CN108546649A - Chlorella W2 and the application of the heavy metal in high content of beary metal water body can be removed - Google Patents
Chlorella W2 and the application of the heavy metal in high content of beary metal water body can be removed Download PDFInfo
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- CN108546649A CN108546649A CN201810468898.3A CN201810468898A CN108546649A CN 108546649 A CN108546649 A CN 108546649A CN 201810468898 A CN201810468898 A CN 201810468898A CN 108546649 A CN108546649 A CN 108546649A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
- C12N1/125—Unicellular algae isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
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Abstract
The present invention relates to a kind of chlorella that can remove the heavy metal in high content of beary metal water body, the chlorella is preserved in China typical culture collection center on November 27th, 2017, and preserving number is CCTCC NO:M2017740.The chlorella W2 of the present invention has the heavy metal ion in culture water body the tolerance of height, when being grown in the water body containing high content of beary metal, there is very high removal rate to Cu, Zn, Ni, Co, Cr, Pb, far above the microalgaes for removing removing heavy metals most of at present, it is therefore particularly suitable for the processing higher polluted-water of content of beary metal.In addition to this, while removing removing heavy metals, chlorella W2 of the invention can also remove the nutriments such as N, P in water body, prevent the danger of body eutrophication.
Description
Technical field
The present invention relates to microdisk electrodes and sewage treatment field, more specifically it relates to which a kind of can remove high content of beary metal
The chlorella of heavy metal in water body and its application.
Background technology
The heavy metal amount of the mankind to environmental emission is increasing, not only seriously polluted soil and water body environment, also gives people
The health of class itself causes great harm.To heavy metal pollution water body treating, traditional method mainly has physics, chemistry and life
Object space method, including the precipitation method, chelating resin method, polymer chelating agent method, natural zeolite absorption method, membrane technology, activated carbon adsorption
Technique, ion-exchange etc..These method clean-up effects are relatively good, but technological process is complicated, cumbersome, of high cost, and
And be not suitable for large-scale application.
Microalgae is the microorganism of generally existing in various water bodys, and breeding is fast, adaptable, need to be only inoculated in water body few
The microalgae mother liquor of amount, after breeding a period of time in suitable environment, the amount of microalgae is with regard to dense needed for attainable treated sewage
Degree.Contain abundant nitrogen and phosphorus in sewage, good inorganic nutrients are provided for micro-algae propagation.In addition, microalgae can utilize it is photosynthetic
It acts on to obtain carbon source and energy, ensures the growth of microalgae without being additionally provided carbon source, reduce cost.Therefore, use is micro-
Algae treated sewage is a kind of not only good but also economical and practical sewage treatmentmethod of effect.
Studies have shown that some microalgaes have fixed function, process to be broadly divided into physical absorption and chemistry heavy metal ion
Absorption.By microalgae be applied to sewage in heavy metal fix, while can also reduce the nutrition contents such as the nitrogen phosphorus in water body with
And other harmful substances in absorption water body, it is that one kind is achieved many things at one stroke, economical and effective sewage water treatment method.However, very
Although more microalgaes have certain heavy metal metal ion adsorption capacity, but current microalgae is to the adsorption efficiency of heavy metal ion
It is limited, also, many microalgaes are also bad to the tolerance of heavy metal ion, and which greatly limits microalgae answering in sewage disposal
With.
It being resistant to high content of beary metal and the microalgae of energy efficient absorption heavy metal ion therefore, it is necessary to a kind of.
Invention content
Inventor is from field acquisition algae sample, through culture of isolated, obtain some heavy metals that one plant can remove in environment from
The algae strain of son.Its cell has core at round or ellipse, green, atrichia.Its 18S rDNA sequence such as SEQ is obtained through sequencing
ID NO:Shown in 1, combining form is it is found that it belongs to Chlorophyta Chlorella, therefore is named as chlorella W2, Classification system
Chlorella sp.W2。
It is found based on above, the present invention provides a kind of beads that can remove the heavy metal in high content of beary metal water body
Algae, the chlorella are preserved in China typical culture collection center on November 27th, 2017, and preserving number is CCTCC NO:
M2017740。
The present invention also provides application of the above-mentioned chlorella in the heavy metal in removing high content of beary metal water body.
In one embodiment, the heavy metal is one or more combinations in Cu, Zn, Ni, Co, Cr, Pb.
