CN108486031A

CN108486031A - A kind of acclimation method of quick raising filamentous fungi heavy metal tolerance

Info

Publication number: CN108486031A
Application number: CN201711221525.8A
Authority: CN
Inventors: 祝嫦巍; 孙玉军; 黄守程; 汪雁
Original assignee: Anhui University of Science and Technology
Current assignee: Anhui University of Science and Technology
Priority date: 2017-11-29
Filing date: 2017-11-29
Publication date: 2018-09-04

Abstract

The invention discloses a kind of acclimation methods of quick raising filamentous fungi heavy metal tolerance, comprise the steps of successively：1）By the culture dish after the sterilizing of different diameter specifications（6~20cm）It nests together, bottom contacts and these culture dishes are close together on one side successively；2）The culture medium containing heavy metal of the different dense gradients of configuration, is attached separately in different culture dishes so that heavy metal concentration gradually increases from inside to outside, and culture medium liquid level is slightly below culture dish edge；3）Filamentous fungi is seeded in the culture dish of innermost layer, due to growing space limitation, mycelia is forced one direction growth and gradually undergoes the heavy metal stress pressure of higher concentration, and then improves the heavy metal tolerance of mycelia.Method using the present invention can quickly improve filamentous fungi heavy metal tolerance.

Description

A kind of acclimation method of quick raising filamentous fungi heavy metal tolerance

Technical field

The present invention relates to a kind of acclimation methods of fungi heavy metal tolerance, particularly a kind of quickly to improve filiform very The acclimation method of bacterium heavy metal tolerance.

Background technology

Due to activity such as mining, industry manufacture, exhaust gas discharge, the use of pesticide and chemical fertilizer of the mankind, cause in environment Content of beary metal increases.The environmental degradation of heavy metal pollution is directly detrimental to health, thus environmental pollution carry out prevention and It repairs most important.Carrying out heavy metal environment remediation using microorganism in recent years becomes hot spot, and filamentous fungi is in remediating heavy metal There is higher research application value in terms of contaminated soil and waste water.The effects that filamentous fungi is by intracellular accumulation, extracellular complexing, Heavy metal ion enrichment in environment to intraor extracellular is reached into reparation ring to reduce the heavy metal ion content in environment The purpose in border.Microorganism for repairing heavy metal pollution environment will not only have faster growth ability, while will be to a huge sum of money Belonging to ion has stronger tolerance and enrichment power.New bacterial strain, which is screened, from nature seeks that difficulty is big, low separation efficiency.Therefore Artificial bacterial strain of the domestication with potential using value, for improving the efficiency of research work and being repaiied in Heavy-metal Polluted Environment Practical application in multiple has important social effect and economic value.

Invention content

The purpose of patent of the present invention is to solve to improve filamentous fungi heavy metal tolerance energy in scientific research and production practices Power provides a kind of novel acclimation method, solves to screen new bacterial strain from nature at present that seek difficulty big, low separation efficiency Situation.

Patent of invention is achieved through the following technical solutions：By the culture dish after the sterilizing of different diameter specifications（6~20cm） It nests together, bottom contacts and these culture dishes are close together on one side successively（Section altogether）.The different dense gradients of configuration Culture medium containing heavy metal is attached separately in different culture dishes so that heavy metal concentration gradually increases from inside to outside, culture medium Liquid level is slightly below culture dish edge（See attached drawing 1）.

Filamentous fungi is seeded in the culture dish of innermost layer, since growing space limits, mycelia is forced one direction growth, Gradually undergo the heavy metal stress pressure of higher concentration（See attached drawing 2）.

Using the effect of above-mentioned design scheme：By the implementation of above scheme, filamentous fungi heavy metal can be quickly improved Tolerance.

Description of the drawings

Fig. 1 shows that the culture dish of different size nests together；Fig. 2 shows that filamentous fungi is seeded in heavy metal Growing state in culture medium.

Specific implementation mode

Method therefor is conventional method unless otherwise instructed in following embodiments.

Each 1 of the culture dish of 6cm, 9cm, 15cm and 20cm is taken first, autoclave sterilization processing is then carried out, ultra-clean The culture dish of the above specification is nested together in workbench, bottom contacts successively.Then it is injected in internal layer culture dish low dense Heavy metal culture dish is spent, gradually the heavy metal culture dish of high concentration is injected in outer layer difference culture dish successively, heavy metal concentration Gradient gradually increases from inside to outside.

It with the mycelium of a small amount of filamentous fungi of transfer needle picking, is inoculated in the culture dish of innermost layer, is then trained with 20cm The lid for supporting ware covers entire culture dish, is placed on the growing state that fungi is observed in 28 DEG C of incubator.When fungi adapts to After the heavy metal of higher concentration, it will continue to outgrowth.

Implementation result：Through above-mentioned acclimation method, the ability of the heavy metal tolerance of filamentous fungi is significantly improved, and the time It is short.

Claims

1. a kind of acclimation method of quick raising filamentous fungi heavy metal tolerance, it is characterised in that following steps：1）It will not With the culture dish after the sterilizing of diameter specifications（6~20cm）It nests together, bottom contacts and makes these culture dishes on one side successively It is close together；2）The culture medium containing heavy metal of the different dense gradients of configuration, is attached separately in different culture dishes so that heavy metal Concentration gradually increases from inside to outside, and culture medium liquid level is slightly below culture dish edge；3）Filamentous fungi is seeded in the training of innermost layer Support in ware, since growing space limits, mycelia be forced folk prescription grow up and the heavy metal stress pressure that gradually undergoes higher concentration Power.

2. a kind of acclimation method of quick raising filamentous fungi heavy metal tolerance described in accordance with the claim 1, feature It is：The sterilizes culture dish of different diameter specifications is nested together on one side（Section altogether）, opening is unanimously.

3. a kind of acclimation method of quick raising filamentous fungi heavy metal tolerance described in accordance with the claim 1, feature It is：Heavy metal concentration gradually increases from inside to outside in culture medium.