CN109576159A - A kind of energy removes chlorella W4 and its application of the heavy metal in high content of beary metal water body - Google Patents
A kind of energy removes chlorella W4 and its application of the heavy metal in high content of beary metal water body Download PDFInfo
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- CN109576159A CN109576159A CN201710907107.8A CN201710907107A CN109576159A CN 109576159 A CN109576159 A CN 109576159A CN 201710907107 A CN201710907107 A CN 201710907107A CN 109576159 A CN109576159 A CN 109576159A
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- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims abstract description 50
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 229910052751 metal Inorganic materials 0.000 title claims abstract description 17
- 239000002184 metal Substances 0.000 title claims abstract description 17
- 229910052804 chromium Inorganic materials 0.000 claims abstract description 5
- 229910052759 nickel Inorganic materials 0.000 claims abstract description 5
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 5
- 239000010865 sewage Substances 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 20
- 238000005286 illumination Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000013618 particulate matter Substances 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 3
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 3
- 238000012851 eutrophication Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 17
- 241000195493 Cryptophyta Species 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 7
- 241000195654 Chlorella sorokiniana Species 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 108020004463 18S ribosomal RNA Proteins 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 3
- 201000004384 Alopecia Diseases 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
- C12N1/125—Unicellular algae isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of chlorella that can remove the heavy metal in high content of beary metal water body, the chlorella was preserved in China typical culture collection center on 2 22nd, 2017, and deposit number is CCTCC NO:M2017059.Chlorella W4 of the invention has the tolerance of height to the heavy metal ion in culture water body, when being grown in the water body containing high content of beary metal, to Zn, Ni, Cr has very high removal rate, and 98% or more is up to the removal rate of Zn, much higher than the removal efficiency of the microalgae Zn for removing removing heavy metals most of at present, is therefore particularly suitable for the processing higher polluted-water of Zn content.In addition to this, while removing removing heavy metals, chlorella W4 of the invention can also remove the nutriments such as N, P in water body, prevent the danger of water eutrophication.
Description
Technical field
The present invention relates to microdisk electrodes and sewage treatment field, more specifically it relates to which a kind of can remove high content of beary metal
The chlorella of heavy metal in water body and its application.
Background technique
The heavy metal amount of the mankind to environmental emission is increasing, not only seriously polluted soil and water body environment, also gives people
The health of class itself causes great harm.To heavy metal pollution water body treating, traditional method mainly has physics, chemistry and life
Object space method, including the precipitation method, chelating resin method, polymer chelating agent method, natural zeolite absorption method, membrane technology, activated carbon adsorption
Technique, ion-exchange etc..These method clean-up effects are relatively good, but process flow is complicated, cumbersome, at high cost, and
And be not suitable for large-scale application.
Microalgae is microorganism generally existing in various water bodys, and breeding is fast, adaptable, need to be only inoculated in water body few
The microalgae mother liquor of amount, after breeding a period of time in suitable environment, the amount of microalgae is with regard to dense needed for attainable processing sewage
Degree.Nitrogen and phosphorus rich in sewage, provide good inorganic nutrients for micro-algae propagation.In addition, microalgae can utilize it is photosynthetic
Effect guarantees the growth of microalgae without being additionally provided carbon source, reduces cost to obtain carbon source and energy.Therefore, use is micro-
Algae processing sewage is a kind of not only good but also economical and practical sewage treatmentmethod of effect.
Studies have shown that some microalgaes have fixed function to heavy metal ion, process is broadly divided into physical absorption and chemistry
Absorption.By microalgae be applied to sewage in heavy metal fix, while can also reduce the nutrition contents such as the nitrogen phosphorus in water body with
And other harmful substances in absorption water body, it is that one kind is achieved many things at one stroke, economical and effective sewage water treatment method.However, very
Although more microalgaes have certain heavy metal ion adsorbed ability, but current microalgae has the adsorption efficiency of heavy metal ion
Limit, also, many microalgaes are also bad to the tolerance of heavy metal ion, and which greatly limits microalgae answering in sewage treatment
With.
High content of beary metal is resistant to and the microalgae of energy efficient absorption heavy metal ion therefore, it is necessary to a kind of.
Summary of the invention
Inventor is from field acquisition algae sample, through culture of isolated, obtain some heavy metals that one plant can remove in environment from
The algae strain of son.Its cell has core at round or ellipse, green, atrichia.Its 18S rDNA sequence such as SEQ is obtained through sequencing
Shown in ID NO:1, which is compared in ncbi database, finds the Chlorella of itself and Chlorophyta Chlorella
The sorokiniana degree of approximation is relatively high, therefore is named as chlorella W4.