The present invention also provides a kind of methods of the high content of beary metal sewage of processing comprising handles institute with above-mentioned chlorella
State the step in sewage.
In one embodiment, it the described method comprises the following steps：
S1：The sewage is filtered, large particulate matter is removed；
S2：It is inoculated with the chlorella into filtered sewage, obtains chlorella-sewage culture；
S3：The chlorella-sewage culture is subjected to blowing air illumination cultivation；
S3：After culture 8-15 days, the chlorella in the chlorella-sewage culture is removed.
In a preferred embodiment, the inoculum density of chlorella is OD in S2700Value is 0.1-0.5.
In a preferred embodiment, the condition cultivated described in S3 is 30 DEG C, 80 ± 5 μm of ol m-2s-1Illumination is strong
Degree.
The chlorella W2 of the present invention has the heavy metal ion in culture water body the tolerance of height, is containing a high huge sum of money
When being grown in the water body of category content, there is very high removal rate to Cu, Zn, Ni, Co, Cr, Pb, is used for far above most of at present
The microalgae for removing removing heavy metals is therefore particularly suitable for the processing higher polluted-water of content of beary metal.In addition to this, in removal weight
While metal, chlorella W2 of the invention can also remove the nutriments such as N, P in water body, prevent the danger of body eutrophication
Danger.
Microbial preservation
The water sample of the microalgae being related to from field acquisition of the present invention is isolated, reflects through 18S rDNA sequencings and morphology
Fixed, which belongs to Chlorophyta Chlorella.The microalgae has been preserved on November 27th, 2017 to be sent to Wuhan City, Hubei Province force
The China typical culture collection center (CCTCC) of No. 299 Wuhan Universitys of prosperous area's Bayi Road in the school, preserving number are CCTCC NO:
M2017740, is named as chlorella W2, and Classification system is Chlorella sp.W2.
Description of the drawings
Fig. 1 is the light micrograph of the chlorella W2 of the present invention；
Fig. 2 is the life that chlorella W2 is cultivated in the BG11 culture mediums in BG11 culture mediums and added with heavy metal respectively
Long curve；
Fig. 3 is that control algae chlorella G32 is trained in the BG11 culture mediums in BG11 culture mediums and added with heavy metal respectively
Support obtained growth curve.
Specific implementation mode
Principles and features of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
1. the acquisition of chlorella W2
The chlorella W2 (Chlorella sp.W2) of the present invention is isolated by the algae-containing water sample from field acquisition,
Cellular morphology is as shown in Figure 1, cell is at round or ellipse, and green, atrichia has core.It, should through 18S rDNA combining forms
Microalgae belongs to Chlorophyta Chlorella.The microalgae is preserved in Wuhan University of Wuhan City of Hubei China province on November 27th, 2017
China typical culture collection center (CCTCC), preserving number be CCTCC NO:M2017740 is named as chlorella W2, Latin
Scientific name Chlorella sp.W2.
2. chlorella W2 is to the tolerance of heavy metal
After chlorella W2 is activated in small triangular flask, it is inoculated in the BG11 cultures containing high content of beary metal equipped with 300ml
In the 500ml conical flasks of base, initial OD700It is 0.1, condition of culture is 30 DEG C, 80 ± 5 μm of ol m-2s-1It ventilates and cultivates under illumination.
BG11 culture mediums containing high content of beary metal are prepared by the way that heavy metal mother liquor is added in BG11 culture mediums.Its
In, BG11 culture mediums are prepared by the following method：Take Stock II, Stock III, Stock IV, Stock VI,
Each 1ml of Stock VII, Stock VIII, adds 1.5g NaNO3, water is added to be settled to 1000ml.Each liquid storage ingredient is as shown in table 1,
The ingredient of A5 is as shown in table 2.
The ingredient of each liquid storage during 1 BG11 culture mediums of table are prepared
2 A5 ingredients of table
The addition of heavy metal according to《Water environment quality standard》(GB3838-2002) V class (heavily contaminated) water quality mark in
It is accurate and《Urban wastewater treatment firm pollutant emission standard》To be arranged.Heavy metal mother liquor is arranged to 2000 ×.Table 3 is heavy metal
Mother liquid concentration and working concentration.
3 heavy metal mother liquid concentration of table and working concentration
After inoculation, the OD of chlorella culture is detected daily700Value, is fabricated to growth curve, with BG11 medium cultures
Chlorella be control, observation chlorella W2 is to the tolerance of heavy metal.
