CN108841748A - Sinorhizobium nitrogen-fixing bacteria strain H6 and its application - Google Patents
Sinorhizobium nitrogen-fixing bacteria strain H6 and its application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/40—Fabaceae, e.g. beans or peas
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of Sinorhizobium nitrogen-fixing bacteria strain H6 and its application, the Sinorhizobium nitrogen-fixing bacteria strain H6 is preserved in Wuhan University's China typical culture collection center May 7 in 2018, is referred to as CCTCC, and deposit number is CCTCC NO:M2018255.Nodule azotobacter strain H6 is the rhizobium captured in soil by alfalfa plants, rhizobium are separated and are purified with gained from fresh root nodule, and the 16S rDNA partial sequence of bacterial strain is measured, by Phylogenetic Analysis, it is determined as Sinorhizobium Pseudomonas（Sinorhizobium）New strains system, and be named as H6.Nodule azotobacter strain H6 of the present invention is proved by the test of sand culture tieback：It, which has, alleviates clover salt damage, the characteristic of high-efficiency nitrogen-fixing, and under the conditions of salt treatment, clover biomass can be increased by being inoculated with the rhizobium, improved nitrogen, phosphorus, potassium content in plant body, be applicable to the Saline Regions such as Xinjiang, one of the measure as clover conventional cultivation.
Description
Technical field
The present invention relates to a kind of Sinorhizobium nitrogen-fixing bacteria strain H6 (Sinorhizobium sp.) and its applications.
Background technique
There are some microorganisms the nitrogen in air can be reduced to ammonia in nature, provides nitrogen for microorganism and plant
Source, this phenomenon are referred to as biological nitrogen fixation.According to the relationship between higher plant and nitrogen-fixing microorganism, biological nitrogen fixation is divided into
Three itself fixed nitrogen, symbiotic nitrogen fixation, association nitrogen fixation systems.
Rhizobium are the nonspore-bearing agrobacteriums of a kind of Gram-negative, can be formed with leguminous plant clover mutually beneficial
Symbiosis.This symbiosis has the characteristics that amount of nitrogen fixation is big, resistance ability is strong.
Alfalfa is the high quality forage of domestic animal, extensive in grown worldwide area, there is the title of " cash crops ", raw in soil
There is indispensable critical role in state protection and agricultural production.Xinjiang is located in NW China border area, unique weather conditions
It is suitable for alfalfa growing, but the salination of In The Soil of Xinjiang seriously affects the raising of alfalfa output.Therefore, clover salt resistance ability is improved
It is significant for the alfalfa industry of Development in Xinjiang.It is many studies have shown that clover salt tolerant can be improved in Rhizobium Inoculation simultaneously
Property, in order to alleviate clover salt damage, it is necessary to which the rhizobium of breeding salt tolerant adapt to salinization soil, could obtain high yield in this way.
Summary of the invention
The object of the present invention is to provide a kind of Sinorhizobium nitrogen-fixing bacteria strain H6 and its application, the Sinorhizobiums
Nitrogen-fixing bacteria strain H6 is preserved in Wuhan University's China typical culture collection center May 7 in 2018, is referred to as CCTCC,
Deposit number is CCTCC NO:M2018255.Nodule azotobacter strain H6 is the root nodule captured in soil by alfalfa plants
Bacterium is separated and is purified to rhizobium gained from fresh root nodule, and surveyed to the 16S rDNA partial sequence of bacterial strain
It is fixed, by Phylogenetic Analysis, it is determined as the new strains system of Sinorhizobium Pseudomonas (Sinorhizobium), and be named as H6.
Nodule azotobacter strain H6 of the present invention is proved by the test of sand culture tieback：It, which has, alleviates clover salt damage, high-efficiency nitrogen-fixing
Characteristic, under the conditions of salt treatment, clover biomass can be increased by being inoculated with the rhizobium, improve nitrogen, phosphorus, potassium content in plant body,
The Saline Regions such as Xinjiang are applicable to, one of the measure as clover conventional cultivation.
A kind of Sinorhizobium nitrogen-fixing bacteria strain H6, Sinorhizobium H6Sinorhizobium of the present invention
Sp.H6 belongs to Sinorhizobium Pseudomonas, is preserved in Wuhan University's China typical culture collection center on May 7th, 2018
CCTCC, deposit number are CCTCC NO:M 2018255.
