can be seen from the figure, can to reduce the continuous final concentration that adds for three times in 72 hours be the K of 1mM to a sporangiocyst bacterium Q5-1
2CrO
4The simulation chromium pollution water, rate of reduction is fast, repeatability better has a good application prospect.
4 sporangiocyst bacterium of embodiment Q5-1 carries out immobilization embedded to chromic reduction effect.
Adopt immobilization material that a sporangiocyst bacterium Q5-1 is carried out embedding, again the bacterium that fixes is rendered in the simulation chromium pollution water, see the effect of its reduction of hexavalent chromium.
Specific practice is as follows:
(1) on the preliminary study basis,, selects the fixation support material in conjunction with bibliographical information, be respectively polyvinyl alcohol, sodium alginate, gac, diatomite, the ratio of solid support material percentage ratio are by weight counted polyvinyl alcohol (4%)-sodium alginate (3%)-gac (1.5%)-diatomite (3%).
(2) preparation of immobilization bacterium.Preparing a volume is the 250ml triangular flask, dress 100ml LB substratum (at 121 ℃, high pressure steam sterilization 30 minutes), and the inoculum size with 1% is drawn 1ml cell density OD
600Be the inoculation of 0.15 left and right sides Q5-1 bacterium liquid, put shaking culture in 37 ℃ of shaking tables, begin to cultivate and begin sampling after 8 hours, survey its cell density (OD
600), work as OD
600Be about 0.7, just can stop to cultivate, collect thalline.The microorganism collection method is: cultured Q5-1 bacterium liquid transferred in the aseptic centrifuge tube of 50ml, and at 4 ℃, under the 12000rpm condition, centrifugal 10 minutes, the reject supernatant; The phosphoric acid buffer suspension thalline that adds 25ml pH8.0 again, 4 ℃, 12000rpm, centrifugal 10 minutes washing thalline; Use twice of phosphoric acid buffer repeated washing again; At last washed thalline is concentrated in the centrifuge tube,, places 4 ℃ of refrigerators, use when waiting until immobilization with the phosphoric acid buffer suspension of 10ml pH8.0.
(3) with each fixation support material in ratio weighing in (1), add the sterilized water constant volume to 100ml, with thermostat water bath 90 ℃ of dissolvings, after being cooled to 37 ℃, mix with bacterium concentrated solution in (2), to mix liquid with the pressurization of the syringe of 1.5mm diameter syringe needle then and be expelled to balling-up in the saturated boric acid crosslinked fluid that contains 2% calcium chloride, crosslinking time is 24 hours.After crosslinked the finishing that immobilized spherule is stand-by with the aseptic deionized water washed twice.
(4) prepare 3 250ml triangular flasks, as three repetitions, each adorns 100ml LB substratum (121 ℃, high pressure steam sterilization 30 minutes), adds 0.5ml 100mM K
2The CrO4 mother liquor, making its final concentration is 0.5mM, with immobilized spherule input in (3), puts shaking culture in 37 ℃ of beds.Begin sampling and detect the sexavalent chrome residual content, remove fully until it.
Above-mentioned experiment shows, it is better to the hexavalent chrome reduction effect that sporangiocyst bacterium Q5-1 carries out immobilization embedded back between the present invention's preparation, the wherein combination of polyvinyl alcohol (4%), sodium alginate (3%), gac (1.5%) and diatomite (3%) can be with the K of 0.5mM at 17 hours
2CrO
4Reduction 97.5%.This combination is seen accompanying drawing 4 with the hexavalent chrome reduction effect comparative effectiveness of not carrying out immobilization embedded free cell.Its reduction effect approaches free cell as seen from Figure 4, and makes automation technolo in application, and serialization is easy to use, can improve processing efficiency, reduces production costs, so have good application prospects.
Claims (2)
1, a strain can be an inorganic chromic sporangiocyst bacterium (Intrasporangiaceae) with inorganic hexavalent chrome reduction, is deposited in Chinese typical culture collection center (CCTCC), and its preserving number is M208239.
2, described sporangiocyst bacterium of claim 1 Q5-1 application aspect the pollution of chromium in purifying liquid waste.
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| CN103910437A (en) * | 2014-04-18 | 2014-07-09 | 湖南大学 | Method for removing heavy metal ions out of water |
| CN104450589A (en) * | 2014-12-25 | 2015-03-25 | 厦门大学 | Sporosarcina saromensis and screening method and application thereof |
| CN104560801A (en) * | 2014-12-25 | 2015-04-29 | 厦门大学 | Mesonia sp. as well as screening method and application thereof |
| CN107475238A (en) * | 2017-09-04 | 2017-12-15 | 华中农业大学 | A kind of immobilized microorganism of lotus pod and its preparation method and application |
| CN108753647A (en) * | 2018-06-12 | 2018-11-06 | 湖北师范大学 | A kind of immobilized bead of arsenic removal and application thereof |
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2008
- 2008-12-05 CN CN2008102366799A patent/CN101429487B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103910437A (en) * | 2014-04-18 | 2014-07-09 | 湖南大学 | Method for removing heavy metal ions out of water |
| CN103910437B (en) * | 2014-04-18 | 2015-07-15 | 湖南大学 | Method for removing heavy metal ions out of water |
| CN104450589A (en) * | 2014-12-25 | 2015-03-25 | 厦门大学 | Sporosarcina saromensis and screening method and application thereof |
| CN104560801A (en) * | 2014-12-25 | 2015-04-29 | 厦门大学 | Mesonia sp. as well as screening method and application thereof |
| CN107475238A (en) * | 2017-09-04 | 2017-12-15 | 华中农业大学 | A kind of immobilized microorganism of lotus pod and its preparation method and application |
| CN107475238B (en) * | 2017-09-04 | 2019-12-24 | 华中农业大学 | Lotus seed pot immobilized microorganism and preparation method and application thereof |
| CN108753647A (en) * | 2018-06-12 | 2018-11-06 | 湖北师范大学 | A kind of immobilized bead of arsenic removal and application thereof |
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| Publication number | Publication date |
|---|---|
| CN101429487B (en) | 2010-12-22 |
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