The results are shown in Figure 2, the growth curve that chlorella W2 is cultivated in BG11 culture mediums with added with heavy metal
The growth curve cultivated in BG11 culture mediums is not much different, it is seen that chlorella W2 is relatively high to the tolerance of heavy metal.Fig. 3 is hair
Another chlorella G32 that a person of good sense is separated in the separation process BG11 trainings in BG11 culture mediums and added with heavy metal respectively
The growth curve cultivated in base is supported, the results show that it is as known most of green algas, in the BG11 added with heavy metal
Growth in culture medium is greatly inhibited.
3. chlorella W2 is to the removal efficiency of heavy metal in culture medium
After chlorella W2 grows into stationary phase, frustule is collected by centrifugation in 6000g, is used in combination ICP-MS to detect residual in culture solution
Remaining heavy metal ion.The results are shown in Table 4, and chlorella W2 has Cu, Zn, Ni, Co, Cr, Pb very high removal rate, remote high
In the microalgaes for removing removing heavy metals most of at present, it is therefore particularly suitable for the processing higher polluted-water of content of beary metal.
4 chlorella W2 of table is to heavy metal in culture medium
4. chlorella W2 handles the trade effluent application of high content of beary metal
The trade effluent of high content of beary metal is filtered, the large particulate matter in sewage is removed.Then into sewage
It is inoculated with chlorella W2, inoculum density OD700To 0.1-0.5.At 30 DEG C, 80 ± 5 μm of ol m-2s-17-15 is cultivated under intensity of illumination
Frustule, is then separated from water with flocculated method by it, is detected the nutrients such as the metal ion content and nitrogen phosphorus in water and is contained
Amount.The results show that treated, water quality is clarified, and metal ion content and nutritive element content are reduced to Group III water quality standard, is accorded with
Close emission request.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Sequence table
<110>Inst. of Hydrobiology, Chinese Academy of Sciences
<120>Chlorella W2 and the application of the heavy metal in high content of beary metal water body can be removed
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1831
<212> DNA
<213> Chlorella sp. W2
<400> 1
tagcgttacg acggcagtgc cagcttgcat gcctgcaggt cgacgatttg ttacgacttc 60
accttcctct aggtgggagg gtttaatgaa cttctcggcg gccgagagcg gagaccgccc 120
ccggtcgcca atccgaacac ttcaccagca cacccaatcg gtaggagcga cgggcggtgt 180
gtacaaaggg cagggacgta atcaacgcaa gctgatgact tgcgcttact aggcattcct 240
cgttgaagat taataattgc aataatctat ccccatcacg atgcagtttc aaagattacc 300
cgggcctctc ggccaaggct aggctcgttg aatgcatcag tgtagcgcgc gtgcggccca 360
gaacatctaa gggcatcaca gacctgttat tgcctcatgc ttccattggc tagtcgccaa 420
tagtccctct aagaagtccg ccggctggcg agccaaccgt gactatttag caggctgagg 480
tctcgttcgt taccggaatc aacctgacaa ggcaacccac caactaagaa cggccatgca 540
ccaccaccca tagaatcaag aaagagctct caatctgtca atcctcacta tgtctggacc 600
tggtaagttt tcccgtgttg agtcaaatta agccgcaggc tccacgcctg gtggtgccct 660
tccgtcaatt cctttaagtt tcagccttgc gaccatactc cccccggaac ccaaaaactt 720
tgatttctca taaggtgccg gcggagtcat cgaagaaaca tccgccgatc cctagtcggc 780
atcgtttatg gttgagacta ggacggtatc taatcgtctt cgagccccca actttcgttc 840
ttgattaatg aaaacatcct tggcaaatgc tttcgcagta gttcgtcttt cataaatcca 900
agaatttcac ctctgacaat gaaatacgaa tgcccccgac tgtccctctt aatcattact 960
ccggtcctac agaccaacag gataggccag agtcctatcg tgttattcca tgctaatgta 1020
ttcagagcgt aggcctgctt tgaacactct aatttactca aagtaacagc gccgactccg 1080
agtcccggac agtgaagccc aggagcccgt ccccggcaac aaggtgggcc ctgccagtgc 1140
acaccgaaac ggcggaccgg caggccccac ccgaaatcca actacgagct ttttaactgc 1200
agcaacttaa atatacgcta ttggagctgg aattaccgcg gctgctggca ccagacttgc 1260
cctccaattg atcctcgtta aggggtttag attgtactca ttccaattac cagacctgaa 1320