The Sinorhizobium nitrogen-fixing bacteria strain H6 is preparing legume symbiosis fixed nitrogen and is alleviating the application in salt damage.
Sinorhizobium nitrogen-fixing bacteria strain H6 of the present invention is preserved in the training of Wuhan University's Chinese Typical Representative May 7 in 2018
Object collection is supported, CCTCC is referred to as, deposit number is CCTCC NO:M 2018255, address：Wuhan, China, Wuhan are big
It learns, is separated in the alfalfa Phylloxera that three level ground experimental farm of Xinjiang Agricultural Univ is planted in July, 2016.
The characteristics of Sinorhizobium nitrogen-fixing bacteria strain H6 is：
One, the separation of Sinorhizobium nitrogen-fixing bacteria strain H6：
1) fresh root nodule is taken, is rinsed with water completely, and blot surface moisture with filter paper；
2) it is put into the alcohol that volumetric concentration is 95% and handles 3 minutes, take out, with aseptic water washing 5-6 times, place into body
Product concentration is 0.2%HgCl2Sterilizing 5 minutes is taken out, then with aseptic water washing 5-6 times；
3) fresh root nodule is cut in half again on the sterilized glass slide of flame, clamps half of tumor with aseptic nipper, cuts
Mouthful towards YMA medium surface cross, the formula of YMA medium is as shown in table 1, after inversion at 28 DEG C of temperature dark culturing；
1 YMA of table (Yeast Mannitol Agar) culture medium prescription (/L)
4) after growing thallus, rhizobium are tentatively judged whether it is according to two aspects of colonial morphology and thalli morphology：
Colonial morphology：The bacterium colony of rhizobium is that circle, milky, translucent, neat in edge, cement are more or less, is cultivated
3-5 days colony diameters are fast-growing Rhizobium up to 2-4mm, and 7-10 days bacterium colony ability 1mm of culture are Slow_growing rhizobia；
Thalli morphology：Label is tentatively confirmed as rhizobium bacterium colony, therefrom picking lawn smear, carries out Gram's staining, oil
Under the microscope, interior often to contain beta-hydroxy-butanoic acid, that is, it reflects the strong seemingly particle in cavity or keeps thallus in the form of link, Gram-negative
(G-), no gemma, thallus are single or pairs of；
5) according to two aspects of colonial morphology and thalli morphology, dominant colony is selected from culture medium with oese and is continued
It crosses and cultivates on new YMA medium, bacterium colony situation is observed after 3 days；
Two, the property of Sinorhizobium nitrogen-fixing bacteria strain H6：
Morphological feature：This bacterial strain is on colonial morphology, and circle is smaller, milky, translucent, cement is more, in YMA plate
Diameter after upper growth 3 days is 0.8-2.0mm；
Cultural character：The optimum growing condition of the bacterium is：28 DEG C of temperature, revolving speed 200r/min；Extensive carbon can be utilized
Source and nitrogen source；
Physiological property：It is G through Gram's staining reaction microscopy-, it is in small rod-short；
Functional characteristic：Rhizobium H6 has the characteristics that amount of nitrogen fixation is big, salt tolerance is strong, when thallus is released in natural environment
When, it is harmless to people, animal and plant, it does not pollute the environment, group's abundance of rhizobium in soil is increased instead, to pulse family
The nodulation and nitrogen fixation of plant, which alleviates salt damage, obvious effect；
Three, the 16S rDNA sequence of Sinorhizobium nitrogen-fixing bacteria strain H6：
In order to identify the Phylogenetic of rhizobium, separated bacterial strain 16S rDNA sequence is sequenced, first
The total DNA of bacterial strain is extracted, PCR specific amplification is then carried out, pcr amplification product is examined on 1.0% agarose gel electrophoresis
After testing, it to be used for sequencing, sequencing result such as SEQ ID NO:Shown in 1：
Rhizobium (Sinorhizobium)
>H6
Reference strains, 16S of the application software MEGA to isolated strains and reference strains are obtained from GenBank database
RDNA partial sequence is analyzed, and is constructed the Phylogenetic Relationships figure of isolated strains and reference culture, is obtained reflecting to be measured
The phylogenetic tree evolved between alfalfa Phylloxera bacterial strain and reference strains with development relationship, so that it is determined that the root nodule separated
Bacterium H6 is the new strains of Sinorhizobium Pseudomonas (Sinorhizobium).