Based on the above discovery, the present invention provides a kind of beads that can remove the heavy metal in high content of beary metal water body
Algae, the chlorella were preserved in China typical culture collection center on 2 22nd, 2017, and deposit number is CCTCC NO:
M2017059。
The present invention also provides above-mentioned chlorellas to remove the application in the heavy metal in high content of beary metal water body.
In one embodiment, the heavy metal is one of Zn, Ni, Cr or multiple combinations.
In a preferred embodiment, the heavy metal is Zn.
The present invention also provides a kind of methods for handling high content of beary metal sewage comprising handles institute with above-mentioned chlorella
State the step in sewage.
In one embodiment, it the described method comprises the following steps:
S1: the sewage is filtered, and removes large particulate matter；
S2: being inoculated with the chlorella into filtered sewage, obtains chlorella-sewage culture；
S3: the chlorella-sewage culture is subjected to blowing air illumination cultivation；
S3: after culture 8-15 days, the chlorella in the chlorella-sewage culture is removed.
In a preferred embodiment, the inoculum density of chlorella is OD in S2700Value is 0.1-0.5.
In a preferred embodiment, the condition of culture described in S3 is 30 DEG C, 80 ± 5 μm of ol m-2s-1Illumination is strong
Degree.
Chlorella W4 of the invention has high tolerance to the heavy metal ion in culture water body, is containing a high huge sum of money
When growing in the water body of category content, there is very high removal rate to Zn, Ni, Cr, and 98% or so is up to the removal rate of Zn,
Much higher than the removal efficiency of the microalgae Zn for removing removing heavy metals most of at present, it is higher to be therefore particularly suitable for processing Zn content
Polluted-water.In addition to this, while removing removing heavy metals, chlorella W4 of the invention can also remove N, P etc. in water body
Nutriment prevents the danger of water eutrophication.Algae strain can be used for handling trade effluent, rural sewage and other Gao Chong
The water body of tenor.
Microbial preservation
The water sample of the microalgae being related to from field acquisition of the present invention is isolated, reflects through 18S rDNA sequencing and morphology
Fixed, which belongs to Chlorophyta Chlorella, and 18S rDNA sequence is compared with the Chlorella sorokiniana degree of approximation
It is high.The microalgae has been preserved in the China typical culture collection of Wuhan University, Wuhan City, Hubei China province for 2 months on the 22nd in 2017
Center (CCTCC), deposit number are CCTCC NO:M2017059, are named as chlorella W4, Classification system Chlorella
sorokiniana W4。
Detailed description of the invention
Fig. 1 is the light micrograph of chlorella W4 of the invention；
Fig. 2 is the life that chlorella W4 is cultivated in the BG11 culture medium in BG11 culture medium and added with heavy metal respectively
Long curve；
Fig. 3 is that control algae chlorella G32 is trained in the BG11 culture medium in BG11 culture medium and added with heavy metal respectively
Support obtained growth curve.
Specific embodiment
Principles and features of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the invention.
1. the acquisition of chlorella W4
Chlorella W4 (Chlorella sorokiniana W4) of the invention from the algae-containing water sample of field acquisition by dividing
From obtaining, for cellular morphology as shown in Figure 1, cell is at round or ellipse, green, atrichia has core.It is sequenced through 18S rDNA
Identification, the microalgae belong to Chlorophyta Chlorella.The microalgae has been preserved in Wuhan City, Hubei China province force on 2 22nd, 2017
The China typical culture collection center (CCTCC) of Chinese university, deposit number are CCTCC NO:M2017059, and Classification system is
Chlorella sorokiniana W4 is named as chlorella W4, Classification system Chlorella sorokiniana W4.
2. chlorella W4 is to the tolerance of heavy metal
After chlorella W4 is activated in small triangular flask, it is inoculated in the BG11 culture equipped with 300ml containing high content of beary metal
In the 500ml conical flask of base, initial OD700It is 0.1, condition of culture is 30 DEG C, 80 ± 5 μm of ol m-2s-1It ventilates and cultivates under illumination.
BG11 culture medium containing high content of beary metal is prepared by the way that heavy metal mother liquor is added in BG11 culture medium.Its
In, BG11 culture medium is prepared by the following method: take Stock II, Stock II I, Stock IV, Stock VI,
Each 1ml of Stock VII, Stock VIII, adds 1.5g NaNO3, water is added to be settled to 1000ml.Each liquid storage ingredient is as shown in table 1,
The ingredient of A5 is as shown in table 2.
