CN103451105B - A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application - Google Patents
A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application Download PDFInfo
- Publication number
- CN103451105B CN103451105B CN201210181161.6A CN201210181161A CN103451105B CN 103451105 B CN103451105 B CN 103451105B CN 201210181161 A CN201210181161 A CN 201210181161A CN 103451105 B CN103451105 B CN 103451105B
- Authority
- CN
- China
- Prior art keywords
- cadmium
- filamentous fungus
- heavy metal
- penicllium chrysogenum
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of filamentous fungus Penicllium chrysogenum J-5 and preparation method and application of high absorption cadmium, step is: first take 10g cadmium pollution soil in the sterile saline of 90mL, vibrate on constant-temperature table, after leaving standstill, gets supernatant liquor and dilutes; Next is coated on by the diluent of different extension rate on the Martin's rose-bengal flat board containing 10mM cadmium, is inverted in constant incubator and cultivates; 3rd is that the microorganism of grow on plates is carried out line separation and purification, is then again applied on the above-mentioned Martin's rose-bengal flat board containing cadmium and cultivates; 4th is after separation screening, preserves bacterial strain cadmium to stable resistance ability, and PDA inclined-plane is preserved.The filamentous fungus Penicllium chrysogenum J-5 obtained has height endurability and high characterization of adsorption to various heavy, and raw material easily obtains, with low cost.This bacterial strain has higher crystallized ability to the heavy metal in environment, can reduce its biological toxicity, is applicable to the in-situ immobilization of pollution in wide area soil.
Description
Technical field
The invention belongs to field of environmental improvement.More specifically relate to a kind of preparation method various heavy to the filamentous fungus of height endurability and high absorption capacity, also relate to the purposes of a kind of filamentous fungus Penicllium chrysogenum J-5 simultaneously.
Background technology
Cadmium (Cd) mainly exists with the form of 13 kinds of mineral such as greenockite at occurring in nature, and normal association is in other metallic ores, as Pb-Zn deposits etc.The mean concns of Cd in the earth's crust is 0.15mg/Kg (Yan Mingjuan, 1999), and soil Cd concentration is at 0.01-2mg/Kg, and in Different Waters, Cd concentration is 0.11-10 μ g/Kg.Although under normal circumstances, Cd exists with the state of trace in physical environment, and the activity of the mankind makes its content in the environment and distribution range expanding day.As Cd can directly enter air, water body and soil, and move in different environments, cause environmental pollution; Especially the discharge of trade effluent waste residue waste gas, causes serious environmental pollution especially.
In edatope, Cd exists with variform.According to Sposito continuous extraction (1982), the form of the cadmium in soil can be divided into exchange state, water-soluble state, organically combine state, carbonate combined, sulphide tailings state and residual form.The difference of Cd various form proportion in soil, directly affects its migration in soil, conversion, Adsorption and desorption ability, and the physiological-toxicity to biology.According to Cd, bioavailability is respectively from high to low: water-soluble state and exchange state > carbonate combined > organically combine state > residual form.Water-soluble state and exchange state Cd the strongest to bio-toxicity.In soil, the existing forms of Cd is subject to the impact of character, content, soil constitution structure (as: organic matter, carbonate, mineral and microorganism) and the envrionment conditions (as: pH, temperature and humidity etc.) of itself.Therefore, along with the change of environment, can occur between the various forms of Cd to transform, and the chemical equilibrium theory of this conversion behavior to general in certain system is similar, as the balance between acid base equilibrium, redox, adsorption-desorption, coordination balance and resolution of precipitate.But, because soil itself is a complicated system, this conversion behavior may more complicated (Liao Min etc., 1999).
In water surrounding, the main of Cd exists with ionic state cadmium.In addition, Cd can form solvend with the multiple ligands in water body, as Cd (OH
-)
+, HCdO
2 -, CdCl
+, Cd (NH
3)
2+, Cd (HCO
3)
2deng.The transfer ability of Cd in water body can be thought: ionic state > complex state > indissoluble state.When Yao Chonghua etc. (1982) research shows water surrounding generation heavy metal contamination, the increase of Cd total amount can not impact original fractions distribution except changing to some extent ADSORPTION STATE substantially to self distribution.Suspended substance in water body, settling, the factors such as hydrobiont all can affect the conversion in water body between different shape Cd.In environment Cd different shape between conversion behavior be in environment Cd pollute improvement provide certain theories integration; how effectively to reduce the biological effectiveness of Cd in environment; reduce the enrichment of Cd in agricultural-food; study hotspot (ReddyandParupudi, 1997 of field of Environment Protection both at home and abroad in the last few years; Bastaetal., 2001; Dielsetal., 2002; WeiandZhou, 2006).