Compared with prior art, the present invention has the advantages that：
Sinorhizobium nitrogen-fixing bacteria strain H6 of the present invention has salt tolerance characteristic, and being inoculated with the rhizobium can be improved lucerne
Mu biomass, can be used for actual production；
By being inoculated with Sinorhizobium nitrogen-fixing bacteria strain H6 of the invention, can reach improves clover nitrogen nutrition, alleviates salt damage, from
And achieve the purpose that clover high yield and fertilizing soil；
Sinorhizobium nitrogen-fixing bacteria strain H6 of the present invention is suitable for Xinjiang salinization soil area, as clover routine
One of measure of cultivation.
Detailed description of the invention
Fig. 1 is Sinorhizobium nitrogen-fixing bacteria strain H6 of the present invention separation and the colonial morphology on YMA medium；
Fig. 2 is observation image of the invention Sinorhizobium nitrogen-fixing bacteria strain H6 after Gram's staining under the microscope；
Fig. 3 is the 16S rDNA Analysis of Partial Phylogenetic dendrogram of Sinorhizobium nitrogen-fixing bacteria strain H6 of the present invention；
Fig. 4 is present invention inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 to biomass influence diagram, will be inoculated with reference culture
Lucerne three (ZM) and Algonquin (GJ) plant strain growth two in USDA1002 (1002), H6, and the alfalfa variety that is not inoculated with
Week the salt treatment nutrient solution (0 and 100mM NaCl) of various concentration NaCl is added, pours every three days once, and two weeks after treatment
It harvests, the data in figure are four duplicate average values of biology with standard error；
Fig. 5 is present invention inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 to plant above ground portion, root nitrogen content influence diagram, will be connect
It plants reference culture USDA1002 (1002), H6, and lucerne three (ZM) and Algonquin (GJ) plant in the alfalfa variety not being inoculated with
Strain growth two weeks, is added the salt treatment nutrient solution (0 and 100mM NaCl) of different NaCl concentrations, pours every three days once, and locating
It harvests within two weeks after reason, the data in figure are four duplicate average values of biology with standard error；
Fig. 6 is that present invention inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 influences plant above ground portion, root potassium content, will be inoculated with
Lucerne three (ZM) and Algonquin (GJ) plant in reference culture USDA1002 (1002), H6, and the alfalfa variety that is not inoculated with
Growth two weeks, is added the salt treatment nutrient solution (0 and 100mM NaCl) of different NaCl concentrations, pours every three days once, and handling
It harvests within two weeks afterwards, the data in figure are four duplicate average values of biology with standard error；
Fig. 7 is that present invention inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 influences plant above ground portion, root phosphorus content, will be inoculated with
Lucerne three (ZM) and Algonquin (GJ) plant in reference culture USDA1002 (1002), H6, and the alfalfa variety that is not inoculated with
Growth two weeks, is added the salt treatment nutrient solution (0 and 100mM NaCl) of different NaCl concentrations, pours every three days once, and handling
It harvests within two weeks afterwards, the data in figure are four duplicate average values of biology with standard error.
Specific embodiment
Embodiment 1
The separation and purifying of Sinorhizobium nitrogen-fixing bacteria strain H6：
Fresh root nodule is taken, is rinsed with water completely, blots surface moisture with filter paper, is first put into the ethyl alcohol that volumetric concentration is 95%
Middle processing 3 minutes, takes out, and with aseptic water washing 5-6 times, placing into volumetric concentration is 0.2%HgCl2Sterilizing 5 minutes is taken out,
With aseptic water washing 5-6 times, fresh root nodule is cut in half again on the sterilized glass slide of flame then, is pressed from both sides with aseptic nipper
Firmly half of tumor, notch are crossed towards YMA medium surface, and the formula of YMA medium is as shown in table 1, at 28 DEG C of temperature after inversion
Lower culture；
1 YMA of table (Yeast Mannitol Agar) culture medium prescription (/L)
After growing thallus, selected from plate in form as the bacterium colony of rhizobium is in flat lining out culture, after 3 days
Bacterium colony situation is observed, is observed always 15 days, because slow raw rhizobium need 7-15 days bacterium colony occur；According to colonial morphology and bacterium
Two aspects of volume morphing can tentatively judge whether it is rhizobium：
Colonial morphology：The bacterium colony of rhizobium is circle, milky, translucent, neat in edge, and cement is more or less, culture
3-5 days colony diameters are fast-growing Rhizobium up to 2-4mm, and 7-10 days bacterium colony ability 1mm of culture are Slow_growing rhizobia；
Thalli morphology：Label is tentatively confirmed as rhizobium bacterium colony, therefrom picking lawn smear, carries out Gram's staining, oil
Under the microscope, the dialister bacterium that rhizobium are (0.5-0.9) × (1.2-0.3) μm, it is interior often to contain beta-hydroxy-butanoic acid, that is, reflect strong
Like empty particle or keep thallus in the form of link, Gram-negative (G-), no gemma, thallus is single or pairs of, such as bacterium colony thallus
As rhizobium, then that bacterium colony marked access test tube slant culture is saved；
The bacterial strain of the present embodiment is fast-growing type, is that circle is smaller, milky, translucent, cement is more on colonial morphology；
Diameter after growing 3 days on YMA plate is 0.8-2.0mm (Fig. 1)；Optimum growing condition is：28 DEG C of temperature, revolving speed 200r/
Min can utilize extensive carbon source and nitrogen source；It is G through Gram's staining reaction microscopy-, it is in small rod-short (Fig. 2).
