CN108546650A - Chlorella W6 and the application of high content of beary metal Heavy Metals in Waters can quickly be removed - Google Patents
Chlorella W6 and the application of high content of beary metal Heavy Metals in Waters can quickly be removed Download PDFInfo
- Publication number
- CN108546650A CN108546650A CN201810469459.4A CN201810469459A CN108546650A CN 108546650 A CN108546650 A CN 108546650A CN 201810469459 A CN201810469459 A CN 201810469459A CN 108546650 A CN108546650 A CN 108546650A
- Authority
- CN
- China
- Prior art keywords
- chlorella
- high content
- sewage
- beary metal
- heavy metal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
- C12N1/125—Unicellular algae isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of chlorella that can quickly remove high content of beary metal Heavy Metals in Waters, the chlorella is preserved in China typical culture collection center on November 27th, 2017, and preserving number is CCTCC NO:M2017742.The chlorella W6 of the present invention has very high adsorption capacity to the heavy metal ion in water, there is very high removal rate to Cu, Zn, Ni, Co, Cr, Pb, Cd, far above the microalgaes for removing removing heavy metals most of at present, it is therefore particularly suitable for the processing higher polluted-water of content of beary metal.In addition to this, while removing removing heavy metals, chlorella W6 of the invention can also remove the nutriments such as N, P in water body, prevent the danger of body eutrophication.
Description
Technical field
The present invention relates to microdisk electrodes and sewage treatment field, more specifically it relates to which a kind of can remove high content of beary metal
The chlorella of heavy metal in water body and its application.
Background technology
The heavy metal amount of the mankind to environmental emission is increasing, not only seriously polluted soil and water body environment, also gives people
The health of class itself causes great harm.To heavy metal pollution water body treating, traditional method mainly has physics, chemistry and life
Object space method, including the precipitation method, chelating resin method, polymer chelating agent method, natural zeolite absorption method, membrane technology, activated carbon adsorption
Technique, ion-exchange etc..These method clean-up effects are relatively good, but technological process is complicated, cumbersome, of high cost, and
And be not suitable for large-scale application.
Microalgae is the microorganism of generally existing in various water bodys, and breeding is fast, adaptable, need to be only inoculated in water body few
The microalgae mother liquor of amount, after breeding a period of time in suitable environment, the amount of microalgae is with regard to dense needed for attainable treated sewage
Degree.Contain abundant nitrogen and phosphorus in sewage, good inorganic nutrients are provided for micro-algae propagation.In addition, microalgae can utilize it is photosynthetic
It acts on to obtain carbon source and energy, ensures the growth of microalgae without being additionally provided carbon source, reduce cost.Therefore, use is micro-
Algae treated sewage is a kind of not only good but also economical and practical sewage treatmentmethod of effect.
Studies have shown that some microalgaes have fixed function, process to be broadly divided into physical absorption and chemistry heavy metal ion
Absorption.By microalgae be applied to sewage in heavy metal fix, while can also reduce the nutrition contents such as the nitrogen phosphorus in water body with
And other harmful substances in absorption water body, it is that one kind is achieved many things at one stroke, economical and effective sewage water treatment method.However, very
Although more microalgaes have certain heavy metal metal ion adsorption capacity, but current microalgae is to the adsorption efficiency of heavy metal ion
It is limited, also, many microalgaes are also bad to the tolerance of heavy metal ion, and which greatly limits microalgae answering in sewage disposal
With.
It being resistant to high content of beary metal and the microalgae of energy efficient absorption heavy metal ion therefore, it is necessary to a kind of.
Invention content
Inventor is from field acquisition algae sample, through culture of isolated, obtain some heavy metals that one plant can remove in environment from
The algae strain of son.Its cell has core at round or ellipse, green, atrichia.Its 18S rDNA sequence such as SEQ is obtained through sequencing
ID NO:Shown in 1, combining form is it is found that it belongs to Chlorophyta Chlorella, therefore is named as chlorella W6, Classification system
Chlorella sp.W6。
It is found based on above, the present invention provides a kind of beads that can remove the heavy metal in high content of beary metal water body
Algae, the chlorella are preserved in China typical culture collection center on November 27th, 2017, and preserving number is CCTCC NO:
M2017742。
The present invention also provides application of the above-mentioned chlorella in the heavy metal in removing high content of beary metal water body.
In one embodiment, the heavy metal is one or more combinations in Cu, Zn, Ni, Co, Cr, Pb, Cd.