Tradition is administered the method for heavy metal containing sewage and really be have received certain effect, but in the face of polluting day by day serious present situation, in the long run these methods in various degree have that cost is high, energy consumption is high, complex operation, easily cause the shortcomings such as secondary pollution.Since Ruchhoft in 1949 propose biosorption process (active sludge) remove heavy metal plutonium in sewage (
239pu) since, biological process remediating heavy metal sewage receives increasing concern, particularly utilizes the research of microbiological treatment heavy metal containing sewage to become study hotspot (KratochvilandVolesky, 1998 gradually; Volesky, 1999).
Administer in heavy metal contamination process at biological adsorption, filamentous fungus receives to be paid close attention to widely.The structure of cell surface of fungi is similar to ionic exchange resin, has potential absorption, ability (Gadd, 1993 of ion-exchange and covalent attachment heavy metal ion; KrantzR ü lckeretal., 1993; Kapooretal., 1999).Fungi can be divided into two kinds of different types for the absorption mechanism of heavy metal ion from energy metabolism angle: a kind of is the non-energy dependency of effect rapidly, mainly pass through some structures of phage surface as carboxyl, hydroxyl, physical and chemical adsorption (the Gadd that sulfydryl and phosphate group etc. carry out, 1993), another kind is then physiological metabolism dependent form slowly, as precipitated (Vodniketal., 1998) in transmembrane transport and cell.Therefore, the thalline of living and dead mycelium can with the biological adsorption agents dealing with heavy metal.Some researchs even show that dead mycelium is greater than thalline alive for the adsorptive capacity of heavy metal.Jarosz-WilkoLazka etc. (2002) have studied the adsorptive power of white-rot fungi Trametesversicolor heavy metal Cd, find that white-rot fungi has very strong adsorptive power for the Cd of liquid phase environment; Through the process of two hours, most Cd can be removed.Wherein the adsorptive capacity of energy independent form can reach ~ 2mg/ (g dry mycelium) fast, and the adsorptive capacity of energy independent is at a slow speed ~ 0.3mg/ (g dry mycelium).Malik (2004) thinks and utilizes viable cell will be more realistic to carry out the biological adsorption of heavy metal, and can show better effect.
The tolerance of fungi heavy metal or resistance have multiple different biological mechanism; as the absorption of the transformation of the precipitating action of mucilage secretion heavy metal, complexing action, crystallization effect, metal state, cell-wall component and pigment molecular heavy metal ion, the change of wall membrane permeability, (Gadd, 1993 such as active efflux mechanism and intracellular enrichment isolation; MehraandWinge, 1991).Zafar etc. (2007) screen the filamentous fungus that two strains have heavy metal resistance from the agricultural land soil of heavy metal contamination, are accredited as Aspergillus and Rhizopus.This two strains filamentous fungus has high biological adsorption ability to Cd and Cr, demonstrates the utilization prospect of potential Biosorbent for heavy metals.And Wasay more early etc. (1998) utilize Aspergillusniger can produce organic acid characteristic to process the soil (clay loam of the heavy metal contamination of three kinds of differing textures, clay, sandy soil), find the process through 20 to 25 days, the heavy metal in these three kinds of soil can be made to meet the heavy metal-polluted soil standard formulated Quebec.In general, utilize the report of fungal biodegradation heavy-metal contaminated soil few at present, fungus resource is quite limited, and ripe treatment process and method need further research and development and perfect.
Summary of the invention
The object of the invention is the filamentous fungus Penicllium chrysogenum J-5 that there are provided a kind of efficient adsorption heavy metal.The present invention has been separated a strain filamentous fungus from cadmium pollution soil.This bacterial strain in laboratory conditions can remarkable adsorbing heavy metal cadmium; Simultaneously, this filamentous fungus and Plantula Brassicae chinensis symbiosis water planting show this filamentous fungus can fix cadmium in nutrient solution by efficient adsorption, reduce its biological effectiveness, improve the output of plant and reduce the absorption of plant to cadmium, showing the wide utilization prospect of filamentous fungus provided by the present invention in heavy-metal contaminated soil and water body treating process.