aaggcccagt attgttattt attgtcacta cctccctgtg ccaggattgg gtaatttgcg 1380
cgcctgctgc cttccttgga tgtggtagcc gtttctcagg ctccctctcc ggaatcgaac 1440
cctaatcctc cgtcacccgt taccaccatg gtaggcctct atcctaccat cgaaagttga 1500
tagggcagaa atttgaatga aacatcgccg gcgcaaggcc atgcgattcg tgaagttatc 1560
atgattcacc gcgagtcggg cagagcccgg tcggcctttt atctaataaa tacgtccctt 1620
ccagaagtcg ggatttacgc acgtattagc tctagattta ctacgggtat ccgagtagta 1680
ggtaccatca aataaactat aactgattta atgagccatt cgcagtttca cagtataaag 1740
cagtttatac ttagacatgc atggcttaat ctttgagaat ctctagagga tccccgggta 1800
ccgagctcga atcgtaatca tgtcaatgtt c 1831
Claims (7)
1. a kind of chlorella that can remove the heavy metal in high content of beary metal water body, the chlorella was on November 27th, 2017
It is preserved in China typical culture collection center, preserving number is CCTCC NO:M2017740.
2. application of the chlorella described in claim 1 in the heavy metal in removing high content of beary metal water body.
3. application according to claim 2, which is characterized in that the heavy metal is one in Cu, Zn, Ni, Co, Cr, Pb
Kind or multiple combinations.
4. a kind of method of the high content of beary metal sewage of processing, which is characterized in that including at chlorella described in claim 1
Manage the step in the sewage.
5. according to the method described in claim 4, it is characterized by comprising the following steps：
S1：The sewage is filtered, large particulate matter is removed；
S2：It is inoculated with the chlorella into filtered sewage, obtains chlorella-sewage culture；
S3：The chlorella-sewage culture is subjected to blowing air illumination cultivation；
S3：After culture 8-15 days, the chlorella in the chlorella-sewage culture is removed.
6. according to the method described in claim 5, it is characterized in that, the inoculum density of chlorella is OD in S2700Value is 0.1-
0.5。
7. method according to claim 5 or 6, which is characterized in that the condition cultivated described in S3 is 30 DEG C, 80 ± 5 μ
mol m-2s-1Intensity of illumination.
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| CN201810468898.3A CN108546649B (en) | 2018-05-16 | 2018-05-16 | Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109626582A (en) * | 2018-12-25 | 2019-04-16 | 武汉理工大学 | The method for removing lead in water removal using the modified microalgae of high phosphorus culture |
| CN114260307A (en) * | 2021-12-21 | 2022-04-01 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN114394670A (en) * | 2021-12-15 | 2022-04-26 | 海南大学 | Chlorella and application thereof in heavy metal wastewater treatment |
| CN114772742A (en) * | 2022-05-19 | 2022-07-22 | 武汉轻工大学 | Method for improving selenium-containing wastewater treatment capacity of chlorella |
| CN116836808A (en) * | 2023-08-24 | 2023-10-03 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
-
2018
- 2018-05-16 CN CN201810468898.3A patent/CN108546649B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109626582A (en) * | 2018-12-25 | 2019-04-16 | 武汉理工大学 | The method for removing lead in water removal using the modified microalgae of high phosphorus culture |
| CN114394670A (en) * | 2021-12-15 | 2022-04-26 | 海南大学 | Chlorella and application thereof in heavy metal wastewater treatment |
| CN114394670B (en) * | 2021-12-15 | 2023-05-23 | 海南大学 | Chlorella and application thereof in heavy metal wastewater treatment |
| CN114260307A (en) * | 2021-12-21 | 2022-04-01 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN114260307B (en) * | 2021-12-21 | 2022-08-19 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN114772742A (en) * | 2022-05-19 | 2022-07-22 | 武汉轻工大学 | Method for improving selenium-containing wastewater treatment capacity of chlorella |
| CN116836808A (en) * | 2023-08-24 | 2023-10-03 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
| CN116836808B (en) * | 2023-08-24 | 2023-12-12 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
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