The present invention also provides a kind of methods of the high content of beary metal sewage of processing comprising handles institute with above-mentioned chlorella
State the step in sewage.
In one embodiment, it the described method comprises the following steps：
S1：The sewage is filtered, large particulate matter is removed；
S2：The chlorella is cultivated to logarithmic phase, chlorella culture is obtained；
S3：Chlorella cells are collected from the chlorella culture；
S3：The chlorella cells are forwarded in the sewage crossed through S1 step process, system for handling, room temperature are obtained
Lower processing 2-24 hours；
S4：Chlorella cells are removed from the system for handling.
In a preferred embodiment, the inoculum density of chlorella is OD in S2700Value is 1-2.
In a preferred embodiment, the method that chlorella cells are removed in S4 be it is a kind of in filtering, centrifugation, flocculation or
A variety of combinations.
The present invention chlorella W6 to the heavy metal ion in water have very high adsorption capacity, to Cu, Zn, Ni, Co, Cr,
Pb, Cd have very high removal rate, far above the microalgaes for removing removing heavy metals most of at present, are therefore particularly suitable for handling
The higher polluted-water of content of beary metal.In addition to this, while removing removing heavy metals, chlorella W6 of the invention can also be removed
The nutriments such as N, P in water body prevent the danger of body eutrophication.
Microbial preservation
The water sample of the microalgae being related to from field acquisition of the present invention is isolated, reflects through 18S rDNA sequencings and morphology
Fixed, which belongs to Chlorophyta Chlorella.The microalgae has been preserved on November 27th, 2017 to be sent to Wuhan City, Hubei Province force
The China typical culture collection center (CCTCC) of No. 299 Wuhan Universitys of prosperous area's Bayi Road in the school, preserving number are CCTCC NO:
M2017742, is named as chlorella W6, and Classification system is Chlorella sp.W6.
Description of the drawings
Fig. 1 is the light micrograph of the chlorella W6 of the present invention.
Specific implementation mode
Principles and features of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
1. the acquisition of chlorella W6
The chlorella W6 (Chlorella sp.W6) of the present invention is isolated by the algae-containing water sample from field acquisition,
Cellular morphology is as shown in Figure 1, cell is at round or ellipse, and green, atrichia has core.It, should through 18S rDNA combining forms
Microalgae belongs to Chlorophyta Chlorella.The microalgae is preserved in Wuhan University of Wuhan City of Hubei China province on November 27th, 2017
China typical culture collection center (CCTCC), preserving number be CCTCC NO:M2017742 is named as chlorella W6, Latin
Scientific name Chlorella sp.W6.
2. chlorella W6 is to the tolerance of heavy metal
After chlorella W6 is activated in small triangular flask, it is inoculated in the 500ml conical flasks of the BG11 culture mediums equipped with 300ml
In, initial OD700It is 0.1, condition of culture is 30 DEG C, 80 ± 5 μm of ol m-2s-1It ventilates and cultivates under illumination.
BG11 culture mediums are prepared by the following method：Take Stock II, Stock III, Stock IV, Stock
VI, Stock VII, each 1ml of Stock VIII, add 1.5g NaNO3, water is added to be settled to 1000ml.Each liquid storage ingredient such as 1 institute of table
Show, the ingredient of A5 is as shown in table 2.
The ingredient of each liquid storage during 1 BG11 culture mediums of table are prepared
2 A5 ingredients of table
3. chlorella W6 is to the removal efficiency of heavy metal in culture medium
After chlorella W6 grows into stationary phase, frustule is collected by centrifugation in 6000g, and frustule supreme heavy metal of transferring is contained
In the sewage of amount, the frustule of filtering removal after a certain period of time is used in combination ICP-MS to detect heavy metal ion remaining in culture solution.Knot
Fruit is as shown in table 3, and chlorella W6, which is handled 2-24 hours, has Cu, Zn, Ni, Co, Cr, Pb, Cd very high removal rate, handles
Time is short, and removal rate is therefore particularly suitable for processing heavy metal and contains far above the microalgaes for removing removing heavy metals most of at present
Measure higher polluted-water.