Another object of the present invention is the preparation method of the filamentous fungus Penicllium chrysogenum J-5 that there are provided a kind of efficient adsorption heavy metal, this preparation method is simple and efficient, the thalline obtained has the ability of high Adsorption of Heavy Metals, and adsorption process is not by the restriction of nutritional condition.
Another object of the present invention there are provided the application of a kind of filamentous fungus Penicllium chrysogenum J-5 in the biological restoration of heavy metal-polluted water and soil.Filamentous fungus Penicllium chrysogenum J-5 can fix heavy metal by active adsorption, reduces its biological effectiveness, reach the object of administering heavy metal contamination, and it has feature with low cost, not easily to cause secondary pollution, possesses the potentiality of commercialized development.
The present invention is achieved in that
A preparation method of the filamentous fungus Penicllium chrysogenum J-5 of efficient adsorption heavy metal, its step is separation, purifying and multiple sieve.Detailed process is: first take 10g cadmium pollution soil in the sterile saline of 90mL, and on 28 DEG C of constant-temperature tables, 150r/min vibration 15min, after leaving standstill 1min, gets supernatant liquor and carry out 10 times of serial dilutions; Next is coated on the Martin's rose-bengal flat board containing 10mM cadmium by the diluent of different for 100 μ L extension rate, is inverted in 28 DEG C of constant incubators and cultivates 5d; 3rd is that the microorganism of grow on plates is carried out line separation and purification, is then again applied on the above-mentioned Martin's rose-bengal flat board containing cadmium and cultivates; 4th is after 10 separation screenings, preserves bacterial strain cadmium to stable resistance ability, PDA inclined-plane is preserved, for subsequent use.
A strain bacterium called after filamentous fungus J-5 in the bacterial strain that above-mentioned separation obtains by applicant, and be accredited as Penicllium chrysogenum (Penicilliumchrysogenum) J-5, this bacterial strain is delivered China typical culture collection center (CCTCC) preservation on April 24th, 2012 by applicant, preservation address: Wuhan, China Wuhan University, deposit number is: CCTCCNO:M2012137, Classification And Nomenclature: Penicllium chrysogenum J-5PenicilliumchrysogenumJ-5.
The characteristic of filamentous fungus Penicllium chrysogenum J-5 is as follows:
1. biological characteristics: filamentous fungus, broom shape branched structure is obvious, obligate aerobic, optimum growth temperature 20 DEG C ~ 28 DEG C; In PDA substratum, sporulation quantity is large, and the sporulation initial stage is light green, and the later stage is blackish green, and the cultivation middle and later periods has obvious xanthein to produce.
2. genetics characteristic: combining form is observed and molecular biosciences identifies that this bacterium of confirmation is Penicllium chrysogenum.
3. culture condition: the substratum used when preserving this fungi and obtain its spore is PDA substratum; For adsorption experiment and test its heavy metal tolerance mineral salts medium (MM): (NH
4)
2sO
45g/L; KH
2pO
415g/L; MgSO
40.6g/L; CaCl
20.6g/L; FeSO
47H
2o5mg/L; CoCl
22mg/L; Glucose 20g/L, pH5.0.121 DEG C of high pressure steam sterilization 30min.Cultivate this bacterium can the form of spore inoculate: rinse the spore on PDA inclined-plane with the tween-80 of 2% volume ratio, inoculate then be diluted to the spore concentration of needs with the tween-80 of 0.2% volume ratio after, and carry out 28 DEG C, 150r/min shake-flask culture.Fig. 1 is the growth curve of J-5 in mineral salt (MM) substratum, enters plateau after cultivating 5d.
3. functional performance: there is the tolerance to various heavy.Cultivate to containing in the mineral salts medium of specific heavy metal concentration by 1% inoculum size inoculation filamentous fungus J-5 spore suspension, after 7d, observe its upgrowth situation.The tolerance detected result of filamentous fungus J-5 to different heavy metal ion is: to cadmium (Cd under liquid culture condi
2+) 320mM, copper (Cu
2+) 80mM, cobalt (Co
2+) 30mM, chromium (Cr
6+) 5mM, chromium (Cr
3+) 35mM, zinc (Zn
2+) heavy metal such as 70mM has good tolerance.