The method isolated and purified using this is purified repeatedly by several generations, the scribing line training in solid medium YMA (table 1)
It supports, obtains more plants of pure bacterium, pure bacterium passes through its nodulation and nitrogen fixation ability of tieback verification experimental verification, and the present embodiment isolates and purifies salt tolerant height
Imitate Sinorhizobium nitrogen-fixing bacteria strain H6.
Embodiment 2
The 16S rDNA sequence of Sinorhizobium nitrogen-fixing bacteria strain H6：
The total DNA of bacterial strain is extracted using kit, carries out PCR specific amplification, agar of the pcr amplification product 1.0%
After being examined in sugared gel electrophoresis, it is sent to Sheng Gong company and carries out sequencing, sequencing result such as SEQ ID NO:Shown in 1；
Rhizobium (Sinorhizobium)
>H6
Reference strains, 16S of the application software MEGA to isolated strains and reference strains are obtained from GenBank database
RDNA partial sequence is analyzed, and the systematic growth of building isolated strains H6 and reference culture USDA1002 (X67222.2) are closed
System's figure, as shown in figure 3, so that it is determined that the rhizobium H6 separated is the new bacterium of Sinorhizobium Pseudomonas (Sinorhizobium)
Strain.
Embodiment 3
Tieback test：
This experiment is middle lucerne No. three (ZM) and Algonquin (GJ) using alfalfa variety, is filtered out according to solution culture,
ZM is vegetative growth stage salt-enduring cultivars, and GJ is salt density value kind, as rhizobium salt tolerance clover tieback experimental plants material
Material；Setting does not meet rhizobium (- Ri), meets rhizobium reference culture USDA1002 and tri- kinds of H6 of Sinorhizobium nitrogen-fixing bacteria strain connect
Bacterium processing, salt treatment are divided into two NaCl salinity gradients of 0mM and 100mM, and randomized complete-block design, each place are taken in test
Manage 4 repetitions；
It tests and is carried out in 811 culturing room of Agricultural University Of South China's root system biology center, the quartz sand of wash clean is pressed
Carse, medium and small sand presses 1:2:1 high-temperature sterilization after mixing, is packed into diameter 5cm, and in the basin of high 15cm, alfalfa seed is by above-mentioned
Method sterilizing is placed on dark culture 12h in the wet filter paper culture dish of 1/4Hoagland nutrient solution, and take the exposing tip of a root has work
The seed of power moves into potting, every 4 seeds of basin point, thinning after a week, and carries out connecing bacterium processing, and bacterium solution requires OD value to be greater than
1, it takes around 1mL access clover root, connects bacterium and carry out salinity processing after a week, the NaCl processing of setting 0mM and 100mM claims
It takes the NaCl of respective concentration that 1/4Hoagland Poor nitrogen nutrition liquid is added, adjusts between pH 5.8-6.0,3 days primary；
Plant strain growth harvests after 4 weeks, measures indices, as a result as follows：By Fig. 4 Rhizobium Inoculation to clover biomass
It can be seen that, comparison does not connect the plant of bacterium in influence, in the case where salt treatment is not added, inoculation reference culture USDA1002 and Chinese root
Tumor nitrogen-fixing bacteria strain H6 can increase the biomass of clover, and under salt treatment, inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 increases
The effect of biomass is better than reference culture USDA1002, and is considerably better than and is not inoculated with processing (table 2)；
2 tieback of table experiment in salt treatment, genotype, inoculation processing and its reciprocation on alfalfa growing influence three because
Plain the results of analysis of variance
Note：1) F value is three-factor analysis of variance result.Wherein, S represents salt treatment, and G represents genotype, and I is represented at inoculation