The ingredient of each liquid storage in the preparation of table 1BG11 culture medium
Table 2A5 ingredient
The addition of heavy metal is according to V class (heavily contaminated) water quality mark in " water environment quality standard " (GB3838-2002)
Quasi- and " urban wastewater treatment firm pollutant emission standard " is arranged.Heavy metal mother liquor is arranged to 2000 ×.Table 3 is heavy metal
Mother liquid concentration and working concentration.
3 heavy metal mother liquid concentration of table and working concentration
After inoculation, the OD of chlorella culture is detected daily700Value, is fabricated to growth curve, with BG11 culture medium culture
Chlorella be control, observation chlorella W4 is to the tolerance of heavy metal.
As a result as shown in Fig. 2, the growth curve cultivated in BG11 culture medium of chlorella W4 with added with heavy metal
The growth curve cultivated in BG11 culture medium is not much different early period, to the later period, is added to small in the BG11 culture medium of heavy metal
Ball algae W4 even grows more preferable, it is seen that chlorella W4 has high tolerance to heavy metal.Fig. 3 is that inventor was separating
Another chlorella G32 being separated in journey is cultivated in the BG11 culture medium in BG11 culture medium and added with heavy metal respectively
The growth curve arrived, the results show that its life as known most of green algas, in the BG11 culture medium added with heavy metal
Length is greatly inhibited.
3. chlorella W4 is to the removal efficiency of heavy metal in culture medium
After chlorella W4 grows into stationary phase, 6000g is collected by centrifugation frustule, and with residual in ICP-MS detection culture solution
Remaining heavy metal ion.The results are shown in Table 4, and chlorella W4 has very high removal rate to Zn, Ni, Cr, and goes to Zn
Except rate is up to 98% or so, much higher than most of at present for going the microalgae of removing heavy metals, it are therefore particularly suitable for processing Zn and contain
Measure higher polluted-water.4 chlorella W4 of table is to heavy metal in culture medium
4. the trade effluent application that chlorella W4 handles high content of beary metal
The trade effluent of high content of beary metal is filtered, the large particulate matter in sewage is removed.Then into sewage
It is inoculated with chlorella W4, inoculum density OD700To 0.1-0.5.At 30 DEG C, 80 ± 5 μm of ol m-2s-17-15 is cultivated under intensity of illumination
Frustule, is then separated from water with flocculated method by it, is detected the nutrients such as the metal ion content and nitrogen phosphorus in water and is contained
Amount.The results show that treated, water quality is clarified, and metal ion content and nutritive element content are reduced to Group III water quality standard, is accorded with
Close emission request.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Sequence table
<110>Wuhan Zao You Biotechnology Co., Ltd
<120>a kind of chlorella W4 that can remove the heavy metal in high content of beary metal water body and its application
<130> 1
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1831
<212> DNA
<213>chlorella W4 ()
<400> 1
atgggtacga cggtcagtgc cagcttgcat gcctgcaggt cgacgatttg ttacgacttc 60
accttcctct aggtgggagg gtttaatgaa cttctcggcg gccgagagcg gagaccgccc 120
ccggtcgcca atccgaacac ttcaccagca cacccaatcg gtaggagcga cgggcggtgt 180
gtacaaaggg cagggacgta atcaacgcaa gctgatgact tgcgcttact aggcattcct 240
cgttgaagat taataattgc aataatctat ccccatcacg atgcagtttc aaagattacc 300
cgggcctctc ggccaaggct aggctcgttg aatgcatcag tgtagcgcgc gtgcggccca 360
gaacatctaa gggcatcaca gacctgttat tgcctcatgc ttccattggc tagtcgccaa 420
tagtccctct aagaagtccg ccggctggcg aaccaaccgt gactatttag caggctgagg 480
tctcgttcgt taccggaatc aacctgacaa ggcaacccac caactaagaa cggccatgca 540
ccaccaccca tagaatcaag aaagagctct caatctgtca atcctcacta tgtctggacc 600
tggtaagttt tcccgtgttg agtcaaatta agccgcaggc tccacgcctg gtggtgccct 660
tccgtcaatt cctttaagtt tcagccttgc gaccatactc cccccggaac ccaaaaactt 720
tgatttctca taaggtgccg gcggagtcat cgaagaaaca tccgccgatc cctagtcggc 780
atcgtttatg gttgagacta ggacggtatc taatcgtctt cgagccccca actttcgttc 840
ttgattaatg aaaacatcct tggcaaatgc tttcgcagta gttcgtcttt cataaatcca 900
agaatttcac ctctgacaat gaaatacgaa tgcccccgac tgtccctctt aatcattact 960
ccggtcctac agaccaacag gataggccag agtcctatcg tgttattcca tgctaatgta 1020
ttcagagcgt aggcctgctt tgaacactct aatttactca aagtaacagc gccgactccg 1080