The application of filamentous fungus Penicllium chrysogenum J-5 in the biological restoration of heavy metal-polluted water and soil, the steps include:
(1) static cadmium absorption:
After bacterial strain filamentous fungus J-5 is cultivated 5d on PDA substratum, collect spore, prepare spore suspension; Then be inoculated into according to the ratio of 1:100 in the fresh mineral salts medium of 200mL and cultivate 5d, collecting by filtration thalline also carries out vacuum lyophilization; Then, after dry bacterial powder being crossed the sieve in 300 μm of apertures, for adsorbing the analysis of cadmium.
By 0.01g through above-mentioned pretreated bacterium powder, join in the 50mL centrifuge tube containing 20mL cadmium solution, then centrifuge tube is placed in 28 DEG C of constant-temperature tables, 150r/min maintains 240min.Respectively 0 in adsorption process, 15,30,45,60,120,180 and 240min get 100 μ L supernatant liquors, carry out cadmium content analysis after dilution.
(2) heavy metal out of electroplating wastewater removes experiment:
Inventors tested a large filamentous fungus J-5 to heavy-metal movement removing solid capacity in actual electrical waste electroplating.Adopt the treatment process that embodiment 2.1 is identical, prepare dry bacterial powder.The dry bacterial powder of 0.01g is joined the electroplating wastewater (Cd of 20ml
2+: 0.13mg/L; Cu
2+: 0.15mg/L) in, 28 DEG C, collecting by filtration supernatant liquor after 150r/min process 2h, measures the concentration of Cadmium In The Water Body before and after absorption according to preceding method.
(3) filamentous fungus Penicllium chrysogenum J-5 and plant symbiosis solution culture:
The present embodiment is intended to prove the protective capability of filamentous fungus J-5 provided by the present invention to plant-growth in simulation heavy-metal contaminated soil.The plantation of Plantula Brassicae chinensis and cultural method are see document (Green, 1989; Yu Fangming etc., 2008); The treatment process of filamentous fungus J-5 dry bacterial powder is as above as described in embodiment 2.Before inoculation filamentous fungus J-5 to hydroponic culture systems, collect filamentous fungus J-5 spore and regulate spore suspension to 10
7individual/mL, for subsequent use.
With Plantula Brassicae chinensis (BrassicachinensisL.) for test plant, get the consistent seed of 10 Germinating status after vernalization in the planting cup that 100g sterilizing vermiculite is housed.After emerging, every 2d waters 10mL sterile tap water, changes Hoagland ' the s nutritive medium (Yu Fangming etc., 2008) of 0.5 times of concentration after 5d, grows after 2 true leaves start to water pancebrin until Plantula Brassicae chinensis.Thinning to 4 strain after cultivation 10d, (final concentration is 10 then to add 100 μ LJ-5 spore suspensions respectively according to Different treatments
4individual/g vermiculite) and the freeze-drying J-5 thalline of 0.01g.After 2d, in training sample system, add different Hoagland ' the s nutritive medium of 10mL cadmium content respectively, make it join the final concentration of cadmium in culture system and be respectively 0,0.1,0.5,1 and 2mg/Kg vermiculite.The nutritive medium containing cadmium is no longer added in follow-up culturing process, but every 2d Additional nutrient solution, ensure water-content substantially constant.Plant is gathered in the crops after continuing to cultivate 30d; By the blade-section of the plant of results, after 65 DEG C of oven dry 48h to constant weight, get 0.01g and carry out digestion process in 1mL concentrated nitric acid, after dilute filtration, measure cadmium content.Above-mentioned pot experiment is often planted process and is arranged 4 repetitions, and whole test completes in illumination box, illumination 14h, temperature 28 DEG C, relative humidity 60%.
Therefore, the present invention can be summarized as: applicant, from cadmium pollution soil, utilizes fungus culture method, carries out fungi screening; Obtain a strain and can contain the filamentous fungus that Martin's rose bengal medium of 10mM cadmium grows, applicant is by its called after J-5.This filamentous fungus is Penicllium chrysogenum, and preservation and the product Spore cultivation base of this bacterial strain are PDA substratum.
Applicant utilizes filamentous fungus J-5 to carry out the absorption of static cadmium and heavy metal out of electroplating wastewater removes experiment, and showing this bacterial strain can rapidly and efficiently adsorbing heavy metal cadmium.The symbiosis solution culture of the J-5 carried out subsequently and plant shows this bacterial strain and can reduce the absorption of plant to cadmium, increases the output of Plantula Brassicae chinensis.