Reason, S × G represent the reciprocation of salt treatment and genotype, and S × I represents the reciprocation of salt treatment and inoculation processing, G × I
The reciprocation of genotype and inoculation processing is represented, the interaction that S × G × I is represented between salt treatment, genotype and inoculation processing is made
With.2)*： P<0.05, * *：0.001<P<0.01, * * *：P<0.001, ns：Difference is not significant；
Influence by Fig. 5 inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 to clover overground part and root nitrogen content：Comparison does not connect
The plant of bacterium, in the case where salt treatment is not added, inoculation reference culture USDA1002 and Sinorhizobium nitrogen-fixing bacteria strain H6 can increase lucerne
The overground part and root nitrogen content of Mu, and effect is identical both in lucerne 3 in salt-enduring cultivars, and salt density value kind Ah
The effect that reference culture USDA1002 is inoculated in Er Gangjin is better than Sinorhizobium nitrogen-fixing bacteria strain H6.And under salt treatment, inoculation
The effect that Sinorhizobium nitrogen-fixing bacteria strain H6 increases root nitrogen content is better than reference culture USDA1002, and is considerably better than and does not connect
Kind processing (table 2)；
Influence by Fig. 6 inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 to clover overground part and root potassium content：Comparison does not connect
The plant of bacterium, in the case where salt treatment is not added, inoculation reference culture USDA1002 and Sinorhizobium nitrogen-fixing bacteria strain H6 can increase lucerne
The overground part potassium content of Mu, and effect is identical both in lucerne 3 in salt-enduring cultivars, and in salt density value kind Algonquin
The effect of middle inoculation reference culture USDA1002 is better than Sinorhizobium nitrogen-fixing bacteria strain H6.However, under salt treatment, in inoculation
The effect that magnificent nodule azotobacter strain H6 increases root potassium content is significantly better than reference culture USDA1002, and is considerably better than not
Inoculation handles (table 2)；
Influence by Fig. 7 inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 to clover overground part and root phosphorus content：Comparison does not connect
The plant of bacterium, in the case where salt treatment is not added, inoculation reference culture USDA1002 and Sinorhizobium nitrogen-fixing bacteria strain H6 can increase lucerne
The overground part and root phosphorus content of Mu, and effect is identical both in lucerne 3 in salt-enduring cultivars, and salt density value kind Ah
In Er Gangjin, effect of the overground part phosphorus content in inoculation reference culture USDA1002 is better than Sinorhizobium nitrogen-fixing bacteria strain H6.So
And under salt treatment, the effect that inoculation Sinorhizobium nitrogen-fixing bacteria strain H6 increases root phosphorus content is significantly better than reference culture
USDA1002, and be considerably better than and be not inoculated with processing (table 2).
A kind of Sinorhizobium nitrogen-fixing bacteria strain H6 of the present invention, shows through experimental study：In the case where adding salt treatment, use
Sinorhizobium nitrogen-fixing bacteria strain H6 has obvious effect of increasing production, biomass, nitrogen content, potassium content and the phosphorus content of clover
Etc. indexs be above the plant not being inoculated with, and be higher than inoculation reference culture USDA1002, show to be inoculated with Sinorhizobium nitrogen-fixing bacteria
Strain H6 can help plant to alleviate salt damage.It can be seen that Sinorhizobium nitrogen-fixing bacteria strain H6 can be used for Xinjiang salinization soil area
The popularizing application of large area in ALFALFA PRODUCTION.
Sequence table
<110>Xinjiang Agricultural Univ
<120>Sinorhizobium nitrogen-fixing bacteria strain H6 and its application
<130> 2018
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1849
<212> DNA
<213>Sinorhizobium nitrogen-fixing bacteria strain H6 (Sinorhizobium sp.)