4 chlorella W6 of table is to heavy metal in culture medium
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Sequence table
<110>Inst. of Hydrobiology, Chinese Academy of Sciences
<120>Chlorella W6 and the application of high content of beary metal Heavy Metals in Waters can quickly be removed
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1838
<212> DNA
<213> Chlorella sp. W6
<400> 1
agctcggtac cggggatcct ctagagattt gatccttctg caggttcacc tacggaaacc 60
ttgttacgac ttctccttcc tctaggtggg agggtttaat gaacttctcg gcggccgaga 120
gcggagaccg cccccggtcg ccaatccgaa cacttcacca gcacacccaa tcggtaggag 180
cgacgggcgg tgtgtacaaa gggcagggac gtaatcaacg caagctgatg acttgcgctt 240
actaggcatt cctcgttgaa gattaataat tgcaataatc tatccccatc acgatgcagt 300
ttcaaagatt acccgggcct ctcggccaag gctaggctcg ttgaatgcat cagtgtagcg 360
cgcgtgcggc ccagaacatc taagggcatc acagacctgt tattgcctca tgcttccatt 420
ggctagtcgc caatagtccc tctaagaagt ccgccggctg gcgagccaac cgtgactatt 480
tagcaggctg aggtctcgtt cgttaccgga atcaacctga caaggcaacc caccaactaa 540
gaacggccat gcaccaccac ccatagaatc aagaaagagc tctcaatctg tcaatcctca 600
ctatgtctgg acctggtaag ttttcccgtg ttgagtcaaa ttaagccgca ggctccacgc 660
ctggtggtgc ccttccgtca attcctttaa gtttcagcct tgcgaccata ctccccccgg 720
aacccaaaaa ctttgatttc tcataaggtg ccggcggagt catcgaagaa acatccgccg 780
atccctagtc ggcatcgttt atggttgaga ctaggacggt atctaatcgt cttcgagccc 840
ccaactttcg ttcttgatta atgaaaacat ccttggcaaa tgctttcgca gtatttcgtc 900
tttcataaat ccaagaattt cacctctgac aatgaaatac gaatgccccc gactgtccct 960
cttaatcatt actccggtcc tacagaccaa caggataggc cagagtccta tcgtgttatt 1020
ccatgctaat gtattcagag cgtaggcctg ctttgaacac tctaatttac tcaaagtaac 1080
agcgccgact ccgagtcccg gacagtgaag cccaggagcc cgtccccggc aacaaggtgg 1140
gccctgccag tgcacaccga aacggcggac cggcaggccc cacccgaaat ccaactacga 1200
gctttttaac tgcagcaact taaatatacg ctattggagc tggaattacc gcggctgctg 1260
gcaccagact tgccctccaa ttgatcctcg ttaaggggtt tagattgtac tcattccaat 1320
taccagacct gaaaaggccc agtattgtta tttattgtca ctacctccct gtgtcaggat 1380
tgggtaattt gcgcgcctgc tgccttcctt ggatgtggta gccgtttctc aggctccctc 1440
tccggaatcg aaccctaatc ctccgtcacc cgttaccacc atggtaggcc tctatcctac 1500
catcgaaagt tgatagggca gaaatttgaa tgaaacatcg ccggcgcaag gccatgcgat 1560
tcgtgaagtt atcatgattc accgcgagtc gggcagagcc cggtcggcct tttatctaat 1620
aaatacgtcc cttccagaag tcgggattta cgcacgtatt agctctagat ttactacggg 1680
tatccgagta gtaggtacca tcaaataaac tataactgat ttaatgagcc attcgcagtt 1740
tcacagtata aagcagttta tacttagaca tgcatggctt aatctttgag acaagcatat 1800
gactactggc aggatcaacc aaatcgtcga catagtcg 1838
Claims (7)
1. a kind of chlorella that can quickly remove the heavy metal in high content of beary metal water body, the chlorella is in November, 2017
It is preserved within 27th China typical culture collection center, preserving number is CCTCC NO:M2017742.
2. application of the chlorella described in claim 1 in the heavy metal in removing high content of beary metal water body.
3. application according to claim 2, which is characterized in that the heavy metal is in Cu, Zn, N i, Co, Cr, Pb, Cd
One or more combinations.
4. a kind of method of the high content of beary metal sewage of processing, which is characterized in that including at chlorella described in claim 1
Manage the step in the sewage.
5. according to the method described in claim 4, it is characterized by comprising the following steps：
S1：The sewage is filtered, large particulate matter is removed；
S2：The chlorella is cultivated to logarithmic phase, chlorella culture is obtained；
S3：Chlorella cells are collected from the chlorella culture；
S3：The chlorella cells are forwarded in the sewage crossed through S1 step process, system for handling is obtained, locates at room temperature
Reason 2-24 hours；
S4：Chlorella cells are removed from the system for handling.