The present invention compared with prior art, has the following advantages and effect:
(1) thalline raw material easily obtains, with low cost.
(2) utilize the freeze-drying thalline of filamentous fungus Penicllium chrysogenum J-5 not only can remove heavy metal ion in sewage by efficient adsorption as biological adsorption agent, also possess good adsorbent reactivation ability simultaneously, have the potential of commercial applications.
(3) filamentous fungus Penicllium chrysogenum J-5 has height endurability and high characterization of adsorption to various heavy, has higher crystallized ability to the heavy metal in environment, can obviously reduce its biological toxicity.This bacterial strain can be survived under high density heavy metal level, therefore can realize using Penicllium chrysogenum J-5 as soil heavy metal passivant to heavy metal in soil long-term immobilized while, avoid the passivator repeatedly applying heavy metal in environment, possess the potentiality of in-situ immobilization pollution in wide area soil.
Accompanying drawing explanation
Fig. 1 is a kind of filamentous fungus J-5 growth curve measurement result in mineral salts medium.
Fig. 2 is the static cadmium adsorptive capacity measurement result of a kind of filamentous fungus J-5.
Fig. 3 is that a kind of filamentous fungus J-5 is to reality plating sewage disposal result.
Fig. 4 be a kind of filamentous fungus J-5 by different way with the impact of plant symbiosis water planting on plant biomass.
In figure: CK indicates experiment condition for not add any type of J-5; Dead freeze-drying J-5 is added in DJ instruction; LJ indicates interpolation
J-5 spore.
Fig. 5 be a kind of filamentous fungus J-5 by different way with the impact of plant symbiosis water planting on plant leaf cadmium content.
In figure: CK indicates experiment condition for not add any type of J-5; Dead freeze-drying J-5 is added in DJ instruction; LJ indicates interpolation
J-5 spore.
Embodiment
Below describing is embodiment according to embodiments of the present invention.Should be noted that embodiments of the invention only have illustration for the present invention, and there is no restriction.Other various experimental implementation involved in the present invention, be the ordinary skill in the art, the part be not particularly illustrated in literary composition, those of ordinary skill in the art can be implemented with reference to the various common tool books before the present patent application day, scientific and technical literature or relevant specification sheets, handbook etc.
Embodiment 1: the separation of filamentous fungus J-5 and the test of heavy metal tolerance
1, strains separation:
Take 10g cadmium pollution soil in the sterile saline of 90mL, on 28 DEG C of constant-temperature tables, 150r/min vibration 15min, after leaving standstill 1min, gets supernatant liquor and carries out 10 times of serial dilutions; Then the diluent of different for 100 μ L extension rate is coated on the Martin's rose-bengal flat board containing 10mM cadmium, is inverted in 28 DEG C of constant incubators and cultivates 5d.The microorganism of grow on plates is carried out line separation and purification, is then again applied on the above-mentioned Martin's rose-bengal flat board containing cadmium and cultivates.After 10 separation screenings, preserve bacterial strain cadmium to stable resistance ability, PDA inclined-plane is preserved, for subsequent use.
A strain bacterium called after filamentous fungus J-5 in the bacterial strain that above-mentioned separation obtains by applicant, penicillium chrysogenum (Penicilliumchrysogenum), this bacterial strain is delivered China typical culture collection center (CCTCC) preservation on April 24th, 2012 by applicant, and its deposit number is: CCTCCNO:M2012137.
2, the biology of bacterial strain:
1. biological characteristics: filamentous fungus, broom shape branched structure is obvious, obligate aerobic, optimum growth temperature 20 DEG C ~ 28 DEG C; In PDA substratum, sporulation quantity is large, and the sporulation initial stage is light green, and the later stage is blackish green, and the cultivation middle and later periods has obvious xanthein to produce.
2. genetics characteristic: combining form is observed and molecular biosciences identifies that this bacterium of confirmation is Penicllium chrysogenum.