agtcccggac agtgaagccc aggagcccgt ccccggcaac aaggtgggcc ctgccagtgc 1140
acaccgaaac ggcggaccgg caggccccac ccgaaatcca actacgagct ttttaactgc 1200
agcaacttaa atatacgcta ttggagctgg aattaccgcg gctgctggca ccagacttgc 1260
cctccaattg atcctcgtta aggggtttag attgtactca ttccaattac cagacctgaa 1320
aaggcccagt attgttattt attgtcacta cctccctgtg tcaggattgg gtaatttgcg 1380
cgcctgctgc cttccttgga tgtggtagcc gtttctcagg ctccctctcc ggaatcgaac 1440
cctaatcctc cgtcacccgt taccaccatg gtaggcctct atcctaccat cgaaagttga 1500
tagggcagaa atttgaatga aacatcgccg gcgcaaggcc atgcgattcg tgaagttatc 1560
atgattcacc gcgagtcggg cagagcccgg tcggcctttt atctaataaa tacgtccctt 1620
ccagaagtcg ggatttacgc acgtattagc tctagattta ctacgggtat ccgagtagta 1680
ggtaccatca aataaactat aactgattta atgagccatt cgcagtttca cagtataaag 1740
cagtttatac ttagacatgc atggcttaat ctttgagaca atctctagag gatccccggg 1800
taccgagctc gaatcgtaat tcatgttcca a 1831
Claims (8)
1. a kind of chlorella that can remove the heavy metal in high content of beary metal water body, the chlorella was on 2 22nd, 2017
It is preserved in China typical culture collection center, deposit number is CCTCC NO:M2017059.
2. chlorella described in claim 1 is removing the application in the heavy metal in high content of beary metal water body.
3. application according to claim 2, which is characterized in that the heavy metal is one of Zn, Ni, Cr or a variety of groups
It closes.
4. application according to claim 3, which is characterized in that the heavy metal is Zn.
5. a kind of method for handling high content of beary metal sewage, which is characterized in that including at chlorella described in claim 1
Manage the step in the sewage.
6. according to the method described in claim 5, characterized by comprising the following steps:
S1: the sewage is filtered, and removes large particulate matter；
S2: being inoculated with the chlorella into filtered sewage, obtains chlorella-sewage culture；
S3: the chlorella-sewage culture is subjected to blowing air illumination cultivation；
S3: after culture 8-15 days, the chlorella in the chlorella-sewage culture is removed.
7. according to the method described in claim 6, it is characterized in that, the inoculum density of chlorella is OD in S2700Value is 0.1-
0.5。
8. method according to claim 6 or 7, which is characterized in that the condition of culture described in S3 is 30 DEG C, 80 ± 5 μ
mol m-2s-1Intensity of illumination.
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| CN201710907107.8A CN109576159B (en) | 2017-09-29 | 2017-09-29 | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof |
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| CN201710907107.8A CN109576159B (en) | 2017-09-29 | 2017-09-29 | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof |
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| CN109576159B CN109576159B (en) | 2022-07-26 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111621423A (en) * | 2020-05-27 | 2020-09-04 | 武汉理工大学 | High-phosphorus-loading living microalgae and preparation method and application thereof |
| CN114260307A (en) * | 2021-12-21 | 2022-04-01 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN115026128A (en) * | 2022-06-17 | 2022-09-09 | 中国科学院南京土壤研究所 | Rice field heavy metal inhibition control and absorption method based on periphyton |
| CN116836808A (en) * | 2023-08-24 | 2023-10-03 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111621423A (en) * | 2020-05-27 | 2020-09-04 | 武汉理工大学 | High-phosphorus-loading living microalgae and preparation method and application thereof |
| CN114260307A (en) * | 2021-12-21 | 2022-04-01 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN114260307B (en) * | 2021-12-21 | 2022-08-19 | 海南大学 | Application of tubulose algae and microbial agent and method for removing environmental heavy metals |
| CN115026128A (en) * | 2022-06-17 | 2022-09-09 | 中国科学院南京土壤研究所 | Rice field heavy metal inhibition control and absorption method based on periphyton |
| CN115026128B (en) * | 2022-06-17 | 2023-08-18 | 中国科学院南京土壤研究所 | Rice field heavy metal resistance control and digestion method based on periphyton |
| CN116836808A (en) * | 2023-08-24 | 2023-10-03 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
| CN116836808B (en) * | 2023-08-24 | 2023-12-12 | 广东省科学院生态环境与土壤研究所 | Acidophilic cadmium-resistant microalgae and application thereof in soil remediation |
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| CN109576159B (en) | 2022-07-26 |
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