6. according to the method described in claim 5, it is characterized in that, the inoculum density of chlorella is OD in S3700Value is 1-2.
7. method according to claim 5 or 6, which is characterized in that in S4 remove chlorella cells method be filtering, from
One or more combination in the heart, flocculation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810469459.4A CN108546650B (en) | 2018-05-16 | 2018-05-16 | Chlorella W6 capable of quickly removing heavy metals in water with high heavy metal content and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810469459.4A CN108546650B (en) | 2018-05-16 | 2018-05-16 | Chlorella W6 capable of quickly removing heavy metals in water with high heavy metal content and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108546650A true CN108546650A (en) | 2018-09-18 |
| CN108546650B CN108546650B (en) | 2020-04-17 |
Family
ID=63495090
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810469459.4A Expired - Fee Related CN108546650B (en) | 2018-05-16 | 2018-05-16 | Chlorella W6 capable of quickly removing heavy metals in water with high heavy metal content and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108546650B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114260307A (en) * | 2021-12-21 | 2022-04-01 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN116836808A (en) * | 2023-08-24 | 2023-10-03 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
-
2018
- 2018-05-16 CN CN201810469459.4A patent/CN108546650B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114260307A (en) * | 2021-12-21 | 2022-04-01 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN114260307B (en) * | 2021-12-21 | 2022-08-19 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN116836808A (en) * | 2023-08-24 | 2023-10-03 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
| CN116836808B (en) * | 2023-08-24 | 2023-12-12 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108546650B (en) | 2020-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108587915A (en) | Chlorella W5 and the application of the heavy metal in high content of beary metal water body can be removed | |
| CN108546649A (en) | Chlorella W2 and the application of the heavy metal in high content of beary metal water body can be removed | |
| KR101834334B1 (en) | The Water Quality Control Facilities | |
| CN109576159A (en) | A kind of energy removes chlorella W4 and its application of the heavy metal in high content of beary metal water body | |
| CN104745511B (en) | Composite bacteria agent for repairing heavy oil pollution coastline and preparation method and application | |
| CN110241049B (en) | Pseudoalteromonas with algae dissolving capacity and application thereof to Karenia mikimotoi red tide | |
| CN212770335U (en) | Ecological management system of tail water breeds | |
| CN103497913A (en) | Method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment | |
| CN109576160A (en) | A kind of energy removes chlorella W3 and its application of the heavy metal in high content of beary metal water body | |
| CN108546650A (en) | Chlorella W6 and the application of high content of beary metal Heavy Metals in Waters can quickly be removed | |
| Bhuyar et al. | Salinity reduction from poly-chem-industrial wastewater by using microalgae (Chlorella sp.) collected from coastal region of peninsular Malaysia | |
| CN109576161A (en) | A kind of energy removes chlorella W1 and its application of the heavy metal in high content of beary metal water body | |
| CN102127517B (en) | Strain with heavy metal tolerance and applications thereof | |
| CN107285482A (en) | A kind of environmental protection ferment of purifying eutrophic water quality and preparation method thereof | |
| CN109022303B (en) | Composition with functions of dissolving algae and destroying microalgae air bags and application thereof | |
| CN116515665A (en) | Pseudomonas capable of degrading microcystin, immobilized microbial agent and application | |
| CN114874916B (en) | Fuscoporia fungus (Sarocladium kiliense) ZJ-1 and application thereof | |
| CN108130294A (en) | One kind is used for heavy metal pollution in-situ immobilization microorganism and application | |
| Sudha et al. | Microalgal efficiency for wastewater treatment | |
| CN113755336B (en) | Chlorella strain resistant to pollution and application thereof in livestock and poultry waste treatment | |
| RU2774294C1 (en) | Plankton eurybiontic strain of microalgae chlorella sorokiniana agt intended to improve the ecological state of water bodies | |
| CN103602619A (en) | Thauera sp. capable of degrading triethylamine as well breeding method and application of Thauera sp. | |
| CN220449973U (en) | High-level pond culture tail water treatment system | |
| CN117125839B (en) | Method for removing microplastic in sewage by utilizing microalgae-fungus symbiont | |
| CN111847607B (en) | Method for removing floating plants and phytoplankton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200417 |