3. culture condition: the substratum used when preserving this fungi and obtain its spore is PDA substratum; For adsorption experiment and test its heavy metal tolerance mineral salts medium (MM): (NH
4)
2sO
45g/L; KH
2pO
415g/L; MgSO
40.6g/L; CaCl
20.6g/L; FeSO
47H
2o5mg/L; CoCl
22mg/L; Glucose 20g/L, pH5.0.121 DEG C of high pressure steam sterilization 30min.Cultivate this bacterium can the form of spore inoculate: rinse the spore on PDA inclined-plane with the tween-80 of 2% volume ratio, inoculate then be diluted to the spore concentration of needs with the tween-80 of 0.2% volume ratio after, and carry out 28 DEG C, 150r/min shake-flask culture.Fig. 1 is the growth curve of J-5 in mineral salt (MM) substratum, enters plateau after cultivating 5d.
3, the heavy metal tolerance test of J-5:
Cultivate to containing in the mineral salts medium of specific heavy metal concentration by 1% inoculum size inoculation filamentous fungus J-5 spore suspension, after 7d, observe its upgrowth situation.The tolerance detected result of filamentous fungus J-5 to different heavy metal ion is: to cadmium (Cd under liquid culture condi
2+) 320mM, copper (Cu
2+) 80mM, cobalt (Co
2+) 30mM, chromium (Cr
6+) 5mM, chromium (Cr
3+) 35mM, zinc (Zn
2+) heavy metal such as 70mM has good tolerance.
Embodiment 2: filamentous fungus Penicllium chrysogenum J-5 cadmium adsorptive power is tested
2.1 static cadmium absorption:
After bacterial strain filamentous fungus J-5 is cultivated 5d on PDA substratum, collect spore, prepare spore suspension; Then be inoculated into according to the ratio of 1:100 in the fresh mineral salts medium of 200mL and cultivate 5d, collecting by filtration thalline also carries out vacuum lyophilization; Then, after dry bacterial powder being crossed the sieve in 300 μm of apertures, for adsorbing the analysis of cadmium.
By 0.01g through above-mentioned pretreated bacterium powder, join in the 50mL centrifuge tube containing 20mL cadmium solution, then centrifuge tube is placed in 28 DEG C of constant-temperature tables, 150r/min maintains 240min.Respectively 0 in adsorption process, 15,30,45,60,120,180 and 240min get 100 μ L supernatant liquors, carry out cadmium content analysis after dilution.Adsorption experiment arranges 3 repetitions.The analysis of cadmium content adopts sampling Graphite Furnace Atomic Absorption spectrophotometer to carry out measuring (Sun Xining etc., 2007).As shown in Figure 2, along with the increase of adsorption time, the unit adsorptive capacity of thalline to cadmium increases gradually.Initial 45min internal adsorption amount reach to maximal absorptive capacity more than 75%, progress into adsorption equilibrium subsequently.Wherein, filamentous fungus J-5 dry powder reaches 87.31 ± 2.04mg/g(dry weight to the adsorptive capacity of cadmium in 500mg/L cadmium solution).
2.2 heavy metal out of electroplating wastewaters remove experiment:
Inventors tested a large filamentous fungus J-5 subsequently to heavy-metal movement removing solid capacity in actual electrical waste electroplating.Adopt the treatment process that embodiment 2.1 is identical, prepare dry bacterial powder.The dry bacterial powder of 0.01g is joined the electroplating wastewater (Cd of 20ml
2+: 0.13mg/L; Cu
2+: 0.15mg/L) in, 28 DEG C, collecting by filtration supernatant liquor after 150r/min process 2h, measures the concentration of Cadmium In The Water Body before and after absorption according to preceding method.As can be seen from Figure 3, the remove ability of filamentous fungus J-5 to cadmium is 85.8 ± 2.06%, is 61.7 ± 1.51% to the ability that removes of copper.Experimental result shows that the filamentous fungus J-5 in the present invention can adsorb the heavy metal cadmium removed in actual electrical waste electroplating efficiently, also has simultaneously higher remove ability to copper.
Embodiment 3: filamentous fungus J-5 and plant symbiosis solution culture
The present embodiment is intended to prove the protective capability of filamentous fungus J-5 provided by the present invention to plant-growth in simulation heavy-metal contaminated soil.The plantation of Plantula Brassicae chinensis and cultural method are see document (Green, 1989; Yu Fangming etc., 2008); The treatment process of filamentous fungus J-5 dry bacterial powder is as above as described in embodiment 2.Before inoculation filamentous fungus J-5 to hydroponic culture systems, collect filamentous fungus J-5 spore and regulate spore suspension to 10
7individual/mL, for subsequent use.