<400> 1
cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg cagaacctta ccagcccttg 60
acatcccgat cgcggatacg agagatcgta tccttcagtt cggctggatc ggagacaggt 120
gctgcatggc tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 180
aaccctcgcc cttagttgcc agcattcagt tgggcactct aaggggactg ccggtgataa 240
gccgagagga aggtggggat gacgtcaagt cctcatggcc cttacgggct gggctacaca 300
cgtgctacaa tggtggtgac agtgggcagc gagaccgcga ggtcgagcta atctccaaaa 360
gccatctcag ttcggattgc actctgcaac tcgagtgcat gaagttggaa tcgctagtaa 420
tcgcagatca gcatgctgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca 480
ccatgggagt tggttctacc cgaaggtagt gcgctaaccg caaggaggca gctaaccacg 540
gtagggtcag cgactggggt gaagtcgtaa caaggtagcc gtaggggaac ctgcggctgg 600
atcacctcct ttctaaggaa gctgtggaat tggaagacgg catcttcgga tgcatgacct 660
ttcccgtgct ttttagaaca tagatggcac cagtcaggtg accatcgaac gcaatacgtc 720
gagtatggcg atttggttgt ccttcgagcg ctaagcgaga agggcggata gccaaatccc 780
gctcagatat ggcgagaccc gccgtccacg tttctctttc tcacaaggat acgaaccacg 840
ccgcatcatg ttgcgtgctt aacgaatggg cccgtagctc aggtggttag agcgcacgcc 900
tgataagcgt gaggtcggca gttcgagtct gcccgggccc accattcgct tatgcgaatg 960
tgtcctgacg ctgtcgcgat ctaaagatcg cttcgctccg gacgggccgc cgaacgatcg 1020
gcgacgggct ttgcccttgc ggcgctatgc gccgtcgggt gcacgacgtg ttcacccgaa 1080
tatggggctg tagctcagct gggagagcac ctgctttgca agcagggggt cagcggttcg 1140
atcccgctca gctccaccat tcgattggtg ttgaactggc ggatattatc cttggagaaa 1200
aaataggttt gcatcgtctc catcgtgaga cggatgcctg ttctgcttac attgtgaaga 1260
gaagatatgt ctggaagctt ccaggtgttg atggtgatcg aaaggttgcc tgagacgtcc 1320
gagcccagtc ccgaagaagc catggatggt ctagccgacc gaaaatggaa gagggtctgg 1380
aggtaggagg gaagtcttgt cctttgaggc atgatcgttg ttggggtgtt cttcggaacg 1440
cgcctctgat ggtcgtgtcg gattggtgtt gcctgaccgc gcatcaccgg acagatctcg 1500
agaagctggt cttaagatat ggcctcgaag tcgctcggcg tgacttttgt gaggaccata 1560
tcgaacacgt cgatggcatt gttagaaagc gcgattgtaa aaggtaatcg cgcatagctg 1620
gtctggcgac tgcaaggtct ccggattttg gctgctgcgg catacgaaag ttgcccggat 1680
ggccagattt ggcacccctt cgagcgcaag cgaggaggat aggtttgcca aatccagcaa 1740
agacgatgag cattggcaat gagaacgatc aagtgaaaga agggcatttg gtggatgcct 1800
tggcatgcac aggcgatgaa ggacgtgata cgctgcgaaa agccgtggg 1849
Claims (2)
1. a kind of Sinorhizobium nitrogen-fixing bacteria strain H6, it is characterised in that Sinorhizobium H6Sinorhizobium sp.H6Belong to
In Sinorhizobium Pseudomonas, Wuhan University China typical culture collection center CCTCC, preservation were preserved on May 07th, 2018
Number is CCTCC NO:M 2018255.
2. Sinorhizobium nitrogen-fixing bacteria strain H6 as described in claim 1 is preparing legume symbiosis fixed nitrogen and is alleviating in salt damage
Application.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810684498.6A CN108841748B (en) | 2018-06-28 | 2018-06-28 | Sinorhizobium azotobacter strain H6 and application thereof |
Applications Claiming Priority (1)
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| CN110904005A (en) * | 2019-12-09 | 2020-03-24 | 江苏省农业科学院 | Fomesafen degrading bacterium and application thereof |
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| CN112358977A (en) * | 2020-08-04 | 2021-02-12 | 西安医学院 | Alfalfa rhizobium with growth promoting effect and separation and application method thereof |
| CN112358977B (en) * | 2020-08-04 | 2023-05-26 | 西安医学院 | Rhizobium meliloti with growth promoting effect and separation application method thereof |
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