With Plantula Brassicae chinensis (BrassicachinensisL.) for test plant, get the consistent seed of 10 Germinating status after vernalization in the planting cup that 100g sterilizing vermiculite is housed.After emerging, every 2d waters 10mL sterile tap water, changes Hoagland ' the s nutritive medium (Yu Fangming etc., 2008) of 0.5 times of concentration after 5d, grows after 2 true leaves start to water pancebrin until Plantula Brassicae chinensis.Thinning to 4 strain after cultivation 10d, (final concentration is 10 then to add 100 μ LJ-5 spore suspensions respectively according to Different treatments
4individual/g vermiculite) and the freeze-drying J-5 thalline of 0.01g.After 2d, in training sample system, add different Hoagland ' the s nutritive medium of 10mL cadmium content respectively, make it join the final concentration of cadmium in culture system and be respectively 0,0.1,0.5,1 and 2mg/Kg vermiculite.The nutritive medium containing cadmium is no longer added in follow-up culturing process, but every 2d Additional nutrient solution, ensure water-content substantially constant.Plant is gathered in the crops after continuing to cultivate 30d; By the blade-section of the plant of results, after 65 DEG C of oven dry 48h to constant weight, get 0.01g and carry out digestion process in 1mL concentrated nitric acid, after dilute filtration, measure cadmium content.Above-mentioned pot experiment is often planted process and is arranged 4 repetitions, and whole test completes in illumination box, illumination 14h, temperature 28 DEG C, relative humidity 60%.
Experimental result shows, the plant adding cadmium all shows growth retardation and the short and small phenomenon of plant.As shown in Figure 4, cadmium concentration be 1mg/Kg vermiculite and 2mg/Kg vermiculite time, compared with CK process, the yield of pakchoi through J-5 freeze-drying thalline and the process of J-5 spore significantly improves (p<0.05).As seen from Figure 5, under different cadmium pollution degree, inoculation J-5 spore makes the absorption of Plantula Brassicae chinensis to cadmium reduce 1.2%-63.6%.This shows that active filamentous fungus J-5 effectively reduces the biological effectiveness of cadmium and the toxicity to plant thereof, and then yield of pakchoi is improved.
The result of above-mentioned case study on implementation illustrates in the present invention that the filamentous fungus J-5 being separated acquisition has higher adsorptive power to cadmium, reaches the object of the biological effectiveness reducing cadmium.
Claims (3)
1. a filamentous fungus Penicllium chrysogenum, is characterized in that: described bacterial strain be penicillium chrysogenum (
penicilliumchrysogenum) J-5, deposit number is CCTCCNO:M2012137.
2. the application of filamentous fungus Penicllium chrysogenum J-5 according to claim 1 in the biological restoration of cadmium or Cu polluted water.
3. the application of filamentous fungus Penicllium chrysogenum J-5 according to claim 1 in the biological restoration of cadmium or copper polluted soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210181161.6A CN103451105B (en) | 2012-06-04 | 2012-06-04 | A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210181161.6A CN103451105B (en) | 2012-06-04 | 2012-06-04 | A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103451105A CN103451105A (en) | 2013-12-18 |
| CN103451105B true CN103451105B (en) | 2015-11-25 |
Family
ID=49733947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210181161.6A Active CN103451105B (en) | 2012-06-04 | 2012-06-04 | A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103451105B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805021A (en) * | 2015-05-15 | 2015-07-29 | 湖南省农业生物技术研究中心 | Cadmium-tolerant penicillium and separation method thereof |
| CN106242074A (en) * | 2016-08-22 | 2016-12-21 | 湖南农业大学 | Penicillium chrysogenum ferrihydrite aggregation and its preparation method and application |
| CN108486031A (en) * | 2017-11-29 | 2018-09-04 | 安徽科技学院 | A kind of acclimation method of quick raising filamentous fungi heavy metal tolerance |
| CN110564624B (en) * | 2019-08-13 | 2023-01-24 | 内蒙古世洪农业科技有限公司 | High-salt-and-alkali-resistance penicillium chrysogenum and separation method and application thereof |
| CN113549613A (en) * | 2021-06-24 | 2021-10-26 | 沈阳化工大学 | Preparation and application method of biological purifying agent for removing composite pollutants in sediments |
| CN116286394B (en) * | 2023-02-13 | 2023-12-15 | 安徽农业大学 | Penicillium chrysogenum, microbial agent and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1704470A (en) * | 2004-06-02 | 2005-12-07 | 广西大学 | High metal-salt-resistant micro penicillium purpurogerum |
| CN102151551A (en) * | 2011-01-25 | 2011-08-17 | 湖南大学 | Heavy metal biological absorbent, preparation method thereof and application in treating cadmium-containing wastewater |
-
2012
- 2012-06-04 CN CN201210181161.6A patent/CN103451105B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1704470A (en) * | 2004-06-02 | 2005-12-07 | 广西大学 | High metal-salt-resistant micro penicillium purpurogerum |
| CN102151551A (en) * | 2011-01-25 | 2011-08-17 | 湖南大学 | Heavy metal biological absorbent, preparation method thereof and application in treating cadmium-containing wastewater |
Non-Patent Citations (5)
| Title |
|---|
| Accumulation of Cadmium, Lead, and Nickel by Fungaland Wood Biosorbents;Z. R. HOLAN等;《Applied Biochemistry and Biotechnology》;19951231;第53卷;136-141页，表1-2，图1-4 * |
| Cadmium(II) removal from aqueous solutions by pre-treated biomass of marine alga Padina sp.;Pairat Kaewsarn等;《Environmental Pollution》;20011231;第112卷;209-213 * |
| Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco;L. Ezzouhri等;《African Journal of Microbiology Research》;20090228;第3卷(第2期);035-048 * |
| Improvement of heavy metal biosorption by mycelial dead biomasses (Rhizopus arrhizus, Mucor miehei and penicillium chrysogenum):pH control and cationic activation;Eric Fourest等;《FEMS Microbiology Reviews》;19941231;第14卷;325-332 * |
| 生物吸附法去除重金属离子的研究进展;王建龙 等;《环境科学学报》;20100430;第30卷(第4期);673-701 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103451105A (en) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103451105B (en) | A kind of filamentous fungus Penicllium chrysogenum J-5 of high absorption cadmium and preparation method and application | |
| CN106493167B (en) | Bacillus licheniformis and microbial inoculum and their application and heavy metal-passivated method | |
| CN105670980B (en) | A kind of application of the microbial strains of restoration of soil polluted by heavy metal | |
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| CN101870958B (en) | Paenibacillus and application thereof to environmental engineering | |
| CN103045579B (en) | Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same | |
| CN104450569B (en) | The ultrahigh concentration bacterial strain of resistance to cadmium and its separating screening method in a kind of mining soil | |
| CN105013815B (en) | The biological renovation method of polycyclic aromatic hydrocarbon heavy-metal composite pollution soil | |
| CN103451103B (en) | High-cadmium-adsorption filamentous fungus Paecilomyces lilacinus XLA, and preparation method and application thereof | |
| CN104673715B (en) | There is fixed effect to cadmium and enterobacteria and its application of plant growth can be promoted | |
| CN110078220B (en) | Method and strain for in-situ remediation of arsenic-polluted high-saline water by using blue-green algae | |
| CN104531585A (en) | Delftia tsuruhatensis and application thereof | |
| CN112592855B (en) | Bacillus subtilis and method for treating uranium and cadmium polluted water body by using same | |
| CN106591156A (en) | Epicoccum nigrum FXZ2 and application thereof | |
| CN116948882B (en) | Achromobacter madepuralensis and application thereof | |
| CN106011039B (en) | One plant degradation terramycin amino bacillus and its application | |
| CN106544306A (en) | One plant of siderophore Producing Strain and its application in terms of farmland pollution heavy metal-polluted soil reparation | |
| CN111117909B (en) | Strain capable of resisting multiple heavy metals and promoting plant growth and application thereof | |
| CN106399176A (en) | Paenibacillus and its application in water body purification | |
| CN105505395B (en) | A kind of chitin oligosaccharide heavy metal biological renovation agent and the preparation method and application thereof | |
| CN107151663B (en) | Immobilized microbial agent prepared from kelp residue and used for petroleum pollution remediation | |
| CN106916765A (en) | A kind of method that utilization penicillium janthinellum adsorbs heavy metal in waste water zinc | |
| CN101063097A (en) | Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof | |
| Luo et al. | Unveiling the performance of a novel alkalizing bacterium Enterobacter sp. LYX-2 in immobilization of available Cd | |
| CN103740593B (en) | A kind of anabena and application thereof of energy degradation of polychlorinated biphenyl |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |